| [involvement of mycoplasma ovipneumoniae in enzootic proliferating interstitial pneumonia in sheep]. | | 1979 | 113871 |
| the experimental infection of specific pathogen free lambs with mycoplasma ovipneumoniae. | six colostrum-deprived spf lambs inoculated endobronchially with a second passage broth culture of a scottish strain of mycoplasma ovipneumoniae, were killed in batches of two at seven, 14 and 28 days post-inoculation. one lamb from each batch showed macroscopic and microscopic lung lesions similar to but milder than those described for respiratory mycoplasmoses in other species of animals and exhibited minor clinical symptoms. mycoplasma were recovered from all infected but from no control anim ... | 1976 | 133436 |
| ovine pulmonary cytomegalovirus. | cytomegalic cells with intranuclear inclusions were identified in lung tissue from one of two weanling western lambs with pneumonia and mycoplasma ovipneumoniae infection. on the basis of light and electron microscopy, cytomegalovirus infection was diagnosed. arrays of viral particles whose morphology was consistent with that of viruses of the herpes group were in the intranuclear inclusions of cytomegalic cells. | 1979 | 222035 |
| an in vitro study of the replication of mycoplasma ovipneumoniae. | | 1979 | 292899 |
| experimental studies of chronic pneumonia of sheep. | strains of mycoplasma ovipneumoniae and pasteurella haemolytica isolated from sheep affected with chronic pneumonia were inoculated by endobronchial route to conventionally-reared and spf (specific pathogen-free) lambs. changes resembling those of the naturally-occurring disease were produced in most lambs given the organisms in combination and in some given m. ovipneumoniae alone. similar but less extensive changes were seen in spf lambs and fewer animals were affected. different strains of m. ... | 1979 | 318238 |
| isolation of mycoplasma ovipneumoniae from spanish and angora goats. | | 1979 | 475095 |
| [incidence of mycoplasma ovipneumoniae in sheep]. | out a total number of 19 mycoplasma strain from diseased sheeps, 5 strains were used for further investigations. the 5 strains were differentiated as mycoplasma ovipneumoniae. the relation between mycoplasma findings and the disease is discussed. | 1977 | 901125 |
| atypical mycoplasmas from sheep in great britain and australia identified as mycoplasma ovipneumoniae. | representative strains of "lacy-colony" mycoplasmas isolated from sheep in great britain and victoria (australia) were classified as m ovipneumoniae following comparison with strain y98 of this species. the taxonomic description of m ovipneumoniae is extended and y98 is proposed as the type strain. a brief description is given of the isolation of m ovipneumoniae from sheep in east anglia. | 1976 | 936439 |
| mycoplasma ovipneumoniae demonstrated in icelandic sheep. | | 1976 | 952254 |
| mycoplasmas and ovine keratoconjunctivitis. | the clinical course of an outbreak of keratoconjunctivitis in housed lambs and their dams was followed. signs were transient generally and became severe in only a small proportion of lambs. the outbreak became most obvious when the lambs were 46 to 55 days old, when 46.9 per cent were affected. mycoplasma conjunctivae isolations, confirmed by comparison with the type strain by biochemical and serological reactions, increased to 62.1 per cent of all eyes swabbed, but no correlation could be demon ... | 1976 | 969200 |
| the prevalence of mycoplasma ovipneumoniae and mycoplasma arginini in the respiratory tract of sheep. | | 1975 | 1058377 |
| in vitro sensitivity of new zealand strains of mycoplasma ovipneumoniae to some antibiotics. | | 1975 | 1060971 |
| isolation of mycoplasma ovipneumoniae from sheep with chronic pneumonia. | | 1975 | 1162872 |
| isolation of mycoplasma ovipneumoniae from sheep with pneumonia. | | 1975 | 1231559 |
| the comparison and characterisation of glycolytic mycoplasmas isolated from the respiratory tract of sheep. | nine strains of glycolytic mycoplasmas isolated from the respiratory tract of apparently healthy sheep, pneumonic sheep and sheep with pulmonary adenomatosis (spa) were compared with a queensland strain (y98) of mycoplasma ovipneumoniae. all strains were very similar in their reactions in 14 biochemical tests and in their sensitivities to optochin, digitonin, sodium polyanethol sulphonate, and 11 antibiotics. polyacrylamide-gel electrophoresis and serological cross-reactions by the agar-gel doub ... | 1976 | 1263247 |
| mycoplasma ovipneumoniae infection in zimbabwean goats and sheep. | the prevalence of mycoplasma ovipneumoniae (mo) in three communal lands flocks of goats on the outskirts of harare is described. in a single sampling of nasal swabs from 172 randomly selected, apparently healthy goats, 112 animals were identified as carriers of mo. the three flocks had a history of occasional deaths, following chronic and subacute pneumonia associated with isolation of mo as the only identifiable pathogen. attempts to induce clinical pneumonia in groups of 6 to 8-week-old kids a ... | 1992 | 1430348 |
| a study of the heterogeneity of isolates of mycoplasma ovipneumoniae from sheep in new zealand. | to investigate the heterogeneity of mycoplasma ovipneumoniae, sixty isolates from three sheep on each of twenty farms were examined by restriction endonuclease analysis (rea) and sds-page. all were found to be different except for three isolates obtained from one farm. the protein and rea patterns of individual isolates were both highly reproducible and remained unchanged following long term passage (approximately 400 generations) in vitro. no plasmids were detected in the twelve strains which w ... | 1991 | 1771756 |
| the isolation of multiple strains of mycoplasma ovipneumoniae from individual pneumonic sheep lungs. | the heterogeneity of mycoplasma ovipneumoniae isolates from the lungs of sheep with chronic non-progressive pneumonia (cnp) from the same flock raised the possibility that multiple isolates derived from one lung were not all identical. to test this hypothesis, thirty isolates were obtained from each of six pneumonic sheep lungs at slaughter. four lungs had relatively severe lesions and from each of these, three or four strains of m. ovipneumonia, distinguishable by rea and in most cases by sds-p ... | 1991 | 1771757 |
| cross-reacting antigens between mycoplasma ovipneumoniae and other species of mycoplasma of animal origin, shown by elisa and immunoblotting with reference antisera. | using enzyme-linked immunosorbent assays with mycoplasma ovipneumoniae as antigen, the cross-reactivity of antigens between this species and 22 other mycoplasma species was examined using reference polyclonal antisera. significant cross-reactivity with m. ovipneumoniae was demonstrated by five species, only, viz. m. bovoculi, m. dispar, m. flocculare, m. hyopneumoniae and m. hyorhinis. using one-dimensional sds-page and immunoblotting techniques with homologous and heterologous antisera, cross-r ... | 1991 | 2024444 |
| the humoral immune response of lambs experimentally infected with mycoplasma ovipneumoniae. | using sera from lambs experimentally infected with mycoplasma ovipneumoniae and pasteurella haemolytica, the development of a good humoral immune response to m. ovipneumoniae was detected by elisa. the antibody titres peaked 41 days post-infection and good antibody titres were maintained over the 16-week experimental period. immunoblotting revealed that antibodies to specific antigens appeared in the sera in a sequential manner, some being seen shortly after infection and others developing only ... | 1990 | 2238460 |
| polypeptide and antigenic variability among strains of mycoplasma ovipneumoniae demonstrated by sds-page and immunoblotting. | comparison of the polypeptide patterns of 22 isolates of m. ovipneumoniae by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (sds-page) showed a marked degree of heterogeneity with only limited groupings identifiable. of the 50 major polypeptides identified in one strain (956/2), 35 were shown to be antigenic using immunoblotting with a homologous polyclonal serum. radioimmune precipitation of 125i-surface-labelled proteins and phase partition using triton x-114 detergent indicated th ... | 1990 | 2305546 |
| [natural and experimental infections of lambs with mycoplasma ovipneumoniae]. | mycoplasma (m.) ovipneumoniae was isolated pure or mixed with bacteria from 47 lungs of lambs of 14 in 22 tested flocks. m. ovipneumoniae was obtained as pure culture in cases of mild bronchopneumonia. experimental intratracheal or intranasal infection caused several days of rising body temperature above 39.7 degrees c. nasal discharge, coughing, and dyspnea did not occur. m. ovipneumoniae was successfully re-isolated from nasal swabs, beginning 2 d from infection. lobular catarrhal bronchopneum ... | 1989 | 2533484 |
| serologic survey for mycoplasma ovipneumoniae in free-ranging dall sheep (ovis dalli) in alaska. | indirect hemagglutination tests on sera from 251 dall sheep (ovis dalli) from interior alaska collected during the period 1979 to 1987 revealed no evidence of exposure to mycoplasma ovipneumoniae. apparently, this potentially fatal disease agent has not been introduced into free-ranging dall sheep populations. in the interest of continued health of such dall sheep, strict enforcement of domestic animal health regulations and prudent land use practices are clearly indicated. | 1989 | 2810563 |
| [nasal and pulmonary flora in the goat]. | on the basis of bacteriological examinations carried out on 75 intranasal swabs and 80 goat lung punctures, aerobic, aero-anaerobic respiratory microbes and mycoplasma were studied. mycoplasma ovipneumoniae was isolated both from nasal flora (37%) and from the lungs (27.5%) as well as pasteurella spp which accounted respectively for 24% and 12.5%. the nasal flora are characterised by the presence of a gram-negative strain included in the genus moraxella and connected with moraxella bovis species ... | 1989 | 2817731 |
| comparison of mycoplasma ovipneumoniae isolates using bacterial restriction endonuclease dna analysis and sds-page. | sixteen isolates of mycoplasma ovipneumoniae recovered from the nasal tract or lungs of sheep from different flocks in new zealand were examined by bacterial restriction endonuclease dna analysis (brenda) using ecor1 and by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (sds-page). all isolates gave brenda patterns which differed entirely from one another. following 20 serial passages (corresponding to approximately 67 generations) of an isolate, no change was detected in the brenda ... | 1985 | 3006324 |
| isolation and identification of mycoplasmas from the nasal cavity of sheep. | mycoplasmas isolated from the nasal cavity of sheep in a ram test station were examined to determine their identity and prevalence. specimens were obtained for mycoplasmal culture in 1980, 1982, and 1983 from 558 sheep, and mycoplasmas were isolated from 630 specimens from 320 sheep (57.3%). the isolates were characterized and differentiated into groups on the basis of sensitivity to digitonin, fermentation of glucose, and hydrolysis of arginine. isolates in some groups were further characterize ... | 1988 | 3056135 |
| an epizootic of mycoplasma ovipneumoniae infection in captive dall's sheep (ovis dalli dalli). | in late spring of 1986, 10 of 23 dall's sheep (ovis dalli dalli) at the metropolitan toronto zoo were moved to a new exhibit, where all developed severe respiratory signs refractory to anthelmintic and antibiotic therapy. in july, two animals died with chronic active bronch-pneumonia, and a third was euthanized because of pneumonia several months later. bacteria were not isolated from the lungs of the first, steptococci and pasteurella hemolytica were isolated from the other two, respectively; m ... | 1988 | 3193557 |
| microbiological and serological studies on caprine pneumonias in oman. | eight of 10 typical cases of contagious caprine pleuro-pneumonia in oman yielded strain f38-like mycoplasmas from the lungs in high titre, but no other mycoplasmas: both negative animals had been treated with tylosin shortly before death. among 21 other lungs examined three of six cases of acute pneumonia yielded mycoplasma ovipneumoniae; one also yielded m capricolum. m ovipneumoniae was also isolated from all eight cases of chronic pneumonia sampled from an abattoir, and from the lungs of thre ... | 1988 | 3287526 |
| infectious agents in respiratory disease of housed, fattening lambs in northern ireland. | a two-year longitudinal, microbiological and pathological survey of respiratory disease in lambs housed for fattening at three-and-a-half to four months of age was undertaken. in the first year samples of nasal mucus and blood were taken from lambs each week for the first nine weeks after entry to a fattening unit and each week one lamb was examined post mortem. in the second year two additional fattening units were included in the survey, when samples of blood and nasal mucus were taken from la ... | 1988 | 3381463 |
| [first isolation of mycoplasma ovipneumoniae in senegal]. | | 1987 | 3453499 |
| assessment of immunity to pasteurella haemolytica in sheep by in vitro methods. | the sera of conventionally-reared sheep which had been experimentally infected with pasteurella haemolytica serotype a2 and mycoplasma ovipneumoniae were compared with those of animals from the same source which had been vaccinated 3 times with an alhydrogel-adsorbed mixture of cell-wall extract and heat-killed cells of p. haemolytica a2 emulsified in freund's incomplete adjuvant. both groups of animals had high titres of anti-p. haemolytica a2 igg by elisa, high opsonic indices in a phagocytic ... | 1986 | 3539670 |
| comparison of single strains of four serotypes of pasteurella haemolytica biotype a in experimental pneumonia of sheep. | single strains of serotypes a1, a2, a7 and a9 of pasteurella haemolytica were separately used in combination with mycoplasma ovipneumoniae to reproduce pneumonia. macroscopically and microscopically the pneumonias associated with individual serotypes were similar and it is concluded that serotypes of p haemolytica isolated with low frequency in field disease may be equally virulent to common serotypes. | 1986 | 3704319 |
| experimental studies on the pathogenicity of mycoplasma ovipneumoniae and mycoplasma arginini for the respiratory tract of goats. | mycoplasma ovipneumoniae and mycoplasma arginini were the species of mollicutes most commonly isolated from 175 goats with respiratory disease in ontario. the pathogenicity of m. ovipneumoniae, strain b321b and m. arginini, strain d53e, was assessed in goats following endobronchial inoculation. one out of three two year old goats developed fever after inoculation with a pure culture of strain b321b, and it had extensive subacute fibrinous pleuritis when necropsied three weeks later. neither of t ... | 1986 | 3742358 |
| the pathogenicity of some ovine or caprine mycoplasmas in the lactating mammary gland of sheep and goats. | mycoplasma ovipneumoniae, m. arginini and m. conjunctivae were each inoculated into the lactating mammary gland of goats and sheep. m. ovipneumoniae was mastitogenic in both species, causing persistent colonization, raised lacteal cell numbers, decreased milk output and separation of milk into 3 layers throughout the periods of observation in virtually all animals. its effects in sheep were variable, however, and appeared to depend less on the strain and titre of inoculum than on individual vari ... | 1985 | 4031126 |
| the pathogenicity of mycoplasma ovipneumoniae and mycoplasma arginini in ovine and caprine tracheal organ cultures. | the effects of m. ovipneumoniae and m. arginini on tracheal organ cultures prepared from a neonatal kid and a foetal lamb were studied. both organisms were isolated from the cultures throughout the 14 days of observation. m. ovipneumoniae produced ciliostasis and loss of cilia, confirmed by scanning electron microscope (sem), after 4 days. these effects were sudden and profound in lamb explants, and gradual and less pronounced in kid explants. clusters of organisms attached to epithelial surface ... | 1985 | 4067018 |
| colonisation of the respiratory tract of lambs by strains of mycoplasma ovipneumoniae. | the age and time of year when colonisation of the nasal cavity of lambs by mycoplasma ovipneumoniae occurs; the persistence of the organism, and its prevalence in the lungs at slaughter were examined in 2 flocks of sheep in new zealand. no colonisation had occurred at the time of weaning at 6-7 weeks, but m. ovipneumoniae was recovered from most lambs on at least one occasion before they were slaughtered when about 8 months old. in most cases, colonisation of the nasal cavity by m. ovipneumoniae ... | 1985 | 4095899 |
| the effect of mycoplasma ovipneumoniae and pasteurella haemolytica on specific pathogen-free lambs. | | 1982 | 6211466 |
| the effects of different strains of mycoplasma ovipneumoniae on specific pathogen-free and conventionally-reared lambs. | | 1982 | 6211467 |
| keratoconjunctivitis infectiosa ovis (kio), 'pink eye' or 'zere oogjes' (a survey). | the occurrence of keratoconjunctivitis infectiosa ovis (kio) in the netherlands was reported by hofland et al. in 1969 for the first time. at present the disease is well known in the netherlands. nevertheless there are still questions about the causative agent and the most effective and easiest therapy. most authors suppose that the disease is caused by colesiota conjunctivae, although others mention infections by other chlamydia, mycoplasma conjunctivae, mycoplasma ovipneumoniae, acholeplasma o ... | 1983 | 6356577 |
| experimental infection of sheep with mycoplasma ovipneumoniae and pasteurella haemolytica. | a group of caesarian-derived, colostrum-deprived lambs was inoculated intranasally and intratracheally with a virulent mycoplasma ovipneumoniae isolate selected from ovine mammary studies and propagated in an ovine mammary gland. other groups of lambs were inoculated with m. ovipneumoniae in combination with pasteurella haemolytica type al or p. haemolytica alone. the m. ovipneumoniae isolate alone did not induce any specific pneumonic lesions in the lambs and when combined with p. haemolytica t ... | 1984 | 6506448 |
| comparison of virulence of ovine respiratory mycoplasmas in the mouse mammary gland. | the virulence of isolates of mycoplasma ovipneumoniae and m. arginini from pneumonic and unaffected ovine lungs was compared in a mouse mammary gland model. the isolates varied in their ability to induce a neutrophilic response in the mammary gland. a moderate to severe form of mastitis was induced by 3 m. ovipneumoniae isolates recovered from pneumonic lungs, while the remaining m. ovipneumoniae isolates from pneumonic lungs and those from unaffected lungs induced a very mild histopathological ... | 1984 | 6541843 |
| electron microscopic studies of the interaction between ovine alveolar macrophages and mycoplasma ovipneumoniae in vitro. | the interaction between mycoplasma ovipneumoniae and ovine alveolar macrophages with and without the addition of antibody was observed by both scanning and transmission electron microscopy. mycoplasma ovipneumoniae organisms had the ability to attach to the plasma membrane of macrophages without inducing phagocytosis although they stimulated mitotic division in early cultured cells. the addition of specific antibody to mycoplasma--macrophage cultures stimulated phagocytosis of the organisms. mac ... | 1983 | 6659313 |
| [respiratory diseases in sheep due to mycoplasma ovipneumoniae]. | mycoplasma ovipneumoniae was isolated from sheep and lambs affected with a respiratory disease. it was established that the pneumonic disease caused by this organism was a severe one, with high mortality rate in young lambs. adult sheep and older lambs remained chronically affected. susceptibility varied, depending on the breed of the animals, some imported animals running a more severe course of the disease than the local ones. the disease was artificially induced in lambs at the joint infectio ... | 1982 | 7157655 |
| studies of the pathology and microbiology of pneumonic lungs of lambs. | the characteristics of lung lesions of slaughtered lambs and the presence of microorganisms in the lesions were investigated. pneumonia was detected in 500 (3.6%) of 13,588 lambs. macroscopical lesions were of atypical pneumonia and were often seen in the pars cranialis of lobus cranialis dexter. histologically, proliferative pneumonia was detected in most cases and was often accompanied by exudative characteristics. pasteurella haemolytica was isolated from 258 (51.6%) and mycoplasma ovipneumon ... | 1994 | 7895625 |
| [etiologic and pathologic study of respiratory disease in lambs from intensive breeding facilities in southern spain]. | between 1991 and 1993, it was observed epidemiologically that respiratory disturbances in lambs are associated with high temperatures during the summer. the etiological agent isolated is principally mycoplasma ovipneumoniae; moreover, actinobacillus pleuropneumoniae biovar a has been isolated in a high number of samples. histopathologically, an interstitial bronchopneumonia was the main lesional finding; this lesion is associated with previous mycoplasma infection. | 1996 | 8767768 |
| respiratory infections of sheep. | sheep respiratory infections appear as differing clinical syndromes. mild, acute infections are usually due to parainfluenza 3 (pi3) virus. a mild but chronic respiratory problem in lambs under 1 year old is thought to be caused by mycoplasma ovipneumoniae probably in association with pasteurella and pi3. acute bacterial pneumonia usually results from infection with pasteurella of biotype a. infection with pi3 can initiate invasion by pasteurella. bordetella parapertussis infection has also been ... | 1996 | 8800542 |
| detection of mycoplasma ovipneumoniae and pasteurella haemolytica antigens by an immunoperoxidase technique in pneumonic ovine lungs. | four hundred twenty pneumonic lungs from lambs were examined for mycoplasma ovipneumoniae and pasteurella haemolytica by an immunoperoxidase technique using an extravidin-biotin-peroxidase complex method in formalin-fixed, paraffin-embedded sections. histologic examination of tissue sections revealed strong positive reactions in 60.9% and 68.3% of the lungs against m. ovipneumoniae and p. haemolytica, respectively. m. ovipneumoniae and p. haemolytica antigens were observed at the surface and/or ... | 1996 | 8826008 |
| phylogeny of some mycoplasmas from ruminants based on 16s rrna sequences and definition of a new cluster within the hominis group. | almost complete (> 96%) 16s rrna sequences from nine ruminant mycoplasmas have been determined by solid-phase dna sequencing. polymorphisms were found in four of the 16s rrna sequences, which indicated the existence of two different rrna operons. seven polymorphisms were found in mycoplasma agalatiae, three were found in mycoplasma bovis, one was found in mycoplasma alkalescens, and one was found in mycoplasma bovirhinis. the sequence data were used for construction of phylogenetic trees. all bu ... | 1996 | 8863441 |
| expression of functions by normal sheep alveolar macrophages and their alteration by interaction with mycoplasma ovipneumoniae. | normal sheep alveolar macrophages collected by bronchial lavage were exposed to live or heat-killed mycoplasma ovipneumoniae organisms, and their capability to ingest staphylococcus aureus and to elicit antibody-dependent cellular cytotoxicity against sensitized chicken red blood cells was tested. controls consisted of non-infected macrophages in m199 medium. in addition, the effect of m. ovipneumoniae on expression of surface molecules on these sheep alveolar macrophages was determined. the per ... | 1997 | 9451459 |
| field isolates of mycoplasma ovipneumoniae exhibit distinct cytopathic effects in ovine tracheal organ cultures. | ovine tracheal ring explants were infected with four different mycoplasma ovipneumoniae and one m. arginini field isolate and their ability to induce cytopathic effects was tested by measuring ciliary activity and intracellular calmodulin release. infected tracheal rings showed significantly decreased ciliary activity as compared to the non-infected control rings. there were, however, marked differences between isolates in the onset and severity of the effects which correlated with their ability ... | 1998 | 9557125 |
| demonstration of a capsule on mycoplasma ovipneumoniae. | to examine mycoplasma ovipneumoniae for presence of a capsule and its potential role in adherence. | 1998 | 9582956 |
| pasteurella haemolytica complicated respiratory infections in sheep and goats. | respiratory infections which commonly occur in sheep and goats often result from adverse physical and physiological stress combined with viral and bacterial infections. inevitably, pasteurella haemolytica pneumonia occurs as a result of these interactions. in this review, we present recent advances in research on the complex etiology of pneumonia involving p. haemolytica. initially stress, induced by factors such as heat, overcrowding, exposure to inclement weather, poor ventilation, handling an ... | 1998 | 9689740 |
| occurrence of autoantibodies to cilia in lambs with a 'coughing syndrome'. | a respiratory disease of lambs that has been termed the 'coughing syndrome' has been observed in the mid-western region of the united states of america. mycoplasma ovipneumoniae (m. ovipneumoniae) and mycoplasma arginini (m. arginini) were routinely isolated from the respiratory tract of lambs with this disease. a high level of antibodies reactive with ovine cilia of the upper respiratory tract was detected in the sera from many of the lambs in affected flocks but not in sera of lambs from unaff ... | 1998 | 9730216 |
| differential serologic response to mycoplasma ovipneumoniae and mycoplasma arginini in lambs affected with chronic respiratory disease. | an enzyme-linked immunoabsorbent assay (elisa) was used to evaluate the levels of antibodies to mycoplasma ovipneumoniae and m. arginini in lambs with chronic respiratory disease. sera were obtained from lambs in several flocks at various stages of the clinical disease and tested with sodium dodecyl sulfate (sds)-treated m. ovipneumoniae and m. arginini whole cells and a crude capsular extract of m. ovipneumoniae as the antigens. there were low levels of antibody to m. ovipneumoniae in flocks sa ... | 1999 | 9925209 |
| mycoplasmas isolated from the respiratory tract of cattle and goats in tanzania. | a microbiological study of the mycoplasma flora in the respiratory tracts of cattle and goats in selected regions of tanzania is described. in the examination of cattle, mycoplasmas were isolated from 60 (17.8%) of the 338 examined lung samples, 8 (47.1%) of the 17 lymph nodes, 4 (13.3%) of the 30 pleural fluid samples and 4 (3.9%) of the 103 nasal swabs examined. all the isolates were identified as mycoplasma mycoides subsp. mycoides, small colony type except for one isolate from pleural fluid ... | 2000 | 11126579 |
| demonstration of mycoplasma capricolum subsp. capripneumoniae and mycoplasma mycoides subsp. mycoides, small colony type in outbreaks of caprine pleuropneumonia in eastern tanzania. | an outbreak of caprine pleuropneumonia involving about 1200 goats in the coast and morogoro regions of eastern tanzania is reported. the major clinical findings were severe respiratory distress, fever, mucopurulent nasal discharge and high mortality involving all age groups and both sexes of goats. the morbidity and mortality rates were 45%-90% and 14%-50%, respectively. the principal pathological lesions were confined to the thoracic cavity and comprised hydrothorax and serofibrinous pleuropneu ... | 2000 | 11126580 |
| detection of specific mycoplasma conjunctivae antibodies in the sera of sheep with infectious keratoconjunctivitis. | the serological cross reactions between mycoplasma conjunctivae, the etiological agent of infectious keratoconjunctivitis (ikc), and the antigenetically and phylogenetically closely related mycoplasma ovipneumoniae, which is often found in sheep, were analysed. cross reacting antigens were identified using sera from sheep with ikc and from sheep of herds known to be free of ikc, as well as rabbit hyperimmune serum specific to the two mycoplasma species. cross reactions were predominantly due to ... | 2001 | 11361151 |
| detection of mycoplasma ovipneumoniae in pasteurella-vaccinated sheep flocks with respiratory disease in england. | | 2003 | 14682543 |
| mycoplasma species and related organisms isolated from ruminants in britain between 1990 and 2000. | between 1990 and 2000, more than 1600 mycoplasmas and the related acholeplasmas were identified from ruminant animals by the mycoplasma group at the veterinary laboratories agency--weybridge. mycoplasma bovis was the most commonly identified pathogen, mostly from pneumonic calves but occasionally from cattle with mastitis and arthritis. mycoplasma canis was first isolated in britain in 1995 from pneumonic calves and the number of isolates increased to 18 per cent of the total mycoplasmas isolate ... | 2004 | 15508840 |
| chronic non-progressive pneumonia of sheep in new zealand - a review of the role of mycoplasma ovipneumoniae. | chronic non-progressive pneumonia (cnp) is a common disease which affects lambs in new zealand during late summer and autumn. mycoplasma ovipneumoniae can be recovered from a high proportion of lesions but it is also present in some normal lungs. bacteria, especially pasteurella haemolytica, can also be recovered from more than half the lungs of affected animals. isolates of m. ovipneumoniae are genetically heterogeneous, as demonstrated by examination of their dna or total cellular proteins, an ... | 1999 | 16032095 |
| new transtracheal bronchoalveolar lavage technique for the diagnosis of respiratory disease in sheep. | a new transtracheal bronchoalveolar lavage technique for the diagnosis of respiratory disease in sheep under field conditions was tested in 76 sheep. the sheep were divided into three groups, normal sheep, sheep with clinical signs of respiratory disease and housed sheep, on the basis of their respiratory disease history and husbandry conditions. the detection of mannheimia haemolytica and mycoplasma ovipneumoniae or parainfluenza virus type 3 and bovine respiratory syncytial virus antigen in th ... | 2005 | 16155238 |
| use of nasal swabs in diagnosis of respiratory disease. | | 2006 | 16582002 |
| an aetiopathological study of chronic bronchopneumonia in lambs in ireland. | chronic bronchopneumonia in lambs, also known as 'atypical' or 'chronic, non-progressive' pneumonia is a common, frequently sub-clinical disease affecting animals under 12-months-old in intensive production systems. infection with both mycoplasma ovipneumoniae and mannheimia haemolytica have been implicated in the aetiology of this condition and a variety of pulmonary lesions can result. in this study, detailed laboratory examination of 30 abattoir-derived lungs with the characteristic gross fea ... | 2007 | 16632391 |
| a high level of strain variation within the mycoplasma ovipneumoniae population of the uk has implications for disease diagnosis and management. | mycoplasma ovipneumoniae is one of only two mycoplasma species associated with small ruminant disease in britain and has been associated with an increasing number of disease outbreaks since 2002. this investigation used well-defined techniques to assess the variability of uk m. ovipneumoniae isolates, in an attempt to identify strain clusters within the population. strains received for routine diagnosis between 2002 and 2004 were analysed using random amplified polymorphic dna (rapd) and pulsed ... | 2006 | 16962728 |
| isolation and immunological detection of mycoplasma ovipneumoniae in sheep with atypical pneumonia, and lack of a role for mycoplasma arginini. | mycoplasma ovipneumoniae nctc 10151(t) and four new isolates from uk sheep flocks were compared. only glucose and pyruvate were used as energy sources by the five strains: glucose was the best energy source for the type strain, pyruvate supported better growth of the new strains. whole cell protein patterns and antigenic profiles showed high similarity between all five strains. the new isolates fell into two groups in elisa tests. serum samples from 30 pneumonic sheep were assessed for m. ovipne ... | 2008 | 17662318 |
| a novel medium devoid of ruminant peptone for high yield growth of mycoplasma ovipneumoniae. | mycoplasma ovipneumoniae is considered an emerging veterinary pathogen causing pneumonia in sheep and goats worldwide. currently it has not been possible to define a growth medium that yields the maximum growth of m. ovipneumoniae within a short incubation period. growth yields of m. ovipneumoniae in eaton's medium are variable and not as consistently high as those seen with other mycoplasma spp. this study investigated the ability of different m. ovipneumoniae field strains to grow in various m ... | 2008 | 17980518 |
| association of mycoplasma ovipneumoniae infection with population-limiting respiratory disease in free-ranging rocky mountain bighorn sheep (ovis canadensis canadensis). | bronchopneumonia is a population-limiting disease in bighorn sheep in much of western north america. previous investigators have isolated diverse bacteria from the lungs of affected sheep, but no single bacterial species is consistently present, even within single epizootics. we obtained high-quality diagnostic specimens from nine pneumonic bighorn sheep in three populations and analyzed the bacterial populations present in bronchoalveolar lavage specimens of seven by using a culture-independent ... | 2008 | 18057131 |
| microorganisms associated with a pneumonic epizootic in rocky mountain bighorn sheep (ovis canadensis canadensis). | a comprehensive study of a pneumonic epizootic was initiated when the first signs of disease were noted in a metapopulation of bighorn sheep inhabiting hells canyon, bordering idaho, oregon, and washington. a total of 92 bighorn sheep were tested for etiologic agents during the following 6-mo study period. the study population included bighorn sheep believed to be the subpopulation in which disease was first noted, and these sheep were translocated to a holding facility in an effort to contain t ... | 2007 | 18229860 |
| atypical non-progressive pneumonia in goats. | an outbreak of severe respiratory disease in a goat herd was associated with mycoplasma ovipneumoniae, mycoplasma arginini, mannheimia haemolytica and pasteurella multocida with mortality rates exceeding 20% in kids. post mortem features in affected kids included severe pleuropneumonia, lung consolidation, large quantities of pleural fluid and pericarditis. this is the first report of atypical proliferative pneumonia in goats in portugal. | 2010 | 19596209 |
| global suppression of mitogen-activated ovine peripheral blood mononuclear cells by surface protein activity from mycoplasma ovipneumoniae. | mycoplasma ovipneumoniae is associated with chronic non-progressive pneumonia of sheep and goats. as with many other mycoplasmas involved in animal diseases, protective immune responses have not been achieved with vaccines, even though antibody responses can be obtained. this study focuses on characterizing the interaction of m. ovipneumoniae with ovine pbmc using carboxy-fluorescein-succinimidyl-ester (cfse) loading and flow cytometry to measure lymphoid cell division. m. ovipneumoniae induced ... | 2010 | 20189658 |
| pneumonia of lambs in the abruzzo region of italy: anatomopathological and histopathological studies and localisation of mycoplasma ovipneumoniae. | the most common forms of inflammation of the lower respiratory tract in lambs are acute enzootic pneumonia, caused mainly by mannheimia haemolytica, chronic enzootic pneumonia (defined as 'atypical' in lambs), the aetiological of which is mycoplasma ovipneumoniae and viral inflammation principally caused by parainfluenza virus type 3. the authors conducted anatomopathological and histopathological studies of the most commonly encountered spontaneous lung inflammations in lambs slaughtered in the ... | 2007 | 20411506 |
| mycoplasma ovipneumoniae can predispose bighorn sheep to fatal mannheimia haemolytica pneumonia. | mycoplasma ovipneumoniae has been isolated from the lungs of pneumonic bighorn sheep (bhs). however experimental reproduction of fatal pneumonia in bhs with m. ovipneumoniae was not successful. therefore the specific role, if any, of m. ovipneumoniae in bhs pneumonia is unclear. the objective of this study was to determine whether m. ovipneumoniae alone causes fatal pneumonia in bhs, or predisposes them to infection by mannheimia haemolytica. we chose m. haemolytica for this study because of its ... | 2010 | 20466492 |
| a bighorn sheep die-off in southern colorado involving a pasteurellaceae strain that may have originated from syntopic cattle. | we investigated a pasteurellosis epizootic in free-ranging bighorn sheep (ovis canadensis) wherein a pasteurellaceae strain carried by syntopic cattle (bos taurus) under severe winter conditions appeared to contribute to pneumonia in affected bighorns. twenty-one moribund or dead bighorn sheep were found on the "fossil ridge" herd's winter range, colorado, usa, between 13 december 2007 and 29 february 2008. eight carcasses examined showed gross or microscopic evidence of acute to subacute fibrin ... | 2010 | 20966277 |
| molecular cloning of hsp70 in mycoplasma ovipneumoniae and comparison with that of other mycoplasmas. | mycoplasma ovipneumoniae, a bacterial species that specifically affects ovine and goat, is the cause of ovine infectious pleuropneumonia. we cloned, sequenced and analyzed heat shock protein 70 (hsp70) (dnak) gene of m. ovipneumoniae. the full length open reading frame of the m. ovipneumoniae hsp70 gene consists of 1812 nucleotides, with a g+c content of 34.16%, encoding 604 amino acids. comparative analysis with the hsp70 sequences of 15 mycoplasma species revealed 59 to 87% dna sequence identi ... | 2011 | 21574140 |
| mycoplasma ovipneumoniae associated with severe respiratory disease in goats. | | 2011 | 21610002 |
| genome sequence of mycoplasma ovipneumoniae strain sc01. | mycoplasma ovipneumoniae is associated with chronic nonprogressive pneumonia in both sheep and goats. studies concerning its molecular pathogenesis, genetic analysis, and vaccine development have been hindered due to limited genomic information. here, we announce the first complete genome sequence of this organism. | 2011 | 21742877 |
| seroepidemiological survey of sheep flocks from northern japan for mycoplasma ovipneumoniae and mycoplasma agalactiae. | sheep flocks from hokkaido, iwate and aomori, three northern prefectures of japan, were screened for antibodies to mycoplasma ovipneumoniae and mycoplasma agalactiae by elisa. sixty four animals out of 246 (26%) were seropositive to m. ovipneumoniae, with positive results obtained from all three prefectures. none of the sera tested were serologically positive to m. agalactiae. | 2011 | 21830171 |
| survival of bighorn sheep (ovis canadensis) commingled with domestic sheep (ovis aries) in the absence of mycoplasma ovipneumoniae. | to test the hypothesis that mycoplasma ovipneumoniae is an important agent of the bighorn sheep (ovis canadensis) pneumonia that has previously inevitably followed experimental commingling with domestic sheep (ovis aries), we commingled m. ovipneumoniae-free domestic and bighorn sheep (n=4 each). one bighorn sheep died with acute pneumonia 90 days after commingling, but the other three remained healthy for >100 days. this unprecedented survival rate is significantly different (p=0.002) from that ... | 2012 | 22247385 |
| [molecular typing and immunoblotting of 17 mycoplasma ovipneumoniae isolates from china]. | to study the heterogeneity and immunogenic variability among mycoplasma ovipneumoniae (m. ovipneumoniae) isolates from different regions of china. | 2011 | 22233065 |
| elongation factor tu and heat shock protein 70 are membrane-associated proteins from mycoplasma ovipneumoniae capable of inducing strong immune response in mice. | chronic non-progressive pneumonia, a disease that has become a worldwide epidemic has caused considerable loss to sheep industry. mycoplasma ovipneumoniae (m. ovipneumoniae) is the causative agent of interstitial pneumonia in sheep, goat and bighorn. we here have identified by immunogold and immunoblotting that elongation factor tu (ef-tu) and heat shock protein 70 (hsp 70) are membrane-associated proteins on m. ovipneumonaiea. we have evaluated the humoral and cellular immune responses in vivo ... | 2016 | 27537186 |
| mycoplasma ovipneumoniae--a primary cause of severe pneumonia epizootics in the norwegian muskox (ovibos moschatus) population. | the norwegian muskox (ovibos moschatus) population lives on the high mountain plateau of dovre and originates from animals introduced from greenland. in the late summers of 2006 and 2012, severe outbreaks of pneumonia with mortality rates of 25-30% occurred. during the 2012 epidemic high quality samples from culled sick animals were obtained for microbiological and pathological examinations. high throughput sequencing (pyrosequencing) of pneumonic lung tissue revealed high concentrations of myco ... | 2014 | 25198695 |
| safety and immunogenicity of a mycoplasma ovipneumoniae bacterin for domestic sheep (ovis aries). | mortality from epizootic pneumonia is hindering re-establishment of bighorn sheep populations in western north america. mycoplasma ovipneumoniae, a primary agent of this disease, is frequently carried asymptomatically by the domestic sheep and goats that constitute the reservoir of this agent for transmission to bighorn sheep. our long-term objective is to reduce the risk of m. ovipneumoniae infection of bighorn sheep; one approach to this objective is to control the pathogen in its reservoir ho ... | 2014 | 24752006 |
| mycoplasma ovipneumoniae induces sheep airway epithelial cell apoptosis through an erk signalling-mediated mitochondria pathway. | mycoplasma ovipneumoniae (m. ovipneumoniae) is a species of mycoplasma bacteria that specifically infects sheep and goat, causing ovine infectious pleuropneumonia. however, the mechanism underlying the pathogen-host interaction between m. ovipneumoniae and airway epithelial cells is unknown. | 2016 | 27663303 |
| assessing timing and causes of neonatal lamb losses in a bighorn sheep ovis canadensis canadensis herd via use of vaginal implant transmitters. | we evaluated the use of vaginal implant transmitters (vits) as a means of detecting, capturing, and radio collaring rocky mountain bighorn sheep ( ovis canadensis canadensis) lambs to estimate survival and to facilitate carcass recovery to assess causes of mortality. we focused on one of several bighorn herds in colorado, usa, suffering from depressed recruitment that was not preceded by a classic all-age die-off. we captured, radio-collared, diagnosed pregnancy by ultrasound examination, and in ... | 2017 | 28192042 |
| how respiratory pathogens contribute to lamb mortality in a poorly performing bighorn sheep ( ovis canadensis ) herd. | we evaluated bighorn sheep ( ovis canadensis ) ewes and their lambs in captivity to examine the sources and roles of respiratory pathogens causing lamb mortality in a poorly performing herd. after seven consecutive years of observed december recruitments of <10%, 13 adult female bighorn sheep from the remnant gribbles park herd in colorado, us were captured and transported to the thorne-williams wildlife research center in wyoming in march 2013. ewes were sampled repeatedly over 16 mo. in april ... | 2017 | 27690193 |
| fusobacterium necrophorum in north american bighorn sheep ( ovis canadensis ) pneumonia. | fusobacterium necrophorum has been detected in pneumonic bighorn sheep (bhs; ovis canadensis ) lungs, in addition to the aerobic respiratory pathogens mannheimia haemolytica , bibersteinia trehalosi , pasteurella multocida , and mycoplasma ovipneumoniae . similar to m. haemolytica , f. necrophorum produces a leukotoxin. leukotoxin-induced lysis and degranulation of polymorphonuclear leukocytes (pmns) and macrophages are responsible for acute inflammation and lung tissue damage characteristic of ... | 2016 | 27224212 |
| epizootic pneumonia of bighorn sheep following experimental exposure to mycoplasma ovipneumoniae. | bronchopneumonia is a population limiting disease of bighorn sheep (ovis canadensis). the cause of this disease has been a subject of debate. leukotoxin expressing mannheimia haemolytica and bibersteinia trehalosi produce acute pneumonia after experimental challenge but are infrequently isolated from animals in natural outbreaks. mycoplasma ovipneumoniae, epidemiologically implicated in naturally occurring outbreaks, has received little experimental evaluation as a primary agent of bighorn sheep ... | 2014 | 25302992 |
| pcr assay detects mannheimia haemolytica in culture-negative pneumonic lung tissues of bighorn sheep (ovis canadensis) from outbreaks in the western usa, 2009-2010. | mannheimia haemolytica consistently causes severe bronchopneumonia and rapid death of bighorn sheep (ovis canadensis) under experimental conditions. however, bibersteinia trehalosi and pasteurella multocida have been isolated from pneumonic bighorn lung tissues more frequently than m. haemolytica by culture-based methods. we hypothesized that assays more sensitive than culture would detect m. haemolytica in pneumonic lung tissues more accurately. therefore, our first objective was to develop a p ... | 2014 | 24171569 |
| causes of pneumonia epizootics among bighorn sheep, western united states, 2008-2010. | epizootic pneumonia of bighorn sheep is a devastating disease of uncertain etiology. to help clarify the etiology, we used culture and culture-independent methods to compare the prevalence of the bacterial respiratory pathogens mannheimia haemolytica, bibersteinia trehalosi, pasteurella multocida, and mycoplasma ovipneumoniae in lung tissue from 44 bighorn sheep from herds affected by 8 outbreaks in the western united states. m. ovipneumoniae, the only agent detected at significantly higher prev ... | 2012 | 22377321 |
| bighorn sheep pneumonia: sorting out the cause of a polymicrobial disease. | pneumonia of bighorn sheep (ovis canadensis) is a dramatic disease of high morbidity and mortality first described more than 80 years ago. the etiology of the disease has been debated since its initial discovery, and at various times lungworms, mannheimia haemolytica and other pasteurellaceae, and mycoplasma ovipneumoniae have been proposed as primary causal agents. a multi-factorial "respiratory disease complex" has also been proposed as confirmation of causation has eluded investigators. in th ... | 2013 | 23253148 |
| investigations into the seasonal presence of mycoplasma species in fattening lambs. | the presence of infection with mycoplasma species in association with lung consolidation, environmental temperature and relative humidity was investigated in 410 clinically healthy fattening lambs from five different feedlots in extremadura (southwestern spain). isolates of mycoplasma species were obtained (n= 117), including mycoplasma ovipneumoniae (n = 18) and mycoplasma arginini (n = 99). two seasonal periods were identified. the first period, which included february, march, september, octob ... | 2016 | 27256030 |
| studies into the prevalence of mycoplasma species in small ruminants in benue state, north-central nigeria. | the indicative prevalence of respiratory mycoplasma species in small ruminants (sr) was determined in north-central nigeria. nasal swabs from 172 sheep and 336 goats from the northeast, northwest and south senatorial districts of benue state were examined. initial mycoplasma isolation used mycoplasma culture techniques followed by digitonin sensitivity testing. species identification was done using polymerase chain reaction (pcr) and denaturing gradient gel electrophoresis (dgge). overall, mycop ... | 2014 | 24828562 |
| identification by culture, pcr, and immunohistochemistry of mycoplasmas and their molecular typing in sheep and lamb lungs with pneumonia in eastern turkey. | this study used cultures, polymerase chain reaction (pcr), and immunoperoxidase to examine samples from 216 lungs from sheep and lambs with macroscopic pneumonia lesions for the presence of mycoplasma species. dna was extracted from lung tissue samples and broth cultures with the help of a dna extraction kit and replicated using genus-specific and species-specific primers for mycoplasma. the lung samples were examined by the immunoperoxidase method using hyperimmune mycoplasma ovipneumoniae seru ... | 2013 | 23494576 |
| molecular characterization of the heat shock protein 70 gene in mycoplasma ovipneumoniae. | mycoplasma ovipneumoniae is a species of mycoplasma bacteria that commonly infects the respiratory tract, causing respiratory disease in sheep and goats worldwide. in the current study, the 70-kda heat shock protein (hsp70) gene was cloned, sequenced and analyzed in 14 clinical isolates of m. ovipneumoniae. results showed that, compared to the reference y98 strain, the open-reading frames (orfs) of hsp70 gene in all isolates were 1818 base pairs (bp). three nucleotides of tca were inserted at 1, ... | 2013 | 24011586 |
| capsular polysaccharide of mycoplasma ovipneumoniae induces sheep airway epithelial cell apoptosis via ros-dependent jnk/p38 mapk pathways. | in an attempt to better understand the pathogen-host interaction between invading mycoplasma ovipneumoniae (m. ovipneumoniae) and sheep airway epithelial cells, biological effects and possible molecular mechanism of capsular polysaccharide of m. ovipneumoniae (cps) in the induction of cell apoptosis were explored using sheep bronchial epithelial cells cultured in air-liquid interface (ali). the cps of m. ovipneumoniae was first isolated and purified. results showed that cps had a cytotoxic effec ... | 2017 | 28367270 |
| contact and contagion: bighorn sheep demographic states vary in probability of transmission given contact. | 1.understanding both contact and probability of transmission given contact are key to managing wildlife disease. however, wildlife disease research tends to focus on contact heterogeneity, in part because probability of transmission given contact is notoriously difficult to measure. here we present a first step toward empirically investigating probability of transmission given contact in free-ranging wildlife. 2.we used measured contact networks to test whether bighorn sheep demographic states v ... | 2017 | 28317104 |
| comparison of phenotypic and genotypic profiles among caprine and ovine mycoplasma ovipneumoniae strains. | mycoplasma ovipneumoniae (movp) is considered to be one of the most important mycoplasmas causing respiratory disease in small ruminants. most epidemiologic and characterisation studies have been conducted on strains collected from sheep. information on the presence and characteristics of movp in healthy and pneumonic goats is limited. phenotypic or genotypic differences between sheep and goat isolates have never been studied. the objective of our study was to characterise and compare the simila ... | 2017 | 27895290 |
| cloning and sequence analysis of wild argali splunc1 cdna. | the complete coding sequence of wild argali splunc1 cdna was generated by rapid amplification of cdna ends. the splunc1 cdna was 1076 bp with an open reading frame of 768 bp, which encoded a 26.49 kda protein composed of 255 amino acids. its amino acid sequence shared 98.4%, 96.9%, 94.5%, 90.2%, 80.8 %, 78.4%, 78.3%, 72.5%, 72.3%, 68.8% identity with those of splunc1 cdna from ovis aries (accession no. np_001288334.1), capra hircus (accession no. xp_005688516.1), pantholops hodgsonii (accession ... | 2016 | 27620892 |
| role of carriers in the transmission of pneumonia in bighorn sheep (ovis canadensis). | in the absence of livestock contact, recurring lamb mortality in bighorn sheep (ovis canadensis) populations previously exposed to pneumonia indicates the likely presence of carriers of pneumonia-causing pathogens, and possibly inadequate maternally derived immunity. to investigate this problem we commingled naïve, pregnant ewes (n=3) with previously exposed rams (n=2). post-commingling, all ewes and lambs born to them acquired pneumonia-causing pathogens (leukotoxin-producing pasteurellaceae an ... | 2016 | 27185269 |
| serological and molecular survey of sheep infected with mycoplasma ovipneumoniae in xinjiang, china. | mycoplasma pneumonia is one of the most important infectious diseases that threaten sheep production. in order to investigate the epidemic status of mycoplasma ovipneumoniae infection in sheep, indirect hemagglutination assay was used to analyze 1679 serum samples collected from four different breeds of sheep (kazak sheep, hu sheep, merino sheep, and duolang sheep) in six regions in xinjiang between 2012 and 2014. one thousand one hundred sixty-nine sheep nasal swabs and 180 lungs were pcr analy ... | 2015 | 26315151 |