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some helminthozoonotic infections in zambia.parasitic infections constitute an important group of zoonoses. stool examination of inpatients, medical examinees and out-patients in a sub-tropical environment established that inermicapsifer madagascariensis and schistosoma mattheei are occasionally anthropozoonotic in zambia. strongyloides fülleborni, a species normally parasitic in primates is not uncommon in man; whether it is anthropozoonotic or a parasite well adapted to man in this part of africa needs further investigation. the finding ...197897951
further observations on immunization of sheep against schistosoma mansoni and s. bovis using irradiation-attenuated schistosomula of homologous and heterologous species.this paper describes further characteristics of the immunization of sheep against schistosomes using live, irradiation schistosomula. sheep immunized with a non-virulent strain of schistosoma mattheei were protected against a more virulent strain of the same species for over a year. as there was no evidence that the irradiated parasites were able to persist this long, it was concluded that the vaccine had induced a sterile resistance. heterologous vaccination, using irradiated s. mattheei schist ...1979471535
the epidemiology of schistosomiasis in the vicinity of lake sibaya, with a note on other areas of tongaland (natal, south africa).the epidemiology of human and bovine schistosomiasis in the lake sibaya area of tongaland, south africa, an undeveloped rural environment, is discussed. the mean prevalence of schistosoma haematobium infection is 72%, but s. mansoni is absent; possible reasons for this are given and the different types of water habitat are shown to play different roles in transmission. fear of crocodiles and hippopotami is important because villagers are compelled to use for domestic purposes, the smaller, shall ...1979539856
studies on the pathogenesis of a strain of schistosoma mattheei maintained in hamsters. 1977599264
modification of the pathogenicity of schistosoma mattheei for sheep by passage of the parasite in hamsters.border leicester x suffolk sheep infected with a strain of s. mattheei maintained in hamsters do not develop the same pathological changes as romney marsh sheep infected with the same strain of parasite before hamster passage. to determine the cause of this reduced pathogenicity, five romney marsh sheep were each infected with 10 000 cercariae of the hamster-passaged parasite and five with 10 000 cercariae of a s. mattheei strain from onderstepoort, south africa, passaged exclusively through she ...1977599276
immunization of sheep against a virulent strain of schistosoma mattheei using a strain of s. mattheei attenuated by hamster passage.we have previously described the characteristics of a relatively non-pathogenic laboratory strain of s. mattheei, attenuation of which was apparently caused by passage in hamsters. we now show that chronic infection with this avirulent strain largely protects sheep from the manifestations of acute schistosomiasis when challenged with a virulent strain of s. mattheei. four sheep were each infected with 10 000 cercariae of the avirulent strain and, together with four wormfree sheep, challenged 63 ...1977599277
schistosoma mattheei in the ox: clinical pathological observations.twenty-eight friesian calves were infected between seven and 11 months of age with 5000 to 45,000 cercariae of schistosoma mattheei. they developed anaemia, lymphopaenia and hypoalbuminaemia during the period of acute clinical illness after the infection became patent, and lymphocyte counts remained depressed after clinical recovery. neutrophil counts rose and later fell before returning to normal. eosinophilia and hypergammaglobulinaemia were marked during the period of recovery. the changes in ...1977605295
schistosoma mattheei in the ox: the serological response.thirty friesian steers were infected with schistosoma mattheei and the antibody response was followed for up to 76 weeks by the complement fixation (cf), indirect haemagglutination (ih) and indirect immunofluorescent (if) tests. cf and if antibodies rose to a peak at about 25 weeks and then fell, while ih antibodies rose more slowly and remained high. peak ih and if titres were proportional to the level of infection. peak cf titres were reduced in animals on a low plane of nutrition. there was a ...1977605296
the pathology of schistosoma mattheei infection in the ox 1. lesions attributable to the eggs. 1978621296
the pathology of schistosoma mattheei infection in the ox. 2. lesions attributable to the adult parasites. 1978621301
scanning electron microscopy of the integument of schistosoma mattheei veglia & le roux, 1929. 1977847868
schistosoma mettheei infestation in the ox: the intestinal syndrome.twenty-eight friesland calves were infested at 7 to 11 months of age with 5 000-45 ooo cercariae of schistosoma mattheei. at 7 to 8 weeks post-infestation the majority developed an acute intestinal syndrome characterised by diarrhoea or dysentery, anorexia and loss of condition, from which they recovered spontansously. the severity and duration of illness was proportional to the level of infestation. two heavily infested animals on a low plan of nutrition developed a subacute syndrome in which t ...1977874950
schistosoma mattheei in the ox: the chronic hepatic syndrome.a friesland steer infested on four occasions at intervals of 4--6 weeks with 20 000 cercariae of schistosoma mattheei developed progressive hepatic failure and died after 74 weeks. the condition was characterised by enlargement and induration of the liver with portal fibrosis, inflammation of the portal veins and "piecemeal necrosis", and was associated with a severe circulating eosinophilia and hypergammaglobulinaemia. similar cases were encountered in two natural outbreaks. the syndrome is con ...1977915892
the globule leucocyte in bovine schistosomiasis.globule leucocytes were detected in forestomachs, abomasum, duodenum, ileum, large intestine, lung, liver, bladder and kidney in cattle infected with schistosoma mattheei. in animals examined seven and eight weeks after infection they were found only in the lungs. they were present in the alimentary tract from 18 weeks onwards. they were most numerous and most widely distributed in animals subjected to repeated, heavy infection. there was evidence that they were associated with the immune respon ...1977928987
studies on schistosomiasis. 8. the influence of age on the susceptibility of sheep to infestation with schistosoma mattheei.twenty-eight dorper wethers, allocated according to age into 4 groups of 7 animals each, and 1 group of 7 merino wethers, were compared for susceptibility to schistosoma mattheei infestation. the group mean ages of the dorper sheep varied from 5-61 months and their live mass from 25-66 kg, while the merinos were 8 months old and had a mean mass of 19 kg. despite the marked differences in the age and live mass of the dorper sheep and the inclusion of 2 breeds in the experiment, no statistically s ...19761018889
excretion of schistosoma mattheei eggs from man, baboons and cattle living in their normal environment.egg excretion of s. mattheei was studied for about a year from man, baboons and cattle living in their normal environment. although the excretory pattern was not unequivocal from man, there was a tendency of egg counts to decline with time: the baboons showed a seasonal pattern and the cattle a stable pattern throughout the year.19751151051
studies on schistosomiasis. 7. a comparison of various methods for the infestation of sheep with schistosoma mattheei.the percutaneous (leg and thorax and abdomen) and subcutaneous routes of infestation with schistosoma mattheei were compared in 29 sheep. larger percentages of cercariae developed after percutaneous than subcutaneous infestation and the difference was highly significant (p less than 0,0001). furthermore, if the leg was used for percutaneous infestation worm development was significantly higher (p less than 0,02) when the skin was washed thoroughly in water before exposing it to cercariae, than w ...19751208043
an apparatus for the restraint of sheep during infestation with schistosomes.with this adjustable apparatus sheep aged from five months to five years and varying in mass from 16-95 kg could be restrained effectively. cercarial suspension was split by only 2/44 sheep during infestation with schistosoma mattheei. using a battery of seven cages, it was possible to infest 23 sheep, each for 30 minutes, in one day. no cercariae were more than 4-1/2 hours old at the end of exposure.19751208044
observations on schistosoma mattheei veglia and le roux, 1929 infections in man in zambia. 19751231667
immunisation of sheep against schistosoma mattheei using either irradiated cercariae or irradiated schistosomula.irradiated cercariae, irradiated schistosomula, or heterologous infections were used to vaccinate sheep against schistosoma mattheei infection. in the first experiment four doses of 10(4) s. mattheei cercariae irradiated at 6kr were administered to sheep by percutaneous infection at 4 week intervals. this induced a 74% reduction in a challenge infection compared to control sheep while only 13% protection was achieved in a third group of sheep immunised with normal cercariae of the heterologous p ...19761262686
parasites of south african wildlife. xii. helminths of nyala, tragelaphus angasii, in natal.the helminths of 77 nyala, tragelaphus angasii, from 4 game reserves in natal, were collected, counted and identified. ten nematode species and 4 nematode genera, a trematode species and paramphistomes, and 2 cestode genera were recovered from the antelope. of these, a cooperia rotundispiculum race, gaigeria pachyscelis, a gongylonema sp., haemonchus vegliai, impalaia tuberculata, an oesophagostomum sp., a setaria sp., trichostrongylus deflexus, trichostrongylus falculatus, the larval stage of a ...19911780128
the regulation of mortality and fecundity in schistosoma mattheei following a single experimental infection in sheep.the regulation of mortality and fecundity of schistosoma mattheei in sheep was examined using a series of mathematical models applied to data culled from the literature. parasite mortality (mu) was found to be an increasing linear function of the magnitude of the initial infection over the ranges of doses examined (200-91,000 cercariae) where mu = 9.78 x 10(-3) + 3.476 x 10(-7) infection dose. parasite fecundity (lambda) was found to be inversely related to the duration of the infection. the bes ...19911787027
occurrence of schistosoma mattheei-like eggs in a spinal cord lesion. 19911887479
adherence of blood platelets and leucocytes to hybrid schistosomes.the bovine parasite, schistosoma mattheei was crossed with the human schistosome, s. haematobium. the f1 hybrids resulting from this cross were viable in both snails and rodents. however, f1 x f1 (f2) crosses were less viable in snails and a proportion of them seemed to be changed structurally when viewed by scanning electron microscopy. certain of the schistosomes were covered with a dense mass of interconnected blood platelets resembling a temporary haemostatic plug but not a blood clot. inter ...19902074140
frequency and possible consequences of hybridization between schistosoma haematobium and s. mattheei in the eastern transvaal lowveld.a survey was conducted at a locality in the eastern transvaal lowveld where the prevalence of human infection with the bovine parasite, schistosoma mattheei, is relatively high. it was found that, when compared to the number of s. haematobium eggs released into the environment, the number of s. mattheei eggs, with enclosed hybrid miracidia, is small. the consequences of backcrossing between the hybrids and s. haematobium was considered; a mathematical model indicated that a high percentage of th ...19902126549
do all human urinary infections with schistosoma mattheei represent hybridization between s. haematobium and s. mattheei?enzyme electrophoresis indicated that all schistosoma mattheei eggs passed in the urine of humans derive from s. mattheei females in copula with s. haematobium males. it appears that s. mattheei males do not reach sexual maturity in man; however, s. haematobiumxs. mattheei males possibly do.19902149382
host cellular components adhering to the tegument of schistosomes from cattle, buffalo, hippopotamus and lechwe.the teguments of adult schistosoma mattheei from cattle and buffalo, s. hippopotami from hippopotamus and s. margrebowiei from lechwe were examined by means of scanning electron microscopy for cells possibly engaged in immunological action. leukocytes were observed on the teguments of the schistosomes from all 4 host species. although certain of these cells seemed to be fused to the surface membrane of the worms, they did not display pseudopodia. the tegument of certain schistosomes from buffalo ...19902216347
scanning electron microscopical observations on the shedding of the tegument of adult schistosoma mattheei.in search of indications of membrane turnover the teguments of male schistosoma mattheei from cattle and laboratory rodents were studied by means of scanning electron microscopy. a number of slightly elevated circular patches of tegument which appeared to peel off on the edges were seen on the outer membrane of a limited number of specimens from both rodents and cattle. it is suggested that this phenomenon may represent limited rapid turnover of the outer layer in response to host immunological ...19902276869
an experimental schistosoma mattheei infection in man.certain aspects of the immune response of a male experimentally infected with 3-day old cercariae of a pure field strain of schistosoma matheei were investigated. among others, aspects such as the reaction of eosinophils, neutrophils and blood platelets after infection, were included in the study. the involvement of igg and the cross reaction between these antibodies and s. haematobium and s. mansoni were also investigated. the phenomenon that the cercariae were, 3 days after shedding, still cap ...19902293129
enzyme polymorphism in schistosoma mattheei from cattle in the eastern transvaal lowveld.enzyme electrophoresis was conducted on 10 schistosoma mattheei adult worm samples, comprising 270 individuals, collected from cattle in the eastern transvaal lowveld. glucose-6-phosphate dehydrogenase (g6pdh) was studied in all the samples and phosphoglucomutase (pgm) and malate dehydrogenase (mdh) in five populations each. only one population was polymorphic for g6pdh. in this population, in addition to the allele found in all the other samples, a second allele occurred with a similar rf value ...19892529308
parasites of south african wildlife. iv. helminths of kudu, tragelaphus strepsiceros, in the kruger national park.during ongoing surveys of parasites of wild animals in the kruger national park, a total of 96 kudu. tragelaphus strepsiceros, were culled in the southern part of the park at monthly intervals from april 1981 to march 1983. a single kudu was shot in the same area in november 1983. two more kudu were obtained from pafuri in the northern part of the park during october 1981 and another from near satara in the central part of the park during october 1982. the helminths of all the kudu were collecte ...19892748130
notes on the occurrence of tubercular spines in schistosoma margrebowiei and schistosoma mattheei.scanning electron microscopical (sem) studies on the tegument of the bovid schistosomes, schistosoma margrebowiei and schistosoma mattheei have yielded conflicting results; certain authors observed the tubercles on the tegument of these species to be spined, while others reported that they are spineless. the present study indicates that the protrusion of tubercular spines is subject to phenotypic plasticity regulated by external factors such as the identity of the host species and whether or not ...19883194122
further observations on the electrophoretic characterization of south african schistosoma mattheei and s. haematobium.eleven enzymes, which were used to compare south african s. mattheei and s. haematobium in a former study, were employed to study intraspecific variation within s. mattheei, using starch gel electrophoresis and iso-electric focusing where resolution in starch gel was poor. acid phosphatase varied intraspecifically within s. mattheei in that the most southern population differed from the northern populations. malate dehydrogenase also varied intraspecifically. three populations which occur sympat ...19883353101
schistosomal lesions in the bovine uterus.inspection of bovine female genitalia at a major abattoir in north-eastern zimbabwe showed schistosome-induced granulomas in uterine walls. thirty-six non-pregnant and 7 pregnant uteri of 3441 examined, had lesions either in the mid-dorsal aspect of the uterine body or in the horns. the reproductive history of the cows was not known but the 7 pregnancies appeared normal. lesions were most severe in the myometrium and consisted of rings of multinucleated giant cells and macrophages occurring arou ...19883417918
prevalence and distribution of schistosoma mattheei in zimbabwe. 19873426311
the occurrence of schistosoma mattheei in the south-western transvaal.to determine whether schistosoma mattheei is present in the south-western transvaal, sixty habitats were searched for the intermediate host snail, bulinus africanus. ten populations of this snail were located, 2 of which were infected with s. mattheei. eggs of one of these isolates, originating from a spring in the mooi river, were examined with an optical microscope. scanning electron micrographs of the teguments of adult male worms and the terebratorial membranes of miracidia are described. th ...19873444616
enzyme electrophoresis of south african schistosoma mattheei and s. haematobium.as a pilot project of a study undertaken to determine the influence of s. mattheei x s. haematobium hybridization on various south african s. mattheei populations by means of biochemical-taxonomic methods, a comparative electrophoretic study of laboratory-maintained s. mattheei and s. haematobium was performed, using 11 enzymes representing 16 gene loci. eleven loci were found to be monomorphic, while 5 differed interspecifically. computation of the results revealed that south african s. matthee ...19873587936
phenotypic differences in schistosoma mattheei ova from populations sympatric and allopatric to s. haematobium.schistosoma mattheei ova were collected from cattle in different localities in south africa and after hatching, miracidia were used to infest bulinus (physopsis) globosus. cercariae harvested from these snails were used to infest the definitive host praomys (mastomys) coucha and eggs from the resulting female s. mattheei were collected. these ova were compared with a schistosoma haematobium x s. mattheei hybrid similarly collected from an infested p. (m.) coucha. the results indicate that s. mat ...19863725328
scanning electron microscopy of the teguments of males from five populations of schistosoma mattheei.the teguments of males from 5 populations of s. mattheei, of which 3 were sympatric and 2 allopatric with s. haematobium, were studied by means of scanning electron microscopy (sem). a certain percentage of the males of each sympatric population bore tubercle spines while the allopatric populations were spineless. it is postulated that the presence of tubercle spines is a characteristic inherited from s. haematobium.19863725329
further observations on an intratubercular sensory receptor of schistosoma mattheei.a structure, presumably a sensory receptor in the nippled tubercles of schistosoma mattheei, previously observed by scanning electron microscopy, was studied further by light and transmission electron microscopy. the results obtained by differential staining indicate that this structure does, in fact, consist of nervous tissue, and this provides additional evidence to support the sensory receptor hypothesis.19863796951
an immunochemical investigation of the allergens from ascaris suum perienteric fluid. cross-reactivity, molecular weight distribution and correlation with phosphorylcholine-containing components.an extract of ascaris suum perienteric fluid was fractionated on sephadex g-200 and the resulting high molecular weight (approx. 600,000 daltons) and low molecular weight (approx. 16,000 daltons) fractions examined for allergenicity using human sera. radioallergosorbent (rast) and rast-inhibition assays showed that the high molecular weight fraction was 15- to 40-fold more potent than the low molecular weight fraction. crossed radio-immunoelectrophoretic analysis showed that the high molecular w ...19873804458
the morphology of a sensory receptor in the nippled tubercles of schistosoma mattheei.during scanning electron microscopy (sem) of the tegument of schistosoma mattheei, a structure was observed within the nippled tubercles. it is postulated that it is a sensory receptor with a tactile function.19854047617
serum protein fractions as determined by cellulose actate electrophoresis in schistosoma mattheei infested sheep. 19744137925
"parthenogenesis" in schistosoma mattheei. 19694191601
observations on the ecology of schistosoma mattheei veglia & le roux, 1929, in portion of the kruger national park and surrounding area using a new quantitative technique for egg output. 19734212207
schistosoma mattheei in sheep: the host-parasite relationship. 19744473093
first recorded finding of ova of the cattle parasite schistosoma mattheei in human cervical smears. 19744473101
patho-physiology of ovine schistosomiasis. v. onset and development of anaemia in sheep experimentally infected with schistosoma mattheei--studies with 51cr-labelled erythrocytes. 19744546877
patho-physiology of ovine schistosomiasis. vi. onset and development of anaemia in sheep experimentally infected with schistosoma mattheei--ferrokinetic studies. 19744546878
studies on immunity to reinfection with schistosoma mattheei in sheep and cattle.few experiments have been reported on immunity to reinfection in domestic stock. the degree of resistance to reinfection with s. mattheei was studied in cattle and sheep. on the basis of the results, the only evidence of acquired immunity in calves and sheep is a reduction in the length of adult worms.19744549200
studies on schistosomiasis. 3. detection of antibodies against schistosoma mattheei by the indirect immuno-fluorescent method. 19724577546
patho-physiology of ovine schistosomiasis. ii. some observations on the sequential changes in blood volume and water and electrolyte metabolism following a single experimental infection of schistosoma mattheei. 19734727897
patho-physiology of ovine schistosomiasis. i. a clinico-pathological study of experimental schistosoma mattheei infections. 19734737980
patho physiology of ovine schistosomiasis. 3. study of plasma protein metabolism in experimental schistosoma mattheei infections. 19734772088
pathophysiology of ovine schistosomiasis. iv. effects of experimental schistosoma mattheei infection on the nitrogen status and digestive efficiency of the host. 19734772089
schistosoma mattheei in cattle: the host-parasite relationship. 19734805162
schistosoma mattheei in cattle: variations in parasite egg production. 19734805163
observations on the infection of bulinid snails with schistosoma mattheei. ii. the mechanism of resistance to infection. 19715550313
observations on the infection of bulinid snails with schistosoma mattheei. i. the susceptibility of bulinus africanus and bulinus truncatus. 19685714964
maturation and egg production by schistosoma mattheei females in unisexual and cross specific infections. 19695789094
false-positive reactions in the serological diagnosis of schistosomiasis.stool and urine samples from children living in an area in the western transvaal in which human schistosomiasis was not endemic were examined for parasites and the indirect fluorescent antibody test was performed on their sera. since none of these children passed any schistosome ova in their excreta but approximately half of them had a positive serological reaction they must have been infected with either schistosoma mattheei, which is common in snails and cattle in the area, or avian schistosom ...19836348970
schistosoma mattheei veglia & le roux, 1929, egg output from cattle in a highly endemic area in the eastern transvaal.the results of 6-month estimations of s. mattheei faecal egg counts on 513 cattle in a highly endemic area of the eastern transvaal over a 2-year period are given. after an initial high egg output of short duration the egg counts stabilized at a low level. the frequency of high egg counts in young cattle which died naturally was more than twice that of all other cattle, suggesting that s. mattheei egg counts in highly endemic areas is debatable, and it is suggested that egg counts in man might f ...19827185039
studies on schistosomiasis. 10. development of schistosoma mattheei in sheep infested with equal numbers of male and female cercariae. 19817312309
the pathogenesis of schistosoma mattheei in the sheep.nine dorper lambs infected with 3000 cercariae of schistosoma mattheei showed inappetence, reduced growth rate, anaemia, hypoalbuminaemia, hyperglobulinaemia and an intermittent eosinophilia. a marked granulomatous reaction in the intestinal mucosa was associated with the deposition and accumulation of eggs. the disease was progressive for the first 25 weeks and three sheep died or were slaughtered in extremis between 12 and 24 weeks after infection. in those animals that survived, the disease b ...19807455337
schistosoma mattheei infections in cattle: changes associated with season and age.the schistosoma mattheei egg output was monitored in 31 cattle over a 18-month period on a dairy farm near lusaka (zambia). the animals were kept on pasture with free access to two streams which were suitable for the intermediate host, bulinus globosus. individual faecal egg excretion reached an average peak of 130 eggs per gram, around 9 months after birth and decreased markedly before the age of 18 months. average counts declined significantly with age, down to less than five eggs per gram in ...19957660567
cattle schistosomiasis in zambia.a total of 358 cattle was examined for schistosome infection in zambian slaughterhouses. a total of 542 worms collected from 104 infected individuals was examined for glucose-6-phosphate dehydrogenase and phosphoglucomutase using isoelectric focusing. the overall prevalence of infection was 51%. ninety three percent of the infected animals had less than 100 worm pairs in the mesenteric veins. schistosoma mattheei was the predominant species (75%); s. leiperi (12%) and s. margrebowiei (2%) were a ...19947706674
the effect of experimental schistosoma mattheei infection on hepatic drug metabolism in balb/c mice. 19947707495
repetitive sequences in the ribosomal dna internal transcribed spacer of schistosoma haematobium, schistosoma intercalatum and schistosoma mattheei. 19948183315
circulating antigen levels in serum of cattle naturally infected with schistosoma mattheei.levels of 2 schistosoma circulating antigens, the circulating anodic antigen (caa) and the circulating cathodic antigen (cca), were determined in serum samples collected, on a monthly basis over a period of 1.5 years, from 32 farm animals of different ages and from 12 tracer calves exposed to schistosoma mattheei infection on a zambian farm. faecal egg counts were monitored in all animals and worm burdens in tracers determined after perfusion. antigen determination tests in serum, with sensitivi ...19968893532
variations in schistosoma mattheei egg morphology and viability according to age of infection in cattle.comparison of the numbers of schistosoma mattheei eggs and miracidia per gram faeces in groups of naturally infected calves, heifers and adult cows showed that the reduction in faecal egg excretion recorded as infection progresses is associated with a decline in the ability of eggs to hatch. while 50% of the eggs from calves produced a miracidium, only 15% of those excreted from adult cows did the same. the decline in egg viability is at least partly associated with morphological changes of the ...19968960223
schistosoma mattheei infection in cattle: the course of the intestinal syndrome, and an estimate of the lethal dose of cercariae.three groups of young oxen were infected percutaneously with cercariae of schistosoma mattheei. three of five oxen infected with 248 cercariae kg-1 mass died or were killed in extremis 58-70 d after infection, a fourth survived extremely severe clinical schistosomosis and the fifth was only slightly affected. none of seven calves infected with 187 cercariae kg-1 died, while one of seven exposed to 119 cercariae kg-1 was in extremis (possibly not from schistosomosis) when killed after 378 d. the ...19979204506
relationship of impairment of schistosome 28-kilodalton glutathione s-transferase (gst) activity to expression of immunity to schistosoma mattheei in calves vaccinated with recombinant schistosoma bovis 28-kilodalton gst.sera from calves vaccinated with the recombinant schistosoma bovis-derived 28-kda glutathione s-transferase (28gst) and subsequently naturally or experimentally exposed to schistosoma mattheei were studied for their content of specific immunoglobulin g (igg) and iga antibodies to recombinant s. bovis 28gst as well as for their capacity to inhibit the enzymatic activity of the antigen. the results were analyzed in regard to the presence (natural infection) or absence (experimental infection) of a ...19989488407
use of a pre-selected epitope of cathepsin-l1 in a highly specific peptide-based immunoassay for the diagnosis of fasciola hepatica infections in cattle.a peptide-based indirect elisa to detect cattle antibodies against fasciola hepatica was developed and evaluated for its sensitivity and specificity. an immunogenic antigen released in vitro by f. hepatica was purified. after purification the sequence of the first 20 n-terminal aa of this protein showed considerable homology with cathepsin l-like proteinase. based on its homology with cathepsin-l1, we further focused on this protein for diagnostic purpose. predicted b-cell epitopes of cathepsin- ...199910404262
natural infection with schistosomes of the schistosoma haematobium group in a dog in zambia.post-mortem examination of an adult male jack russell dog from zambia revealed that it was heavily infected with schistosomes. the dog had been admitted, with a history of retching, 4 days before its death. at necropsy, the liver was found to be enlarged, with multiple pin-point yellowish-white foci scattered diffusely throughout the organ. multiple pin-point recent and old haemorrhages were seen on the mucosal surface of the gastrointestinal tract, particularly in the stomach and proximal duode ...200010684694
observations on worm population dynamics in calves naturally infected with schistosoma mattheei.the evolution of faecal egg output, worm burdens and tissue egg counts in young calves was monitored during the first year of natural exposure to schistosoma mattheei infection on a zambian farm. according to the duration of their stay on the farm, these calves were classified into 2 groups of 14 temporary tracers (tt calves) which were introduced on a 2-monthly basis for residential periods of 2 months, and 12 permanent tracers (pt calves) introduced either at the beginning of the experiment (g ...199511023413
the relationship between faecal egg counts, worm burden and tissue egg counts in early schistosoma mattheei infections in cattle.the relationships between schistosome faecal egg counts (epg), total tissue egg counts (tec) and adult female worm burdens (fw) recorded at post-mortem examination of 30 friesian calves from three different field trials were analysed. the calves in study 1 (n=14) had been exposed to natural schistosoma mattheei infections for 2 months, those of study 2 (n=9) for between 4 and 12 months, and those in study 3 (n=7) for 8 months. no clinical schistosomiasis was observed in any of the groups, and at ...200211755433
polymerase chain reaction assay based on a highly repeated sequence of schistosoma haematobium: a potential tool for monitoring schistosome-infested water.we have cloned from schistosoma haematobium genome a repeated sequence, the drai repeated sequence, which consists of tandemly arranged 121-bp-long units and which is highly abundant (approximately 15% of the s. haematobium genome). by these features, the drai repeat is similar to the sm1-7 sequence of schistosoma mansoni previously described by us. however, their nucleotide sequences are profoundly different. polymerase chain reaction (pcr) primers were designed on the basis of the drai sequenc ...200111791997
susceptibility of freshwater snails to the amphistome calicophoron microbothrium and the influence of the species on susceptibility of bulinus tropicus to schistosoma haematobium and schistosoma mattheei infections.the susceptibility of bulinus tropicus, b. globosus, biomphalana pfeifferi, lymnaea natalensis, and melanoides tuberculata to calicophoron microbothrium was examined. bulinus tropicus had the highest prevalence (65.0%), followed by b. pfeifferi (37.5%), b. globosus (6.8%), and m. tuberculata (5.9%). lymnaea natalensis was refractory to infection. bulinus tropicus snails infected with c. microbothrium alone or coinfected with either schistosoma haematobium or s. mattheei 0, 7, 14, and 21 days aft ...200212435124
the influence of colostrum on early schistosoma mattheei infections in calves.the study investigated whether the susceptibility of calves to an early schistosoma mattheei infection may be modified by intake of colostrum from infected cows. twelve calves born to non-infected mothers were randomly divided into 2 groups of 6. the animals from group 1 were fed colostrum originating from a pool collected from non-infected cows, the calves from group 2 received colostrum from a pool collected from cows infected with s. mattheei. one month after birth all calves were infected by ...200212553572
transplacental transfer of schistosomal circulating anodic antigens in cows.the present work investigated the transplacental passage of circulating anodic schistosome antigens (caa) and the production of foetal antibodies in response to antigenic stimulation in schistosoma mattheei infected cows. three groups were available: six calves born to non-infected cows received colostrum from a pool from non-infected cows (group 1), six calves born to non-infected cows (group 2) and six calves born to infected cows (group 3) received colostrum from a pool from infected cows. sc ...200212694602
schistosoma mattheei--an ovum containing twin miracidia.a large schistosoma mettheei ovum containing two miracidia was recovered from a squash preparation of the liver of an experimentally infected hamster. when observed, the miracidia were motile and facing in opposite directions.200312825683
description and classification of abnormal cercariae of schistosoma mattheei and a method of concentration and collection.over a period of about 12 years, 30 abnormal schistosoma mattheei cercariae were found among a total of approximately 2.8 million examined. initially seven were recovered from about 1.02 million (0.0007%), which were examined individually while being counted with the aid of a stereoscopic microscope. subsequently, on the strength of relatively high percentages of abnormal individuals recovered when counting cercariae that failed to penetrate into oxen, it appeared that the morphologically abnorm ...200312967173
variations in the immune response to natural schistosoma mattheei infections in calves born to infected mothers.during previous work schistosoma antibodies and circulating antigens were detected at birth in the serum from some calves born to schistosoma mattheei infected mothers. the objectives of the present survey were: (1) to investigate the proportion of calves, born to cows infected with s. mattheei, which have specific antibodies and circulating schistosome antigens present in their serum at birth and (2) to investigate whether the presence or absence of these specific antibodies and/or circulating ...200414746977
placental transfer of immunoglobulins in cattle infected with schistosoma mattheei.although the epitheliochorial placenta of ruminants does not allow passage of immunoglobulins from dam to foetus specific antibodies have been detected at birth in calves born to schistosoma mattheei-infected cows. the present study determined the prevalence of calves born with specific antibodies for s. mattheei and the origin of these antibodies. for the determination of the prevalence, 100 calves born to infected mothers in an endemic area (zambia) were examined, 24 were seropositive. to stud ...200515734547
the influence of colostrum on infection of calves around 7 months of age with schistosoma mattheei.studies have indicated that the intake of colostrum could modulate the offspring reaction towards early schistosome infections. the effect of colostrum (containing immunoglobulins, parasite antigens, immune cells and other cell-related products) on late schistosoma infections is to our knowledge not documented. the objective of the present study is to determine whether the intake of colostrum from schistosoma mattheei infected cows will modify late s. mattheei infection patterns in their offspri ...200515817203
epidemiology and control of trematode infections in cattle in zimbabwe: a review.in this paper the main epidemiological aspects of the major domestic ruminant trematode infections in zimbabwe are reviewed and discussed with regard to the available options for control. seasonal occurrence of amphistomes, fasciola gigantica and schistosoma mattheei are considered both in the definitive and intermediate hosts. the regional distribution of the trematodes is reviewed in relation to the distribution patterns of their snail intermediate hosts. based on the epidemiological features ...200515900894
distribution, habitats and role as intermediate host of the freshwater snail, bulinus forskalii, in south africa.this paper focuses on the geographical distribution and habitats of bulinus forskalii, the snail intermediate host of the conical fluke of equids, gastrodiscus aegyptiacus as reflected by the 1209 samples in the database of the national freshwater snail collection of south africa. the 362 different loci on record represent an extensive distribution in kwazulu-natal province, the limpopo province, the coastal areas of the eastern cape province and the south-eastern part of the north west province ...200516137134
immunity to schistosomiasis in animals: an update.the present paper reviews the available literature on the development of immunity to animal schistosoma infections. the majority of the studies on animal schistosomiasis were performed in cattle and pigs and only schistosoma mattheei, s. bovis and s. japonicum received particular attention, mainly because of their recognized veterinary significance or zoonotic aspect. although it is an accepted fact that acquired resistance to schistosoma is of major importance in the regulation of infection int ...200516138850
epidemiological studies of schistosoma mattheei infections in cattle in the highveld and lowveld communal grazing areas of zimbabwe.during the period between january 1999 and december 2000, the distribution and seasonal patterns of schistosoma mattheei infections in cattle in the highveld and lowveld communal grazing areas of zimbabwe were determined through monthly coprological examination. faecal samples of cattle were collected from 12 and nine dipping sites in the highveld and lowveld communal grazing areas, respectively. patterns of distribution and seasonal fluctuations of the intermediate host-snail populations and th ...200617058440
perinatal priming of calves born to schistosoma mattheei-infected dams.the objective of this study was to elucidate whether calves born to infected dams had been primed against schistosoma mattheei antigens. infection-confirmed, pregnant cows were randomly selected for monitoring their offspring. pre-colostral serum was collected from the neonates for the detection of specific antibodies at birth, as they indicate a transplacental transfer of schistosome-specific antibodies and antigen. at the age of approximately 2 months, peripheral blood mononuclear cells (pbmc) ...200717084532
molecular identification of schistosoma mattheei from feces of kinda (papio cynocephalus kindae) and grayfoot baboons (papio ursinus griseipes) in zambia.terminal-spined schistosoma sp. eggs were detected in several groups of baboons living in kafue national park in central zambia. a total of 166 fecal samples was screened; egg prevalence overall ranged between 7% and 10%, while infection intensities were low. formalin-fixed eggs had an average length of 144.5 microm and a breadth of 48.3 microm, but the schistosome species could not be unambiguously identified by size or morphology. we used molecular methods to definitively identify the parasite ...201019697970
ca88, a nuclear repetitive dna sequence identified in schistosoma mansoni, aids in the genotyping of nine schistosoma species of medical and veterinary importance.ca88 is the first long nuclear repetitive dna sequence identified in the blood fluke, schistosoma mansoni. the assembled s. mansoni sequence, which contains the ca88 repeat, has 8,887 nucleotides and at least three repeat units of approximately 360 bp. in addition, ca88 also possesses an internal ca microsatellite, identified as smbr18. both pcr and blast analysis have been used to analyse and confirm the ca88 sequence in other s. mansoni sequences in the public database. pcr-acquired nuclear re ...201020721481
differentiating schistosoma haematobium from schistosoma magrebowiei and other closely related schistosomes by polymerase chain reaction amplification of a species specific mitochondrial gene.schistosoma haematobium infection afflicts about 150 million people in 53 countries in africa and the middle east. in many endemic areas, s. haematobium is sympatric with schistosoma bovis, schistosoma mattheei, schistosoma curassoni, schistosoma intercalatum and schistosoma magrebowiei, its closely related species. in addition, they also develop in the same intermediate snail hosts. since these schistosome species often infect snails inhabiting the same bodies of water, examining cercariae or i ...201424754026
adaptive radiation within the vaccine target tetraspanin-23 across nine schistosoma species from africa.high levels of polymorphism in dna sequences of tetraspanin-23 (tsp-23) were revealed within and between nine different species of schistosoma from africa including schistosoma mansoni, schistosoma rodhaini, schistosoma margrebowiei, schistosoma mattheei, schistosoma intercalatum, schistosoma haematobium, schistosoma guineensis, schistosoma curassoni and schistosoma bovis. the greatest levels of diversity coincided with evidence of positive selection (d(n)/d(s)>1) within regions that code for ex ...201323220042
differentiating schistosoma haematobium from related animal schistosomes by pcr amplifying inter-repeat sequences flanking newly selected repeated sequences.in schistosomiasis elimination programs, successful discrimination of schistosoma haematobium from the related animal schistosoma parasites will be essential for accurate detection of human parasite transmission. polymerase chain reaction assays employing primers from two newly selected repeated sequences, named sh73 and sh77, did not discriminate s. haematobium when amplifying sh73-77 intra- or inter-repeats. however, amplification between sh73 and the previously described drai repeat exhibited ...201223109375
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