| cloning and heterologous expression of a second (+)-delta-cadinene synthase from gossypium arboreum. | screening of a gossypium arboreum l. cv. nanking cdna library resulted in the identification and cloning of a second (+)-delta-cadinene synthase. a probe for the screens was prepared by pcr using primers based on conserved sequences in farnesyl diphosphate cyclases and genomic dna as a template. this second cdna clone encodes a protein that is 80% identical to the recently described (+)-delta-cadinene synthases cad1-c1 and c14 from g. arboreum and maintains a significant degree of homology to th ... | 1996 | 8904844 |
| anti-salmonella activity of medicinal plants from cameroon. | to evaluate the effects of herbal extracts derived from plants commonly prescribed by traditional practitioners for the treatment of typhoid fever. | 2001 | 12201022 |
| vdnep, an elicitor from verticillium dahliae, induces cotton plant wilting. | verticillium wilt is a vascular disease of cotton. the causal fungus, verticillium dahliae, secretes elicitors in culture. we have generated approximately 1,000 5'-terminal expressed sequence tags (ests) from a cultured mycelium of v. dahliae. a number of ests were found to encode proteins harboring putative signal peptides for secretion, and their cdnas were isolated. heterologous expression led to the identification of a protein with elicitor activities. this protein, named v. dahliae necrosis ... | 2004 | 15294839 |
| efficacy of herbal remedies used by herbalists in oyo state nigeria for treatment of plasmodium falciparum infections--a survey and an observation. | in the course of evaluating the contribution of phytomedicine to possible drug discovery of antimalarial drugs, an ethnomedical survey of specialized children traditional clinics was done. in the observational multi center study, efficacy of eight different herbal remedies, each consisting of 3-8 ingredients and administered by herbalists were investigated in clients enrolled in the six traditional clinics in oyo (urban center) and otu (rural center) of oyo state, nigeria. the clients, aged betw ... | 2004 | 15565927 |
| characterization of gawrky1, a cotton transcription factor that regulates the sesquiterpene synthase gene (+)-delta-cadinene synthase-a. | the cotton (+)-delta-cadinene synthase (cad1), a sesquiterpene cyclase, catalyzes a branch-point step leading to biosynthesis of sesquiterpene phytoalexins, including gossypol. cad1-a is a member of cad1 gene family, and its promoter contains a w-box palindrome with two reversely oriented tgac repeats, which are the proposed binding sites of wrky transcription factors. we isolated several wrky cdnas from gossypium arboreum. one of them, gawrky1, encodes a protein containing a single wrky domain ... | 2004 | 15133151 |
| molecular cloning, differential expression, and functional characterization of a family of class i ubiquitin-conjugating enzyme (e2) genes in cotton (gossypium). | two cdnas and their corresponding genes (ghubc1 and ghubc2) encoding ubiquitin-conjugating enzymes (e2s) have been cloned and characterized from allotetraploid cotton gossypium hirsutum ((ad)(1) genome). three additional e2 genes (gaubc1, gtubc2, and grubc2) have also been identified from diploid cottons gossypium arboreum (a(2) genome), gossypium thurberi (d(1) genome), and gossypium raimondii (d(5) genome), respectively. the derived amino acid sequences of the five closely related cotton e2s a ... | 2003 | 12591614 |
| molecular cloning and functional identification of (+)-delta-cadinene-8-hydroxylase, a cytochrome p450 mono-oxygenase (cyp706b1) of cotton sesquiterpene biosynthesis. | in cotton, gossypol and related sesquiterpene aldehydes are present in the glands of aerial tissues and in epidermal cells of roots. a cytochrome p450 was found to be expressed in aerial tissues of glanded cotton cultivars, but not or at an extremely low level in the aerial tissues of a glandless cultivar. its cdna was then isolated from gossypium arboreum l. after expression in saccharomyces cerevisiae, the p450 was found to catalyse the hydroxylation of (+)-delta-cadinene, forming 8-hydroxy-(+ ... | 2001 | 11696190 |
| glucosylceramide synthases, a gene family responsible for the biosynthesis of glucosphingolipids in animals, plants, and fungi. | glucosylceramides are membrane lipids in most eukaryotic organisms and in a few bacteria. the physiological functions of these glycolipids have only been documented in mammalian cells, whereas very little information is available of their roles in plants, fungi, and bacteria. in an attempt to establish appropriate experimental systems to study glucosylceramide functions in these organisms, we performed a systematic functional analysis of a glycosyltransferase gene family with members of animal, ... | 2001 | 11443131 |
| the cyclization of farnesyl diphosphate and nerolidyl diphosphate by a purified recombinant delta-cadinene synthase. | the first step in the conversion of the isoprenoid intermediate, farnesyl diphosphate (fdp), to sesquiterpene phytoalexins in cotton (gossypium barbadense) plants is catalyzed by delta-cadinene (cdn) synthase. cdn is the precursor of desoxyhemigossypol and hemigossypol defense sesquiterpenes. in this paper we have studied the mechanism for the cyclization of fdp and the putative intermediate, nerolidyl diphosphate, to cdn. a purified recombinant cdn synthase (cdn1-c1) expressed in escherichia co ... | 2001 | 11299356 |
| expression pattern of (+)-delta-cadinene synthase genes and biosynthesis of sesquiterpene aldehydes in plants of gossypium arboreum l. | the cotton (+)-delta-cadinene synthase, a sesquiterpene cyclase, is encoded by a complex gene family which, based on homology, can be divided into two subfamilies: cad1-a and cad1-c. southern blots revealed several members of the cad1-c subfamily, and a single member of the cad1-a subfamily, in the diploid gossypium arboreum genome. one of the cad1-c genes, cad1-c3, was isolated from this species. according to reverse transcriptase-polymerase chain reaction, transcripts of both cad1-c and cad1-a ... | 2000 | 10787059 |
| cloning, expression, and characterization of (+)-delta-cadinene synthase: a catalyst for cotton phytoalexin biosynthesis. | in cotton, sesquiterpene phytoalexins are elicited in response to bacterial or fungal infection. a gossypium arboreum cell suspension culture which produces the sesquiterpene phytoalexin gossypol showed a time-dependent 10-fold increase in a 1.9-kb mrna in response to a challenge by a preparation from verticillium dahliae. the mrna prepared from these elicited cultures was used to isolated two cdna clones that contain open frames coding for proteins of 554 amino acids with m(r) 64,096 and 64,118 ... | 1995 | 8554317 |
| cottonseed extract versus pharmamedia for the in vitro mould-yeast conversion of blastomyces dermatitidis. | a cottonseed medium, based on a 1% aqueous extract of seeds of any of the eight indigenously available varieties of cotton belonging to gossypium hirsutum or gossypium arboreum, was evaluated for the mould-yeast conversion of blastomyces dermatitidis in vitro, and compared with pharmamedia agar as a control. the cottonseed agar was found to be as efficient as pharmamedia agar for the mould-yeast conversion of the 19 b. dermatitidis strains tested. therefore, cottonseeds provide an adequate and i ... | 1990 | 2380881 |
| salicylic acid-related cotton (gossypium arboreum) ribosomal protein garpl18 contributes to resistance to verticillium dahliae. | verticillium dahliae is a phytopathogenic fungal pathogen that causes vascular wilt diseases responsible for considerable decreases in cotton yields. the complex mechanism underlying cotton resistance to verticillium wilt remains uncharacterized. identifying an endogenous resistance gene may be useful for controlling this disease. | 2017 | 28253842 |
| identification and characterization of cotton genes involved in fuzz-fiber development. | cotton fuzz fibers, like arabidopsis trichomes, are elongated unicells. it is postulated that a transcriptional complex of glabra1 (gl1), glabra3 (gl3), and transparent testaglabra1 (ttg1) might be in existence in arabidopsis as evidenced by their physical interaction in yeast, and the complex regulates expression of glabra2 (gl2) controlling trichome cell differentiation; it is also assumed that triptychon (try) and caprice (cpc) counteract the complex formation in neighboring cells. here, the ... | 2013 | 23710824 |
| transcriptome analysis reveals long noncoding rnas involved in fiber development in cotton (gossypium arboreum). | long noncoding rnas (lncrnas) play important roles in various biological regulatory processes in yeast, mammals, and plants. however, no systematic identification of lncrnas has been reported in gossypium arboreum. in this study, the strand-specific rna sequencing (ssrna-seq) of samples from cotton fibers and leaves was performed, and lncrnas involved in fiber initiation and elongation processes were systematically identified and analyzed. we identified 5,996 lncrnas, of which 3,510 and 2,486 ca ... | 2016 | 26803302 |
| the hd-zip iv gene gahox1 from cotton is a functional homologue of the arabidopsis glabra2. | most of the plant homeodomain-containing proteins play important roles in organ patterning and development, and arabidopsis glabra2 (gl2), a member of the class iv homeodomain-leucine zipper (hd-zip) proteins, is a trichome and non-root hair cell regulator. here we report the analysis of two cotton homeodomain-containing proteins, gahox1 and gahox2, isolated from the diploid cotton gossypium arboreum. both gahox1 and gahox2 belong to the class iv hd-zip family. when expressed under the control o ... | 2008 | 18507789 |
| gossypium arboreum ghsp26 enhances drought tolerance in gossypium hirsutum. | heat-shock proteins (hsp) are molecular chaperones for protein molecules. these proteins play an important role in protein-protein interactions such as, folding and assisting in the establishment of proper protein conformation and prevention of unwanted protein aggregation. a small hsp gene ghsp26 present in gossypium arboreum responds to dehydration. in the present study, an attempt was made to overcome the problem of drought stress in cotton. a cdna of ghsp26 was isolated from g. arboreum, clo ... | 2011 | 19847887 |
| asiatic cotton can generate similar economic benefits to bt cotton under rainfed conditions. | american cotton (gossypium hirsutum l.), transformed with bacillus thuringiensis cry genes (bt g. hirsutum) that confer resistance to lepidopteran pests, is extensively cultivated worldwide. in india, transgenic bt g. hirsutum was commercially released in 2002 and by 2014 95% of farmers had adopted bt g. hirsutum(1). the economic benefits of bt g. hirsutum over non-bt g. hirsutum are well documented and include increase in yields, increase in farmers' net revenue and reduction in pesticide appli ... | 2015 | 27250007 |
| introgression of cotton leaf curl virus-resistant genes from asiatic cotton (gossypium arboreum) into upland cotton (g. hirsutum). | cotton is under the constant threat of leaf curl virus, which is a major constraint for successful production of cotton in the pakistan. a total of 3338 cotton genotypes belonging to different research stations were screened, but none were found to be resistant against the burewala strain of cotton leaf curl virus (clcuv). we explored the possibility of transferring virus-resistant genes from gossypium arboreum (2n = 26) into g. hirsutum (2n = 52) through conventional breeding techniques. ... | 2011 | 22002133 |
| inhibitor and substrate activities of sesquiterpene olefins toward +-δ-cadinene-8-hydroxylase, a cytochrome p450 monooxygenase (cyp706b1). | several lines of evidence indicate that (+)-δ-cadinene-8-hydroxylase (cyp706b1) plays an important role in biosynthesis of gossypol in gossypium arboreum l. (luo et al., 2001; wang et al., 2003). the catalytically active enzyme has been expressed in yeast microsomes. some microsomal preparations conjugated the hydroxylated (+)-δ-cadinene to a moiety that has not yet been identified. however, when microsomes were treated with n-octyl-β-d-glucoside (og), a non-ionic detergent, (+)-δ-cadinene was r ... | 2010 | 20801466 |
| identification and genomic location of a reniform nematode (rotylenchulus reniformis) resistance locus (ren ari) introgressed from gossypium aridum into upland cotton (g. hirsutum). | in this association mapping study, a tri-species hybrid, [gossypium arboreum x (g. hirsutum x g. aridum)(2)], was crossed with md51ne (g. hirsutum) and progeny from the cross were used to identify and map ssr markers associated with reniform nematode (rotylenchulus reniformis) resistance. seventy-six progeny (the 50 most resistant and 26 most susceptible) plants were genotyped with 104 markers. twenty-five markers were associated with a resistance locus that we designated ren(ari) and two marker ... | 2009 | 19830404 |
| resistance and resistant reaction of gossypium arboreum to the reniform, nematode, rotylenchulus reniformis. | gossypium arboreum 'nanking cb 1402' possessed a high level of resistance to rotylenchulus reniformis. within 16 h, the nematode penetrated roots of resistant and susceptible cottons equally. after 36 h, significantly fewer nematodes were found in resistant roots. larvae fed in either an endodermal or pericyclic cell and had no specificity for root tissue of a particular age. in roots of resistant g. arboreum '1402,' wall breakdown of pericyclic cells was evident after 3 d, endodermal and cortic ... | 1981 | 19300777 |
| suppression of rotylenchulus reniformis 122-cm deep endorses resistance introgression in gossypium. | nine sources of resistance to rotylenchulus reniformis in gossypium (cotton) were tested by measuring population density (pf) and root-length density 0 to 122 cm deep. a pf in the plow layer less than the autumn sample treatment threshold used by consultants was considered the minimum criterion for acceptable resistance, regardless of population density at planting (pi). other criteria were ample roots and a pf lower than on the susceptible control, as in pot studies. in a texas field in 2001 an ... | 2006 | 19259448 |
| functional analysis of cotton small heat shock protein promoter region in response to abiotic stresses in tobacco using agrobacterium-mediated transient assay. | the cotton (gossypium arboreum) stress-related gene ghsp26 responds to dehydration. to elucidate its stress tolerant mechanism at the transcriptional level, we isolated and characterized the promoter region (pghsp26, -2,831 bp) flanking the 5' ghsp26 coding region from the genomic dna. a series of pghsp26 deletion derivatives was created for the identification of the upstream region of the gene required for the promoter activity. each deletion construct was analyzed by agrobacterium mediated tra ... | 2009 | 18991019 |
| antibacterial, antioxidant and fibroblast growth stimulation of aqueous extracts of ficus asperifolia miq. and gossypium arboreum l., wound-healing plants of ghana. | use of in vitro tests to search for relevant activities in bark of ficus asperifolia miq. and leaves of gossypium arboreum l., used in ghana for wound healing. | 2008 | 18638536 |
| transposable elements play an important role during cotton genome evolution and fiber cell development. | transposable elements (tes) usually occupy largest fractions of plant genome and are also the most variable part of the structure. although traditionally it is hallmarked as "junk and selfish dna", today more and more evidence points out te's participation in gene regulations including gene mutation, duplication, movement and novel gene creation via genetic and epigenetic mechanisms. the recently sequenced genomes of diploid cottons gossypium arboreum (aa) and gossypium raimondii (dd) together w ... | 2016 | 26687725 |
| independent domestication of two old world cotton species. | domesticated cotton species provide raw material for the majority of the world's textile industry. two independent domestication events have been identified in allopolyploid cotton, one in upland cotton (gossypium hirsutum l.) and the other to egyptian cotton (gossypium barbadense l.). however, two diploid cotton species, gossypium arboreum l. and gossypium herbaceum l., have been cultivated for several millennia, but their status as independent domesticates has long been in question. using geno ... | 2016 | 27289095 |
| characterization of the global transcriptome for cotton (gossypium hirsutum l.) anther and development of ssr marker. | cotton is an important fiber plant, and it's attractive to elucidate the molecular mechanism of anther development due to the close relationship between the anther fertility and boll-setting, and also fiber yield. in the present paper, 47.2 million paired-end reads with average length of 82.87 bp from the anthers of tm-1 (gossypium hirsutum l.), a genetic standard line, were generated through transcriptome sequencing, and 210,965 unigenes of more than 100 bp were obtained. blast, kegg, cog, and ... | 2014 | 25178523 |
| expression pattern of genes encoding farnesyl diphosphate synthase and sesquiterpene cyclase in cotton suspension-cultured cells treated with fungal elicitors. | cotton plants accumulate sesquiterpene aldehydes in pigment glands. the two enzymes farnesyl diphosphate synthase (fps) and (+)-delta-cadinene synthase (cad), a sesquiterpene cyclase, are involved in the biosynthesis of these secondary metabolites. a full-length cdna (garfps) encoding fps was isolated from gossypium arboreum and identified by in vitro enzymatic assay of the garfps protein heterologously expressed in escherichia coli. treatment of g. arboreum suspension-cultured cells with an eli ... | 1999 | 10624017 |
| gossypium arboreum accessions resistant to rotylenchulus reniformis. | in the southeastern united states, reniform nematode (rotylenchulus reniformis) is a serious pest of upland cotton (gossypium hirsutum), a species which has no naturally occurring resistance against this nematode. to identify sources of reniform nematode resistance in species closely related to upland cotton, 222 g. arboreum accessions from the u.s. germplasm collection were evaluated in repeated growth chamber experiments. in initial screenings, root infection was measured 4 wks after inoculati ... | 2016 | 28154428 |
| insights into the evolution of cotton diploids and polyploids from whole-genome re-sequencing. | understanding the composition, evolution, and function of the gossypium hirsutum (cotton) genome is complicated by the joint presence of two genomes in its nucleus (at and dt genomes). these two genomes were derived from progenitor a-genome and d-genome diploids involved in ancestral allopolyploidization. to better understand the allopolyploid genome, we re-sequenced the genomes of extant diploid relatives that contain the a1 (gossypium herbaceum), a2 (gossypium arboreum), or d5 (gossypium raimo ... | 2013 | 23979935 |
| the comparative genetics of gossypium hirsutum l. and the synthetic amphiloid, gossypium arboreum l. x gossypium thurberi tod. | | 1962 | 13898581 |
| chromosome structural changes in diploid and tetraploid a genomes of gossypium. | the genus gossypium, which comprises a divergent group of diploid species and several recently formed allotetraploids, offers an excellent opportunity to study polyploid genome evolution. in this study, chromosome structural variation among the a, at, and d genomes of gossypium was evaluated by comparative genetic linkage mapping. we constructed a fully resolved rflp linkage map for the diploid a genome consisting of 275 loci using an f2 interspecific gossypium arboreum x gossypium herbaceum fam ... | 2006 | 16699553 |
| new world tetraploid cottons contain old world cytoplasm. | new world tetraploid cottons (gossypium spp.) originated through hybridization of ancestral diploid species that presently have allopatric ranges in asia-africa (the a genome) and the new world tropics and subtropics (the d genome). despite intensive study, the identity of the parental diploids and the antiquity of polyploidization remain unresolved. in this study, variation in the maternally inherited chloroplast genome was assessed among species representing both of the parental genomes and th ... | 1989 | 16594050 |
| cloning and functional analysis of the promoter of an ascorbate oxidase gene from gossypium hirsutum. | apoplastic ascorbate oxidase (ao) plays significant roles in plant cell growth. however, the mechanism of underlying the transcriptional regulation of ao in gossypium hirsutum remains unclear. here, we obtained a 1,920-bp promoter sequence from the gossypium hirsutum ascorbate oxidase (ghao1) gene, and this ghao1 promoter included a number of known cis-elements. promoter activity analysis in overexpressing pghao1::gfp-gus tobacco (nicotiana benthamiana) showed that the ghao1 promoter exhibited h ... | 2016 | 27597995 |
| genome-wide comparative analysis of the phospholipase d gene families among allotetraploid cotton and its diploid progenitors. | in this study, 40 phospholipase d (pld) genes were identified from allotetraploid cotton gossypium hirsutum, and 20 pld genes were examined in diploid cotton gossypium raimondii. combining with 19 previously identified gossypium arboreum pld genes, a comparative analysis was performed among the pld gene families among allotetraploid and two diploid cottons. based on the orthologous relationships, we found that almost each g. hirsutum pld had a corresponding homolog in the g. arboreum and g. raim ... | 2016 | 27213891 |
| diversity in betasatellites associated with cotton leaf curl disease during source-to-sink movement through a resistant host. | cotton leaf curl is devastating disease of cotton characterized by leaf curling, vein darkening and enations. the disease symptoms are induced by dna satellite known as cotton leaf curl multan betasatellite (clcumub), dominant betasatellite in cotton but another betasatellite known as chili leaf curl betasatellite (chlcb) is also found associated with the disease. grafting experiment was performed to determine if host plant resistance is determinant of dominant population of betasatellite in cot ... | 2016 | 26889114 |
| delineating the glycoproteome of elongating cotton fiber cells. | the data presented here delineates the glycoproteome component in the elongating cotton fiber cells attained using complementary proteomic approaches followed by protein and n-linked glycosylation site identification (kumar et al., 2013) [1]. utilizing species specific protein sequence databases in proteomic approaches often leads to additional information that may not be obtained using cross-species databases. in this context we have reanalyzed our glycoproteome dataset with the gossypium arbor ... | 2015 | 26693171 |
| the complexity of posttranscriptional small rna regulatory networks revealed by in silico analysis of gossypium arboreum l. leaf, flower and boll small regulatory rnas. | micrornas (mirnas) and secondary small interfering rnas (principally phased sirnas or trans-acting sirnas) are two distinct subfamilies of small rnas (srnas) that are emerging as key regulators of posttranscriptional gene expression in plants. both mirnas and secondary-sirnas (sec-sirnas) are processed from longer rna precursors by dicer-like proteins (dcls). gossypium arboreum l., also known as tree cotton or asian cotton, is a diploid, possibly ancestral relative of tetraploid gossypium hirsut ... | 2015 | 26070200 |
| a new synthetic amphiploid (aaddaa) between gossypium hirsutum and g. arboreum lays the foundation for transferring resistances to verticillium and drought. | gossypium arboreum, a cultivated cotton species (2n = 26, aa) native to asia, possesses invaluable characteristics unavailable in the tetraploid cultivated cotton gene pool, such as resistance to pests and diseases and tolerance to abiotic stresses. however, it is quite difficult to transfer favorable traits into upland cotton through conventional methods due to the cross-incompatibility of g. hirsutum (2n = 52, aadd) and g. arboreum. here, we improved an embryo rescue technique to overcome the ... | 2015 | 26061996 |
| genome sequence of cultivated upland cotton (gossypium hirsutum tm-1) provides insights into genome evolution. | gossypium hirsutum has proven difficult to sequence owing to its complex allotetraploid (atdt) genome. here we produce a draft genome using 181-fold paired-end sequences assisted by fivefold bac-to-bac sequences and a high-resolution genetic map. in our assembly 88.5% of the 2,173-mb scaffolds, which cover 89.6%∼96.7% of the atdt genome, are anchored and oriented to 26 pseudochromosomes. comparison of this g. hirsutum atdt genome with the already sequenced diploid gossypium arboreum (aa) and gos ... | 2015 | 25893780 |
| genome-wide analysis of the family 1 glycosyltransferases in cotton. | family 1 gt, designated as ugt, is the largest and most functionally important multigene family in the plant kingdom. in this study, we carried out a genome-wide identification, analysis, and comparison of 142, 146, and 196 putative ugts from gossypium raimondii, gossypium arboreum, and gossypium hirsutum, respectively. all members present the 44 amino-acid conserved consensus sequence termed the plant secondary product glycosyltransferase motif. according to the phylogenetic relationship among ... | 2015 | 25851236 |
| upland cotton gene ghfpf1 confers promotion of flowering time and shade-avoidance responses in arabidopsis thaliana. | extensive studies on floral transition in model species have revealed a network of regulatory interactions between proteins that transduce and integrate developmental and environmental signals to promote or inhibit the transition to flowering. previous studies indicated flowering promoting factor 1 (fpf1) gene was involved in the promotion of flowering, but the molecular mechanism was still unclear. here, fpf1 homologous sequences were screened from diploid gossypium raimondii l. (d-genome, n = ... | 2014 | 24626476 |
| distribution and evolution of cotton fiber development genes in the fibreless gossypium raimondii genome. | cotton fiber represents the largest single cell in plants and they serve as models to study cell development. this study investigated the distribution and evolution of fiber unigenes anchored to recombination hotspots between tetraploid cotton (gossypium hirsutum) at and dt subgenomes, and within a parental diploid cotton (gossypium raimondii) d genome. comparative analysis of at vs d and dt vs d showed that 1) the d genome provides many fiber genes after its merger with another parental diploid ... | 2015 | 25796538 |
| rnai construct of a cytochrome p450 gene cyp82d109 blocks an early step in the biosynthesis of hemigossypolone and gossypol in transgenic cotton plants. | naturally occurring terpenoid aldehydes from cotton, such as hemigossypol, gossypol, hemigossypolone, and the heliocides, are important components of disease and herbivory resistance in cotton. these terpenoids are predominantly found in the glands. differential screening identified a cytochrome p450 cdna clone (cyp82d109) from a gossypium hirsutum cultivar that hybridized to mrna from glanded cotton but not glandless cotton. both the d genome cotton gossypium raimondii and a genome cotton gossy ... | 2015 | 25794893 |
| ex planta phytoremediation of trichlorophenol and phenolic allelochemicals via an engineered secretory laccase. | plant roots release a range of enzymes capable of degrading chemical compounds in their immediate vicinity. we present a system of phytoremediation ex planta based on the overexpression of one such enzyme, a secretory laccase. laccases catalyze the oxidation of a broad range of phenolic compounds, including polychlorinated phenols such as 2,4,6-trichlorophenol (tcp), that are among the most hazardous and recalcitrant pollutants in the environment. we isolated a secretory laccase cdna of lac1, wh ... | 2004 | 15195102 |
| evaluation of cotton leaf curl virus resistance in bc1, bc2, and bc3 progenies from an interspecific cross between gossypium arboreum and gossypium hirsutum. | cotton leaf curl virus disease (clcud) is an important constraint to cotton production. the resistance of g. arboreum to this devastating disease is well documented. in the present investigation, we explored the possibility of transferring genes for resistance to clcud from g. arboreum (2n = 26) cv 15-mollisoni into g. hirsutum (2n = 52) cv crsm-38 through conventional breeding. we investigated the cytology of the bc1 to bc3 progenies of direct and reciprocal crosses of g. arboreum and g. hirsut ... | 2014 | 25372141 |
| identification of micro-rnas in cotton. | the plant genome has conserved small non-coding micrornas (mirnas) genes about 20-24 nucleotides long. they play a vital role in the gene regulation at various stages of plant life. their conserved nature among the various organisms not only suggests their early evolution in eukaryotes but also makes them a good source of new mirna discovery by homology search using bioinformatics tools. a systematic search approach was used for interspecies orthologues of mirna precursors, from known sequences ... | 2016 | 18603441 |
| genetic mapping of new cotton fiber loci using est-derived microsatellites in an interspecific recombinant inbred line cotton population. | there is an immediate need for a high-density genetic map of cotton anchored with fiber genes to facilitate marker-assisted selection (mas) for improved fiber traits. with this goal in mind, genetic mapping with a new set of microsatellite markers [comprising both simple (ssr) and complex (csr) sequence repeat markers] was performed on 183 recombinant inbred lines (rils) developed from the progeny of the interspecific cross gossypium hirsutum l. cv. tm1 x gossypium barbadense l. pima 3-79. micro ... | 2005 | 16187061 |
| [studies of fish and karyotype of gossypium barbadense]. | based on the fluorescent in situ hybridization (fish) of somatic chromosome of gossypium barbadense with the probe of genomic dna (gdna) of gossypium arboreum, two sets of chromosomes were easily distinguished by signals hybridized or not. the fish directly proved that g. barbadense originated from two different diploid species, but was not in concordance with the former point that every chromosome of a sub-genome of tetraploid species was bigger than that of other sub-genome (d genome). the kar ... | 2001 | 11209715 |
| identification of genes related to salt stress tolerance using intron-length polymorphic markers, association mapping and virus-induced gene silencing in cotton. | intron length polymorphisms (ilps), a type of gene-based functional marker, could themselves be related to the particular traits. here, we developed a genome-wide cotton ilps based on orthologs annotation from two sequenced diploid species, a-genome gossypium arboreum and d-genome g. raimondii. we identified 10,180 putative ilp markers from 5,021 orthologous genes. among these, 535 ilp markers from 9 gene families related to stress were selected for experimental verification. polymorphic rates w ... | 2017 | 28373664 |
| phylogenetic analysis of gossypium l. using restriction fragment length polymorphism of repeated sequences. | cotton is the world's leading textile fiber crop and is also grown as a bioenergy and food crop. knowledge of the phylogeny of closely related species and the genome origin and evolution of polyploid species is significant for advanced genomics research and breeding. we have reconstructed the phylogeny of the cotton genus, gossypium l., and deciphered the genome origin and evolution of its five polyploid species by restriction fragment analysis of repeated sequences. nuclear dna of 84 accessions ... | 2015 | 25877517 |
| genome-wide identification of cbl family and expression analysis of cbls in response to potassium deficiency in cotton. | calcineurin b-like (cbl) proteins, as calcium sensors, play pivotal roles in plant responses to diverse abiotic stresses and in growth and development through interaction with cbl-interacting protein kinases (cipks). however, knowledge about functions and evolution of cbls in gossypium plants is scarce. here, we conducted a genome-wide survey and identified 13, 13 and 22 cbl genes in the progenitor diploid gossypium arboreum and gossypium raimondii, and the cultivated allotetraploid gossypium hi ... | 2017 | 28828254 |
| successive use of non-host plant proteinase inhibitors required for effective inhibition of helicoverpa armigera gut proteinases and larval growth | we report on the efficacy of proteinase inhibitors (pis) from three host plants (chickpea [cicer arietinum], pigeonpea [cajanus cajan], and cotton [gossypium arboreum]) and three non-host (groundnut [arachis hypogea], winged bean [psophocarpus tetragonolobus], and potato [solanum tuberosum]) in retarding the growth of helicoverpa armigera larvae, a devastating pest of important crop plants. enzyme assays and electrophoretic analysis of interaction of h. armigera gut proteinases (hgps) with pis r ... | 1999 | 10517841 |
| identification and profiling of micrornas expressed in elongating cotton fibers using small rna deep sequencing. | plant micrornas (mirnas) have been shown to play essential roles in the regulation of gene expression. in this study, small rna deep sequencing was applied to explore novel mirnas expressed in elongating cotton fibers. a total of 46 novel and 96 known mirnas, primarily derived from the corresponding specific loci in genome of gossypium arboreum, were identified. 64 mirnas were shown to be differentially expressed during the fiber elongation process; 16 were predicted to be novel mirnas while the ... | 2016 | 27909445 |
| coordinated accumulation of (+)-delta-cadinene synthase mrnas and gossypol in developing seeds of gossypium hirsutum and a new member of the cad1 family from g. arboreum. | a new member of the (+)-delta-cadinene synthase (cad1) family was isolated from a gossypium arboreum cdna library. this cdna encodes a protein that showed 97.3%, 96.9%, and 79.2% sequence identities with the proteins encoded by previously isolated cdnas of cad1-c1, cad1-c14, and cad1-a, respectively. it may be grouped into the cad1-c subfamily as cad1-c2. seeds of a glanded cotton cultivar, g. hirsutum cv. sumian-6, were collected at different intervals during maturation, and the cad1 mrna level ... | 1999 | 10075752 |
| effect of age of cell suspension cultures on susceptibility to a fungal elicitor. | fungal elicitor induced phytoalexin formation and the corresponding fluorescence transitions of the molecular probes pyranine and oxonol vi, in soybean (glycine max merr var kent) and cotton (gossypium arboreum l. nanking) cell suspensions were both significantly affected by the age of the cells. during the lag phase and the beginning of the exponential growth phase both cultures exhibited stress responses (i.e. phytoalexin formation and molecular probe fluorescence transitions) in the absence o ... | 1989 | 24240464 |
| transcriptomic profiling of developing fiber in levant cotton (gossypium herbaceum l.). | cotton (gossypium spp.) is an imperative economic crop of the globe due to its natural textile fiber. molecular mechanisms of fiber development have been greatly revealed in allotetraploid cotton but remained unexplored in gossypium herbaceum. g. herbaceum can withstand the rigors of nature like drought and pests but produce coarse lint. this undesirable characteristic strongly needs the knowledge of fiber development at molecular basis. the present study reported the transcriptome sequence of t ... | 2018 | 29332190 |
| a phi-class glutathione s-transferase gene for verticillium wilt resistance in gossypium arboreum identified in a genome-wide association study. | verticillium wilt disease is one of the most destructive biotic stresses faced by cotton plants. here, we performed a genome-wide association study (gwas) in 215 chinese gossypium arboreum accessions inoculated as seedlings with verticillium dahliae to identify candidate loci involved in wilt resistance. we identified 309 loci that had a significant association with verticillium wilt resistance and log (p) values greater than 5.0; the highest signal appeared on ca3 in a 74 kb haplotype block. fi ... | 2017 | 29165718 |
| transcriptomics reveals multiple resistance mechanisms against cotton leaf curl disease in a naturally immune cotton species, gossypium arboreum. | cotton leaf curl disease (clcud), caused by cotton leaf curl viruses (clcuvs), is among the most devastating diseases in cotton. while the widely cultivated cotton species gossypium hirsutum is generally susceptible, the diploid species g. arboreum is a natural source for resistance against clcud. however, the influence of clcud on the g. arboreum transcriptome and the interaction of clcud with g. arboreum remains to be elucidated. here we have used an rna-seq based study to analyze differential ... | 2017 | 29162860 |
| suppression of cotton leaf curl disease symptoms in gossypium hirsutum through over expression of host-encoded mirnas. | cotton leaf curl disease (clcud), a major factor resulting in the enormous yield losses in cotton crop, is caused by a distinct monopartite begomovirus in association with cotton leaf curl multan betasatellite (clcumb). micro(mi)rnas are known to regulate gene expression in eukaryotes, including antiviral defense in plants. in a previous study, we had computationally identified a set of cotton mirnas, which were shown to have potential targets in the genomes of cotton leaf curl multan virus (clc ... | 2017 | 29017848 |
| characterization of conserved circular rna in polyploid gossypium species and their ancestors. | circular rna (circrna) is a regulatory class of long, noncoding rna found in both plant and animal kingdoms. the profile and characterization of circrna in cotton species remains to be explored. here, using 24 rrna-depleted rna-seq libraries of putative diploid progenitors of gossypium spp., gossypium arboreum and gossypium raimondii, their interspecies hybrid (f1 ) and allotetraploid gossypium hirsutum, 1041, 1478, 1311, and 499 circrnas were identified in each cotton species, respectively. a p ... | 2017 | 28971489 |
| cross-cultural analysis of medicinal plants commonly used in ethnoveterinary practices at south waziristan agency and bajaur agency, federally administrated tribal areas (fata), pakistan. | in remote areas, medicinal plants have an imperative role in curing various livestock's ailments. in pakistan, people residing in remote areas including south waziristan agency and bajaur agency depend on traditional herbal remedies for treating their domestic animals. medicinal plants are an important part of the medical system in these agencies. the prime goal of the current study is to explore the ethnoveterinary practices in the two regions and discuss cross-cultural consensus on the use of ... | 2018 | 28917974 |
| transcript profiling of genes expressed during fibre development in diploid cotton (gossypium arboreum l.). | cotton fibre is a single cell and it is one of the best platforms for unraveling the genes express during various stages of fibre development. there are reports devoted to comparative transcriptome study on fiber cell initiation and elongation in tetraploid cultivated cotton. however, in the present investigation, comparative transcriptome study was made in diploid cultivated cotton using isogenic fuzzy-lintless (fl) and normal fuzzy linted (fl) lines belong to gossypium arboreum, diploid specie ... | 2017 | 28859611 |
| genome-wide characterization and expression analysis of the aldehyde dehydrogenase (aldh) gene superfamily under abiotic stresses in cotton. | in plants, aldehyde dehydrogenases (aldhs) function as 'aldehyde scavengers' by removing reactive aldehydes and thus play important roles in stress responses. to date, 30 aldhs have been identified in gossypium raimondii, whereas aldhs have not been studied in gossypium arboreum or in tetraploid cotton. in this study, we identified 30, 59 and 59 aldehyde dehydrogenase (aldh) genes from g. arboreum, g. hirsutum and g. barbadense, respectively. gene structure analysis revealed that members of the ... | 2017 | 28711668 |
| evolution, gene expression profiling and 3d modeling of csld proteins in cotton. | among cesa-like gene superfamily, the cellulose synthase-like d (csld) genes are most similar to cellulose synthase genes and have been reported to be involved in tip-growing cell and stem development. however, there has been no genome-wide characterization of this gene subfamily in cotton. we thus sought to analyze the evolution and functional characterization of csld proteins in cotton based on fully sequenced cotton genomes. | 2017 | 28693426 |
| genome-wide analysis of wox genes in upland cotton and their expression pattern under different stresses. | wuschel-related homeobox (wox) family members play significant roles in plant growth and development, such as in embryo patterning, stem-cell maintenance, and lateral organ formation. the recently published cotton genome sequences allow us to perform comprehensive genome-wide analysis and characterization of wox genes in cotton. | 2017 | 28683794 |
| identification and characterization of mirna transcriptome in asiatic cotton (gossypium arboreum) using high throughput sequencing. | micrornas (mirnas) are small 20-24nt molecules that have been well studied over the past decade due to their important regulatory roles in different cellular processes. the mature sequences are more conserved across vast phylogenetic scales than their precursors and some are conserved within entire kingdoms, hence, their loci and function can be predicted by homology searches. different studies have been performed to elucidate mirnas using de novo prediction methods but due to complex regulatory ... | 2017 | 28663752 |
| genome-wide analysis of the sus gene family in cotton. | sucrose synthase (sus) is a key enzyme in plant sucrose metabolism. in cotton, sus (ec 2.4.1.13) is the main enzyme that degrades sucrose imported into cotton fibers from the phloem of the seed coat. this study demonstrated that the genomes of gossypium arboreum l., g. raimondii ulbr., and g. hirsutum l., contained 8, 8, and 15 sus genes, respectively. their structural organizations, phylogenetic relationships, and expression profiles were characterized. comparisons of genomic and coding sequenc ... | 2013 | 23691964 |
| differential transcript profiling alters regulatory gene expression during the development of gossypium arboreum, g.stocksii and somatic hybrids. | polyploidy or genome doubling (i.e., the presence of two or more diploid parental genome sets within an organism) are very important in higher plants. of particular interest are the mechanisms in the new microenvironment of the common nucleus, where doubled regulatory networks interact to generate a viable genetic system capable of regulating growth, development and responses to the environment. to determine the effects of whole genome merging and doubling on the global gene expression architect ... | 2017 | 28600526 |
| a genome-scale analysis of the pin gene family reveals its functions in cotton fiber development. | the pin-formed (pin) protein, the most important polar auxin transporter, plays a critical role in the distribution of auxin and controls multiple biological processes. however, characterizations and functions of this gene family have not been identified in cotton. here, we identified the pin family in gossypium hirsutum, gossypium arboreum, and gossypium raimondii. this gene family was divided into seven subgroups. a chromosomal distribution analysis showed that ghpin genes were evenly distribu ... | 2017 | 28424725 |
| analyses of the sucrose synthase gene family in cotton: structure, phylogeny and expression patterns. | in plants, sucrose synthase (sus) is widely considered as a key enzyme involved in sucrose metabolism. several paralogous genes encoding different isozymes of sus have been identified and characterized in multiple plant genomes, while limited information of sus genes is available to date for cotton. | 2012 | 22694895 |
| lack of adaptation to a new host in a generalist herbivore: implications for host plant resistance to twospotted spider mites in cotton. | the twospotted spider mite (tetranychus urticae koch) is an important pest of cotton. this pest has a broad host range, but when changing between hosts an initial decline in fitness often occurs. this is usually followed by an increase in fitness after rapid adaptation to the new host, usually within five generations. | 2015 | 24777962 |
| analysis of xyloglucan endotransglycosylase/hydrolase (xth) genes from allotetraploid (gossypium hirsutum) cotton and its diploid progenitors expressed during fiber elongation. | multiple cellular pathways have been shown to be involved during fiber initiation and elongation stages in the cultivated allotetraploid cotton (gossypium hirsutum). the cell wall enzymes xyloglucan endotransglycosylase/hydrolases (xth) have been reported to be associated with the biosynthesis of the cell wall and the growth of cotton fibers, probably regulating the plasticity of the primary cell wall. among various cotton fiber cdnas found to be preferentially expressed in cotton fibers, a xylo ... | 2009 | 18789555 |
| genome-wide comparative analysis of nbs-encoding genes in four gossypium species. | nucleotide binding site (nbs) genes encode a large family of disease resistance (r) proteins in plants. the availability of genomic data of the two diploid cotton species, gossypium arboreum and gossypium raimondii, and the two allotetraploid cotton species, gossypium hirsutum (tm-1) and gossypium barbadense allow for a more comprehensive and systematic comparative study of nbs-encoding genes to elucidate the mechanisms of cotton disease resistance. | 2017 | 28403834 |
| characterization and expression analysis of terminal flower1 homologs from cultivated alloteraploid cotton (gossypium hirsutum) and its diploid progenitors. | the seasonal cycle and persistence of a plant is governed by a combination of the determinate or indeterminate status of shoot and root apical meristems. a perennial plant is one in which the apical meristem of at least one of its shoot axes remains indeterminate beyond the first growth season. terminal flower1 (tfl1) genes play important roles in regulating flowering time, the fate of inflorescence meristem and perenniality. to investigate the role of tfl1-like genes in the determination of the ... | 2008 | 18171595 |
| construction and analysis of cotton (gossypium arboreum l.) drought-related cdna library. | drought is one of the most important environmental factors causing water stress for cotton, and it greatly limits cotton growth and crop productivity. so far only a few drought-tolerance genes have been functionally characterized in details, and most efforts on this topic have been made in model organisms. therefore, to identify more drought-related genes in cotton plays a crucial role in elucidating the underlying mechanisms of drought tolerance as well as utilizing bioengineering techniques to ... | 2009 | 19570239 |
| co-expression network analyses identify functional modules associated with development and stress response in gossypium arboreum. | cotton is an economically important crop, essential for the agriculture and textile industries. through integrating transcriptomic data, we discovered that multi-dimensional co-expression network analysis was powerful for predicting cotton gene functions and functional modules. here, the recently available transcriptomic data on gossypium arboreum, including data on multiple growth stages of tissues and stress treatment samples were applied to construct a co-expression network exploring multi-di ... | 2016 | 27922095 |
| genome-wide characterization and expression profiles of the superoxide dismutase gene family in gossypium. | superoxide dismutase (sod) as a group of significant and ubiquitous enzymes plays a critical function in plant growth and development. previously this gene family has been investigated in arabidopsis and rice; it has not yet been characterized in cotton. in our study, it was the first time for us to perform a genome-wide analysis of sod gene family in cotton. our results showed that 10 genes of sod gene family were identified in gossypium arboreum and gossypium raimondii, including 6 cu-zn-sods, ... | 2016 | 27660755 |
| genetic diversity analysis of gossypium arboreum germplasm accessions using genotyping-by-sequencing. | the diploid cotton species gossypium arboreum possesses many favorable agronomic traits such as drought tolerance and disease resistance, which can be utilized in the development of improved upland cotton cultivars. the usda national plant germplasm system maintains more than 1600 g. arboreum accessions. little information is available on the genetic diversity of the collection thereby limiting the utilization of this cotton species. the genetic diversity and population structure of the g. arbor ... | 2016 | 27604991 |
| [identification and expression of six drought responsive transcripts through differential display in desi cottion (gossypium aroreum)]. | there is not enough information available on drought-modulated gene(s) in gossypium arboreum, which can be a valuable gene pool for improving modern cotton cultivars. in the present work differential display reverse transcriptase pcr (ddrt) was used to compare overall differences in gene expression between water stressed and control plants. by screening 93 primer-pair combinations ddrt technique resulted in up-regulation of 30 cdna transcripts. through reamplification and quality control assay 1 ... | 2008 | 18856054 |
| genome-wide comparison of ap2/erf superfamily genes between gossypium arboreum and g. raimondii. | the apetala2/ethylene response factor (ap2/erf) transcription factor superfamily is known to regulate diverse processes of plant development and stress responses. we conducted a genome-wide analysis of the ap2/erf gene in gossypium arboreum and g. raimondii. using rpsblast and hmmsearch, a total of 271 and 269 ap2/erf genes were identified in the g. arboreum and g. raimondii genomes, respectively. a phylogenetic analysis classified diploid gossypium spp ap2/erf genes into 4 families and 16 subfa ... | 2016 | 27525884 |
| genome-wide identification and comparative analysis of the 3-hydroxy-3-methylglutaryl coenzyme a reductase (hmgr) gene family in gossypium. | terpenes are the largest and most diverse class of secondary metabolites in plants and play a very important role in plant adaptation to environment. 3-hydroxy-3-methylglutaryl coenzyme a reductase (hmgr) is a rate-limiting enzyme in the process of terpene biosynthesis in the cytosol. previous study found the hmgr genes underwent gene expansion in gossypium raimondii, but the characteristics and evolution of the hmgr gene family in gossypium genus are unclear. in this study, genome-wide identifi ... | 2018 | 29364830 |
| genome-wide identification and characterization of spl transcription factor family and their evolution and expression profiling analysis in cotton. | plant specific transcription factors, squamosa promoter-binding protein-like (spl), are involved in many biological processes. however, no systematical study has been reported in cotton. in this study, a total of 177 spl genes were identified, including 29, 30, 59 and 59 spls in gossypium arboreum, g. raimondii, g. barbadense, and g. hirsutum, respectively. these spl genes were classified into eight phylogenetical groups. the gene structure, conserved motif, and clustering were highly conserved ... | 2018 | 29335584 |
| biochemical and molecular dissection of thermo-sensitive genetic male sterility in diploid cotton (gossypium arboreum l). | diploid cotton, due to its inherent problem of stamen brittleness, its found unsuitable for traditional method of hybrid seed production which involves hand emasculation followed by pollination. due to shortfall in other methods viz., genetic male sterility (gms), as well as, cytoplasmic genetic male sterility (cgms), hybrid seed production in diploid cotton becomes costly and thereby, covers less area among the total cotton grown area. thermo-sensitive genetic male sterility, which overcomes th ... | 2016 | 27498504 |
| comprehensive analysis of tcp transcription factors and their expression during cotton (gossypium arboreum) fiber early development. | tcp proteins are plant-specific transcription factors implicated to perform a variety of physiological functions during plant growth and development. in the current study, we performed for the first time the comprehensive analysis of tcp gene family in a diploid cotton species, gossypium arboreum, including phylogenetic analysis, chromosome location, gene duplication status, gene structure and conserved motif analysis, as well as expression profiles in fiber at different developmental stages. ou ... | 2016 | 26857372 |
| modeling of polysaccharide extraction from gossypium arboreum l. seed using central composite rotatable design. | the present study investigates the effect of independent variables such as extraction temperature (35-55 °c), time (1-5h) and solid-liquid ratio (1:5-1:25 g/ml) over the extraction yield of polysaccharide from gossypium arboreum l. seeds was investigated and optimized. aqueous extraction method was opted for the extraction of polysaccharide. central composite response surface design was utilized for developing the experimental design. a second order polynomial mathematical model was developed fr ... | 2016 | 26827756 |
| genome-wide analysis and expression profiling of the phospholipase d gene family in gossypium arboreum. | the plant phospholipase d (pld) plays versatile functions in multiple aspects of plant growth, development, and stress responses. however, until now, our knowledge concerning the pld gene family members and their expression patterns in cotton has been limited. in this study, we performed for the first time the genome-wide analysis and expression profiling of pld gene family in gossypium arboretum, and finally, a total of 19 non-redundant pld genes (gaplds) were identified. based on the phylogene ... | 2016 | 26718354 |
| assessing genetic diversity among six populations of gossypium arboreum l. using microsatellites markers. | among the four cultivated cotton species, g. hirsutum (allotetraploid) presently holds a primary place in cultivation. efforts to further improve this primary cotton face the constraints of its narrow genetic base due to repeated selective breeding and hence demands enrichment of diversity in the gene pool. g. arboreum (diploid species) is an invaluable genetic resource with great potential in this direction. based on the dispersal and domestication in different directions from indus valley, dif ... | 2015 | 26600679 |
| a global assembly of cotton ests. | approximately 185,000 gossypium est sequences comprising >94,800,000 nucleotides were amassed from 30 cdna libraries constructed from a variety of tissues and organs under a range of conditions, including drought stress and pathogen challenges. these libraries were derived from allopolyploid cotton (gossypium hirsutum; a(t) and d(t) genomes) as well as its two diploid progenitors, gossypium arboreum (a genome) and gossypium raimondii (d genome). ests were assembled using the program for assembli ... | 2006 | 16478941 |
| genome sequence of the cultivated cotton gossypium arboreum. | the complex allotetraploid nature of the cotton genome (aadd; 2n = 52) makes genetic, genomic and functional analyses extremely challenging. here we sequenced and assembled the gossypium arboreum (aa; 2n = 26) genome, a putative contributor of the a subgenome. a total of 193.6 gb of clean sequence covering the genome by 112.6-fold was obtained by paired-end sequencing. we further anchored and oriented 90.4% of the assembly on 13 pseudochromosomes and found that 68.5% of the genome is occupied by ... | 2014 | 24836287 |
| microcolinearity and genome evolution in the adha region of diploid and polyploid cotton (gossypium). | genome sizes vary by several orders of magnitude, driven by mechanisms such as illegitimate recombination and transposable element proliferation. prior analysis of the cesa region in two cotton genomes that diverged 5-10 million years ago (ma), and acquired a twofold difference in genome size, revealed extensive local conservation of genic and intergenic regions, with no evidence of the global genome size difference. the present study extends the comparison to include bac sequences surrounding t ... | 2007 | 17461788 |
| genetic diversity and relationship analysis of gossypium arboreum accessions. | simple sequence repeat techniques were used to identify the genetic diversity of 101 gossypium arboreum accessions collected from india, vietnam, and the southwest of china (guizhou, guangxi, and yunnan provinces). twenty-six pairs of ssr primers produced a total of 103 polymorphic loci with an average of 3.96 polymorphic loci per primer. the average of the effective number of alleles, nei's gene diversity, and shannon's information index were 0.59, 0.2835, and 0.4361, respectively. the diversit ... | 2015 | 26600511 |
| development of chromosome-specific markers with high polymorphism for allotetraploid cotton based on genome-wide characterization of simple sequence repeats in diploid cottons (gossypium arboreum l. and gossypium raimondii ulbrich). | tetraploid cotton contains two sets of homologous chromosomes, the at- and dt-subgenomes. consequently, many markers in cotton were mapped to multiple positions during linkage genetic map construction, posing a challenge to anchoring linkage groups and mapping economically-important genes to particular chromosomes. chromosome-specific markers could solve this problem. recently, the genomes of two diploid species were sequenced whose progenitors were putative contributors of the at- and dt-subgen ... | 2015 | 25652321 |
| genome-wide investigation and transcriptome analysis of the wrky gene family in gossypium. | wrky transcription factors play important roles in various stress responses in diverse plant species. in cotton, this family has not been well studied, especially in relation to fiber development. here, the genomes and transcriptomes of gossypium raimondii and gossypium arboreum were investigated to identify fiber development related wrky genes. this represents the first comprehensive comparative study of wrky transcription factors in both diploid a and d cotton species. in total, 112 g. raimond ... | 2015 | 25190108 |
| isolation and functional characterization of a τ-cadinol synthase, a new sesquiterpene synthase from lavandula angustifolia. | in this paper we characterize three stpss: a germacrene d (lagerds), a (e)-β-caryophyllene (lacars) and a τ-cadinol synthase (lacads). τ-cadinol synthase is reported here for the first time and its activity was studied in several biological models including transiently or stably transformed tobacco species. three dimensional structure models of lacads and ocimum basilicum γ-cadinene synthase were built by homology modeling using the template structure of gossypium arboreum δ-cadinene synthase. t ... | 2014 | 24078339 |
| induction of cotton ovule culture fibre branching by co-expression of cotton btl, cotton sim, and arabidopsis sti genes. | the highly elongated single-celled cotton fibre consists of lint and fuzz, similar to the arabidopsis trichome. endoreduplication is an important determinant in arabidopsis trichome initiation and morphogenesis. fibre development is also controlled by functional homologues of arabidopsis trichome patterning genes, although fibre cells do not have a branched shape like trichomes. the identification and characterization of the homologues of 10 key arabidopsis trichome branching genes in gossypium ... | 2013 | 23966592 |
| comparative proteomic analysis reveals differentially expressed proteins correlated with fuzz fiber initiation in diploid cotton (gossypium arboreum l.). | in this study, a comparative proteomic analysis was employed to identify fuzz fiber initiation-related proteins in wild-type diploid cotton (gossypium arboreum l.) and its fuzzless mutant. temporal changes in global proteomes were examined using 2-de at five developmental time points for fuzz fiber initiation, and 71 differentially expressed protein species were identified by ms, 45 of which were preferentially accumulated in the wild-type. these proteins were assigned to several functional cate ... | 2013 | 23474080 |
| mrna-seq analysis of the gossypium arboreum transcriptome reveals tissue selective signaling in response to water stress during seedling stage. | the cotton diploid species, gossypium arboreum, shows important properties of stress tolerance and good genetic stability. in this study, through mrna-seq, we de novo assembled the unigenes of multiple samples with 3h h(2)o, nacl, or peg treatments in leaf, stem and root tissues and successfully obtained 123,579 transcripts of g. arboreum, 89,128 of which were with hits through blast against known cotton ests and draft genome of g. raimondii. about 36,961 transcripts (including 1,958 possible tr ... | 2013 | 23382961 |
| identification and characterization of micrornas in asiatic cotton (gossypium arboreum l.). | to date, no mirnas have been identified in the important diploid cotton species although there are several reports on mirnas in upland cotton. in this study, we identified 73 mirnas, belonging to 49 families, from asiatic cotton using a well-developed comparative genome-based homologue search. several of the predicted mirnas were validated using quantitative real time pcr (qrt-pcr). the length of mirnas varied from 18 to 22 nt with an average of 20 nt. the length of mirna precursors also varied ... | 2012 | 22493671 |