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estimation and economic implications of lone star tick (acari: ixodidae) infestation on weight gain of cattle, bos taurus and bos taurus x bos indicus. 19873584640
seasonal variation of ticks (ixodidae) in bos taurus x bos indicus cattle under rotational grazing in forested and deforested habitats in northwestern argentina.the seasonal occurrence of ticks infesting cattle was monitored in a bos taurus x bos indicus commercial herd fed on a combination of native grasses and annual pastures in northwestern argentina. the number of female ticks (boophilus and amblyomma) and the proportion of cattle infested with larvae and nymphs of amblyomma ticks were recorded from a sample of 15 cows in 24 visits at intervals of 21-50 days from 5 december 1986 to 24 november 1988. the cattle were maintained on native grasses in fo ...19947839563
a microsatellite within the bovine kappa-casein gene reveals a polymorphism correlating strongly with polymorphisms previously described at the protein as well as the dna level.the polymorphism of a (ta)n(ca)n repeat microsatellite present in the third intron of the bovine kappa-casein gene (cask) has been investigated. the existence of six alleles differing only in the number of dinucleotide repeats has been established. a total of 330 animals belonging to nine different pure bred bos taurus french breeds or to a cross-bred bos taurus x bos indicus population (créole) were genotyped. the distribution of the microsatellite alleles was examined and clear breed differenc ...19947985838
effect of restricted suckling of calves on the productivity of crossbred dairy cattle.an experiment on restricted suckling of crossbred dairy cows was conducted at the livestock research centre, tanga in north-east tanzania. the objective of the experiment was to evaluate the comparative productivity of bos taurus x bos indicus cows of medium and high levels of bos taurus inheritance, whose calves were either bucket-reared or suckled residual milk. lactation milk yield, length and persistency were 1563 l, 289 days, and 1.0, respectively, for the bucket-reared and 1592 l, 289 days ...200312636361
comparison of four synchronization protocols for fixed-time bovine embryo transfer in bos indicus x bos taurus recipients.the objective was to evaluate the effects of 400 iu of ecg given on days 5 or 8 of an estrus synchronization protocol with progesterone-releasing intravaginal devices (prid) and estradiol benzoate (eb), in recipients for fixed-time embryo transfer. a secondary objective was to determine the effects of injectable progesterone (given concurrent with eb treatment). three-hundred-and-four crossbred bos taurus x bos indicus beef heifers were randomly assigned to one of four treatment groups (2 x 2 fa ...200415511545
induction of ovulation with gnrh and pgf2alpha in lactating bos taurus x bos indicus cows.induction of ovulation for timed artificial insemination (tai) with the ovsynch protocol was evaluated in 49 anoestrous and lactating bos taurus x bos indicus cows. palpation per rectum and transrectal ultrasonography were used on days -30, -20, -10 and 0 (start of treatment) to confirm anoestrus but with the presence of follicles > or = 10 mm, and every other day during treatment to determine ovarian activity. cows were randomly assigned to: (1) ovsynch (n = 24; day 0, 200 microg gnrh; day 7, 1 ...200415595283
fertility on synchronized estrus in crossbred (bos taurus x bos indicus) heifers.this study was performed in western maharastra (india). the 272 crossbred heifers were randomly allocated within herds to one of the four following groups: 1) silastic coils, 10 days treatment (n = 65); 2) norgestomet implants, 10 days treatment (n = 71); 3) prostaglandin f(2alpha) two injections 11 days apart (n = 70); and 4) control (n = 66). almost all heifers in the treated groups were detected in heat during the four days following treatment (88-100%) vs 26% in the control group in the firs ...198316725856
growth hormone mrna expression in the pituitary of bos indicus and bos taurus x bos indicus crossbred young bulls treated with recombinant bovine somatotropin.the effects of breed and of recombinant bovine somatotropin (rbst) treatment on growth hormone gene expression were studied in young bulls. the experiment was completely randomized in a [2 x 2]-factorial arrangement, using two levels of rbst (0 or 250 mg/animal/14 days), and two breed groups (nelore and simmental x nelore crossbred). a cdna encoding bos indicus growth hormone was cloned and sequenced for use as a probe in northern and dot blot analyses. compared to the bos taurus structural gene ...200214963823
production and economic potentials of cattle in pasture-based systems of the western amazon region of brazil.our objectives were to evaluate strategies to improve productivity and economic returns from beef and dual-purpose cattle systems based on data collected on one dual-purpose (bos taurus x bos indicus) and two beef (nellore) cattle farms in the western amazon region of brazil. forage chemical composition and digestion rates of carbohydrate fractions of grazed brachiaria decumbens and brachiaria brizantha cv. marandu grasses and pueraria phaseoloides (tropical kudzu) legume were measured monthly d ...200314677847
semen quality and presence of cytokines in seminal fluid of bull ejaculates.it has been postulated that cytokines play an immunoregulatory role in human semen. the presence and levels of various cytokines, namely the interleukins 6, 10 (il-6 and il-10) and tumor necrosis factor alpha (tnfalpha) were investigated in the seminal plasma of six fertile crossbred bulls (bos taurus x bos indicus), using specific enzyme immunoassay. cytokine levels were related to the semen quality of these bulls. a direct significant correlation between the level of il-10 and individual sperm ...200312763168
shortening the postpartum anoestrous interval in suckled crossbred dual purpose cows using progestagen intravaginal sponges plus ecg and pgf(2alpha).one hundred and twenty-six suckled crossbred cows (bos taurus x bos indicus), with body condition score >or=3 (1-5 point scale), were employed in the present study to evaluate the effectiveness of intravaginal progestin-releasing sponges (ivs) for shortening anoestrous interval. fifty-four cows were assigned to control group. seventy-two cows were treated with ivs impregnated with 250 mg of medroxy-acetate-progesterone (map) as follows: day 0, ivs plus 5 mg of 17beta-e and 50 mg of map i.m.; day ...200918992108
replacement value of undecorticated sunflower meal as a supplement for milk production by crossbred cows and buffaloes in the northern plains of india.the replacement value of undecorticated sunflower meal (sfm) in the diets of dairy animals was assessed on-station and on-farm. eighteen primiparous crossbred (bos taurus x bos indicus) cows (350.4 +/- 8.84 kg), randomly allocated to three groups, were used in the on-station study. the animals were fed on either a conventional concentrate supplement (control) or on an experimental concentrate, in which sfm replaced 25% (sfm-25) or 50% (sfm-50) of the cp in the control supplement. green oats (ave ...200312735704
bull exposure and an increased within-day milking to suckling interval reduced postpartum anoestrus in dual purpose cows.it is hypothesized that the combined effects of suckling and milking in the dual purpose cows is one of the main suppressors of reproductive efficiency in this production system. the experiment described here examined whether managing the interval between milking and suckling could reduce the postpartum anoestrous period and whether the presence of a teaser bull could enhance the effects of these managements. the experiment involved 39 bos taurus x bos indicus cows which had an average weight of ...200212417114
geographic distribution and frequency of a taurine bos taurus and an indicine bos indicus y specific allele amongst sub-saharan african cattle breeds.we report for the first time, and for the whole of sub-saharan africa, the geographical distribution and the frequency of an indicine and a taurine y specific allele amongst african cattle breeds. a total of 984 males from 69 indigenous african populations from 22 countries were analysed at the microsatellite locus inra 124. the taurine allele is probably the oldest one on the continent. however, the taurine and the indicine alleles were present in 291 males (30%), and 693 males (70%), respectiv ...200010736042
bovine embryo transfer recipient synchronisation and management in tropical environments.numerous studies have shown that it is possible to manipulate follicular and luteal dynamics, thereby eliminating the need for oestrus detection in embryo transfer (et) programmes. fixed-time et (ftet) protocols are based on the use of gonadotrophin-releasing hormone (gnrh) and prostaglandin (pg) f or progesterone/progestogen (p4)-releasing devices and oestradiol. the ftet protocols increases the proportion of recipients transferred, and therefore pregnancy rates, compared with the use of pgf fo ...201020003847
immunization of cattle against hyalomma anatolicum anatolicum using larval antigens.development of immunity in cross-bred (bos taurus x bos indicus) calves against hyalomma anatolicum anatolicum, vector of bovine tropical theileriosis, was studied using larval antigen (ls) in freund's complete adjuvant (fca). calves immunized with ls + fca showed significant rejection of larvae (57.25 +/- 6.8) and nymphs (45.75 +/- 5.16). abnormally fed larvae (11.4 +/- 0.8) and nymphs (8.25 +/- 1.2) were also recovered from immunized calves. this abnormal feeding may possibly be attributed to ...199910641148
genome wide scan for quantitative trait loci affecting tick resistance in cattle (bos taurus x bos indicus).in tropical countries, losses caused by bovine tick rhipicephalus (boophilus) microplus infestation have a tremendous economic impact on cattle production systems. genetic variation between bos taurus and bos indicus to tick resistance and molecular biology tools might allow for the identification of molecular markers linked to resistance traits that could be used as an auxiliary tool in selection programs. the objective of this work was to identify qtl associated with tick resistance/susceptibi ...201020433753
moxidectin pour-on for control of natural populations of the cattle tick boophilus microplus (acarina: ixodidae).fifty bos taurus x bos indicus heifers naturally infested with boophilus microplus ticks were divided into two groups of 25 heifers each. individuals of one group were treated with moxidectin 0.5% pour-on at a dosage of 500 microg of moxidectin/kg body weight and heifers from the other group remained as untreated controls. an efficacy higher than 95% was found on days 7-21 after treatment by using female ticks 4.5-8.0 mm long as the main infestation parameter. a lower, but significant efficacy ( ...200010622616
evaluation of line and breed of cytoplasm effects on performance of purebred brangus cattle.substantial differences between reciprocally crossed bos taurus x bos indicus calves for birth, weaning, and yearling weights have been reported. to determine whether cytoplasmic inheritance is responsible for a portion of these differences, field records for birth and weaning weight (n = 7,353) and postweaning average daily gain (n = 2,746) from registered brangus calves were analyzed. an animal model that included maternal effects was fit for each trait. breed of cytoplasmic origin was fit as ...19947730171
treatment of experimentally induced theileria annulata infection in cross-bred calves with buparvaquone.twenty cross-bred (bos taurus x bos indicus) calves, 7-21 days old, were infected by a ground-up tick supernate of hyalomma anatolicum anatolicum infected with the hisar isolate of theileria annulata. six calves acted as untreated controls and they all died of theileriosis within 17 days of infection. the remaining 14 calves were divided into group a and b, each consisting of seven calves. all the calves of groups a and b were treated intramuscularly with buparvaquone (bw 720c) on day 11 post-in ...19883369076
immunization of neonatal bovines against theileria annulata by an infection and treatment method.sixty three cross-bred (bos taurus x bos indicus) 4-5-day-old calves were divided into 16 groups (a-p). each calf in groups a and b was given ground-up-tick supernate prepared from theileria annulata-infected or non-infected hyalomma anatolicum anatolicum (guts) equivalent to 5 ticks (50 infected acini). groups d and e received infected guts equivalent to 2 ticks (20 infected acini) and groups g and h were given infected guts equivalent to 1 tick (10 infected acini). each calf in groups j, k and ...19873113041
joint report of the third international bovine lymphocyte antigen (bola) workshop, helsinki, finland, 27 july 1986.two hundred and eighty-two alloantisera were submitted by 20 participating laboratories from 13 countries and tested against lymphocytes of 1298 cattle. the cell panel consisted of samples from 38 bos taurus breeds, 11 bos taurus crossbreeds, 4 bos indicus breeds, 6 bos taurus x bos indicus, and a variety of other crossbred populations. using a standardized lymphocytotoxicity test, all 17 previously identified bola specificities were confirmed. the workshop produced agreement on 16 new lymphocyt ...19892729671
immunization of cattle (bos indicus x bos taurus) against hyalomma anatolicum anatolicum using antigens derived from tick salivary gland extracts.studies were conducted on 41 cross-bred (bos taurus x bos indicus) calves which were less than 1 year old. three types of salivary gland antigens (sg ag) viz. sg ag-i (whole), sg ag-ii (supernatant) and sg ag-iii (sediment), prepared from the ixodid tick, hyalomma anatolicum anatolicum, were used for immunizing these calves. the calves were divided into five groups. the first three groups were immunized with sg ag-i, -ii and -iii with freund's complete adjuvant (fca), respectively. the fourth gr ...19902276870
attempted immunisation of crossbred calves (bos taurus x bos indicus) by repeated natural attachment of ticks hyalomma anatolicum anatolicum koch (1844).crossbred calves (bos taurus x bos indicus) were repeatedly infested with the ixodid tick, hyalomma anatolicum anatolicum. the parameters of engorgement, pre-oviposition period, oviposition period, engorged weight, egg batch weight, and per cent hatch of eggs all showed statistically significant differences between first to tenth infestation. only the time to engorgement did not change. it is concluded that repeated infestations of this tick stimulate acquired resistance.19911796526
geostatistical analysis of biomarkers of genotoxicity in cattle, bos taurus and bos taurus x bos indicus, sentinels near industrial facilities.this study, performed at the behest of ranchers living and working down-prevailing wind from industrial facilities located in calhoun county, texas investigated locational risks to ecosystem health associated with proximity to specific industrial complexes. concerns expressed were for potential genotoxicity in cattle resulting from the release of complex chemical mixtures. the comet assay and flow cytometric evaluation of variations in dna content were utilized to evaluate dna damage. bayesian g ...200918763035
promoter region of the bovine growth hormone receptor gene: single nucleotide polymorphism discovery in cattle and association with performance in brangus bulls.expression of the gh receptor (ghr) gene and its binding with gh is essential for growth and fat metabolism. a gt microsatellite exists in the promoter of bovine ghr segregating short (11 bp) and long (16 to 20 bp) allele sequences. to detect snp and complete an association study of genotype to phenotype, we resequenced a 1,195-bp fragment of dna including the gt microsatellite and exon 1a. resequencing was completed in 48 familialy unrelated holstein, jersey, brown swiss, simmental, angus, brah ...200818676722
effects of polymorphisms of lhr and fshr genes on sexual precocity in a bos taurus x bos indicus beef composite population.the purpose of the present research was to investigate the effects of polymorphisms of luteinizing hormone receptor (lhr) and follicle-stimulating hormone receptor (fshr) genes, evaluated by polymerase chain reaction-restriction fragment length polymorphism in european-zebu composite beef heifers from six different breed compositions. the polymorphism site analysis from digestion with hhai and alui restriction endonucleases allowed the genotype identification for lhr (tt, ct and cc) and fshr (gg ...200818393228
genetic parameters for growth traits of a brazilian bos taurus x bos indicus beef composite.the genetic analysis of composite data is very complicated, mainly because it is necessary to adjust data to the effects of heterosis and breed complementarity, and because there is usually considerable confounding of these data with several other effects, such as contemporary group effects, breed composition of the animal and maternal breed composition, among others. data on birth weight (n = 151,083), weaning weight adjusted to 205 days (n = 137,257), yearling weight adjusted to 390 days (n = ...200718273812
effect of coumaphos on cholinesterase activity, hematology, and biochemical blood parameters of bovines in tropical regions of mexico.to assess the effect of coumaphos [o-(3-chloro-4-methyl-2-oxo-2h-1-benzopyran-7-yl) o,o-diethyl phosphorothioate] exposure on physiological responses during bovine production, acetylcolinesterase (ache) and butyrylcholinesterase (buche) activities were measured in whole blood, erythrocytes, and plasma of healthy male steers (bos taurus x bos indicus) sprayed with coumaphos at a non-lethal dose of 1 mg kg(- 1) body weight per day once every 14 (in vivo group) or 21 days (southern and central grou ...200717474014
association analysis of adiponectin and somatostatin polymorphisms on bta1 with growth and carcass traits in angus cattle.this study tested positional candidate genes adiponectin (adipoq) and somatostatin (sst) for effects on carcass traits in a commercially relevant cattle population. both genes are located within a region of bta1 previously reported to harbour quantitative trait loci (qtl) that affect marbling, quality grade, yield grade, ribeye area and weaning weight in bos taurus x bos indicus crosses. except for the first intron of adipoq, both genes, including over 2 kb upstream of the promoters, were sequen ...200617121600
evaluation of dna polymorphisms involving growth hormone relative to growth and carcass characteristics in brahman steers.associations of dna polymorphisms in growth hormone (gh) relative to growth and carcass characteristics in growing brahman steers (n = 324 from 68 sires) were evaluated. polymorphisms were an msp-i rflp and a leucine/valine snp in the gh gene as well as a hinf-i rflp and a histidine/arginine snp in transcriptional regulators of the gh gene, pit-1 and prop-1. genotypic frequencies of the gh snp, pit-1 rflp, and prop-1 snp were greater than 88% for one of the bi-allelic homozygous genotypes. genot ...200617117358
genetic effects on preweaning weight gain of nelore-hereford calves according to different models and estimation methods.additive and nonadditive genetic effects on preweaning weight gain (pwg) of a commercial crossbred population were estimated using different genetic models and estimation methods. the data set consisted of 103,445 records on purebred and crossbred nelore-hereford calves raised under pasture conditions on farms located in south, southeast, and middle west brazilian regions. in addition to breed additive and dominance effects, the models including different epistasis covariables were tested. model ...200617032785
artificial insemination of subtropical commercial beef cattle following synchronization with cloprostenol (ici 80996): i. fertility.two trials were conducted over a two-year period with 519 cycling bos taurus x bos indicus heifers and cows. the objectives of these trials were: 1) to compare fertility of artificial insemination at the cloprostenol-induced estrus and the naturally occurring estrus, 2) to evaluate the fertility of artificial insemination at a predetermined time (timed ai) following an estrous synchronization regime with cloprostenol (clp) and 3) to define the optimum interval from a second clp treatment for tim ...198016725522
babesia spp. infection in boophilus microplus engorged females and eggs in sao paulo state, brazil.babesia spp. infections were investigated in bos taurus x bos indicus dairy cows and calves and in boophilus microplus engorged female ticks and eggs. blood samples and engorged female ticks were collected from 25 cows and 27 calves. babesia spp. was detected in ticks by microscopic examination of hemolymph of engorged female and by squashes of egg samples. cattle infection was investigated in blood thin smears and by dna amplification methods (pcr and nested pcr), using specific primers for bab ...200515893070
evaluation of closed adult nucleus multiple ovulation and embryo transfer and conventional progeny testing breeding schemes for milk production in tropical crossbred cattle.the potential benefits of closed adult nucleus multiple ovulation and embryo transfer (moet) and conventional progeny testing (cns) schemes, and the logistics of their integration into large-scale continuous production of crossbred cattle were studied by deterministic simulation. the latter was based on f1 (bos taurus x bos indicus) production using ai or natural mating and moet, and continuous f2 production by mating of f1 animals. the gene flow and the cumulative discounted expressions (cdes) ...200515778328
cow/calf preweaning efficiency of nellore and bos taurus x bos indicus crosses.the objectives of this study were to determine if percentage bos taurus (0 or 50%) of the cow had an effect on me requirements and milk production, and to compare cow/calf efficiency among 3 mating systems. metabolizable energy requirements were estimated during a feeding trial that encompassed a gestation and lactation feeding trial for each of 2 groups of cows. cows were 0 or 50% bos taurus (100 or 50% nellore) breed type: nellore cows (nl; n = 10) mated to nellore bulls, nl cows (n = 9) mated ...200918952743
differential methylation status of igf2-h19 locus does not affect the fertility of crossbred bulls but some of the ctcf binding sites could be potentially important.associations between abnormal methylation of spermatozoan dna with male infertility have been sought in recent years to identify a molecular explanation of differential spermatozoan function. the present work was undertaken to investigate the methylation profile of differentially methylated regions (dmrs) in the igf2-h19 locus of bos taurus x bos indicus crossbred bull spermatozoa. bulls having more than at least 100 insemination records over a period of 12 years were classified into two groups ...201424436206
effect of ruminally protected methionine on the productive and reproductive performance of grazing bos indicus heifers raised in the humid tropics of costa rica.with the objective of evaluating the effect of methionine supplementation prior to a breeding program, thirty one heifers (bos taurus x bos indicus) were used averaging 386 +/- 29 days of age and a mean body weight of 402.6 +/- 28 kg. fifteen of the animals received a supplement (sg) during 45 days with molasses-urea mixture (2 kg molasses + 407 g urea/head/day), plus 10 g of ruminally protected methionine. the other sixteen heifers did not receive supplement (cg). fecal and pastures samples wer ...200818975132
ascorbic acid, catalase and chlorpromazine reduce cryopreservation-induced damages to crossbred bull spermatozoa.the present study evaluated the effectiveness of ascorbic acid, catalase, chlorpromazine and their combinations in reducing the cryodamages to crossbred bull (bos taurus x bos indicus) spermatozoa. a total of 32 ejaculates (eight each from four bulls) were diluted in tris-citric acid-fructose-egg yolk-glycerol extender. each ejaculate was split into six parts (five treatment and one control). treatment groups included 10 mm ascorbic acid, 0.1 mm chlorpromazine, 200 iu/ml catalase, 10 mm ascorbic ...201019032433
non-additive genetic effects on weights and performance of a brazilian bos taurus x bos indicus beef composite.the aim of the present study was to evaluate the heterosis effects on weaning weight at 205 days (ww, n = 146,464), yearling weight at 390 days (yw, n = 69,315) and weight gain from weaning to yearling (wg, n = 59,307) in composite beef cattle. the fixed models were: rm, which included contemporary groups, class of age of dam, outcrossing percentages for direct and maternal effects, and additive direct and maternal (am) breed effects; r, rm model, minus am breed effects, and h, rm model, minus a ...200819048494
gastrointestinal nematode infection in beef cattle of different genetic groups in brazil.resistance to natural infection by gastrointestinal nematodes was compared in 67 female calves of the following genetic groups: nelore (nx); 1/2 senepol+1/2 nelore (sn); and 1/2 aberdeen angus+1/2 nelore (an). the nx (n=26), sn (n=23) and an (n=18) animals were monitored for 14 months, during which they remained without treatment, allowed to graze in a tropical environment. eggs per gram of feces (epg), coprocultures and packed cell volume (pcv) were carried out monthly. no significant effects o ...200919828253
effect of supplementation with corn oil on postpartum ovarian activity, pregnancy rate, and serum concentration of progesterone and lipid metabolites in f1 (bos taurus x bos indicus) cows.the aim was to evaluate the effect of corn oil supplementation during postpartum anoestrus on ovarian activity, pregnancy rate, progesterone (p(4)), and lipid metabolites (cholesterol, cho; low and high density lipoproteins; ldl and hdl, respectively) concentrations in blood of f(1) (bos taurus x bos indicus) grazing cows. cows were randomly assigned to an experimental group, fed with a supplement containing 4% corn oil on dry matter basis (og, n = 11), and a control group with the same suppleme ...201020379774
genetic association between bovine nod2 polymorphisms and infection by mycobacterium avium subsp. paratuberculosis in holstein-friesian cattle.nucleotide-binding oligomerization domain 2 (nod2) has been reported to be a candidate gene for mycobacterium avium subsp. paratuberculosis (map) infection in a bos taurus × bos indicus mixed breed based on a genetic association with the c.2197t>c single nucleotide polymorphism (snp). nevertheless, this snp has also been reported to be monomorphic in the b. taurus species. in the present work, 18 snps spanning the bovine nod2 gene have been analysed in a genetic association study of two independ ...201020477790
comparative analysis of mafriwal (bos taurus × bos indicus) and kedah kelantan (bos indicus) sperm proteome identifies sperm proteins potentially responsible for higher fertility in a tropical climate.the fertility of zebu cattle (bos indicus) is higher than that of the european purebred (bos taurus) and crossbred (bos taurus × bos indicus) cattle in tropical areas. to identify proteins related to the higher thermo-tolerance and fertility of zebu cattle, this study was undertaken to identify differences in sperm proteome between the high fertile malaysian indigenous zebu cattle (kedah kelantan) and the sub-fertile crossbred cattle (mafriwal). frozen semen from three high performance bulls fro ...201323903046
genetic susceptibility to infectious disease in east african shorthorn zebu: a genome-wide analysis of the effect of heterozygosity and exotic introgression.positive multi-locus heterozygosity-fitness correlations have been observed in a number of natural populations. they have been explained by the correlation between heterozygosity and inbreeding, and the negative effect of inbreeding on fitness (inbreeding depression). exotic introgression in a locally adapted population has also been found to reduce fitness (outbreeding depression) through the breaking-up of co-adapted genes, or the introduction of non-locally adapted gene variants. in this stud ...201324209611
gamete therapeutics: recombinant protein adsorption by sperm for increasing fertility via artificial insemination.a decrease in fertility can have a negative economic impact, both locally and over a broader geographical scope, and this is especially the case with regard to the cattle industry. therefore, much interest exists in evaluating proteins that might be able to increase the fertility of sperm. heparin binding proteins (hbps), specifically the fertility associated antigen (faa) and the type-2 tissue inhibitor of metalloproteinase (timp-2), act to favor the capacitation and acrosome reaction and perha ...201323762288
absolute copy number differences of y chromosomal genes between crossbred (bos taurus × bos indicus) and indicine bulls.the y chromosome in mammal is paternally inherited and harbors genes related to male fertility and spermatogenesis. the unique intra-chromosomal recombination pattern of y chromosome and morphological difference of this chromosome between bos taurus and bos indicus make it an ideal model for studying structural variation, especially in crossbred (bos taurus × bos indicus) bulls. copy number variation (cnv) is a type of genomic structural variation that gives information complementary to snp data ...201323556478
association of single nucleotide polymorphisms in capn1, cast and mb genes with meat color of brahman and crossbreed cattle.the objective of this research was to determine the association of snps in the candidate genes calpain (capn1), calpastatin (cast) and myoglobin (mb) with colorimetric parameters (l *, a *, b *, c *, hue) in a f1 population (n = 164) obtained from crossing bos taurus × bos indicus and bos indicus × bos indicus. snps were analyzed using pcr-rflp and sscp. colorimetric measurements were performed in the muscles longissimus thoracis et lumborum (ltl) and semitendinosus (st) at 7, 14 and 21 days pos ...201626946475
novel snp identification in exon 3 of hsp90aa1 gene and their association with heat tolerance traits in karan fries (bos taurus × bos indicus) cows under tropical climatic condition.heat shock proteins (hsps) act as molecular chaperones those are preferentially transcribed in respose to heat stress and the polymorphism in hsp genes associated with heat tolerance traits in cows. hsp90aa1 gene has been mapped on bos taurus autosome 21 (bta-21) and spans nearly 5368 bp comprising of 11 exons out of which the first exon does not translate. the present study was done on karan fries (5/8 hf × 3/8 tharparkar) cows reared in tropical climate with the objectives of identifying singl ...201626898694
energy utilization, nitrogen balance and microbial protein supply in cattle fed pennisetum purpureum and condensed tannins.the aim of the experiment was to assess the effect of condensed tannins (ct) on feed intake, dry matter digestibility, nitrogen balance, supply of microbial protein to the small intestine and energy utilization in cattle fed a basal ration of pennisetum purpureum grass. five heifers (bos taurus × bos indicus) with an average live weight of 295 ± 19 kg were allotted to five treatments consisting of increasing levels of ct (0, 1, 2, 3 and 4% ct/kg dm) in a 5 × 5 latin square design. dry matter int ...201726849400
prediction of intake in growing dairy heifers under tropical conditions.a meta-analysis was conducted to develop models of the prediction of dry matter intake (dmi) in growing dairy heifers [postweaning to 390 kg of body weight (bw)] under tropical conditions. the adequacy of the models was assessed in a comparison with the 4 us models currently used to predict dmi [quigley; national research council; and 2 hoffman models]. the data set was created using 95 treatment means from 28 studies published in journals. the data set (studies) was randomly divided into 2 data ...201626709180
stocking rate and monensin supplemental level effects on growth performance of beef cattle consuming warm-season grasses.the objective of this study was to evaluate the effects of monensin supplementation on animals receiving warm-season grass with limited supplementation. in exp. 1, treatments were a factorial combination of 2 stocking rates (1.2 and 1.7 animal unit [au] [500 kg bw]/ha) and supplementation with monensin (200 mg/d) or control (no monensin) distributed in a complete randomized design with 3 replicates. thirty angus × brahman crossbred heifers (bos taurus × bos indicus) with initial bw of 343 ± 8 kg ...201526440034
ovarian activity and estrus behavior in early postpartum cows grazing leucaena leucocephala in the tropics.the legume leucaena leucocephala (leucaena) is widely used to supplement forage in silvopastoral livestock systems in latin america. little is known about its possible effects on the cow reproductive dynamic. the aim was to evaluate the effect of leucaena foliage intake on re-establishment of ovarian activity and estrus behavior in early postpartum (7-90 days) cows. twenty-four multiparous bos taurus × bos indicus cows were divided into two homogenous groups and assigned to one of two treatments ...201526210396
comparative proteomic analysis of taurine, indicine, and crossbred (bos taurus × bos indicus) bull spermatozoa for identification of proteins related to sperm malfunctions and subfertility in crossbred bulls.subfertility is one of the most common problems observed among taurine × indicine crossbred bulls in tropical countries; however, the etiology remain unknown in most of the cases. in present study, we compared the proteomic profile of spermatozoa from crossbred bulls (bos taurus × bos indicus) against their purebred parent lines (holstein friesian [taurine] and tharparkar [indicine]) to find out alteration in expressions of proteins, if any. the proteomic profiles of freshly ejaculated spermatoz ...201526033646
copy number differences of y chromosomal genes between superior and inferior quality semen producing crossbred (bos taurus × bos indicus) bulls.the removal of crossbred bulls from semen collection programs due to the production of poor quality semen causes substantial monetary losses to the dairy industry. seminal quality, a quantitative trait, is greatly influenced by genome level variations. deletion and/or duplication of y chromosomal genes and subsequent changes in gene copy number have a major role in determining spermatogenic efficiency and, therefore, seminal quality. in this study, copy numbers of three y chromosomal genes tspy, ...201525153458
adaptive admixture in the west african bovine hybrid zone: insight from the borgou population.understanding the adaptive response to environmental fluctuations represents a central issue in evolutionary biology. population admixture between divergent ancestries has often been considered as an efficient short-term adaptation strategy. cattle populations from the west african bos taurus × bos indicus hybrid zone represent a valuable resource to characterize the effect of such adaptive admixture at the genome level. we here provide a detailed assessment of the global and local genome ancest ...201424888437
a disparate trace element metabolism in zebu (bos indicus) and crossbred (bos indicus × bos taurus) cattle in response to a copper-deficient diet.copper deficiency is a commonly diagnosed problem in cattle around the globe. in jimma, ethiopia, 8 zebu (bos indicus) and 8 zebu ×: holstein friesian cross (bos taurus ×: bos indicus) heifers were used in an 11-wk study to investigate breed type differences and effects of cu deficiency on concentrations of trace elements in plasma and edible tissues as well as mrna expression of cu-related genes. heifers were fed a grass diet (6.4 ± 0.2 [sem] mg cu/kg dm) supplemented with 1 mg mo/kg dm in wk 1 ...201424829510
polymorphism of growth hormone gene and its association with seminal and sexual behavioral traits in crossbred cattle.the decline in the male reproductive ability in terms of sexual behavior and seminal traits might lead to nonavailability of required number of bulls in a progeny testing program. the present study was conducted in 493 crossbred cattle (bos taurus × bos indicus) bulls to study polymorphisms of growth hormone (gh) gene and its association with seminal and sexual behavioral characteristics. a 428-base pair fragment of gh gene spanning over the fourth exon, fourth intron, and fifth exon was amplifi ...201424315684
subspecies differences in early fetal development and plasma pregnancy-associated glycoprotein concentrations in cattle.inclusion of bos indicus genetics improves production traits of cattle maintained in hot climates. limited information exists detailing pregnancy-specific events as influenced by variable amounts of bos indicus genetics. three experiments were completed to examine the effect of bos taurus and bos indicus genotypes on fetal size and plasma pregnancy-associated glycoprotein (pag) concentrations. in all experiments, cows were bred by ai after synchronization of ovulation. fetal measurements were co ...201323881679
genetic (co)variance for sire fertility estimated by additive, non-additive and longitudinal models in holstein-zebu cross-bred cows.for this study, we used the results of sire fertility (sf) measured on a monthly basis by the ratio between the number of pregnant cows and the number of inseminations, from a total of 905 140 inseminations carried out in cuba between 1994 and 2003. these artificial inseminations were made using 815 sires in 3249 herds throughout the country, and were analysed using additive, non-additive linear models and a random regression model (rrm). the additive genetic (add), heterosis (het) and recombina ...201323228636
molecular characterization and snp identification in hspb6 gene in karan fries (bos taurus x bos indicus) cattle.heat shock proteins (hsps) act as molecular chaperones which are preferentially transcribed in response to severe perturbations of the cellular homeostasis such as heat stress. the present study was undertaken for molecular characterization and detection of genetic polymorphisms of hspb6 gene in 100 karan fries cattle. hspb6 gene was mapped on bos taurus autosome 18 (bta 18), comprising three exons and two introns. four sets of primers for hspb6 gene were designed using primer3 software (version ...201728364265
profiling of sperm gene transcripts in crossbred (bos taurus x bos indicus) bulls.crossbred cattle in some sectors of the world have a significant role in enhancing milk production thereby enhancing the per capita milk availability as a human food source. however, there are certain constraints associated with crossbred animals, such as disease susceptibility, increased reproductive problems, repeat breeding and poor seminal quality. the semen of crossbred bulls has a poor freezing capacity, increased cryo-damage, poor mass cell motility, greater percentages of dead/abnormal s ...201727993430
long-term effects of good handling practices during the pre-weaning period of crossbred dairy heifer calves.the aim of this study was to determine whether applying good practices of handling during the pre-weaning period have long-term effects on behavioral and physiological indicators, health status, and average daily gain (adg) of crossbred bos taurus × bos indicus heifer calves. during the pre-weaning period, 98 crossbred of holstein × gir heifer calves were allotted into three treatments: (1) good practices of handling + brushing (gpb; n = 25), (2) good practices of handling (gp; n = 25), and (3) ...201727785765
trimethylation of histone 3 at lysine 4 in cryopreserved bovine embryos produced in vivo with sexed semen.the production rates of viable embryos using sexed semen through the conventional methodologies of multiple ovulation and embryo transfer are generally not satisfactory. however, the cryopreservation of these embryos is considered efficient. knowledge of epigenetics can provide new tools or allow for adapting new protocols that could enhance the efficiency of reproductive biotechnologies. the aim of this study was to characterize the pattern of trimethylation of histone 3 at lysine 4 (h3k4me3) i ...201627515411
equine chorionic gonadotropin increases fertility of grazing dairy cows that receive fixed-time artificial insemination in the early but not later postpartum period.this study evaluated effects of equine chorionic gonadotropin (ecg) on fertility of 679 crossbred (bos taurus x bos indicus) lactating grazing cows synchronized for fixed-time ai (ftai). at a random day of the estrous cycle cows received an intravaginal progesterone (p4) implant, 2 mg estradiol benzoate (eb) and 100 μg gonadorelin (d0-am). on d7-am, cows received 0.5 mg sodium cloprostenol and were randomly assigned into two treatments: ecg (n = 340; 400 iu ecg on d7), or control (n = 339; no ec ...201728601153
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