| genetic characterization of pathogenic leptospira species by dna hybridization. | a total of 66 serovars of potentially pathogenic leptospira species were examined by slot blot hybridization, and 57 of these serovars were classified in six dna homology groups. in cases in which common serovars were studied, the results were in general agreement with the results of previous workers, who used different dna homology methods. however, we propose a new species, leptospira kirschneri, comprising the following serovars: bulgarica, butembo, cynopteri, dania, grippotyphosa, kabura, ka ... | 1992 | 1581182 |
| further determination of dna relatedness between serogroups and serovars in the family leptospiraceae with a proposal for leptospira alexanderi sp. nov. and four new leptospira genomospecies. | dna relatedness was determined among 303 strains of leptospira and leptonema. included in the analysis were reference strains from 228 well-characterized and recognized serovars. the study included 268 serovars from 29 named and one or more unnamed serogroups. the strains clustered into 17 dna hybridization groups, representing 12 previously described species (292 strains) and five new genomospecies (11 strains). the largest groups included leptospira interrogans (91 strains from 82 serovars), l ... | 1999 | 10319510 |
| analysis of leptospira isolates from mainland portugal and the azores islands. | from 228 recent leptospira isolates from mainland portugal and azorean wild mammals, 149 were characterized at the serovar level by monoclonal antibodies (mabs), a quick serological method in epidemiological studies. in order to compare this antigenic information with that from new genetic techniques, a sample of isolates was analyzed through pulsed-field agarose gel electrophoresis (pfge) (n=71), mapped restriction site polymorphisms (mrsps) in pcr-amplified rrna genes (n=45, including 13 sapro ... | 2000 | 10754245 |
| leptospira infection in two black rhinoceroses (diceros bicornis michaeli). | two black rhinoceroses (diceros bicornis michaeli) developed clinical leptospirosis without hemolytic crises. the first rhinoceros presented with peracute depression, anorexia, rear leg trembling, dysuria, glucosuria, gastrointestinal discomfort, and decreased fecal output and died within 12 hr. necropsy and histopathology revealed lesions within multiple organs. leptospirosis was diagnosed postmortem based on positive fluorescent antibody staining of liver. the second rhinoceros presented 2 mo ... | 2001 | 12785703 |
| serologic responses of dogs given a commercial vaccine against leptospira interrogans serovar pomona and leptospira kirschneri serovar grippotyphosa. | to evaluate serum titers obtained by use of the microscopic agglutination test (ie, mat titers) to leptospira interrogans serovar pomona and autumnalis and leptospira kirschneri serovar grippotyphosa in dogs given a commercial vaccine against serovars pomona and grippotyphosa. | 2005 | 16273911 |
| leptospirosis in squirrels imported from united states to japan. | we diagnosed leptospirosis in 2 patients exposed to southern flying squirrels imported from the united states to japan. patients worked with exotic animals in their company. leptospira isolates from 1 patient and 5 of 10 squirrels at the company were genetically and serologically identical and were identified as leptospira kirschneri. | 2006 | 16836840 |
| application of multilocus variable-number tandem-repeat analysis for molecular typing of the agent of leptospirosis. | leptospirosis is a worldwide-distributed zoonosis, endemic in tropical areas. epidemiologic investigations of leptospirosis still rely on tedious serological identification tests. recently, molecular typing systems based on variable-number tandem-repeat (vntr) analysis have been described and have been used to identify leptospira interrogans strains. although l. interrogans is the most common leptospira species encountered in human infections around the world, other pathogenic species, such as l ... | 2006 | 17088367 |
| identification of leptospires of the pomona and grippotyphosa serogroups isolated from cattle in zimbabwe. | two strains of the genus leptospira, isolated from kidneys of oxen slaughtered in zimbabwe, one belonging to serogroup pomona (strain sbf 8) and the other to serogroup grippotyphosa (strain sbf 32), were identified by using cross-agglutinin absorption, monoclonal antibody, restriction fragment length polymorphism and polymerase chain reaction analyses. the identification of the two strains was equivocal. strain sbf 8 showed a close similarity to both serovars mozdok and proechimys by cross-agglu ... | 1995 | 8525094 |
| dna relatedness of leptospira strains isolated from beef cattle in zimbabwe. | the dna relatedness of 17 leptospira strains isolated from beef cattle in zimbabwe was determined using the hydroxyapatite method. similarly to previously speciated african strains, all zimbabwe isolates belonged to either leptospira borgpetersenii or leptospira kirschneri. all serovars within serogroups pyrogenes (kwale, mombe and a strain closely related to serovar nigeria), hebdomadis (marondera and mhou), tarassovi (ngavi) and sejroe (balcanica and hardjo) were l. borgpetersenii. l. kirschne ... | 1999 | 10425768 |
| sequence analysis and characterization of a 40-kilodalton borrelia hermsii glycerophosphodiester phosphodiesterase homolog. | we report the purification, molecular cloning, and characterization of a 40-kda glycerophosphodiester phosphodiesterase homolog from borrelia hermsii. the 40-kda protein was solubilized from whole organisms with 0.1% triton x-100, phase partitioned into the triton x-114 detergent phase, and purified by fast-performance liquid chromatography (fplc). the gene encoding the 40-kda protein was cloned from a b. hermsii chromosomal dna lambda exlox expression library and identified by using affinity an ... | 1997 | 9079909 |
| molecular detection of leptospira kirschneri in tissues of a prematurely born foal. | leptospirosis was identified to be the possible cause of premature birth in a foal on a farm with a history of repeated abortions. using an appropriate polymerase chain reaction (pcr) assay, the presence of leptospira kirschneri was detected in the tissues of the prematurely born foal. further confirmation of l. kirschneri was obtained by nucleotide sequence analysis of the pcr-amplified dna fragment and the partial 16s ribosomal rna gene sequence. this report further supports mounting evidence ... | 2005 | 15690955 |
| clinical and pathologic comparison of acute leptospirosis in dogs caused by two strains of leptospira kirschneri serovar grippotyphosa. | to develop a method for inducing acute leptospirosis in dogs. | 2004 | 15334844 |
| evaluation of environmental risk factors for leptospirosis in dogs: 36 cases (1997-2002). | to identify environmental risk factors for leptospirosis. | 2004 | 15239476 |
| serovar-specific prevalence and risk factors for leptospirosis among dogs: 90 cases (1997-2002). | to estimate serovar-specific prevalence of leptospirosis by use of veterinary teaching hospital and laboratory submission data; describe annual and seasonal patterns of leptospirosis; and identify risk factors for age, sex, and breed. | 2004 | 15230451 |
| an improved immunohistochemical diagnostic technique for canine leptospirosis using antileptospiral antibodies on renal tissue. | the purpose of this study was to compare the immunoreactivity in canine renal tissues stained with antisera specific for 3 leptospiral antigens and those processed with traditional staining methods. in addition, immunoglobulin staining was done on tissues with immunoreactivity to leptospiral antigens. formalin-fixed renal sections from 12 dogs with chronic interstitial nephritis suspected or proven to have leptospirosis (6 dogs with silver-stained leptospires and 6 dogs in which silver-stained l ... | 2002 | 12680639 |
| low-stringency single specific primer pcr for identification of leptospira. | thirty-five leptospira serovars from the species leptospira interrogans, leptospira borgpetersenii, leptospira santarosai, leptospira kirschneri, leptospira weilii, leptospira biflexa and leptospira meyeri were characterized by the low-stringency single specific primer pcr (lssp-pcr) technique. lssp-pcr analysis was performed to detect dna polymorphisms in a 285 bp dna fragment amplified from genomic dna with g1 and g2 selected primers. similar lssp-pcr profiles were obtained for serovars from t ... | 2003 | 12543918 |
| novel 45-kilodalton leptospiral protein that is processed to a 31-kilodalton growth-phase-regulated peripheral membrane protein. | leptospiral protein antigens are of interest as potential virulence factors and as candidate serodiagnostic and immunoprotective reagents. we identified leptospiral protein antigens by screening a genomic expression library with serum from a rabbit hyperimmunized with formalin-killed, virulent leptospira kirschneri serovar grippotyphosa. genes expressing known outer membrane lipoproteins lipl32 and lipl41, the heat shock protein groel, and the alpha, beta, and beta' subunits of rna polymerase we ... | 2002 | 11748198 |
| the leptospiral major outer membrane protein lipl32 is a lipoprotein expressed during mammalian infection. | we report the cloning of the gene encoding the 32-kda lipoprotein, designated lipl32, the most prominent protein in the leptospiral protein profile. we obtained the n-terminal amino acid sequence of a staphylococcal v8 proteolytic-digest fragment to design an oligonucleotide probe. a lambda-zap ii library containing ecori fragments of leptospira kirschneri dna was screened, and a 5.0-kb dna fragment which contained the entire structural lipl32 gene was identified. several lines of evidence indic ... | 2000 | 10722630 |
| diagnosis of canine leptospirosis by a highly sensitive fret-pcr targeting the lig genes. | canine leptospirosis is underdiagnosed due to its wide spectrum of clinical presentations and the lack of a rapid and sensitive test for the accurate diagnosis of acute and chronic infections. in this study, we developed a highly sensitive and specific fluorescence resonance energy transfer (fret)-pcr to detect common pathogenic leptospires in dogs, including leptospira interrogans serovars autumnalis, canicola, copenhageni (icterohaemorrhagiae serogroup) and pomona, and leptospira kirschneri se ... | 2014 | 24586833 |
| leptospiral outer membrane proteins ompl1 and lipl41 exhibit synergistic immunoprotection. | new vaccine strategies are needed for prevention of leptospirosis, a widespread human and veterinary disease caused by invasive spirochetes belonging to the genus leptospira. we have examined the immunoprotective capacity of the leptospiral porin ompl1 and the leptospiral outer membrane lipoprotein lipl41 in the golden syrian hamster model of leptospirosis. specialized expression plasmids were developed to facilitate expression of leptospiral proteins in escherichia coli as the membrane-associat ... | 1999 | 10569777 |
| identification of a serogroup bataviae leptospira strain isolated from an ox in zimbabwe. | a strain belonging to the genus leptospira serogroup bataviae, isolated from an ox at slaughter in zimbabwe, was identified by using cross-agglutinin absorption, the monoclonal antibody, restriction fragment length polymorphism, and polymerase chain reaction analyses. results of both serological tests used showed that the isolate (strain sbf 37) was antigenically similar to reference strain paidjan and therefore belongs to serovar paidjan. strain sbf 37 was, however, genetically different from s ... | 1999 | 10096163 |
| expression and distribution of leptospiral outer membrane components during renal infection of hamsters. | the outer membrane of pathogenic leptospira species grown in culture media contains lipopolysaccharide (lps), a porin (ompl1), and several lipoproteins, including lipl36 and lipl41. the purpose of this study was to characterize the expression and distribution of these outer membrane antigens during renal infection. hamsters were challenged with host-derived leptospira kirschneri to generate sera which contained antibodies to antigens expressed in vivo. immunoblotting performed with sera from ani ... | 1999 | 9916100 |
| characterization of leptospiral outer membrane lipoprotein lipl36: downregulation associated with late-log-phase growth and mammalian infection. | we report the cloning of the gene encoding a 36-kda leptospiral outer membrane lipoprotein, designated lipl36. we obtained the n-terminal amino acid sequence of a staphylococcal v8 proteolytic-digest fragment in order to design an oligonucleotide probe. a lambda-zap ii library containing ecori fragments of leptospira kirschneri dna was screened, and a 2.3-kb dna fragment which contained the entire structural lipl36 gene was identified. several lines of evidence indicate that lipl36 is lipid modi ... | 1998 | 9529084 |
| [research on the recombinant plasmid pdjh2 of l. interrogans serovar lai: sequencing and alignment with other known bacterial omp sequence]. | the leptospira whole cell vaccine (lwcv) currently used in china is safe and effective, out the immunity following vaccination with two doses of the fluid medium vaccine is of low order. the duration of immunity conferred by this vaccine is rather short, six months or at most one year. therefore, it is necessary to develop new generation vaccines against leptospirosis for the developing world. in this paper we report the sequencing of the insert fragment of pdjh2 from genomic dna of l. interroga ... | 1996 | 9389001 |
| detection of pathogenic leptospira bacteria in pinniped populations via pcr and identification of a source of transmission for zoonotic leptospirosis in the marine environment. | leptospirosis, caused by the spirochete leptospira, is a geographically widespread disease that affects a broad range of mammals, including marine mammals. among pinniped populations, periodic epizootics of leptospirosis are responsible for significant die-offs. along the west coast of north america, the most recent leptospirosis epizootic occurred in 2004, during which samples were collected from cases ranging from california to british columbia. the primary objective of this study was to use t ... | 2008 | 18367568 |
| neglected leptospirosis in raccoons (procyon lotor) in indiana, usa. | leptospirosis is a globally important zoonotic disease occurring clinically and subclinically in humans and animals. | 2014 | 24784701 |
| an attempt to control a natural focus of leptospirosis grippotyphosa by rodenticide--a long-term study (1977-2004). | the purpose of the study was to suppress a known natural focus of field fever exerting an influence on animal hosts of leptospires--small terrestrial mammals (s.t.m.) by rodenticide. after repeated application of the zinc phosphide rodenticide, the s.t.m. were regularly live-trapped and checked for leptospirosis by dark-field microscopy and culture of their renal tissue and serological examination. isolated leptospira strains were typed by help of rabbit factor sera. the deratization influenced ... | 2007 | 17655177 |
| molecular typing of leptospira spp. based on putative o-antigen polymerase gene (wzy), the benefit over 16s rrna gene sequence. | molecular typing of leptospiral strains based on variation within putative o-antigen polymerase gene (wzy) was determined among reference strains and those isolated from patients. using the pcr primers designed from the flanking gene of wzy derived from leptospira interrogans serovar copenhageni, all l. interrogans serovars as well as human and rodent leptospiral isolates from thailand could be amplified. the size of pcr product ranged from 1 to 1.5 kb. the limitation of these primer pairs was t ... | 2007 | 17403048 |
| development of species-specific pcr primer sets for the detection of leptospira. | spirochetes of the genus leptospira infect animals and humans and are the causative agents for the emerging infectious disease leptospirosis. rapid and simple assays for the identification of individual leptospira species are currently not available. for identification of individual leptospira species, pcr primers that detect the ompl1 gene sequence for the majority of pathogenic leptospires were developed in this study. the primer pairs detect leptospira interrogans, leptospira borgpetersenii, ... | 2006 | 17020546 |
| evaluation of lssp-pcr for identification of leptospira spp. in urine samples of cattle with clinical suspicion of leptospirosis. | we evaluated the use of low-stringency single specific primer pcr (lssp-pcr) for genetically typing leptospira directly from urine samples of cattle with clinical suspicion of leptospirosis. urine samples obtained from 40 cattle with clinical suspicion of leptospirosis were amplified by specific pcr using the following primers: internal 1/internal 2 and g1/g2. the internal primers were designed from the gene sequence of the outer membrane lipoprotein lip32 from leptospira kirschneri, strain rm52 ... | 2006 | 16962259 |
| new leptospira serovar sokoine of serogroup icterohaemorrhagiae from cattle in tanzania. | the prevalence of leptospirosis is generally high in domestic animals and rodents in tanzania. identification of leptospira isolates from cattle was carried out to establish prevalent leptospira serovars. serological typing was done based on monoclonal antibodies and the standard cross-agglutination absorption test. molecular typing involved pathogenic- and saprophytic-specific pcrs and a pcr specifically amplifying dna from the species leptospira kirschneri. dna fingerprinting with primers deri ... | 2006 | 16514033 |
| evaluation of recombinant lig antigen-based elisa for detection of leptospiral antibodies in canine sera. | abstract. the objectives of this study were to clone the conserved region of leptospiral immunoglobulin-like protein (lig) gene and evaluate the utility of the recombinant lig as an elisa antigen for detection of leptospiral antibodies in canine sera. leptospira kirschneri serovar grippotyposa strain moskva v was chosen to be a target for cloning the conserved region of lig gene. this assay was evaluated with canine sera (n = 91) that were mat-negative (< 1:100 dilution) and sera (n = 103) that ... | 2011 | 21323175 |
| electrocardiographic changes in hospitalized patients with leptospirosis over a 10-year period. | the aim of this study was to investigate the incidence and type of ecg changes in patients with leptospirosis regardless of clinical evidence of cardiac involvement. | 2011 | 21709630 |
| effects of recent leptospira vaccination on whole blood real-time pcr testing in healthy client-owned dogs. | bacterin-based canine leptospira vaccines could present a challenge for the use of whole blood real-time polymerase chain reaction (pcr) as a diagnostic tool. recent vaccination could induce positive results if the targeted dna fragment is present within the vaccine and in the blood of the recently vaccinated dog. | 2011 | 22182214 |
| leptospire genomic diversity revealed by microarray-based comparative genomic hybridization. | comparative genomic hybridization was used to compare genetic diversity of five strains of leptospira (leptospira interrogans serovars bratislava, canicola, and hebdomadis and leptospira kirschneri serovars cynopteri and grippotyphosa). the array was designed based on two available sequenced leptospira reference genomes, those of l. interrogans serovar copenhageni and l. interrogans serovar lai. a comparison of genetic contents showed that l. interrogans serovar bratislava was closest to the ref ... | 2012 | 22344655 |
| regional differences of leptospirosis in sri lanka: observations from a flood-associated outbreak in 2011. | leptospirosis is known to be an important cause of weather disaster-related infectious disease epidemics. in 2011, an outbreak of leptospirosis occurred in the relatively dry district of anuradhapura, sri lanka where diagnosis was resisted by local practitioners because leptospirosis was not known in the area and the clinical presentation was considered atypical. to identify the causative leptospira associated with this outbreak, we carried out a cross-sectional study. consecutive clinically sus ... | 2014 | 24454971 |
| [molecular cloning and expression in e. coli of the surface-exposed lipoprotein lipl41 gene of leptospira lai]. | to construct a recombinant expressive plasmid using lipl41 gene of leptospira lai for further research of subunit vaccine of leptospira. | 2001 | 12536557 |
| characterization of the leptospiral outer membrane and description of three novel leptospiral membrane proteins. | the outer membrane (om) of the mammalian pathogen leptospira kirschneri was isolated in the form of membrane vesicles by alkaline plasmolysis and separated from the protoplasmic cylinder by sucrose density gradient ultracentrifugation. all four components of the alkaline plasmolysis buffer, including 1.0 m nacl, 27% sucrose (wt/vol), 2 mm edta, and 10 mm tris (ph 9), were required for efficient om release, as judged by recovery of leptospiral lipopolysaccharide. two populations of om vesicles (o ... | 2002 | 12183539 |
| molecular analysis of leptospira spp. isolated from humans by restriction fragment length polymorphism, real-time pcr and pulsed-field gel electrophoresis. | a total of 17 leptospira clinical strains isolated from humans in croatia were serologically and genetically analysed. for serovar identification, the microscopic agglutination test (mat) and pulsed-field gel electrophoresis (pfge) were used. to identify isolates on genomic species level, pcr-based restriction fragment length polymorphism (rflp) and real-time pcr were performed. mat revealed the following serogroup affinities: grippotyphosa (seven isolates), icterohaemorrhagiae (eight isolates) ... | 2009 | 19780841 |
| multilocus sequence analysis for typing leptospira interrogans and leptospira kirschneri. | fifty-three strains belonging to the pathogenic species leptospira interrogans and leptospira kirschneri were analyzed by multilocus sequence analysis. the species formed two distinct branches. in the l. interrogans branch, the phylogenetic tree clustered the strains into three subgroups. genogroups and serogroups were superimposed but not strictly. | 2010 | 19955271 |
| molecular cloning and sequence analysis of the gene encoding lipl41, a surface-exposed lipoprotein of pathogenic leptospira species. | we report the cloning of the gene encoding a surface-exposed leptospiral lipoprotein, designated lipl41. in a previous study, a 41-kda protein antigen was identified on the surface of leptospira kirschneri (d. a. haake, e. m. walker, d. r. blanco, c. a. bolin, j. n. miller, and m. a. lovett, infect. immun. 59:1131-1140, 1991). we obtained the n-terminal amino acid sequence of a staphylococcal v8 proteolytic-digest fragment in order to design an oligonucleotide probe.a lambda zap ii library conta ... | 1996 | 8675344 |
| a simple and rapid nested polymerase chain reaction-restriction fragment length polymorphism technique for differentiation of pathogenic and nonpathogenic leptospira spp. | a rapid and specific nested polymerase chain reaction-restriction fragment length polymorphism (pcr-rflp) has been developed to detect and differentiate pathogenic and nonpathogenic leptospira spp. leptospiral genomic dna was extracted from suspected human sera using an improved method of standard phenol-chloroform, and specific primers have been used to amplify 16s ribosomal rna from all pathogenic and nonpathogenic leptospira spp. the pcr products of all nonpathogenic species were digested wit ... | 2009 | 19097839 |
| leptospira interrogans serogroup pomona infections in the uk: is there a real threat for farm animals? | current typing of leptospira strains requires a combination of both serological and molecular methods. study of the epidemiology of strains within the pomona serogroup has proved problematic due to the limitations of serological typing methods. this is particularly true of the two main complexes within this group, namely the pomona, monjakov, kennewicki cluster in leptospira interrogans species and the mozdok, tsaratsovo, kunming, altodouro cluster in leptospira kirschneri strains from the south ... | 2017 | 28213528 |
| molecular characterisation and disease severity of leptospirosis in sri lanka. | leptospirosis is a re-emerging zoonotic disease all over the world, important in tropical and subtropical areas. a majority of leptospirosis infected patients present as subclinical or mild disease while 5-10% may develop severe infection requiring hospitalisation and critical care. it is possible that several factors, such as the infecting serovar, level of leptospiraemia, host genetic factors and host immune response, may be important in predisposition towards severe disease. different leptosp ... | 2015 | 26061234 |
| similarities in leptospira serogroup and species distribution in animals and humans in the indian ocean island of mayotte. | our objective was to identify local animal reservoirs of leptospirosis to explain the unusual features of leptospira strains recently described among patients on the island of mayotte. by means of a microscopic agglutination test using local clinical isolates, we found that 11.2% of black rats were seropositive to leptospira, whereas 10.2% of flying foxes, 2% of lemurs, 93.1% of domestic dogs, and 87.5% of stray dogs were seropositive. as observed in humans, mini was the main serogroup circulati ... | 2012 | 22764304 |
| identification of tenrec ecaudatus, a wild mammal introduced to mayotte island, as a reservoir of the newly identified human pathogenic leptospira mayottensis. | leptospirosis is a bacterial zoonosis of major concern on tropical islands. human populations on western indian ocean islands are strongly affected by the disease although each archipelago shows contrasting epidemiology. for instance, mayotte, part of the comoros archipelago, differs from the other neighbouring islands by a high diversity of leptospira species infecting humans that includes leptospira mayottensis, a species thought to be unique to this island. using bacterial culture, molecular ... | 2016 | 27574792 |
| comparative genomic analysis of brazilian leptospira kirschneri serogroup pomona serovar mozdok. | leptospira kirschneri is one of the pathogenic species of the leptospira genus. human and animal infection from l. kirschneri gained further attention over the last few decades. here we present the isolation and characterisation of brazilian l. kirschneri serogroup pomona serovar mozdok strain m36/05 and the comparative genomic analysis with brazilian human strain 61h. the m36/05 strain caused pulmonary hemorrhagic lesions in the hamster model, showing high virulence. the studied genomes present ... | 2016 | 27581124 |
| human leptospirosis in tanzania: sequencing and phylogenetic analysis confirm that pathogenic leptospira species circulate among agro-pastoralists living in katavi-rukwa ecosystem. | leptospirosis is a neglected zoonotic disease of worldwide public health importance. the disease affects humans, domestic animals and wildlife. however, leptospirosis is challenging in its diagnosis in humans. culture technique, which is time consuming, is not recommended for clinical diagnosis. for these reasons, serological and molecular techniques remain the test of choice. the major objective of this study was to explore the genetic characteristic of leptospira species which are prevalent am ... | 2016 | 27287703 |
| etiological agents causing leptospirosis in sri lanka: a review. | to systematically review the etiological agent causing human leptospirosis in sri lanka. | 2016 | 27086159 |
| molecular epidemiology of pathogenic leptospira spp. in the straw-colored fruit bat (eidolon helvum) migrating to zambia from the democratic republic of congo. | the role played by bats as a potential source of transmission of leptospira spp. to humans is poorly understood, despite various pathogenic leptospira spp. being identified in these mammals. here, we investigated the prevalence and diversity of pathogenic leptospira spp. that infect the straw-colored fruit bat (eidolon helvum). we captured this bat species, which is widely distributed in africa, in zambia during 2008-2013. we detected the flagellin b gene (flab) from pathogenic leptospira spp. i ... | 2015 | 25791930 |
| evolution of the rnase p rna structural domain in leptospira spp. | we have employed the rnase p rna (rpr) gene, which is present as single copy in chromosome i of leptospira spp. to investigate the phylogeny of structural domains present in the rna subunit of the trna processing enzyme, rnase p. rpr gene sequences of 150 strains derived from ncbi database along with sequences determined from 8 reference strains were examined to fathom strain specific structural differences present in leptospiral rpr. sequence variations in the rpr gene impacted on the configura ... | 2014 | 25463388 |
| prevalence of antibody to six leptospira servovars in swedish wild boars. | zoonotic leptospira bacteria are pathogens that may increase in importance with climate change. we investigated the prevalence of antibody to six leptospira serovars (sv) in the swedish wild boar (sus scrofa) population, which is increasing in number and geographic distribution. the serovars we selected cause disease in pigs or may be of use as sentinel serovars to measure the potential spread in swedish fauna. in total, 386 serum samples from wild boars collected between 2005 and 2007 were inve ... | 2012 | 22493129 |
| serologic survey and results of urinary pcr testing for leptospirosis in captive black-tailed prairie dogs (cynomys ludovicianus). | leptospirosis is an important zoonotic disease occurring clinically and subclinically in humans and a wide variety of mammal species worldwide. often, rodents and wild animals are identified as important reservoirs for the disease. twenty-two captive black-tailed prairie dogs (cynomys ludovicianus) housed within a zoo were examined as part of a routine census and preventive medicine program. during examinations, blood and urine were collected to screen for exposure to, or infection with, leptosp ... | 2015 | 26667541 |
| isolation and clinical sample typing of human leptospirosis cases in argentina. | leptospira typing is carried out using isolated strains. because of difficulties in obtaining them, direct identification of infective leptospira in clinical samples is a high priority. multilocus sequence typing (mlst) proved highly discriminatory for seven pathogenic species of leptospira, allowing isolate characterization and robust assignment to species, in addition to phylogenetic evidence for the relatedness between species. in this study we characterized leptospira strains circulating in ... | 2016 | 26658064 |
| redefining gold standard testing for diagnosing leptospirosis: further evidence from a well-characterized, flood-related outbreak in sri lanka. | a gap in the leptospirosis field remains the lack of well-characterized sample collections that allow for comparison of new methods to standard ones. in the context of a flood-related outbreak of leptospirosis evaluated in anuradhapura, sri lanka, a specimen bank was obtained with detailed metadata accompanied by gold standard diagnostic test results. blood samples collected on admission and 14 days later from suspected cases of leptospirosis were tested using microscopic agglutination test (mat ... | 2016 | 27402521 |
| infection with leptospira kirschneri serovar mozdok: first report from the southern hemisphere. | leptospirosis is a global zoonosis caused by pathogenic leptospira spp. in this study, we characterized two leptospira kirschneri serogroup pomona serovar mozdok isolates, one obtained from a dog and the other from a patient with severe leptospirosis, 4 years later. histopathological analysis showed that both isolates caused severe tissue damage when used to infect hamsters. while l. kirschneri serogroup pomona serovar mozdok is endemic in animals in europe, there is only one report of human lep ... | 2016 | 26755566 |
| molecular characterization and serology of leptospira kirschneri (serogroup grippotyphosa) isolated from urine of a mare post-abortion in brazil. | a strain of leptospira kirschneri (serogroup grippotyphosa) was cultured from urine of a mare post-abortion in brazil and characterized by serogrouping, multiple-locus variable-number tandem repeat analysis, pgfe, and sequencing of genes rrs and secy. strains of l. kirschneri have apparently never been recovered from horses in tropical area, only in europe and usa. knowledge of local epidemiology is important to interpret genetic profiles of leptospires circulating in an area. | 2016 | 26355500 |
| human and animal leptospirosis in southern brazil: a five-year retrospective study. | leptospirosis is an emerging zoonosis attributed to multiple reservoirs. climatic conditions influence the transmission of pathogenic leptospires, which require warm and humid conditions for survival. the influence of seasonality in human and animal leptospirosis in the subtropical region of brazil remains poorly understood. | 2017 | 28743546 |
| molecular and serological characterization of leptospira kirschneri serogroup pomona isolated from a human case in a brazilian rural area. | leptospirosis is an important health concern in brazil. currently, information on the epidemiology of the disease in the rural areas of the country is lacking. | 2017 | 28700061 |
| rapid maldi-tof mass spectrometry identification of leptospira organisms. | leptospirosis is a worldwide deadly zoonotic disease. accurate identification of the causative leptospira spp. spirochetes ascertains the pathogenic status of the isolates, identifies potential source of infection and recognises outbreaks. species identification is currently based on technically demanding, time and resources consuming serological and molecular methods. matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof-ms) recently emerged as a first-line met ... | 2012 | 22386673 |
| variable-number tandem-repeat analysis of leptospiral dna isolated from canine urine samples molecularly confirmed to contain pathogenic leptospires. | objective to use variable-number tandem-repeat (vntr) analysis to determine the infecting serovar and strain for leptospiral dna isolated from canine urine samples confirmed through pcr testing to contain pathogenic leptospires and to evaluate the sensitivity and specificity of microscopic agglutination testing (mat) for identifying the infecting serogroup. design diagnostic survey and test evaluation. sample leptospiral dna isolated from urine samples from 98 dogs confirmed through pcr testing ... | 2016 | 27479284 |
| altodouro, a new leptospira serovar of the pomona serogroup isolated from rodents in northern portugal. | a leptospira strain (designated rim 139) was isolated from the kidney of a house-mouse, mus musculus, in trás-os-montes e alto douro region of northern portugal. the isolate showed typical leptospiral motility and morphology under dark-field microscopy and was pathogenic for hamsters. species determination was carried out on basis of pcr products generated by species-specific primers and by sequencing of the secy gene. putative serogroup typing was performed using the microscopic agglutination t ... | 2013 | 23070280 |