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malaria vaccine study site in irian jaya, indonesia: plasmodium falciparum incidence measurements and epidemiologic considerations in sample size estimation.malaria epidemiologic and entomologic studies were performed during both the high transmission and low transmission seasons to characterize the plasmodium falciparum malaria transmission at a proposed malaria vaccine trial site in irian jaya, indonesia. the study population consisted of two subsets: native irianese men with lifelong exposure to malaria and transmigrants who arrived from a nonmalarious area 2.5 years before the start of the study. all subjects received a radical cure for malaria ...19948116815
insecticide susceptibility status of anopheles koliensis (diptera: culicidae) in northeastern irian jaya, indonesia.anopheles koliensis, an important malaria vector in the interior region of irian jaya, indonesia, was evaluated for susceptibility to three different insecticide compounds using the standard world health organization diagnostic test kit and pretreated impregnated papers. a series of tests were conducted in arso pir i, a transmigrant settlement 60 km south of jayapura, from january 1988 to may 1989. all compounds were tested at the recommended diagnostic dosage and exposure time. an. koliensis we ...19938266243
altitude and the risk of bites from mosquitoes infected with malaria and filariasis among the mianmin people of papua new guinea.the mianmin are a mobile population occupying a remote lower montane area at 100-1200 m altitude in the north-western interior of papua new guinea (png). major medical problems include malaria and bancroftian filariasis. an entomological survey conducted along an altitudinal transect from 170 to 1000m identified anopheles koliensis as the predominant malaria vector below 650 m, with a. punctulatus dominating at the higher elevations. proportions of mosquitoes with malaria circumsporozoite antige ...19979093615
spatial and temporal variation in abundance of anopheles (diptera:culicidae) in a malaria endemic area in papua new guinea.abundance of anophelines in 10 villages in the wosera area of papua new guinea was monitored during 1990-1993. of 85,197 anophelines collected in 1,276 paired indoor and outdoor landing catches, 40.4% were anopheles koliensis owen, 36.7% an. punctulatus donitz, 14.3% an. karwari (james), 4.9% an. farauti s.l. laveran, 3.1%, an, longirostris brug, and 0.7% an. bancroftii giles. maps of average indoor biting rates were produced using a bayesian conditional autoregressive model which allowed for he ...19979103763
transmission intensity and its relationship to infection and disease due to wuchereria bancrofti in papua new guinea.this study describes the relationship between transmission intensity and infection and disease due to wuchereria bancrofti in an endemic area of papua new guinea. the prevalence of microfilaremia in the entire study population was 66%. of 1892 persons examined, 6.2% and 12.3% had lymphedema of the legs and hydroceles, respectively. the prevalences of microfilaremia and clinical morbidity were lowest in persons <20 years old and increased progressively with age. annual transmission potential and ...19979207373
comparison between anopheline mosquitoes (diptera: culicidae) caught using different methods in a malaria endemic area of papua new guinea.the mosquito sampling efficiency of cdc (centers for disease control) miniature light traps hung adjacent to mosquito nets, was compared with that of both indoor and outdoor human-bait collections in ten villages in the wosera area of papua new guinea. the most frequently collected anopheline in the matched indoor and light trap samples was anopheles koliensis owen, followed by a. punctulatus dönitz, a. karwari (james), a. farauti laveran (sensu lato), a. longirostris brug and a. bancroftii gile ...200010996862
subset partitioning of the ribosomal dna small subunit and its effects on the phylogeny of the anopheles punctulatus group.a phylogenetic study, based on maximum parsimony, of ten species in the anopheles punctulatus group of malaria vectors from the south-west pacific was performed using structural and similarity-based dna sequence alignments of the nuclear small ribosomal subunit (ssu = 18s). the structural alignment proved to be more informative than a computer generated similarity-based alignment. analyses involving the full structural sequence alignment (2169 bp) and the helical regions (1547 bp) resolved a sin ...200011029670
biting sites of anopheles koliensis on human collectors in papua new guinea.a field study was conducted to determine the preferred biting site of anopheles koliensis on the lower limbs of humans. this species showed a preference for biting the feet and ankles, rather than the legs, and when denied access to the feet and ankles, this species showed a preference for legs without hair, rather than legs with hair.200011081659
field evaluation of repellents containing deet and ai3-37220 against anopheles koliensis in papua new guinea.the repellent 1-(3-cyclohexen-1-yl-carbonyl)-2-methylpiperidine (ai3-37220) was compared with 2 formulations of diethylmethylbenzamide (deet) for its effectiveness in protecting 4 humans against the bites of anopheles koliensis mosquitoes at a village in central province, papua new guinea. a mean of 77.2 +/- 10.5 bites/human/10 min of an. koliensis was collected on ethanol-treated (control) volunteers, a much higher density than most previous studies with anopheles sp. mosquitoes. the protection ...200111345417
the effectiveness of light, 1-octen-3-ol, and carbon dioxide as attractants for anopheline mosquitoes in madang province, papua new guinea.the effectiveness of light, 1-octen-3-ol (octenol), carbon dioxide (co2) and a combination of co2 and octenol were compared as mosquito attractants using encephalitis vector surveillance traps in 2 villages in madang province, papua new guinea (png). five species were collected, anopheles koliensis, anopheles farauti 2, anopheles farauti 4, anopheles longirostris, and anopheles bancroftii. light alone was not attractive to any of these species, and the attractiveness of octenol alone, though gre ...200415532920
are insecticide-treated bednets more protective against plasmodium falciparum than plasmodium vivax-infected mosquitoes?the outcomes of insecticide-treated bednet (itn) interventions for malaria control in papua new guinea tend to suggest a differential protective effect against plasmodium falciparum and plasmodium vivax. little is known about the impact of itns on the relative abundance of mosquitoes infected with either p. falciparum or p. vivax. this paper describes the biting cycle of p. falciparum and p. vivax-infected mosquitoes and the impact of an itn intervention on the proportion of mosquitoes infected ...200616504027
therapeutic response of multidrug-resistant plasmodium falciparum and p. vivax to chloroquine and sulfadoxine-pyrimethamine in southern papua, indonesia.to determine the level of antimalarial drug resistance in southern papua, indonesia, we assessed the therapeutic efficacy of chloroquine plus sulfadoxine-pyrimethamine (cq+sp) for plasmodium falciparum infections as well as cq monotherapy for p. vivax infections. patients with p. falciparum failing therapy were re-treated with unsupervised quinine+/-doxycycline therapy and those with p. vivax with either unsupervised quinine+/-doxycycline or amodiaquine. in total, 143 patients were enrolled in t ...200717028048
two fixed-dose artemisinin combinations for drug-resistant falciparum and vivax malaria in papua, indonesia: an open-label randomised comparison.the burden of plasmodium vivax infections has been underappreciated, especially in southeast asia where chloroquine resistant strains have emerged. our aim was to compare the safety and efficacy of dihydroartemisinin-piperaquine with that of artemether-lumefantrine in patients with uncomplicated malaria caused by multidrug-resistant p falciparum and p vivax.200717336652
dihydroartemisinin-piperaquine versus artesunate-amodiaquine: superior efficacy and posttreatment prophylaxis against multidrug-resistant plasmodium falciparum and plasmodium vivax malaria.antimalarial drug resistance is now well established in both plasmodium falciparum and plasmodium vivax. in southern papua, indonesia, where both strains of plasmodia coexist, we have been conducting a series of studies to optimize treatment strategies.200717366451
adverse pregnancy outcomes in an area where multidrug-resistant plasmodium vivax and plasmodium falciparum infections are endemic.plasmodium falciparum infection exerts a considerable burden on pregnant women, but less is known about the adverse consequences of plasmodium vivax infection.200818419439
multidrug-resistant plasmodium vivax associated with severe and fatal malaria: a prospective study in papua, indonesia.multidrug-resistant plasmodium vivax (pv) is widespread in eastern indonesia, and emerging elsewhere in asia-pacific and south america, but is generally regarded as a benign disease. the aim of the study was to review the spectrum of disease associated with malaria due to pv and p. falciparum (pf) in patients presenting to a hospital in timika, southern papua, indonesia.200818563962
malaria morbidity in papua indonesia, an area with multidrug resistant plasmodium vivax and plasmodium falciparum.multidrug resistance has emerged to both plasmodium vivax and plasmodium falciparum and yet the comparative epidemiology of these infections is poorly defined.200818673572
in vivo and in vitro efficacy of amodiaquine monotherapy for treatment of infection by chloroquine-resistant plasmodium vivax.amodiaquine retains efficacy against infection by chloroquine-resistant plasmodium falciparum; however, little information is available on its efficacy against infection by chloroquine-resistant plasmodium vivax. patients presenting to a rural clinic with a pure p. vivax infection that recurred after recent antimalarial treatment were retreated, this time with amodiaquine monotherapy, and the risk of further recurrence within 4 weeks was assessed. of the 87 patients with pure p. vivax infection, ...200919104023
implementation of a novel pcr based method for detecting malaria parasites from naturally infected mosquitoes in papua new guinea.detection of plasmodium species in mosquitoes is important for designing vector control studies. however, most of the pcr-based detection methods show some potential limitations. the objective of this study was to introduce an effective pcr-based method for detecting plasmodium vivax and plasmodium falciparum from the field-caught mosquitoes of papua new guinea.200919646275
in vivo and in vitro efficacy of chloroquine against plasmodium malariae and p. ovale in papua, indonesia.reports of potential drug-resistant strains of plasmodium malariae in western indonesia raise concerns that chloroquine resistance may be emerging in p. malariae and p. ovale. in order to assess this, in vivo and in vitro efficacy studies were conducted in patients with monoinfection in papua, indonesia. consecutive patients with uncomplicated malaria due to p. ovale or p. malariae were enrolled in a prospective clinical trial, provided with supervised chloroquine treatment, and followed for 28 ...201020937779
multilocus haplotypes reveal variable levels of diversity and population structure of plasmodium falciparum in papua new guinea, a region of intense perennial transmission.the south west pacific nation of papua new guinea has intense year round transmission of plasmodium falciparum on the coast and in the low-lying inland areas. local heterogeneity in the epidemiology of malaria suggests that parasites from multiple locations will need to be surveyed to define the population biology of p. falciparum in the region. this study describes the population genetics of p. falciparum in thirteen villages spread over four distinct catchment areas of papua new guinea.201021092231
high throughput multiplex assay for species identification of papua new guinea malaria vectors: members of the anopheles punctulatus (diptera: culicidae) species group.malaria and filariasis are transmitted in the southwest pacific region by anopheles punctulatus sibling species including an. punctulatus, an. koliensis, the an. farauti complex 1-8 (includes an. hinesorum [an. farauti 2], an. torresiensis [an. farauti 3]). distinguishing these species from each other requires molecular diagnostic methods. we developed a multiplex polymerase chain reaction (pcr)-based assay specific for known species-specific nucleotide differences in the internal transcribed sp ...201121212222
bionomics of the malaria vector anopheles farauti in temotu province, solomon islands: issues for malaria elimination.abstract: background: in the solomon islands, the malaria eradication programmes of the 1970s virtually eliminated the malaria vectors: anopheles punctulatus and anopheles koliensis, both late night biting, endophagic species. however, the vector, anopheles farauti, changed its behaviour to bite early in the evening outdoors. thus, an. farauti mosquitoes were able to avoid insecticide exposure and still maintain transmission. thirty years on and the solomon islands are planning for intensified m ...201121592366
the dominant anopheles vectors of human malaria in the asia-pacific region: occurrence data, distribution maps and bionomic precis.abstract: background: the final article in a series of three publications examining the global distribution of 41 dominant vector species (dvs) of malaria is presented here. the first publication examined the dvs from the americas, with the second covering those species present in africa, europe and the middle east. here we discuss the 19 dvs of the asian-pacific region. this region experiences a high diversity of vector species, many occurring sympatrically, which, combined with the occurrence ...201121612587
Highly effective therapy for maternal malaria associated with a lower risk of vertical transmission.The epidemiology of congenital malaria was investigated in a hospital-based malaria surveillance study in Papua, Indonesia.201121908728
changes in vector species composition and current vector biology and behaviour will favour malaria elimination in santa isabel province, solomon islands.in 2009, santa isabel province in the solomon islands embarked on a malaria elimination programme. however, very little is known in the province about the anopheline fauna, which species are vectors, their bionomics and how they may respond to intensified intervention measures. the purpose of this study was to provide baseline data on the malaria vectors and to ascertain the possibility of successfully eliminating malaria using the existing conventional vector control measures, such as indoor re ...201121961990
mitochondrial genome sequences reveal deep divergences among anopheles punctulatus sibling species in papua new guinea.members of the anopheles punctulatus group (ap group) are the primary vectors of human malaria in papua new guinea. the ap group includes 13 sibling species, most of them morphologically indistinguishable. understanding why only certain species are able to transmit malaria requires a better comprehension of their evolutionary history. in particular, understanding relationships and divergence times among anopheles species may enable assessing how malaria-related traits (e.g. blood feeding behavio ...201323405960
multiplex assay for species identification and monitoring of insecticide resistance in anopheles punctulatus group populations of papua new guinea.anopheles punctulatus sibling species (an. punctulatus s.s., anopheles koliensis, and anopheles farauti species complex [eight cryptic species]) are principal vectors of malaria and filariasis in the southwest pacific. given significant effort to reduce malaria and filariasis transmission through insecticide-treated net distribution in the region, effective strategies to monitor evolution of insecticide resistance among an. punctulatus sibling species is essential. mutations in the voltage-gated ...022232465
key knowledge gaps for plasmodium vivax control and elimination.there is inadequate understanding of the biology, pathology, transmission, and control of plasmodium vivax, the geographically most widespread cause of human malaria. during the last decades, study of this species was neglected, in part due to the erroneous belief that it is intrinsically benign. in addition, many technical challenges in culturing the parasite also hampered understanding its fundamental biology and molecular and cellular responses to chemotherapeutics. research on vivax malaria ...201627430544
diversification of the genus anopheles and a neotropical clade from the late cretaceous.the anopheles genus is a member of the culicidae family and consists of approximately 460 recognized species. the genus is composed of 7 subgenera with diverse geographical distributions. despite its huge medical importance, a consensus has not been reached on the phylogenetic relationships among anopheles subgenera. we assembled a comprehensive dataset comprising the coi, coii and 5.8s rrna genes and used maximum likelihood and bayesian inference to estimate the phylogeny and divergence times o ...201526244561
mutations in the voltage-gated sodium channel gene of anophelines and their association with resistance to pyrethroids - a review.constant and extensive use of chemical insecticides has created a selection pressure and favored resistance development in many insect species worldwide. one of the most important pyrethroid resistance mechanisms is classified as target site insensitivity, due to conformational changes in the target site that impair a proper binding of the insecticide molecule. the voltage-gated sodium channel (nav) is the target of pyrethroids and ddt insecticides, used to control insects of medical, agricultur ...201425292318
entomological monitoring and evaluation: diverse transmission settings of icemr projects will require local and regional malaria elimination strategies.the unprecedented global efforts for malaria elimination in the past decade have resulted in altered vectorial systems, vector behaviors, and bionomics. these changes combined with increasingly evident heterogeneities in malaria transmission require innovative vector control strategies in addition to the established practices of long-lasting insecticidal nets and indoor residual spraying. integrated vector management will require focal and tailored vector control to achieve malaria elimination. ...201526259942
relationships between anopheline mosquitoes and topography in west timor and java, indonesia.malaria is a serious health issue in indonesia. mosquito control is one aspect of an integrated malaria management programme. to focus resources on priority areas, information is needed about the vectors and their habitats. this research aimed to identify the relationship between anopheline mosquitoes and topography in west timor and java.201020796265
comparative mt genomics of the tipuloidea (diptera: nematocera: tipulomorpha) and its implications for the phylogeny of the tipulomorpha.a traditionally controversial taxon, the tipulomorpha has been frequently discussed with respect to both its familial composition and relationships with other nematocera. the interpretation of internal relationships within the tipuloidea, which include the tipulidae sensu stricto, cylindrotomidae, pediciidae and limoniidae, is also problematic. we sequenced the first complete mitochondrial (mt) genome of symplecta hybrida (meigen, 1804), which belongs to the subfamily chioneinae of family limoni ...201627341029
characterizing, controlling and eliminating residual malaria transmission.long-lasting insecticidal nets (llins) and indoor residual spraying (irs) interventions can reduce malaria transmission by targeting mosquitoes when they feed upon sleeping humans and/or rest inside houses, livestock shelters or other man-made structures. however, many malaria vector species can maintain robust transmission, despite high coverage of llins/irs containing insecticides to which they are physiologically fully susceptible, because they exhibit one or more behaviours that define the b ...201425149656
entomological surveillance of behavioural resilience and resistance in residual malaria vector populations.the most potent malaria vectors rely heavily upon human blood so they are vulnerable to attack with insecticide-treated nets (itns) and indoor residual spraying (irs) within houses. mosquito taxa that can avoid feeding or resting indoors, or by obtaining blood from animals, mediate a growing proportion of the dwindling transmission that persists as itns and irs are scaled up.201323577656
treatment-seeking behaviour and associated costs for malaria in papua, indonesia.malaria remains a significant public health issue in eastern indonesia, where multidrug resistant plasmodium falciparum and plasmodium vivax are highly prevalent. the objective of this study was to describe treatment-seeking behaviour and household costs prior to a change to a unified treatment policy of dihydroartemisinin-piperaquine in mimika district, papua province in 2006.201627821127
asymptomatic vivax and falciparum parasitaemia with helminth co-infection: major risk factors for anaemia in early life.anaemia in children under five years old is associated with poor health, growth and developmental outcomes. in papua, indonesia, where the burden of anaemia in infants is high, we conducted a community survey to assess the association between plasmodium infection, helminth carriage and the risk of anaemia.201627504828
plasmodium vivax infection: a major determinant of severe anaemia in infancy.most malarious countries outside of africa are co-endemic for plasmodium falciparum and plasmodium vivax. the comparative burden of anaemia in the community caused by these two species is incompletely characterized.201627306221
frequent blood feeding enables insecticide-treated nets to reduce transmission by mosquitoes that bite predominately outdoors.the effectiveness of vector control on malaria transmission by long-lasting insecticidal nets (llins) and indoor residual spraying (irs) depends on the vectors entering houses to blood feed and rest when people are inside houses. in the solomon islands, significant reductions in malaria have been achieved in the past 20 years with insecticide-treated bed nets, irs, improved diagnosis and treatment with artemisinin combination therapies; despite the preference of the primary vector, anopheles far ...201626969430
whole-genome sequencing reveals absence of recent gene flow and separate demographic histories for anopheles punctulatus mosquitoes in papua new guinea.anopheles mosquitoes are the vectors of several human diseases including malaria. in many malaria endemic areas, several species of anopheles coexist, sometimes in the form of related sibling species that are morphologically indistinguishable. determining the size and organization of anopheles populations, and possible ongoing gene flow among them is important for malaria control and, in particular, for monitoring the spread of insecticide resistance alleles. however, these parameters have been ...201525677924
the bionomics of the malaria vector anopheles farauti in northern guadalcanal, solomon islands: issues for successful vector control.the north coast of guadalcanal has some of the most intense malaria transmission in the solomon islands. and, there is a push for intensified vector control in guadalcanal, to improve the livelihood of residents and to minimize the number of cases, which are regularly exported to the rest of the country. therefore, the bionomics of the target vector, anopheles farauti, was profiled in 2007-08; which was after 20 years of limited surveillance during which time treated bed nets (itns) were distrib ...201424528850
microsatellite and mitochondrial markers reveal strong gene flow barriers for anopheles farauti in the solomon archipelago: implications for malaria vector control.anopheles farauti is the primary malaria vector throughout the coastal regions of the southwest pacific. a shift in peak biting time from late to early in the night occurred following widespread indoor residue spraying of dichlorodiphenyltrichloro-ethane (ddt) and has persisted in some island populations despite the intervention ending decades ago. we used mitochondrial cytochrome oxidase i (coi) sequence data and 12 newly developed microsatellite markers to assess the population genetic structu ...201424440418
major burden of severe anemia from non-falciparum malaria species in southern papua: a hospital-based surveillance study.the burden of anemia attributable to non-falciparum malarias in regions with plasmodium co-endemicity is poorly documented. we compared the hematological profile of patients with and without malaria in southern papua, indonesia.201324358031
successful malaria elimination strategies require interventions that target changing vector behaviours.the ultimate long-term goal of malaria eradication was recently placed back onto the global health agenda. when planning for this goal, it is important to remember why the original global malaria eradication programme (gmep), conducted with ddt-based indoor residual spraying (irs), did not achieve its goals. one of the technical reasons for the failure to eliminate malaria was over reliance on a single intervention and subsequently the mosquito vectors developed behavioural resistance so that th ...201323388506
population structure, mitochondrial polyphyly and the repeated loss of human biting ability in anopheline mosquitoes from the southwest pacific.australia and new guinea contain high levels of endemism and biodiversity, yet there have been few evaluations of population-level genetic diversity in fauna occurring throughout the australo-papuan region. using extensive geographical sampling, we examined and compared the phylogenetic relationships, phylogeography and population structure of anopheles farauti, an. hinesorum and an. irenicus throughout their ranges in the southwest pacific using mitochondrial (mtdna coi) and nuclear (ribosomal ...201222747666
light traps fail to estimate reliable malaria mosquito biting rates on bioko island, equatorial guinea.the human biting rate (hbr), an important parameter for assessing malaria transmission and evaluating vector control interventions, is commonly estimated by human landing collections (hlc). although intense efforts have been made to find alternative non-exposure mosquito collection methods, hlc remains the standard for providing reliable and consistent hbrs. the aim of this study was to assess the relationship between human landing and light trap collections (ltc), in an attempt to estimate oper ...201222364588
phenotyping clinical resistance to chloroquine in plasmodium vivax in northeastern papua, indonesia.chloroquine (cq)-resistant plasmodium vivax was first documented in 1989 and threatens much of eastern indonesia, with > 50% of therapeutic failure rates. we screened 2236 subjects for malaria infection through active case detection and identified 232 infected cases with 100 subjects carried p. vivax mono infection. we prospectively evaluated therapeutic responses to cq in 73 subjects infected by p. vivax in northeastern papua, indonesia. we phenotyped these infections as susceptible or resistan ...201124533261
influence of environmental factors on the abundance of anopheles farauti larvae in large brackish water streams in northern guadalcanal, solomon islands.the main vector of malaria in solomon islands is anopheles farauti, which has a mainly coastal distribution. in northern guadalcanal, solomon islands, high densities of an. farauti are supported by large brackish streams, which in the dry season are dammed by localized sand migration. the factors controlling the high larval productivity of these breeding sites have not been identified. accordingly the influence of environmental factors on the presence and density of an. farauti larvae was assess ...201121910907
plasticity of host selection by malaria vectors of papua new guinea.host selection is an important determinant of vectorial capacity because malaria transmission increases when mosquitoes feed more on humans than non-humans. host selection also affects the outcome of long-lasting insecticidal nets (llin). despite the recent nationwide implementation of llin-based malaria control program in papua new guinea (png), little is known about the host selection of the local anopheles vectors. this study investigated the host selection of anopheles vectors in png.201728222769
a phylogenetic study of the anopheles punctulatus group of malaria vectors comparing rdna sequence alignments derived from the mitochondrial and nuclear small ribosomal subunits.a phylogenetic study of the members of the anopheles punctulatus group was performed using structural and similarity-based dna sequence alignments of the small ribosomal subunit (ssu) from both the nuclear and the mitochondrial genomes. the mitochondrial ssu gene (12s, approximately 650 bp) proved to be highly restricted by its secondary structure and displayed little informative sequence variation. consequently, it was considered unsuitable for a phylogenetic study of these closely related mosq ...200011133197
population genetics of anopheles koliensis through papua new guinea: new cryptic species and landscape topography effects on genetic connectivity.new guinea is a topographically and biogeographically complex region that supports unique endemic fauna. studies describing the population connectivity of species through this region are scarce. we present a population and landscape genetic study on the endemic malaria-transmitting mosquito, anopheles koliensis (owen). using mitochondrial and nuclear sequence data, as well as microsatellites, we show the evidence of geographically discrete population structure within papua new guinea (png). we a ...201931871651
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