| development of a gene reporter system in moderately halophilic bacteria by employing the ice nucleation gene of pseudomonas syringae. | the expression of the ice nucleation gene inaz of pseudomonas syringae in several moderate halophiles was investigated to establish its utility as a reporter for promoter activity and gene expression studies in these biotechnologically and environmentally important bacteria. a promoterless version of inaz was introduced in two different restriction sites and at both orientations in a recombinant plasmid able to replicate in moderate halophiles and, in particular, within the sequence of its phe1 ... | 1995 | 8526492 |
| production and biochemical characterization of an alpha-amylase from the moderate halophile halomonas meridiana. | extracellular amylase production by the moderate halophile halomonas meridiana was optimized and the enzyme was characterized biochemically. the highest amylase production was achieved by growing h. meridiana cultures in media with 5% salts and starch, in the absence of glucose until the end of the exponential phase. the amylase exhibited maximal activity at ph 7.0, being relatively stable in alkaline conditions. optimal temperature and salinity for activity were 37 degrees c and 10% nacl, respe ... | 2000 | 10650204 |
| the alpha-amylase gene amyh of the moderate halophile halomonas meridiana: cloning and molecular characterization. | two types of tn1732-induced mutants defective in extracellular amylase activity were isolated from the moderate halophile halomonas meridiana dsm 5425. type i mutants displayed amylase activity in the periplasm, and were unable to use any of the carbon sources tested, including starch and its hydrolysis product maltose. the type ii mutant was affected in the gene responsible for the synthesis of the extracellular alpha-amylase. this gene (amyh) was isolated by functional complementation of mutan ... | 2000 | 10784044 |
| halomonas neptunia sp. nov., halomonas sulfidaeris sp. nov., halomonas axialensis sp. nov. and halomonas hydrothermalis sp. nov.: halophilic bacteria isolated from deep-sea hydrothermal-vent environments. | to assess the physiological and phylogenetic diversity of culturable halophilic bacteria in deep-sea hydrothermal-vent environments, six isolates obtained from low-temperature hydrothermal fluids, sulfide rock and hydrothermal plumes in north and south pacific ocean vent fields located at 1530-2580 m depth were fully characterized. three strains were isolated on media that contained oligotrophic concentrations of organic carbon (0.002 % yeast extract). sequencing of the 16s rrna gene indicated t ... | 2004 | 15023967 |
| halomonas boliviensis sp. nov., an alkalitolerant, moderate halophile isolated from soil around a bolivian hypersaline lake. | halomonas boliviensis sp. nov. is proposed for two moderately halophilic, psychrophilic, alkalitolerant bacteria, lc1(t) (=dsm 15516(t)=atcc baa-759(t)) and lc2 (=dsm 15517=atcc baa-760), both of which were isolated from a soil sample around the lake laguna colorada, located at 4300 m above sea level in the south-west region of bolivia. the bacteria are aerobic, motile, gram-negative rods that produce colonies with a cream pigment. moreover, they are heterotrophs that are able to utilize various ... | 2004 | 15143014 |
| [enrichment and diversity analyis of arsenite-resistant bacteria in deep sea sediment samples from southwest indian ocean ridge]. | the aim of this study was to assess the diversity of arsenite-resistant bacteria in deep sea sediment of the southwest indian ocean ridge. | 2008 | 19160816 |
| utilization of mucus from the coral acropora palmata by the pathogen serratia marcescens and by environmental and coral commensal bacteria. | in recent years, diseases of corals caused by opportunistic pathogens have become widespread. how opportunistic pathogens establish on coral surfaces, interact with native microbiota, and cause disease is not yet clear. this study compared the utilization of coral mucus by coral-associated commensal bacteria ("photobacterium mandapamensis" and halomonas meridiana) and by opportunistic serratia marcescens pathogens. s. marcescens pdl100 (a pathogen associated with white pox disease of acroporid c ... | 2009 | 19395569 |
| halomonas andesensis sp. nov., a moderate halophile isolated from the saline lake laguna colorada in bolivia. | a moderately halophilic, motile, gram-negative, rod-shaped bacterium, strain lc6(t), was isolated from a water sample of lake laguna colorada in the bolivian andes. the major cellular fatty acids were c(18 : 1)omega7c, iso-c(16 : 1)omega7c 2-oh, c(16 : 0) and c(12 : 0) 3-oh. the respiratory ubiquinones found in strain lc6(t) were q-9 (97 %) and q-8 (3 %). strain lc6(t) was aerobic, heterotrophic, and able to utilize various carbohydrates and other substrates as carbon source. the g+c content of ... | 2010 | 19656933 |
| halomonas titanicae sp. nov., a halophilic bacterium isolated from the rms titanic. | a gram-negative, heterotrophic, aerobic, non-endospore-forming, peritrichously flagellated and motile bacterial strain, designated bh1(t), was isolated from samples of rusticles, which are formed in part by a consortium of micro-organisms, collected from the rms titanic wreck site. the strain grew optimally at 30-37°c, ph 7.0-7.5 and in the presence of 2-8 % (w/v) nacl. we carried out a polyphasic taxonomic study in order to characterize the strain in detail. phylogenetic analyses based on 16s r ... | 2010 | 20061494 |
| catabolite regulation of enzymatic activities in a white pox pathogen and commensal bacteria during growth on mucus polymers from the coral acropora palmata. | colonization of host mucus surfaces is one of the first steps in the establishment of coral-associated microbial communities. coral mucus contains a sulfated glycoprotein (in which oligosaccharide decorations are connected to the polypeptide backbone by a mannose residue) and molecules that result from its degradation. mucus is utilized as a growth substrate by commensal and pathogenic organisms. two representative coral commensals, photobacterium mandapamensis and halomonas meridiana, differed ... | 2009 | 20095241 |
| identification of new members within suites of amphiphilic marine siderophores. | marine bacterial isolates vibrio sp. hc0601c5 and halomonas meridiana str. hc4321c1 were isolated off the coast of southern california and were found to produce an expanded suite of previously identified amphiphilic siderophores. specifically two new members of the amphibactin family, amphibactins s and t, which have a c14:1 ω-7 fatty acid and a saturated c12 fatty acid, respectively, were produced by vibrio sp. hc0601c5. these siderophores are produced in addition to a number of previously desc ... | 2011 | 20853137 |
| biofilm formation in moderately halophilic bacteria is influenced by varying salinity levels. | bacteria in a biofilm have a co-dependent lifestyle resulting in a harmonized and complex coordination of the bacterial cells within an exopolysaccharide (eps) matrix. we hypothesized that biofilm formation and eps production in salt-tolerant bacteria are helpful for plant growth improvement in saline soil, but that they are influenced differently. to investigate this hypothesis, we tested the effect of different salinity levels on the biofilm formation of the bacterial strains paa6 (halomonas m ... | 2011 | 22144335 |
| integrated molecular, physiological and in silico characterization of two halomonas isolates from industrial brine. | two haloalkaliphilic bacteria isolated from industrial brine solutions were characterized via molecular, physiological, and in silico metabolic pathway analyses. genomes from the organisms, designated halomonas bc1 and bc2, were sequenced; 16s ribosomal subunit-based phylogenetic analysis revealed a high level of similarity to each other and to halomonas meridiana. both strains were moderate halophiles with near optimal specific growth rates (≥60 % μ max) observed over <0.1-5 % (w/v) nacl and ph ... | 2016 | 26888357 |
| draft genome sequence of halomonas meridiana r1t3 isolated from the surface microbiota of the caribbean elkhorn coral acropora palmata. | members of the gammaproteobacterial genus halomonas are common in marine environments. halomonas and other members of the oceanospirillales have recently been identified as prominent members of the surface microbiota of reef-building corals. halomonas meridiana strain r1t3 was isolated from the surface mucus layer of the scleractinian coral acropora palmata in 2005 from the florida keys. this strain was chosen for genome sequencing to provide insight into the role of commensal heterotrophic bact ... | 2015 | 26451236 |
| physiological features of halomonas lionensis sp. nov., a novel bacterium isolated from a mediterranean sea sediment. | a novel halophilic bacterium, strain rhs90(t), was isolated from marine sediments from the gulf of lions, in the mediterranean sea. its metabolic and physiological characteristics were examined under various cultural conditions, including exposure to stressful ones (oligotrophy, high pressure and high concentrations of metals). based on phylogenetic analysis of the 16s rrna gene, the strain was found to belong to the genus halomonas in the class gammaproteobacteria. its closest relatives are hal ... | 2014 | 25086262 |
| halomonas songnenensis sp. nov., a moderately halophilic bacterium isolated from saline and alkaline soils. | a moderately halophilic bacterium (strain neau-st10-39t) was isolated from saline and alkaline soils in the oilfield of daqing city, heilongjiang province, china. the strain was strictly aerobic, gram-stain-negative, rod-shaped and motile by peritrichous flagella. its colonies were yellow. it grew at nacl concentrations of 0.2-15% (w/v) (optimum 4%, w/v), at temperatures of 4-40 °c (optimum 35 °c) and at ph 5-10 (optimum ph 7). it did not produce acids from sugars or alcohols. its dna g+c conten ... | 2014 | 24510978 |
| induction of apoptosis in cancer cell lines by the red sea brine pool bacterial extracts. | marine microorganisms are considered to be an important source of bioactive molecules against various diseases and have great potential to increase the number of lead molecules in clinical trials. progress in novel microbial culturing techniques as well as greater accessibility to unique oceanic habitats has placed the marine environment as a new frontier in the field of natural product drug discovery. | 2013 | 24305113 |