| penaeid prawns and associated luminous bacteria. | luminous bacteria associated with the exoskeleton, gill and gut of penaeid prawns, penaeus indicus h. milne edwards and penaeus monodon fabricius in mangalore waters were isolated, identified and quantified. two species of bacteria, vibrio harveyi and vibrio fischeri were recorded, the former was dominant in the exoskeleton and gill of both the penaeids. gut of prawns supported exclusive and dense populations of v. harveyi suggesting that the species is well adapted to proliferate in this microe ... | 1994 | 7866726 |
| molecular mechanisms regulating molting in a crustacean. | crustacean growth and development is characterized by periodic shedding (ecdysis) and replacement of the rigid exoskeleton. secretions of the x-organ sinus gland complex control the cellular events that lead to growth and molting. western blot and elisa results showed a progressive increase in growth arrest-specific protein (gas7) from early postmolt stage to a maximum at late postmolt stage. phosphorylation of erk2, a downstream signaling protein, was also identified in the subsequent stages. e ... | 2006 | 16441669 |
| molecular screening, isolation, and characterization of enterohemorrhagic escherichia coli o157:h7 from retail shrimp. | foodborne outbreaks attributed to the contamination of foods with enterohemorrhagic escherichia coli (ehec) o157:h7 are a growing global concern. fish and shrimp samples obtained from different retail fish markets in cochin, india, were screened by direct pcr assays targeting three important virulence markers of ehec, the intimin protein (eaea gene), enterohemolysin (hlya gene), and shiga toxin (stx gene). one shrimp (fenneropenaeus indicus) sample was positive for all these virulence markers, a ... | 2010 | 20051211 |
| activities of membrane bound phosphatases, transaminases and mitochondrial enzymes in white spot syndrome virus infected tissues of fenneropenaeus indicus. | disease caused by viruses, especially white spot syndrome virus (wssv), present the greatest challenge to shrimp aquaculture worldwide. massive tissue disintegration occurs in wssv-infected ectodermal and mesodermal tissues of penaeid shrimp. the activities of membrane bound phosphatases (na(+)k(+)atpase, ca(2+)atpase, mg(2+)atpase and total atpase), transaminases (alanine transaminase (alt) and aspartate transaminase (ast)) and mitochondrial enzymes (isocitrate dehydrogenase (icdh), succinate d ... | 2006 | 16413626 |
| white spot syndrome virus infection decreases the activity of antioxidant enzymes in fenneropenaeus indicus. | white spot syndrome virus (wssv) is the causative agent of white spot disease of shrimp, causing mass mortalities in aquaculture. wssv infection causes oxidative stress by the release of reactive oxygen species (ros) that are toxic to the cells. the antioxidant enzymes associated with oxidative stress during the process of pathogenesis of wssv in the infected tissues (hemolymph, hepatopancreas, gills and muscle) of fenneropenaeus indicus were quantitatively determined at different time intervals ... | 2006 | 16139913 |
| detection of hepatopancreatic parvovirus (hpv) in wild shrimp from india by nested polymerase chain reaction (pcr). | the prevalence of hepatopancreatic parvovirus (hpv) in wild penaeid shrimp samples from india was studied by nested polymerase chain reaction (pcr) using primers designed in our laboratory. the virus could be detected in 9 out of 119 samples by non-nested pcr. however, by nested pcr 69 out of 119 samples were positive. the pcr results were confirmed by hybridization with digoxigenin-labelled dna probe. shrimp species positive by non-nested pcr included penaeus monodon, penaeus indicus and penaeu ... | 2005 | 15819441 |
| interaction of ph with mercuric chloride toxicity to penaeid prawns from a tropical estuary, east coast of india: enhanced toxicity at low ph. | toxicity tests were conducted to study the interaction of ph and the response of two size groups of penaeid prawns i.e. penaeus monodon and penaeus indicus to different sets of five concentrations of hgcl2 (0.01-0.09 mg l(-1)) under a broad range of ph conditions (5-9). behavioural responses varied according to test solution concentration and nominated values of ph. abnormality was detected in the higher concentrations with lower ph in smaller size group of p. monodon. high mortality was observe ... | 2005 | 15667843 |
| microsporidians in penaeid shrimp along the west coast of madagascar. | three species of penaeid shrimp, fenneropenaeus indicus, penaeus monodon and p. semisulcatus, found in trawler catches off the west coast of madagascar were infected with microsporidian parasites. the infections were evident as muscular lesions with a cottony appearance when abundant. spore size (2.6 x 1.6 microm) and morphology (ovoid) for the parasites infecting both f. indicus and p. semisulcatus were not significantly different, suggesting that they might be the same microsporidian species. ... | 2004 | 15038455 |
| response of penaeus indicus females at two different stages of ovarian development to a lethal infection with vibrio penaeicida. | an association between vitellogenesis and the immune system was suggested in crustaceans from studies on plasma lipoproteins. the present research studies the effect of an experimentally induced bacterial infection on vitellogenesis in females of the shrimp penaeus indicus, as a model for penaeid species. pre-vitellogenic and vitellogenic p. indicus females were experimentally infected with an extremely pathogenic bacterium, vibrio penaeicida. the peak in mortality occurred earlier in pre-vitell ... | 2003 | 12581716 |
| isolation and characterization of an acetyl group-recognizing agglutinin from the serum of the indian white shrimp fenneropenaeus indicus. | a natural agglutinin from the serum of the indian white shrimp fenneropenaeus (penaeus) indicus was purified to electrophoretic homogeneity by a single-step affinity chromatography on n-acetylglucosamine-sepharose 6b. the expression of hemagglutinating (ha) activity of f. indicus agglutinin (fia) was independent of the presence of divalent cations and insensitive to their chelators. fia gave a single symmetrical peak in its native form with a molecular mass estimate of 200 kda on gel filtration ... | 2002 | 12051684 |
| identification of tropomyosin as the major shrimp allergen and characterization of its ige-binding epitopes. | the major heat-stable shrimp allergen (designated as sa-ii), capable of provoking ige-mediated immediate type hypersensitivity reactions after the ingestion of cooked shrimp, has been shown to be a 34-kda heat-stable protein containing 300 amino acid residues. here, we report that a comparison of amino acid sequences of different peptides generated by proteolysis of sa-ii revealed an 86% homology with tropomyosin from drosophila melanogaster, suggesting that sa-ii could be the shrimp muscle prot ... | 1993 | 7693809 |
| isolation and characterization of heat-stable allergens from shrimp (penaeus indicus). | shrimp are among the more common causes of immediate hypersensitivity reactions to food. to characterize better the allergenic substances within shrimp, extracts from heated shrimp were systematically examined with solid-phase radioimmunoassay and sera from patients clinically sensitive to shrimp. two heat-stable protein allergens, designated as sa-i and sa-ii, were identified from boiled shrimp (penaeus indicus) extracts. sa-i was isolated by ultrafiltration, sephadex g-25, and diethylaminoethy ... | 1989 | 2913137 |
| functional characterization of a c-type lysozyme from indian shrimp fenneropenaeus indicus. | lysozyme gene from fenneropenaeus indicus was cloned, expressed in escherichia coli and characterized. the cdna consists of 477 base pairs and encodes amino acid sequence of 159 residues. f. indicus lysozyme had high identity (98%) with fenneropenaeus merguiensis and fenneropenaeus chinensis and exhibits low to moderate identities with lysozymes of other invertebrates and vertebrates. this lysozyme is presumed to be chicken types as it possesses two catalytic and eight cysteine residues that are ... | 2014 | 24676722 |
| immune modulations and protection by translationally controlled tumor protein (tctp) in fenneropenaeus indicus harboring white spot syndrome virus infection. | fenneropenaeus indicus translationally controlled tumor protein (fi-tctp) was cloned and expressed using pet 100a-d-topo in prokaryotic expression system and it exhibited putative antioxidant activity as assessed in vitro by enhanced growth of escherichia coli (e. coli) in presence of hydrogen peroxide. the protective efficacy of recombinant fi-tctp (rfi-tctp) was evaluated in f. indicus by intramuscular and oral administration. intramuscular injection of rfi-tctp to shrimps, on subsequent white ... | 2014 | 24837973 |
| quorum-quenching activity of the ahl-lactonase from bacillus licheniformis dahb1 inhibits vibrio biofilm formation in vitro and reduces shrimp intestinal colonisation and mortality. | vibrio parahaemolyticus is a significant cause of gastroenteritis resulting from the consumption of undercooked sea foods and often cause significant infections in shrimp aquaculture. vibrio virulence is associated with biofilm formation and is regulated by n-acylated homoserine lactone (ahl)-mediated quorum sensing. in an attempt to reduce vibrio colonisation of shrimps and mortality, we screened native intestinal bacilli from indian white shrimps (fenneropenaeus indicus) for an isolate which s ... | 2014 | 25060960 |
| cdna cloning, characterization and expression analysis of a novel antimicrobial peptide gene penaeidin-3 (fi-pen3) from the haemocytes of indian white shrimp fenneropenaeus indicus. | a new member of antimicrobial peptide genes of the penaeidin family, penaeidin 3, was cloned from the haemocytes of indian white shrimp fenneropeneaus indicus (f. indicus), by reverse transcription pcr (rt-pcr) and rapid amplification of cdna end (race-pcr) methods. the complete nucleotide sequence of cdna clone of indian white shrimp f. indicus penaeidin 3 (fi-pen3) was 243bp long and has an open reading frame which encodes 80 amino acid peptide. the homology analysis of fi-pen3 sequence with o ... | 2011 | 21885268 |
| trace elements in edible shellfish species from the lower gangetic delta. | the accumulation of trace elements in edible shellfish species may lead to serious health problems through the food chain. in this study we measured the concentrations of zn, cu, pb and cd in the shellfish species like penaeus monodon, penaeus indicus, scylla serrata, saccostrea cucullata and crassostrea madrasensis using a icp mass spectrometer. samples were collected from the lower stretch of the river ganga which receives wastes of complex character from industries and various anthropogenic a ... | 2011 | 21570722 |
| profiling of a few immune responsive genes expressed in postlarvae of fenneropenaeus indicus challenged with vibrio harveyi d3. | we identified 38 genes and eight hypothetical proteins by sequencing of 1200 clones from a vibrio harveyi challenged fenneropenaeus indicus subtracted cdna library. based on physiological roles and functions these genes were categorized into 10 groups with ∼29% of the sequences having no matches in the databases. immune related transcripts in the library were carboxypeptidase b, ferritin, translationally controlled tumor proteins (tctp), hemocyanin, chitinase and serine carboxy peptidase. remark ... | 2011 | 21515280 |
| differential gene expression profile of the hepatopancreas of white spot syndrome virus infected fenneropenaeus indicus by suppression subtractive hybridization. | suppression subtractive hybridization was employed in order to identify the differentially expressed genes in the hepatopancreas of white spot syndrome virus infected fenneropenaeus indicus. a forward subtracted cdna library generated 356 clones following a white spot syndrome virus infection. a total of 345 clones with more than 100 nucleotides were selected for further analysis using bioinformatics tools after vector screening. twenty-three contigs and 111 singletons were generated from a tota ... | 2010 | 20688172 |
| occurrence of toxigenic vibrio parahaemolyticus strains in shrimp in iran. | vibrio parahaemolyticus, a common cause of foodborne gastroenteritis in people, is frequently isolated from a variety of seafood, including shrimp. the virulence of clinical v. parahaemolyticus strains is commonly associated with expression of thermostable direct hemolysin (tdh) and tdh-related hemolysin (trh), which are encoded by the tdh and trh genes. this study was conducted to determine the prevalence rate of total and toxigenic v. parahaemolyticus in shrimp caught off the south coast of ir ... | 2010 | 20528175 |
| antibacterial activity of silver nanoparticles (agnps) synthesized by tea leaf extracts against pathogenic vibrio harveyi and its protective efficacy on juvenile feneropenaeus indicus. | to determine the antibacterial potential of silver nanoparticles (agnps) synthesized by tea leaf extract against vibrio harveyi and its protective effect on juvenile feneropenaeus indicus. | 2010 | 20132435 |
| purification and characterization of phocaecin pi80: an anti-listerial bacteriocin produced by streptococcus phocae pi80 isolated from the gut of peneaus indicus (indian white shrimp). | a bacteriocin-producing strain pi80 was isolated from the gut of penaeus indicus (indian white shrimp) and identified as streptococcus phocae pi80. the bacteriocin was purified from a culture supernatant to homogeneity as confirmed by tricine sds-page. reverse-phase hplc analysis revealed a single active fraction eluted at 12.94 min, and maldi-tof mass spectrometry analysis showed the molecular mass to be 9.244 kda. this molecular mass does not correspond to previously described streptococcal ba ... | 2009 | 19996693 |
| pathological changes in fenneropenaeus indicus experimentally infected with white spot virus and virus morphogenesis. | to demonstrate pathological changes due to white spot virus infection in fenneropenaeus indicus, a batch of hatchery bred quarantined animals was experimentally infected with the virus. organs such as gills, foregut, mid-gut, hindgut, nerve, eye, heart, ovary and integument were examined by light and electron microscopy. histopathological analyses revealed changes hitherto not reported in f. indicus such as lesions to the internal folding of gut resulted in syncytial mass sloughed off into lumen ... | 2009 | 19686757 |
| media preparation using tuna-processing wastes for improved lipase production by shrimp gut isolate staphylococcus epidermidis cmst pi 2. | extracellular lipase production by staphylococcus epidermidis cmst pi 2 isolated from the intestine of shrimp penaeus indicus has been investigated in shake-flask experiment using different preparations of tuna-processing waste such as raw fish meat, defatted fish meat, alkali hydrolysate, and acid hydrolysate as nitrogen source. among the tested tuna preparations, defatted fish meat supported the maximum lipase production, and 2.5% concentration of the same was found to be optimum for maximizin ... | 2010 | 19430738 |
| identification of immune cells interacting with vibrio spp. and its in vitro post-phagocytic killing mechanism of haemocytes in the penaeid shrimp, penaeus indicus h. milne edwards. | immune cells were identified and their interaction towards vibrio alginolyticus, v. parahaemolyticus and v. anguillarum was studied in vitro in the penaeid shrimp, penaeus indicus. haemocytes were divided into agranulocytes, semi-dense granulocytes and dense granulocytes according to their morphology. agranulocytes (100%) and 0.3-0.7% of granulocytes were actively involved in coagulation. granulocytes were involved in in vitro phagocytosis and encapsulation of foreign materials. phagocytosis was ... | 2009 | 19335612 |
| quantitative and qualitative studies on the bacteriological quality of indian white shrimp (penaeus indicus) stored in dry ice. | indian white shrimp (penaeus indicus) stored in dry ice at the 1:1 ratio were found to be organoleptically suitable for consumption when they were stored for 24 h without reicing. shrimp stored in water ice at the 1:1 ratio (as control) were acceptable up to 18 h. shrimp stored in a combination of dry ice and water ice at the ratio of 1:0.2:0.5 were also found to be acceptable up to 24 h. total bacterial load ranged from 10(6) to 10(9) cfu g(-1), while total psychrophiles ranged from 10(3) to 10 ... | 2006 | 16943047 |
| stability of chloramphenicol residues in shrimp subjected to heat processing treatments. | the stability of chloramphenicol residues in white shrimp (penaeus indicus) subjected to cooking (100 degrees c) for 10, 20 and 30 min (c1, c2 and c3) as well as retorting (121 degrees c) for 10 and 15 min (r1 and r2) was studied by a microbial assay method using photobacterium leiognathi as the test organism. the microbial assay method was found to have a good sensitivity of 1 microg/ml the loss of chloramphenicol in shrimp subjected to cooking for 10, 20 and 30 min was 6%, 12% and 29%, respect ... | 2006 | 16942985 |
| fenneropenaeus indicus is protected from white spot disease by oral administration of inactivated white spot syndrome virus. | fenneropenaeus indicus could be protected from white spot disease (wsd) caused by white spot syndrome virus (wssv) using a formalin-inactivated viral preparation (ivp) derived from wssv-infected shrimp tissue. the lowest test quantity of lyophilized ivp coated onto feed at 0.025 g(-1) (dry weight) and administered at a rate of 0.035 g feed g(-1) body weight d(-1) for 7 consecutive days was sufficient to provide protection from wsd for a short period (10 d after cessation of ivp administration). ... | 2005 | 16261942 |
| n-hexanoyl-l-homoserine lactone-degrading pseudomonas aeruginosa psdahp1 protects zebrafish against vibrio parahaemolyticus infection. | four strains of n-hexanoyl-l-homoserine lactone (ahl)-degrading pseudomonas spp., named psdahp1, psdahp2, psdahp3, and psdahp4 were isolated and identified from the intestine of fenneropenaeus indicus. psdahp1 showed the highest ahl-degrading activity among the four isolates. psdahp1 inhibited biofilm-forming exopolysaccharide and altered cell surface hydrophobicity of virulent green fluorescent protein (gfp)-tagged vibrio parahaemolyticus dahv2 (gfp-vpdahv2). oral administration of psdahp1 sign ... | 2015 | 25449384 |
| development and characterization of eighty-one microsatellite markers in indian white shrimp, fenneropenaeus indicus, through cross-amplification. | | 2015 | 26419436 |
| effect of high pressure treatment on microbiological quality of indian white prawn (fenneropenaeus indicus) during chilled storage. | high pressure treatment of 250 mpa for 6 min at 25 °c was applied to headless indian white prawn (fenneropenaeus indicus) to evaluate changes in microbiological characteristics of the species during chilled storage. changes in load of mesophilic bacteria, psychrotrophic bacteria, proteolytic bacteria, enterobacteriaceae, pseudomonas spp., h2s producing bacteria, lactic acid bacteria, brochothrix thermosphacta and yeast & mold were estimated in pressurized and un-pressurized samples during chille ... | 2015 | 25475333 |
| homology modeling, molecular dynamics, and docking studies of pattern-recognition transmembrane protein-lipopolysaccharide and β-1,3 glucan-binding protein from fenneropenaeus indicus. | lipopolysaccharide and β-1,3 glucan-binding protein (lgbp) is a family of pattern-recognition transmembrane proteins (prps) which plays a vital role in the immune mechanism of crustaceans in adverse conditions. fenneropenaeus indicus lgbp-deduced amino acid has conserved potential recognition motif for β-1,3 linkages of polysaccharides and putative rgd (arg-gly-asp) cell adhesion sites for the activation of innate defense mechanism. in order to understand the stimulating activity of β-1,3 glucan ... | 2015 | 25139673 |
| antioxidative activity of protein hydrolysate produced by alcalase hydrolysis from shrimp waste (penaeus monodon and penaeus indicus). | protein hydrolysates prepared by hydrolysis of shrimp waste (penaeus monodon and penaeus indicus) for 90 min. using alcalase enzyme following ph-stat method. antioxidative activities of swph were assessed determining frap, abts and dpph radical scavenging activities, which increased linearly with increasing concentration of protein hydrolysate upto 5 mg/ml maintaining good correlation. swph showed high stability over wide ranges of ph (2-11) and temperature (up to 100 °c for 150 min), in which t ... | 2014 | 24587519 |
| new genotypes of white spot syndrome virus (wssv) and taura syndrome virus (tsv) from the kingdom of saudi arabia. | white spot syndrome virus (wssv) and taura syndrome virus (tsv) are highly pathogenic to penaeid shrimp and have caused significant economic losses in the shrimp culture industry around the world. during 2010 and 2011, both wssv and tsv were found in saudi arabia, where they caused severe mortalities in cultured indian white shrimp penaeus indicus. most outbreaks of shrimp viruses in production facilities can be traced to the importation of infected stocks or commodity shrimp. in an attempt to d ... | 2012 | 22832716 |
| genome sequence of vibrio parahaemolyticus vp103 strain isolated from shrimp in malaysia. | | 2016 | 27708636 |
| genome sequence of vibrio parahaemolyticus vp152 strain isolated from penaeus indicus in malaysia. | | 2016 | 27656174 |
| essential oils of nigella sativa protects artemia from the pathogenic effect of vibrio parahaemolyticus dahv2. | the anti-vibrio activity of essential oils (eos) of nine medicinal plants was tested against 28 vibrio spp. isolated from diseased fenneropenaeus indicus. eo of nigella sativa exhibited anti-vibrio activity against all vibrio spp. and greater inhibition was noted for the isolate v2 which was identified as vibrio parahaemolyticus dahv2. further, eo of n. sativa effectively inhibited v. parahaemolyticus dahv2 with an inhibition zone of 23.9mm at 101.2μgml(-1). moreover, eo of n. sativa revealed an ... | 2016 | 26945773 |
| cdna cloning, characterization and expression of lipopolysaccharide and β-1,3-glucan binding protein (lgbp) gene from the indian white shrimp fenneropenaeus indicus. | a 1372bp full length cdna of an lgbp gene was identified from the indian white shrimp, fenneropenaeus indicus. the open reading frame (orf) of the f. indicus lgbp (fein-lgbp) was 1164bp long, encoding a polypeptide of 388 amino acids. the fein-lgbp deduced amino acid has conserved potential recognition motif for β-1, 3 linkages of polysaccharides and putative rgd (arg-gly-asp) cell adhesion sites. phylogenetic analysis revealed that the fein-lgbp was grouped together with lgbps of other crustace ... | 2012 | 22609928 |
| molecular cloning, sequence analysis and expression of fein-penaeidin from the haemocytes of indian white shrimp fenneropenaeus indicus. | penaeidins are members of a special family of antimicrobial peptide existing in penaeid shrimp and play an important role in the immunological defense of shrimp. here, we report a penaeidin sequence cloned from the indian white shrimp fenneropenaus indicus (fein-penaeidin). the fein-penaeidin open reading frame encodes a 77 amino acid peptide including a 19 amino acid signal peptide. the deduced amino acid sequences of fein-penaeidin include a proline rich n-terminal domain and a carboxyl-domain ... | 2012 | 24371565 |
| response of glutathione system and carotenoids to sublethal copper in the postlarvae of penaeus indicus. | the objective of this study is to determine the effect of sublethal copper on the glutathione system and carotenoids of penaeus indicus postlarvae (pl) when subjected to short- and long-term exposure in the laboratory. the pl were exposed to 0.1641ppm (sublethal) copper for a period of 30 days with sampling intervals of 24, 48, 96h and 10, 20, 30 days. variations in the activity of glutathione reductase (gr), glutathione peroxidase (gpx) and glutathione-s-transferase (gstase) were measured as bi ... | 2012 | 21944956 |
| a novel clip domain serine proteinase (sps) gene from the haemocytes of indian white shrimp fenneropenaeus indicus: molecular cloning, characterization and expression analysis. | | 2011 | 21300157 |
| molecular characterization of a crustin-like, putative antimicrobial peptide, fi-crustin, from the indian white shrimp, fenneropenaeus indicus. | | 2010 | 19837171 |
| fishmicrosat: a microsatellite database of commercially important fishes and shellfishes of the indian subcontinent. | microsatellite dna is one of many powerful genetic markers used for the construction of genetic linkage maps and the study of population genetics. the biological databases in public domain hold vast numbers of microsatellite sequences for many organisms including fishes. the microsatellite data available in these data sources were extracted and managed into a database that facilitates sequences analysis and browsing relevant information. the system also helps to design primer sequences for flank ... | 2013 | 24047532 |
| seasonal abundance and composition of finfish and shellfish seeds inmangroves of gangolli estuary, off south-west coast of india. | the composition and seasonal abundance of the fin and shellfish seeds in mangrove areas of gangoli estuary were studied for one year from january to december, 2013. monthly day time sampling was conducted with a rectangular dragnet of 75 m2 length having 5 mm mesh size. the mean monthly density was highest in january (avg. of 12,996/haul) and lowest during may (avg. of 859/haul). thirty species of fish, four species of shrimps and two species of crabs belonging to 26 families were recorded durin ... | 2015 | 26688975 |
| histological and immunocytochemical localization of serotonin-like immunoreactivity in the brain and optic ganglia of the indian white shrimp, fenneropenaeus indicus. | serotonin is one of the important neurotransmitter and neuromodulator so far studied in crustacean models. with its secretory sites well-studied in higher crustaceans, its function in controlling the release of metabolic hormones from their storage and release sites has been well proved. the present study attempts to localize serotonin-like immunoreactivity in fenneropenaeus indicus, a commercially important shrimp species and a natural inhabitant of the indian oceans. histological studies were ... | 2008 | 17661386 |
| fast gas chromatography with tandem mass spectrometry analysis of selected persistent organic pollutants and organophosphorus and synthetic pyrethroid pesticides in indian prawn (fenneropenaeus indicus) in compliance with the eu-mrls. | a fast gc with tandem ms method was developed and validated for multiresidue determination of 95 chemical contaminants(24 synthetic pyrethroids, 17 organochlorines, 17 organophosphorus compounds, 18 polycyclic aromatic hydrocarbons, and 19 polychlorinated biphenyls) in indian prawns (<i>fenneropenaeus indicus</i>) as per the european union maximum residual limit requirements. chromatographic separation and ms determination were achieved within a short run time of 18 min, without compromising sen ... | 2017 | 28303785 |
| haemolytic and antibiofilm properties of haemocyanin purified from the haemolymph of indian white shrimp fenneropenaeus indicus. | haemocyanin (hc) is an important non-specific immune macromolecule present in the haemolymph of both mollusks and crustaceans. in the present study, hc was purified from the haemolymph of indian white shrimp fenneropenaeus indicus by gel filtration chromatography and it exhibits a single band with a molecular weight of 74 kda on sds-page. the x-ray diffraction (xrd) and high performance liquid chromatography (hplc) result of purified hc express single peak at 31.5° be a sign of crystalline natur ... | 2016 | 27815202 |
| energy levels of penaeus indicus postlarvae on exposure to lead. | in the present investigation the changes in energy levels or caloric concentrations of penaeus indicus postlarvae (pl) were determined after exposure to acute and sublethal concen- trations of lead. the biochemical constituents such as carbo- hydrates, proteins, and lipids were estimated in pl exposed to lc(5) (0.1495), lc(10) (0.3488), lc(25) (1.4660), and lc(50) (7.223 ppm) of lead for 48h. the effect of sublethal concentration (1.44 ppm) of lead was also studied on the above biochemical const ... | 2002 | 12297076 |
| a novel l-fucose-binding lectin from fenneropenaeus indicus induced cytotoxicity in breast cancer cells. | lectins are omnipresent in almost all life forms, being the proteins which specifically bind to carbohydrate moieties on the cell surface; they have been explored for their anti-tumour activities. in this study, we purified a fucose specific-lectin (ifl) from fenneropenaeus indicus haemolymph using fucose-affinity column and characterized for its haemagglutination activity, carbohydrate specificity, dependency on cations and cytotoxicity against cancer cells. the lectin showed non-specificity ag ... | 2017 | 27742744 |
| environmental monitoring of pesticide exposure and effects on mangrove aquatic organisms of mozambique. | the use of pesticides in mozambique is increasing along with the development of agriculture in the country. mangroves along the coastlines are ecologically important areas and vital nursing grounds for many aquatic species, several of which are of high economic value in mozambique. barred mudskipper (periophthalmus argentilineatus), jarbua fish (terapon jarbua), indian white prawn (penaeus indicus) and the clam meretrix meretrix were collected at three mangrove sites in the maputo bay area. this ... | 2016 | 27422103 |
| the effects of different routes of inulin administration on gut microbiota and survival rate of indian white shrimp post-larvae (fenneropenaeus indicus). | the present study investigates the effects of different routes of inulin administration as prebiotic on gut microbiota and survival rate of indian white shrimp post-larvae. four hundred and fifty indian white shrimp post-larvae (pl1) were stocked in nine tanks. the tanks were assigned into three treatments: feeding with inulin-treated (110 mg l(-1)) artemia nauplii (i-t), feeding with inulin-enriched (110 mg l(-1)) artemia nauplii (i-e) and control which repeated triplicates. feeding trial was p ... | 2015 | 26973770 |
| larval growth in postlarvae of penaeus indicus on exposure to lead. | | 2001 | 11381308 |
| in vitro and in silico studies on cell adhesion protein peroxinectin from fenneropenaeus indicus and screening of heme blockers against activity. | in invertebrates, the prophenoloxidase (propo) pathway is involved in the phenol-like antioxidant production against invading pathogens. overproduction of melanin and phenolic substances leads to the disruption of hemocytes (own host cells); therefore, there is a prerequisite to regulate the antioxidant production, which is performed by the proteases and propo-associated cell adhesion protein peroxinectin (px). px is a macromolecular structure consisting of protein involved in the propo pathway, ... | 2016 | 26686319 |
| biopreservation of sardinella longiceps and penaeus monodon using protective culture streptococcus phocae pi 80 isolated from marine shrimp penaeus indicus. | the influence of streptococcus phocae pi80 on the shelf life of sardinella longiceps and penaeus monodon was investigated by measurement of microbial and chemical analysis after appraising the safety of the protective probiotic culture in wistar rat's model. the results of this safety assessment indicate that oral administration of protective culture does not demonstrate any toxicological effects. consumption of this lab strain had no adverse effects on animal's general health status, hematology ... | 2011 | 26781574 |
| inhibition of na+,k+-atpase in penaeus indicus postlarvae by lead. | the plasma membrane/mitochondrial fractions of penaeus indicus postlarvae contain mg2+-dependent atpase, na+,k+-stimulated atpase, na+-stimulated atpase and k+-stimulated atpase. the na+,k+-activated, mg2+-dependent atpase was investigated further in relation to different ph and temperature conditions, and at various concentrations of protein, ouabain, atp and ions in the incubation medium. in vitro and in vivo effects of lead were studied on the enzyme activity. in vitro lead inhibited the enzy ... | 2000 | 11081409 |
| oxygen consumption, ammonia-n excretion, and metal accumulation in penaeus indicus postlarvae exposed to lead. | | 2000 | 10606706 |
| effect of hydrogen sulphide on two species of penaeid prawns penaeus indicus (h. milne edwards) and metapenaeus dobsoni (miers). | | 1996 | 8791560 |
| molecular characterization of a newly identified subfamily member of penaeidin from two penaeid shrimps, fenneropenaeus indicus and metapenaeus monoceros. | penaeidins are a major group of antimicrobial peptides found in penaeid shrimps. this study reports a new isoform of penaeidin from the hemocytes of indian white shrimp, fenneropenaeus indicus (fi-pen, jx657680), and the pink shrimp, metapenaeus monoceros (mm-pen, kf275674). mm-pen is also the first antimicrobial peptide to be identified from m. monoceros. the complete coding sequences of the newly identified fi-pen and mm-pen consisted of an orf of 338 bp encoding 71 amino acids with a predicte ... | 2016 | 26607699 |
| white spot syndrome virus infection: threat to crustacean biodiversity in vembanad lake, india. | the vembanad lake located on the south-west coast of india, an ecological hotspot is the nursing ground of many economically important crustaceans. the prevalence of white spot syndrome virus (wssv) among crustaceans from farmed, estuarine and marine environments surrounding the vembanad lake, india was detected using pcr. a total of 308 samples from aquaculture ponds consisting of six species of crustaceans collected from five different farms were tested for the presence of wssv. of these, 67% ... | 2015 | 28352566 |
| monoclonal antibody based immunodot for specific detection of proteins of the shrimp penaeus species. | frozen shrimp continued to be the single largest item of export from india in terms of value accounting for about 44% of the total marine export earnings. headless, peeled frozen shrimp is a common and dominant item in the market and there is need for differentiating peeled penaeus sp from metapenaeus, parapenopsis and macrobrachium sp as consumer preference and price vary. furthermore, there is need to find out original species used in value addition of shrimp products. hence, it is essential f ... | 2014 | 24803705 |
| erratum to: biopreservation of sardinella longiceps and penaeus monodon using protective culture streptococcus phocae pi 80 isolated from marine shrimp penaeus indicus. | | 2011 | 26781575 |
| influence of agathi grandiflora active principles inhibit viral multiplication and stimulate immune system in indian white shrimp fenneropenaeus indicus against white spot syndrome virus infection. | five herbs including adathoda vasica, agathi grandiflora, leucas aspera, psoralea corylifolia, and quercus infectoria were selected to screen the antiviral and immunostimulant activity against white spot syndrome virus (wssv) and vibrio harveyi respectively using different organic polar and non-polar solvents. based on the initial screening results, ethyl acetate and methanolic extracts of a. grandiflora had strong antiviral and immunostimulant activities. those extracts incubated with wssv inje ... | 2014 | 25301717 |
| molecular cloning of peroxinectin gene and its expression in response to peptidoglycan and vibrio harveyi in indian white shrimp fenneropenaeus indicus. | the cdna sequence of peroxinectin was obtained from the haemocytes of indian white shrimp fenneropenaeus indicus using rt-pcr and race. fenneropenaeus indicus peroxinectin (fi-pxn) sequence has an open reading frame (orf) of 2415 bp encoding a protein of 804 amino acids with 21 residues signal sequence. the mature protein has molecular mass of 89.8 kda with an estimated pi of 8.6. two putative integrin-binding motifs, rgd and kgd, were observed at the basic n-terminal and c-terminal part of the ... | 2014 | 25072536 |
| modulation of carbohydrate metabolism in the selected tissues of marine prawn, penaeus indicus (h. milne edwards), under phosphamidon-induced stress. | changes in carbohydrate metabolism were studied in midgut gland, muscle, and gill tissues of marine prawn penaeus indicus exposed to a sublethal concentration (0.3 ppm) of phosphamidon. a significant decrease in glycogen and pyruvate and an increase in lactate content were observed in all phosphamidon-exposed prawn tissues after 96 hr. an increase in phosphorylase a and aldolase activity levels suggested the increased formation of triose sugars during phosphamidon toxicity. ldh activity was cons ... | 1988 | 3378538 |
| full-length cloning and phylogenetic analyses of translationally controlled tumour protein and ferritin genes from the indian white prawn, fenneropenaeus indicus (h. milne edwards). | elucidation, through molecular analyses, of bacterial afflictions in commercially important aquaculture-reared shrimps is pivotal for the prevention and/or control of disease outbreaks. in this study, we examined the phylogenetic relatedness and compared the possible immune-related functional roles of both translationally controlled tumour protein (tctp) and ferritin genes with previous studies. both tctp and ferritin genes were substantially upregulated in the indian white prawn, fenneropenaeus ... | 2014 | 24329985 |
| influence of acute salinity changes on biochemical, hematological and immune characteristics of fenneropenaeus indicus during white spot syndrome virus challenge. | the present study reports the influence of salinity (5, 15, 25 and 35 g/l) on the biochemical and immune characteristics of fenneropenaeus indicus challenged with 5. 5 × 10(4) copy number of white spot syndrome virus (wssv). f. indicus that had been reared in 25 g/l, injected with wssv and transferred to 5, 15, 25 (control) and 35 g/l were examined after 0-120 hrs for total hemocyte count (thc), phenoloxidase (po) and respiratory burst (rb) activity and alkaline and acid phosphatase activities. ... | 2013 | 23773025 |
| influence of acute salinity changes on biochemical, hematological and immune indices of fenneropenaeus indicus during white spot syndrome virus (wssv) challenges. | the present study reports the influence of salinity (5, 15, 25 and 35 g/l) on the biochemical and immune parameters of fenneropenaeus indicus challenged with 5. 5 × 10(4) copy number of white spot syndrome virus. f. indicus that were reared in 25 g/l, were injected with wssv, and then transferred to 5, 15, 25 (control) and 35g/l were examined for total haemocyte count (thc), phenoloxidase (po) activity, superoxide dismutase (sod) activity, alkaline phosphatase assay and acid phosphatase assay af ... | 2013 | 23586689 |
| a histological variant of white spot syndrome virus (wssv) from the kingdom of saudi arabia. | white spot syndrome virus (wssv) is highly pathogenic to penaeid shrimp. the major targets of wssv infection are tissues of ectodermal and mesodermal embryonic origin, predominantly the cuticular epithelium and subcuticular connective tissues. recently, we discovered a wssv variant in penaeus indicus that heavily infects the subcuticular connective tissue, with very slight indications in the cuticular epithelium. the variant was also unusual in that wssv accumulations were found in the interstit ... | 2013 | 23454062 |
| heavy metals accumulation in crab and shrimps from pulicat lake, north chennai coastal region, southeast coast of india. | the accumulation of heavy metals such as lead (pb), iron (fe), zinc (zn), cadmium (cd), and chromium (cr) was examined in crab (scylla serrata) and shrimps (penaeus semisulcatus, penaeus indicus, and penaeus monodon) collected from pulicat lake that receives effluents from industries located in north chennai, southeast coast of india. the results showed limited difference between crab and prawns as well as significant variations between the organs. pb is the highly accumulated metal in both crab ... | 2016 | 23344825 |
| temperature effect on behaviour, oxygen consumption, ammonia excretion and tolerance limit of the post larvae of shrimp penaeus indicus. | the present study has been carried out to know the effect of temperature on behaviour, equilibrium loss and tolerance limit of the post larvae of shrimp penaeus indicus. the experimental temperatures were selected based on the thermal tolerance limit. the experiments were conducted at a specific temperature for duration of 48 hr. the thermal tolerance experiments were conducted in two ways: in direct exposure and in gradually increasing temperature. the upper and lower lethal temperatures for th ... | 2008 | 19192924 |
| purification and ige-binding epitopes of a major allergen in the gastropod turbo cornutus. | the major allergen (tur c 1) in the muscle of the gastropod, turbo cornutus, was isolated by sephacryl s-300, mono q hr 5/5 and tskgel phenyl-5pw rp column chromatography. elisa showed tur c 1 to react strongly with sera from three individuals sensitive to both mollusks and crustaceans. sds-page showed tur c 1 to produce a major band corresponding to a molecular mass of 35 kda under the reduced condition. its amino acid composition was characterized by the abundance of glx, followed by leu, ala ... | 1998 | 9720216 |
| notice of retraction: localization of serotonin neuropiles in the brain and thoracic ganglia of the indian white shrimp, fenneropenaeus indicus: phylogenetic comparisons and implications for arthropod relationships. | | 2008 | 18431791 |
| molecular cloning, expression analysis and characterization of peroxiredoxin during wssv infection in shrimp fenneropenaeus indicus. | innate immunity is the first line of defense in shrimps against invading pathogenic microorganisms. peroxiredoxins (prx) are the family of antioxidant proteins that play a crucial role in reduction of oxidative stress in host during viral infection. peroxiredoxin from fenneropenaeus indicus was identified, cloned and expressed in prokaryotic expression system. the fi-prx protein consists of two conserved cys residues and belongs to typical 2-cys prx family. the phylogenetic analysis revealed a c ... | 2012 | 21964313 |
| cadmium uptake and accumulation by the decapod crustacean penaeus indicus. | juveniles of the dendrobranchiate decapod penaeus indicus take up radiolabelled cadmium from solution over the exposure concentration range of 1.8-31.5 microg l(-1), with an uptake rate constant of 0.090 l g(-1)d(-1) at 15 salinity and 25 degrees c. new cadmium taken up is added to the existing cadmium content of the prawn with no significant excretion, and the rate of accumulation of radiolabelled cadmium is a measure of the absolute cadmium uptake rate from solution. moulting had no significan ... | 2005 | 15769503 |
| effect of lead on na+, k(+)-atpase activity in penaeus indicus postlarvae. | in vivo effect of lead on na, k(+)-atpase was studied in plasma membrane/mitochondrial fraction of p. indicus post-larvae (pl), exposed to 30 days to a sublethal concentration (1.44 ppm) of lead. a significant (p < 0.05) decrease in the enzyme activity was observed for exposed pl with respect to their controls at different intervals except 24hr. further the substrate (atp) and ion (na+ and k+)-dependent kinetics of na+, k(+)-atpase was studied with the plasma membrane/mitochondrial fractions of ... | 2002 | 12597561 |
| acute toxicity of lead on tolerance, oxygen consumption, ammonia-n excretion, and metal accumulation in penaeus indicus postlarvae. | the estuaries and backwaters that are the potential breeding grounds of penaeid shrimps are subject to heavy metal pollution through industrial effluents and domestic sewage. in the present investigation, laboratory experiments were conducted to study the acute toxicity of lead on tolerance, oxygen consumption, ammonia-n excretion, and metal accumulation in penaeus indicus postlarvae. static bioassay tests were employed to determine tolerance limits. oxygen consumption, ammonia-n excretion, and ... | 2002 | 11886180 |
| evidence for a hyperglycemic effect of methionine-enkephalin in the prawns penaeus indicus and metapenaeus monocerus. | the influence of methionine-enkephalin on carbohydrate metabolism of the prawns penaeus indicus and metapenaeus monocerus was studied. injection of the opioid methionine-enkephalin into intact prawns induced significant hyperglycemia in a dose-dependent manner. total tissue (midgut gland and muscle) carbohydrate and glycogen levels decreased following methionine-enkephalin injection, with a significant activation of phosphorylase in intact prawns, indicating glycogenolysis leading to hyperglycem ... | 2001 | 11551121 |
| purification and characterization of a natural agglutinin in the hemolymph of the prawn penaeus indicus h. milne edwards. | a natural agglutinin in the hemolymph of the marine prawn penaeus indicus was isolated by gel filtration chromatography, purified using polyacrylamide gel electrophoresis, and characterized. prawn agglutinin has a native molecular mass of 181 kda and consists of two monomeric units (97 and 84 kda), maintains some agglutinating activity over a wide ph range (7-9), and is inactivated at 85 degrees c. the agglutinin was denatured upon mixing with trichloroacetic acid, phenol, chloroform, and 45% am ... | 2001 | 11437526 |
| studies on digestive proteases from midgut glands of a shrimp, penaeus indicus, and a lobster, nephrops norvegicus: part 1. proteolytic activity. | digestive gland protease ph optima and specific activities determined in penaeus indicus with casein, azocasein, azocoll, and congo red fibrin as substrates were ph 7.7-9.2, 210-371 micromol of tyrosine/mg of homogenate protein/min; ph 7.8, 36; ph 6.0-7.0, 7; and ph 8.9-9.2, 7a delta0.001 u/mg of homogenate protein/min, respectively. activity in the shrimp was stable during frozen storage but relatively labile and very low (1.043 azocasein units) in the norwegian lobster, nephrops norvegicus. th ... | 2001 | 11297389 |
| involvement of opioid peptides in the regulation of reproduction in the prawn penaeus indicus. | the possible involvement of an endogenous opioid system in the regulation of ovarian development in the prawn penaeus indicus was investigated. injection of leucine-enkephalin significantly increased the ovarian index and oocyte diameter in a dose-dependent manner. in contrast, injection of methionine-enkephalin significantly decreased the ovarian index and oocyte diameters. these results provide evidence to support the hypothesis that an opioid system is involved in the regulation of reproducti ... | 2000 | 11198193 |
| neuropeptides and related nucleic acid sequences detected in peneid shrimps by immunohistochemistry and molecular hybridizations. | the neurosecretory cells in the eyestalks of penaeus indicus and p. vannamei were studied by immunocytochemistry using polyclonal antisera raised against purified homarus americanus neuropeptides. cross-reactions between two h. americanus anti-crustacean hyperglycemic hormone antisera and penaeus neurosecretory material were observed. the specific anti-vitellogenesis inhibiting hormone antiserum only showed an immunological reaction in the nervous tract and the sinus gland of penaeus, suggesting ... | 1994 | 8072696 |
| subacute physiological stress induced by phosphamidon on carbohydrate metabolism in midgut gland of prawn, penaeus indicus. | midgut gland carbohydrate metabolism of penaeid prawn, penaeus indicus was studied on acute and acclimation to sublethal concentration of phosphamidon. the midgut gland tissue of acclimated prawn showed inhibited glycolysis with an onset of gluconeogenesis. in general acclimation to sublethal concentration resulted in the elevation of the synthetic phase of midgut gland carbohydrate metabolism. | 1991 | 1877989 |
| methylparathion, carbaryl and aldrin impact on nitrogen metabolism of prawn, penaeus indicus. | changes in hepatopancreas, muscle and gill tissue nitrogen metabolic profiles were studied in a penaeid prawn, penaeus indicus, following its exposure to sublethal concentrations of methylparathion, carbaryl and aldrin. in all the insecticide exposed prawn tissues, ammonia levels were significantly increased and a shift in the nitrogen metabolism towards the synthesis of urea and glutamine was observed. inhibition of glutamate oxidation to ammonia and alpha-ketoglutarate by glutamate dehydrogena ... | 1991 | 1907140 |
| toxic impact of lindane on aspartate and lindane aminotransferase activity levels of midgut gland of marine prawn, penaeus indicus. | in vitro addition of lindane (organochlorine insecticide) causing significant changes in the protein and amino acid metabolic profiles of midgut gland by interfering with the aminotransferase system of penaeus indicus. | 1991 | 1713456 |
| exploration of protein-protein interaction effects on α-2-macroglobulin in an inhibition of serine protease through gene expression and molecular simulations studies. | in prophenoloxidase (propo) cascade, two targets namely serine protease and α-2-macroglobulin are key regulators involved in the defense system of crustaceans. in biological systems, routine role of cell systems requires the understanding in protein-protein interactions through experimental and theoretical concepts, which might yield useful insights into the cellular responses. response of cells to regulating the immune system is governed by the interactions-involved biomolecular simulations. un ... | 2014 | 24124927 |
| toxic impact of phosphamidon on protein degradation in phasic and tonic muscles of marine prawn, penaeus indicus (h. milne edwards). | levels of various protein fractions, (sarcoplasmic, myosin, actin, non-collagen and collagen) and the rate of their degradation by proteases were studied in phasic and tonic muscles of marine prawn, penaeus indicus following acute (2 d) and chronic (15 d) exposure to sublethal concentration of phosphamidon. during exposure, greater decrease in sarcoplasmic protein fraction was observed in phasic muscle as compared to other myofibrillar proteins. but the sarcoplasmic protein content showed an ele ... | 1990 | 1705773 |
| mercury contamination of fish and shrimp samples available in markets of mashhad, iran. | fish and shrimp are common healthy sources of protein to a large percentage of the world's population. hence, it is vital to evaluate the content of possible contamination of these marine-foods. six species of fishes and two species of shrimps were collected from the local markets of mashhad, iran. the mercury (hg) concentration of samples was determined by atomic absorption spectrophotometry using a mercuric hydride system (mhs 10). high concentration of total hg was found in clupeonella cultri ... | 2013 | 23903758 |
| changes in nitrogen metabolism of penaeid prawn, penaeus indicus, during sublethal phosphamidon and methylparathion-induced stress. | | 1988 | 3219427 |
| white spot syndrome virus (wssv) genome stability maintained over six passages through three different penaeid shrimp species. | white spot syndrome virus (wssv) replicates rapidly, can be extremely pathogenic and is a common cause of mass mortality in cultured shrimp. variable number tandem repeat (vntr) sequences present in the open reading frame (orf)94, orf125 and orf75 regions of the wssv genome have been used widely as genetic markers in epidemiological studies. however, reports that vntrs might evolve rapidly following even a single transmission through penaeid shrimp or other crustacean hosts have created confusio ... | 2014 | 25144114 |
| physicochemical properties of anti vibrio harveyi egg yolk antibody (igy) and its immunological influence in indian white shrimp fenneropenaeus indicus. | edible antibodies specific to host pathogens is an attractive approach to establish protective immunity, especially against gastrointestinal pathogens both in humans and animals. the edible antibody of anti-vibrio harveyi igy (anti-v. h igy) was produced by antigen mixed with immunoadjuvant asparagus racemosus and glycine max. hens were immunized and eggs were collected five weeks after the immunization. anti-v. harveyi igy stability in different digestive enzymes such as trypsin and chymotrypsi ... | 2018 | 29307631 |