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plant compounds that induce polychlorinated biphenyl biodegradation by arthrobacter sp. strain b1b.plant compounds that induced arthrobacter sp. strain b1b to cometabolize polychlorinated biphenyls (pcbs) were identified by a screening assay based on the formation of a 4,4'-dichlorobiphenyl ring fission product. a chemical component of spearmint (mentha spicata), l-carvone, induced arthrobacter sp. strain b1b to cometabolize aroclor 1242, resulting in significant degradation of 26 peaks in the mixture, including selected tetra- and pentachlorobiphenyls. evidence for pcb biodegradation include ...19979143124
heteromeric geranyl diphosphate synthase from mint: construction of a functional fusion protein and inhibition by bisphosphonate substrate analogs.geranyl diphosphate synthase catalyzes the condensation of dimethylallyl diphosphate (c(5)) with isopentenyl diphosphate (c(5)) to produce geranyl diphosphate (c(10)), the essential precursor of monoterpenes. the enzyme from peppermint and spearmint (menthaxpiperita and mentha spicata, respectively) functions as a heterodimer or heterotetramer consisting of a 40kda subunit and 33kda subunit. the dnas encoding each subunit were joined with different sized linkers and in both possible orders, and ...200414725857
effects of peppermint teas on plasma testosterone, follicle-stimulating hormone, and luteinizing hormone levels and testicular tissue in rats.to justify the effects of mentha piperita labiatae and mentha spicata labiatae herbal teas on plasma total testosterone, luteinizing hormone, and follicle-stimulating hormone levels and testicular histologic features. we performed this study because of major complaints in our area from men about the adverse effects of these herbs on male reproductive function.200415302514
comparative screening of plant essential oils: phenylpropanoid moiety as basic core for antiplatelet activity.essential oils extracted from different plants (anthemis nobilis l., artemisia dracunculus l., cannabis sativa l., cupressus sempervirens l., cymbopogon citratus (dc.) stapf., curcuma longa l., foeniculum vulgare l., hypericum perforatum l., hyssopus officinalis l., mentha spicata l., monarda didyma l., ocimum basilicum l., ocotea quixos kosterm., origanum vulgare l., pinus nigra j.f. arnold, pinus silvestris l., piper crassinervium kunth., rosmarinus officinalis l., salvia officinalis l., salvi ...200616274702
metabolic engineering of monoterpene biosynthesis: two-step production of (+)-trans-isopiperitenol by tobacco.monoterpenoid biosynthesis in tobacco was modified by introducing two subsequent enzymatic activities targeted to different cell compartments. a limonene-3-hydroxylase (lim3h) cdna was isolated from mentha spicata l. 'crispa'. this cdna was used to re-transform a transgenic nicotiana tabacum'petit havana' sr1 (tobacco) line expressing three citrus limon l. burm. f. (lemon) monoterpene synthases producing (+)-limonene, gamma-terpinene and (-)-beta-pinene as their main products. the targeting sequ ...200415200648
investigation of biochemical and histopathological effects of mentha piperita labiatae and mentha spicata labiatae on liver tissue in rats.the plant mentha piperita, or peppermint, is commonly used in the treatment of loss of appetite, common cold, bronchitis, sinusitis, fever, nausea and vomiting, and indigestion as a herbal agent. in this study, we aimed to investigate biochemical and histological effects of m. piperita labiatae, growing in the yenisar bademli town of isparta city, and mentha spicata labiatae, growing in the anamas high plateau of the yenisar bademli town, on the rat liver tissue. forty-eight male wistar albino r ...200415027812
investigation of biochemical and histopathological effects of mentha piperita l. and mentha spicata l. on kidney tissue in rats.peppermint plants have been used as a herbal medicine for many conditions, including loss of appetite, common cold, bronchitis, sinusitis, fever, nausea, vomiting and indigestion. this study is aimed at investigating the biochemical and histological effects of mentha piperita l., growing in the yenisar bademli town of isparta city, and mentha spicata l., growing on the anamas high plateau of isparta city, on rat kidney tissue. forty-eight male wistar albino rats weighing 200-250 g were used for ...200312755472
induction and characterization of a cytochrome p-450-dependent camphor hydroxylase in tissue cultures of common sage (salvia officinalis).(+)-camphor, a major monoterpene of the essential oil of common sage (salvia officinalis), is catabolized in senescent tissue, and the pathway for the breakdown of this bicyclic ketone has been previously elucidated in sage cell-suspension cultures. in the initial step of catabolism, camphor is oxidized to 6-exo-hydroxycamphor, and the corresponding nadph- and o2-dependent hydroxylase activity was demonstrated in microsomal preparations of sage cells. several well-established inhibitors of cytoc ...199312231778
inhibition by the essential oils of peppermint and spearmint of the growth of pathogenic bacteria.the effects of the, essential oils of peppermint (mentha piperita l.), spearmint mentha spicata l.) and japanese mint (mentha, arvensis l.), of four major constituents of the esssential oil of peppermint, and of three major constituents of the essential oil of spearmint, on the proliferation of helicobacter pylori, salmonella enteritidis, escherichia coli o157:h7, methicillin-resistant staphylococcus aureus (mrsa) and methicillin sensitive staphylococccus aureus (mssa) were examined. the essent ...200111549238
(3r)-linalool synthase from artemisia annua l.: cdna isolation, characterization, and wound induction.artemisia annua is an annual herb used in traditional chinese medicine. a cdna library was constructed from leaves of a. annua seedlings and target sequences were amplified by pcr using degenerate primers derived from a consensus sequence of angiosperm terpene synthases. two clones, qh1 and qh5, with high sequence similarity to plant monoterpene synthases were ultimately obtained and expressed in escherichia coli. these cdnas encode peptides of 567 aa (65.7 kda) and 583 aa (67.4 kda), respective ...199910562427
purification and characterization of an nadph-cytochrome p450 (cytochrome c) reductase from spearmint (mentha spicata) glandular trichomes.solubilized nadph-cytochrome c (p450) reductase was purified to homogeneity from an extract of spearmint (mentha spicata) glandular trichomes by dye-ligand interaction chromatography on matrex-gel red a and affinity chromatography on 2', 5'-adenosine diphosphate agarose. sds-page of the purified enzyme preparation revealed the presence of two similar proteins with masses of 82 kda (major) and 77 kda (minor) that crossreacted on immunoblot analysis with polyclonal antibodies directed against nadp ...19968619640
production and characterization of polyclonal antibodies in rabbits to 4s-limonene synthase from spearmint (mentha spicata).limonene synthase, a monoterpene cyclase from the oil glands of spearmint (mentha spicata) leaves that catalyzes the conversion of geranyl pyrophosphate to (-)-4s-limonene, was purified, and polyclonal antibodies were generated in rabbits against the sodium dodecyl sulfate-denatured protein. immunoblotting analysis revealed that the antibodies were very specific for denatured limonene synthase from all mentha species tested. however, no immunological cross-reactivity was observed with denatured ...19938442666
monoterpene synthases of pinus contorta and related conifers. a new class of terpenoid cyclase.a cell-free extract from the xylem of lodgepole pine (pinus contorta) catalyzes the conversion of [1-3h1]geranyl pyrophosphate to a variety of monoterpene olefins found in lodgepole pine oleoresin. this monoterpene synthase activity is similar to previously described terpenoid cyclases from grand fir (abies grandis) and other higher plants in molecular mass (67 +/- 2 kda as estimated by size-exclusion chromatography), km for geranyl pyrophosphate (7.8 +/- 1.9 microm), and isoelectric point (4.75 ...19948307957
4s-limonene synthase from the oil glands of spearmint (mentha spicata). cdna isolation, characterization, and bacterial expression of the catalytically active monoterpene cyclase.the committed step in the biosynthesis of monoterpenes in mint (mentha) species is the cyclization of geranyl pyrophosphate to the olefin (-)-4s-limonene catalyzed by limonene synthase (cyclase). internal amino acid sequences of the purified enzyme from spearmint oil glands were utilized to design three distinct oligonucleotide probes. these probes were subsequently employed to screen a spearmint leaf cdna library, and four clones were isolated. three of these cdna isolates were full-length and ...19938226816
expression of spearmint limonene synthase in transgenic spike lavender results in an altered monoterpene composition in developing leaves.we generated transgenic spike lavender (lavandula latifolia) plants constitutively expressing the limonene synthase (ls) gene from spearmint (mentha spicata), encoding the ls enzyme that catalyzes the synthesis of limonene from geranyl diphosphate. overexpression of the ls transgene did not consistently affect monoterpene profile in pooled leaves or flowers from transgenic t(0) plants. analyses from cohorts of leaves sampled at different developmental stages showed that essential oil accumulatio ...200818514005
antinociceptive activity of (-)-carvone: evidence of association with decreased peripheral nerve excitability.(-)-carvone is a monoterpene ketone that is the main active component of mentha plant species like mentha spicata. this study aimed to investigate the antinociceptive activity of (-)-carvone using different experimental models of pain and to investigate whether such effects might be involved in the nervous excitability elicited by others monoterpenes. in the acetic acid-induced writhing test, we observed that (-)-carvone-treated mice exhibited a significant decrease in the number of writhes when ...200818451538
toxicity of plant essential oils and their components against lycoriella ingenua (diptera: sciaridae).plant essential oils from 20 plant species were tested for their insecticidal activity against larvae of lycoriella ingenua (dufour) (diptera: sciaridae) by using a fumigation bioassay. good insecticidal activity (>90%) against larvae of l. ingenua was achieved with essential oils of caraway seed carum carvi (l.)], lemongrass [cymbopogon citratus (d.c.) stapf.], mandarine (citrus reticulate blanco), nutmeg (myristica fragrans houtt), cade (juniperus oxycedrus l.), spearmint (mentha spicata l.), ...200818330128
iron and magnesium concentrations of mint accessions (mentha spp.).plant foods can contribute significantly to human nutrition and health, because they contain almost all essential human nutrients. however, nutrient composition varies among different plant foods. improvement of nutritional quality of our food supply, especially with respect to essential nutrient minerals, such as magnesium, iron and zinc, could be an important goal of vegetable crops. there is little information available on essential mineral concentration of mint (mentha spp.). this study was ...200717468000
effect of spearmint (mentha spicata labiatae) teas on androgen levels in women with hirsutism.mentha spicata labiatae, known as spearmint and mentha piperita labiatae, known as peppermint can be used for various kinds of illnesses in herbal medicine and flavoring in industry. m. spicata labiatae grows on the anamas plateau of yenithornarbademli town of isparta, located in southwest part of turkey. in this town, clinicians thought that consumption of tea steeped with m. spicata or m. piperita caused a diminished libido. because antiandrogenic effects of spearmint and peppermint were found ...200717310494
comparison of essential oils from three plants for enhancement of antimicrobial activity of nitrofurantoin against enterobacteria.piperitone from plant essential oils enhancesbactericidal activities of nitrofurantoin and furazolidone against bacteria from the family enterobacteriaceae. in this study, the essential oils of spearmint (mentha spicata l.), dill (anethum graveolens l.) and peppermint (mentha piperita l.)were screened for augmentation of nitrofurantoin activity and the most active components were determined.200717192709
protective effects of solvent fractions of mentha spicata (l.) leaves evaluated on 4-nitroquinoline-1-oxide induced chromosome damage and apoptosis in mouse bone marrow cells.spearmint leaves (mentha spicata l.) contain high levels of antioxidants that are known to protect against both exogenous and endogenous dna damage. in this study, the protective effects of the hexane fraction (hf), chloroform fraction (cf) and ethyl acetate fraction (eaf) in an ethanol extract from m. spicata were evaluated against 4-nitroquinoline-1-oxide (4-nqo) induced chromosome damage and apoptosis in bone marrow cells of swiss albino mice. two (eaf; 80 and 160 mg/ kg body weight - bw) or ...200921637463
the effect of mentha spicata labiatae on uterine tissue in rats.the plant mentha spicata, or peppermint, is commonly used in the treatment of loss of appetite, common cold, bronchitis, sinusitis, fever, nausea and vomiting, and indigestion as a herbal agent. in this study, we aimed to investigate the biochemical and histological effects of m. spicata labiatae, growing on the anamas high plateau of yenisarbademli town, on rat uterine tissue. twenty female wistar albino rats weighing 160+/-10 g were used for this study. rats were divided into two groups of ten ...200617120533
correlation between the in vitro antioxidant activity and polyphenol content of aqueous extracts from bulgarian herbs.the water phase antioxidant activity of extracts from 23 bulgarian medicinal plants was studied in relation to their polyphenol content in comparison with mate, black tea, honeybush and rooibos foreign species. antioxidant activity was measured by the abts (2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)) cation radical decolorization assay, and the total polyphenol content was assayed according to the folin-ciocalteu method. five bulgarian plant extracts exhibited higher antioxidant activ ...200616906640
bactericidal activity of organic extracts from flourensia cernua dc against strains of mycobacterium tuberculosis.tuberculosis is a chronic disease caused mainly by mycobacterium tuberculosis. the emergence of antibiotic-resistant strains of this species underscores the need for novel effective drugs against resistant mycobacteria as first-line antituberculosis medications.200616314185
screening of plants used in danish folk medicine to treat memory dysfunction for acetylcholinesterase inhibitory activity.aqueous and methanolic extracts of 11 plants, used in danish folk medicine for improvement of memory and cognition, and 3 corydalis species were tested for acetylcholinesterase inhibitory activity using the ellman colorimetric method. significant inhibitory activity in dose-dependent manner was observed for extracts of corydalis cava, corydalis intermedia, corydalis solida ssp. laxa and corydalis solida ssp. slivenensis. extracts of ruta graveolens, lavandula angustifolia, rosmarinus officinalis ...200616280217
Chemical composition and larvicidal activity of essential oil from Mentha spicata (Linn.) against three mosquito species.Mosquitoes are blood-feeding insects and serve as the most important vectors for spreading human diseases such as malaria, yellow fever, dengue fever, and filariasis. The continued use of synthetic insecticides has resulted in resistance in mosquitoes. Synthetic insecticides are toxic and affect the environment by contaminating soil, water, and air, and then natural products may be an alternative to synthetic insecticides because they are effective, biodegradable, eco-friendly, and safe to envir ...201122139403
influence of autoclaved fungal materials on spearmint (mentha spicata l.) growth, morphogenesis, and secondary metabolism.the influence of autoclaved fungal materials such as culture filtrate, freeze-dried mycelium (fdm), mycelium suspension, and spore suspension (ss) on the growth, morphogenesis, and carvone production of spearmint (mentha spicata l.) plants was studied. fungal materials were either applied as a drench or spray on the plants. spearmint plants (cv. "294099") drenched with ss (1 x 10(8) spores/ml) of trichoderma reesei showed no significant differences in leaf numbers, root numbers, or shoot numbers ...200516222794
effect of mentha piperita (labiatae) and mentha spicata (labiatae) on iron absorption in rats.the effect of mentha piperita (labiatae) and mentha spicata (labiatae) teas, which contain different phenol compounds, on iron metabolism was studied. these teas grow in different areas of the city of isparta, turkey. these herbals were given to the rats in tea.200415941008
evaluation of phenolic compounds and antioxidant and antimicrobial activities of some common herbs.the study was designed to evaluate the phenolic, flavonoid contents and antioxidant and antimicrobial activities of onion (allium cepa), garlic (allium sativum), mint (mentha spicata), thyme (thymus vulgaris), oak (quercus), aloe vera (aloe barbadensis miller), and ginger (zingiber officinale). all extracts showed a wide range of total phenolic contents, that is, 4.96 to 98.37 mg/100 g gallic acid equivalents, and total flavonoid contents, that is, 0.41 to 17.64 mg/100 g catechin equivalents. an ...201728316626
monoterpene metabolism. cloning, expression, and characterization of (-)-isopiperitenol/(-)-carveol dehydrogenase of peppermint and spearmint.the essential oils of peppermint (mentha x piperita) and spearmint (mentha spicata) are distinguished by the oxygenation position on the p-menthane ring of the constitutive monoterpenes that is conferred by two regiospecific cytochrome p450 limonene-3- and limonene-6-hydroxylases. following hydroxylation of limonene, an apparently similar dehydrogenase oxidizes (-)-trans-isopiperitenol to (-)-isopiperitenone in peppermint and (-)-trans-carveol to (-)-carvone in spearmint. random sequencing of a ...200515734920
antimutagenic activity of spearmint.the antimutagenic activity of spearmint (mentha spicata), a popular food flavoring agent, was studied in the salmonella assay. spearmint leaves were brewed in hot water for 5 min at concentrations up to 5% (w/v), and the water extracts were tested against the direct-acting mutagens 4-nitro-1,2-phenylenediamine (npd) and 2-hydroxyamino-3-methyl-3h-imidazo[4,5-f]quinoline (n-oh-iq) using salmonella typhimurium strain ta98. nontoxic concentrations of spearmint extract inhibited the mutagenic activi ...200415529323
[diversity of phytophagous and pathogens and their damage to mints (mentha)].the aim of this work was to estimate the phytopathologic condition of mints grown and self-growing in kaunas botanical garden of vytautas magnus university and elsewhere in lithuania; and to identify the specific constitution, the spread and damage of the main pests and disease agents. material and methods: observations and examination were carried out with two local, four introduced mint species and with four hybrids in 1997-2003. we estimated the plant condition by identifying the intensity of ...200415299999
spearmint induced hypothalamic oxidative stress and testicular anti-androgenicity in male rats - altered levels of gene expression, enzymes and hormones.mentha spicata labiatae, commonly known as spearmint, can be used for various kinds of illnesses in herbal medicines and food industries. one of the prominent functions of this plant extract is its anti-androgenic activity. the present study investigated the probable correlation between oxidative stress in hypothalamic region and anti-androgenic action of this plant's aqueous extract on rats. decreased activities of enzymes like superoxide dismutase, catalase, glutathione peroxidase and glutathi ...200818804513
mint oil (mentha spicata linn.) offers behavioral radioprotection: a radiation-induced conditioned taste aversion study.mentha spicata linn. (mint), a herb well known for its gastroprotective properties in the traditional system of medicine has been shown to protect against radiation-induced lethality, and recently its constituents have been found to possess calcium channel antagonizing properties. the present study examined the behavioral radioprotective efficacy of mint oil (obtained from mentha spicata), particularly in mitigating radiation-induced conditioned taste aversion (cta), which has been proposed as a ...200918853399
chemical composition of essential oils of thymus and mentha species and their antifungal activities.the potential antifungal effects of thymus vulgaris l., thymus tosevii l., mentha spicata l., and mentha piperita l. (labiatae) essential oils and their components against 17 micromycetal food poisoning, plant, animal and human pathogens are presented. the essential oils were obtained by hydrodestillation of dried plant material. their composition was determined by gc-ms. identification of individual constituents was made by comparison with analytical standards, and by computer matching mass spe ...200919136911
distribution, feeding behavior and control strategies of the exotic land snail achatina fulica (gastropoda: pulmonata) in the northeast of brazil.the goal of this study was to document the distribution and establishment a. fulica such as their feeding preference and behavior in situ. the study was carried out at the city of lauro de freitas, bahia state, brazil, between november 2001 and november 2002. we used catch per unit effort methods to determine abundance, distribution, habitat choice and food preferences. the abundance and distribution of a. fulica was most representative in urban area, mainly near to the coastline. lots and house ...200819197503
the effect of mentha spicata and eucalyptus camaldulensis essential oils on dental biofilm.to assess the antimicrobial effects of mentha spicata and eucalyptus camaldulensis essential oils and chlorhexidine against streptococcus mutans and streptococcus pyogenes, with a particular focus on in vitro and in vivo biofilm formation.200919659716
antigenotoxic and antioxidant potential of aqueous fraction of ethanol extract of mentha spicata (l.) against 4-nitroquinoline-1-oxide-induced chromosome damage in mice.the antigenotoxic potential of an aqueous fraction of ethanol extract of mentha spicata was evaluated by measuring the frequency of micronucleated polychromatic erythrocytes (mnpces) in mice bone marrow, using 4-nitroquinoline-1oxide (nqo) as the reference mutagen. in addition, lipid peroxidation (lpo) and antioxidant levels were also quantified with liver tissue of the same mice to assess their antioxidant potential. swiss albino mice of either sex (25-30 g) were orally pretreated with an aqueo ...200919793034
ethnopharmacy of turkish-speaking cypriots in greater london.for centuries, in the eastern mediterranean region, medicinal plant use has been widely accepted as a treatment method for both minor and major diseases. although some knowledge exists on the use of such medicinal plants within the greek cypriot culture and considerable information is available on various regions in turkey, no detailed ethnopharmaceutical or ethnobotanical studies exist on turkish-speaking cypriots (tsc) both in cyprus and within one of the largest tsc migrant communities in lon ...201019827023
ethnobotanical study of medicinal plants from the humla district of western nepal.the present paper documents the uses of plants in traditional herbal medicine for treatment of human and veterinary ailments in four village development committees in the humla district of western nepal. it also determines the homogeneity of informant's knowledge on medicinal plants suitable for different ailment categories and the most preferred plant species used to treat each ailment category in the study areas.201020553834
activities of ten essential oils towards propionibacterium acnes and pc-3, a-549 and mcf-7 cancer cells.ten essential oils, namely, mint (mentha spicata l., lamiaceae), ginger (zingiber officinale rosc., zingiberaceae), lemon (citrus limon burm.f., rutaceae), grapefruit (citrus paradisi macf., rutaceae), jasmine (jasminum grandiflora l., oleaceae), lavender (mill., lamiaceae), chamomile (matricaria chamomilla l., compositae), thyme (thymus vulgaris l., lamiaceae), rose (rosa damascena mill., rosaceae) and cinnamon (cinnamomum zeylanicum n. lauraceae) were tested for their antibacterial activities ...201020657472
an ethnobotanical survey of medicinal plants in sivrice (elazığ-turkey).this study aimed to identify wild plants collected for medical purposes by the local people of sivrice county, located in the eastern anatolia region of turkey, and to establish the uses and local names of these plants.201020713142
yield, content, and composition of peppermint and spearmints as a function of harvesting time and drying.peppermint ( mentha × piperita l.) and spearmints ('scotch' spearmint, m. × gracilis sole, and 'native' spearmint, mentha spicata l.) are widely grown essential oil crops in more northern latitudes; however, there is limited information on how harvest time and drying influence peppermint and spearmint yield, oil composition, and bioactivity, when grown south of the 41st parallel. in this 2-year study, the effects of harvest time and drying on the yield, oil composition, and bioactivity of pepper ...201020942459
analysis and evaluation of antibacterial effects of new herbal formulas, ap-001 and ap-002, against escherichia coli o157:h7.over the last few years, there has been an increasing interest in the use of natural substances for promoting human and animal health. antibiotic resistance, enhanced by excessive use of antibiotics, has encouraged human and animal health authorities to consider and employ alternative approaches, including herbal and botanical medicine to combat invading microorganisms. herein, the essential oils of thymus vulgaris, allium cepa, allium sativum, eucalyptus globulus, salvia officinalis, dianthus c ...201526032257
antimicrobial activity of plant essential oils against bacterial and fungal species involved in food poisoning and/or food decay.the currative properties of aromatic and medicinal plants have been recognized since ancient times and, more recently, the antimicrobial activity of plant essential oils has been used in several applications, including food preservation. the purpose of this study was to create directly comparable, quantitative data on the antimicrobial activity of some plant essential oils prepared in the national institute of research-development for chemistry and petrochemistry, bucharest to be used for the fu ...201021462837
free radical scavenging (dpph) potential in nine mentha species.mentha species are used in every day life in various food items. these species produce valuable secondary metabolites that scavenge toxic free radicals. toxic free radicals can cause different diseases in the human body. in the present study free radical scavenging potential (1,1-diphenyl-2-picrylhydrazyl scavenging activity) in nine mentha species were investigated to evaluate and explore new potential sources for natural antioxidants. the activity was performed after different time intervals w ...201121646282
ethnopharmacological survey of medicinal plants in maden (elazig-turkey).this study aimed to identify wild plants collected for medical purposes by the local people of maden county, located in the eastern anatolia region of turkey, and to establish the uses and local names of these plants.201121704144
medicinal plants as antiemetics in the treatment of cancer: a review.chemotherapy- and radiotherapy-induced nausea and vomiting are the most common, intractable and unpleasant side effects in patients undergoing treatment for cancer. 5-hydroxytryptamine-3 (5-ht3) receptor antagonists plus dexamethasone have significantly improved the control of acute nausea and vomiting, but delayed nausea and vomiting remains a significant clinical problem. combined neurokinin-1 receptor antagonists with 5-ht3 antagonists and steroids are observed to be better in the control of ...201121821652
anti-inflammatory activity of four solvent fractions of ethanol extract of mentha spicata l. investigated on acute and chronic inflammation induced rats.anti-inflammatory effects of four solvent fractions of ethanol extract of mentha spicata were evaluated in acute and chronic inflammation induced in wistar albino rats. lipid peroxidation (lpo) and some antioxidants produced during chronic inflammation were quantitated. hexane (320mg/kg of body weight in 25% dmso), chloroform (320mg/kg body weight in 25% dmso), ethyl acetate (160mg/kg body weight in 25% dmso), aqueous (320mg/kg of body weight in ddh(2)o) fractions, two negative control groups (2 ...200821783894
repellent activities of some labiatae plant essential oils against the saltmarsh mosquito ochlerotatus caspius (pallas, 1771) (diptera: culicidae).the repellent activities of the essential oils of two thymus (thymus sipyleus boiss. subsp. sipyleus and thymus revolutus celak) and two mentha (mentha spicata l. subsp. spicata and mentha longifolia l.) species against ochlerotatus caspius (pallas, 1771) (diptera: culicidae) are presented. the essential oils were obtained by hydrodistillation of the aerial parts of the plants in flowering period and repellency tests were done with a y-tube olfactometer. all essential oils showed repellency in v ...201122179264
xanthine oxidase inhibitory activity of the methanolic extracts of selected jordanian medicinal plants.the search for novel xanthine oxidase (xo) inhibitors with a higher therapeutic activity and fewer side effects are desired not only to treat gout but also to combat various other diseases associated with the xo activity. at present, the potential of developing successful natural products for the management of xo-related diseases is still largely unexplored. in the present study, we have screened the methanolic extracts of various jordanian medicinal plants for their xo inhibitory activities usi ...201122262935
efficacy of mentha spicata essential oil in suppression of aspergillus flavus and aflatoxin contamination in chickpea with particular emphasis to mode of antifungal action.the present study reports in vivo antifungal and antiaflatoxigenic efficacy of mentha spicata essential oil (eo) against toxigenic aspergillus flavus strain lhp(c)-d6 in chickpea food system up to 12 months of storage. in addition, the mode of antifungal action of eo was also determined to understand the mechanism of fungal growth inhibition. the in vivo study with different concentrations of m. spicata eo showed dose-dependent decrease in fungal colony count as well as aflatoxin b1 concentratio ...201626338202
(-)-carvone: antispasmodic effect and mode of action.(-)-carvone is a monoterpene ketone found in spearmint (mentha spicata var. crispa) essential oil that is widely used as an odor and flavor additive. an intestinal antispasmodic effect was recently reported for (-)-carvone, and it has been shown to be more potent than its (+)-antipode. the mechanism of (-)-carvone action in the intestines has not been investigated. to gain a better understanding of the (-)-carvone antispasmodic effect, we investigated its pharmacological effects in the guinea pi ...201323103297
phytochemical profile and evaluation of the biological activities of essential oils derived from the greek aromatic plant species ocimum basilicum, mentha spicata, pimpinella anisum and fortunella margarita.natural products, known for their medicinal properties since antiquity, are continuously being studied for their biological properties. in the present study, we analyzed the composition of the volatile preparations of essential oils of the greek plants ocimum basilicum (sweet basil), mentha spicata (spearmint), pimpinella anisum (anise) and fortunella margarita (kumquat). gc/ms analyses revealed that the major components in the essential oil fractions, were carvone (85.4%) in spearmint, methyl c ...201627537869
in vitro study of the antifungal activity of essential oils obtained from mentha spicata, thymus vulgaris and laurus nobilis.the aim of this study was to determine the antifungal activity of the essential oils isolated from three aromatic plants against 13 filamentous fungal strains. the major constituents of mentha spicata, thymus vulgaris, and laurus nobilis essential oils were carvone (52.2%), linalool (78.1%), and 1,8-cineole (45.6%), respectively. m. spicata essential oil exhibited the strongest activity against all tested fungi in which fusarium graminearum, f.moniliforme, and penicillium expansum were the most ...201627697030
a survey of insect assemblages responding to volatiles from a ubiquitous fungus in an agricultural landscape.we report here a first survey of insect orientation to fungal cultures and fungal volatiles from a community ecology perspective. we tested whether volatiles from a ubiquitous yeast-like fungus (aureobasidium pullulans) are broadly attractive to insects in an agricultural landscape. we evaluated insect attraction to fungal cultures and synthetic compounds identified in fungal headspace (2-methyl-1-butanol, 3-methyl-1-butanol, 2-phenylethanol) in a spearmint (mentha spicata l.) plantation. three ...201323564294
chemical composition and in vitro antibacterial activity of mentha spicata essential oil against common food-borne pathogenic bacteria.the aim of the present study was to investigate chemical composition and antibacterial activity of essential oil from the leaf of mentha spicata plant against common food-borne pathogenic bacteria (staphylococcus aureus, bacillus subtilis, bacillus cereus, listeria monocytogenes, salmonella typhimurium, and escherichia coli o157:h7). chemical composition of the essential oil was identified by gas chromatography coupled with mass spectrometer detector (gc-ms). the antibacterial activity of the es ...201526351584
green synthesis, characterisation and biological evaluation of agnps using agave americana, mentha spicata and mangifera indica aqueous leaves extract.the current study was performed to synthesize stable, eco-friendly and bio-compatible silver nano-particles (agnps) of agave americana, mentha spicata and mangifera indica leaves and to screen them for biological activities. the ultraviolet-visible spectroscopic analysis revealed that λ-max for agnps range from 350-500 nm. all agnps possessed polycrystalline structure as notified as intense graphical peaks in complete spectrum of 20 values ranging from 10-80° in x-ray diffraction measurements an ...201627676375
efficacy of medicinal essential oils against pathogenic malassezia sp. isolates.the purposes of this study were to evaluate the distribution pattern and population size of malassezia species in dogs with atopic dermatitis (ad) and the inhibitory efficacy of zataria multiflora, thymus kotschyanus, mentha spicata, artemisia sieberi, rosmarinus officinalis and heracleum persicum essential oils against pathogenic malassezia isolates.201626597143
nematicidal activity of mint aqueous extracts against the root-knot nematode meloidogyne incognita.the nematicidal activity and chemical characterization of aqueous extracts and essential oils of three mint species, namely, mentha × piperita , mentha spicata , and mentha pulegium , were investigated. the phytochemical analysis of the essential oils was performed by means of gc-ms, whereas the aqueous extracts were analyzed by lc-ms. the most abundant terpenes were isomenthone, menthone, menthol, pulegone, and carvone, and the water extracts yielded mainly chlorogenic acid, salvianolic acid b, ...201324050256
mint essential oil can induce or inhibit potato sprouting by differential alteration of apical meristem.sprouting of potatoes during storage, due to tuber dormancy release, is associated with weight loss and softening. sprout-preventing chemicals, such as chlorpropham (cipc), can negatively impact the environment and human health. monthly thermal fogging with mint (mentha spicata l.) essential oil (meo) inhibited sprouting in eight potato cultivars during large-volume 6-month storage: the tubers remained firm with 38% lower weight loss after 140 days of storage. the sprout-inhibitory action may be ...201020390295
chemical composition and larvicidal evaluation of mentha, salvia, and melissa essential oils against the west nile virus mosquito culex pipiens.the volatile metabolites of wild-growing mentha spicata, m. longifolia, m. suaveolens, melissa officinalis, salvia fruticosa, s. pomifera subsp. calycina, and s. pomifera subsp. pomifera from greece were determined by gas chromatography and gas chromatography-mass spectrometry. the insecticidal properties of the analyzed essential oils were screened on culex pipiens larvae. additionally two of the main components of the essential oils, piperitenone oxide and 1,8-cineole were assayed against c. p ...201020405142
protection against lps-induced cartilage inflammation and degradation provided by a biological extract of mentha spicata.a variety of mint [mentha spicata] has been bred which over-expresses rosmarinic acid (ra) by approximately 20-fold. ra has demonstrated significant anti-inflammatory activity in vitro and in small rodents; thus it was hypothesized that this plant would demonstrate significant anti-inflammatory activity in vitro. the objectives of this study were: a) to develop an in vitro extraction procedure which mimics digestion and hepatic metabolism, b) to compare anti-inflammatory properties of high-rosma ...201020459798
levels of some microelements and essential heavy metals in herbal teas in serbia.levels of fe, mn, cu, zn, mo, co, ni, se, sn and al were determined in 14 medicinal plants from serbia, which are widely used in phytopharmacy as herbal teas. the following plants were investigated: yarrow (achillea millefolium l.), basil (ocimum hasilicum l.), st. john's wort (hypericum perforatum l.), peppermint (mentha x piperita l.), field horsetail (equisetum arvense l.), stinging nettle (urtica dioica l.), thyme (thymus serpyllum l.), maize silk (zea mays l. - maydis stigma), hibiscus (hib ...201425265817
the association between chromaticity, phenolics, carotenoids, and in vitro antioxidant activity of frozen fruit pulp in brazil: an application of chemometrics.a total of 19 brazilian frozen pulps from the following fruits: açai (euterpe oleracea), blackberry (rubus sp.), cajá (spondias mombin), cashew (anacardium occidentale), cocoa (theobroma cacao), coconut (cocos nucifera), grape (vitis sp.), graviola (annona muricata), guava (psidium guajava), papaya (carica papaya), peach (prunus persica), pineapple (ananas comosus), pineapple and mint (a. comosus and mentha spicata), red fruits (rubus sp. and fragaria sp.), seriguela (spondias purpurea), strawbe ...201424547813
a comparative analysis of the chemical composition, anti-inflammatory, and antinociceptive effects of the essential oils from three species of mentha cultivated in romania.this work was aimed at correlating the chemotype of three mentha species cultivated in romania with an in vivo study of the anti-inflammatory and antinociceptive effects of essential oils. the selected species were mentha piperita l. var. pallescens (white peppermint), mentha spicata l. subsp. crispata (spearmint), and mentha suaveolens ehrh. (pineapple mint). qualitative and quantitative analysis of the essential oils isolated from the selected mentha species was performed by gas chromatography ...201728208614
piperitenone oxide as toxic, repellent, and reproduction retardant toward malarial vector anopheles stephensi (diptera: anophelinae).anopheles stephensi (liston) is a well-known vector of malarial parasite in tropical countries. the developing trend of resistance in mosquitoes toward synthetic mosquitocidal agents makes their management extremely difficult. effectiveness of essential oils with aroma therapeutic values seems to be an emerging tool to combat this vector. piperitenone oxide isolated from essential oil of a new genotype, mentha spicata l. variety viridis, has been evaluated for larvicidal, ovicidal, oviposition-d ...200415311462
fumigant toxicity of plant essential oils against camptomyia corticalis (diptera: cecidomyiidae).the toxicity of 98 plant essential oils against third instars of cecidomyiid gall midge camptomyia corticalis (loew) (diptera: cecidomyiidae) was examined using a vapor-phase mortality bioassay. results were compared with that of a conventional insecticide dichlorvos. based on 24-h lc50 values, all essential oils were less toxic than dichlorvos (lc50, 0.027 mg/cm3). the lc50 of caraway (carum carvi l.) seed, armoise (artemisia vulgaris l.), clary sage (salvia sclarea l.), oregano (origanum vulga ...201222928313
adverse effects of herbal medicines: an overview of systematic reviews.this overview of systematic reviews (srs) aims to evaluate critically the evidence regarding the adverse effects of herbal medicines (hms). five electronic databases were searched to identify all relevant srs, with 50 srs of 50 different hms meeting our inclusion criteria. most had only minor weaknesses in methods. serious adverse effects were noted only for four hms: herbae pulvis standardisatus, larrea tridentate, piper methysticum and cassia senna. the most severe adverse effects were liver o ...201323472485
2,2-diphenyl-1-picrylhydrazyl as a screening tool for recombinant monoterpene biosynthesis.monoterpenes are a class of natural c10 compounds with a range of potential applications including use as fuel additives, fragrances, and chemical feedstocks. biosynthesis of monoterpenes in heterologous systems is yet to reach commercially-viable levels, and therefore is the subject of strain engineering and fermentation optimization studies. detection of monoterpenes typically relies on gas chromatography/mass spectrometry; this represents a significant analytical bottleneck which limits the p ...201323968454
medicinal and aromatic plant materials as nitrification inhibitors for augmenting yield and nitrogen uptake of japanese mint (mentha arvensis l. var. piperascens).pot experiments were conducted to evaluate the relative performance of medicinal and aromatic plant materials and dicyandiamide (dcd) as nitrification inhibitors to regulate transformation of n from urea. their effect on the efficiencies of use of n by japanese mint (mentha arvensis cv. hy 77) was tested. urea was coated with these materials viz., mentha spicata, artemisia annua or dcd at the rate of 5% (w/w) of fertilizer urea using an appropriate coating technique. nimin (tetranortriterpenoids ...200312688470
rhynchophorus ferrugineus midgut cell line to evaluate insecticidal potency of different plant essential oils.cell cultures can be a potent and strong tool to evaluate the insecticidal efficiency of natural products. plant essential oils have long been used as the fragrance or curative products around the world which means that they are safer to be used in close proximity of humans and mammals. in this study, a midgut cell line, developed from rhynchophorus ferrugineus (rpw-1), was used for screening essential oils from nine different plants. assays revealed that higher cell mortality was observed at 50 ...201525381034
comparative evaluation of five plant extracts and juices for nanoiron synthesis and application for hexavalent chromium reduction.the effectiveness of five plant extracts and juices, i.e. extracts of camellia sinensis (green tea, gt), syzygium aromaticum (clove, cl), mentha spicata (spearmint, sm), punica granatum juice (pomegranate, pg) and red wine (rw), for the production of nanoiron suspensions and their application for cr(vi) reduction was investigated. polyphenols contained in extracts act as reducing agents for iron ions in aqueous solutions, forming thus iron nanoparticles, and stabilize the nanoparticles produced ...201626356183
assessment of polyphenol coated nano zero valent iron for hexavalent chromium removal from contaminated waters.alternative plant extracts were examined as raw materials for the synthesis of nzvi from ferric solutions. four plants were selected for evaluation, i.e. camellia sinensis (green tea, gt), syzygium aromaticum (clove, cl), mentha spicata (spearmint, sm) and punica granatum (pomegranate, pg). based on the results obtained, it was concluded that the reduction of fe(iii) with the herb extracts is not complete. using the gt extract, approximately 28 mm of the initial 66 mm of fe (42.4 %) are reduced ...201525512186
functional and structural characterization of a (+)-limonene synthase from citrus sinensis.terpenes make up the largest and most diverse class of natural compounds and have important commercial and medical applications. limonene is a cyclic monoterpene (c10) present in nature as two enantiomers, (+) and (-), which are produced by different enzymes. the mechanism of production of the (-)-enantiomer has been studied in great detail, but to understand how enantiomeric selectivity is achieved in this class of enzymes, it is important to develop a thorough biochemical description of enzyme ...201728272875
antioxidant activity and cholinesterase inhibition studies of four flavouring herbs from alentejo.essential oils (eos) and aqueous extracts of aerial parts of four aromatic species, calamintha nepeta, foeniculum vulgare, mentha spicata and thymus mastichina, from southwest of portugal were characterised chemically and analysed in order to evaluate their antioxidant potential and cholinesterase inhibitory activities. the main components of eos were oxygenated monoterpenes, and aqueous extracts were rich in phenol and flavonoid compounds. eos and aqueous extracts presented a high antioxidant p ...201728076987
infusions and decoctions of mixed herbs used in folk medicine: synergism in antioxidant potential.infusions (herbal teas) and decoctions are used frequently to administer oral doses of herbs. although some herbs are used as single ingredients, they are often prepared as mixtures, as reported by numerous ethnobotanical surveys. the present work was carried out to identify the different types of interaction (synergistic, additive and antagonistic effects) which may be found in the antioxidant activity of preparations from mixtures of the popular herbs aloysia citrodora (lemon verbena), foenicu ...201121308820
effects of oral dosage form and storage period on the antioxidant properties of four species used in traditional herbal medicine.herbal infusions and decoctions in water are some of the most commonly consumed beverages in the world. although water is not a good solvent for many of the active components in herbs, liquid preparations are rich in several bioactive compounds. most of them have powerful antioxidant activity and have been related to medicinal herbs' properties. herein, decoctions and infusions in water of lemon-verbena (aloysia citrodora) aerial parts and leaves, fennel (foeniculum vulgare), pennyroyal (mentha ...201120740475
the effect of inhalation of citrus sinensis flowers and mentha spicata leave essential oils on lung function and exercise performance: a quasi-experimental uncontrolled before-and-after study.recently, there has been an increased interest in the effects of essential oils on athletic performances and other physiological effects. this study aimed to assess the effects of citrus sinensis flower and mentha spicata leaves essential oils inhalation in two different groups of athlete male students on their exercise performance and lung function.201727688737
extraction of antioxidants from plants using ultrasonic methods and their antioxidant capacity.the analytical method based on the hplc coupled with uv detection (hplc-uv) for the determination of selected antioxidants (i.e. esculetin, scopoletin, 7-hydroxycoumarine, rutin, xanthotoxin, 5-methoxypsoralen and quercetin) in plant material was developed. two ultrasonic extraction methods for the isolation of these compounds from the plants such as mentha longifolia l., mentha spicata l., ruta graveolens l., achyllea millefolium l., plantago lanceolata l. and coriandrum sativum l. were used. b ...200919072901
in vitro activity of twenty commercially available, plant-derived essential oils against selected dermatophyte species.the in vitro activity of twenty chemically defined essential oils (eos) obtained from boswellia sacra, citrus bergamia, c. limon, c. medica, cinnamomum zeylanicum, eucalyptus globulus, foeniculum vulgare, helichrysum italicum, illicium verum, litsea cubeba, mentha spicata, myrtus communis, ocimum basilicum, origanum majorana, o. vulgare, pelargonium graveolens, rosmarinus officinalis, santalum album, satureja montana, and thymus serpyllum was assayed against clinical animal isolates of microspor ...201526434145
fumigant toxicity of essential oils from basil and spearmint against two major pyralid pests of stored products.the fumigant activity of essential oil vapors distilled from sweet basil ocimum basilicum l. and spearmint mentha spicata l. (lamiaceae) were tested against two major stored products pests ephestia kuehniella (zeller) and plodia interpunctella (hübner) (lepidoptera: pyralidae). various oil doses (0.5, 2.5, 5, 50, 250, 500, 1,000, and 1,500 µl/liter air), for an exposure period of 24 h, were tested. the essential oils were subjected to gas chromatography-mass spectrometry analysis and revealed th ...201526470193
the efficacy of an herbal medicine, carmint, on the relief of abdominal pain and bloating in patients with irritable bowel syndrome: a pilot study.carmint contains total extracts of melissa officinalis, mentha spicata, and coriandrum sativum, which have antispasmodic, carminative, and sedative effects. as abdominal pain/discomfort and bloating are commonly observed in patients with irritable bowel syndrome, we decided to evaluate the effectiveness of carmint in relieving these symptoms in irritable bowel syndrome patients. we randomly assigned 32 irritable bowel syndrome patients to receive either carmint or placebo, plus loperamide or psy ...200616868824
mentha x piperita, mentha spicata and effects of their essential oils on meloidogyne in soil.six peppermint (mentha x piperita) and six spearmint (m. spicata) pi accessions were inoculated with meloidogyne incognita race 3 and m. arenaria race 2, under greenhouse conditions. no galls formed on roots of any of the plants inoculated with 1,800 eggs/pot. fewer than two galls per root system formed on three pi accessions of peppermint inoculated with m. incognita at 5,400 eggs/pot. only one peppermint accession developed galls when inoculated with m. arenaria, whereas none of the spearmint ...199619277186
nematicidal activity of essential oils and their components against the root-knot nematode.abstract nematicidal activity of essential oils extracted from 27 spices and aromatic plants were evaluated in vitro and in pot experiments. twelve of the twenty-seven essential oils immobilized more than 80% of juveniles of the root-knot nematode meloidogyne javanica at a concentration of 1,000 mul/liter. at this concentration, most of these oils also inhibited nematode hatching. essential oils of carum carvi, foeniculum vulgare, mentha rotundifolia, and mentha spicata showed the highest nemati ...200018944489
biocidal compounds from mentha sp. essential oils and their structure-activity relationships.essential oils from greek mentha species showed different chemical compositions for two populations of m. pulegium, characterized by piperitone and pulegone. mentha spicata essential oil was characterized by endocyclic piperitenone epoxide, piperitone epoxide, and carvone. the bioactivities of these essential oils and their components have been tested against insect pests (leptinotarsa decemlineata, spodoptera littoralis and myzus persicae), root-knot nematodes (meloydogine javanica) and plants ...201727770481
expression, crystallization and structure elucidation of γ-terpinene synthase from thymus vulgaris.the biosynthesis of γ-terpinene, a precursor of the phenolic isomers thymol and carvacrol found in the essential oil from thymus sp., is attributed to the activitiy of γ-terpinene synthase (tps). purified γ-terpinene synthase from t. vulgaris (tvtps), the thymus species that is the most widely spread and of the greatest economical importance, is able to catalyze the enzymatic conversion of geranyl diphosphate (gpp) to γ-terpinene. the crystal structure of recombinantly expressed and purified tvt ...201626750479
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