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a field trial with a multicomponent inactivated respiratory viral vaccine.an inactivated virus vaccine containing strains of parainfluenza type 3 (pi3), bovine adenovirus type 3, reovirus type 1, bovine virus diarrhoea (bvd) and infectious bovine rhinotracheitis (ibr) viruses was tested in a group of 58 calves reared in a semi-intensive management system. following vaccination, 1/30, 14/30 and 17/30, showed significant rises in antibody titre to reovirus type 1, adenovirus type 3 and ibr respectively. none of the animals showed significant serological response to pi 3 ...1979232326
the incidence of mycoplasma dispar, ureaplasma and conventional mycoplasma in the pneumonic calf lung.this report describes the incidence of mycoplasma dispar, ureaplasma and conventional (large colony) mycoplasma isolated from the pneumonic lungs of groups of young calves and the identification to species level of mycoplasmas in mixed populations with the aid of the indirect fluorescent antibody test. pneumonic lung tissue yielded one or more mycoplasma species from 88% of the 153 calves cultured. the mycoplasmas identified and percent of the calves with lungs positive for each species were: m. ...1979398234
[intrauterine mycoplasmosis].comparative studies of pathological anatomy of intrauterine mycoplasmosis of man and cattle were carried out. on the basis of investigations of 22 deceased newborns and of 3 stillborns with uterine infection caused by mycoplasma pneumonia descriptions of lesions of various organs are presented. they most regularly were defected in the liver. in the respiratory organs in this infection, in contrast to respiratory mycoplasmosis, lesions of respiratory departments, not of the respiratory tract, wer ...1977558745
sensitivites in vitro to antimicrobial drugs of bovine mycoplasmas isolated from respiratory and genital tracts.a total of 155 mycoplasma strains were examined for sensitivity to nine antibiotics and four nitrofurans by the agar dilution method. they consisted of 69 strains of mycoplasma bovirhinis, 33 strains of m. bovigenitalium, 49 strains of acholeplasma laidlawii and four strains of a. modicum isolated from the nasal secretions, tracheas and lungs of calves manifesting respiratory symptoms and from bovine genital tracts collected at a slaughterhouse. as a result, furamizole and mitomycin c showed the ...1978652043
the recovery of a mycoplasma from citellus richardsonii richardsonii (ground squirrel).swabs from the upper respiratory tract, external genitalia and the eyes and portions of the lungs, spleen, kidney, liver and uteri of two ground squirrels were cultured for mycoplasma. the upper respiratory tracts of both animals were positive for mycoplasma as were the lungs, liver and spleen of one of the animals. preliminary serological studies, growth inhibition test against 38 known antisera, growth precipitation against 15 antisera and fluorescent antibody technique with eight conjugates h ...1977861842
pathogenicity of certain mycoplasma species in the bovine mammary gland.strains of three of the species of mycoplasmas isolated from pneumonic calf lungs, mycoplasma dispar, mycoplasma bovirhinis and acholeplasma laidlawii, were inoculated into the bovine mammary gland. pathogenicity of the species was monitored by counting the inflammatory cells and mycoplasmas in milk. six of the seven m dispar strains produced clinical mastitis, but the seventh strain, vic12, was apparently avirulent. the four strains of m bovirhinis caused subclinical mastitis. none of the four ...1976935661
mycoplasma species recovered from the reproductive tracts of western canadian cows.samples of cervico-vaginal mucus from 633 animals from 110 herds were cultured and yielded the following mycoplasmas: t-strain--88: mycoplasma bovigenitalium--79, mycoplasma spp. (leach group 7)--7, acholeplasma laidlawii--4, mycoplasma bovirhinis--2 and one not typable. uterine exudates and endometrial scrapings from 80 infertile cows in two herds were examined. four animals were positive, m. bovigenitalium was isolated three times, a. laidlawii and mycoplasma arginini once each. sixty-five nor ...19751125831
mycoplasmas and cuffing pneumonia in a group of calves.the mycoplasmas found in the lungs of 20 calves, housed together for six months, and the related pulmonary pathology are reported. twelve calves had cuffing pneumonia and in this group there was a significantly higher isolation frequency of mycoplasma dispar and ureaplasma spp compared with the non-pneumonic group. mycoplasma bovirhinis and acholeplasma laidlawii were isolated from the lungs of calves in both groups. mycoplasma arginini was not recovered from the lungs of any calf. the significa ...19751198879
[morphologic changes in the kidneys of calves with respiratory mycoplasmosis].the kidneys of 144 naturally diseased calves and those of 47 experimentally infected with mycoplasma bovirhinis calves were investigated by histological and immunomorphological methods. the kidneys of calves with respiratory mycoplasmosis were often found to be involved in the pathological process, and at the initial stage of the disease and at its height prolifirative, membranous-prolifirative glomerulonephritis, and at later stages proliferative-fibroplastic glomerulonephritis developed. the s ...19761275784
changes in the bacterial flora of the upper and lower respiratory tracts and bronchoalveolar lavage differential cell counts in feedlot calves treated for respiratory diseases.serial nasopharyngeal swab and bronchoalveolar lavage cultures were used to estimate changes in the bacterial flora of the respiratory tracts of calves during the first month after arrival in the feedlot. bronchoalveolar lavage (bal) differential cell counts served to evaluate pulmonary inflammatory changes during this period. two groups of calves were studied, one consisting of clinically normal controls (n = 60), the other, of cases (n = 59) which received treatment for respiratory disease (pe ...19921423052
isolation of mycoplasma bovirhinis from the kidneys of a bull with urinary obstruction and subacute nephritis.a bull with urethral obstruction secondary to urolithiasis died following exploratory laparotomy. postmortem examination revealed renal, cystic, and urethral calculi. gross and histologic findings were consistent with acute urethral and bilateral renal obstruction. bacteriologic culture of renal tissue revealed mycoplasma bovirhinis.19902391278
[associative interrelationship of mycoplasma bovirhinis and the infectious rhinotracheitis virus in vitro]. 19862855990
growth and interaction of mycoplasma bovirhinis and mycoplasma dispar in vitro.two mycoplasmas were grown in pure and mixed cultures in glucose calf-serum broth with initial ph 7.8. sensitivity to ph was also tested. the main data for mycoplasma bovirhinis and mycoplasma dispar, respectively, in pure cultures were as follows: lag phase, days: less than 1, 1-2; log phase: 1-2, 1-4; relatively stationary phase: 0-1, 2-4; decline phase (to the extent roughly logarithmic): 2-6, 5-10; maximal titers: 5 x 10(7) to 10(9), 5 x 10(6) to 10(8) color changing units per 0.2 ml; highes ...19873322657
autoregulation of b cell growth by an immunoglobulin m autoantibody.this study documents the autoregulation of b cell growth by an igm autoantibody. this autoantibody is secreted by b lymphocytes upon stimulation by polyclonal activators and is identified by its potent inhibition of b cell growth induced by these agents (endotoxin, fc fragment of human gamma-globulin, mycoplasma bovirhinis, and anti-mouse ig). the autoantibody specifically affects b cells: there was no effect on mitogen- or antigen-induced t cell proliferation and of responses to interleukin 1, ...19853874225
effect of mycoplasma dispar, mycoplasma bovirhinis, acholeplasma laidlawii and t-mycoplasmas on explant cultures of bovine trachea. 19744604399
an enzootic of calf pneumonia associated with mycoplasma bovirhinis. 19734799971
adsorbing and non-adsorbing strains of mycoplasma bovirhinis. 19715132801
prevalence of mycoplasma and chlamydia in the nasal flora of dairy calves.nasal swabs from dairy calves in 20 selected herds were collected once in each of three sampling periods in one year and cultured for mycoplasmas and chlamydiae. seventy-seven of 411 calves (19%) and 16 of 20 herds (80%) were positive for mycoplasmas at least once throughout the year, and all of 378 calves sampled from the 20 herds were negative for chlamydiae. thirty-one percent of the isolates were mycoplasma bovirhinis; other species isolated less frequently were mycoplasma bovis, mycoplasma ...19827179720
[pathogenic properties of mycoplasma bovirhinis]. 19817253978
failure to produce pneumonia in calves by inhalation of mycoplasma bovirhinis and ureaplasma aerosols. 19817304368
aminopeptidase activity of acholeplasma laidlawii, mycoplasma bovirhinis, mycoplasma dispar and mycoplasma bovis.aminopeptidase activity was demonstrated in extracts of acholeplasma laidlawii, mycoplasma bovirhinis, m. bovis and m. dispar. the enzyme specificity, which differed amongst the mycoplasma species examined, was characterized using 19 aminoacyl-beta-naphthylamides as substrates. the differences were detected after 4 h incubation with the substrates and potentially could be employed to aid in the characterization of certain mycoplasma species.19807411115
a model for respiratory syncytial virus (rsv) infection based on experimental aerosol exposure with bovine rsv in calves.five conventionally kept calves aged between 17 and 24 days were experimentally infected with bovine respiratory syncytial virus (brsv) by aerosol in order to mimic the natural infection route. the calves were killed and autopsies performed 7 days after the first virus challenge. the brsv isolate used induced tracheitis, bronchitis and atelectasis in infected calves. the only virus which could be isolated from the lungs of the calves was brsv. in addition, mycoplasma bovirhinis was isolated from ...19968814979
phylogeny of some mycoplasmas from ruminants based on 16s rrna sequences and definition of a new cluster within the hominis group.almost complete (> 96%) 16s rrna sequences from nine ruminant mycoplasmas have been determined by solid-phase dna sequencing. polymorphisms were found in four of the 16s rrna sequences, which indicated the existence of two different rrna operons. seven polymorphisms were found in mycoplasma agalatiae, three were found in mycoplasma bovis, one was found in mycoplasma alkalescens, and one was found in mycoplasma bovirhinis. the sequence data were used for construction of phylogenetic trees. all bu ...19968863441
in vitro amplification of the 16s rrna genes from mycoplasma bovirhinis, mycoplasma alkalescens and mycoplasma bovigenitalium by pcr.polymerase chain reaction (pcr) systems were developed for detection of 3 pathogenic bovine mycoplasmas, mycoplasma alkalescens (mak), m. bovigenitalium (mbg) and m. bovirhinis (mbr). the primers were selected from the sequences of the 16s rrna from each of the mycoplasmas. neither the mbg-pcr system nor the mbr-pcr system showed cross-amplification among 24 bovine, caprine-ovine and human mycoplasma species (including acholeplasma and ureaplasma species) tested. the mak-pcr system showed cross- ...19989879529
biochemical characterization of mycoplasma bovirhinis, mycoplasma dispar and recent bovine isolates of mycoplasma canis.the pattern and kinetics of substrate utilization by the type strains of mycoplasma canis, m. bovirhinis and m. dispar and ten recent m. canis isolates from cattle were determined. metabolism of a range of sugars and organic acids by m. dispar was detectable by measurement of oxygen uptake. organic acids were not utilized by m. bovirhinis or m. canis, and there was no oxygen uptake during metabolism of glucose or other sugars, as monitored by a ph-change method. the m. canis strains varied in th ...200111214667
isolation of mycoplasma species from the lower respiratory tract of healthy cattle and cattle with respiratory disease in belgium.between 1997 and 2000, a total of 150 healthy cattle and 238 animals with respiratory disease were examined for six mycoplasma species. attempts were made to detect mycoplasma canis, mycoplasma dispar and ureaplasma diversum in calves with recurrent disease, and all three of these species were identified in calves with recurrent disease and in healthy lungs. in healthy calves, 84 per cent of bronchoalveolar lavage fluids were mycoplasma free; when cultures were positive, mycoplasma bovirhinis wa ...200212418530
isolation of mycoplasmas from nasal swabs of calves affected with respiratory diseases and antimicrobial susceptibility of their isolates.nasal swabs of 293 calves were examined for mycoplasma. the samples were collected from calves affected with respiratory diseases on 71 farms in various parts of japan between 1996 and 1997. mycoplasma bovirhinis was isolated from 47 of 293 calves (16.0%). mycoplasma alkalescens, m. bovis, m. arginini, m. bovigenitalium and acholeplasma spp. were isolated from 19 (6.5%), seven (2.4%), four (1.4%), four (1.4%) and 18 (6.1%) calves, respectively. pasteurella multocida and p. haemolytica were isola ...200314535934
characterization of a point mutation in the parc gene of mycoplasma bovirhinis associated with fluoroquinolone resistance.quinolone-resistant (qr) mutants of mycoplasma bovirhinis strain pg43 (type strain) were generated by stepwise selection in increasing concentrations of enrofloxacin (enr). an alteration was found in the quinolone resistance-determining region (qrdr) of the parc gene coding for the parc subunit of topoisomerase iv from these mutants, but not in the gyra, gyrb, and pare gene coding for the gyra and gyrb subunits of dna gyrase and the pare subunit of topoisomerase iv. similarly, such an alteration ...200415228551
mycoplasma species and related organisms isolated from ruminants in britain between 1990 and 2000.between 1990 and 2000, more than 1600 mycoplasmas and the related acholeplasmas were identified from ruminant animals by the mycoplasma group at the veterinary laboratories agency--weybridge. mycoplasma bovis was the most commonly identified pathogen, mostly from pneumonic calves but occasionally from cattle with mastitis and arthritis. mycoplasma canis was first isolated in britain in 1995 from pneumonic calves and the number of isolates increased to 18 per cent of the total mycoplasmas isolate ...200415508840
gelatinolytic matrix metalloproteinases-2 and -9 in tracheobronchial lavage fluid obtained from calves with concurrent infections of pasteurella multocida and mycoplasma bovirhinis.to study gelatinolytic matrix metalloproteinases (mmps) in tracheobronchial lavage fluid (tblf) obtained from clinically normal calves and calves with pasteurella multocida infection.200516379654
respiratory disease in calves: microbiological investigations on trans-tracheally aspirated bronchoalveolar fluid and acute phase protein response.trans-tracheal aspirations from 56 apparently healthy calves and 34 calves with clinical signs of pneumonia were collected in six different herds during september and november 2002. the 90 samples were cultivated and investigated by pcr tests targeting the species histophilus somni, mannheimia haemolytica, pasteurella multocida, mycoplasma bovis, mycoplasma dispar, and mycoplasma bovirhinis. a pcr test amplifying the lktc-artj intergenic region was evaluated and shown to be specific for the two ...200919186010
molecular epidemiological analysis of mycoplasma bovis isolates from the pennsylvania animal diagnostic laboratory showing genetic diversity.we have examined the genetic variability of mycoplasma bovis strains submitted to the pennsylvania animal diagnostics laboratory, university park (pa-adl), between december 2007 and december 2008. of 4,868 total samples submitted for mycoplasma testing, 302 were determined to be culture positive. mycoplasma bovis (63.6%), mycoplasma californicum (7.3%), mycoplasma bovirhinis (2.7%), mycoplasma bovigenitalium (0.7%), mycoplasma alkalescens (4.9%), mycoplasma putrefaciens (0.3%), and mycoplasma di ...201121426978
validation of a mycoplasma molecular diagnostic test and distribution of mycoplasma species in bovine milk among new york state dairy farms.mycoplasma mastitis is a contagious and costly disease of dairy cattle that significantly affects animal health and milk productivity. mycoplasma bovis is the most prevalent and invasive agent of mycoplasma mastitis in dairy cattle, and early detection is critical. other mycoplasma have been isolated from milk; however, the role and prevalence of these species as mastitis pathogens are poorly understood. routine screening of milk for mycoplasma by bacteriological culture is an important componen ...201627016831
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