| granulocytic ehrlichiosis in a roe deer calf in norway. | a case of granulocytic ehrlichiosis is described in a roe deer (capreolus capreolus) calf from norway. the calf was heavily infested with ixodes ricinus and died from escherichia coli septicemia. granulocytic ehrlichia sp. was detected by polymerase chain reaction (pcr) from several organs and sequence determination identified a variant of human granulocytic ehrlichiosis (hge) agent. this is the first report of a possible clinical granulocytic ehrlichia sp. infection in a roe deer. | 2001 | 11504236 |
| detection of ehrlichial infection by pcr in dogs from yamaguchi and okinawa prefectures, japan. | species-specific nested polymerase chain reaction (pcr) was used to detect the presence of possible canine ehrlichial agents (ehrlichia canis, e. chaffeensis, e. ewingii, e. equi and e. platys) and monocytic ehrlichial agents found in japan (e. muris and a recently discovered ehrlichia species detected from ixodes ovatus) in blood samples from dogs in yamaguchi and okinawa prefecture, japan. partial sequence of e. platys was detected from 1 of 67 dogs (1.5%) tested from yamaguchi prefecture and ... | 2001 | 11503912 |
| ehrlichiosis in a moose calf in norway. | a case of granulocytic ehrlichiosis in a moose calf (alces alces) in norway is described. the animal was heavily infested with ticks (ixodes ricinus), and died from a klebsiella pneumoniae septicemia. examination of blood smears from the calf revealed cytoplasmic inclusions (morulae) typical of infection with ehrlichia phagocytophila in the granulocytes. ehrlichia sp. was detected by polymerase chain reaction (pcr) in blood from the calf, and in the ticks. sequence determination identified it as ... | 2001 | 11272499 |
| [ultrastructural identification of ehrlichia sp in an experimentally infected dog in venezuela]. | this study is the first report made in venezuela concerning the ultrastructural characteristics of ehrlichia sp in mononuclear blood cells from an experimentally infected dog. the animal developed clinical manifestations characteristic of the infection, and typical intracitoplasmic inclusion bodies were clearly seen in blood smears stained with modified giemsa examined by light microscopy. microorganisms were visualized by transmission electron microscopy. the cytoplasmic inclusions, consisted o ... | 1999 | 10531750 |
| relationship between tick bites and the seroprevalence of babesia microti and anaplasma phagocytophila (previously ehrlichia sp.) in blood donors. | tick-borne diseases, particularly babesiosis and ehrlichiosis, represent recently emerging infections. despite an increased recognition of the threat tick-borne agents pose to blood safety, our understanding of the prevalence and transmissibility of these agents in blood donors is limited. | 2002 | 12473139 |
| infection with agents of human granulocytic ehrlichiosis, lyme disease, and babesiosis in wild white-footed mice (peromyscus leucopus) in connecticut. | white-footed mice, peromyscus leucopus, were captured in southern connecticut during 1997 and 1998 to determine the prevalence of infections caused by granulocytic ehrlichia sp., borrelia burgdorferi, and babesia microti. of the 50 mice captured and recaptured, 25 of 47 (53.2%) and 23 of 48 (47.9%) contained antibodies to the bds or nch-1 ehrlichia strains, respectively, as determined by indirect fluorescent antibody (ifa) staining methods. the majority (83.3%) of 48 mice also contained antibodi ... | 1999 | 10449470 |
| human granulocytic ehrlichiosis agent and ehrlichia chaffeensis reside in different cytoplasmic compartments in hl-60 cells. | the human granulocytic ehrlichiosis (hge) agent resides and multiplies exclusively in cytoplasmic vacuoles of granulocytes. double immunofluorescence labeling was used to characterize the nature of the hge agent replicative inclusions and to compare them with inclusions containing the human monocytic ehrlichia, ehrlichia chaffeensis, in hl-60 cells. although both ehrlichia spp. can coinfect hl-60 cells, they resided in separate inclusions. inclusions of both ehrlichia spp. were not labeled with ... | 1999 | 10024584 |
| experimental infection of lambs with an equine granulocytic ehrlichia species resembling the agent that causes human granulocytic ehrlichiosis (hge). | five lambs were inoculated with a granulocytic ehrlichia species originally isolated from a swedish horse with granulocytic ehrlichiosis (ege). the 16s rrna gene sequence of the swedish ehrlichia sp. causing ege was identical to the sequence of the agent causing human granulocytic ehrlichiosis (hge). after the inoculation, infected neutrophils and a low serologic response were seen in all lambs, but no clinical symptoms were observed. in one lamb 17% of the neutrophils were infected without a co ... | 1998 | 9926463 |
| different organisms associated with heartwater as shown by analysis of 16s ribosomal rna gene sequences. | cowdria ruminantium is a rickettsial parasite which causes heartwater, a economically important disease of domestic and wild ruminants in tropical and subtropical africa and parts of the caribbean. because existing diagnostic methods are unreliable, we investigated the small-subunit ribosomal rna (srrna) gene from heartwater-infected material to characterise the organisms present and to develop specific oligonucleotide probes for polymerase chain reaction (pcr) based diagnosis. dna was obtained ... | 1997 | 9299697 |
| deer ticks (ixodes scapularis) and the agents of lyme disease and human granulocytic ehrlichiosis in a new york city park. | rodent trapping and drag sampling in van cortlandt park, new york city, yielded all stages of ixodes scapularis, the deer tick vector of lyme disease and human granulocytic ehrlichiosis (hge). polymerase chain reaction analyses of the ticks showed borrelia burgdorferi and the ehrlichia sp. that causes hge. | 1997 | 9284380 |
| development and use of specific polymerase reaction for the detection of an organism resembling ehrlichia sp. in white-tailed deer. | the role of white-tailed deer (odocoileus virginianus) in the epidemiology of ehrlichia chaffeensis and the agent of human granulocytic ehrlichiosis (hge) is not fully understood, and diagnostic procedures may be complicated by the recent detection of 16s rdna sequence from an ehrlichia sp.-like organism in wild deer. a specific forward primer (dga) and an ehrlichia spp. reverse primer (ga1ur) were constructed to amplify this new, distinct ehrlichia sp.-like 16s rdna. the dga primer, a forward p ... | 1997 | 9131554 |
| characterization of the sf agent, an ehrlichia sp. isolated from the fluke stellantchasmus falcatus, by 16s rrna base sequence, serological, and morphological analyses. | the organism designated the sf agent was originally isolated in japan in 1962 from stellantchasmus falcatus metacercaria parasitic on gray mullet fish. the sf agent resembles members of the genus ehrlichia morphologically and exhibits weak antigenic cross-reactivity with ehrlichia sennetsu. this organism causes mild clinical signs in dogs, but severe splenomegaly and lymphadenopathy in mice. this suggests that the sf agent may be similar to either neorickettsia helminthoeca, an intracellular par ... | 1996 | 8573488 |
| in vitro proliferation of a canine granulocytic ehrlichia. | canine granulocytic ehrlichia sp., an agent which parasitizes the neutrophilic leukocytes in dogs, was transiently propagated in vitro. dogs were experimentally inoculated with blood containing canine granulocytic ehrlichia. bacteremias in experimentally infected dogs varied from 1.2 to 9.3% granulocytes infected. granulocytes from experimentally infected dogs were harvested and cultured in the presence of rpmi 1640 medium supplemented with fetal bovine serum, conditioned medium, and hepes buffe ... | 1993 | 8506608 |
| pharmacokinetics and tolerance of weekly oxygent ca infusions in the dog. | this study determined the oxygent ca (90% w/v perflubron emulsion, alliance pharmaceutical corporation) dose necessary to achieve a 3-4% fluorocrit, and the tolerance of this dose administered once per week for four weeks to dogs. this study simulated oxygent ca use as a radiosensitizing agent. six adult dogs were administered 6 ml/kg oxygent ca once per week for 4 weeks. blood samples were collected following infusion, until fluorocrits were < or = 0.5%. one week after the fourth infusion, thre ... | 1994 | 7849917 |
| recent developments in the molecular biology of anaplasmosis. | recent applications of dna analysis, cloning, sequencing and expression technology have resulted in significant advances in our understanding of the hemoparasite anaplasma marginale. analysis of 16s ribosomal rna has confirmed a phylogenetic position close to ehrlichia sp. and cowdria ruminantium. intact genomic dna of a. marginale digested with sfii separates into bands from 14 to 170 kbp on pulse-field gels, with a total genome size of 1200-1260 kbp and g + c content of 56 mol%. major surface ... | 1995 | 7597790 |
| enterocolitis caused by ehrlichia sp. in the horse (potomac horse fever). | potomac horse fever was reproduced in 15 ponies by transfusion of whole blood originally from two natural cases and subsequently from ponies infected by the transfusions. incubation periods varied from 9 to 15 days. affected ponies developed varying degrees of fever, diarrhea, anorexia, depression, and leukopenia. eleven affected ponies were killed, three died in the acute phase of the disease, and one did not show clinical signs. the most consistent post-mortem findings were fluid contents in t ... | 1986 | 3750739 |
| isolation and characterization of an ehrlichia sp. from a patient diagnosed with human ehrlichiosis. | a new disease was recognized in the united states in 1986. the etiologic agent, although not previously isolated from a human, appeared to be serologically related to ehrlichia canis, a canine leukotropic rickettsia. we obtained blood specimens from 27 febrile patients with a history of tick exposure. leukocytes from 24 patients not treated with tetracycline were placed onto a monolayer of dh82 cells. we performed indirect immunofluorescence on sera from all 27 febrile patients as well as sera f ... | 1991 | 1757543 |
| analyses of ehrlichia canis and a canine granulocytic ehrlichia infection. | ehrlichia canis and canine granulocytic ehrlichia sp. (cge) infect canine monocytes and granulocytes, respectively. e. canis has been cultured in vitro and used to develop an immunofluorescence assay. cge has not been cultured, and a serologic assay is not available. the sera of dogs infected with cge were reported to react with e. canis by immunofluorescence. in this study, the temporal response of immunoglobulin g (igg) was determined by an enzyme-linked immunosorbent assay (elisa) with purifi ... | 1992 | 1734046 |
| a case of human ehrlichiosis acquired in mali: clinical and laboratory findings. | we report on the clinical, epidemiologic, and laboratory characteristics of the first case of human ehrlichiosis acquired outside the united states caused by an ehrlichia sp. other than e. sennetsu. the patient, a 24-year-old woman, presumably acquired the infection in mali in northern africa; the diagnosis was made when she returned to north america. the patient reported a fever and diarrhea a week before she left mali; the diarrhea resolved, but the fever and chills continued. she also reporte ... | 1992 | 1539750 |
| a cluster of tick-borne infections: association with military training and asymptomatic infections due to rickettsia rickettsii. | during the spring of 1989, 86 members of a military unit from the state of maryland, usa, participated in two-week-long training manoeuvres in the states of arkansas (location fc) and virginia (location faph). acute febrile illnesses due to infections with two tick-borne pathogens, rickettsia rickettsii and ehrlichia sp., were confirmed serologically in 2 initial cases who were admitted to the hospital. a seroepidemiological investigation among unit members found an additional 17 of 109 individu ... | 1992 | 1412666 |
| aortic valve endocarditis in a dog due to bartonella clarridgeiae. | we report the first documented case of endocarditis associated with bartonella clarridgeiae in any species. b. clarridgeiae was identified as a possible etiological agent of human cat scratch disease. infective vegetative valvular aortic endocarditis was diagnosed in a 2.5-year-old male neutered boxer. historically, the dog had been diagnosed with a systolic murmur at 16 months of age and underwent balloon valvuloplasty for severe valvular aortic stenosis. six months later, the dog was brought t ... | 2001 | 11574571 |
| citrate synthase gene sequence: a new tool for phylogenetic analysis and identification of ehrlichia. | the sequence of the citrate synthase gene (glta) of 13 ehrlichial species (ehrlichia chaffeensis, ehrlichia canis, ehrlichia muris, an ehrlichia species recently detected from ixodes ovatus, cowdria ruminantium, ehrlichia phagocytophila, ehrlichia equi, the human granulocytic ehrlichiosis [hge] agent, anaplasma marginale, anaplasma centrale, ehrlichia sennetsu, ehrlichia risticii, and neorickettsia helminthoeca) have been determined by degenerate pcr and the genome walker method. the ehrlichial ... | 2001 | 11526124 |
| serologic and molecular evidence of coinfection with multiple vector-borne pathogens in dogs from thailand. | forty-nine dogs from thailand were evaluated for serologic evidence of exposure or polymerase chain reaction (pcr) evidence of infection with vectorborne pathogens, including ehrlichia sp. (ehrlichia canis, ehrlichia chaffeensis, ehrlichia equi, and ehrlichia risticii), bartonella vinsonii subsp. berkhoffi (bvb), spotted fever group (sfg) rickettsiae (rickettsia rickettsii), typhus group (tg) rickettsiae (rickettsia canada, rickettsia prowazekii, and rickettsia typhi), and babesia sp. (babesia c ... | 2001 | 11596732 |
| exploitation of interleukin-8-induced neutrophil chemotaxis by the agent of human granulocytic ehrlichiosis. | the agent of human granulocytic ehrlichiosis (hge) is an obligate intracellular bacterium with a tropism for neutrophils; however, the mechanisms of bacterial dissemination are not yet understood. interleukin-8 (il-8) is a chemokine that induces neutrophil migration to sites of infection for host defense against pathogens. we now show that hge bacteria, and the hge-44 protein, induce il-8 secretion in a promyelocytic (hl-60) cell line that has been differentiated along the neutrophil lineage wit ... | 2001 | 11500432 |
| [first detection of ehrlichiosis detected serologically and with the polymerase chain reaction in patients with borreliosis in the czech republic]. | infectious complications caused by genus ehrlichia were confirmed when samples taken from patients with lyme borreliosis with non-characteristic development and those sent directly by physician who treated the patient were examined. laboratory diagnostics of ehrlichiosis was based on hematological examination of blood, on anamnestic and epidemiological data, on indirect immunofluorescence serology verified using western blot test with specific antigen and by determination of dna agents using pol ... | 2001 | 11347209 |
| [molecular tools in the epidemiology of tick-borne bacterial diseases]. | molecular tools have been used to detect rickettsiae in ticks. in ixodes ricinus ticks collected in france, we detected for the first time there an emerging pathogen, rickettsia helvetica, and an ehrlichia sp, closely related to the agent of human granulocytic ehrlichiosis. in guadeloupe (french west indies), we described the occurrence of african tick-bite fever due to rickettsia africae, which had been previously reported in sub-saharan africa only. in africa, we completed our knowledge about ... | 2001 | 11282521 |
| serologic evidence of infection with granulocytic ehrlichiae in black bears in pennsylvania. | serum samples from 381 black bears (ursus americanus) killed in pennsylvania (usa) on 24 november 1997 were analyzed for antibodies reactive to the agent of human granulocytic ehrlichiosis (hge; ehrlichia sp.) by indirect immunofluorescence assay. antibody reactivity to hge antigen was detected in 21% (81/381) of the samples collected. reactive samples were reported from 56% (14/25) of the counties where bear samples were collected. endpoint antibody titer ranged from 1:8 to 1:16, 192, with a ge ... | 2002 | 11838228 |
| [serologic evidence for human ehrlichiosis in chile]. | ehrlichiosis is a non contagious infectious disease, mainly transmitted by thick bites. in 1998, this infection was detected in dogs, for the first time, in chile. | 2003 | 12643221 |
| detection of ehrlichia spp., anaplasma spp., rickettsia spp., and other eubacteria in ticks from the thai-myanmar border and vietnam. | a total of 650 ticks, including 13 species from five genera, were collected from animals, from people, or by flagging of the vegetation at sites on the thai-myanmar border and in vietnam. they were tested by pcr to detect dna of bacteria of the order rickettsiales: three anaplasma spp. were detected in ticks collected in thailand, including (i) anaplasma sp. strain anda465, which was considered a genotype of anaplasma platys (formerly ehrlichia platys) and which was obtained from dermacentor aur ... | 2003 | 12682151 |
| coinfection with multiple tick-borne pathogens in a walker hound kennel in north carolina. | both dogs and humans can be coinfected with various ehrlichia, bartonella, rickettsia, and babesia species. we investigated a kennel of sick walker hounds and their owners in southeastern north carolina for evidence of tick-borne infections and associated risk factors. a high degree of coinfection was documented in the dog population. of the 27 dogs, 26 were seroreactive to an ehrlichia sp., 16 to babesia canis, and 25 to bartonella vinsonii, and 22 seroconverted to rickettsia rickettsii antigen ... | 1999 | 10405413 |
| genetic diversity of bacterial agents detected in ticks removed from asymptomatic patients in northeastern italy. | a total of 360 ticks were removed from 353 asymptomatic subjects in belluno province, italy and surrounding areas, from 1998 to 2001. ticks were identified as ixodes ricinus (357), ixodes hexagonus (1), rhipicephalus sanguineus (1), and ixodes ventalloi (1). tick dna was investigated by pcr and subsequent sequencing of amplified products to identity associated bacterial agents. primers targeting different genes of rickettsia (glta and ompa), borrelia (16s rdna, rpob), francisella (16s rdna), and ... | 2003 | 12860623 |
| the coyote, a potential host for babesia canis and ehrlichia sp. | | 1964 | 14215497 |
| evidence of babesia microti infection in multi-infected ixodes persulcatus ticks in russia. | to detect babesia-infected ixodes persulcatus shulze in a suburb of st. petersburg, russia, 738 adult ticks were studied using babesia specific primers and pcr techniques. the entire sample (more than 1,200 individuals) was screened for the presence of borrelia spp., ehrlichia spp. and tick-borne encephalitis virus (tbev). all 7 ticks infected with babesia microti, were also infected with other pathogens (all 7 among 417 infected ticks, zero amongst the remaining 321 naive ones (chi2 = 5.25, p<0 ... | 2003 | 14635819 |
| detection of ehrlichial dna in haemaphysalis ticks recovered from dogs in japan that is closely related to a novel ehrlichia sp. found in cattle ticks from tibet, thailand, and africa. | tick dna samples from dogs in japan were examined for ehrlichia infection by 16s rrna gene-based pcr and sequencing. three positive samples were detected from haemaphysalis ticks, and higher levels of similarity (98.46 to 99.06%) were found to recently detected ehrlichia spp. from cattle ticks in tibet, thailand, and africa. | 2004 | 15004117 |
| vectors of tick-borne diseases and epidemiological situation in latvia in 1993-2002. | during the period of 1993-2002 an increase and the remarkable changes in the incidence of tick-borne encephalitis (tbe) and lyme borreliosis (lb) as well as annual activities of vector species were noticed. the highest increase of tbe morbidity in latvia has been observed in 1994 and 1995, and less expressed also in 1998 which was followed by a significant decrease during subsequent years. whereas the highest peak of lb morbidity has been noticed in 1998 with only a minor decrease during subsequ ... | 2004 | 15146983 |
| pathogens and symbionts in ticks: prevalence of anaplasma phagocytophilum (ehrlichia sp.), wolbachia sp., rickettsia sp., and babesia sp. in southern germany. | tick-transmitted diseases like tick-borne encephalitis and lyme borreliosis have been well known in germany for decades. ongoing research now gives an additional focus to a broad range of other bacteria and parasites in ticks like anaplasma phagocytophilum, former ehrlichia sp., rickettsia sp. and babesia sp. knowledge about the prevalence of these infectious agents in ticks is an important prerequisite for risk assessment of human diseases. therefore nymphs and adult ixodes ricinus ticks were c ... | 2004 | 15146989 |
| [morphologic identification of ehrlichia sp. in the platelets of patients infected with the human immunodeficiency virus in venezuela]. | to determine the frequency of infection by bacteria of the genus ehrlichia in a population of patients infected with the human immunodeficiency virus (hiv). as is widely recognized, hiv infection is usually followed by the acquired immunodeficiency syndrome (aids) after a variable period during which the individual can develop different types of opportunistic infections whose severity depends on his/her immune status. human ehrlichiosis is a newly-recognized tick-borne infectious disease caused ... | 2004 | 15729984 |
| experimental inoculation of beagle dogs with ehrlichia species detected from ixodes ovatus. | three beagle dogs were inoculated with mice spleen/liver homogenate infected with ehrlichia species detected from ixodes ovatus (eio) and one dog was used as a control. all three infected dogs did not show clinical signs of disease except for mild pyrexia throughout the 41-day study period. splenomegaly was observed from day 7 post-inoculation (p.i.) in two of the dogs. hematological and biochemical abnormalities included mild thrombocytopenia, hypoproteinaemia, hypoalbuminaemia and increased c- ... | 2006 | 16309840 |
| diversity of 16s rrna genes of new ehrlichia strains isolated from horses with clinical signs of potomac horse fever. | ehrlichia risticii is the causative agent of potomac horse fever. variations among the major antigens of different local e. risticii strains have been detected previously. to further assess genetic variability in this species or species complex, the sequences of the 16s rrna genes of several isolates obtained from sick horses diagnosed as having potomac horse fever were determined. the sequences of six isolates obtained from ohio and three isolates obtained from kentucky were amplified by pcr. t ... | 1995 | 7537065 |
| novel genetic variants of anaplasma phagocytophilum, anaplasma bovis, anaplasma centrale, and a novel ehrlichia sp. in wild deer and ticks on two major islands in japan. | wild deer are one of the important natural reservoir hosts of several species of ehrlichia and anaplasma that cause human ehrlichiosis or anaplasmosis in the united states and europe. the primary aim of the present study was to determine whether and what species of ehrlichia and anaplasma naturally infect deer in japan. blood samples obtained from wild deer on two major japanese islands, hokkaido and honshu, were tested for the presence of ehrlichia and anaplasma by pcr assays and sequencing of ... | 2006 | 16461655 |
| detection of anaplasma phagocytophilum and ehrlichia sp. hf strains in ixodes ricinus ticks in brittany, france. | dna extracts from 156 tick pools, 18 blood specimens and 17 spleens from european woodmice (apodemus sylvaticus) collected in brittany, france were tested by pcr for the 16s rrna gene of anaplasmataceae. positive amplicons were sequenced and confirmed, either by amplification and sequencing of a second gene, or by a second pcr specific for the p44 and glta genes of anaplasma phagocytophilum and the glta gene of ehrlichia sp. hf. in addition to a. phagocytophilum, the study detected ehrlichia sp. ... | 2007 | 17391395 |
| isolation and establishment of the raccoon ehrlichia-like agent in tick cell culture. | feral animals are reservoirs of emerging human pathogens, as well as carriers of closely related wildlife diseases. the latter may interfere with epidemiologic studies by inducing cross-reactive antibodies, or by providing false positive signals in pcr based tests. we cultured a novel intracellular bacterium from the blood of two raccoons (procyon lotor): rac413 and rac414. rac413 had been experimentally inoculated with blood from a wild-caught raccoon, and provided the material for a blood pass ... | 2007 | 17867909 |
| outbreak of canine monocytic ehrlichiosis in saudi arabia. | canine monocytic ehrlichiosis (cme) is a widespread tickborne infection of canids caused by ehrlichia canis, a gram-negative obligatory intracellular bacteria belonging to the family anaplasmataceae. cme is reported to have worldwide distribution, but its presence in a region requires the presence of the vector, the brown dog tick rhipicephalus sanguineus. | 2007 | 18041697 |
| clinical and hematological signs associated with dogs naturally infected by hepatozoon sp. and with other hematozoa: a retrospective study in uberlândia, minas gerais, brazil. | an evaluation was made of the clinical and hematological aspects of 115 dogs infected naturally by hepatozoon sp. and treated at the hospital school of the faculty of veterinary medicine in uberlândia, mg, brazil. of the 115 dogs for whom peripheral blood films were evaluated, 89 (77.39%) presented parasitemia by hepatozoon sp. solely, while 26 (22.61%) had combination of hepatozoon sp., babesia sp. and ehrlichia sp. young male dogs less than a year old, of undefined breed (ub), were the most co ... | 2008 | 18304739 |
| two usa ehrlichia spp. cause febrile illness in goats. | ehrlichia spp. are not currently recognized as a cause of illness in goats in the usa, but three ehrlichia are enzootic in lone star ticks (amblyomma americanum) in the eastern usa, and related bacteria in other countries cause illness in goats. we exposed naïve goats to ehrlichia-infected amblyomma and demonstrated that infection and clinical illness can be caused by two usa species, e. ewingii and the recently discovered panola mountain ehrlichia sp. clinical features in all five goats are des ... | 2008 | 18328644 |
| geographic distribution and genetic diversity of the ehrlichia sp. from panola mountain in amblyomma americanum. | a novel ehrlichia, closely related to ehrlichia ruminantium, was recently discovered from panola mountain state park, ga, usa. we conducted a study to determine if this agent was recently introduced into the united states. | 2008 | 18433500 |
| natural and experimental infection of white-tailed deer (odocoileus virginianus) from the united states with an ehrlichia sp. closely related to ehrlichia ruminantium. | an ehrlichia sp. (panola mountain [pm] ehrlichia sp.) closely related to ehrlichia ruminantium was recently detected in a domestic goat experimentally infested with lone star ticks (lsts, amblyomma americanum) collected from georgia, usa. the infected goat exhibited pyrexia and mild clinical pathologic abnormalities consistent with ehrlichiosis. at least two other ehrlichia species (ehrlichia chaffeensis and ehrlichia ewingii) are maintained in nature by a cycle involving lsts as the primary vec ... | 2008 | 18436670 |
| adrenal gland hemorrhage in patients with fatal bacterial infections. | a wide spectrum of adrenal gland pathology is seen during bacterial infections. hemorrhage is particularly associated with meningococcemia, while abscesses have been described with several neonatal infections. we studied adrenal gland histopathology of 65 patients with bacterial infections documented in a variety of tissues by using immunohistochemistry. the infections diagnosed included neisseria meningitidies, group a streptococcus, rickettsia rickettsii, streptococcus pneumoniae, staphylococc ... | 2008 | 18500257 |
| clinical, serological, and molecular evidence of ehrlichiosis and anaplasmosis in dogs in tunisia. | a seroepidemiological survey was conducted in five bioclimatic areas of tunisia to determine the prevalence of antibodies to ehrlichia canis and anaplasma phagocytophilum antigens, surrogate markers of the agents of canine monocytic ehrlichiosis (cme) and canine granulocytic ehrlichiosis, respectively. among 286 collected sera, 54.2% and 25.2% were seropositive for e. canis and a. phagocytophilum, respectively, by the indirect immunofluorescence antibody (ifa) test. clinical and hematological te ... | 2009 | 19018569 |
| the application of pcr and reverse line blot hybridization to detect arthropod-borne hemopathogens of dogs and cats in trinidad. | arthropod-borne diseases are important causes of morbidity and mortality of companion animals in trinidad. as clinical signs are vague, more sensitive methods to diagnose these diseases based on the polymerase chain reaction (pcr) followed by reverse line blot hybridization (rlb) of amplified products are being developed. an rlb of 14 oligonucleotide probes coupled with polymerase chain-amplified regions of 16s rrna or 18s rrna genes of hemoparasites from cats and dogs detected ehrlichia canis, ... | 2008 | 19120209 |
| presence of a novel ehrlichia sp. in ixodes granulatus found in okinawa, japan. | ehrlichia-specific dna fragments of ehrlichia omp-1 and groel genes were found in two i. granulatus ticks which had been collected from wild small mammals in a subtropical zone in japan. the dna sequences of groel and 16srdna of the suspected ehrlichia were clustered into a group of e. chaffeensis, e. muris, and ehrlichia sp. hf565 found in i. ovatus, but were distinctly different. therefore the ehrlichia strain was designated as a novel ehrlichia sp. 360. the ehrlichia sp. 360 was detected in i ... | 2009 | 19291093 |
| diagnosis of ehrlichia ewingii infection by pcr in a puppy from ohio. | an 8-week-old, male, german shepherd-cross puppy found in southeastern ohio was presented to the ohio state university veterinary teaching hospital for evaluation of lameness and lethargy. fever and joint effusion involving multiple joints were identified on physical examination. results of a cbc included mild anemia, mature neutrophilia, monocytosis, and hyperglobulinemia. rickettsial morulae were identified within neutrophils in joint fluid and peripheral blood. both initial and convalescent s ... | 2009 | 19351337 |
| ticks and tick-borne pathogens and putative symbionts of black bears (ursus americanus floridanus) from georgia and florida. | ticks were collected from 38 black bears (ursus americanus floridanus) from northwestern florida (n = 18) from 2003 to 2005 and southern georgia (n = 20) in 2006. five species (amblyomma americanum, a. maculatum, dermacentor variabilis, ixodes scapularis, and i. affinis) were collected from florida bears, and 4 species (a. americanum, a. maculatum, d. variabilis, i. scapularis) were collected from bears in georgia. ixodes scapularis was the most frequently collected tick, followed by d. variabil ... | 2009 | 19413369 |
| molecular and serologic detection of ehrlichia spp. in endangered brazilian wild captive felids. | ehrlichiosis, an emergent tick-borne disease that affects both humans and animals, may represent a threat to the survival and preservation of wild felids in brazil. there are few studies of ehrlichiosis in wild felids in brazil, but ehrlichia spp. are present in domestic cats. antibodies to ehrlichia canis have been reported in a puma (puma concolor). in this study we assessed the presence of these hemoparasites in the blood of brazilian wild captive felids. of the 72 animals tested, 5 (7%) were ... | 2010 | 20688716 |
| tick-borne blood parasites in nyala (tragelaphus angasii, gray 1849) from kwazulu-natal, south africa. | a total of 97 blood samples of nyala (tragelaphus angasii, gray 1849) from south africa were tested for the presence of tick-borne haemoparasites by means of polymerase chain reaction (pcr) and reverse line blot (rlb) hybridization. the majority of blood samples contained several different haemoparasites, often in combination. prevalent haemoparasites were theileria sp. (kudu), t. buffeli, theileria sp. (sable), t. bicornis, ehrlichia sp. omatjenne, anaplasma marginale and a. bovis. this serves ... | 2010 | 21145660 |
| [an outbreak of endemic typhus in baoshan city, yunnan province.] | objective: to understand the epidemiologic characteristics of endemic typhus in baoshan city. methods: epidemiological data were collected and characteristics were analyzed. igg antibody (ab) of rickettsia mooseri and orientia tsutsugamushi in serum of patients were tested using both weil-felix and ifa method. the rickettsia mooseri glta gene, rickettsia prowazekii glta gene, orientia tsutsugamushi 56 kda protein gene, sfgr ompa gene, ehrlichia sp. 16s rrna gene and anaplasma sp. 16s rrna gene i ... | 2011 | 21518541 |
| serum acute phase proteins as clinical phase indicators and outcome predictors in naturally occurring canine monocytic ehrlichiosis. | background: canine monocytic ehrlichiosis (cme), caused by ehrlichia canis, is an important tick-borne disease of global importance. currently, limited information is available on the diagnostic and prognostic value of acute phase proteins (apps) in dogs naturally infected with e. canis. hypothesis: apps may be useful indicators of the clinical phase of cme and predictive of the clinical outcome (death or survival). animals: fifty-six dogs naturally infected with e. canis and 7 clinically health ... | 2011 | 21564293 |
| [in vitro pathogenicity of fungic formulation on nymphs and adults of rhipicephalus sanguineus (latreile, 1806) (acari:ixodidae)]. | rhipicephalus sanguineus is responsible for transmitting several pathogenic agents, such as: babesia sp. and ehrlichia sp.. this species is known as the brown dog tick and has wide geographical distribution. the purpose of this work was to evaluate the pathogenicity of biological formulations of beauveria bassiana and metarhizium anisopliae applied to the fed nymphs and adults of r. sanguineus under laboratory conditions. the following treatments were evaluated: control, distilled water with twe ... | 2005 | 16229753 |
| [enzootic reservoir for new ixodes ricinus-transmitted infections]. | in the last decade new tick-transmitted zoonoses have emerged as threats to the public health in europe. they are mostly due to infection by borrelia burgdorferi s.i., babesia divergens, b. microti and ehrlichia sp. all these pathogens are transmitted by the rodent-feeding ticks of ixodes persulcatus "complex". also there is first serological information of coexistence of antibodies to b. burgdorferi, b. microti and ehrlichia sp. in human exposured to these multiple tick-transmitted pathogens. a ... | 1999 | 16886454 |
| molecular survey of babesia microti, ehrlichia species and candidatus neoehrlichia mikurensis in wild rodents from shimane prefecture, japan. | a significant number of patients are diagnosed with "fevers of unknown origin" (fuo) in shimane prefecture in japan where tick-borne diseases are endemic. we conducted molecular surveys for babesia microti, ehrlichia species, and candidatus neoehrlichia mikurensis in 62 fuo cases and 62 wild rodents from shimane prefecture, japan. pcr using primers specific for the babesia 18s small-subunit rrna (rdna) gene and anaplasmataceae groesl amplified products from 45% (28/62) and 25.8% (16/62) of captu ... | 2007 | 17446675 |
| histopathologic, immunohistochemical, and polymerase chain reaction assays in the study of cases with fatal sporadic myocarditis. | paraffin tissue blocks from 27 cases with sporadic myocarditis were collected during a 12-year period at a single medical examiner's office. blocks were studied by using histopathology; immunohistochemistry for viruses (adenovirus, enterovirus, influenza a and b, and human herpes types 4 and 5), bacteria (neisseria meningitidis, ehrlichia sp, spotted fever group rickettsia) and parasites (toxoplasma gondii and trypanosoma cruzi); and polymerase chain reaction (pcr)/rt-pcr for adenovirus and ente ... | 2007 | 17602724 |
| tick-borne bacteria in free-living jaguars (panthera onca) in pantanal, brazil. | abstract tick-borne bacteria were investigated in 10 free-living jaguars and their ticks in the pantanal biome, brazil. jaguar sera were tested by indirect fluorescent antibody assays using rickettsia rickettsii, rickettsia parkeri, rickettsia amblyommii, rickettsia rhipicephali, rickettsia felis, rickettsia bellii, ehrlichia canis, and coxiella burnetii as crude antigens. all 10 jaguar sera reacted (titer ≥64) to at least one rickettsia species; 4 and 3 sera reacted with e. canis and c. burneti ... | 2011 | 21612532 |
| molecular detection and identification of anaplasma and ehrlichia species in cattle from turkey. | bovine anaplasmosis is a tick-borne rickettsial disease widespread in tropical and subtropical areas. we investigated the presence and distribution of anaplasma spp. in cattle from 6 provinces in turkey. for amplification of the segment spanning the v1 region of the 16s ribosomal rna (rrna) gene of anaplasma species, a reverse line blot (rlb) hybridization assay was performed on 389 blood samples. rlb identified anaplasma infections in 9.0% (35/389) of the samples. the most frequently found spec ... | 2011 | 21771539 |
| genetic diversity of anaplasma and ehrlichia in the asian part of russia. | totally, 2590 questing adult ixodes persulcatus ticks and 1458 small mammals from ural, siberia, and the far east as well as 53 haemaphysalis concinna, 136 haem. japonica, and 43 dermacentor silvarum ticks--exclusively adults--from the far east were examined for the presence of ehrlichia and anaplasma by nested pcr based on the 16s rrna gene. both anaplasma phagocytophilum and ehrlichia muris were found in i. persulcatus and small mammals from all the studied regions. myodes spp., microtus spp., ... | 2010 | 21771512 |
| Molecular Study of Free-ranging Mule Deer and White-tailed Deer from British Columbia, Canada, for Evidence of Anaplasma spp. and Ehrlichia spp. | Twenty-three free-ranging white-tailed deer (WTD; Odocoileus virginianus) and six mule deer (MD; Odocoileus hemionus) from south-central British Columbia, Canada, were tested for Anaplasma marginale by msp5 gene-specific PCR and Ehrlichia spp. by 16S rRNA or citrate synthase (gltA) gene-specific PCR, as well as by PCR with universal 16S rRNA primers detecting a wide range of bacteria. No deer tested positive for A. marginale. Amplification with universal 16S rRNA primers followed by sequencing o ... | 2011 | 21933360 |
| serological cross-reactivity between anaplasma marginale and an ehrlichia species in naturally and experimentally infected cattle. | seroconversion and cross-reactivity in cattle infected with anaplasma marginale or a recently described ehrlichia species (bov2010 from british columbia, canada) were investigated. the study used 76 samples from 20 animals, a commercially available competitive enzyme-linked immunosorbent assay (celisa) for bovine anaplasmosis, and an indirect fluorescent antibody test (ifat). blood smear examination and/or polymerase chain reaction assay were performed to confirm or rule out the presence of anap ... | 2011 | 22362799 |