| flavobacterium chilense sp. nov., and flavobacterium araucananum sp. nov., two novel species isolated from farmed salmonid in chile. | three gram-negative non endospore-forming filamentous, lm-09-fpt and lm-19-fpt, lm-20-fp, were isolated from rainbow trout (oncorhynchus mykiss) and atlantic salmon (salmo salar), respectively, and were studied in detail for their taxonomic position. 16s rrna gene sequence analysis showed that the three isolates clearly belong to the genus flavobacterium. isolate lm-09-fpt was most closely related to flavobacterium chungangense, showing 98.5% gene sequence similarity to the type strain of this s ... | 2011 | 21828008 |
| microbial degradation of validamycin a by flavobacterium saccharophilum. enzymatic cleavage of c-n linkage in validoxylamine a. | the enzymatic cleavage of c-n linkage in the degradation of validamycin a by flavobacterium saccharophilum was examined using n-p-nitrophenyl derivatives of validamine and valienamine as synthetic model substrates for validoxylamine a. incubation of n-p-nitrophenylvalidamine with the membrane fraction from the organism led to formation of n-p-nitrophenyl-3-ketovalidamine, and succeeding cleavage of c-n linkage. as the products of the cleavage step, one was identified as p-nitroaniline and anothe ... | 1984 | 6548220 |
| purification and properties of 3-ketovalidoxylamine a c-n lyase from flavobacterium saccharophilum. | 3-ketovalidoxylamine a c-n lyase was purified about 900-fold from the cell-free extract of flavobacterium saccharophilum by ammonium sulfate fractionation, column chromatography on cm cellulose and gel filtration on sephacryl s-200. the purified enzyme was homogeneous as judged by sodium dodecyl sulfate (sds) polyacrylamide gel electrophoresis. the molecular weight of the enzyme was estimated to be 36,000 by gel filtration on sephacryl s-200 and by sds polyacrylamide gel electrophoresis, indicat ... | 1985 | 4093450 |
| purification and properties of glucoside 3-dehydrogenase from flavobacterium saccharophilum. | a membrane-bound glucoside 3-dehydrogenase [ec 1.1.99.13], which oxidizes validoxylamine a to the 3-keto derivative, was solubilized from the membrane fraction of flavobacterium saccharophilum by triton x-100 and purified about 280-fold with an overall yield of 30% from the membrane fraction by column chromatography on deae- and cm-sepharose cl-6b and gel filtration on sephacryl s-300. the purified enzyme exhibited a single protein band on disc gel electrophoresis, and fad was shown to be the pr ... | 1986 | 3818559 |
| chemical modification by diethylpyrocarbonate of an essential histidine residue in 3-ketovalidoxylamine a c-n lyase. | 3-ketovalidoxylamine a c-n lyase of flavobacterium saccharophilum is a monomeric protein with a molecular weight of 36,000. amino acid analysis revealed that the enzyme contains 5 histidine residues and no cysteine residue. the enzyme was inactivated by diethylpyrocarbonate (dep) following pseudo-first order kinetics. upon treatment of the inactivated enzyme with hydroxylamine, the enzyme activity was completely restored. the difference absorption spectrum of the modified versus native enzyme ex ... | 1986 | 3745136 |
| physiological role of glucoside 3-dehydrogenase and cytochrome c551 in the sugar oxidizing system of flavobacterium saccharophilum. | flavobacterium saccharophilum cytoplasmic membranes contain several cytochromes linked to the respiratory chain. the presence of c-type cytochrome, cytochrome o, and a small amount of a-type cytochrome was proved. cytochrome c551 was purified to electrophoretic homogeneity by ion-exchange chromatography and gel filtration from a membrane fraction of f. saccharophilum and its properties determined. cytochrome c551 possessed absorption peaks at 407 nm in the oxidized form, and at 415, 521, 551 nm ... | 1988 | 2844746 |
| new cyclitols, degradation of validamycin a by flavobacterium saccharophilum. | | 1980 | 7251495 |
| new intermediates, degradation of validamycin a by flavobacterium saccharophilum. | | 1981 | 7328066 |
| flavobacterium aquidurense sp. nov. and flavobacterium hercynium sp. nov., from a hard-water creek. | ten new flavobacterium-like strains were isolated from freshwater of the hard-water creek westerhöfer bach, northern germany. these strains formed two phylogenetic groups: strains wb 1.1-56t, wb 1.1-04, wb 1.1-14, wb 1.1-57 and wb 1.1-63; and strains wb 4.2-33t, wb 4.1-86, wb 4.2-34, wb 4.2-32 and wb 4.2-78. cells were gram-negative, yellow-pigmented, chemoheterotrophic rods. their major fatty acid profiles were similar, consisting of iso-c(15 : 0), iso-c(15 : 0) 3-oh, iso-c(17 : 0) 3-oh and sum ... | 2007 | 17267958 |
| chemical modification by 2,4,6-trinitrobenzenesulfonic acid (tnbs) of an essential amino group in 3-ketovalidoxylamine a c-n lyase. | 3-ketovalidoxylamine a c-n lyase of flavobacterium saccharophilum is a monomeric protein with a molecular weight of 36000, and contains 32 amino groups and no cysteine or cystine residues. the enzyme was inactivated by 2,4,6-trinitrobenzenesulfonic acid (tnbs) following pseudo-first order kinetics. substrate of the lyase, p-nitrophenyl-3-ketovalidamine, protected the enzyme against the inactivation, suggesting that the modification occurred at or near the active site. although several amino grou ... | 1990 | 2393971 |
| preparation of 3-amino-3-deoxy derivatives of trehalose and sucrose and their activities. | the 3-keto derivatives of trehalose and sucrose respectively were prepared by a one-step enzymatic route using d-glucoside 3-dehydrogenase from flavobacterium saccharophilum. reductive amination of 3-ketotrehalose with ammonium acetate and sodium cyanoborohydride gave three compounds, 3-amino-3-deoxy-alpha-d-allopyranosyl alpha-d-glucopyranoside, 3-amino-3-deoxy-alpha-d-glucopyranosyl alpha-d-glucopyranoside (3-trehalosamine) and 3-amino-3-deoxy-alpha-d-mannopyranosyl alpha-d-glucopyranoside. fr ... | 1989 | 2498271 |