[on the similarity of antigen between torulopsis and salmonella (author's transl)].the immune sera for torulopsis apicola, t. bovine, t. colliculosa, t. fructus, t. glabrata, t. haemulonii and t. kestoni agglutinated salmonella cholerae-suis (6,7). the immune serum for s. cholerae-suis agglutinated torulopsis apicola, t. bovina, t. colliculosa, t. fructus, t. glabrata, t. haemulonii and t. kestoni. absorption and agglutination cross test demonstrated common antigen factors in the tested torulopsis species and in s. cholerae-suis (6,7).197770908
cytochromes p450 of the sophorose lipid-producing yeast candida apicola: heterogeneity and polymerase chain reaction-mediated cloning of two genes.candida apicola belongs to a group of yeasts producing high amounts of surface-active extracellular glycolipids consisting of sophorose and long-chain-omega- and (omega-1)-hydroxy fatty acids. the involvement of cytochrome p450 in the synthesis of sophorose lipid by the hydroxylation of long-chain fatty acids was suggested from a simultaneous increase of cellular p450 content. hydroxylation studies indicated the existence of multiple p450 forms capable of hydroxylating not only long-chain fatty ...19968771711
yeast communities associated with stingless bees.the yeast communities associated with the stingless bees tetragonisca angustula, melipona quadrifasciata and frieseomelitta varia were studied. the bees t. angustula and f. varia showed a strong association with the yeast starmerella meliponinorum. m. quadrifasciata more frequently carried a species related to candida apicola, but also vectored low numbers of s. meliponinorum. some of the yeasts isolated from adult bees were typical of species known to occur in flowers. other yeast species found ...200314654431
application of oil refinery waste in the biosynthesis of glycolipids by yeast.candida antarctica or candida apicola synthesized surfactants (glycolipids) in the cultivation medium supplemented with oil refinery waste, either with soapstock (from 5.0% to 12.0% v/v) or post-refinery fatty acids (from 2.0% to 5.0% v/v). the efficiency of glycolipids synthesis was determined by the amount of waste supplemented to the medium and was from 7.3 to 13.4 g/l and from 6.6 to 10.5 g/l in the medium supplemented with soapstock and post-refinery fatty acids, respectively. the studied y ...200415207288
quality of silages from italian farms as attested by number and identity of microbial indicators.this study evaluated the quality and possible hygiene risks related to farm-made silages by analysing the presence and number of micro-organisms that influence the preservation and safety in samples from four italian regions.200717953581
rapd analysis of salt-tolerant yeasts from contaminated seasoned pickled plums and their growth inhibition using food additives.eight salt-tolerant yeasts were isolated from contaminated pickled plums which were seasoned with honey and "umami" seasoning. they were classified into four main groups according to random amplified polymorphic dna analysis, and three of ten kinds of food additives tested inhibited their growth. the type strains of each group were identified as zygosaccharomyces bisporus, pichia subpeliculosa, and two strains of candida apicola based on the d1/d2 region sequence of the 26s rrna gene. they were ...200819127651
molecular identification and osmotolerant profile of wine yeasts that ferment a high sugar grape must.the objective of this study was to examine the saccharomyces and non-saccharomyces yeast populations involved in a spontaneous fermentation of a traditional high sugar must (vino cotto) produced in central italy. molecular identification of a total of 78 isolates was achieved by a combination of pcr-rflp of the 5.8s its rrna region and sequencing of the d1/d2 domain of the 26s rrna gene. in addition, the isolates were differentiated by rapd-pcr. only a restricted number of osmotolerant yeast spe ...200919230999
directed microbial biosynthesis of deuterated biosurfactants and potential future application to other bioactive molecules.deuterated rhamnolipids were produced using strain ad7 of pseudomonas aeruginosa, which was progressively adapted to increasing levels of deuterium in d(2)o and carbon substrates. fourteen different deuterated rhamnolipid structures, including structural isomers, were produced which is similar to normal protonated structures. there were two main products monorhamnolipid rha-c(10)-c(10) and dirhamnolipid rha(2)-c(10)-c(10). the levels of deuteration varied from 16% with 25% d(2)o + h-glycerol to ...201020405122
production of sophorolipid biosurfactants by multiple species of the starmerella (candida) bombicola yeast clade.sophorolipids are carbohydrate-based, amphiphilic biosurfactants that are of increasing interest for use in environmentally benign cleaning agents. sophorolipid production was tested for 26 strains representing 19 species of the starmerella yeast clade, including starmerella bombicola and candida apicola, which were previously reported to produce sophorolipids. five of the 19 species tested showed significant production of sophorolipids: s. bombicola, c. apicola, candida riodocensis, candida ste ...201020738402
cytochrome p450 reductase from candida apicola: versatile redox partner for bacterial p450s.candida apicola belongs to a group of yeasts producing surface-active glycolipids consisting of sophorose and long-chain (ω)- or (ω-1)-hydroxy fatty acids. hydroxylation of the fatty acids in this strain is likely catalyzed by cytochrome p450 monooxygenases (p450), which require reducing equivalents delivered via a cytochrome p450-diflavin reductase (cpr). we herein report cloning and characterization of the cpr gene from c. apicola atcc 96134. the gene encoding a protein of 687 amino acids was ...201322526787
fructanase and fructosyltransferase activity of non-saccharomyces yeasts isolated from fermenting musts of mezcal.fructanase and fructosyltransferase are interesting for the tequila process and prebiotics production (functional food industry). in this study, one hundred thirty non-saccharomyces yeasts isolated from "mezcal de oaxaca" were screened for fructanase and fructosyltransferase activity. on solid medium, fifty isolates grew on agave tequilana fructans (atf), inulin or levan. in liquid media, inulin and atf induced fructanase activities of between 0.02 and 0.27u/ml depending of yeast isolate. high f ...201222336744
high-quality draft genome sequence of candida apicola nrrl y-50540.candida apicola, a highly osmotolerant ascomycetes yeast, produces sophorolipids (biosurfactants), membrane fatty acids, and enzymes of biotechnological interest. the genome obtained has a high-quality draft for this species and can be used as a reference to perform further analyses, such as differential gene expression in yeast from candida genera.201526067948
starmerella neotropicalis f. a., sp. nov., a yeast species found in bees and pollen.a novel yeast species was found repeatedly and in high cell densities in underground-nesting stingless bees of the species melipona quinquefasciata and their provisions in northern minas gerais (brazil). one additional strain was isolated from bee-collected pollen in cuba. phylogenetic analyses based on rrna gene sequences (d1/d2 large subunit gene and internal transcribed spacer) indicated that the novel species belongs to the starmerella clade and is most closely related to candida (iter. nom. ...201323959828
identification and partial characterization of extracellular aspartic protease genes from metschnikowia pulcherrima iwbt y1123 and candida apicola iwbt y1384.the extracellular acid proteases of non-saccharomyces wine yeasts may fulfill a number of roles in winemaking, which include increasing the available nitrogen sources for the growth of fermentative microbes, affecting the aroma profile of the wine, and potentially reducing protein haze formation. these proteases, however, remain poorly characterized, especially at genetic level. in this study, two extracellular aspartic protease-encoding genes were identified and sequenced, from two yeast specie ...201222820332
candida kuoi sp. nov., an anamorphic species of the starmerella yeast clade that synthesizes sophorolipids.a novel strain of anamorphic yeast, designated strain nrrl y-27208(t), was isolated from concentrated grape juice in cape province, south africa. analysis of nuclear large subunit rrna gene sequences from the d1/d2 domains separated the novel isolate from strains of starmerella bombicola and starmerella meliponinorum, as well as from species of the genus candida that are members of the starmerella clade. compared to previously described species, strain nrrl y-27208(t) is most closely related to ...201222268073
kinetics of enzymatic lysis, formation and regeneration of protoplasts ofcandida (torulopsis) apicola.the optimal conditions for protoplast formation ofcandida apicola were by using an enzyme fromarthrobacter sp. in combination with 2-mercaptoethanol. the kinetic data support the two-layered structure model of cell wall for this yeast but the structure of the cell wall depended on the age of cells and culture conditions. to regenerate the protoplasts, the type of osmotic stabilizer was important: sorbitol gave 16 to 30% regeneration. electron microscopy revealed the presence of vesicles in the s ...199224425327
the use of parsimony network analysis for the formal delineation of phylogenetic species of yeasts: candida apicola, candida azyma, and candida parazyma sp. nov., cosmopolitan yeasts associated with floricolous insects.parsimony network analysis of rdna sequences was used to delimit phylogenetic species of yeasts in an objective, formal manner. many strains assigned to candida apicola (starmerella clade), when compared to the type, fell outside the inclusion limits proposed by kurtzman and robnett (1998) based on a pair-wise comparison of the large subunit rrna gene d1/d2 domains. however, when these sequences were analyzed jointly with its rdna sequences by parsimony network analysis, 28 of the 30 strains for ...201019941164
correction for vega-alvarado et al., high-quality draft genome sequence of candida apicola nrrl y-50540. 201526316641
petite mutants of sophorolipid-producing candida yeasts.treatment of both candida apicola imet 43747 and candida bombicola atcc 22214 with n-methyl-n'-nitro-n-nitroso guanidine resulted in formation of auxotrophic mutants and cytochrome oxidase negative mutants. the deficiency of intact cytochrome oxidase did not affect the ability to produce sophorose lipid. this indicates that intact mitochondrial energy supplying system is not necessary for the production of the extracellular sophorose lipids by both candida yeasts.19948207665
elucidation of the structure of an unusual cyclic glycolipid from torulopsis apicola. 19902081340
torulopsis apicola cytopathic effects on renal tissue cultures of primates. 20037197907
torulopsis apicola nov. spec., new isolated from bees. 195813595625
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