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plant-feeding insects harbor double-stranded rna viruses encoding a novel proline-alanine rich protein and a polymerase distantly related to that of fungal viruses.novel double-stranded rnas (approximately 8 kbp) were isolated from threecornered alfalfa hopper (spissistilus festinus) and beet leafhopper (circulifer tenellus), two plant-feeding hemipteran insect pests. the two new viruses, designated spissistilus festinus virus 1 (spfv1) and circulifer tenellus virus 1 (citv1), do not appear to be encapsidated in conventional virions and shared a genome organization similar to that of several unclassified fungal viruses. spfv1 and citvl encode a proline-ala ...201020541786
genetic characterization of novel putative rhabdovirus and dsrna virus from japanese persimmon.deep-sequencing analysis of nucleic acids from leaf tissue of japanese persimmon trees exhibiting fruit apex disorder in some fruits detected two molecules that were graft transmitted to healthy seedlings. one of the complete genomes consisted of 13 467 nt and encoded six genes similar to those of plant rhabdoviruses. the virus formed a distinct cluster in the genus cytorhabdovirus with lettuce necrotic yellows virus, lettuce yellow mottle virus and strawberry crinkle virus in a phylogenetic tre ...201323704275
effects of aldicarb on nematodes, early season insect pests, and yield of soybean.the effects of aldicarb on soybean cyst (heterodera glycines) and root-knot (meloidogyne incognita and m. arenaria) nematode populations, early season insect pests and soybean (glycine max) yield were evaluated in five field experiments in northern and southern alabama. aldicarb significantly (p = 0.05) reduced nematode populations in only two cases: m. arenaria in centennial soybean in the wiregrass site and m. incognita in bedford soybean in a tennessee valley site. no significant difference ( ...198719290282
interaction among a nematode (heterodera glycines), an insect, and three weeds in soybean.a 2 x 3 x 4 factorial field experiment was established to determine the interaction among a nematode, an insect, and three weed species on soybean in 1983-86. low (nematicide treated) or high (untreated) population densities of the soybean cyst nematode (scn), heterodera glycines, and 0, 30, or 70% main stem girdling by the threecornered alfalfa hopper (tcah), spissistilus festinus, were combined with no weeds, one common cocklebur (cc), xanthium strumarium, one sicklepod (sp), cassia obtusifoli ...199019287788
influence of herbicide tolerant soybean production systems on insect pest populations and pest-induced crop damage.conventional soybean weed management and transgenic herbicide-tolerant management were examined to assess their effects on soybean insect pest populations in south georgia in 1997 and 1998. soybean variety had very little impact on the insect species observed, except that maturity group effects were observed for stink bug, primarily nezara viridula (l.), population densities on some sampling dates. stink bugs were more abundant on the early maturing varieties in mid-season. velvetbean caterpilla ...200312852606
insights into the ecology of grapevine red blotch virus in a diseased vineyard.limited information is available on the spread of grapevine red blotch virus (grbv, genus grablovirus, family geminiviridae) in vineyards. to investigate ecological aspects of red blotch disease spread, sticky cards to catch flying insects were placed in 2015 (april to november) and 2016 (march to november) in a vineyard study site in california where disease incidence increased by nearly 20% between 2014 and 2016. subsets of insect species or taxa were removed from sticky card traps and individ ...201828945519
distribution of glassy-winged sharpshooter and threecornered alfalfa hopper on plant hosts in the san joaquin valley, california.homalodisca vitripennis (germar) and spissistilus festinus (say) populations were surveyed bimonthly for 14 mo in kern county, ca, at five agricultural sites made up of a variety of potential host plants. in addition, s. festinus populations were surveyed in four alfalfa, medicago sativa l., fields in kern and tulare counties. insects were collected by beats-sweeps and sticky traps. data on host plant condition and phenology, and ground cover presence and composition were collected at the five a ...201020857711
phylogeny of geminivirus coat protein sequences and digital pcr aid in identifying spissistilus festinus as a vector of grapevine red blotch-associated virus.grapevine red blotch-associated virus (grbav) is a single-stranded dna virus, proposed to be a member of the family geminiviridae, and is associated with grapevines showing red blotch symptoms in north america. the existence of the virus was reported in 2012, and subsequently detected in grapevines in major grape production regions. we investigated if a vector exists that can transmit grbav in vineyards. phylogenetic analysis of the predicted amino acid sequence of coat protein (cp) of grbav wit ...201627111804
threecornered alfalfa hopper (hemiptera: membracidae): seasonal occurrence, girdle distribution, and response to insecticide treatment on peanut in south carolina.a survey of threecornered alfalfa hopper, spissistilus festinus (say) (hemiptera: membracidae), damage in 60 south carolina peanut, arachis hypogaea l., fields showed that 89 and 58% of plants had feeding girdles during 2003 and 2004, respectively. use of a foliar insecticide for other target pests reduced hopper damage. hopper damage was not affected by sampling distance from the field edge; therefore, injury was adequately assessed at 10 m from field borders. in-furrow insecticide choice, plan ...200717849875
capulavirus and grablovirus: two new genera in the family geminiviridae.geminiviruses are plant-infecting single-stranded dna viruses that occur in most parts of the world. currently, there are seven genera within the family geminiviridae (becurtovirus, begomovirus, curtovirus, eragrovirus, mastrevirus, topocuvirus and turncurtovirus). the rate of discovery of new geminiviruses has increased significantly over the last decade as a result of new molecular tools and approaches (rolling-circle amplification and deep sequencing) that allow for high-throughput workflows. ...201728213872
reovirus genomes from plant-feeding insects represent a newly discovered lineage within the family reoviridae.a complex set of double-stranded rnas (dsrnas) was isolated from threecornered alfalfa hopper (spissistilus festinus), a plant-feeding hemipteran pest. a subset of these dsrnas constitute the genome of a new reovirus, provisionally designated spissistilus festinus reovirus (spfrv). spfrv was present in threecornered alfalfa hopper populations in the san joaquin valley of california, with incidence ranging from 10% to 60% in 24 of 25 sample sets analyzed. the 10 dsrna segments of spfrv were compl ...201122142476
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