Publications

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detection and differentiation of avian mycoplasmas by surface-enhanced raman spectroscopy based on silver nanorod array.mycoplasma gallisepticum is a bacterial pathogen of poultry that is estimated to cause annual losses exceeding $780 million. the national poultry improvement plan guidelines recommend regular surveillance and intervention strategies to contain m. gallisepticum infections and ensure mycoplasma-free avian stocks, but several factors make its detection and diagnosis a major challenge. current techniques are laborious, require special expertise, and are typically plagued by false results. in this st ...201122210215
cloning and analysis of the gene for a major surface antigen of mycoplasma gallisepticum.myplasma gallisepticum infects a wide variety of gallineaceous birds including chickens, turkeys, and pheasants. infection occurs both horizontally and vertically. thus, control of the spread of m. gallisepticum to noninfected flocks is difficult. continual monitoring is necessary to identify infected flocks even under the most stringent infectious control practices. monitoring, however, is usually performed by measuring hemagglutination activity (ha) in serum, an insensitive and variable test. ...200212495041
infectious agents associated with respiratory disease in pheasants.in a case-control study of the infectious agents associated with natural outbreaks of respiratory disease in pheasants, 28 batches of birds from sites affected by disease and eight batches of birds from unaffected sites were examined by six veterinary laboratories in england, wales and scotland, and tested for mycoplasmas, other bacteria and viruses. sinusitis was the commonest sign of disease and was associated with mycoplasma gallisepticum as detected by pcr in the trachea (p < 0.05) and conju ...200212054135
effects of dual infection of chickens with mycoplasma synoviae and mycoplasma gallinaceum or infectious bursal disease virus on infectious synovitis. 19836314027
immunohistochemical demonstration of mycoplasma gallinarum and mycoplasma gallinaceum in naturally infected hen oviducts.using indirect immunoperoxidase (iip), peroxidase anti-peroxidase (pap) and avidin biotin-peroxidase complex (abc) immunohistochemical methods, mycoplasma gallinarum and m gallinaceum antigens were demonstrated in ethanol-fixed paraffin-embedded oviduct sections from hens the eggs from which showed suboptimal hatchability. specific immunoperoxidase staining was detected at the mucosa in the magnum portion of the oviduct. optimal staining was achieved by applying the abc method, though both iip a ...19902267425
complete genome sequence of mycoplasma gallinaceum.mycoplasma gallinaceum strain b2096 8b was isolated from domestic chickens in south africa. the 845,307-bp full genome was sequenced, assembled, and annotated.201526139719
mycoplasmas and respiratory disease in pheasants and partridges.pheasants and partridges with signs of upper respiratory disease were cultured for mycoplasmas and were also examined for mycoplasma gallisepticum and mycoplasma synoviae using commercial polymerase chain reaction (pcr) kits. sixty-two incidents of disease were investigated in pheasants and 12 in partridges. m. gallisepticum was detected by culture in only four and three incidents in pheasants and partridges, respectively, but with pcr a further 15 m. gallisepticum-positive incidents were detect ...200119184924
infraorbital sinusitis associated with pasteurella multocida in pen-raised ring-necked pheasants.pasteurella multocida, somatic serotype 6, was isolated from the infraorbital sinuses of 8-wk-old ring-necked pheasants with severe sinusitis. in addition, escherichia coli, pasteurella haemolytica-like bacteria, mycoplasma gallinaceum, and mycoplasma glycophilum were also isolated from some of the sinuses. clinical signs appeared 3 days after placement on the grow-out ranch. the sinusitis consisted of severe unilateral or bilateral distention of the sinuses by mucoid to caseous exudate. mortali ...200111417842
the characterization of mycoplasma synoviae ef-tu protein and proteins involved in hemadherence and their n-terminal amino acid sequences.an abundant cytoplasmic 43-kda protein from mycoplasma synoviae, a major pathogen from poultry, was identified as elongation factor tu. the n-terminal amino acid sequence (akldfdrskehvnvgtighv) has 90% identity with the sequence of the mycoplasma hominis elongation factor tu protein. monoclonal antibodies reacting with the m. synoviae elongation factor tu protein also reacted with 43-kda proteins from the avian mycoplasma species mycoplasma gallinarum, mycoplasma gallinaceum, mycoplasma pullorum ...199910220885
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