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fermentation and aerobic metabolism of cellodextrins by yeasts.the fermentation and aerobic metabolism of cellodextrins by 14 yeast species or strains was monitored. when grown aerobically, candida wickerhamii, c. guilliermondii, and c. molischiana metabolized cellodextrins of degree of polymerization 3 to 6. c. wickerhamii and c. molischiana also fermented these substrates, while c. guilliermondii fermented only cellodextrins of degree of polymerization less than or equal to 3. debaryomyces polymorphus, pichia guilliermondii, clavispora lusitaniae, and one ...19912039228
disseminated fungal infections in children infected with human immunodeficiency virus.a retrospective review of charts of 156 human immunodeficiency virus-infected children cared for during a 7.5-year period revealed 11 episodes of disseminateed candidiasis (dc) occurring in 11 patients (7%). all 11 patients developed the fungal infection in the context of advanced human immunodeficiency virus infection. all but one were hospital-acquired, occurring at a mean of 2.3 months after admission. ten patients had been febrile for more than 14 days before diagnosis. previous oral thrush ...19911766703
yeasts and fungi occurring in ensiled whole-crop maize and other ensiled vegetable crops.the yeast flora of whole-crop maize ensiled for two weeks was predominated by candida holmii, c. lambica, c. milleri, hansenula anomala and saccharomyces dairensis. inoculation with other yeast species reported in the literature to prevail in maize or wheat silages did not alter the yeast flora. at 25 or 30 degrees c the ascomycetous fermentative species found at 20 degrees c were accompanied with ascomycetous non-fermentative fungi, i.c. exophiala jeanselmei and verticillium psalliotae, by the ...19902321936
evaluation of the updated vitek yeast identification data base.using 398 isolates of yeasts and yeastlike fungi comprising 9 genera and 26 species, as well as the hyphomycete geotrichum candidum and the achlorophyllous alga prototheca wickerhamii, we compared the api 20c yeast identification system with the modified vitek yeast identification system with an expanded data base. we found 11 discrepancies between the two systems: five (1.3%) of the isolates (blastoschizomyces capitatus, 1; candida albicans, 1; hansenula anomala, 1; rhodotorula minuta, 2) had b ...19902229376
limited multiplication of mycobacterium lepraemurium in parabiotic culture, as influenced by osmolarity of an alkaline-galactomannan medium.kato, laszlo (institut de microbiologie et d'hygiène de l'université de montréal, montreal, quebec, canada), and bela gozsy. limited multiplication of mycobacterium lepraemurium in parabiotic culture, as influenced by osmolarity of an alkaline-galactomannan medium. j. bacteriol. 91:1859-1862. 1966.-limited multiplication of mycobacterium lepraemurium has been achieved in an alkaline (ph 8.4) galactomannan-containing medium, when cultivated alone or in parabiosis with a feeder strain, torula minu ...19665937242
airborne fungi in the air of barcelona, spain. v. the yeasts.during a two-year survey of the fungal flora present in the air of barcelona, spain, the following species of yeasts were isolated: sporobolomyces roseus kluyver et van niel, candida albicans (robin) berkhout, rhodotorula glutinis (fres.). harrison var. glutinis and rhodotorula minuta (saito) harrison. diurnal and seasonal incidences are presented.19806996535
distribution of aspartate aminotransferase activity in yeasts, and purification and characterization of mitochondrial and cytosolic isoenzymes from rhodotorula minuta [corrected].the distribution of aspartate aminotransferase activity in yeasts was determined. the number of species of the enzyme in each yeast was determined by zymogram analysis. all the yeasts, except for the genus saccharomyces, showed two or three activity bands on a zymogram. from among the strains, rhodotorula minuta [corrected] and torulopsis candida were selected for examination of the existence of yeast mitochondrial isoenzymes, because these strains showed two clear activity bands on the zymogram ...19902332417
nadh: ubiquinone oxidoreductase in obligate aerobic yeasts.the strictly aerobic yeasts candida pinus, cryptococcus albidus, rhodotorula minuta, rhodotorula mucilaginosa and trichosporon beigelii possess mitochondrial nadh dehydrogenases with significant features of the nadh:ubiquinone oxidoreductase (complex i). these species show in all growth phases and under standard cultivation conditions, nadh dehydrogenases of approximately 700 kda, which are sensitive to rotenone, a specific inhibitor of this complex. identical results were obtained with the weak ...19947941733
reconstitution of the isobutene-forming reaction catalyzed by cytochrome p450 and p450 reductase from rhodotorula minuta: decarboxylation with the formation of isobutene.an isobutene-forming activity was reconstituted with cytochrome p450rm and cytochrome p450 reductase purified from rhodotorula minuta. the nonionic detergent. emulgen 911, present in the preparation of purified p450rm, inhibited the reconstitution. bovine serum albumin enhanced the activity of the reconstituted system. branching of the beta carbon of the substrate carboxylic acid was important for formation of isobutene. in a comparative study with isovalerate and 3-deuterio-3-methylbutanoate, a ...19948002981
production of galacto-oligosaccharide from lactose by sterigmatomyces elviae cbs8119.our stock cultures were screened for microorganisms that can produce galacto-oligosaccharide (gal-os) from lactose. of the 574 strains of bacteria and yeasts tested, sterigmatomyces elviae cbs8119, rhodotorula minuta ifo879, and sirobasidium magnum cbs6803 were found to be efficient producers of gal-os from lactose and s. elviae cbs8119 was selected as a representative, high-level producing strain. with toluene-treated resting s. elviae cbs8119 cells, 135 mg of gal-os per ml was produced from 36 ...19958526516
central venous catheter infection with rhodotorula minuta in a patient with aids taking suppressive doses of fluconazole.a case of rhodotorula minuta central venous catheter infection with fungaemia is described in a patient with advanced acquired immunodeficiency syndrome (aids), hiv nephropathy, end-stage renal disease requiring haemodialysis, and a permanent quinton catheter in place for 6 months. at the time of fungaemia, the patient was taking 100 mg fluconazole per os daily for a previous episode of candida oesophagitis. r. minuta central venous catheter infection with fungaemia was successfully treated with ...19958531026
cytochrome p450rm from rhodotorula minuta catalyzes 4-hydroxylation of benzoate.rhodotorula minuta, a red yeast, produces a cytochrome p450, tentatively named p450rm, catalyzing the formation of isobutene from isovalerate. we found that p450rm interacted with benzoate and the dissociation constant of p450rm for benzoate was 36 microm. a reconstituted system that consisted of purified p450rm and cytochrome p450 reductase catalyzed the 4-hydroxylation of benzoate in addition to the formation of isobutene; the turnover rate was approximately 40 nmol/min/nmol p450rm. the p450rm ...19968882724
structural characterization of the gene and corresponding cdna for the cytochrome p450rm from rhodotorula minuta which catalyzes formation of isobutene and 4-hydroxylation of benzoate.cytochrome p450rm was previously isolated from the basidiomycete yeast rhodotorula minuta as a bifunctional enzyme with isobutene-forming and benzoate 4-hydroxylase activities. we cloned the gene and corresponding cdna for p450rm in order to characterize the enzyme in the context of fungal phylogeny and physiology. from the cdna sequence, p450rm was deduced to have 527 amino acids with a calculated molecular weight of 59136. p450rm shared 48% amino acid sequence identity with cyp53a1 from asperg ...19979349702
survey for alpha-(1 leads to 3)-glucanase activity among yeasts.activity of alpha-(1 leads to 3)-glucanase was found in species of cryptococcus, rhodotorula, and endomyces. observations on the expression and stability of this enzyme in rhodotorula minuta var. texensis was presented.197718442
utilization of low molecular weight aromatic compounds by heterobasidiomycetous yeasts: taxonomic implications.the utilization of low molecular weight aromatic compounds implies the operation of complex metabolic pathways. in order to investigate the taxonomic relevance of this property among heterobasidiomycetous yeasts, both at the species level and at higher taxonomic ranks, the capacity to assimilate twenty such compounds was tested in a total of 332 strains representing approximately 200 species. the substrates most frequently utilized were protocatechuic, caffeic, and p-hydroxybenzoic acids, wherea ...199910453477
allergenic cross-reactivity of yeasts.yeast allergen extracts of candida albicans, c. pseudotropicalis, c. krusei, c. parapsilosis, c. tropicalis, c. guilliermondi, c. humicola, c. norwegica, c. utilis, cryptococcus albidus, geotrichum candidum, pityrosporon pachydermatis, p. ovale, rhodotorula minuta, r. rubra, saccharomyces cerevisiae, torulopsis glabrata and trichosporon cutaneum were investigated regarding their common allergenic properties. the enzyme immunoassay (eia) using rabbit anti-candida albicans antiserum showed remarka ...19883287997
inhibitors of the lipoxygenase pathway block development of brugia malayi l3 in vitro.brugia malayi l3 molt to the l4 stage in serum-free cultures supplemented with arachidonic, linoleic, or linolenic acids and the basidiomycetous yeast rhodotorula minuta. these fatty acids are capable of entering the eicosanoid pathway of arachidonate metabolism, the pathway responsible for generating a number of biologically active mediators, including prostaglandins, leukotrienes, and lipoxins. to determine whether this pathway was required for l3 development, we added dual inhibitors of cyclo ...200111318551
endogenous rhodotorula minuta and candida albicans endophthalmitis in an injecting drug user. 200111426427
pyrene degradation by yeasts and filamentous fungi.the saprotrophic soil fungi fusarium solani (mart.) sacc., cylindrocarpon didymum (hartig) wollenw, penicillium variabile sopp. and the yeasts rhodotorula glutinis (fresenius) harrison and rhodotorula minuta (saito) harrison were cultured in mineral medium with pyrene. the remaining pyrene concentrations were periodically determined during 20 incubation days, using hplc. to assess the metabolism of pyrene degradation we added 0.1 microci of [4,5,9,10] 14c-pyrene to each fungi culture and measure ...200211843531
lipid composition of some yeast strains from livingston island, antarctica.yeast strains cryptococcus albidus, cryptococcus laurentii, rhodotorula minuta were isolated from a moss sample. candida oleophila and rhodotorula mucilaginosa were isolated from a soil sample taken from livingston island. antarctica. fatty acid, phospholipid, sterol and tocopherol composition was determined in separated lipid fraction after fermentation in a medium containing glucose, peptone and yeast extract. unsaturated fatty acids, mainly oleic (51-65%) and linoleic (9.5-16.8%), predominate ...200111899472
rhodotorula minuta: an unusual fungal infection in hip-joint prosthesis.fungal infections of total joint arthroplasty are uncommon complications, and most cases have been associated with candida species. in this article, we present a case in which a total hip arthroplasty was infected with rhodotorula minuta. intraoperative gram stain showed yeast formsand led to early detection and immediate treatment with amphotericin b and bacitracin. successful reimplantation was performed 12 months later. to our knowledge, this is the first reported case of arthroplasty infecti ...200211922456
water-in-oil macroemulsions sustain long-term viability of microbial cells in organic solvents.extremely stable water-in-oil macroemulsions have been obtained by dispersing water in isooctane in the presence of lecithin. either prokaryotic (escherichia coli) and eukaryotic (saccharomyces cerevisiae and rhodotorula minuta) cells hosted in these water-in-oil macroemulsions are viable for weeks despite the consistent excess of organic solvent (ranging from 70 to 84%, v/v) in these ternary systems. conjugation occurs upon mixing macroemulsions containing f(+) or f(-) escherichia coli strains, ...200312474255
two endophytic fungi in different tissues of scots pine buds (pinus sylvestris l.).two fungal species were isolated with different frequencies from pine tissue cultures originating from buds. one species was detected in 33.1% of the cultures initiated in march, and another was present in 1.7% of cultures initiated in june. based on analyses of phylogenetic and physiological characteristics these fungi were identified as hormonema dematioides (isolated in march) and rhodotorula minuta (isolated in june). probes targeted towards the 18s rrna of h. dematioides and r. minuta were ...200312481234
rhodotorula pinicola sp. nov., a basidiomycetous yeast species isolated from xylem of pine twigs.three pink-colored yeast strains 3-1-3, 10-3-3 and 19-3-3 were isolated from xylem of surface-sterilized twigs of pinus tabulaeformis collected from dongling mountain, beijing, in different seasons. these strains were identified as rhodotorula minuta (saito) f.c. harrison by conventional taxonomic characterization. however, molecular phylogenetic analysis based on internal transcribed spacer region (including 5.8s rdna) and large-subunit rdna d1/d2 domain sequences indicated that they represent ...200212702303
purification and characterization of cytochrome p450 from an isobutene-forming microorganism, rhodotorula minuta.a cytochrome p450 was purified from microsomes of rhodotorula minuta. the optical spectrum of the purified cytochrome was characteristic of a low-spin ferric heme protein. isovalerate caused a type i spectral change in it. the amino-terminal sequence of the cytochrome was different from those of other known microsomal cytochrome p450s. these results indicate that the cytochrome, which is tentatively named p450rm, is a novel species of cytochrome p450.19937764227
characterisation of yeasts isolated from deep igneous rock aquifers of the fennoscandian shield.the diversity of prokaryotes in the groundwater deep below the surface of the baltic sea at the aspö hard rock laboratory (hrl) in southeast sweden is well documented. in addition, there is some evidence that eukaryotes, too, are present in the deep groundwater at this site, although their origins are uncertain. to extend the knowledge of eukaryotic life in this environment, five yeast, three yeastlike, and 17 mold strains were isolated from aspö hrl groundwater between 201 and 444 m below sea l ...200314502418
constitutive and uv-inducible synthesis of photoprotective compounds (carotenoids and mycosporines) by freshwater yeasts.twelve yeasts isolated from lakes of northwestern patagonia, argentina, belonging to eight genera (sporobolomyces, sporidiobolus, rhodotorula, rhodosporidium, cystofilobasidium, cryptococcus, torulaspora, and candida) were analysed for their ability to produce photoprotective compounds. for this purpose, three laboratory experiments were performed to study the effect of photosynthetically active radiation (par) and par in combination with uv radiation (par + uvr) on the production of carotenoids ...200414993945
bud endophytes of scots pine produce adenine derivatives and other compounds that affect morphology and mitigate browning of callus cultures.endophytes are found in meristematic bud tissues of scots pine (pinus sylvestris l.) especially prior to growth, which would suggest their involvement in growth of the bud. to test this hypothesis, production of phytohormones by two bacterial (methylobacterium extorquens, pseudomonas synxantha) and one fungal endophyte (rhodotorula minuta) was studied by mass spectrometry. the most common gibberellins, auxins, or cytokinins were not detected in the fractions studied. instead, m. extorquens and r ...200415153198
mycosporine-glutaminol-glucoside, a uv-absorbing compound of two rhodotorula yeast species.high-performance liquid chromatography (hplc), alone or in combination with mass spectrometry (ms), was used to screen the basidiomycetous yeasts rhodotorula minuta and r. slooffiae isolated from lakes for the presence of uv-absorbing compounds. mycosporine-glutaminol-glucoside (maximum absorption, 310 nm), a uv-photoprotective mycosporine known in terrestrial fungi, was the major uv-absorbing compound found in these species. this is the first identification of a mycosporine in yeasts. the prese ...200415484286
seasonal variations in location and population structure of endophytes in buds of scots pine.we studied the location and distribution of a bacterial isolate, a mycobacterium sp., in buds of scots pine (pinus sylvestris l.). using a probe specific for the 16s rrna of the mycobacterium sp., the bacterium was found by in situ hybridization in the meristematic tissues of 40% of all bud samples examined. because we had previously found other bacterial and fungal endophytes in the meristematic tissues of scots pine buds, we studied their occurrence in buds during shoot development and dormanc ...200515631977
organisms in an aspirate from an ulcerated mass: etiologic agent or mass-querade?smears prepared from a fine-needle aspirate of an ulcerated, dermal mass between the digits of a 9-month-old bull terrier puppy revealed a moderately cellular, mixed-cell inflammation with numerous extracellular organisms. the organisms were 3-8 microm in length, curved to oval in shape, with prominent nuclei, and (retrospectively) a rare single, narrow-based bud. the cytologic diagnosis was mycotic dermatitis due to infection with an unusual yeast. histologic sections of the lesion showed pyogr ...200515902671
production of ethyl (r)-2-hydroxy-4-phenylbutanoate via reduction of ethyl 2-oxo-4-phenylbutanoate in an interface bioreactor.ethyl (r)-2-hydroxy-4-phenylbutanoate [(r)-ehpb], a useful intermediate for the synthesis of various anti-hypertension drugs, was produced via microbial reduction of ethyl 2-oxo-4-phenylbutanoate [eopb] in an interface bioreactor. rhodotorula minuta ifo 0920 and candida holmii kpy 12402 were selected as the best type culture and isolated yeasts, respectively. the highest enantiomeric excess of (r)-ehpb produced by r. minuta and c. holmii were 95 and 94%, respectively. c. holmii was used for the ...19989805378
cytochrome p450rm of rhodotorula functions in the beta-ketoadipate pathway for dissimilation of l-phenylalanine.the physiological function of a microsomal cytochrome p450, p450rm, from rhodotorula minuta was determined. we obtained a mutant, 5-286, which has a point mutation in the p450rm gene, resulting in loss of the activities of the isobutene-forming enzyme and benzoate 4-hydroxylase and the ability to grow on medium containing l-phenylalanine as the sole carbon source. mutant 5-286 spontaneously reverted to the wild-type at a frequency of approximately 10(-9)-10(-10). these results indicate that p450 ...200016232893
preparation of a cell-free, isobutene-forming system from rhodotorula minuta.the cell-free isobutene-forming system of rhodotorula minuta ifo 1102 was prepared. this system consisted of isovaleric acid, nadph, and a cell extract. the maximal activity obtained by using the described cell-free system was 17 nl/mg of protein per h. it appears that this cell-free isobutene-forming system operates in living cells of r. minuta.198816347568
mycosporines from freshwater yeasts: a trophic cul-de-sac?mycosporine-like amino-acids (maas) are found in aquatic bacteria, algae, and animals. a related compound, the mycosporine-glutaminol-glucoside (myc-glu-glu), has recently been reported in freshwater yeasts. although animals depend on other organisms as their source of maas, they can efficiently accumulate them in their tissues. in this work we assessed the potential transfer of the yeast mycosporine myc-glu-glu from the diet into the copepod boeckella antiqua and the ciliate paramecium bursaria ...200616395424
heterologous expression of the benzoate para-hydroxylase encoding gene (cyp53b1) from rhodotorula minuta by yarrowia lipolytica.there is currently an increasing number of cytochrome p450 (cyp450) monooxygenase encoding genes becoming available from various genome-sequencing projects. these enzymes require association with cytochrome p450 reductase (cpr) to achieve optimal activities. in this study, the cyp53b1 gene, which encodes a benzoate para-hydroxylase, was successfully cloned from rhodotorula minuta and overexpressed in yarrowia lipolytica e150. multiple copies of the cyp53b1 cdna were cloned under the pox2 promote ...200616402170
multifocal skeletal tuberculosis with rhodotorula minuta co-infection.multifocal skeletal tuberculosis comprises lesions occurring at 2 or more locations of the skeleton. we present an atypical case of multifocal skeletal tuberculosis coexisting with rhodotorula minuta infection in a non-immunocompromized host. the advantages of computed tomography-guided biopsy in establishing the diagnosis and thus influencing treatment are also discussed.200616718937
[micromorphological features of pleurotus pulmonarius (fr.) quel. and p. ostreatus (jacq.) p. kumm. strains in pure and binary culture with yeasts].micromorphology of oyster mushrooms pleurotus ostreatus (jacq.) quel. and p. pulmonarius (fr.) quel. was studied in pure and binary culture with yeasts (cryptococcus laurentii 1629, rhodotorula minuta 2790, sporidiobolus salmonicolor (see text for symbol) 31a-11, candida krusie 3452, pichia holstii 3438). the cultures were cultivated on malt-agar and water agar. various mycelial structures were described: strands, rings, thin searching mycelium, clamps, crystals, head-like offshoots, mycelial fr ...200616737183
rhodotorula benthica sp. nov. and rhodotorula calyptogenae sp. nov., novel yeast species from animals collected from the deep-sea floor, and rhodotorula lysiniphila sp. nov., which is related phylogenetically.three novel species of the genus rhodotorula are described. rhodotorula benthica sp. nov. (type strain jcm 10901(t) = sy-91(t)) and rhodotorula calyptogenae sp. nov. (type strain jcm 10899(t) = sy-86(t)) were respectively isolated from the tubeworm lamellibrachia sp. and the giant white clam calyptogena sp., collected from the deep-sea floor of the pacific ocean off japan. rhodotorula lysiniphila sp. nov. (type strain jcm 5951(t)) is proposed for strains isolated previously in japan and pakistan ...200312807219
stability of microbial communities in goat milk during a lactation year: molecular approaches.the microbial communities in milks from one herd were evaluated during 1-year of lactation, using molecular methods to evaluate their stability and the effect of breeding conditions on their composition. the diversity of microbial communities was measured using two approaches: molecular identification by 16s and 18s rdna sequencing of isolates from counting media (two milks), and direct identification using 16s rdna from clone libraries (six milks). the stability of these communities was evaluat ...200717604934
molecular fingerprinting by pcr-denaturing gradient gel electrophoresis reveals differences in the levels of microbial diversity for musty-earthy tainted corks.the microbial community structure of cork with marked musty-earthy aromas was analyzed using denaturing gradient gel electrophoresis of amplified ribosomal dna. cork stoppers and discs were used for dna extraction and were analyzed by using selective primers for bacteria and fungi. stoppers clearly differed from discs harboring a different fungal community. moreover, musty-earthy samples of both types were shown to have a specific microbiota. the fungi penicillium glabrum and neurospora spp. wer ...200919201983
direct spectroscopic (ftir) detection of intraspecific binary contaminations in yeast cultures.fourier transform infrared spectroscopy has proved to be a good method to identify and characterize microorganisms. this technique has been proposed as a tool to determine the level of contamination in binary mixtures of strains belonging to different species and even to diverse kingdoms, showing a good linear relationship between spectral outputs and contamination levels. the monitoring of intraspecific contamination is a critical point in both laboratory practice and industrial monitoring, but ...200919281476
biosynthesis of polyhydroxyalkanotes in wild type yeasts.biosynthesis of biodegradable polymers polyhydroxyalkanotes (phas) have been studied extensively in wild type and genetically modified prokaryotic cells, however the content and structure of phas in wild type yeasts is not well documented. the purpose of this study was to screen yeast isolates collected from different ecosystems for their ability to accumulate phas. identification of the isolates and characterization of phas produced by the positive isolates were investigated. one positive isola ...200919469284
[risk factor for lifestyle and way of living for symptoms of sick building syndrome: epidemiological survey in japan].to investigate the association among symptoms of sick building syndrome (sbs). self-reported questionnaire and indoor environmental surveys of newly build dwellings in japan were conducted.200919502765
pilot-scale production and liquid formulation of rhodotorula minuta, a potential biocontrol agent of mango anthracnose.to develop a pilot-plant fermentation process for the production of the yeast rhodotorula minuta, to be used as a biocontrol agent of mango anthracnose, using a low-cost culture medium. to develop a stable liquid formulation that preserve high viability of the yeast stored at 4 degrees c.200516108795
development of a serum-free system for the in vitro cultivation of brugia malayi infective-stage larvae.over the past several years, numerous attempts have been made to culture the infective-stage (l3) larvae of the human filarial parasite brugia malayi in an in vitro system that promotes molting to the fourth larval stage (l4). although there have been reports in the literature of successful l3 to l4 development in vitro, all of these systems have required serum supplementation. the complexity of serum as a culture supplement has made reproducibility of results and identification of specific fact ...200011038308
use of rhodotorula minuta live cells hosted in water-in-oil macroemulsion for biotrasformation reaction.a lecithin/water/isooctane water-in-oil (w/o) macroemulsion was used as a host system for biotransformation reactions. in particular, the hydrolytic activity of the yeast rhodotorula minuta toward (+/-)-succinic acid bis-2-isopropyl-5-methylcyclohexyl ester and p-nitrophenyl butyrate is reported. evidence that r. minuta entrapped in w/o macroemulsion is able to hydrolyze the p-nitrophenyl butyrate ester is presented. by performing the yeast-catalyzed hydrolysis of (+/-)-succinic acid bis-2-isopr ...200616739950
two yeast species cystobasidium psychroaquaticum f.a. sp. nov. and cystobasidium rietchieii f.a. sp. nov. isolated from natural environments, and the transfer of rhodotorula minuta clade members to the genus cystobasidium.many species of dimorphic basidiomycetes are known only in their asexual phase and typically those pigmented in different hues of red have been classified in the large polyphyletic genus rhodotorula. these yeasts are ubiquitous and include a few species of some clinical relevance. the phylogenetic distribution of rhodotorula spans three classes: microbotryomycetes, cystobasidiomycetes and exobasidiomycetes. here, the presented multi-gene analyses resolved phylogenetic relationships between the s ...201525367339
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