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amino acid analogs iv:4-fluoroisoleucine.4-fluoroisoleucine was produced by ammonolysis of 2-bromo-4-fluoro-3-methylpentanoic acid, which resulted from the bromofluorination of 4-methyl-2-pentenoic acid. it did not inhibit plasmodium berghei in mice at 640 mg/kg and was not toxic to the animals. the fluoroamino acid inhibited aspergillus niger, trichoderma viride, myrothecium verrucaria, trichophyton mentagrophytes, and mucor mucedo in czapek solution agar at a concentration between 10(4) and 10(3) microgram/ml. growth of escherichia ...1978347050
antifungal properties of halofumarate esters.alkyl esters (c1--c4) of the four halofumaric acids were tested for antifungal activity against candida albicans, aspergillus niger, mucor mucedo, and trichophyton mentagrophytes at ph 5.6 and 7.0 in the absence and presence of 10% beef serum in sabouraud dextrose agar. the most toxic compound to each organism was: c. albicans, ethyl iodofumarate (0.054 mmole/liter); a. niger, methyl bromofumarate (0.090 mmole/liter); m. mucedo, methyl fluorofumarate (0.037 mmole/liter); and t. mentagrophytes, e ...1978641780
effect on microorganisms of volatile compounds released from germinating seeds.volatile compounds evolved from germinating seeds of slash pine, bean, cabbage, corn, cucumber, and pea were evaluated for their ability to support growth of microorganisms in liquid mineral salts media lacking a carbon source. growth of eight bacteria was measured turbidimetrically and of six fungi as dry weight of mycelium. volatiles caused increased growth of pseudomonas fluorescens, bacillus cereus, erwinia carotovora, agrobacterium tumefaciens, a. radiobacter, rhizobium japonicum, mucor muc ...19751201509
recovery of metal ions by microfungal filters.many microfungi contain chitin/chitosan as an integral part of the cell wall structure. the binding of toxic and heavy metal ions by chitosan or partly deacetylated chitin is a direct consequence of the base strength of the primary amine group and is most effective for those metals that form complexes with ammonia. of the microfungi studied, hyphae from mucor mucedo and rhizomucor miehei, after treatment with hydroxide to expose the chitin/chitosan, were found to be most effective in the capture ...19901366966
antifungal activity of diethyldithiocarbamate.sodium diethyldithiocarbamate (dtc) was evaluated for its ability to combat four different species of fungi in vitro. using a microtiter-broth-dilution method we were able to demonstrate an antifungal activity against candida albicans, cryptococcus neoformans, aspergillus fumigatus and mucor mucedo in doses achievable by intravenous administration in man.19892555712
[effect of 2-ethyl-2-ene-1-al and analogous compounds on the growth and ultrastructure of fungi].analogs of the natural substance (e)-hex-2-en-1-al which occurs naturally in green plants and is known to have microbiocidal properties were synthesized and studied with respect to their antifungal properties. saturated and unsaturated aldehydes, alcohols, carbon acids and enolacetate were tested against various fungi by applying these compounds either over the gaseous phase or in suspension. the following ranking was observed on the basis of fungicidal activity: enolacetate of 2-ethylhex-2-enoi ...19863097298
acidic polysaccharides from the cell wall of absidia cylindrospora, mucor mucedo, and rhizopus nigricans. 19705471789
antifungal properties of 2-n-alkyn-1-ols.fourteen 2-n-alkynols (c3-c14, c16, and c18) were tested against aspergillus oryzae, aspergillus niger, trichoderma viride, and myrothecium verrucaria in sabouraud dextrose agar at ph 5.6 and 7.0. toxicity to candida albicans, candida tropicalis, trichophyton mentagrophytes, and mucor mucedo was determined in the same medium at ph 5.6 and 7.0 in the absence and presence of 10% beef serum. fungitoxicity was strongly influenced by chain length, slightly by the ph of the medium, and significantly b ...19846098642
cell wall degradation in the autolysis of filamentous fungi.a systematic study on autolysis of the cell walls of fungi has been made on neurospora crassa, botrytis cinerea, polystictus versicolor, aspergillus nidulans, schizophyllum commune, aspergillus niger, and mucor mucedo. during autolysis each fungus produces the necessary lytic enzymes for its autodegradation. from autolyzed cultures of each fungus enzymatic precipitates were obtained. the degree of lysis of the cell walls, obtained from non-autolyzed mycelia, was studied by incubating these cell ...19826219290
deviation from the usual relationships between the temperature, the growth rate, the fatty acid composition and the lipid microviscosity of four different fungi (mucor mucedo, aspergillus ochraceus, scopulariopsis brevicaulis, achlya bisexualis. 19817306120
antifungal properties of n-alkanols, alpha, omega-n-alkanediols, and omega-chloro-alpha-alkanols.fourteen n-alkanols (c1-c12, c14, and c16), 13 alpha,omega-n-alkanediols (c2-c12, c14, and c16), and 13 omega-chloro-alpha-alkanols (c2-c12, c14, and c16) were tested against aspergillus niger, trichoderma viride, and myrothecium verucaria in sabouraud dextrose agar at ph 4.0 and 5.6. toxicity to candida blbicans, trichophyton mentagrophytes, and mucor mucedo was determined in the same medium at ph 5.6 and 7.0 in the absence and presence of 10% beef serum. the fungitoxicity of these alcohols was ...19807373528
isolation and physico-chemical characterization of an antifungal and antibacterial peptide produced by bacillus licheniformis a12.an antifungal substance named peptide a12-c has been purified to homogeneity from supernatants of sporulated cultures of bacillus licheniformis a12. it consists of a 0.77-kda hydrophilic peptide containing two residues of glu and one of arg, ala, pro, tyr and orn. no fatty acids, phosphorus or carbohydrates have been detected. peptide a12-c is active on several fungi (microsporum canis cect 2797, mucor mucedo cect 2653, m. plumbeus (ccm f 443, sporothrix schenckii cect 2799 and trichophyton ment ...19937763922
fungicin m4: a narrow spectrum peptide antibiotic from bacillus licheniformis m-4.the strain bacillus licheniformis m-4 produces a 3.4 kda hydrophilic peptide with antifungal activity, named fungicin m4. analysis of the purified peptide shows that it contains the amino acids glu (8), arg (5), pro (4), tyr (8), val (3), met (2) and orn (4). its inhibitory spectrum is restricted to microsporum canis cect 2797, mucor mucedo cect 2653, mucor plumbeus ccm 443, sporothrix schenckii cect 2799, bacillus megaterium and corynebacterium glutamicum cect 78. fungicin m4 exerts biocidal ac ...19947928782
biocompatibility of potential wound management products: fungal mycelia as a source of chitin/chitosan and their effect on the proliferation of human f1000 fibroblasts in culture.aspergillus oryzae, mucor mucedo, and phycomyces blakesleeanus cultures were examined as sources of chitin/chitosan. the nitrogen content of the alkali-treated mycelia/sporangiophores of a. oryzae, m. mucedo, and p. blakesleeanus was 2.52, 3.61, and 6.27% w/w, which relates to an estimated chitin content of 37, 52, and 91%, respectively. the effect of these fungal materials on the rate of proliferation of human f1000 fibroblasts in culture was examined. at 0.01% w/v, all three materials exhibite ...19948006051
fungi associated with post-harvest rot of black plum (vitex doniana) in nigeria.the fungi associated with rot of vitex doniana fruits (blackplum) were isolated and identified. aspergillus niger, botryodiplodia theobromae, candida spp. penicillium chrysogenum, rhizopus stolonifer, fusarium pallidoroseum f. oxysporum and mucor mucedo were the primary rot causing fungi in contrast to cladosporium herbarum and mucor circinelloides which were just present as secondary colonizers. the rot fungi penetrated mainly through wounds and bruises on the surface of fruits. mature green fr ...19969208478
contact-sensing by hyphae of dermatophytic and saprophytic fungi.contact-sensing or thigmotropism is the directional growth response of cells in relation to topographical guidance cues. thigmotropism is thought to play a major role in the location of infectable sites on plants by phytopathogenic fungi and has recently been shown to be a property of hyphae in the human pathogenic fungus candida albicans. here we show that hyphae of the dermatophytes epidermophyton floccosum, microsporum canis and trichophyton mentagrophytes reorientate their direction of growt ...19979292428
4-dihydromethyltrisporate dehydrogenase from mucor mucedo, an enzyme of the sexual hormone pathway: purification, and cloning of the corresponding gene.we have purified the nadp-dependent 4-dihydromethyltrisporate dehydrogenase from the zygomycete mucor mucedo. the enzyme is involved in the biosynthesis of trisporic acid, the sexual hormone of zygomycetes, which induces the first steps of zygophore development. protein was obtained from the (-) mating type of m. mucedo after induction with trisporic acid, and purified by gel filtration and affinity chromatography steps. on sds-page a band with an apparent molecular mass of 33 kda was ascribed t ...19968828234
sequence comparison of a segment of the gene for 3-hydroxy-3-methylglutaryl-coenzyme a reductase in zygomycetes.in this paper we compare the sequences of a segment of the 3-hydroxy-3-methylglutaryl-coenzyme a (hmg-coa) reductase gene, isolated from eleven different strains belonging to four species of the fungal order mucorales, parasitella parasitica, absidia glauca, mucor mucedo (mucoraceae) and blakeslea trispora (choanephoraceae). the segment was obtained by polynucleotide-chain-reaction amplification with primers derived from conservative regions of the gene. for the species m. mucedo and p. parasiti ...19948125097
responsiveness of basophil granulocytes of horses suffering from chronic obstructive pulmonary disease to various allergens.as basophils are the major effector cells of allergic reactions, confirmation of the allergic etiology of chronic obstructive pulmonary disease (copd) was sought by the demonstration of a specific in vitro response of equine basophilic blood cells to some potential allergens (aspergillus, cladosporidium, mucor, penicillium, extracts of dust particles of hay and straw). the allergen induced degranulation of basophils and the histamine and protease release from basophils during incubation with the ...19937507274
toxigenicity of fungi from grain sorghum.the mycoflora of nine varieties of grain sorghum was determined by plating serial dilutions of ground samples on rose bengal-streptomycin agar. seventeen species of fungi representing 10 genera were identified. curvularia, penicillium, mucor, and aspergillus were dominant genera. extracts of p. herquei were highly toxic to brine shrimp, while those of c. clavata, c. lunata, and mucor mucedo showed low to moderate toxicity. extracts of c. clavata, c. lunata, and m. mucedo were highly toxic to chi ...19817196500
anomalous cell wall synthesis in mucor mucedo (l.) fres. induced by some fungicides and other compounds related to the problem of dimorphism.an anomalous cell wall thickening in mucor mucedo is induced already within 60-120 min by some fungicides (etridiazol, chloroneb, pentachloronitrobenzene, dicloran, drazoxolon, biphenyl) as well as with a n2-atmosphere or high concentrations of glucose, but not with 2,4-dinitrophenol, chlorinated phenols, dichlofluanid and antimycin a. this effect seems to be identical to the change from the mycelial (m-) to the yeast (y-) form in dimorphic fungi, which can be achieved by culture conditions as w ...19827123995
[effect of pentachloronitrobenzene (pcnb) on the ultrastructure of mucor mucedo and phytophthora cactorum].the effect of pcnb in various concentrations on the ultrastructure of mucor mucedo and phytophthora cactorum was analyzed after an incubation period of 2 hours. the most striking effect in both fungi was a diffuse lysis of the internal structure of the mitochondria which differs markedly from the lysis induced by etridiazol (terrazol). moreover an enlargement of the perinuclear space and an increased formation of vacuoles was observed. in mucor mucedo, but not in phytophthora cactorum a patholog ...19827123992
[production of milk-clotting enzymes by aerated submerged culture of a strain of mucor mucedo]. 19846393666
antifungal properties of 3-n-alkyn-1-ols and synergism with 2-n-alkyn-1-ols and ketoconazole.twelve 3-n-alkyn-1-ols (c4-c12, c14, c16, and c18) were tested against aspergillus oryzae, aspergillus niger, trichoderma viride, and myrothecium verrucaria in sabouraud dextrose agar at ph 5.6 and 7.0. toxicity to candida albicans, candida tropicalis, trichophyton mentagrophytes, and mucor mucedo was determined in the same medium at ph 5.6 and 7.0 in the absence and presence of 10% beef serum. fungitoxicity was strongly influenced by chain length, slightly by ph of the medium, and significantly ...19854020633
mycoflora and vitamin content of sun-dried food condiments in nigeria.the fungi most frequently isolated (of 20-100% incidence) from fresh okra, pepper fruits and melon seeds are botryodiplodia theobromae, rhizopus stolonifer, trichoderma harzianum, mucor mucedo and fusarium oxysporum. the major mycoflora of the fruits which had been sun-dried for twenty days are comprised of aspergillus spp and penicillium spp which initially had formed the minor components (of less than 20% incidence) of the colonising population. the effect of sun-drying on the fruits was a sta ...19873507682
cyclic adenosine 3',5'-monophosphate and germination of sporangiospores from the fungus mucor.cyclic adenosine 3',5'-monophosphate (camp) metabolism was examined in germinating sporangiospores of mucor genevensis and mucor mucedo. exogenous camp prevented normal hyphal development from sporangiospores. internal pools of camp fluctuated profoundly during development. spherical growth of the spores was characterized by large pools of camp whereas germ tube emergence and hyphal elongation were characterized by small pools of camp. these observations suggest a possible role for camp in spora ...19806254459
rhizoferrin: a complexone type siderophore of the mucorales and entomophthorales (zygomycetes).the present investigation presents evidence that rhizoferrin, a novel polycarboxylate or complexone-type siderophore, originally isolated from rhizopus microsporus, represents the common siderophore within the zygomycetes. thus, rhizoferrin could be detected by hplc analysis in various families of the mucorales, e.g., rhizopus microsporus var. rhizopodiformis, mucor mucedo and phycomyces nitens (mucoraceae), chaetostylum fresenii and cokeromyces recurvatus (thamnidiaceae), cunninghamella elegans ...19921387861
antifungal activity of 4-substituted crotonic acid esters.twenty-three 4-substituted crotonic acid esters were tested for antifungal activity against candida albicans, aspergillus niger, mucor mucedo, and trichophyton mentagrophytes. for the analogues of the methyl ester containing substituents in the 4 position, the following order of fungitoxicity was observed: i greater than br greater than cl greater than ch3s greater than ch3o greater than f=h. of the homologues of the esters of the 4-iodo and 4-bromo compounds which included methyl, ethyl, n-prop ...1976787525
the fungal flora of loganberries in relation to storage and spoilage.botrytis cinerea, cladosporium spp., penicillium spp., aureobasidium pullulans, mucor mucedo, phoma state of didymella applanata, cryptococcus laurentii var. laurentii, c. albidus var. albidus, candida sake and sporobolomyces roseus were consistently present on freshly harvested loganberries. botrytis cinerea was the main spoilage organism with mucor mucedo of minor importance. the importance of benomyl-resistant strains of b. cinerea is discussed.1977557301
antifungal properties of 2-bromo-3-fluorosuccinic acid esters and related compounds.twelve esters (c1-c6) of erythro- and threo-2-bromo-3-fluorosuccinic acid and related compounds were tested for antifungal activity against candida albicans, aspergillus niger, mucor mucedo, and trichophyton mentagrophytes at ph 5.7 and 7.0 in the absence and presence of 10% beef serum in sabouraud dextrose agar. at ph 7.0 in the presence of 10% beef serum, no consistent pattern in the fungitoxicity of the erythro- and threo-2-bromo-3-fluorosuccinate esters was seen. increasing the length of the ...1977321785
microbial and chemical transformations of some 12,13-epoxytrichothec-9,10-enes.resting cells of streptomyces griseus, mucor mucedo, and a growing culture of acinetobacter calcoaceticus when mixed with compounds related to 12,13-epoxytrichothec-9-ene-4beta,15-diacetoxy-3alpha-ol(anguidine) produced a series of derivatives that were either partially hydrolyzed or selectively acylated. these derivatives showed marked differences in activities as assayed by antifungal and tissue culture cytotoxicity tests.1978100053
antifungal properties of alpha,omega-alkanedicarboxylic acids and their dimethyl esters.thirteen alpha, omega-alkanedicarboxylic acids (c2-c12, c14, and c16) and their dimethyl esters were tested against aspergillus niger, trichoderma viride, and myrothecium verrucaria in sabourauc dextrose agar at ph 4.0 and 5.6. toxicity to canadida albicans, trichophyton mentagrophytes, and mucor mucedo was determined in the same medium at ph 5.6 and 7.0 in the absence and presence of 10% beef serum. the dicarboxylic acids possessed very poor to no antifungal activity against all six fungi. the ...19769194
mycobiota and mycotoxins in bee pollen collected from different areas of slovakia.contamination by microscopic fungi and mycotoxins in different bee pollen samples, which were stored under three different ways of storing as freezing, drying and uv radiation, was investigated. during spring 2009, 45 samples of bee-collected pollen were gathered from beekeepers who placed their bee colonies on monocultures of sunflower, rape and poppy fields within their flying distance. bee pollen was collected from bees' legs by special devices placed at the entrance to hives. samples were ex ...201121749250
sexual reactions in mortierellales are mediated by the trisporic acid system.several species of mortierella (mortierellales, zygomycota) were examined for substances regulating their sexual reactions. compounds isolated from both mated and single growing mortierella strains were purified by thin layer chromatography. some of these compounds showed uv absorbance-characteristics similar to those of trisporoids, a group of compounds involved in sexual regulation in mucorales. a compound with a 4-dihydromethyltrisporate-like absorbance spectrum was detected. to test for the ...200312951800
4-dihydrotrisporin-dehydrogenase, an enzyme of the sex hormone pathway of mucor mucedo: purification, cloning of the corresponding gene, and developmental expression.the nadp-dependent 4-dihydrotrisporin-dehydrogenase is a (-) mating-type-specific enzyme in the pathway from beta-carotene to trisporic acid. this substance and its isomers and derivatives represent the general system of sexual communication in zygomycetes. the (-) mating type of mucor mucedo was stimulated by trisporic acid and the enzyme was purified by ion exchange and affinity chromatography. several peptides of the 26-kda protein, digested with trypsin, were sequenced by mass spectrometry. ...200918931040
[concentration of allergic fungi spores in the air of flats in lódź].the real contribution of moulds to the pathogenesis of allergic diseases remains unknown, although positive skin prick tests and/or specific serum ige to moki allergens can be detected in 1-5% of atopic patients. a significant problem in assesment of exposure to mould allergens, resulting with difficulty in standarization of methods. the aim of this work was to assess the concentration of spores of 8 mould species in flats inhabited by peoples who bont show any symptoms of allergy. the open petr ...199916886472
[the effect of cytochalasin a on the composition of subcellular fractions of hyphae in the growth of mucor mucedo l. i. composition of the plasmalemma].the plasma membrane of young hyphae of mucor mucedo l. growing in presence or absence of cytochalasine a was isolated by continuous density gradient centrifugation using percoll at 10% or on discontinuous sucrose density gradient. isolated membranes were characterized by enzymatic markers and cytochemical reactions, using electron microscopy. lipid composition and protein content were determined. from the enzymatic point of view, the cytochalasine a induced a decrease (60%) in atpase activity an ...19882974755
isolation and characterization of an endo-beta-d-glucuronidase from the fungus kobayasia nipponica.an endo-beta-d-glucuronidase was isolated and characterized from kobayasia nipponica. the enzyme was purified by ammonium sulfate fractionation, cm-sephadex chromatography, gel filtration with sephacryl s-200, and heparin-sepharose chromatography. the enzyme shows the following properties: optimum ph 5.0, thermal stability below 37 degrees c, ph stability 5-6, optimum temperature 45-55 degrees c, and km 0.12% for l-idurono-d-glucuronan (protuberic acid (pa), l-idua:d-glcua = 1:2) from kobayasia ...19846530398
methyl trisporate e. a sex pheromone in phycomyces blakesleeanus.combined mating type cultures of phycomyces blakesleeanus accumulate 41 mg of trisporic acids/l of medium, of which 30% is trisporic acid e. the methyl ester of trisporic acid e exhibits the same zygophore -inducing activity in bioassays with p. blakesleeanus and mucor mucedo as does the pheromone methyl trisporate c. the structure of methyl trisporate e is 1,5-dimethyl-2-hydroxyl-4-oxo-6-(2'-hydroxyl-6'- methylocta -5',7'-d ien-8'-yl) -5-cyclohexene-1-carboxylic acid methyl ester.19846547137
[biosynthesis of linoleic acid by the filamentous mould: mucor mucedo (siphomycetes) (author's transl)]. 1979496223
purification and characterization of a glutathione s-transferase from mucor mucedo.an intracellular glutathione transferase was purified to homogenity from the fungus, mucor mucedo, using deae-cellulose ion-exchange and glutathione affinity chromatography. gel filtration chromatography and sds-page revealed that the purified gst is a homodimer with approximate native and subunit molecular mass of 53 kda and 23.4 kda, respectively. the enzyme has a pi value of 4.8, a ph optimum at ph 8.0 and apparent activation energy (ea) of 1.42 kcal mol(-1). the purified gst acts readily on ...200516209109
effects of different fungal elicitors on growth, total carotenoids and astaxanthin formation by xanthophyllomyces dendrorhous.six fungal elicitors prepared from rhodotorula rubra, rhodotorula glutinis, panus conchatus, coriolus versicolor, mucor mucedo, mortieralla alpina m-23 were examined to determine their effects on the growth, total carotenoids and astaxanthin formation by xanthophyllomyces dendrorhous. the results showed that different fungal elicitor could cause diversely stimulating effects. among the fungal elicitors tested, the m. mucedo elicitor concentration of 30 mg l(-1) promoted the biomass and total car ...200616154499
[misting-fountain-alveolitis].a 22-year-old woman developed recurrent episodes of fever, cough and dyspnea after repeated exposure to a misting fountain at home. a diagnosis of extrinsic allergic alveolitis (eaa) was made by detection of serum antibodies against the fountain water, by culture of bacillus subtilis, mucor racemosus, mucor mucedo, and saccharomyces cerevisiae from the water, and by detection of specific igg antibodies against bacillus subtilis and the mucores. the diagnosis was confirmed by a restrictive lung f ...200415343489
identification of fungal diseases associated with imported wheat in iranian silos.in this study, imported wheat varieties used for cookies and bread making were evaluated for the presence of fungal diseases in the silos. grain samples were taken and cultured on nutrient agar medium and sterile papers impregnated with nutrient. the results showed the presence of pathogenic fungi such as ulocladium sp., cladosporium sp., alternaria sp., rhizopus nigricans, penicillium sp. and trichothecium sp. in varieties from australian, mucor sp., r. nigricans, fusarium sp., a. triticum, hel ...200315151286
decolourisation of textile and dye amended soils by fungi.four fungi viz. aspergillus niger, curvularia lunata, fusarium oxysporum, mucor mucedo isolated from textile and dye contaminated soils were tried for their efficiency in colour removal. the physical, chemical and biological characteristics were analysed and related to the process of decolourisation. the role of extracellular enzymes, alpha-amylase, protease, catalase and glucose oxidase was studied during 7, 14, and 21 days of incubation period. a. niger and m. mucedo were resistant in the soil ...200212968727
microbial transformations of the antimelanoma agent betulinic acid.microbial transformation studies of the antimelanoma agent betulinic acid (1) were conducted. screening experiments showed a number of microorganisms capable of biotransforming 1. three of these cultures, bacillus megaterium atcc 14581, cunninghamella elegans atcc 9244, and mucor mucedo ui-4605, were selected for preparative scale transformation. bioconversion of 1 with resting-cell suspensions of phenobarbital-induced b. megaterium atcc 14581 resulted in the production of the known betulonic ac ...200011141108
studies on fungal polysaccharides. x. extracellular heteroglycans of absidia cylindrospora and mucor mucedo. 19725032471
[infection of the postoperative cavity of the ear with the mucor mucedo fungus]. 20046003064
utilization of fatty acids by and lipolytic activities of mucor mucedo. 195413183893
microbial transformations of isocupressic acid.microbial transformations of the labdane-diterpene isocupressic acid (1) with different microorganisms yielded several oxygenated metabolites that were isolated and characterized by ms and nmr spectroscopic analyses. nocardia aurantia (atcc 12674) catalyzed the cleavage of the 13,14-double bond to yield a new nor-labdane metabolite, 2. cunninghamella elegans (-) (nrrl 1393) gave 7beta-hydroxyisocupressic acid (3) and labda-7,13(e)-diene-6beta,15, 17-triol-19-oic acid (4), and mucor mucedo (atcc ...19989677275
morphological changes of the filamentous fungus mucor mucedo and inhibition of chitin synthase activity induced by anethole.trans-anethole (anethole), a major component of anise oil, has a broad antimicrobial spectrum with antimicrobial activity relatively weaker than those of well-known antibiotics, and significantly enhances the antifungal activity of polygodial and dodecanol against the baker's yeast saccharomyces cerevisiae and human pathogenic yeast candida albicans. however, the antifungal mechanism of anethole is unresolved. anethole demonstrated antifungal activity against the filamentous fungus, mucor mucedo ...201121721062
the mating-related loci sexm and sexp of the zygomycetous fungus mucor mucedo and their transcriptional regulation by trisporoid pheromones.the putative mating type locus of mucoralean fungi consists of a single hmg-domain transcription factor gene, sexm or sexp, flanked by genes for an rna helicase and a tp transporter. we used degenerated primers derived from the amino acid sequence of the rna helicase for sequencing a fragment of this gene from mucor mucedo. by inverse pcr this fragment was extended to obtain partial sequences of the sex loci from both mating types of mucor mucedo. the sex loci in mucor mucedo reflect the general ...201222262094
early and late trisporoids differentially regulate β-carotene production and gene transcript levels in the mucoralean fungi blakeslea trispora and mucor mucedo.the multistep cleavage of carotenoids in mucorales during the sexual phase results in a cocktail of trisporic acid (c18) sex pheromones. we hypothesized that the c18 trisporoid intermediates have a specific regulatory function for sex pheromone production and carotenogenesis that varies with genus/species and vegetative and sexual phases of their life cycles. real-time quantitative pcr kinetics determined for blakeslea trispora displayed a very high transcript turnover in the gene for carotenoid ...201324056470
chemical composition, antioxidant and antimicrobial activities of basil (ocimum basilicum) essential oils depends on seasonal variations.chemical composition, antioxidant and antimicrobial activities of the essential oils from aerial parts of basil (ocimum basilicum l.) as affected by four seasonal, namely summer, autumn, winter and spring growing variation were investigated. the hydro-distilled essential oils content ranged from 0.5% to 0.8%, the maximum amounts were observed in winter while minimum in summer. the essential oils consisted of linalool as the most abundant component (56.7-60.6%), followed by epi-α-cadinol (8.6-11. ...200826065762
effects of 7-hydroxycalamenene isolated from croton cajucara essential oil on growth, lipid content and ultrastructural aspects of rhizopus oryzae.the leaves and bark of croton cajucara, a shrub from the amazon region, have been used in folk medicine to treat diabetes, malaria, and gastrointestinal and liver disorders. the essential oil from the leaves, rich in linalool, presented antileishmanial and antimicrobial activities. a chemotype of this species was found with an essential oil rich in 7-hydroxycalamenene. during our studies of the c. cajucara essential oil, we isolated 7-hydroxycalamenene at > 98 % purity. the minimum inhibitory co ...201424841967
[risk factors of invasive fungal infections in patients admitted to non- hematological oncology department and pediatric intensive care unit].to determine risk factors of invasive fungal infections (ifi) in patients admitted to non-hematological oncology department and pediatric intensive care unit (picu), in order to improve diagnostic level of invasive fungal infections.201324225291
post-translational regulation by structural changes of 4-dihydromethyltrisporate dehydrogenase, a key enzyme in sexual and parasitic communication mediated by the trisporic acid pheromone system, of the fungal fusion parasite parasitella parasitica.sexual communication between complementary mating partners in the fungal group of zygomycetes is mediated by the trisporoid pheromone system. a key enzyme towards biosynthesis of hormonally active trisporoids is 4-dihydromethyltrisporate dehydrogenase (tsp1), an enzyme occurring in all zygomycetous fungi. trisporic acid and some of its precursor molecules serve as pheromones for recognizing complementary mating partners and for induction of the differentiation program towards sexual spore format ...201727864097
composition and morphology characterization of exopolymeric substances produced by the pah-degrading fungus of mucor mucedo.to explore the role of exopolymeric substances (eps) in the process of polycyclic aromatic hydrocarbons (pah) biodegradation, the characteristics of eps isolated from a pah-degrading fungus were investigated firstly by spectrometric determination, microscopic observation, fourier transform-infrared spectroscopy (ft-ir), and three-dimensional excitation-emission matrix fluorescence spectroscopy (3d-eem), and then the pah-degrading ability of isolated eps was evaluated. the eps compositions and mo ...201626782320
lysine and glutamic acids as the end products of multi-response of optimized fermented medium by mucor mucedo kp736529.amino acids are important for living organisms, they acting as crucial for metabolic activities and energy generation, wherein the deficiency in these amino acids cause various physiological defects.201729023029
correlation between sequence, structure and function for trisporoid processing proteins in the model zygomycete mucor mucedo.terpenoids, steroids, carotenoids, phytoenes and other chemically related substance groups fulfill multiple functions in all realms of the organismic world. this analysis focuses on trisporoids that operate as pheromones in the phylogenetically ancient fungal group of mucoralean zygomycetes. trisporoids serve as pheromones for recognizing complementary mating partners and for inducing the differentiation program towards sexual spore formation. trisporoids are synthesized by oxidative degradation ...201323261395
a thermolabile aspartic proteinase from mucor mucedo dsm 809: gene identification, cloning, and functional expression in pichia pastoris.in this study, the cdna encoding the aspartic proteinase of mucor mucedo dsm 809 has been identified by rna ligased-mediated and oligo-capping rapid amplification of cdna ends (race) technique. the gene contained an open reading frame of 1,200 bp and encoded for a signal peptide of 21 amino acid residues. two n-glycosylation sites were observed within the identified sequence. the proteinase gene was cloned into the vector pgapzαa and expressed in pichia pastoris x-33 for the first time. the prot ...201323065363
purification and characterization of a novel phospholipid transfer protein from filamentous fungi.1. we have isolated from mycelia of mucor mucedo, a filamentous fungus, a phospholipid transfer protein. 2. the purification steps were gel filtration, hydroxyapatite chromatography, blue affinity column and fast protein liquid chromatography on anion exchanger. 3. a purified protein was obtained with a molecular mass of 24 kda and a pi of 5.05 and its n-terminal sequence was established. 4. this protein transfers phosphatidylinositol, as well as phosphatidylcholine and phosphatidylethanolamine.19902328823
[the effect of cytochalasin a on the composition of subcellular fractions of hyphae in the growth of mucor mucedo. ii. composition of the cell wall].walls of young hyphae of mucor mucedo l. growing in the presence or absence of cytochalasin a were isolated and their chemical content determined. cytochalasin a induced modified proportions of various monomers resulting in a reduction of the (neutral sugars + glucuronic acid)/glucosamine ratio. the walls contained less proteins but more chitin-chitosan and phosphate. these modifications are discussed in relation to ultrastructural changes described previously.19883208202
morphogenesis in mucor mucedo: mutations affecting gamone response and organ differentiation.mutants of mucor mucedo minus strain that are affected in their trisporic acid (ta) mediated zygophore formation have been isolated. we have found mutants with cold sensitive (cs), with temperature sensitive (ts) and without zygophore formation as well as mutants with unstable zygophores (zst-). from the appearence of certain pleiotropic phenotypes we deduce a one-dimensional sequence of states of competence of the mycelium to form different organs. ta appears to be a growth substance for zygoph ...1978634277
the hyphal wall of mucor mucedo. 2. hexosamine-containing polymers.nitrous acid, which specifically depolymerises polymers containing hexosamines with a primary amino group, was used to analyse the hexosamine-containing polymers in the hyphal wall of mucor mucedo. n-acetylglucosamine was found to occur in three polymeric fractions. one fraction which was solubilised by hno2 treatment contained-n-acetylglucosamine interspersed with glucosamine; no homopolymer of glucosamine (chitosan) was detected. another fraction became hno2-soluble after treatment with pronas ...1977923598
the hyphal wall of mucor mucedo. 1. polyanionic polymers.treatment of isolated hyphal walls of mucor mucedo with nitrous acid resulted in the release of two water-soluble polyanions: (a) a glycuronan, containing all the neutral sugars and uronic acid present in the hyphal wall and (b) an inorganic polyphosphate. the glycuronan could also be extracted quantitatively with salt solutions of high ionic strength and partially with a solution of potassium hydroxide. this is presented as evidence that the glycuronan is a genuine constituent of the cell wall, ...1977923597
[mode of action of terrazoleon mucor mucedo].it is assumed that the fungistatic effect of terrazol in mucor mucedo is induced by a liberation of phospholipases within within the mitochondria and perhaps at other membranes. in isolated mitochondria a rapid formation of lysolecithin can be demonstrated at low concentrations of terrazol. this would explain the lytic symptoms in mitochondria visible by electron microscopy. lipid peroxidation could not be demonstrated. the only antidots at present known for the growth inhibiting effect of terra ...1977868082
[effect of terrazol on the ultrastructure of mucor mucedo].terrazol, a systemic fungicide showing high specifity to oomycetes, inhibits the apical growth of hyphae and promotes at lower concentrations the thickening of the cell wall in mucor mucedo. as revealed by ultrastructural analysis, particularly the fine structure of some membrane systems is influenced. in the first place the inner membrane of the mitochondria is attacked leading to a complete lysis of mitochondria. however, the sensitivities within a given population are different. the plasmalem ...19751210350
sex specificity of hormone synthesis in mucor mucedo.sex specificity is observed in mating types of the fungus oucor mucedo with respect to the production of 4-hydroxy methltrisporates (plus mating type) and trisporins (minus mating type), and in the conversion of these metabolites to trisporic acids by the mating partner. these compounds induce zygophores on the opposite mating type only.19751115561
[use of cytochemical methods for diagnosis in fungicide research].cytochemical localization of acid and alkaline phosphatases, cytochrom c-oxidase, peroxidases and catalase was carried out on phytophthora infestans (mont.) de by. and mucor mucedo (l.) fres. first results are obtained about the influence of sublethal dosages of fungicidal compounds on the demonstration of these enzymes in the electron-microscope.19846326192
hormonal interactions in mucor mucedo and blakeslea trispora.evidence is presented that progametangia in both the plus and the minus mating types of mucor mucedo can be induced by one substance, namely (-)-trisporic acid b. a method is described for the determination of the concentration of the sex factors (trisporone, trisporic acid b, trisporic acid c) in mated cultures of mucorales by polarography. it can be demonstrated that the amount of plus mycelium is limiting for the production of the sex factors in blakeslea trispora. it is shown that the minus ...19705413820
4-dihydromethyltrisporate dehydrogenase, an enzyme of the sex hormone pathway in mucor mucedo, is constitutively transcribed but its activity is differently regulated in (+) and (-) mating types.4-dihydromethyltrisporate dehydrogenase (tdh) converts the (+) mating type sex pheromone 4-dihydromethyltrisporate into methyltrisporate. in mucor mucedo, this conversion is required only in the (-) mating type. expression of the tdh encoding tsp1 gene was analyzed qualitatively using reverse-transcribed pcr. tsp1 is constitutively transcribed in the (+) and in the (-) mating type, irrespective of the mating situation. by immunodetection, the translation product is also formed constitutively. in ...200516043374
biological activity of trisporoids and trisporoid analogues in mucor mucedo (-).in the course of their sexual interactions, zygomycete fungi communicate via an elaborate series of carotene-derived compounds, namely trisporic acid and its biosynthetic progenitors. a novel building-block strategy allowed the systematic generation of structurally modified trisporoids along with putative early biosynthetic precursors for physiological tests. the impact of discrete structural elements was documented by the ability of individual compounds to induce sexually committed hyphae in mu ...200515913673
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