| [allergy to house dust in patients with asthma. iii. ige level and specific ige antibodies against house dust allergens, dermatophagoides pteronyssinus, aspergillus fumigatus and cladosporium herbarum in the serum of patients with asthma]. | | 1977 | 319433 |
| detection of mutagens produced by fungi with the salmonella typhimurium assay. | forty-one fungal isolates (one isolate per species) representing common plant pathogens and food crop contaminants were grown on sterile, polished rice and assayed for mutagenic activity in the salmonella typhimurium-microsome system. initially, single doses of aqueous and chloroform extracts of the moldy rice were assayed against the ta100 tester strain by incorporating extracts into the growth medium and by applying small quantities on disks placed on the agar surface. suspected activity was e ... | 1978 | 354528 |
| cladosporium herbarum extract characterized by means of quantitative immunoelectrophoretic methods with special attention to immediate type allergy. | freeze-dried extract of cladosporium herbarum link ex fr. was obtained by growing, harvesting, extracting, centrifuging, dialysing and freeze-drying. quantitative immunoelectrophoresis using rabbit antibodies revealed the extraction procedure to be reproducible and the extract to be composed of 57 antigens, none of which originated from the substrate used in the growth. the molecular weight distribution and the approximate molecular weight of some antigens of c. herbarum were obtained using gel ... | 1977 | 563379 |
| crossed radioimmunoelectrophoretic studies of distinct allergens in two extracts of cladosporium herbarum. | cladosporium herbarum was supplied from two sources and extracted identically. antisera against the extracts were produced in rabbits and two reference patterns were established using crossed immunoelectrophoresis (cie). both patterns showed more than 60 precipitates but less than 50% of the detectable antigens appeared to be identical in the two extracts. the allergens were identified by means of crossed radioimmunoelectrophoresis (crie) using sera from 35 individuals with proven or suspected a ... | 1979 | 571415 |
| fungal antigens as a source of sensitization and respiratory disease in scottish maltworkers. | mycological and serological studies were carried out as part of a survey of respiratory disease in scottish maltworkers. 70% of stained sputum smears from 574 workers showed the presence of higher plant cells and/or myclelia, and the spores of common environmental fungi. penicillium spp. (90%), rhizopus stolonifer (48%) and yeasts (53%) were the dominant fungi in 699 sputum cultures, and showed a similar proportional distribution in 327 samples of grain, malt, culms and dusts from fifty-six malt ... | 1977 | 589785 |
| studies on mycoflora of salt marshes in egypt. iv. osmophilic fungi. | ninety-five species and seven varieties which belong to thirty-nine genera were isolated from 74 soil samples from salt marshes. at 28 degrees c, on 30% sucrose czapek's agar, ninety species and four varieties which belong to thirty-six genera were encountered, from which a. niger, a. fumigatus a. terreus and p. notatum were the most frequent. on 60% sucrose czapek's agar, sixty-three species and three varieties were recovered which belong to twenty-five genera, from which a. niger, a. fumigatus ... | 1977 | 600306 |
| cellulose-decomposing fungi of salt marshes in egypt. | seventy-five species and three varieties which belong to thirty-four genera were identified from 74 soil samples collected from salt marshes in egypt. the most frequent fungi were aspergillus fumigatus, aspergillus niger, cladosporium herbarum and alternatia alternata, followed by aspergillus terreus, curvularia spicifera and penicillium notatum. six genera were of moderate occurrence: penicillium, fusarium, curvularia, rhizopus, stachybotrys, and chaetomium. five genera were of low occurrence: ... | 1978 | 624509 |
| a study on fungal radioresistance and sensitivity. | lower doses of gamma and x-rays (5 and 1 krad, respectively) stimulated the spore germination and growth of 7 tested fungi. higher doses (500 and 100 krad, respectively) revealed that epicoccum nigrum was the most radioresistant, while alternaria humicola, stemphylium verruculosum, rhizopus nigricans, and cladosporium herbarum were of moderate resistance. aspergillus niger and penicillium oxalicum were the most radiosensitive. protein and polysaccharide synthesis were inhibited in the mycelium d ... | 1977 | 857505 |
| high-pressure liquid-chromatographic assay of nucleotide-pool concentrations during polysaccharide biosynthesis in four ascomycetes. | high-resolution liquid-chromatographic methods developed for analyzing nucleotide pools at the nanogram level in four representative species of ascomycetes (penicillium citrinum, aspergillus niger, fusarium moniliforme, and cladosporium herbarum) were used to study polysaccharide biosynthesis. nucleotides extracted from the mycelial mat were preseparated from interfering polysaccharides, glycoproteins, and nucleic acids on a column of biogel p-2. resolution of 18 nucleotides from each fungal spe ... | 1977 | 912696 |
| possible role of solar radiation on the viability of some air fungi in egypt. | the high occurrence of alternaria humicola and the moderate occurrence of stemphylium verruculosum, rhizopus nigricans, and epicoccum nigrum in the air of egypt could be correlated to their apparent resistance to solar radiation. cladosporium herbarum, aspergillus niger, and penicillium oxalicum, although highly occurring, were light-sensitive. the miniumum solar radiation in november, coupled with the highest total fungal catch, dominated by cladosporium herbarum, reveals the importance of sola ... | 1976 | 1037050 |
| pityrosporum ovale and atopic dermatitis in children and young adults. | children aged 0-21 years, 60 children with atopic dermatitis (ad), 40 children with rhinoconjunctivitis and or asthma (ra) and 40 children with no atopic history (hc) were studied to evaluate the relationship between skin colonisation with pityrosporum ovale and the occurrence of specific ige antibodies to p. ovale. the following studies were done: culture for p. ovale, measurement of ige antibodies to p. ovale (skin prick test, rast), candida albicans, and cladosporium herbarum (rast) and igg a ... | 1992 | 1357856 |
| ige antibodies to pityrosporum orbiculare and staphylococcus aureus in patients with very high serum total ige. | serum ige antibodies were detected with the radioallergosorbent test (rast) to a panel of allergens which included pityrosporum orbiculare, candida albicans, trichophyton rubrum, cladosporium herbarum, staphylococcus aureus, animal dander, deciduous tree pollens, grass pollens and foods in 81 consecutive patients with total ige greater than or equal to 3000 ku/l. data on atopic and infectious disease characteristics were collected by a questionnaire. ige antibody concentrations to p. orbiculare ... | 1992 | 1525694 |
| keratinophilic fungi and other moulds associated with air-dust particles from egypt. | one-hundred and eleven species and three species varieties belonging to 39 genera were collected from 50 dust samples on the five media used at 28 degrees c. using the hair-baiting technique with horse hair, 10 species of chrysosporium were isolated: c. asperatum, c. state of arthroderma tuberculatum, c. indicum, c. inops, c. keratinophilum, c. merdarium, c. pannorum, c. queenslandicum, c. tropicum and c. xerophilum. true dermatophytes were isolated: trichophyton verrucosum and trichophyton sp. ... | 1990 | 1702081 |
| aeroallergen analyses and their clinical relevance. i. immunochemical quantification of allergens by rast-inhibition, mab-elisa, basophil histamine release, and counter current immuno electrophoresis. | the aim was to compare ige and igg4 rast-inhibition assay (ri), monoclonal antibody elisa (mab-elisa), counter current immuno electrophoresis (ccie) and histamine release from basophil leukocytes (hr) for allergen quantification with special reference to aeroallergen detection. as components of indoor aeroallergens, cat, dog, and derm. pter. allergen extracts were selected for the experiments. to evaluate unspecific interference, these allergens were compared mutually and with cladosporium herba ... | 1991 | 1724591 |
| further structural studies of the carbohydrate moiety of the allergen ag-54 (cla h ii) from the mould cladosporium herbarum. | the carbohydrate moiety of the glycoprotein allergen ag-54, isolated from the mould cladosporium herbarum, has been characterised partly, using acetolysis, methylation analysis, and n.m.r. spectroscopy. ag-54 contained a highly branched galactoglucomannan and two branched mannogluco-oligosaccharide chains. the oligosaccharides contained terminal, (1----4)-, and (1----4,6)-linked alpha-glc residues and terminal, (1----2)-, and some (1----3)-linked alpha-man residues. the n.m.r. data indicated the ... | 1991 | 1769019 |
| clustering of fungal allergen-specific ige antibody responses in allergic subjects. | statistical analysis of specific ige immune responses to a panel of nine allergens from 2,094 patients demonstrated significant clustering between aspergillus fumigatus, alternaria alternata, and cladosporium herbarum, not explained by crossreactive elements. this may represent a linked, high responder status to these fungal antigens in the allergic population. | 1991 | 1994786 |
| enzyme-linked immunosorbent assay for detection of molds in cheese and yogurt. | an enzyme-linked immunosorbent assay was developed for the detection of molds in dairy products. new zealand white female rabbits were immunized with .45 mg of partially purified extracellular antigen from freeze-dried culture filtrates of aspergillus versicolor, cladosporium herbarum, geotrichum candidum, mucor circinelloides, and penicillium chrysogenum. blood was drawn at various intervals, and antibodies were separated and purified. antibody-peroxidase conjugates were prepared with the follo ... | 1990 | 2099362 |
| houses and allergic respiratory syndromes. | a study was carried out in 49 flats in bucharest inhabited by 265 persons. a quantitative analysis of fungi spores was performed as well as allergologic tests with penicillium and aspergillus fungi (a13), aspergillus niger (m2), alternaria tenuis (m1), cladosporium herbarum (m3), dermatophagoides pteronissinus (allergenic products supplied by the bencard firm). the infested flats presented a high percentage of aspergillus niger spores, particularly pathogenic. the main triggering factors for the ... | 1990 | 2100879 |
| [a toxicological evaluation of micromycetes isolated from salmon roe]. | three species of micromycetes (aspergillus clavatus desm., cladosporium herbarum (pers) lk., penicillium canescens sopp.) isolated from the cultivated salmon spawn have been studied for their toxigenic properties and pathogenicity for warm-blooded animals. ld50 are determined for mice perorally administered mycelium suspension of the first two species; the third of the studied species proved to be nontoxicogenic. the possible pathogenic action of mycotoxins on fish spawn in aquarium is discussed ... | 1990 | 2198440 |
| fungi associated with dried fish in sri lanka. | the mycoflora of dried-salted fish from markets in kandy, sri lanka was studied with emphasis on visibly spoiled dried fish. a total of 61 fungal isolates from 25 dried-fish were isolated and identified. the most prevalent fungus was aspergillus niger. species of aspergillus flavus, a. fumigatus, a. glaucus, a. restrictus, aureobasidium spp. basipetospora halophila (a genuinely halophilic fungus) cladosporium herbarum, gliomastix, spp., penicillium chalybeum and penicillium expansum were present ... | 1990 | 2233981 |
| improved in vitro diagnosis of allergy to alternaria tenius and cladosporium herbarum. | to improve the in vitro diagnosis of mould allergy 22 children suffering from allergic asthma caused by alternaria tenius and/or cladosporium herbarum as proven by bronchial provocation test were investigated. partially purified, standardized mould preparations were used in radioallergosorbent test (rast) with conventional and new update mould discs, mould-induced histamine release and immunoblotting. updated rast discs were found to be superior to the old-type discs for the detection of alterna ... | 1987 | 2444126 |
| maxisorp rast. a sensitive method for detection of antigen-specific human ige in culture fluids. | for determination of allergen-specific ige in cell culture supernatants and other highly diluted ige preparations a radioallergosorbent test (rast) based on high adsorption polystyrene test tubes has been developed ("maxisorp rast"). cladosporium herbarum extract was used as a model allergen but timothy grass pollen, house dust mite and dog dander showed similar results. the test showed specificities of both allergen and immunoglobulin isotype and significant correlations (r = 0.67-0.88) with es ... | 1989 | 2469345 |
| dust from carpeted and smooth floors. ii. antigenic and allergenic content of dust vacuumed from carpeted and smooth floors in schools under routine cleaning schedules. | dust samples were collected twice from smooth and carpeted floors in 10 norwegian schools. the content of antigens and allergens of alder (alnus incana), birch (betula verrucosa), timothy (phleum pratense), cat and dog dander, house dust mite (dermatophagoides farinae), mould (cladosporium herbarum), hen egg white and codfish (diii) were investigated by crossed immunoelectrophoresis (cie), crossed radio immunoelectrophoresis (crie), radio allergosorbent test (rast) inhibition and quantitative pr ... | 1989 | 2478043 |
| studies of atopic patch tests. | 35 patients were patch-tested for 72 h with house dust mite, timothy- and birch-pollen, cladosporium herbarum and hen's egg white allergens, prepared in a cream in concentrations up to 1,000 times the concentration used for skin prick testing. 6 patients developed a total of 10 positive reactions. all the strong positive patch-test reactions occurred in patients with a strong positive skin prick test to the same allergen. immunohistochemical studies of biopsied positive patch-test reactions demo ... | 1989 | 2529724 |
| immunological studies of the glycoprotein allergen ag-54 (cla h ii) in cladosporium herbarum with special attention to the carbohydrate and protein moieties. | the glycoprotein allergen ag-54 (cla h ii) isolated from cladosporium herbarum was split into its carbohydrate and protein moieties by using alkaline-borohydride treatment and deglycosylation, respectively. the native ag-54, the deglycosylated protein and the protein-free carbohydrate moieties were tested for ige-binding activity by radioallergosorbent test inhibition using selected sera from individuals with c. herbarum allergy. the deglycosylated material had a stronger allergenic activity tha ... | 1989 | 2583852 |
| dust from carpeted and smooth floors. i. comparative measurements of antigenic and allergenic proteins in dust vacuumed from carpeted and non-carpeted classrooms in norwegian schools. | dust samples from fitted-carpets and linoleum floors in 12 schools in norway were collected by vacuum cleaning. the presence of antigens and allergens of alder (alnus incana), birch (betula verrucosa), timothy (phleum pratense), mould (cladosporium herbarum), house dust mite (dermatophagoides farinae), cat and dog dander, codfish, hen egg white and human dander were investigated by crossed immunoelectrophoresis (cie), crossed radio-immunoelectrophoresis (crie) and radio-allergosorbent test (rast ... | 1989 | 2752322 |
| mycoflora and mycotoxin-producing fungi of air-dust particles from egypt. | using the dilution-plate method, 27 genera and 64 species were collected from 20 air-dust samples on glucose - (24 genera and 57 species) and cellulose - (21 genera and 45 species) czapek's agar at 28 degrees c. there are basic similarities between the mycoflora of air-dust on the two media and the most prevalent species were aspergillus niger, a. flavus, a. ochraceus, a. terreus, a. versicolor, penicillium chrysogenum, p. funiculosum, alternaria alternata, cladosporium herbarum, fusarium oxyspo ... | 1986 | 2938009 |
| viruses and airborne allergens as precipitants of obstructive respiratory difficulties in children. | findings of virus infection were prospectively studied in 133 episodes of obstructive respiratory difficulty in 90 children aged 2 to 15 years. a viral infection was confirmed in 16 episodes (12%). in a further 11% of cases serological evidence of a recent viral infection was found. respiratory syncytial virus (rsv) accounted for half of the positive viral results. skin prick tests were performed after the second episode of bronchial obstruction. of the 79 children tested, 57% had at least one p ... | 1988 | 3218914 |
| results of biological standardization with standardized allergen preparations. | the aim of biological standardization (bs) is to equilibrate the activity (potency) of allergen extracts from different source materials. this was done by performing skin prick tests (spt) on patients who were sensitive to one of the following 10 allergens: birch, alder, hazel, timothy, rye grass, velvet grass, cultivated rye, mugwort, d. farinae and cladosporium herbarum. patient sensitivity varied within a range of three to four powers of ten for each allergen investigated. the weal size in ea ... | 1987 | 3296840 |
| a double-blind, multicenter immunotherapy trial in children, using a purified and standardized cladosporium herbarum preparation. i. clinical results. | a double-blind histamine placebo controlled immunotherapy trial was performed to investigate the clinical effect of a purified and standardized cladosporium herbarum allergen preparation. thirty children with a clinical history suggesting mould-induced asthma and/or rhinoconjunctivitis were included. the diagnosis was confirmed by positive skin prick test and phadebas rast as well as positive bronchial and/or conjunctival provocation test to cladosporium herbarum. immunotherapy was given for 10 ... | 1986 | 3518526 |
| cross-reactivity between antigens of fungal extracts studied by rast inhibition and immunoblot technique. | we have used the rast-inhibition technique in homologous and heterologous inhibitions of the aspergillus fumigatus, alternaria tenuis, and cladosporium herbarum rast assays followed by the immunoblot technique to assess the degree of shared allergenic determinants in these extracts. in the aspergillus rast, there was little or no inhibition with cladosporium and alternaria, but considerable cross-reactivity was found between alternaria and cladosporium. inhibition by alternaria of the cladospori ... | 1987 | 3549841 |
| basidiomycete mycelia and spore-allergen extracts: skin test reactivity in adults with symptoms of respiratory allergy. | one hundred fifty adults, with respiratory-allergic disease, and 14 control subjects, without symptoms of respiratory allergy, were skin prick tested with 16 common inhalant allergens, 12 extracts of mycelia from basidiomycetes grown in vitro, and/or 10 to 15 basidiospore extracts. eighty-three subjects (58%) had positive skin tests to two or more of the common inhalant allergens. twenty-seven percent of the study subjects had positive skin reactions to one or more of the basidiomycete mycelia e ... | 1986 | 3760405 |
| [frequency of respiratory allergens involved in rhinitis and bronchial asthma in adults. prospective study]. | in a prospective study of 100 adults, we determined the frequency of hypersensitivity to allergens most often diagnosed in patients suffering from rhinitis and bronchial asthma. skin-prick tests and rast for 15 allergens were performed in all patients. the most frequent allergen detected by skin-prick test was the extract "total house dust" (50%) but the corresponding rast was rarely positive (16%); then came grass pollens (46%), house dust mites (d. pteronyssinus, 38%) and animal danders (cat 3 ... | 1986 | 3764390 |
| cultivation of fungi in synthetic and semi-synthetic liquid medium. ii. immunochemical properties of the antigenic and allergenic extracts. | four allergologically important fungi, viz. aspergillus fumigatus, penicillium notatum, alternaria alternata, and cladosporium herbarum were cultured in a liquid synthetic medium, with or without addition of 0.1% yeast extract (ye). after 10 and 28 days of cultivation, immunochemical properties of the fungal extracts, characterised by precipitation pattern and igg- and ige-binding capacity, were studied. in pure synthetic medium no large differences were observed in number of precipitates, as me ... | 1985 | 3911818 |
| diagnosis and immunotherapy of mould allergy. i. screening for mould allergy. | in order to screen for mould allergy, extracts of five common atmospheric moulds (cladosporium, alternaria, penicillium, aspergillus and mucor) from various manufacturers were investigated in 130 patients (5-60 years old) with clinical symptoms indicating possible mould allergy. the patients were screened by skin prick test (spt) and radioallergosorbent test (rast). spt seemed to be more sensitive than rast as a diagnostic screening procedure (80% positive reactions to one or more species compar ... | 1985 | 3993865 |
| purification and partial characterization of the allergen ag-54 from cladosporium herbarum. | one important allergen, ag-54, was extracted from cladosporium herbarum and purified by a combination of diafiltration, gel filtrations and isoelectric focusing. the allergen, when examined by gel electrophoresis and high-performance liquid chromatography, was found to be essentially homogeneous with a molecular weight of about 20,000 daltons. ag-54 is of glycoprotein nature. it contained 19.5% protein, calculated from its amino acid composition and 80.2% carbohydrate, quantified by methanolysis ... | 1985 | 4055077 |
| cultivation of fungi in synthetic and semi-synthetic liquid medium. i. growth characteristics of the fungi and biochemical properties of the isolated antigenic material. | four allergologically important fungi, viz. aspergillus fumigatus, alternaria penicillium notatum, and cladosporium herbarum, were cultured in a pure synthetic medium and the patterns of growth as characterized by the ph, protein and carbohydrate concentration of the culture fluid, were studied. a fumigatus and p. notatum showed a similar growth pattern, characterized by a rapid decrease in the ph of the culture medium (ph 7.4----4.0), while proteins were slowly released and saccharose poorly co ... | 1985 | 4091234 |
| pathogenic effects of cladosporium herbarum and penicillium decumbens. | | 1973 | 4203154 |
| lesions experimentally produced by fungi implicated in extrinsic allergic alveolitis. | the ability of aureobasidium pullulans, cladosporium herbarum and cryptostroma corticale to produce local lesions in the rabbit was examined. both c. corticale and a. pullulans can survive in vivo and produce the inflammatory response typical of mycetoma. c. herbarum failed to grow or survive in vivo. | 1974 | 4531447 |
| influence of nitrogen source on the synthesis of fat from sucrose by aspergillus terreus, aspergillus ochraceus, cladosporium fulvum, cladosporium herbarum and penicillium gladioli. | | 1972 | 4672593 |
| cutaneous staphylococcosis and secondary infection of house sparrow with the fungus cladosporium herbarum. | | 1974 | 4836176 |
| effects of n2-o2 and co2-o2 tensions on growth of fungi isolated from damaged flue-cured tobacco. | ten fungi, aspergillus niger, a. flavus, a. ochraceus, a. ruber, a. repens, a. amstelodami, alternaria tenuis, penicillium brevi-compactum, cladosporium herbarum, and chaetomium dolicotrichum, were isolated from moldy flue-cured tobacco and grown in various mixtures of n(2)-o(2) or co(2)-o(2). a 1 to 5% concentration of o(2) in an n(2) atmosphere caused the greatest change in growth of the nine species, and a 10 to 20% concentration of o(2) for a. flavus. all species, except a. amstelodami and a ... | 1970 | 5461786 |
| alpha-amylase inhibitor from fungus cladosporium herbarum f-828. | a strain of fungus cladosporium herbarum extracellularly produced an inhibitor specific for mammalian alpha-amylase. the inhibitor was purified 81-fold by freeze-thawing, heat treatment, and column chromatography on deae-cellulose, sephadex g-75, deae-sephacel, and bio-gel p-100. an apparent molecular weight of approximately 18,000 was estimated for the inhibitor using bio-gel p-100 filtration. the purified inhibitor preparation was a glycoprotein containing about 10% carbohydrate. the amino aci ... | 1982 | 6174515 |
| respiratory virus infections and aeroallergens in acute bronchial asthma. | two hundred and fifty six attacks of acute bronchial asthma occurring in 169 children aged over 2 years were studied during a two year period. more attacks occurred during spring and autumn than at other times of the year. in 73 patients (29%) a respiratory virus infection was diagnosed, with the same seasonal variation as the asthmatic attacks. most of the virus infections were caused by rhinovirus (45%) and respiratory syncytial virus (19%). there was no significant correlation between asthmat ... | 1984 | 6326694 |
| variability in different strains of cladosporium herbarum with special attention to carbohydrates and contents of two important allergens (ag-32 and ag-54). | ten different strains of cladosporium herbarum cultivated and extracted under identical experimental conditions were compared with respect to: 1) carbohydrates quantified by methanolysis and gas-liquid chromatography, 2) contents of two important cladosporium allergens ag-32 and ag-54 estimated by rocket radioimmunoelectrophoresis, 3) protein content determined by the lowry and bradford methods, respectively, and 4) allergenic activity as measured by rast. the allergen extracts contained 45-73% ... | 1984 | 6540533 |
| standardization of allergen extracts by inhibition of rast, skin test, and chemical composition. | five allergen extracts of dermatophagoides pteronyssinus, lolium perenne, alternaria tenuis, aspergillus fumigatus and cladosporium herbarum, obtained from four different manufacturers, were examined by inhibition of rast, content of protein and carbohydrate, contents of phosphorylcholine (pc) and tridacnin reactive components, and by skin test. inhibition of rast was used as a primary method for establishing allergenic potency and demonstrated wide variations for each preparation supplied by th ... | 1980 | 7004662 |
| purification of allergens in cladosporium herbarum. | | 1980 | 7189972 |
| immediate type hypersensitivity to common moulds. comparison of different diagnostic materials. | extracts of five common atmospheric moulds (alternaria, aspergillus, cladosporium, mucor and penicillium) obtained from different manufacturers were compared by means of skin prick testing and, partly, with the radioallergosorbent test. considerable differences were found between the extracts with respect to positive tests found in mould-allergic individuals and controls. a purified allergen preparation of cladosporium herbarum was superior to the crude allergen extracts. the results suggest tha ... | 1980 | 7192501 |
| the prevalence of immediate positive skin tests in nepalese children. | two hundred and ninety-three normal schoolchildren between 5 and 15 years old living in the hills of eastern nepal were tested by the skin prick method for sensitivity to six allergens: dermatophagoides pteronyssinus, aspergillus fumigatus. cladosporium herbarum, penicillium notatum, mixed pollens and mixed threshings. these children were also questioned and examined for symptoms and signs relating to allergic disease. of the children, 20% were skin-prick positive to at least one of the allergen ... | 1981 | 7249341 |
| [mould allergy (author's transl)]. | 290 asthmatic patients with positive skin tests for mould allergens were analyzed retrospectively. the relationship between skin test and inhalative provocation test was investigated with regard to single allergens and polyvalent mould sensitivity. with 4 commercially available moulds the relationship between skin test, provocation test and rast was investigated too. alternaria alternata occupies a special position. tests with alternaria alternata, aureobasidium pullulans, penicillium notatum, f ... | 1980 | 7395259 |
| rhizosphere fungi of five plants in egypt. | 75 species which belong to 25 genera were collected during this investigation. all of these fungi were previously isolated from egyptian soils and other sources. the total count and the number of genera and species in the rhizosphere soil of the 5 plants were regularly higher than in non-rhizosphere soil. the composition of the rhizosphere fungus flora was considerably influenced by the type and the age of the plant. the most prevalent fungi in the rhizosphere of the test plants after 45, 90, an ... | 1980 | 7424047 |
| antifungal compounds induced in the dual culture with phytolacca americana callus and botrytis fabae. | in order to investigate new metabolites which are only induced in a plant callus infected by a pathogenic fungus, dual cultures with combinations of 10 species of fungi and 6 plant cell lines from different species were established. among the combinations tested, the methanolic extract of a dual culture consisting of a plant cell line, phytolacca americana and a fungus, botrytis fabae showed a marked antifungal activity to cladosporium herbarum. the main active constituent of this extract was id ... | 1995 | 7546032 |
| airborne fungal load in agricultural environment during threshing operations. | the effect of threshing operations on the fungal spora of farm air, has been studied with the culture plate method. air samples were taken before, during and after the threshing of paddy and wheat grains, at the end of the respective crop seasons. an immediate local increase in the airborne fungal load was observed during threshing operations. in case of wheat the high rise was due to alternaria tenuissima, followed by drechslera sp. and cladosporium herbarum. in 'boro' variety of paddy, the hig ... | 1994 | 7808509 |
| a cdna clone coding for a novel allergen, cla h iii, of cladosporium herbarum identified as a ribosomal p2 protein. | mold allergens represent a major cause of atopic disorders. progress in the molecular characterization of allergens has been hampered by batch-to-batch variation and poor yields in mold extracts. in the present study, we established a cdna library in lambda zap ii by using mrna isolated from a major allergen-producing mold, cladosporium herbarum. from this library, a novel allergen has been cloned and sequenced. the clone encodes a full length protein of 111 amino acids with a molecular mass of ... | 1995 | 7814877 |
| [contamination of archives by filamentous fungi and their evaluation for potential pathogenicity]. | the results of microbiological examinations of dust samples collected from mortgage registers in the court archives are presented. in the samples examined filamentous fungi of penicillium genus were observed as well as cladosporium herbarum, geotrichum candidum, cephalosporium glutineum, mucor racemosus, trichoderma viride and aspergillus niger. filamentous fungi of penicillium genus occurred in the highest concentration accounting for 350,000 cfu/g. the levels of the remaining fungi were lower- ... | 1994 | 7854106 |
| molecular cloning of major and minor allergens of alternaria alternata and cladosporium herbarum. | the two moulds, alternaria alternata and cladosporium herbarum, are recognized as major causes of fungal allergies. cloning, sequencing and heterologous expression of the allergens of the two moulds is a necessary step in understanding fungal allergy and in the development of new and improved methods of diagnosis and therapy. the seven new mould allergens presented here represent four new allergen proteins: aldehyde dehydrogenase (aldh), enolase, ycp4 (previously found as a saccharomyces cerevis ... | 1995 | 7898496 |
| fungal production of volatiles during growth on fiberglass. | acoustic and thermal fiberglass insulation materials used in heating, ventilation, and air-conditioning systems were colonized with fungi in laboratory chambers. the mixed fungal population, principally aspergillus versicolor, acremonium obclavatum, and cladosporium herbarum, produced odoriferous volatiles, including 2-ethyl hexanol, cyclohexane, and benzene. these volatiles may be related to poor indoor air quality and the sick building syndrome. | 1994 | 7993098 |
| two-dimensional immunoblot analysis of allergens of cladosporium herbarum. | in the present study, allergens of cladosporium herbarum were analysed by sds-page, two-dimensional gel electrophoresis and immunoblot. the sds-page under reduced conditions followed by western blotting analysis revealed 10 human ige-binding components with molecular masses ranging from 15 to 143 kda. within the same molecular mass range, five components were detected under non-reduced conditions. examination of the specificities of 10 individual sera revealed that two patients were sensitive to ... | 1994 | 8012858 |
| [current aspects of fungal spores allergy]. | in industrialized countries the prevalence of allergic inhalant diseases is some 15-20%. more than 10% of these individuals are sensitized to fungal allergens. many fungal spores are less than 10 microns in size, which permits penetration into the smaller airways of the lung. bronchial provocation tests have demonstrated that fungal spores and spore extracts can cause both an early and a late phase reaction in sensitive subjects. over 80 genera of fungi have been associated with symptoms of resp ... | 1994 | 8016603 |
| [chromomycosis: 2 cases]. | we report two cases of chromomycosis due to fonsecaea pedrosoi and cladosporium herbarum, observed in two women from the tlemcen region. due to the wart-like or nodular lesions, these case were mistaken for other skin diseases with similar manifestations, particularly verrucous tuberculosis and cutaneous leishmaniasis. the histology revealed fumagoid bodies leading to the identification of the causative agents. chromomycosis is rare in algeria although 3 cases have been observed in our area sinc ... | 1994 | 8092726 |
| investigation of possible adverse allergic reactions to mycoprotein ('quorn'). | mycoprotein ('quorn') is a food produced for human consumption from fusarium graminearum. crossreactivity studies showed that mycoprotein shared multiple common allergenic determinants with aspergillus fumigatus and cladosporium herbarum and some with alternaria alternata. there is, therefore, a potential for mould allergic patients to react adversely to inhaled or ingested mycoprotein. mycoprotein rast screening of mycoprotein production workers was made during a 2 year period. two of the produ ... | 1993 | 8319120 |
| new cause for false-positive results with the pastorex aspergillus antigen latex agglutination test. | the pastorex aspergillus antigen test for detection of aspergillus galactomannan antigen in the sera of patients with invasive aspergillosis is used in many clinical laboratories. a serum sample contaminated with penicillium chrysogenum gave a strongly positive reaction (1:128) which was heat stable, was not eliminated by pronase treatment, and was not detected by a normal rabbit globulin control. this observation was shown to be due to cross-reactions of the monoclonal antibody eb-a2 used by th ... | 1993 | 8408572 |
| studies on shared antigenic/allergenic components among fungi. | fungal allergens have been found to be one of the most prevalent aeroallergens in india. knowledge of shared/unique components among different fungi is necessary for proper diagnosis and treatment of patients allergic to fungi. in the present study, crude extracts (ce) of 11 common fungi (alternaria alternata, aspergillus flavus, asp. fumigatus, asp. niger, asp. tamarii, asp. versicolor, cladosporium herbarum, curvularia lunata, mucor hiemalis, penicillium citrinum, and fusarium solani) were cha ... | 1995 | 8607563 |
| development of an oligonucleotide probe for aureobasidium pullulans based on the small-subunit rrna gene. | aureobasidium pullulans, a cosmopolitan yeast-like fungus, colonizes leaf surfaces and has potential as a biocontrol agent of pathogens. to assess the feasibility of rrna as a target for a. pullulans-specific oligonucleotide probes, we compared the nucleotide sequences of the small-subunit rrna (18s) genes of 12 geographically diverse a. pullulans strains. extreme sequence conservation was observed. the consensus a. pullulans sequence was compared with other fungal sequences to identify potentia ... | 1996 | 8633850 |
| an allergenic polypeptide representing a variable region of hsp 70 cloned from a cdna library of cladosporium herbarum. | extracts of cladosporium herbarum, a major source of fungal aeroallergens, exhibit a complex profile of ige-binding proteins. yields of conventionally purified allergens from this mold have been insufficient to permit further molecular analyses. | 1996 | 8789547 |
| sensitization to alternaria and cladosporium by the age of 4 years. | of 1218 children born on the isle of wight in 1989/90, and followed for atopy at age 4 years, 981 were skin-prick tested with a battery of allergens. of these 61 (6%) reacted positively to alternaria alternata and cladosporium herbarum (47 to alternaria, 21 to cladosporium and seven to both). twenty-four (39%) were asymptomatic (latent atopy) of which 12 had a single positive reaction either to alternaria or cladosporium. asthma was the most common disease in children sensitized to moulds. alter ... | 1996 | 8842553 |
| sensitization to neumoallergens influence in mothers of asthmatic children. | allergy diseases in our province have the characteristic of early appearance of asthma and predominant monosensitization to domestic dust mites due to the climatic condition. in this work we study the influence in allergic children. we selected 1028 mothers that brought their children to hospital for the first time. we divided them in two groups. one was comprised of 459 mothers of atopic children (ma) and another of 569 mothers of non atopic children (mc). all mothers were skin-prick tested (ph ... | 1996 | 8882754 |
| the distribution of total and specific serum ige in the european community respiratory health survey. | variations in the prevalence of atopy could provide important clues to the etiology of atopy and asthma. although estimates of prevalence are available from different studies, a lack of standardization makes comparisons difficult. | 1997 | 9058686 |
| allergen content in dust from homes and schools in northern norway in relation to sensitization and allergy symptoms in schoolchildren. | previous studies have shown a high prevalence of atopic diseases among school children in the community of sør-varanger. moreover, animal dander followed by pollen and house dust mite, were the most common allergens in skin prick tests. | 1997 | 9088651 |
| molecular characterization of alternaria alternata and cladosporium herbarum allergens. | | 1996 | 9095236 |
| enolases are highly conserved fungal allergens. | lack of knowledge of the identity of fungal allergens still is a major obstacle for improvement of diagnosis and therapy of allergies to moulds. we have therefore further analyzed the allergens of the two moulds, alternaria alternata and cladosporium herbarum and found that enolases (ec 4.2.1.11) are major allergens, at least of the two fungal species just mentioned. | 1997 | 9130497 |
| fungi associated with post-harvest rot of black plum (vitex doniana) in nigeria. | the fungi associated with rot of vitex doniana fruits (blackplum) were isolated and identified. aspergillus niger, botryodiplodia theobromae, candida spp. penicillium chrysogenum, rhizopus stolonifer, fusarium pallidoroseum f. oxysporum and mucor mucedo were the primary rot causing fungi in contrast to cladosporium herbarum and mucor circinelloides which were just present as secondary colonizers. the rot fungi penetrated mainly through wounds and bruises on the surface of fruits. mature green fr ... | 1996 | 9208478 |
| prenylated phenylpropenes from coleonema pulchellum with antimicrobial activity. | the lipophilic root extract of coleonema pulchellum was analysed and tested for antifungal and antibacterial activity. eight previously undescribed prenyloxy and geranyloxy phenylpropenes, were isolated as major compounds together with the known evofolin-c as well as the lignans (+/-)-sesamin and (+/-)-prenylpiperitol, the diterpene (-)-pimara-9(11),15-dien-19-oic acid and the 2,4-decadienoic acid isobutylamide. all structures were established by spectroscopic evidence. from the new phenylpropen ... | 1997 | 9272968 |
| the antimicrobial activity of 3,5,7-trihydroxyflavone isolated from the shoots of helichrysum aureonitens. | extracts from helichrysum aureonitens are used topically by the indigenous people of south africa against infections. the antimicrobial activity-guided fractionation by bioautography of the acetone extract from the aerial parts of h. aureonitens led to the isolation of 3,5,7-trihydroxyflavone (galangin). evaluation of the antibacterial activity of the compound against ten randomly selected bacteria indicated significant activity against all the gram-positive bacteria tested with the minimum inhi ... | 1997 | 9292410 |
| endoglucanase production by paper-degrading mycoflora. | fourteen fungal species, namely aspergillus flavus, a. fumigatus, a. niger, a. ustus, penicillium islandicum, p. wortmannii, memnoniella echinata, cladosporium herbarum, stachybotrys atra, chaetomium globosum, fusarium oxysporum, torula herbarum, alternaria alternata and curvularia uncinata were isolated from different grades of paper. they differ in their distribution on various kinds of paper and also in relative occurrence. while seasonal influence on mycoflora was observed, most of the mould ... | 1997 | 9418063 |
| biodegradation of anthracene by soil fungi. groupe pour l'etude du devenir des xénobiotiques dans l'environnement (gedexe). | a selection of 39 strains of micromycetes known as good degraders of polychlorinated aromatic compounds, mostly isolated from soil and belonging to various taxonomic groups, have been investigated for anthracene degradation. toxicity and consumption assays, first evaluated on solid media, have not shown any toxicity of anthracene (1-100 mg.l-1) towards fungi. degradation of anthracene (10 mg.l-1) was then investigated in a liquid synthetic medium for 4 days and evaluated by hplc. among the 39 st ... | 1998 | 9661278 |
| molecular cloning of ige-binding fragments of alternaria alternata allergens. | exposure to the hyphomycete alternaria alternata is recognized as an important risk factor for asthma. ige immunoblotting has been used to catalogue the number and mr of allergens in a. alternata extracts, with estimates ranging from 10 to 30, although few are present in nearly all extracts studied. several a. alternata allergens have been cloned, including a subunit of the major allergen alt a 1, ribosomal p2 phosphoprotein, aldehyde dehydrogenase and a yeast ycp4 homolog. we have cloned two se ... | 1998 | 9693275 |
| identification, n-terminal region sequencing and similarity analysis of differentially expressed proteins in paracoccidioides brasiliensis. | paracoccidioides brasiliensis is the causal agent of paracoccidioidomycosis, which is a systemic mycosis in latin america. this human pathogen is a dimorphic fungus existing as mycelium (26 degrees c) and in infected tissues as a yeast form (36 degrees c). the in vitro differentiation process is reversible and dependent on temperature shift. in the present study, the total proteins from both forms of p. brasiliensis (isolate pb01) were analysed by two-dimensional electrophoresis. differentially ... | 1999 | 10361267 |
| antifungal effects of different plant extracts and their major components of selected aloe species. | different extracts of both fresh and dry leaves of aloe eru a. berger, a. vera l. webb & berth and a. arborescens mill. were screened for their antifungal activity against aspergillus niger, cladosporium herbarum and fusarium moniliforme. the toxicity of the isolated pure components were evaluated on the tested fungi. a comparative chromatographic study was performed to differentiate between natural components existing in various fractions and extracts of aloe species and specific spray reagents ... | 1999 | 10441780 |
| evaluation of different methods for the extraction of dna from fungal conidia by quantitative competitive pcr analysis. | five different dna extraction methods were evaluated for their effectiveness in recovering pcr templates from the conidia of a series of fungal species often encountered in indoor air. the test organisms were aspergillus versicolor, penicillium chrysogenum, stachybotrys chartarum, cladosporium herbarum and alternaria alternata. the extraction methods differed in their use of different cell lysis procedures. these included grinding in liquid nitrogen, grinding at ambient temperature, sonication, ... | 1999 | 10445315 |
| association between severity of atopic eczema and degree of sensitization to aeroallergens in schoolchildren. | a subgroup of patients with atopic eczema exhibits aggravation through contact with aeroallergens. little is known from population-based studies, however, about the association between the severity of eczematous skin disease and the degree of aeroallergen sensitization. | 1999 | 10589013 |
| antifungal nitro compounds from skunk cabbage (lysichitum americanum) leaves treated with cupric chloride. | two nitro compounds, 2-(4-methoxyphenyl)-1-nitroethane named as lysichitalexin and 2-(4-hydroxyphenyl)-1-nitroethane were isolated as stress metabolites from the leaves of lysichitum americanum hultén and st. john treated with cupric chloride. their structures were determined by spectroscopic methods and chemical reactions. the former compound showed antifungal activities against fusarium oxysporum and cladosporium herbarum. both compounds were isolated for the first time from this species and t ... | 2000 | 10656408 |
| [factors associated with the development of atopy in young adults]. | to determine the risk factors for the development of atopy in spanish young adults. | 2000 | 10738720 |
| evaluation of the mycological status of luncheon meat with special reference to aflatoxigenic moulds and aflatoxin residues. | the luncheon meat samples analyzed, which were produced locally by the two main luncheon meat producing companies in egypt were relatively highly contaminated either by moulds and yeasts in general, aflatoxigenic species and aflatoxin residues in particular. the most frequently encountered fungi from the samples were yeasts, aspergillus niger, a. flavus, penicillium chrysogenum, rhizopus stolonifer, mucor circinelloides. less common were cladosporium sphaerospermum, alternaria alternata, mycosph ... | 1999 | 10823186 |
| protease-dependent activation of epithelial cells by fungal allergens leads to morphologic changes and cytokine production. | proteases in extracts of aspergillus fumigatus cause epithelial cell desquamation and release of proinflammatory cytokines. | 2000 | 10856154 |
| traffic exposure and allergic sensitization against latex in children. | latex antigens have been found in urban air samples and in debris deposited near freeways. | 2000 | 10984380 |
| ige-binding epitopes of enolases, a class of highly conserved fungal allergens. | cladosporium herbarum and alternaria alternata are two of the most prominent fungal species inducing type i allergy. previously, we have demonstrated that enolase (cla h 6) is the second most important allergen of c herbarum in terms of frequency of sensitization. | 2000 | 11080711 |
| hev b 9, an enolase and a new cross-reactive allergen from hevea latex and molds. purification, characterization, cloning and expression. | natural rubber latex allergy is an ige-mediated disease that is caused by proteins that elute from commercial latex products. a complementary dna (cdna) coding for hev b 9, an enolase (2-phospho-d-glycerate hydrolyase) and allergen from latex of the rubber tree hevea brasiliensis, was amplified by pcr. the pcr primers were designed according to conserved regions of enolases from plants. the obtained cdna amplification product consisted of 1651 bp and encoded a protein of 445 amino-acid residues ... | 2000 | 11106410 |
| tapping allergen repertoires by advanced cloning technologies. | complex allergenic sources such as moulds, foods and mites contain complex panels of ige-binding molecules which need to be cloned, produced and characterized in order to mimic the entire allergenicity of whole extracts reconstituted by mixing single standardized recombinant allergens. | 2001 | 11306922 |
| new macrolides and furan carboxylic acid derivative from the sponge-derived fungus cladosporium herbarum. | bioassay-guided fractionation of organic extracts of cladosporium herbarum, isolated from the marine sponge callyspongia aerizusa, yielded two new macrolide metabolites: pandangolide 3 and 4 (1 and 2) and the known fungal metabolites pandangolide 2 (3), cladospolide b (4), and iso-cladospolide b (5). also isolated were the antimicrobially active (against bacillus subtilis and staphylococcus aureus) furan carboxylic acids: sumiki's acid (6) and its new derivative, acetyl sumiki's acid (7). all st ... | 2001 | 11325242 |
| [animals and fungi as allergy inducers]. | pets particularly dog and cat are the men's best friend. in the daily practice respiratory allergy to animal proteins are not uncommon and in some areas the frequency is even higher than allergy to house dust mites. in switzerland nearly half of the households keeps some kind of a domestic animal with cats followed by dogs as the principal pets. because the exposure to domestic animals is perennial, allergic symptoms such as rhinoconjunctivitis or asthma usually are less acute than due to pollen ... | 2001 | 11407231 |
| antimicrobial cuparene-type sesquiterpenes, enokipodins c and d, from a mycelial culture of flammulina velutipes. | two new cuparene-type sesquiterpenes, enokipodins c (1) and d (2), were isolated from culture medium of an edible mushroom, flammulina velutipes, along with enokipodins a (3) and b (4). the structures of 1 and 2 were determined using spectroscopic methods (hrms, (1)h and (13)c, and 2d nmr). the absolute configuration of enokipodin c was determined from the observed (1)h nmr chemical shifts and noes in noesy experiments after conversion into the corresponding esters with the chiral reagent 2-(2'- ... | 2001 | 11473426 |
| sensitization to common allergens and its association with allergic disorders at age 4 years: a whole population birth cohort study. | atopy is defined as the genetic propensity to develop immunoglobulin e antibodies in response to exposure to allergens and assessed by skin prick test responses to common allergens. although it is generally agreed that atopy is an important risk factor for allergic diseases such as asthma, rhinitis, and eczema, the extent to which atopy accounts for these diseases is controversial. | 2001 | 11483843 |
| fungal flora of the digestive tract of 5 species of triatomines vectors of trypanosoma cruzi, chagas 1909. | a study of the mycobiota in the digestive tract of 5 important species of triatomines, triatoma brasiliensis, t infestans, t. sordida, t. pseudomaculata and t. vitticeps, was made. the digestive tracts of 164 adults and 535 nymphs of those triatomines were studied and 393 fungal strains were isolated. the genera with the greatest number of species were penicillium (19 species), aspergillus (17 species) and acremonium (5 species) and the most frequent species, in decreasing order, were penicilliu ... | 2001 | 11502063 |
| stress induced carbazole phytoalexins in glycosmis species. | induced formation of a series of carbazole alkaloids was observed in leaves of glycosmis parviflora and g. pentaphylla after wounding, uv-irradiation, and particularly after inoculation with the fungus botrytis cinerea. chemical variation between different provenances and even individuals of g. parviflora led to an accumulation of different derivatives from which three proved to be undescribed natural products. their structures were identified by spectroscopic methods and named carbalexins a, b, ... | 2001 | 11524122 |
| prevalence of immunoglobulin e for fungi in atopic children. | the prevalence of sensitization to fungi in young atopic patients in relation to age and clinical importance is largely unknown. | 2001 | 11678856 |
| unfolding and double-stranded dna binding of the cold shock protein homologue cla h 8 from cladosporium herbarum. | the cloning, purification, and biophysical characterization of the first eukaryotic cold shock protein homologue, cla h 8, expressed as single functional polypeptide is reported here. it was discovered as a minor allergen of the mold cladosporium herbarum by phage display using a library selectively enriched for ige-binding proteins. based on the sequence homology of cla h 8 with bacterial cold shock proteins (csps), a homology-based computer model of the allergen was computed indicating an all- ... | 2002 | 11861653 |
| new metabolites from sponge-derived fungi curvularia lunata and cladosporium herbarum. | the fungus curvularia lunata, isolated from the marine sponge niphates olemda, yielded the new 1,3,8-trihydroxy-6-methoxyanthraquinone, which we named lunatin (1), the known modified bisanthraquinone cytoskyrin a (2), and the known plant hormone (+)-abscisic acid (3). both anthraquinones were found to be active against bacillus subtilis, staphylococcus aureus, and escherichia coli. two strains of the fungus cladosporium herbarum, isolated from the sponges aplysina aerophoba and callyspongia aeri ... | 2002 | 12027752 |
| increase in the prevalence of allergen skin sensitization in successive birth cohorts. | little is known about longitudinal trends in the prevalence of allergen skin sensitization in the general population. | 2002 | 12063526 |
| antifungal garcinia acid esters from the fruits of garcinia atroviridis. | two new garcinia acid derivatives, 2-(butoxycarbonylmethyl)-3-butoxycarbonyl-2-hydroxy-3-propanolide and 1',1"-dibutyl methyl hydroxycitrate, were isolated from the fruits of garcinia atroviridis guided by tlc bioautography against the fungus cladosporium herbarum. the structures of these compounds were established by spectral analysis. the former compound represents a unique beta-lactone structure and the latter compound is most likely an artefact of garcinia acid (= hydroxycitric acid). both c ... | 2002 | 12064729 |