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sterility in hybrid cattle. i. distribution of constitutive heterochromatin and nucleolus organizer regions in somatic and meiotic chromosomes.the distribution of constitutive heterochromatin and nucleolus organizer regions (nor's) in somatic as well as in meiotic chromosomes of bos taurus, bos banteng, bison bison, and their hybrids are analyzed. c-bands are present in the centromeric regions of every autosome. the x chromosome does not show a distinct c-band in the centromeric region, whereas the y chromosome contains an appreciable amount of c-band material. in somatic metaphases, nor's are present on the telomeric ends of five pair ...197988307
synaptonemal complex analysis of hybrid cattle. ii. bos indicus x bos taurus f1 and backcross hybrids.the levels of meiotic chromosome pairing abnormalities observed in six australian f1 bos indicus x bos taurus cattle crosses (mean = 23%) were significantly higher than those of the full-blood breeds (9%). the abnormal configurations in the f1 hybrids included partial pairing failure, multivalents, interlocks, and inversion pairing. abnormal configurations were also present, but at lower frequency, in backcross hybrid bulls. the main types of abnormal configurations and the levels of xy-autosoma ...19912055447
synaptonemal complex analysis of hybrid cattle. iii. meiotic pairing mechanisms in f1 brahman x hereford hybrids.the mechanisms of homologous chromosome pairing were studied in synaptonemal complex (sc) spreads of f1 brahman (bos indicus) x hereford (bos taurus) cattle. the most common sc abnormalities were bivalents with partial pairing failure and interlocks. while c-band polymorphisms could underlie most of the sc abnormalities observed in the full-blood cattle, other causes seem also to be contributing in the hybrids. the pattern of the abnormalities indicates that genic differences between the species ...19912055448
immunization of cattle (bos indicus x bos taurus) against hyalomma anatolicum anatolicum using antigens derived from tick salivary gland extracts.studies were conducted on 41 cross-bred (bos taurus x bos indicus) calves which were less than 1 year old. three types of salivary gland antigens (sg ag) viz. sg ag-i (whole), sg ag-ii (supernatant) and sg ag-iii (sediment), prepared from the ixodid tick, hyalomma anatolicum anatolicum, were used for immunizing these calves. the calves were divided into five groups. the first three groups were immunized with sg ag-i, -ii and -iii with freund's complete adjuvant (fca), respectively. the fourth gr ...19902276870
synaptonemal complex analysis of hybrid cattle. i. pachytene substaging and the normal full bloods.meiotic chromosome pairing abnormalities in full-blood and hybrid bos taurus and b. indicus cattle have been surveyed by electron microscopy of pachytene synaptonemal complex spreads. in this paper the full-blood spreads are described in detail, including the use of xy and nucleolar morphology and other measured parameters for pachytene substaging. pairing abnormalities were observed in up to 9% of the full-blood spreads. most of these pairing abnormalities (83%) occurred in early-pachytene spre ...19892620818
relationship between the resistance of crossbred cattle to ticks, boophilus microplus (canestrini, 1887) and hyalomma anatolicum anatolicum (koch, 1844).studies were undertaken to determine the effect of repeated pure infestations with boophilus microplus on susceptibility to subsequent pure infestations with hyalomma anatolicum anatolicum, and the effects of pure infestations with both species of tick on susceptibility to a series of mixed infestations. crossbred (bos indicus x bos taurus) calves were infested with boophilus microplus (17 times), h. a. anatolicum (four times), followed by five mixed infestations of b. microplus and h. a. anatol ...19892763448
the crossbred sire: experimental results for cattle.results comparing crossbred bulls relative to straightbred bulls for various reproduction traits (such as puberty, sperm concentration, pregnancy rate and weaning rate) and progeny growth traits (birth and weaning weight) were summarized separately for bos taurus x bos taurus (bt x bt) and bos indicus x bos taurus (bi x bt) crosses. the bt x bt and bi x bt crossbred bulls were 1.8 and 5.0% younger, respectively, at puberty than straightbred bulls. also, sperm concentration of bt x bt and bi x bt ...19873301778
acquisition and expression of resistance by bos indicus and bos indicus x bos taurus calves to amblyomma americanum infestation.purebred and crossbred bos indicus calves were infested 1, 2, or 3 times with 10 female and 5 male amblyomma americanum. resistance was acquired by both the purebred and the crossbred calves after 1 infestation and resulted in statistically significant decreases in the percentages of females that engorged, the mean weights of engorged females, and the mean weights of egg masses. comparisons between breeds of the percent of female ticks that engorged during the first and second infestations indic ...19853998956
[carbohydrate metabolic enzyme activity and the glycogen content in the muscles of purebred and hybrid cattle]. 19734364296
reproductive efficiency of heifers in the new guinea highlands.bos indicus x bos taurus heifers were exposed to bulls at four different liveweights. the age and weight at first and second conception and first calf growth rates were studied to examine the effects of uncontrolled mating prior to or at a target mating weight of 295 kg. initial conception rate was not affected although lighter conception weights were recorded for three groups of heifers that were served when below the target weight. however, at subsequent calving only the two heavier groups cal ...19836868133
duration of the seminiferous epithelial cycle in hybrid bos indicus x bos taurus bulls.the hybrids were carrying 50% (n = 4) or 75% (n = 4) zebu (brahman) blood. the spermatogenic cells were labelled in vivo with [3h]thymidine. the relative frequencies of the seminiferous epithelial cycle stages, determined from examination of 6350 tubule cross-sections, did not differ between the 2 hybrid types examined, between regions of the testis or between testes within animals. frequencies were generally similar to those for bos taurus bulls except that the frequency of stage 4 tubules was ...19827175828
a survey of cattle producers in the boophilus microplus endemic area of queensland to determine attitudes to the control of and vaccination against tick fever.a survey by mail was used to determine the views of beef producers in the boophilus microplus endemic area of queensland on the control of and vaccination against tick fever. data from 448 questionnaires were analysed, representing 2.7% of beef producers in the survey area. producers considered buffalo fly (haematobia irritans exigua) infestation as the most important problem whereas tick fever ranked sixth overall. private veterinarians were regarded as the most important source of information ...19957611988
enhanced immunity to hyalomma anatolicum anatolicum ticks in cross-bred (bos indicus x bos taurus) calves using ascaris extract immunomodulator with the tick salivary gland extract antigens.development of acquired immunity in cross-bred (bos indicus x bos taurus) calves against hyalomma anatolicum anatolicum, a tick vector of bovine tropical theileriosis was studied using ascaris extract (ae), an immunomodulator of ige responses, along with the tick salivary gland extract (sge) antigens in freund's incomplete adjuvant (fia) emulsion. calves immunised with sge + fia showed significant rejection (47.4 +/- 2.8%) of larvae, whereas only marginal rejection (12.47 +/- 1.5%) of nymphs was ...19968792570
immunological cross-reactivity between salivary gland proteins of hyalomma anatolicum anatolicum and boophilus microplus ticks.immunological cross-reactivity was established between salivary gland extract antigens of hyalomma anatolicum anatolicum (h.a.a) and boophilus microplus (b.m.), using native-polyacrylamide gel electrophoresis (native-page), immunoblotting, dot-enzyme immunoassay (dot-eia) and in vivo intra-dermal skin test revealing immediate type hypersensitivity swelling reaction. native-page revealed six proteins in common of molecular weights of 60, 66, 148, 264, 300 and > 300 kda in the salivary gland extra ...19968792571
cattle theileriosis in china.amongst the piroplasmoses of livestock in china, the commonest theilerioses are caused by theileria annulata, t. sergenti and t. mutans. research carried out over many years has shown these to be distributed mainly in northern china but they also occur in southern china. t. annulata and t. sergenti are the most troublesome, affecting large numbers of cattle, especially those of exotic breeds. the incident rate and numbers of fatalities vary widely between areas and breeds of cattle. t. annulata ...19979512736
efficacy of dicyclanil in the prevention of screwworm infestation (cochliomyia hominivorax) in cattle castration wounds.a field trial was carried out during summer 1996 in a commercial beef cattle farm located in the central area of argentina (30 degrees 16's, 60 degrees 30'w) to evaluate the efficacy of a new insect growth regulator material (dicyclanil) in the prevention of myiosis caused by screwworm (cochliomyia hominivorax) larvae. forty bull calves (bos indicus x bos taurus), 5 to 6 months old were allocated by ranked paris to a control (g.1) and a treated group (g.2) of the same number of animals. on day 0 ...19989615956
immunization of cattle with nymphal hyalomma anatolicum anatolicum extracts: effects on tick biology.antigens derived from partially engorged nymphs of hyalomma anatolicum anatolicum were used in immunizing crossbred (bos indicus x bos taurus) cattle against larval, nymphal and adult h. a. anatolicum and h. dromedarii. the cattle were either infected with theileria annulata at low parasitaemia or were uninfected. whole nymphal extract (wne), nymphal membrane antigens (nma) and nymphal soluble antigens (nsa) were used for immunization. the group immunized with wne showed significant and better r ...19989719836
spermiogram and sperm reserves in hybrid bos indicus x bos taurus bulls after scrotal insulation.scrotal insulation for 48 h raised subcutaneous scrotal temperature by 4 degrees c in hybrid bos indicus x bos taurus bulls. the incidence of decapitated spermatozoa in the ejaculate increased significantly between 6 and 14 days and that of protoplasmic droplets and tail abnormalities between 20 and 23 days after insulation, respectively. simultaneously, the percentages of spermatozoa with lost and damaged acrosomes increased significantly 12-17 days after insulation. at slaughter 23 days after ...19836631831
progestogen and prostaglandin-combined treatments for synchronization of oestrus in post partum crossbred (bos indicus x bos taurus) or zebu cows. 19846593773
composition and yield of milk from beef-type bos taurus and bos indicus x bos taurus dams.yield, butterfat, protein, lactose and solids-not-fat of milk from mature dams (n = 128) representing eight bos taurus and bos indicus x bos taurus breed types were evaluated approximately 60, 105 and 150 d postpartum. breed type was a significant source of variation in milk yield at each stage of lactation. average 24-h milk yields (kg) were: hereford, 7.3; red poll, 9.1; hereford x red poll, 9.1; red poll x hereford, 9.1; angus x hereford, 8.6; angus x charolais, 9.3; brahman x hereford, 7.3 a ...19873558145
maternal characteristics of young dams representing bos taurus and bos indicus x bos taurus breed types.reproductive traits and preweaning growth of progeny from young hereford, red poll, hereford x red poll, red poll x hereford, angus x hereford, angus x charolais, brahman x hereford and brahman x angus dams were evaluated. first-calf heifers were mated with red angus bulls; santa gertrudis sires were used for each cow's second and third breeding season. herefords, red polls and hereford-red poll crosses were below average in percentage of calves weaned, whereas angus-sired and brahman-sired dams ...19883397342
circumocular pigmentation and incidence of ocular squamous cell tumors in bos taurus and bos indicus x bos taurus cattle.amount of circumocular pigmentation and incidence of ocular squamous cell tumors were evaluated in dams representing diverse cattle breed types. each dam was examined twice annually during a specific stage in the life cycle (3.5 to 9.5 years old). overall, 28.3% of dams in the herd developed at least 1 ocular squamous cell tumor. breed groups differed (p less than 0.01) in amount of circumocular pigmentation and incidence of tumors. results indicate that losses from ocular squamous cell tumors i ...19902347752
life-cycle evaluation of bos taurus and bos indicus x bos taurus breed types in a dry, temperate climate: performance of mature dams.mature dams representing hereford, red poll, f1 hereford x red poll, f1 red poll x hereford, f1 angus x hereford, f1 angus x charolais, f1 brahman x hereford and f1 brahman x angus breed types were evaluated. all cows were bred to limousin sires to produce two-way or three-way-cross progeny. mature brahman x hereford dams produced a higher (p less than .05) percentage of live calves than herefords, but dam breed differences in percentage of calves weaned relative to the number of cows exposed fo ...19902332395
immunization of crossbred cattle (bos indicus x bos taurus) with fractionated midgut antigens against hyalomma anatolicum anatolicum.crossbred calves (bos indicus x bos taurus) were immunized with a fractionated midgut supernate antigen (gs-f ag from hyalomma anatolicum anatolicum). the first inoculation on day 0 was given intramuscularly after emulsification with freund's complete adjuvant; the second was given subcutaneously on day 14 in incomplete freund's adjuvant; and the third on day 35 was given subcutaneously without adjuvant. each injection comprised 1 mg of antigen protein. ten days after the last inoculation, the i ...200314690089
nulliparous versus primiparous crossbred females for beef.feedlot and carcass traits of nulliparous and primiparous females representing eight breed types, including bos taurus and bos indicus x bos taurus crosses, were evaluated. nulliparous females (heifers) were in the feedlot for 4 mo; primiparous females (heiferettes) were fed for 2 1/3 mo after their calves were weaned at 6 mo of age. heifers averaged higher (p less than .01) in dressing percentage, percentage of kidney fat, carcass grade (p less than .10), and color of lean (p less than .05) com ...19912071505
physiological responses of newborn bos indicus and bos indicus x bos taurus calves after exposure to cold.brahman (n = 9) and 1/2 simmental x 1/4 brahman x 1/4 hereford (n = 11) calves were utilized to determine the influence of exposure to cold on the physiology of the neonate. all calves were removed from their dams within 20 min of birth and prior to suckling. calves were assigned randomly within breed to either a warm (w; 31 degrees c) or cold (c; 4 degrees c) environmental treatment group. jugular blood samples were collected via indwelling catheters at 20-min intervals for 180 min. at 100 to 1 ...19912005021
effects of breed group by location interaction on crossbred cattle in nebraska and florida.data on 2,744 calves produced in clay center, nebraska and brooksville, florida were used to evaluate the importance of genotype x location interactions on the reproductive and maternal performance of eight breed groups of f1 crossbred cows. a total of 648 f1 crossbred cows included bos taurus x bos taurus (bt x bt) crosses: hereford x angus reciprocal crossbreds (ha and ah), pinzgauer x angus (pa), pinzgauer x hereford (ph); and bos indicus x bos taurus (bi x bt) crosses: brahman x angus (ba), ...19912005003
life-cycle biological efficiency of bos indicus x bos taurus and bos taurus crossbred cow-calf production to weaning.a deterministic model was developed that accounted for all biological inputs and outputs for a theoretical herd of f1 females at age equilibrium mated to produce three-way terminal-cross calves and for the required proportion of straightbred cows needed to produce replacements. two bos indicus x bos taurus vs two bos taurus x bos taurus types of crossbred cows were compared in the production environment of south-central nebraska. the four types of f1 females were from hereford (h) or angus (a) d ...19911938641
output/input differences among nonpregnant, lactating bos indicus-bos taurus and bos taurus-bos taurus f1 cross cows.nonpregnant f1 crossbred cows, progeny of either hereford (h) or angus (a) dams and sired by brahman (bm), sahiwal (sw), pinzgauer (pz), h, or a sires, were fed to maintain initial weight while rearing charolais (c)-sired progeny for a period of 126 d in drylot commencing at about 48 d postpartum. cow-calf pairs were assigned to equalize cow age, calf sex, and breed of cow's dam among three replicate pens of approximately 12 pairs each. cows and calves were weighed every 2 wk and feed intake was ...19911894550
metabolizable energy for maintenance of beef-type bos taurus and bos indicus x bos taurus cows in a dry, temperate climate.metabolizable energy for maintenance (mem) was estimated using 123 mature cows of eight diverse breed groups. cows in each breed group were allotted at random 1) to limit-feeding to approximate maintenance or 2) to ad libitum access to feed. the mem values were calculated by regression of change in body energy on me intake. the mem values for mature hereford, red poll, hereford x red poll, red poll x hereford, angus x hereford, angus x charolais, brahman x hereford, and brahman x angus breed typ ...19911885390
life span of beef-type bos taurus and bos indicus x bos taurus females in a dry, temperate climate.females representing hereford, red poll, f1 hereford x red poll, f1 red poll x hereford, f1 angus x hereford, f1 angus x charolais, f1 brahman x hereford, and f1 brahman x angus breed types were evaluated from birth until 10 yr of age. of 308 females born alive, 35.7% died or were culled because of injury, serious illness, or reproductive failure. breed types differed (p less than .01) in total number of mating seasons per cow and total number of progeny born and weaned. values for lifetime tota ...19911885355
[carbohydrate content and glycolysis enzymatic activity in the liver of young purebred and hybrid cattle of both sexes].it is established that the content of glycogen, glucose, fructose and the activity of phosphoglucomutase and phosphogexoisomerase is considerably higher in the heifer liver and the content of pentoses of adenylic system and the activity of aldolase is essentially lower than in the liver of bulls of the same breed. in the liver of growing hybrid young cattle (except of the hereford heifers) as compared to the purebred ones the value of the total pentoses: glycogen and pentoses of adenylic system: ...1976941306
[studies of beef performance traits in piemontese x nanyang crossbred using microsatellites as genetic markers].five microsatellites, idvga-2, idvga-27, idvga-46, idvga-55 and tgla-44 were associated to beef performance traits of 100 heads of nanyang cattle, piemontese and their hybrid cattle using general linear model (glm) with statistical analysis system (sas). for microsatellite idvga-27, allele 136 had a positive relation with heart girth, height at hip cross and chest depth (body measurements) and loin width (muscularity evaluation); however, allele 142 showed a significant negative correlation with ...199910876662
cross-bred cattle protected against hyalomma anatolicum anatolicum by larval antigens purified by immunoaffinity chromatography.antigens from larvae of hyalomma anatolicum anatolicum were extracted and purified by immunoaffinity chromatography using immunoglobulin ligands from cross-bred animals immunized with soluble larval antigen. affinity-purified antigen (aff-tle) and a total larval extract (tle) were used to immunize cross-bred (bos indicus x bos taurus) cattle. the group immunized with aff-tle rejected 71.6% of larvae and 77.3% of nymphs. however, the rejection percentages were lower in the tle-immunized group. no ...199910509419
heat tolerance of boran and tuli crossbred steers.experiments were conducted to evaluate the heat tolerance of the following breeds: hereford (h), brahman (b), h x b, h x boran (h x bo), and h x tuli (h x t). heat tolerance was evaluated in a climatically controlled room (exp. 1) and under summer environmental conditions (exp. 2) by comparing rectal temperatures (rt), respiration rates (rr), and sweating rates (sw). in exp. 1, under extremely hot conditions (mean temperature-humidity index [thi] > 90), purebred b had significantly (p < .05) low ...199910492446
a primary screen of the bovine genome for quantitative trait loci affecting carcass and growth traits.a primary genomic screen for quantitative trait loci (qtl) affecting carcass and growth traits was performed by genotyping 238 microsatellite markers on 185 out of 300 total progeny from a bos indicus x bos taurus sire mated to bos taurus cows. the following traits were analyzed for qtl effects: birth weight (bwt), weaning weight (ww), yearling weight (yw), hot carcass weight (hcw), dressing percentage (dp), fat thickness (ft), marbling score (mar), longissimus muscle area (lma), rib bone (ribb) ...199910375215
effects of time of suckling during the solar day on duration of the postpartum anovulatory interval in brahman x hereford (f1) cows.previously published reports have indicated that postpartum anovulatory intervals can be markedly reduced and rebreeding performance enhanced in bos taurus cows by eliminating nighttime suckling. we sought to confirm this hypothesis by examining the effects of day, nighttime, and ad libitum suckling on suckling behavior of calves, duration of the postpartum anovulatory interval, and pregnancy rates in 45 fall-calving brahman x hereford (f1) cows. beginning on d 9 to 12 postpartum, calves were re ...199910340568
[selection in "hybrid" cattle populations].natural selection in populations of cattle was studied. heterozygosity level, pure-blooded proportion, stabilizing selection, and directional selection ii acted as quantitative traits. changes in the proportion of pure bloods with time were found, something not taken into account in breeding practice.19968964481
the polled locus maps to bta1 in a bos indicus x bos taurus cross.two hundred and nine reciprocal backcross and f2 progeny produced by embryo transfer from angus (bos taurus) and brahman (bos indicus) parents and their 60 parents and grandparents were utilized to localize the locus (poll) responsible for the polled phenotype in a genetic map of bovine chromosome 1. progeny were scored for polled, scurred, and horned phenotypes at 1 year of age and again following skull disection at slaughter at 20 months of age. phenotype frequencies were independent of gender ...19968830095
relationship between shear force and trained sensory panel tenderness ratings of 10 major muscles from bos indicus and bos taurus cattle.the present experiments were conducted to determine 1) the relationship between shear force and overall tenderness of 10 major beef muscles, 2) the effect of bos indicus inheritance on the tenderness of various beef muscles, 3) whether differences in tenderness between genotype are affected by method of cookery, and 4) the relationship between tenderness of the longissimus and tenderness of other muscles. to meet the first objective, shear force and trained sensory panel overall tenderness were ...19958586592
logistic regression analysis of pregnancy rate following transfer of bos indicus embryos into bos indicus x bos taurus heifers.factors affecting pregnancy rate of 5627 zebu embryos in crossbred females with unknown proportions of holstein and zebu breeding were examined. after evaluation for developmental stage, quality, and viability, embryos were immediately transferred to recipients. pregnancy diagnosis was conducted approximately 53 d after transfer; pregnancy rate was coded as a binomial event and analyzed using logistic regression models. maximum likelihood methodology and the likelihood ratio statistic were used ...200716979229
effect of marbling degree on beef palatability in bos taurus and bos indicus cattle.this study was designed to evaluate the relationship between marbling score and breed-type (bos taurus vs < or = 1/4 bos indicus) on palatability of cooked beef. one thousand six hundred sixty-seven steers and heifers (1,337 bos taurus and 330 bos indicus x bos taurus crosses) that had been managed and fed alike were used. shear force and tenderness rating indicated that meat from bos indicus cattle was less tender (p < .05) than meat from bos taurus cattle, regardless of marbling score. meat fr ...19947759364
non-specific immunization against bovine tropical theileriosis (theileria annulata) using killed corynebacterium parvum.killed corynebacterium parvum was used as an adjuvant for the production of non-specific resistance against theileria annulata in cattle. groups of cross-bred (bos indicus x bos taurus) calves were administered c. parvum adjuvant subcutaneously and were then challenged with t. annulata-infected ticks on 45, 60 or 90 days later. the challenge caused mild reactions in the protected calves. none of the 10 immunized calves died due to theileriosis, whereas all three paris of susceptible control calv ...19836685397
immunoprotective efficacy of a purified 39 kda nymphal antigen of hyalomma anatolicum anatolicum.soluble nymphal antigens (hnag) were purified by immunoaffinity chromatography using cnbr-activated sepharose 4b coupled with immunoglobulin ligands from animals immunized with hnag and 69-71% protected against challenge infestations, and 8% recovery of the purified protein (aff-hnag) was obtained. following immunization of crossbred calves (bos indicus x bos taurus) with 1600 sg of aff-hnag in three divided doses, significant rejections of larvae (p<0.001, 84.2%), nymphs (p<0.05, 61.4%) and adu ...200111254071
purebred-crossbred performance and genetic evaluation of postweaning growth and carcass traits in bos indicus x bos taurus crosses in australia.growth and carcass data on 7,154 cattle from a purebred project and 1,241 cattle from a crossbred project, comprising 916 first-crosses and 325 purebred brahman controls, were analyzed to estimate genetic parameters, including the genetic correlations between purebred and crossbred performance (rpc). the data also allowed the estimation of sire breed means for various growth and carcass traits. crossbred calves were produced using 9 angus, 8 hereford, 7 shorthorn, 14 belmont red, and 8 santa ger ...200212162647
growth traits and composition of two- and three-way-cross intact male progeny of bos taurus and bos indicus x bos taurus dams.feedlot traits, carcass traits and distribution of commercial cuts of crossbred intact male progeny (n = 556) from young and mature hereford, red poll, hereford x red poll, red poll x hereford, angus x hereford, angus x charolais, brahman x hereford and brahman x angus dams were evaluated. first-calf heifers were bred to red angus bulls; santa gertrudis sires were used for each cow's second and third breeding seasons. calves from these young dams were slaughtered at 13 mo. calves of mature dams ...19873610866
synchronization of bos indicus x bos taurus cows for timed artificial insemination using gonadotropin-releasing hormone plus prostaglandin f2alpha in combination with melengestrol acetate.nonlactating bos indicus x bos taurus cows were used in three herds to determine the efficacy of different pgf2alpha treatments in combination with gnrh and melengestrol acetate (mga) for a timed artificial insemination protocol. the start of the experiment was designated as d 0, at which time cows were assigned a body condition score and received 100 microg of gnrh. cows were fed mga (0.5 x mg x cow(-1) x d(-1)) on d 1 to 7. on d 7, cows received either a single injection of pgf2alpha (lutalyse ...200312723069
effects of scrotal insulation on sperm production, semen quality, and testicular echotexture in bos indicus and bos indicus x bos taurus bulls.the objectives of the present study were to evaluate the effects of scrotal insulation on sperm production, semen quality, and testicular echotexture in bos indicus and bos indicus x bos taurus crossbred bulls. in one experiment, b. indicus bulls (n=12) were allocated to control and whole-scrotum insulation groups, while in a second experiment, crossbred bulls (n=21) were allocated into control, whole-scrotum, and scrotal-neck insulation groups. insulation was applied for 4 days (start of insula ...200312853175
detection of quantitative trait loci for growth and carcass composition in cattle.the objective of the present study was to detect quantitative trait loci for economically important traits in a family from a bos indicus x bos taurus sire. a brahman x hereford sire was used to develop a half-sib family (n = 547). the sire was mated to bos taurus cows. traits analyzed were birth (kg) and weaning weights (kg); hot carcass weight (kg); marbling score; longissimus area (cm2); usda yield grade; estimated kidney, pelvic, and heart fat (%); fat thickness (cm); fat yield (%); and reta ...200314677852
different measures of energetic efficiency and their phenotypic relationships with growth, feed intake, and ultrasound and carcass merit in hybrid cattle.residual feed intake (rfi) has been proposed as an index for determining beef cattle energetic efficiency. although the relationship of rfi with feed conversion ratio (fcr) is well established, little is known about how rfi compares to other measures of efficiency. this study examined the phenotypic relationships among different measures of energetic efficiency with growth, feed intake, and ultrasound and carcass merit of hybrid cattle (n = 150). dry matter intake, me intake (mei), adg, metaboli ...200415318746
reproductive response in supplemented heifers in the humid tropics of costa rica.to evaluate the effect of two supplementary diets to determine the consequence on productive and reproductive performance in heifers (bos indicus x bos taurus) averaging between 24 and 36 months of age and grazing tropical pastures, two trials were conducted. thirty animals (initial bw 325.1+/-33.6 kg) were divided in two groups in the initial study: supplemented (sg) and control (cg); sg received a concentrate (5.5% cp and 2.85 mcal/kg of de dry matter basis) at 1% of body weight (bw). in the s ...200616137846
improving in vitro development of cloned bovine embryos with hybrid (holstein-chinese yellow) recipient oocytes recovered by ovum pick up.in the present study, oocytes from f1 hybrid cattle, as well as their parental lines, were recovered by ovum pick up (opu) and used as recipient cytoplasm for somatic cell nuclear transfer (scnt). four f1 hybrid (holstein dam x chinese yellow sire), 10 holstein and four chinese yellow cattle were subjected to opu once weekly. there were no significant differences among breeds for number of recovered oocytes per session (overall average, 7.8+/-0.5; mean+/-s.e.m.), quality of the recovered oocytes ...200516139603
lack of association of brucellosis resistance with (gt)(13) microsatellite allele at 3'utr of nramp1 gene in indian zebu (bos indicus) and crossbred (bos indicus x bos taurus) cattle.natural resistance associated macrophage protein 1 (nramp1), an integral transmembrane protein, is reported to influence the intraphagosomal microbial replication and thereby confer resistance to several intracellular pathogens in mice. in bovine, a significant association of (gt)(13) allelic variant of polymorphic microsatellite at 3' untranslated region (utr) of nramp1 gene with natural resistance to brucellosis has been established. the present study was aimed to detect polymorphism at 3'utr ...200516257497
assessing the relative ages of admixture in the bovine hybrid zones of africa and the near east using x chromosome haplotype mosaicism.historical hybridization events between the two subspecies of cattle, bos taurus and b. indicus, have occurred in several regions of the world, while other populations have remained nonadmixed. we typed closely linked x chromosome microsatellites in cattle populations with differing histories of admixture from africa, europe, the near east, and india. haplotype breakdown will occur as admixed populations age, and longer ancestral haplotypes will remain intact in more recently admixed populations ...200616582445
divergent development of testosterone secretion in male zebu (bos indicus) and crossbred cattle (bos indicus x bos taurus) and buffaloes (bubalus bubalis) during growth.delayed pubertal development and low fertility of bos indicus x bos taurus crossbred male cattle and domestic buffaloes is hardly understood hence, a sensitive enzymeimmunoassay (eia) was developed using the second antibody-coating technique and testosterone-3-o-carboxymethyloxime-horseradish peroxidase conjugate as a label for determination of testosterone in blood plasma. the eia was validated by standard criteria. blood samples were collected by venipuncture from growing male cattle (karan fr ...201020213223
fertility of bos indicus and bos indicus x bos taurus crossbreed cattle after estrus synchronization.ninety-five cows (79 boran and 16 boran-brahman crossbreeds) and 107 heifers (55 boran and 52 boran x friesian f1 crossbreeds) were used to determine estrus response, estrus response interval and pregnancy rate following synchronization with prostaglandin (pgf(2)alpha), a progesterone-releasing intravaginal device (prid) and synchro-mate b (smb). the proportion of cattle responding to synchronization treatment was 62.5, 43.5 and 57.7% for cows and 85.7, 68.0 and 81.5% for heifers using pgf(2)alp ...198916726556
response of prepubertal bos taurus and bos indicus x bos taurus heifers to melengestrol acetate with or without gonadotropin-releasing hormone.the effectiveness of treatments to induce estrus in prepubertal beef heifers was evaluated. angus x hereford (n = 148) and brahman x hereford (n = 148) heifers were sorted after weaning by body weight into light and heavy weight blocks. heifers were assigned to diets, calculated to reach a target weight of 55% or 65% of their projected mature weight by the start of breeding. cyclicity was determined after a 160-d observation period and from concentrations of progesterone in serum determined 10 d ...199016726762
evaluation of three estrus synchronization regimens for use in extensively managed bos indicus and bos indicus/taurus heifers in northern australia.two herds of extensively managed yearling bos indicus and bos indicus x bos taurus heifers (n=169) were assigned to one of three estrus synchronization treatment groups. the treatments consisted of 1) two injections of the prostaglandin (f2alpha) analogue, luprostiol (7.5 mg), given 12-d apart; 2) insertion of an intravaginal progesterone-releasing device (cidr-b), followed by 400 iu i.m. pregnant mare serum gonadotrophin (pmsg) and 7.5 mg luprostinol at cidr-b removal or 3) subcutaneous progest ...199116726986
effect of different levels and sources of zinc supplementation on quantitative and qualitative semen attributes and serum testosterone level in crossbred cattle (bos indicus x bos taurus) bulls.an experiment was conducted on 16 crossbred bulls (about 2 years of age, 316.2+/-0.77 kg average body weight), divided into groups i, ii, iii and iv to study the effect of different levels of zn supplementation from inorganic and organic sources on semen quality. the animals in the first 3 groups were supplemented with 0, 35 and 70 ppm zn from zn sulfate, respectively and the animals in-group iv were supplemented with 35 ppm zn as zn propionate. semen collection and evaluation was done in the fi ...200617169313
evaluation of reciprocal differences in bos indicus x bos taurus backcross calves produced through embryo transfer: i. birth and weaning traits.angus (a) and bos indicus (b; brahman or nellore) reciprocal backcross, embryo transfer calves, belonging to 28 full-sib families, were evaluated for differences in birth weight, gestation length, and weaning weight. two methods were investigated; method i made no distinction between how the f(1) parents were produced, whereas method ii distinguished between the 2 types of f(1) parents (ab vs. ba corresponding to a x b vs. b x a, respectively). bos indicus backcross calves had a 4.3 d longer (p ...200717235021
evaluation of reciprocal differences in bos indicus x bos taurus backcross calves produced through embryo transfer: ii. postweaning, carcass, and meat traits.angus (a) x bos indicus (b; brahman or nellore) reciprocal backcross, embryo transfer calves belonging to 28 full-sib families were evaluated for differences in feedyard initial bw, feedyard final bw, carcass weight, lm area, adjusted fat thickness, intramuscular fat, and warner-bratzler shear force. two methods of analysis were investigated; method i made no distinction between how the f(1) parents were produced, whereas method ii distinguished the 2 types of f(1) parents (ab vs. ba, correspond ...200717235022
vaccine potential of recombinant antigens of theileria annulata and hyalomma anatolicum anatolicum against vector and parasite.in an attempt to develop vaccine against hyalomma anatolicum anatolicum and theileria annulata, three antigens were expressed in prokaryotic expression system and protective potentiality of the antigens was evaluated in cross bred calves. two groups (grs. 1 and 4) of male cross-bred (bos indicus × bos taurus) calves were immunized with rhaa86, a bm86 ortholog of h. a. anatolicum, while one group of calves (gr. 2) were immunized with cocktails of two antigens viz., surface antigens of t. annulata ...201222546546
tasco supplementation: effects on carcass characteristics, sensory attributes, and retail display shelf-life.two hundred crossbred cattle (bos indicus x bos taurus) were supplemented with 2% tasco (ascophyllum nodosum) in a commercial finishing facility to evaluate marbling score, usda quality grade, sensory traits, and retail display shelf life. treatment animals (n = 100) received a steam-rolled corn (zea mays)-based diet containing 2% tasco meal (dm basis), for 14 d beginning at d 45 of the finishing period and again 14 d before slaughter. control animals (n = 100) received a steam-rolled corn (zea ...200717296779
sexual development in early- and late-maturing bos indicus and bos indicus x bos taurus crossbred bulls in brazil.two experiments were conducted to evaluate sexual development in early- and late-maturing nelore (bos indicus) and canchim (3/8 bos indicus x 5/8 bos taurus crossbred) bulls and to determine predictors of sexual precocity, and pubertal and maturity status. in experiment 1, 12 nelore bulls where examined from 300 to 900 days of age. puberty was characterized by an ejaculate containing > or =50 million sperm with > or =10% motile sperm, and maturity by an ejaculate containing > or =70% morphologic ...200415325547
ascophyllum nodosum supplementation: a preharvest intervention for reducing escherichia coli o157:h7 and salmonella spp. in feedlot steers.ascophyllum nodosum (tasco-14) decreased the prevalence of enterohemorrhagic escherichia coli (ehec) o157:h7 in animals fed prior to harvest. tasco-14 was supplemented on a 2% dry matter basis 14 days prior to harvest to determine its effects on ehec and salmonella spp. prevalence. two hundred mixed crossbred steers and heifers (bos indicus x bos taurus), in a large commercial finishing facility, served as experimental units. treatment (trt, n = 100) animals received a steam-rolled corn-based di ...200415453570
comparison of four synchronization protocols for fixed-time bovine embryo transfer in bos indicus x bos taurus recipients.the objective was to evaluate the effects of 400 iu of ecg given on days 5 or 8 of an estrus synchronization protocol with progesterone-releasing intravaginal devices (prid) and estradiol benzoate (eb), in recipients for fixed-time embryo transfer. a secondary objective was to determine the effects of injectable progesterone (given concurrent with eb treatment). three-hundred-and-four crossbred bos taurus x bos indicus beef heifers were randomly assigned to one of four treatment groups (2 x 2 fa ...200415511545
identification of bovine and novel interferon-tau alleles in the american plains bison (bison bison) by analysis of hybrid cattle x bison blastocysts.the objective of this study was to generate bison x cattle hybrid embryos by in vitro fertilization, to assess their developmental potential, to determine the pattern of secretion of the embryonic signaling molecule interferon-tau (ifn-tau), and to identify novel ifn-tau mrna polymorphism in the american plains bison. a total of 600 bovine oocytes were inseminated with frozen-thawed bison semen. of these, 40.7% cleaved and 14.8% proceeded to the blastocyst stage. individual blastocysts were cult ...200515570611
polymorphisms in the bovine leptin promoter associated with serum leptin concentration, growth, feed intake, feeding behavior, and measures of carcass merit.leptin is the hormone product of the obese gene synthesized and secreted predominantly by white adipocytes. it functions as a lipostatic signal regulating bw, food intake, energy expenditure, reproduction, and certain immune system functions. although previous studies have identified polymorphisms in the coding regions of the leptin gene in cattle that show considerable associations with feed intake, milk quality and quantity, and carcass fatness, no such associations have been reported for the ...200515583038
effect of heat stress on the expression profile of hsp90 among sahiwal (bos indicus) and frieswal (bos indicus × bos taurus) breed of cattle: a comparative study.we evaluated the effect of thermal challenge on the expression profile of heat shock protein 90 (hsp90) among sahiwal (bos indicus) and frieswal (bos indicus × bos taurus) breeds of cattle. the present investigation was focused on the comparative studies on hsp90 expression among frieswal and sahiwal under in vitro and environmental heat stress. measured immediately after the in vitro heat shock to the peripheral blood mononuclear cells (pbmcs), the relative expression of hsp90 mrna was signific ...201424333856
pre- and postweaning attributes in faunated and ciliate-free calves fed calf starter with or without fish meal.in a 2 x 2 factorial design, 24 newborn, crossbred (bos indicus x bos taurus) calves were distributed in 4 equal groups involving dietary treatments of prestarter diets with (fm) or without fish meal (nfm) in a faunated (f) or ciliate-free (d) ruminal environment to study the ruminal fermentative development in pre-and postweaning periods. defaunation was achieved by rearing calves in isolation and its effect was studied after first appearance of ciliate protozoa (observed after 8 wk of age) in ...200515905433
efficacy of either a single or split treatment of pgf2alpha after a 14 day melengestrol acetate treatment to synchronize estrus and induce luteolysis in bos indicus x bos taurus heifers.two experiments evaluated a modified delivery of prostaglandin f2alpha (pgf2alpha) after a melengestrol acetate (mga) treatment in angus and bos indicus x bos taurus (bi) heifers. experiment 1 was replicated three times with yearling bi heifers (n = 695). heifers received mga (0.5 mg head(-1) day(-1)) for 14 days. in replications 1 and 2, heifers received either 25 mg of pgf2alpha im 19 days after mga (single) or 12.5 mg of pgf2alpha im 19 and 20 days after mga (split). in replication 3, heifers ...200515955358
urinary excretion of purine derivatives in bos indicus x bos taurus crossbred cattle.four experiments were performed to study the kinetics of purine metabolism and urinary excretion in zebu crossbred cattle. fasting excretion was established in expt 1, using eighteen male bos indicus x bos taurus crossbred cattle (261 (se 9.1) kg body weight), six of each of the following genotypes: 5/8 bos indicus, 1/2 bos indicus and 3/8 bos indicus. no significant differences were observed among genotypes in fasting purine derivative excretion (277.3 (se 35.43) micromol/metabolic body weight) ...200516022751
influences of diet during gestation on potential postpartum reproductive performance and milk production of beef heifers.the influences of nutritional protein and energy during early and mid pregnancy on milk production and postpartum reproductive parameters were determined in 70 beef heifers of two composite breeds (bos indicus x bos taurus). at artificial insemination (ai), heifers were divided into four dietary treatment groups identified by the level of protein, and to a lesser extent energy, fed during the first and second trimesters: high/high (hh), high/low (hl), low/high (lh), and low/low (ll). milk produc ...200919796799
effect of protein supplementation on reproductive and productive performance in bos indicus x bos taurus heifers raised in the humid tropics of costa rica.in order to evaluate the effect of protein supplementation on the productive and reproductive performance of heifers, 45 bos indicus x bos taurus heifers, 673 +/- 146 days of age and weighing about 340 kg, were divided into two groups. the control group (n = 23) continued without supplementation, but the supplemented group (sg; n = 22) received concentrate at a rate of 1% bw kg per day. animals were adapted to the concentrate over a 15-day period and then supplemented for 30 days, after which es ...201019821163
hemato-biochemical changes, disease incidence and live weight gain in individual versus group reared calves fed on different levels of milk and skim milk.a 2 x 3 factorial design was used to study the impact of rearing systems, individual (i) versus group (g) and different levels of milk/skim milk feeding (f1, f2 and f3) on hemato-biochemical profile, disease incidence and average daily gain of crossbred (bos indicus x bos taurus) calves. six calves were taken in each group on the basis of their birth weight and housed in individual (2.20 x 1.16 m(2)/calf) or in group pens (2.20 x 1.03 m(2)/calf). after 3 days of colostrum feeding, calves were al ...200920163584
evaluation of two progestogen-based estrous synchronization protocols in yearling heifers of bos indicus × bos taurus breeding.yearling bos indicus × bos taurus heifers (n = 410) from three locations, were synchronized with either the select synch/cidr+timed-ai (ssc+tai) or 7-11+timed-ai (7-11+tai) treatments. on day 0 of the experiment, within each location, heifers were equally distributed to treatments by reproductive tract score (rts; scale 1-5: 1 = immature, 5 = estrous cycling) and body condition score. the 7-11+tai treatment consisted of melengestrol acetate (0.5 mg/head/d) from days 0 to 7, with pgf(2a) (25 mg i ...201121356555
prevalence of cattle herds with ivermectin resistant nematodes in the hot sub-humid tropics of mexico.the objective was to determine the prevalence of semi-intensive bos indicus and bos indicus+ùbos taurus cattle herds with ivermectin (ivm) resistant nematodes in a sub-humid tropical zone of mexico using the faecal egg count reduction test (fecrt). thirty-three herds (28 beef and 5 dual purpose herds) were monitored in a period of 6 months (september 2008 to february, 2009). only 14 of the 33 herds were included in the trial. the other herds had not enough animals with sufficient nematode eggs p ...201121831526
Tenderness profiles of ten muscles from F1 Bos indicus x Bos taurus and Bos taurus cattle cooked as steaks and roasts.Twenty Bos taurus (Hereford x Angus crosses) and 20 F1 Bos indicus x Bos taurus heifers of the same age, management and feeding regimen, were harvested and evaluated at 2days postmortem for carcass and meat traits. Ten muscles were obtained from the right sides and aged until 10days postmortem. Bos indicus carcasses were lighter, had less fat cover, smaller ribeyes, and less intramuscular lipid (all p=0.05). Bos taurus longissimus lumborum, gluteus medius, triceps brachii, and semimembranosus mu ...201122166845
admixture mapping of tuberculosis and pigmentation-related traits in an african-european hybrid cattle population.admixture mapping affords a powerful approach to genetic mapping of complex traits and may be particularly suited to investigation in cattle where many breeds and populations are hybrids of the two divergent ancestral genomes, derived from bos taurus and bos indicus. here we design a minimal genome wide snp panel for tracking ancestry in recent hybrids of holstein-friesian and local arsi zebu in a field sample from a region of high bovine tuberculosis (btb) endemicity in the central ethiopian hi ...201526124773
identification of a major locus interacting with mc1r and modifying black coat color in an f₂ nellore-angus population.in cattle, base color is assumed to depend on the enzymatic activity specified by the mc1r locus, i.e. the extension locus, with alleles coding for black (e(d)), red (e), and wild-type (e+). in most mammals, these alleles are presumed to follow the dominance model of e(d) > e+ > e, although exceptions are found. in bos indicus x bos taurus f2 cattle, some e(d)e+ heterozygotes are discordant with the dominance series for mc1r and display various degrees of red pigmentation on an otherwise predict ...201424460986
investigation of body and udder skin surface temperature differentials as an early indicator of mastitis in holstein friesian crossbred cows using digital infrared thermography technique.the objective of this study was to investigate the ability of infrared thermography (irt) technique and its interrelationship with conventional mastitis indicators for the early detection of mastitis in holstein friesian (hf) crossbred cows.201628096610
short-term dietary concentrate supplementation during estrus synchronization treatment in beef cows increased igf-i serum concentration but did not affect the reproductive response.the objective of this study was to evaluate if short-term dietary concentrate supplementation increased igf-i serum concentration and resulted in a reproductive response during estrus synchronization treatment in non-lactating beef cows. thirty non-lactating beef cows (bos indicus × bos taurus) were allocated to the same pastureland and fed native tropical grasses as a basal diet. cows were synchronized using a 7-day co-synch plus controlled internal drug release (cidr) protocol and received fix ...201727726060
select synch and co-synch protocols using a cidr yield similar pregnancy rates after a fixed-time insemination in suckled bos indicus × bos taurus cows.primiparous and multiparous suckled bos indicus × bos taurus cows were used to compare a select synch + controlled internal drug release (cidr) and timed artificial insemination (ai) protocol (ssc) to a modified co-synch + cidr protocol (cos). five separate groups of cows were utilized (n = 659) and within a group, cows were allotted to two treatments on the basis of body condition score (bcs) taken 10 days before and days postpartum at cidr insertion (day 0). blood samples were collected on day ...201626631280
the extent of linkage disequilibrium in a large cattle population of western africa and its consequences for association studies.several previous studies concluded that linkage disequilibrium (ld) in livestock populations from developed countries originated from the impact of strong selection. here, we assessed the extent of ld in a cattle population from western africa that was bred in an extensive farming system. the analyses were performed on 363 individuals in a bos indicus x bos taurus population using 42 microsatellite markers on bta04, bta07 and bta13. a high level of expected heterozygosity (0.71), a high mean num ...200717459015
technologies for fixed-time artificial insemination and their influence on reproductive performance of bos indicus cattle.the adaptation of bos indicus cattle to tropical and subtropical environments has led to their widespread distribution around the world. although artificial insemination (ai) is one of the best alternatives to introduce new genetics into bos indicus herds, the peculiarity of their temperament and the tendency to show short oestrus (many of them during the night) greatly affects the effectiveness of genetic improvement programs. therefore, the most useful alternative to increase the number of fem ...200717491150
energy and protein requirements for growth and maintenance of f1 nellore x red angus bulls, steers, and heifers.a comparative slaughter trial was conducted with 36 f1 nellore x red angus calves (12 steers, 12 bulls, and 12 heifers), averaging 274 kg of bw, to assess the net requirements of protein and energy for growth and maintenance. three cattle from each group (i.e., steers, bulls, and heifers) were slaughtered at the beginning of the trial to determine the initial body composition. the remaining calves were randomly assigned to 1 of 3 treatments: maintenance (diet containing 70% of dm as corn silage ...200717504963
effect of early luteolysis in progesterone-based timed ai protocols in bos indicus, bos indicus x bos taurus, and bos taurus heifers.the objective of this study was to evaluate the effects of treatment with an intravaginal progesterone-releasing device (cidr) and estradiol benzoate (eb) on follicular dynamics in bos indicus (n=23), bos taurus (n=25), and cross-bred (n=23) heifers. to assess the influence of reduced serum progesterone concentrations during 8 days of treatment with a progesterone-releasing device on follicular dynamics, half of the heifers received pgf at cidr insertion (day 0; 3 x 2 factorial design). mean (+/ ...200817980904
fixed-time artificial insemination with estradiol and progesterone for bos indicus cows ii: strategies and factors affecting fertility.in experiments 1, 2, and 3, we evaluated the effects of temporary weaning (tw), equine chorionic gonadotropin (ecg), and follicle-stimulating hormone (fsh) treatments on results of a fixed-time artificial insemination (tai) protocol in postpartum bos indicus cows. in experiment 1, treatment with 400 iu ecg or with tw for 48 h consistently improved pregnancy rates (prs) at tai, but, in experiment 2, fsh treatment was less effective than ecg or tw. in experiment 3, the inclusion of ecg treatment i ...200919344945
a novel polymorphism of gdf5 gene and its association with body measurement traits in bos taurus and bos indicus breeds.body measurement traits, influenced by genes and environmental factors, play numerous important roles in the value assessment of productivity and economy. growth differentiate factor 5 (gdf5), involved in the development and maintenance of bone and cartilage, is an important candidate gene for body measurement traits selection through marker-assisted selection (mas). in this study, based on the pcr-rflp technology, we discovered and evaluated the potential association of the single nucleotide po ...201019590978
pregnancy rates to fixed embryo transfer of vitrified ivp bos indicus, bos taurus or bos indicus × bos taurus embryos.the pregnancy rates obtained after the transfer of cryopreserved in vitro-produced (ivp) embryos are usually low and/or inconsistent. the objective of this study was to evaluate the pregnancy rates of holstein, gyr and holstein × gyr cattle after the transfer of vitrified ivp embryos produced with x-sorted sperm. seventy-two gyr and 703 holstein females were subjected to ovum pickup (opu) sessions, followed by in vitro embryo production using semen from sires of the same breeds. embryos (1636 ho ...201526280798
impact of a trace element supplementation programme on health and performance of cross-breed (bos indicus x bos taurus) dairy cattle under tropical farming conditions: a double-blinded randomized field trial.small-scale urban dairy farms (n = 16) in and around jimma, ethiopia with cross-bred (bos indicus × bos taurus) cows were enrolled in a double-blinded intervention study to investigate the effect of a trace element supplementation programme on trace element status and milk concentrations as well as performance [body condition score (bcs), milk yield, leptin], milk composition, antioxidant status (ferric-reducing ability of plasma (frap), thiobarbituric acid-reactive substances (tbars)], blood bi ...201524990384
a disparate trace element metabolism in zebu (bos indicus) and crossbred (bos indicus × bos taurus) cattle in response to a copper-deficient diet.copper deficiency is a commonly diagnosed problem in cattle around the globe. in jimma, ethiopia, 8 zebu (bos indicus) and 8 zebu ×: holstein friesian cross (bos taurus ×: bos indicus) heifers were used in an 11-wk study to investigate breed type differences and effects of cu deficiency on concentrations of trace elements in plasma and edible tissues as well as mrna expression of cu-related genes. heifers were fed a grass diet (6.4 ± 0.2 [sem] mg cu/kg dm) supplemented with 1 mg mo/kg dm in wk 1 ...201424829510
lipid droplet analysis using in vitro bovine oocytes and embryos.the aim of this study was to quantify the content of lipid droplets in bovine oocytes and embryos from bos indicus (bi), bos taurus (bt) and bos indicus × bos taurus (bi × bt). oocytes were aspirated post-mortem and subjected to in vitro maturation, in vitro fertilization and in vitro development; the medium employed at each stage (tcm-199, talp, sof) was supplemented with (i) serum replacement (sr), (ii) foetal calf serum (fcs) or (iii) oestrous cow serum (ecs). the structure and distribution o ...201424467659
molecular characterization, polymorphism and association study of lysozyme gene with milk production and somatic cell trait in bos indicus × bos taurus cattle.lysozyme, an anti-bacterial enzyme, is mostly found in the body fluids, various tissues and secretions of animals and humans, and confers immunity against a wide range of bacterial species. the present study was carried out to elucidate the gene sequence of this enzyme in indian sahiwal × holstein friesian crossbred cattle and to explore the polymorphism of the gene as well as their association with milk production and somatic cell traits. the total length of lysozyme cdna was found to be of 447 ...200922444439
factors affecting the first service conception rate of cows in smallholder dairy farms in bangladesh.the successful outcome of an insemination is a combination of both male and female fertility-linked factors. we investigated the first service conception rate of cows at artificial insemination (ai) in the smallholder dairy farms in bangladesh. frozen straws were prepared from ejaculates of bos indicus (n = 7) and bos indicus × bos taurus (n = 7) ai bulls. fertility was determined from 6101 first services in cows that were performed by 18 technicians in four regions between april 2004 and march ...201323106681
efficacy of rbm86 against rhipicephalus (boophilus) microplus (ivri-i line) and hyalomma anatolicum anatolicum (ivri-ii line) infestations on bovine calves.with an aim to evaluate the protective potentiality of rbm86 against rhipicephalus (boophilus) microplus indian veterinary research institute (ivri)-i line and hyalomma anatolicum anatolicum ivri-ii line infestations on crossbred (bos indicus × bos taurus) calves, 20 animals of 3 months of age were randomly divided in to four equal groups and maintained in tick-proof conditions. animals of groups 1 and 2 were immunized with 2 ml of rbm86 (100 μg)-based vaccine (procured from revetmex s.a. de c.v ...201222422293
identification of a null allele in genetic tests for bovine leukocyte adhesion deficiency in pakistani bos indicus × bos taurus cattle.two clinically healthy mature pakistani bos indicus × bos taurus cattle were genotyped as homozygous affected for the lethal immunodeficiency disorder bovine leukocyte adhesion deficiency (blad) using previously described pcr-rflp based dna tests which was confirmed by sequencing. sequencing of bos taurus and b. indicus × b. taurus genomic dna surrounding the disease causing mutation (c.383a > g) in the itgb2 gene identified numerous variations in exonic and intronic regions within and between s ...201222374219
use of a culture medium (mccoy®), as a method for evaluating bos indicus × bos taurus embryos.the aim of this study was to use culture medium (mccoy®) as a test to evaluate the classification of embryos after a primary grading using stereoscopic microscopy to further confirm whether embryos have been correctly scored by stereoscopic microscopy evaluating the level of apoptosis. forty-six bos indicus embryos were collected with a non-surgical method and evaluated with stereoscopic microscopy for scoring in three categories (good, fair and poor). cell proliferation and apoptosis were asses ...201222260644
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