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[anti-infective phytotherapies of the tree-savannah, senegal (occidental africa). iii: a review of phytochemical substances and the antimicrobial activity of 43 species].a review has been made of the ethnobotanical and pharmacological data of 43 medicinal plants of the tree-savannah used by the diola against infectious diseases. the traditional use of ten plants can be explained by pharmacologically active principles: adansonia digitata, azadirachta indica, carica papaya, cassia tora, fagara leprieurii, guiera senegalensis, khaya senegalensis, mangifera indica, psidium guajava and voacanga africana. four of these herbs are recommended for use in primary health c ...19892664354
folklore therapeutic indigenous plants in periodontal disorders in india (review, experimental and clinical approach).though a number of plants and their parts are used for dental ailments among population in rural and urban areas of developing countries, in india however, the most common house-hold, road-side plants are mango (mangifera indica), neem (azadirachta indica; melia azadirachta), ocimum (ocimum basilicum), tea-dust (camellia sinensis) and uncommonly murayya, i.e., currey leaf (murayya koenigi) [chopra et al. 1958, kirtikar and basu 1935, nadakarni 1954, satyavati 1984]. the leaves of these plants ar ...19883042642
lectin agglutination of thermophilic campylobacter species.agglutination tests with lectins indicated differences in the surface composition of strains of the thermophilic (optimum temperature 42 degrees c) campylobacter species c. coli, c. faecalis, c. hyointestinalis, c. jejuni and c. laridis. all strains examined were agglutinated by the protein-reactive agglutinins of mangifera indica (mango) and persea americana (avocado) and a large proportion was also agglutinated by the carbohydrate-reactive lectins of canavalia ensiformis (jack bean) and tritic ...19873439012
[biochemical aspects of resistance mechamism of mango (mangifera indica l. to ceratocystis fimbriata ell. & hals]. 19744461018
differentiation of coagulase-positive and coagulase-negative staphylococci by lectins and plant agglutinins.the screening of staphylococci with a panel of 14 lectins and extracts demonstrating lectin-like activity led to the development of a rapid agglutination slide test for the differentiation of certain coagulase-negative staphylococci and human strains of staphylococcus aureus. the coagulase-negative staphylococci were agglutinated by agglutinins from mangifera indica, triticum vulgaris, and crude limulus polyphemus. the test is rapid, requiring only 5 to 15 min to identify an unknown strain of st ...19827068834
[in vitro effect against giardia of 14 plant extracts].to investigate antigiardiasic activity in plants used in mexico as antidiarrheics and/or antiparasitics.19947839013
[antiviral activity of mangiferin against herpes simplex virus type 2 in vitro].the effect of mangiferin, a tetrahydroxy pyrrolidone saponin extracted from the leaves of mango (mangifera indica), against herpes simplex virus type 2 (hsv-2) in vitro was studied. the 50% effective concentration (ec50) of it against hsv-2 plaque formation in hela cells was 111.7 micrograms.ml-1, and the concentrations of 33 and 80 micrograms.ml-1 reduced the virus replicative yields by 90% (ec90) and 99% (ec99), respectively. the therapeutic index (ic50/ec50) was 8.1. mangiferin did not direct ...19938010041
new saponins from mangifera indica.two new terpenoidal saponins, indicoside a [1] and indicoside b [2], were isolated from mangifera indica. their structures were determined as 28-hydroxylupa-12,20(29)-diene-3-o-[beta-glucopyranosyl-(1-->2)] [-beta-glucopyranosyl-(1-->3)]-alpha-l-arabinopyranoside [1] and 28-hydroxylupa-12,20(29)-diene-3-o-[beta-glucopyranosyl-(1-->3)-al pha-l-rhamnopyranosyl-(1-->2)][beta-glucopyranosyl(1-->3)]-alpha-l- arabinopyranoside [2] on the basis of chemical and spectroscopic studies.19938326325
[evaluation of the usefulness of the agglutination test with mangifera indica extract for the identification of pathogenic yersinia enterocolitica strains].the study was performed on 137 y. enterocolitica strains belonging to various serological groups, including 75 03 group strains isolated form human clinical material. the agglutination test on slides was carried out on this strains using mangifera indica extract of own production. agglutinating preparation obtained from the seeds of m. indica agglutinated y. enterocolitica organisms possessing the pvy plasmid and crmox+ phenotype in dilutions to 1.56 micrograms/ml. in identification tests conduc ...19979554150
in vitro evaluation of medicinal plant extracts against pestalotiopsis mangiferae.a serious leaf-spot disease of mangifera indica was noted during the last 10 years in satpura plateau of india. on the basis of characteristic symptoms and cultural characters, the pathogen was identified as pestalotiopsis mangiferae which is hitherto not reported from satpura plateau of india. screening of 17-medicinal plants against the test pathogen revealed 14 antimycotic whereas 3-plants, viz., argemone mexicana, caesalpinia bonducella, and casia fistula acclerated the growth of the pathoge ...19969676046
antiamoebic and phytochemical screening of some congolese medicinal plants.results from the in vitro antiamoebic activity of some congolese plant extracts used as antidiarrhoeic in traditional medicine indicated that of 45 plant extracts tested, 35 (77.78%) exhibited an antiamoebic activity and 10 (22.22%) were inactive. the highest activity (mic < 100 microg/ml) was obtained with extracts from root bark of paropsia brazzeana, cryptolepis sanguinolenta, alchornea cordifolia, hensia pulchella, maprounea africana, rauwolfia obscura and voacanga africana, leaves and stem ...19989687082
microbial stability of mango (mangifera indica l.) juice preserved by combined application of mild heat and extracts of two tropical spices.the microbial stability of mango (mangifera indica l.) juice (ph 4.9) supplemented with extracts of ginger (zingiber officinale) and nutmeg (myristica fragrans) was investigated during 3 months of ambient-temperature storage. heating at 55 degrees c for 15 min markedly reduced the levels of non-spore-forming bacteria and produced a product with acceptable taste. supplementing mango juice with an agueous extract of ginger (15%, vol/vol) or nutmeg (20%, vol/vol) inhibited the growth of challenge m ...19989709257
plants from puerto rico with anti-mycobacterium tuberculosis properties.this study assesses the antitubercular potential of natural products obtained from plants reputed to have medicinal properties and collected from the tropical flora of puerto rico.19989883470
metabolic effects of sulfur dioxide fumigation on mangifera indica plants. 199910594152
anti-herpes simplex virus activities of crude water extracts of thai medicinal plants.a number of thai medicinal plants, recommended as remedies for herpesvirus infection and have been used in primary health care were investigated for their intracellular activities against herpes simplex viruses (hsv). centella asiatica l., maclura cochinchinensis cornor, and mangifera indica l. contained both anti-hsv-1 and -2 activities, as determined by plaque inhibition assay. an inhibition of the production of infectious hsv-2 virions from infected vero cells could also be demonstrated. comb ...200010715843
antiamoebic and spasmolytic activities of extracts from some antidiarrhoeal traditional preparations used in kinshasa, congo.three major extracts from some traditional preparations, based on medicinal plants, used as antidiarrhoeal agents were investigated for their putative antiamoebic and spasmolytic activities in vitro. results indicated that both biological activities are concentrated in the polyphenolic fraction, and not in the saponin or alkaloid containing fractions. the most active polyphenolic extracts were those from euphorbia hirta whole plant, leaves of alchornea cordifolia, crossopteryx febrifuga, nauclea ...200010782488
analgesic and anti-inflammatory effects of mangifera indica l. extract (vimang).vimang is an aqueous extract of mangifera indica used in cuba to improve the quality of life in patients suffering from elevated stress. to assess its possible analgesic and antiinflammatory effects, the results of a standard extract evaluation are presented. analgesia was determined using acetic acid-induced abdominal constriction and formalin-induced licking. antiinflammatory effects were evaluated using carrageenan- and formalin-induced oedema. vimang (50-1000 mg/kg, p.o.) exhibited a potent ...200111180516
immunomodulatory activity of alcoholic extract of mangifera indica l. in mice.mangifera indica linn, a plant widely used in the traditional medicinal systems of india, has been reported to possess antiviral, antibacterial and anti-inflammatory activities. in the present study, the alcoholic extract of stem bark of mangifera indica linn (extract i containing mangiferin 2.6%), has been investigated for its effect on cell mediated and humoral components of the immune system in mice. administration of test extract i produced increase in humoral antibody (ha) titre and delayed ...200111694357
flavonoids from emblica officinalis and mangifera indica-effectiveness for dyslipidemia.flavonoids from emblica officinalis and mangifera indica effectively reduce lipid levels in serum and tissues of rats induced hyperlipidemia. hepatic hmg coa reductase activity was significantly inhibited in rats fed e. officinalis flavonoids. but increase of this enzyme was observed in rats administered m. indica flavonoids. lcat showed elevated levels in rats fed flavonoids from e. officinalis and m. indica. the degradation and elimination of cholesterol was highly enhanced in both the groups. ...200211744299
effect of mangifera indica l. extract (qf808) on protein and hepatic microsome peroxidation.the antioxidant activities of qf808, a steam bark extract of mangifera indica l., were studied on hydroxyl-mediated oxidation of bovine serum albumin (bsa) and in a hepatic microsome system. the extract was effective in reducing the oxidation of bsa, since its half- maximal inhibition concentration (ic(50)) was 0.0049% w/v in the inhibition of carbonyl group formation and lower than 0.0025% w/v in the inhibition of sulfhydryl group loss. qf808 inhibited lipid peroxidation which was initiated enz ...200111746837
mango seed weevil (coleoptera: curculionidae) and premature fruit drop in mangoes.the effect of infestations of mango seed weevil, sternochetus mangiferae (f.), on premature fruit drop of mangoes was investigated. mango fruits ('haden') of equal size were collected both off the ground and from the tree at four times during the season (june-august). if weevil-infested fruit were more prone to dropping than uninfested fruit, the prediction was that a higher infestation rate would be found in fruit on the ground compared with fruit on the tree. average fruit weight was used as a ...200212020010
white-faced monkey (cebus capucinus) ecology and management in neotropical agricultural landscapes during the dry season.habitat use by a c. capucinus troop was studied in an agricultural landscape during late dry season (march-april 1994) in northwest costa rica. riparian forests, palm canals and living fence rows accounted for 82% of observations, significantly more than the other six habitats present. the study troop consumed 24 species of plants and five animals. feeding concentrated on the introduced african oil palm (elaeis guineensis) (33.6%) and mango (mangifera indica) (27.2%), found mostly in palm canals ...200112189802
anti-salmonella activity of medicinal plants from cameroon.to evaluate the effects of herbal extracts derived from plants commonly prescribed by traditional practitioners for the treatment of typhoid fever.200112201022
the enhancement of the nutritive value of mango seed kernels for poultry by thermal treatment and radiation processing.raw seed kernels of local mango varieties (magnifera indica l.) were analyzed for composition, levels of trypsin inhibitors, tannins, cyanogenetic glucosides, in vitro protein digestibility and apparent metabolizable energy (amen) as being effected by boiling, autoclaving as well as irradiation at 5, 10, 15, and 20 kgy. the air-dry mango seed kernels (msk) contained cp 70 g kg-1, ee 128 g kg-1, and tannins 67 g kg-1. compared with raw kernels the contents of trypsin inhibitory activity (30 tiu g ...200111851017
isolation and quantitative analysis of phenolic antioxidants, free sugars, and polyols from mango (mangifera indica l.) stem bark aqueous decoction used in cuba as a nutritional supplement.an aqueous decoction of mango (mangifera indica l.) stem bark has been developed in cuba on an industrial scale to be used as a nutritional supplement, cosmetic, and phytomedicine. previously we reported its antioxidant activity, and we concluded that the product could be useful to prevent the production of reactive oxygen species and oxidative tissue damage in vivo. a phytochemical investigation of mango stem bark extract has led to the isolation of seven phenolic constituents: gallic acid, 3,4 ...200211829642
the effect of mangifera indica stem bark and pluchea ovalis roots on tracheal smooth muscle in vitro.aqueous extract of the stem bark of mangifera indica and ethanolic extract of the roots of pluchea ovalis has been studied on rat tracheal smooth muscle in vitro. the extract of m. indica at 1, 2 and 4 mg/ml and that of p. ovalis at 0.25, 0.5 and 1 mg/ml relaxed, dose-dependently, the rat tracheal smooth muscle strip previously contracted by acetylcholine at 0.055 mmol/l.200212490220
alpha-glucosidase inhibitory activity of mangifera indica bark.the ethanolic extracts of lawsonia inermis leaves, holarrhena antidysenterica bark, swertia chirata whole plant and mangifera indica bark were tested (in-vitro) for alpha-glucosidase inhibitory activity. m. indica extract was found to be the most potent, with an ic(50) value of 314 microg/ml.200111543970
evaluation of anti-diarrhoeal activity in seed extracts of mangifera indica.mangifera indica is commonly grown in many parts of the world. its seeds have been used for anti-diarrhoeal activity in indian traditional medicine. this study evaluates the potential anti-diarrhoeal activity of methanolic (mmi) and aqueous (ami) extracts of seeds of m. indica in experimental diarrhoea, induced by castor oil and magnesium sulphate in mice. both mmi and ami were given orally in the dose of 250 mg/kg, showed significant anti-diarrhoeal activity comparable with that of the standard ...200312499070
cultural categorization of febrile illnesses in correlation with herbal remedies used for treatment in southwestern nigeria.the ethnographic study was conducted in two communities in oyo state in southwestern nigeria. the study sites consisted of a rural and an urban local government area located in the tropical rain forest zone of nigeria. the study was designed to obtain information on febrile illnesses and herbal remedies for treatment with the aim of identifying potential antimalarial drugs. the study revealed that fever is a general term for describing illnesses associated with elevated body temperature. the ind ...200312639738
lectins in fruits having gastrointestinal activity: their participation in the hemagglutinating property of escherichia coli o157:h7.in fruits with therapeutic properties for antidiarrheal and laxative uses, the presence of lectins may be the bioactive properties that interfere with bacterial adhesion, thought to be competition for glycoside signal sites in the attachment.200111440778
evaluation of antibacterial activity of mangifera indica on anaerobic dental microglora based on in vivo studies.the present study was carried out to compare the subgingival plaque microflora in mango leaf users. fifty subjects of both sexes, 25 of them used tooth brush and 25 used mango leaf as their home care hygiene device were included in the study. the microbiological evaluation for specific bacterial counts of actinobacillus actinomycetemcomitans, prevotella intermedia, porphyromonas gingivalis, fusobacterium nucleatum and peptostreptococcus micros were carried out for all subject. specific microbial ...200212785172
characterization of cross-reacting allergens in mango fruit.allergic reactions to mango fruit have become increasingly important. a cross-reaction between mango fruit, various other foods, and respiratory allergens has been assumed but not investigated until now.200111251404
effect of certain plant extracts on alpha-amylase activity.ethanolic extracts of punica granatum, mangifera indica, boerhaavia diffusa, embelia ribes, phyllanthus maderaspatensis, and withania somnifera, were tested for their effect on alpha-amylase activity (in vitro). p. granatum and m. indica were found to exhibit interesting alpha-amylase inhibitory activity.200111223231
native and introduced host plants of anastrepha fraterculus and ceratitis capitata (diptera: tephritidae) in northwestern argentina.wild or commercially grown, native and exotic fruit were collected in 30 localities in the tucumán province (nw argentina) from january 1990 to december 1995 to determine their status as hosts of anastrepha fraterculus (wiedemann) and/or ceratitis capitata (wiedemann), the only two fruit fly species of economic and quarantine importance in argentina. a total of 84,094 fruit (3,466.1 kg) representing 33 species (7 native and 26 exotic) in 15 plant families were sampled. we determined the followin ...200314503581
[evaluation of the usefulness of selected virulence markers for the identification of virulent yersinia enterocolitica strains. i. phenotypic markders associated with plasmid pyv].the species yersinia enterocolitica includes either pathogenic or non-pathogenic strains. therefore it is necessary to differentiate virulent bacilli from other. it is well known that pathogenic strains of y. enterocolitica bearing virulence associated plasmid called pyv, which could be demonstrated by its isolation or detected by the presence of specific, phenotypic properties directly related with this plasmid. the aim of the presented paper was to check the ability of some phenotypic virulenc ...200011107777
anthelminthic and antiallergic activities of mangifera indica l. stem bark components vimang and mangiferin.this study investigated the antiallergic and anthelmintic properties of vimang (an aqueous extract of mangifera indica family stem bark) and mangiferin (the major polyphenol present in vimang) administered orally to mice experimentally infected with the nematode, trichinella spiralis. treatment with vimang or mangiferin (500 or 50 mg per kg body weight per day, respectively) throughout the parasite life cycle led to a significant decline in the number of parasite larvae encysted in the musculatu ...200314669257
rot of mangifera indica linn. caused by aspergillus. 195114822551
effect of mango weevil (coleoptera: curculionidae) damage on mango seed viability in hawaii.the mango weevil, cryptorhynchus (= sternochetus) mangiferae (f.), is a federally quarantined pest that prevents shipment of mangos from hawaii into the continental united states. although this monophagous weevil allegedly causes reduced seed germination, damage to the fruit pulp, and premature fruit drop in mangos, there are few studies examining these potential sources of crop loss. we conducted studies to assess the effect of mango weevil infestation on seed viability while making observation ...200010985036
phenotypic diversity of xanthomonas sp. mangiferaeindicae.carbohydrate utilization profiles by means of the api (appareils et procédés d'identification) system and sensitivity to antibiotics and heavy metal salts of 68 xanthomonas sp. mangiferaeindicae strains isolated in nine countries from mango (mangifera indica l.) and other genera of the anacardiaceae were examined to assess the variability of the taxon. the strains could be separated into 10 groups according to ward clustering. apigmented strains isolated from the pepper tree [syn. brazilian pepp ...199815244066
evaluation of the in vitro antioxidant activity of mangifera indica l. extract (vimang).an extract of mangifera indica l. (vimang) was tested in vitro for its antioxidant activity using commonly accepted assays. it showed a powerful scavenger activity of hydroxyl radicals and hypochlorous acid and acted as an iron chelator. the extract also showed a significant inhibitory effect on the peroxidation of rat-brain phospholipid and inhibited dna damage by bleomycin or copper-phenanthroline systems.200010960895
isoprene emission from tropical tree species.foliar emission of isoprene was measured in nine commonly growing tree species of delhi, india. dynamic flow enclosure technique was used and gas samples were collected onto tenax-gc/carboseive cartridges, which were then attached to the sample injection system in the gas chromatograph (gc). eluting compounds were analysed using a flame ionisation detector (fid). out of the nine tree species, isoprene emission was found in six species (eucalyptus sp., ficus benghalensis, ficus religiosa, mangife ...200515701397
review on some plants of indian traditional medicine with antioxidant activity.a lot of medicinal plants, traditionally used for thousands of years, are present in a group of herbal preparations of the indian traditional health care system (ayurveda) named rasayana proposed for their interesting antioxidant activities. among the medicinal plants used in ayurvedic rasayana for their therapeutic action, some of these have been throughly investigated. in the present paper seven plants (emblica officinalis l., curcuma longa l., mangifera indica l., momordica charantia l., sant ...200010904144
mangifera indica and phaseolus vulgaris in the bioindication of air pollution in bahia, brazil.in this article are reported the results of a study on the concentration of ascorbic acid (aa) in mangifera indica as passive monitor and in phaseolus vulgaris as active monitor with the intention to study the effects of industrial emissions from the petrochemical complex of camaçari (pcc), bahia, brazil, on the vegetation. leaves from m. indica were collected in two sites in the region under direct influence of industrial emissions and in one presumed nonpolluted reference (background) site. po ...200010903824
antihyperglycaemic effect of mangifera indica in rat.the leaves of mangifera indica are used as an antidiabetic agent in nigerian folk medicine. to determine whether or not there is a scientific basis for this use, the effect of the aqueous extract of the leaves on blood glucose level was assessed in normoglycaemic, glucose - induced hyperglycaemic and streptozotocin (stz)-induced diabetic rats. the aqueous extract given orally (1 g/kg) did not alter the blood glucose levels in either normoglycaemic or stz-induced diabetic rats. in glucose - induc ...199910479762
reversed-phase gradient high-performance liquid chromatographic procedure for simultaneous analysis of very polar to nonpolar retinoids, carotenoids and tocopherols in animal and plant samples.a reversed-phase gradient high-performance liquid chromatographic (hplc) procedure, which utilizes gradient elution and detection by a photodiode-array detector, has been developed to analyze simultaneously very polar retinoids, such as 4-oxo-retinoyl-beta-glucuronide, retinoyl beta-glucuronide and 4-oxo-retinoic acid; polar retinoids, such as retinoic acid and retinol; nonpolar retinoids, such as retinyl esters; along with xanthophylls, monohydroxy carotenoids, hydrocarbon carotenoids, and toco ...19989613935
protein kinase activities in ripening mango, mangifera indica l., fruit tissue. i: purification and characterization of a calcium-stimulated casein kinase-i.a ca(2+)-stimulated protein kinase (pk-i), active with dephosphorylated casein as exogenous substrate, was purified from ripening mango fruit. the purification procedure involved 30-70% ammonium sulphate fractionation and sequential anion exchange-, affinity-, hydrophobic interaction- and gel filtration chromatography. pk-i was purified ca. 40-fold with an overall yield of < 1%. the final specific activity in the presence of 0.1 mm ca2+ was 55 nmol min-1 mg-1. analysis of the most highly purifie ...19989507068
[microbiological quality of street sold fruits in san josé, costa rica].the sanitary quality of street sold fruits was analyzed during the period from march 1990 thru march 1993 in san jose, costa rica. it looked for the presence of salmonella spp. shigella spp., escherichia coli as well as fecal coliforms in natural refreshments, fruit salads and the fruits most frecuently expended on streets, either in slices as the pineapple (ananas comosus), papaya (carica papaya), non-ripe mangoe (mangifera indica) and watermelon (citrullus vulgaris) and those that can be eaten ...19958729262
alternative oxidase from mango (mangifera indica, l.) is differentially regulated during fruit ripening.alternative oxidase is a respiratory-chain component of higher plants and fungi that catalyzes cyanide-resistant oxygen consumption. the activity of a alternative oxidase has been detected during ripening in several climacteric fruit including mango (mangifera indica l.). synthetic oligonucleotides, corresponding to conserved regions of the sauromatum guttatum and arabidopsis thaliana nucleotide sequences, were used as primers for polymerase chain reaction to amplify genomic dna extracted from m ...19958555961
carotenoid-protein complexes.this chapter provides an updated report of carotenoprotein-related research. very few carotenoproteins have been purified; however, their presence in aqueous extracts may be indicated by spectroscopic evidence. carotenoproteins have been isolated, purified, and characterized from the ectoderm, exoskeleton, eggs, and ovaries of marine invertebrates, especially crustaceans. water-soluble and detergent-soluble carotenoid-protein complexes have also been isolated from the cytoplasmic membrane of som ...19938469154
agglutination of pyv+ yersinia enterocolitica strains by agglutinin from mangifera indica.agglutination of 271 strains of yersinia enterocolitica and related species grown at 37 degrees c by a 0.01% dilution of the agglutinin from mangifera indica was correlated with the presence of the virulence plasmid. the study of yada mutants suggested that the yada protein is the target of the plant agglutinin.19957751396
evaluation of ash from some tropical plants of nigeria for the control of sclerotium rolfsii sacc. on wheat (triticum aestivum l.).eleven ash samples, from organs of nine tropical plants, were screened for their abilities to inhibit mycelial growth and sclerotial germination of a nigerian isolate of sclerotium rolfsii on agar and in the soil. ten ash samples showed some activity against mycelial growth of s. rolfsii in vitro. ash samples from delonix regia stem wood, mangifera indica leaf and vernonia amygdalina leaf were most effective as each totally inhibited mycelial growth of s. rolfsii in vitro. ocimum gratissimum lea ...199816284852
peroxisomal thiolase mrna is induced during mango fruit ripening.fruit ripening is a complex, developmentally regulated process. a series of genes have been isolated from various ripening fruits encoding enzymes mainly involved in ethylene and cell wall metabolism. in order to aid our understanding of the molecular basis of this process in a tropical fruit, a cdna library was prepared from ripe mango (mangifera indica l. cv. manila). by differential screening with rna poly(a)+ from unripe and ripe mesocarp a number of cdnas expressing only in ripe fruit have ...19957640354
[scytalidium dimidiatum an opportunistic fungus for both man and mangifera indica trees in venezuela].the purpose of this study was to confirm the presence of scytalidium dimidiatum on mangifera indica (mango) trees, in a plantation managed by a diabetic patient with a white grain mycetoma of the foot caused by the same fungus. samples from necrotic apices, roots, burned leaves and rotten stems from eight trees were processed by the smith and furcolow's mineral oil technique (modified). several isolates from the apex material and clinical samples from the diabetic patient isolated in pure cultur ...200516309356
the interaction of brucella cell surface components with plant agglutinins.cells of all brucella species were agglutinated by the basic protein-reactive lectins of mangifera indica and persea americana. cells of non-smooth strains including b. ovis and b. canis but not smooth strains were also agglutinated by concanavalin a. these lectins also reacted in a similar pattern with lipopolysaccharide extracts of these organisms. the lectin from bandeiraea simplicifolia precipitated with the lipopolysaccharide antigens of smooth brucella organisms and with those of serologic ...19846208071
the effects of fruit juices and fruits on the absorption of iron from a rice meal.the effects of the chemical composition of fruit juices and fruit on the absorption of iron from a rice (oryza sativa) meal were measured in 234 parous indian women, using the erythrocyte utilization of radioactive fe method. the corrected geometric mean fe absorptions with different juices varied between 0.040 and 0.129, with the variation correlating closely with the ascorbic acid contents of the juices (rs 0.838, p less than 0.01). ascorbic acid was not the only organic acid responsible for t ...19873593665
incidence of heat-resistant fungi in nsukka, southern nigeria.a total of 42 soil samples collected from different parts of nsukka, southern nigeria, and 20 freshly plucked mango fruits were screened for the presence of heat-resistant fungi. each soil sample, suspended in sterile water, and washings from each mango fruit were separately heated at 70 degrees c for 1 h before plating on double-strength potato dextrose agar containing chloramphenicol and incubating at approx. 28 degrees c. approximately 98% of all soil samples and 17% of mango fruits contained ...19911909547
the effects of low-dose gamma-irradiation on the wholesomeness of mangoes (mangifera indica) as determined by short-term feeding studies using rats.1. a control diet and diets containing 150 g non-irradiated or 150 g irradiated mangopulp/kg were given to female rats from day 15 of the gestation period until weaning in trials 1 and 2, and from 40 d before mating until 28 d post weaning in trial 3. 2. food intake and dry-matter digestibility were similar with all diets. 3. there were no significant differences between animals given the different dietary regimens in the daily body-weight changes of weanling males, pups, nursing females or fema ...19761244845
tylenchida associated with different crops in sennar state (sudan).a study was done on the taxonomy and morphology of plant parasitic nematodes (tylenchida) found in sennar state (sudan). sixty samples of different crops were collected in the sugarcane area. thirty samples originated from soil around the roots of saccharum officinarum (sugarcane) from different ratoons and thirty samples were collected from other crops (mangifera indica; citrus limon; citrus aurantifolia; citrus paradisi; citrus sinensis, phoenix dactylifera, musa sapentium; cassia italica, cap ...200112425088
modulation of rat macrophage function by the mangifera indica l. extracts vimang and mangiferin.vimang is an aqueous extract of mangiferia indica l., traditionally used in cuba as an anti-inflammatory, analgesic and antioxidant. in the present study, we investigated the effects of vimang and of mangiferin (a c-glucosylxanthone present in the extract) on rat macrophage functions including phagocytic activity and the respiratory burst. both vimang and mangiferin showed inhibitory effects on macrophage activity: (a) intraperitoneal doses of only 50-250 mg/kg markedly reduced the number of mac ...200212095170
tropical fruit trees as bioindicators of industrial air pollution in southeast brazil.psidium guajava l., psidium cattleyanum sabine and mangifera indica l. were tested under field conditions as possible tropical bioindicators of industrial air pollution. the study was performed around the industrial complex of cubatão, se brazil, which comprises 23 industries, including fertilizer, cement, chemical, petrochemical, and steel plants, with 110 production units and 260 emission sources of pollutants. saplings were exposed to environmental conditions during four periods of 16 weeks e ...200212437286
effect of mangiferin on mitochondrial energy production in experimentally induced myocardial infarcted rats.mangiferin, from the leaves of mangifera indica linn., has been suggested as useful in the treatment of cardiovascular disorders. in the present study this drug was examined on the alteration of cardiac energy metabolism in isoproterenol (isph) administered myocardial infarcted rats. isph (20 mg/kg b.w.), which was administered s.c. twice at an interval of 24 h, caused a significant decrease in the activities of tca cycle enzymes and antioxidant defense enzymes with a concomitant increase in the ...200616697266
leaf photosynthesis of 'cat hoa loc' mango (mangifera indica l.) and 'nam roi' pummelo (citrus grandis (l.) osbeck.) in the rainy season.with an ears--plant photosynthesis meter we determined gross photosynthesis (p) of leaves of 'cat hoa loc' mango on 4 years old trees planted in the fruit tree experiment and production station, campus 2, department of crop science, college of agriculture, can tho university in the rainy season of the year 2000. the same experiment was conducted on 'nam roi' pummelo on a farm located in the binh minh district, vinh long province. in the rainy season the calculated gross photosynthesis of 'cat ho ...200212491966
protective effect of mangifera indica l. extract (vimang) on the injury associated with hepatic ischaemia reperfusion.the effect of mangifera indica l. extract (vimang) on treatment of injury associated with hepatic ischaemia/reperfusion was tested. vimang protects from the oxidative damage induced by oxygen-based free radicals as shown in several in vitro test systems conducted. the ability of vimang to reduce liver damage was investigated in rats undergoing right-lobe blood fl ow occlusion for 45 min followed by 45 min of reperfusion. the ischaemia/reperfusion model leads to an increase of transaminase (alt a ...200312672145
soil chemical changes under irrigated mango production in the central são francisco river valley, brazil.irrigated areas in brazil's central são francisco river valley have experienced declines in productivity, which may be a reflection of changes in soil chemical properties due to management. this study was conducted to compare the chemical composition of soil solutions and cation exchange complexes in a five-year-old grove of irrigated mango (mangifera indica l. cv. tommy atkins) with that of an adjacent clearing in the native caatinga vegetation. a detailed physiographic characterization of the ...200312931897
bioindication of air pollution effects near a copper smelter in brazil using mango trees and soil microbiological properties.a field study near the copper smelter of a large industrial complex examined air pollution effects on vegetation and soil parameters in camaçari (northeast brazil). close to the smelter, soil ph-value was lower and total acidity as well as organic carbon contents were higher compared with a site far from the source and two reference sites. the acidification of top soil particularly and the drastically enhanced plant-available copper concentrations were caused by atmospheric deposition. high sulp ...200312963292
mangifera indica l. extract (vimang) and mangiferin modulate mouse humoral immune responses.the present study investigated the effects of orally administered vimang (an aqueous extract of mangifera indica) and mangiferin (the major polyphenol present in vimang) on mouse antibody responses induced by inoculation with spores of microsporidian parasites. inoculation induced specific antibody production with an exponential timecourse, peaking after about one month. vimang significantly inhibited this antibody production from about three weeks post-inoculation, and most markedly by four wee ...200314669253
in vivo and in vitro anti-inflammatory activity of mangifera indica l. extract (vimang).a standard aqueous extract of mangifera indica l., used in cuba as an antioxidant under the brand name of vimang, was tested in vivo for its anti-inflammatory activity using commonly accepted assays. m. indica extract, administered topically (0.5-2 mg per ear), reduced ear edema induced by arachidonic acid (aa) and phorbol myristate acetate (pma, ed50 = 1.1 mg per ear) in mice. in the pma model, m. indica extract also reduced myeloperoxidase (mpo) activity. this extract p.o. administered also in ...200415177302
vascular effects of the mangifera indica l. extract (vimang).the effects of the mangiferia indica l. (vimang) extract, and mangiferin (a c-glucosylxanthone of vimang) on the inducible isoforms of cyclooxygenase (cyclooxygenase-2) and nitric oxide synthase (inos) expression and on vasoconstrictor responses were investigated in vascular smooth muscle cells and mesenteric resistance arteries, respectively, from wistar kyoto (wky) and spontaneously hypertensive (shr) rats. vimang (0.5-0.1 mg/ml) and mangiferin (0.025 mg/ml) inhibited the interleukin-1beta (1 ...200415381052
effect of mangiferin on hyperglycemia and atherogenicity in streptozotocin diabetic rats.in the present study, the effect of mangiferin (a xanthone glucoside, isolated from the leaves of mangifera indica) on the atherogenic potential of streptozotocin (stz)-diabetes was investigated. in addition, the effect of mangiferin on oral glucose tolerance in glucose-loaded normal rats was also determined. the chronic intraperitoneal (i.p.) administration of mangiferin (10 and 20 mg/kg) once daily (o.d.) for 28 days exhibited antidiabetic activity by significantly lowering fasting plasma gluc ...200515740886
antioxidant in mango (mangifera indica l.) pulp.this work was carried out to investigate the pulp composition of four mango cultivars (haden, tommy atkins and ubá) at the ripening stage in relation to three components with antioxidant potential (total phenolics, carotenoids and ascorbic acid). total phenolic compound content was estimated by the folin-ciocalteu reagent and total carotenoid content by spectrophotometry at 450 nm. the contents of beta-carotene and total vitamin c (ascorbic acid and dehydroascorbic acid) were quantified by high ...200717243011
response of laying hens and growing broilers to the dietary inclusion of mango (mangifera indica l.) seed kernel meal.studies were carried out to assess the nutritive value and utilization of mango (mangifera indica l.) seed kernel (msk) in the diets of layer and broiler chickens. the physical component of mango seeds was found to consist of 678 g/kg kernel, 292 g/kg shell and 30 g/kg testa. meal from the seed kernel contained 61.6 g crude protein, 136.2 g ether extract, 22.3 g ash, 46.4 g crude fibre, 673.5 g nitrogen-free extract and appreciable mineral content. in the layers trial, msk replaced maize weight ...200515742869
mangifera indica l. extract (vimang) inhibits fe2+-citrate-induced lipoperoxidation in isolated rat liver mitochondria.the extract of mangifera indica l. (vimang) is able to prevent iron mediated mitochondrial damage by means of oxidation of reduced transition metals required for the production of superoxide and hydroxyl radicals and direct free radical scavenging activity. in this study we report for the first time the iron-complexing ability of vimang as a primary mechanism for protection of rat liver mitochondria against fe2+ -citrate-induced lipoperoxidation. thiobarbituric acid reactive substances (tbars) a ...200515749457
effect of mangiferin on radiation-induced micronucleus formation in cultured human peripheral blood lymphocytes.irradiation causes a variety of lesions in important biomolecules of the cell through generation of free radicals leading to genomic instability. dna strand breaks, acentric fragments, or defective kinetochores are manifested as micronuclei after the first cell division. chemicals that can trap free radicals may reduce the deleterious effects of ionizing radiation. mangiferin (mgn), a glucosylxanthone derived from mangifera indica (mango), was investigated for its ability to reduce the frequency ...200515795888
antibacterial properties of traditionally used indian medicinal plants.in search of broad-spectrum antibacterial activity from traditionally used indian medicinal plants, 66 ethanolic plant extracts were screened against nine different bacteria. of these, 39 extracts demonstrated activity against six or more test bacteria. twelve extracts showing broad-spectrum activity were tested against specific multidrug-resistant (mdr) bacteria, methicillin-resistant staphylococcus aureus (mrsa) and extended spectrum beta-lactamases (esbetal)-producing enteric bacteria. in vit ...200717440624
radioprotection by mangiferin in dbaxc57bl mice: a preliminary study.the radioprotective effects of various concentrations (0, 0.25, 0.5, 1, 2, 5, 10, 17.5, 25, 50, 75 and 100 mg/kg b.wt.) of mangiferin (mgn) was studied in the dbaxc57bl mice whole body exposed to 10 gy of gamma-irradiation. treatment of mice with different doses of mgn, one hour before irradiation reduced the symptoms of radiation sickness and delayed the onset of mortality when compared with the non-drug treated irradiated controls. the radioprotective action of mgn increased in a dose dependen ...200515830843
antioxidant activity of dhc-1, an herbal formulation, in experimentally-induced cardiac and renal damage.dhc-1, an herbal formulation derived from the popular plants bacopa monniera, emblica officinalis, glycyrrhiza glabra, mangifera indica and syzygium aromaticum was studied for its antioxidant activity. the protective effect of dhc-1 in isoproterenol-induced myocardial infarction and cisplatin-induced renal damage were studied. a significant reduction in the serum markers of heart and kidney damage and the extent of lipid peroxidation with a concomitant increase in the enzymatic (sod and cat) and ...200515934019
acetobacter senegalensis sp. nov., a thermotolerant acetic acid bacterium isolated in senegal (sub-saharan africa) from mango fruit (mangifera indica l.).a thermotolerant acetic acid bacterium, designated strain cwbi-b418(t), isolated in senegal from mango fruit (mangifera indica), was characterized in detail by means of genotypic and phenotypic methods. the novel strain was strictly aerobic and exhibited optimal growth on ygm medium at 35 degrees c. cells were gram-negative, motile and coccoid. the strain was assigned to the genus acetobacter on the basis of 16s rrna gene sequence analysis. dna-dna hybridization experiments with its phylogenetic ...200717625197
effects of mangifera indica l. aqueous extract (vimang) on primary culture of rat hepatocytes.vimang is an aqueous extract from stem bark of mangifera indica l. (mango) with pharmacological properties. it is a mixture of polyphenols (as main components), terpenoids, steroids, fatty acids and microelements. in the present work we studied the cytotoxic effects of vimang on rat hepatocytes, possible interactions of the extract with drug-metabolizing enzymes and its effects on gsh levels and lipid peroxidation. no cytotoxic effects were observed after 24 h exposure to vimang of up to 1000 mi ...200717651882
ecological niche of cryptococcus neoformans var. grubii and cryptococcus gattii in decaying wood of trunk hollows of living trees in jabalpur city of central india.cryptococcus neoformans var. grubii and c. gattii were repeatedly isolated from decaying wood of trunk hollows in living trees growing in jabalpur city in central india. the isolation of c. gattii has been reported from decayed wood inside trunk hollow of tamarindus indica (15.6%), mangifera indica (2.2%), pithecolobium dulce (12.5%), syzygium cumini (14%), and one from bark of s. cumini. c. n. var. grubii was isolated from decaying wood debris of t. indica (4.4%), m. indica (13.3%), terminalia ...200717661160
preventing hepatocyte oxidative stress cytotoxicity with mangifera indica l. extract (vimang).vimang is an aqueous extract of mangifera indica used in cuba to improve the quality of life in patients suffering from inflammatory diseases. in the present study we evaluated the effects of vimang at preventing reactive oxygen species (ros) formation and lipid peroxidation in intact isolated rat hepatocytes. vimang at 20, 50 and 100 microg/ml inhibited hepatocyte ros formation induced by glucose-glucose oxidase. hepatocyte cytotoxicity and lipid peroxidation induced by cumene hydroperoxide was ...200516086553
influence of food acidulants on bioaccessibility of zinc and iron from selected food grains.four common acidulants in indian dietary, i. e., citric acid, tamarind (tamarindus indica), amchur (mangifera indica), and kokum (garcinia indica) were examined for a possible influence on the bioaccessibility of zinc from selected food grains. among the four acidulants examined, amchur and citric acid generally enhanced the bioaccessibility of zinc and iron from all the food grains studied. the increase in zinc bioaccessibility produced by citric acid was around 40% in rice and chickpea, while ...200516189798
antiinflammatory, analgesic and hypoglycemic effects of mangifera indica linn. (anacardiaceae) stem-bark aqueous extract.previous studies in our laboratories and elsewhere have shown that some members of anacardiaceae family possess antiinflammatory, analgesic and hypoglycemic effects in man and mammalian experimental animals. the present study was, therefore, undertaken to examine the antiinflammatory, analgesic and antidiabetic properties of the stem-bark aqueous extract of mangifera indica linn., m. indica a member of the anacardiaceae family, in rats and mice. the stem-bark powder of m. indica was soxhlet extr ...200516273134
an evaluation of some trinidadian plant extracts against larvae of aedes aegypti mosquitoes.in recent times, bioprospecting for plants that show bioactive properties has yielded many chemicals that can be used in controlling mosquitoes. crude extracts of 4 terrestrial and 3 mangrove plants were assayed against 2-3 larval instars of aedes aegypti. among the plants tested, cordia curassavica showed the highest levels of activity for all the extracts tested. azadirachta indica showed the least activity, whereas the 2 cultivars of mangifera indica showed substantial activity for the aqueou ...200717847850
gastroprotective effect of mangiferin, a xanthonoid from mangifera indica, against gastric injury induced by ethanol and indomethacin in rodents.in search of novel gastroprotective agents, mangiferin, a naturally occurring glucosylxanthone from mangifera indica l. (anacardiaceae), was evaluated in mice on gastric injury induced by ethanol and indomethacin. the effects of mangiferin on gastric mucosal damage were assessed by determination of changes in mean gastric lesion area or ulcer score in mice and on gastric secretory volume and total acidity in 4-h pylorus-ligated rats. mangiferin (3, 10 and 30 mg/kg, p. o.) significantly attenuate ...200717918041
cloning and characterization of differentially expressed genes of internal breakdown in mango fruit (mangifera indica).internal breakdown in mango fruits has become a major concern in recent years. this disorder renders the fruits unfit for human consumption. the overall loss due to this disorder is about 35-55%. environmental and physiological factors like high temperature, humidity, respiration and low transpiration rates have been attributed to cause spongy tissue due to reduced loss of heat from fruits. biochemical studies have shown that there is a reduction in ph, total soluble solids, ascorbic acid, total ...200616307821
mangifera indica l. (vimang) protection against serum oxidative stress in elderly humans.we searched for the protective effect of a natural extract from stem bark of mangifera indica l. extract (vimang) on age-related oxidative stress.200616314203
anti-allergic properties of mangifera indica l. extract (vimang) and contribution of its glucosylxanthone mangiferin.vimang is the brand name of formulations containing an extract of mangifera indica l., ethnopharmacologically used in cuba for the treatment of some immunopathological disorders, including bronchial asthma, atopic dermatitis and other allergic diseases. however, the effects of vimang on allergic response have not been reported until now. in this study, the effects of vimang and mangiferin, a c-glucosylxanthone isolated from the extract, on different parameters of allergic response are reported. ...200616536907
effect of mango weevil (coleoptera: curculionidae) damage on mango seed viability in tanzania.studies were conducted at the horticulture unit of sokoine university of agriculture, morogoro, tanzania, to assess the incidence and effect of mango weevil, cryptorhynchus mangiferae (f.) (coleoptera: curculionidae), infestation on mango, mangifera indica l., seed viability. three polyembryo mango cultivars ('sindano nyeusi', 'sindano nyeupe', and 'dodo') as well as three monoembryo mango cultivars ('ex-horticulture', 'tango', and 'bongwa') were collected and examined for the presence of c. man ...200818330124
role of mangiferin on biochemical alterations and antioxidant status in isoproterenol-induced myocardial infarction in rats.the current study dealt with the protective role of mangiferin, a polyphenol from mangifera indica linn. (anacardiaceae), on isoproterenol (isph)-induced myocardial infarction (mi) in rats through its antioxidative mechanism. subcutaneous injection of isph (200 mg/kg body weight in 1 ml saline) to rats for 2 consecutive days caused myocardial damage in rat heart, which was determined by the increased activity of serum lactate dehydrogenase (ldh) and creatine phosphokinase isoenzymes (ck-mb), inc ...200616584858
protective effects of a standard extract of mangifera indica l. (vimang) against mouse ear edemas and its inhibition of eicosanoid production in j774 murine macrophages.a standard aqueous extract of mangifera indica l., used in cuba as antioxidant under the brand name vimang, was tested in vivo for its anti-inflammatory activity, using commonly accepted assays. the standard extract of m. indica, administered orally (50-200mg/kg body wt.), reduced ear edema induced by arachidonic acid (aa) and phorbol myristate acetate (pma) in mice. in the pma model, m. indica extract also reduced myeloperoxidase (mpo) activity. in vitro studies were performed using macrophage ...200616716910
modulation of eosinophil generation and migration by mangifera indica l. extract (vimang).the effects of vimang, an aqueous extract of the stem bark of mangifera indica l. (anacardiaceae), on cell migration in an experimental model of asthma was investigated. in vivo treatment of toxocara canis-infected balb/c mice for 18 days with 50 mg/kg vimang reduced eosinophil migration into the bronchoalveolar space and peritoneal cavity. also, eosinophil generation in bone marrow and blood eosinophilia were inhibited in infected mice treated with vimang. this reduction was associated with inh ...200616846846
evaluation of the genotoxic potential of mangifera indica l. extract (vimang), a new natural product with antioxidant activity.mangifera indica l. extract (vimang) consists of a defined mixture of components (polyphenols, terpenoids, steroids, fatty acids and microelements). it contains a variety of polyphenols, phenolic esters, flavan-3-ols and a xanthone (mangiferin), as main component. this extract has antioxidant action, antitumor and immunemodulatory effects proved in experimental models in both in vitro and in vivo assays. the present study was performed to investigate the genotoxicity potential activity of vimang ...200616857303
interaction of vimang (mangifera indica l. extract) with fe(iii) improves its antioxidant and cytoprotecting activity.a standard aqueous stem bark extract from selected species of mangifera indica l. (anacardiaceae)--vimang, whose major polyphenolic component is mangiferin, displays potent in vitro and in vivo antioxidant activity. the present study provides evidence that the vimang-fe(iii) mixture is more effective at scavenging 2,2-diphenyl-1-picrylhydrazyl (dpph) and superoxide radicals, as well as in protecting against t-butyl hydroperoxide-induced mitochondrial lipid peroxidation and hypoxia/reoxygenation- ...200617000117
analysis of the feeding habits of the swallow-tailed hummingbird, eupetomena macroura (gmelin, 1788), in an urban park in southeastern brazil.the aim of this work was to observe and describe the feeding habits and available food resources of the swallow-tailed hummingbird, eupetomena macroura. the study was carried out in a municipal park located in the city of taubaté, in the state of são paulo. the observations took place between december 2003 and october 2004, recording the following variables: 1) the plant species visited for feeding and territorial defense; 2) the kinds of food resources; and 3) the kinds of flight to procure and ...200818660974
first record of sinoxylon conigerum gerstäcker (coleoptera: bostrichidae) in brazil.this is the first register of sinoxylon conigerum attacking timber of teak (tectona grandis l.f.), mango tree (mangifera indica l.) and (astronium fraxinifolium schott) in the municipalities of cuiabá and várzea grande, state of mato grosso, brazil.200617144148
[potential allelopathic effects of piper nigrum, mangifera indica and clausena lansium].with piper nigrum, mangifera indica and clausena lansium as the donators, this paper studied their potential allelopathic effects on the germination and growth of zea mays, glycine max, cucurbita moschata, arachis hypogaea, raphanus sativus, echinochloa crusgalli, digitaria sanguinalis and stylosanthes guianensis. the results showed that the aqueous extracts of these donators could inhibit the germination and growth of z. mays, g. max, c. moschata, e. crus-galli and d. sanguinalis at high concen ...200617147171
efficacy of azadirachta indica extracts against boophilus microplus.extracts were prepared from leaf, bark, and seed of azadirachta indica, leaf and seed of prunus persica, bark of mangifera indica, and leaf of psidium guajava and were evaluated against boophilus microplus. of the eight extracts screened, the extracts prepared from the a. indica seed showed very high level of efficacy (80%) after 5 h of treatment. besides the immediate effect on adult ticks, the egg-laying properties of the survived ticks was also assessed, and a significant reduction (p < 0.01) ...200818769940
effect of mangifera indica kernel as a feed additive on immunity and resistance to aeromonas hydrophila in labeo rohita fingerlings.the study evaluated the efficacy of dietary doses of mangifera indica (mango) kernel on the immune response and disease resistance of labeo rohita fingerlings against the bacterial pathogen aeromonas hydrophila infections in. l. rohita fingerlings fed diet containing 0 (control), 1g, 5 g, 10 g mango kernel kg(-1) dry diet for 60 days. biochemical (serum total protein, albumin, globulin, albumin:globulin ratio, blood glucose), haematological (wbc, rbc, haemoglobin content) and immunological (supe ...200717161956
protective effect of mangifera indica l. polyphenols on human t lymphocytes against activation-induced cell death.activation-induced cell death (aicd) plays an important role in maintenance of peripheral lymphocyte homeostasis. reactive oxygen species (ros) combined with simultaneous calcium (ca(2+)) influx into the cytosol are required for induction of aicd. the extract obtained from the stem bark of mangifera indica l. has shown to protect t cells from in vitro aicd. this extract is rich in polyphenolic compounds, the three main components of which are mangiferin (ma), catechin (c) and epicatechin (ec). t ...200717184998
comparison of major and trace element concentrations in 16 varieties of cuban mango stem bark (mangifera indica l.).an aqueous decoction of mango (mangifera indica l.) stem bark (msb) has been developed in cuba on an industrial scale to be used as a nutritional supplement, cosmetic, and phytomedicine, with antioxidant, anti-inflammatory, analgesic, and immunomodulatory properties. the concentration of major and trace elements was determined for 16 varieties of msb belonging to two cultivars and grown in cuba in the same soil (red ferralytic). plants were classified into two groups, according to the tree age ( ...200717311401
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