| distribution and specific binding "in vivo" of iodinated growth hormones in the turtle chrysemys dorbigni. | | 1979 | 222646 |
| isolation, purification and primary structure of insulin from the turtle chrysemys dorbigni. | insulin a and b chains from pancreas of the turtle chrysemys dorbigni have been purified to homogeneity, and their primary structures have been determined. the sequence of the a chain is g-i-v-e-q-c-c-h-n-t-c-s-l-y-q-l-e-n-y-c-n, and that of the b chain is a-a-n-q-h-l-c-g-s-h-l-v-e-a-l-y-l-v-c-g-e-r-g-f-f-y-s-p-k-a. the amino acid sequence of chrysemys insulin is identical to that of another turtle (pseudemys scripta), the chicken, and turkey. when compared with alligator insulin, it has three c ... | 1991 | 1808015 |
| immunohistochemical localization of insulin-like materials in antral gastric mucosa and intestinal epithelial cells of the turtles chrysemys dorbigni and phrynops hilarii. | immunoreactive insulin was demonstrated immunohistochemically with antibodies to human and porcine insulin by the avidin-biotin-peroxidase complex method in open-type gastrointestinal cells from sections of the antral stomach and of the upper, middle and lower intestine of the turtles chrysemys dorbigni and phrynops hilarii. in both species the concentration of cells positive for insulin-like material was higher in the gastric antrum than in the gut. the localization of insulin-like material in ... | 1991 | 1823247 |
| in vitro effect of insulin on the uptake of glucose and alpha-aminoisobutyric acid in the thyroid gland of the turtle (chrysemys dorbigni). | the presence of specific insulin binding sites in the thyroid gland of the turtle chrysemys dorbigni has been previously reported. the purpose of the present work was to investigate the probable action of insulin on the uptake of [14c]deoxy-d-glucose ([14c]dg) and [14c]alpha-aminoisobutyric acid ([14c]aib) in turtle (c. dorbigni) thyroid glands in vitro. thyroid fragments (+/- 40 mg) were incubated at 25 degrees in krebs-ringer-bicarbonate buffer containing 0.2 microci of [14c]dg or [14c]aib wit ... | 1991 | 1874392 |
| specific uptake, dissociation, and degradation of 125i-labeled insulin in isolated turtle (chrysemys dorbigni) thyroid glands. | thyroid glands from turtles (chrysemys dorbigni) pretreated with potassium iodide were incubated with 125i-insulin in the presence or absence of unlabeled insulin, in order to study its specific uptake. at 24 degrees, the specific uptake reached a plateau at 180 min of incubation. the dose of bovine insulin that inhibited 50% of the 125i-insulin uptake was 2 micrograms/ml of incubation medium. most of the radioactive material (71%) extracted from the gland, after 30 min incubation with 125i-insu ... | 1985 | 3905504 |
| in vivo effect of temperature on the specific liver uptake and renal degradation of labeled human growth hormone in the turtle chrysemys dorbigni. | | 1981 | 7250680 |
| identification and initial characterization of serum growth hormone binding protein in the turtle chrysemys dorbigni. | proteins that bind growth hormone (ghbp) have been identified in the blood of many mammalian and avian species, but not in reptilian species. we carried out binding studies with the serum of turtles using chromatographic techniques as well as the dextran-charcoal separation method. as in other species, we found at least two different ghbps: one with high mw and low affinity and the other with lower mw and higher affinity. the high affinity ghbp was partially purified using gel filtration and aff ... | 1997 | 9255401 |
| pharmacokinetics of radioiodinated growth hormones in the turtle chrysemys dorbigni. | growth hormone binding proteins (ghbp) have been identified in the blood of many species. the aim of the present work is to study the physiological role of the ghbp in the turtle serum which we recently described. binding studies were carried out using in vivo pharmacokinetic and chromatographic techniques as well as in vitro methods. when (125)i-gh was injected in physiological concentration into chrysemys dorbigni turtles, the first step of pharmacokinetics was the binding of a significant fra ... | 1999 | 10650345 |
| third-stage larvae of the enoplid nematode dioctophyme renale (goeze, 1782) in the freshwater turtle trachemys dorbigni from southern brazil - corrigendum. | | 2015 | 25167468 |
| third-stage larvae of the enoplid nematode dioctophyme renale (goeze, 1782) in the freshwater turtle trachemys dorbigni from southern brazil. | the giant kidney worm dioctophyme renale is normally found in wild carnivores and domestic dogs, with aquatic oligochaetes acting as intermediate hosts. in the present study a prevalence of 50% of third-stage larvae of d. renale was recorded in 60 specimens of the freshwater turtle trachemys dorbigni from southern brazil. larvae were encysted in muscles, the coelomic cavity and mesentery, the serous lining of the stomach and on the surfaces of the lung, heart, liver, pancreas, spleen and intesti ... | 2015 | 24830883 |
| camallanus emydidius n. sp. (nematoda: camallanidae) in trachemys dorbigni (duméril & bibron, 1835) (testudines: emydidae) from southern brazil. | this paper describes a new species of camallanus found in the freshwater turtle trachemys dorbigni. sixty hosts collected in southern brazil were examined. all hosts (100%) were parasitized by a new species of camallanus, which was described as camallanus emydidius n. sp. the new species differs from other camallanus species of freshwater turtles mainly because of the morphology of the right spicule, the number of male precloacal and postcloacal papillae, and the presence of "mucrons" in the fem ... | 2017 | 28580298 |
| karyotypic characterization of trachemys dorbigni (testudines: emydidae) and chelonoidis (geochelone) donosobarrosi (testudines: testudinidae), two species of cryptodiran turtles from argentina. | we describe for the first time the karyotypes of two species of cryptodiran turtles from argentina, namely, trachemys dorbigni (emydidae) and chelonoidis (geochelone) donosobarrosi (testudinidae). the karyotype of t. dorbigni (2n = 50) consists of 13 pairs of macrochromosomes and 12 pairs of microchromosomes, whereas the karyotype of c. donosobarrosi (2n = 52) consists of 11 pairs of macrochromosomes and 15 pairs of microchromosomes. fluorescence in situ hybridization (fish) with a (ttaggg)n tel ... | 2009 | 19543981 |
| substance p immunoreactivity in the lumbar spinal cord of the turtle trachemys dorbigni following peripheral nerve injury. | immunoreactive substance p was investigated in turtle lumbar spinal cord after sciatic nerve transection. in control animals immunoreactive fibers were densest in synaptic field ia, where the longest axons invaded synaptic field iii. positive neuronal bodies were identified in the lateral column of the dorsal horn and substance p immunoreactive varicosities were observed in the ventral horn, in close relationship with presumed motoneurons. other varicosities appeared in the lateral and anterior ... | 2003 | 12700831 |
| distribution of glycogen phosphorylase and cytochrome oxidase in the central nervous system of the turtle trachemys dorbigni. | glycogen phosphorylase (gp) and cytochrome oxidase (co) activities were mapped histochemically in the brain of the turtle trachemys dorbigni. in the telencephalon, both activities occurred in the olfactory bulb, in all cortical areas, in the dorsal ventricular ridge, striatum, primordium hippocampi and olfactory tubercle. in the diencephalon, they were identified in some areas of the hypothalamus, and in rotundus and geniculate nuclei. both reactions were detected in the oculomotor, trochlear, m ... | 1999 | 10629952 |
| axotomy increases nadph-diaphorase activity in the dorsal root ganglia and lumbar spinal cord of the turtle trachemys dorbigni. | seven days after transection of the sciatic nerve nadph-diaphorase activity increased in the small and medium neurons of the dorsal root ganglia of the turtle. however, this increase was observed only in medium neurons for up to 90 days. at this time a bilateral increase of nadph-diaphorase staining was observed in all areas and neuronal types of the dorsal horn, and in positive motoneurons in the lumbar spinal cord, ipsilateral to the lesion. a similar increase was also demonstrable in spinal g ... | 1999 | 10347815 |
| glycemia and immunohistochemical changes in the endocrine pancreas of the turtle chrysemys dorbigni treated with streptozotocin. | the diabetogenic action of streptozotocin (sz) was investigated in the turtle chrysemys dorbigni after a 1- or 14-day fast. sz (130 or 250 mg/kg) was injected intravenously, and blood glucose and plasma insulin were measured. pancreatic endocrine cells were stained immunohistochemically by the immunoperoxidase avidin-biotin-peroxidase complex method. only 14% of the sz-treated turtles showed hyperglycemia. prolonged fasting did not increase the percentage of hyperglycemic animals. in control tur ... | 1989 | 2534475 |
| in vivo specific uptake of labeled insulin by turtle (chrysemys dorbigni) thyroid gland. | insulin labeled with 125i was injected into turtles to study its specific uptake by the thyroid gland. the gel filtration behavior of labeled material in blood and thyroid gland was determined in order to ascertain if the uptake is specific. most animals were pretreated with ki to saturate the gland with iodide. maximum specific uptake of radioactivity by the thyroid gland was only detected in animals pretreated with ki. a significant dose-related reduction (ed50 = 0.5 micrograms/kg) was observe ... | 1984 | 6383505 |
| in vivo specific uptake of labeled insulin by liver, adipose tissue, pituitary, and adrenals in the turtle chrysemys dorbigni. | | 1982 | 6751932 |
| coelioscopic-assisted prefemoral ovariosalpingectomy in a d'orbigny slider (trachemys dorbigni) using a digital otoscope. | the purpose of this study was to assess the unilateral prefemoral coelioscopic-assisted approach for ovariosalpingectomy in a d'orbigny slider (trachemys dorbigni) using a digital otoscope. twenty healthy turtles were randomly assigned to one of two groups, for right (gr; n=10) or left (gl; n=10) prefemoral access, for coelioscopic-assisted ovariosalpingectomy. anesthesia and surgery times, body weight, and ovary/oviduct weight data were recorded. anesthesia and surgery times did not differ sign ... | 2020 | 33071253 |