| characterization of vibrio anguillarum and closely related species isolated from farmed fish in norway. | a total of 264 bacterial strains tentatively or definitely classified as vibrio anguillarum were examined. the strains were isolated from diseased or healthy norwegian fish after routine autopsy. with the exception of five isolates from wild saithe (pollachius virens), the strains originated from nine different species of farmed fish. the bacteria were subjected to morphological, physiological, and biochemical studies, numerical taxonomical analyses, serotyping by slide agglutination and enzyme- ... | 1991 | 1768147 |
| vibrios associated with red tides caused by mesodinium rubrum. | vibrios were isolated from red tides caused by mesodinium rubrum and also throughout the year in the ria de pontevedra, spain. the isolates were grouped into 14 phena by numerical toxonomy. strains associated with red tides were restricted to four phena: phena i and ii were vibrio alginolyticus, and phena iii and iv were vibrio tubiashii and vibrio anguillarum, respectively. v. anguillarum-like strains (phena v through xi) predominated throughout the year outside the red tide areas. cytotoxicity ... | 1990 | 2268167 |
| inhibitory effects of ovoglobulins on bacillary necrosis in larvae of the pacific oyster, crassostrea gigas. | in order to develop an alternative method to antibiotics for preventing bacillary necrosis in bivalve mollusc larvae, we examined the effects of ovoglobulins (proteins derived from the whites of hens' eggs) on the survival of larvae of the pacific oyster crassostrea gigas. the pathogenic vibrio tubiashii (atcc 19106) was used to infect larvae of the pacific oyster. v. tubiashii showed strong pathogenicity to oyster larvae, causing 100% mortality after experimental exposure for 24 h at a concentr ... | 2000 | 10753597 |
| purification and characterization of a vulnificolysin-like cytolysin produced by vibrio tubiashii. | an extracellular cytolysin from vibrio tubiashii was purified by sequential hydrophobic interaction chromatography with phenyl-sepharose cl-4b and gel filtration with sephacryl s-200. this protein is sensitive to heat and proteases, is inhibited by cholesterol, and has a molecular weight of 59,000 and an isoelectric point of 5.3. in addition to lysing various erythrocytes, it is cytolytic and/or cytotoxic to chinese hamster ovary cells, caco-2 cells, and atlantic menhaden liver cells in tissue c ... | 2001 | 11472951 |
| vibrios isolated from the cultured manila clam (ruditapes philippinarum): numerical taxonomy and antibacterial activities. | a numerical taxonomic study of halophilic vibrio isolated from healthy and brown ring disease (brd) affected manila clams (ruditapes philippinarum), harvested from the atlantic coast of south-western spain, was performed. | 2002 | 12174042 |
| vibrio neptunius sp. nov., vibrio brasiliensis sp. nov. and vibrio xuii sp. nov., isolated from the marine aquaculture environment (bivalves, fish, rotifers and shrimps). | the fluorescent amplified fragment length polymorphism (faflp) groups a5 (21 isolates), a8 (6 isolates) and a23 (3 isolates) distinguished in an earlier paper (thompson et al., syst appl microbiol 24, 520-538, 2001) were examined in more depth. these three groups were phylogenetically related to vibrio tubiashii, but dna-dna hybridization experiments proved that the three aflp groups are in fact novel species. chemotaxonomic and phenotypic analyses further revealed several differences among the ... | 2003 | 12656180 |
| vibrio coralliilyticus sp. nov., a temperature-dependent pathogen of the coral pocillopora damicornis. | vibrio sp. yb1t (=atcc baa-450t =lmg 20984t), the aetiological agent of tissue lysis of the coral pocillopora damicornis, was characterized as a novel vibrio species on the basis of 16s rdna sequence, dna-dna hybridization data (g + c content is 45.6 mol%), aflp and gtg5-pcr genomic fingerprinting patterns and phenotypic properties, including the cellular fatty acid profile. the predominant fatty acids were 16:0 and 18:1 omega7c. the name vibrio coralliilyticus sp. nov. is proposed for the novel ... | 2003 | 12656189 |
| isolation and characterization of a zinc-containing metalloprotease expressed by vibrio tubiashii. | a vibrio tubiashii hemagglutinin, a protease, was purified by ammonium sulfate precipitation, gel filtration, and hydrophobic interaction chromatography. it agglutinates sheep, chicken, bovine, rabbit, guinea pig, and human erythrocytes. it has a molecular mass of 35 kda, isoelectric points of 3.5 and 3.7, and is inhibited by ortho-phenanthro line, phosphoramidon, and zincov. the n-terminal amino acid sequence (ala-gln-ala-thr-gly-thr-gly- pro-gly-gly-asn-gln-lys-thr-gly-gln- tyr-asn-phe-gly) ha ... | 2003 | 14608388 |
| use of reca as an alternative phylogenetic marker in the family vibrionaceae. | this study analysed the usefulness of reca gene sequences as an alternative phylogenetic and/or identification marker for vibrios. the reca sequences suggest that the genus vibrio is polyphyletic. the high heterogeneity observed within vibrios was congruent with former polyphasic taxonomic studies on this group. photobacterium species clustered together and apparently nested within vibrios, while grimontia hollisae was apart from other vibrios. within the vibrios, vibrio cholerae and vibrio mimi ... | 2004 | 15143042 |
| interactive effects of temperature and copper on immunocompetence and disease susceptibility in mussels (mytilus edulis). | the interactive effects of temperature and copper on immune function and consequently disease susceptibility of the marine mussel, mytilus edulis were investigated. two studies were carried out, the first involved sequential exposure to copper at 0.02 and 0.05 ppm followed by the bacterium vibrio tubiashii. in the second study, mussels were simultaneously exposed to copper and v. tubiashii. both studies were carried out at 10 and 15 degrees c, to ascertain whether temperature had an additional e ... | 2004 | 15312716 |
| functional characterization of two type iii secretion systems of vibrio parahaemolyticus. | vibrio parahaemolyticus, a gram-negative marine bacterium, is a worldwide cause of food-borne gastroenteritis. recent genome sequencing of the clinical v. parahaemolyticus strain rimd2210633 identified two sets of genes for the type iii secretion system (ttss), ttss1 and ttss2. here, we constructed a series of mutant strains from rimd2210633 to determine whether the two putative ttss apparatus are functional. the cytotoxic activity of mutant strains having a deletion in one of the ttss1 genes wa ... | 2004 | 15501799 |
| the extracellular metalloprotease of vibrio tubiashii is a major virulence factor for pacific oyster (crassostrea gigas) larvae. | vibrio tubiashii is a recently reemerging pathogen of larval bivalve mollusks, causing both toxigenic and invasive disease. marine vibrio spp. produce an array of extracellular products as potential pathogenicity factors. culture supernatants of v. tubiashii have been shown to be toxic to oyster larvae and were reported to contain a metalloprotease and a cytolysin/hemolysin. however, the structural genes responsible for these proteins have yet to be identified, and it is uncertain which extracel ... | 2008 | 18456850 |
| vibrio porteresiae sp. nov., a diazotrophic bacterium isolated from a mangrove-associated wild rice (porteresia coarctata tateoka). | two facultatively anaerobic, nitrogen-fixing bacteria (strains mssrf30(t) and mssrf31) were isolated from a mangrove-associated wild rice (porteresia coarctata tateoka). these strains were determined to be nitrogen-fixers using the acetylene reduction assay and by pcr detection of a nifh gene amplicon. phylogenetic analyses based on 16s rrna gene sequences indicated that the novel strains were most closely related to vibrio fluvialis lmg 7894(t) (96.8 % gene sequence similarity), vibrio furnissi ... | 2008 | 18599703 |
| a novel peptidoglycan recognition protein containing a goose-type lysozyme domain from the pacific oyster, crassostrea gigas. | peptidoglycan recognition protein (pgrp) is considered an essential molecule for effective immunity in invertebrates by its detection and clarification of invading bacteria. bivalve mollusks also possess pgrp systems for self-defense, however, their functions in bivalves remain to be understood. in the present study, cdna of a novel pgrp was identified from the pacific oyster, crassostrea gigas, using est-based race pcr. this novel pgrp is homologous to short pgrps and the presence of a signal p ... | 2009 | 19246096 |
| the extracellular metalloprotease of vibrio tubiashii directly inhibits its extracellular haemolysin. | vibrio tubiashii is a re-emerging pathogen of molluscs that secretes a variety of extracellular products (ecps), including a metalloprotease and a cytolysin/haemolysin. previously, we reported that the v. tubiashii haemolysin locus consists of two orfs (vthb and vtha), similar to that of the homologous haemolysin genes (vvhb and vvha) found in vibrio vulnificus. here, we demonstrate that the concomitant expression of both v. tubiashii genes resulted in significantly higher haemolytic activity th ... | 2009 | 19389767 |
| transcriptome response of the pacific oyster (crassostrea gigas) to infection with vibrio tubiashii using cdna aflp differential display. | we used qualitative complementary dna-amplified fragment length polymorphism (cdna-aflp) differential display analysis and real-time, quantitative pcr (rt-qpcr) to identify genes in the pacific oyster crassostrea gigas, whose transcription either changes in response to exposure to a pathogenic bacterium (vibrio tubiashii) or varies between families known to differ in sensitivity to heat stress, before and at 12 and 36 h after bacterial exposure at a temperature of 25 degrees c. these conditions ... | 2009 | 19456319 |
| virulence of metalloproteases produced by vibrio species on pacific oyster crassostrea gigas larvae. | vibrio tubiashii, a pathogen of shellfish larvae and juveniles, produces several extracellular products. here, we document that culture supernatants of several marine vibrio species showed toxicity to oyster larvae. treatment of these supernatants with edta not only severely diminished proteolytic activities, but also dramatically reduced toxicity to the larvae. culture supernatants of metalloprotease-deficient mutants of v. tubiashii, v. cholerae, and v. splendidus were impaired in their abilit ... | 2009 | 19694172 |
| tetr-type transcriptional regulator vtpr functions as a global regulator in vibrio tubiashii. | vibrio tubiashii, a causative agent of severe shellfish larval disease, produces multiple extracellular proteins, including a metalloprotease (vtpa), as potential virulence factors. we previously reported that vtpa is toxic for pacific oyster (crassostrea gigas) larvae. in this study, we show that extracellular protease production by v. tubiashii was much reduced by elevated salt concentrations, as well as by elevated temperatures. in addition, v. tubiashii produced dramatically less protease in ... | 2009 | 19837838 |
| permanent draft genome sequence of vibrio tubiashii strain ncimb 1337 (atcc19106). | vibrio tubiashii ncimb 1337 is a major and increasingly prevalent pathogen of bivalve mollusks, and shares a close phylogenetic relationship with both v. orientalis and v. coralliilyticus. it is a gram-negative, curved rod-shaped bacterium, originally isolated from a moribund juvenile oyster, and is both oxidase and catalase positive. it is capable of growth under both aerobic and anaerobic conditions. here we describe the features of this organism, together with the draft genome and annotation. ... | 2011 | 21677855 |
| influence of iron-chelated growth conditions on outer membrane protein production and virulence of vibrio tubiashii. | growth of two vibrio tubiashii strains under iron-chelated conditions resulted in the production of a hydroxymate-like siderophore, and expression of outer membrane proteins with homologies to proteins in vibrio cholerae and vibrio vulnificus which were not seen in cells grown under non-chelated growth conditions. pcr analysis using primers based on listonella anguillarum's ferric uptake repressor protein (fur) gene detected a 316-ábp fur gene homolog which also had sequence homology to the fur ... | 2011 | 21839393 |
| cloning and partial characterization of a novel hemolysin gene of vibrio tubiashii and the development of a pcr-based detection assay. | vibrio tubiashii expresses virulence factors, such as a vulnificolysin-like hemolysin or cytolysin and a zinc metalloprotease, similar to those of other pathogenic vibrios. in this study, we report the cloning of a novel hemolysin gene of v. tubiashii in escherichia coli . a v. tubiashii gene library was screened for hemolytic activity on sheep blood agar. three hemolytic clones pgem:hly1, pgem:hly2, and pgem:hly3 were sequenced, and the sequences showed a strong homology to the riba gene coding ... | 2011 | 21854089 |
| vibrio caribbeanicus sp. nov., isolated from marine sponge scleritoderma cyanea. | a gram-negative, oxidase-positive, catalase-negative, facultatively anaerobic, motile and curved rod-shaped bacterium was isolated from a marine sponge (phylum porifera, scleritoderma cyanea) collected from a depth of 795 feet off of the west coast of curacao. on the basis of 16s rrna gene sequencing, strain n384(t) was shown to belong to the genus vibrio, most closely related to v. brasiliensis lmg 20546(t) (98.8%), v. nigripulchritudo atcc 27043(t) (98.5%), v. tubiashii atcc 19109(t) (98.6%) a ... | 2011 | 21930677 |
| identification of potential general markers of disease resistance in american oysters, crassostrea virginica through gene expression studies. | several diseases have a significant impact on american oyster populations in the atlantic coasts of north america. knowledge about the responses of oysters to pathogenic challenge could help in identifying potential markers of disease resistance and biomarkers of the health status of an oyster population. a previous analysis of the transcriptome of resistant and susceptible american oysters in response to challenge with the bacterial pathogen roseovarius crassostreae, as well as sequencing of su ... | 2014 | 24973516 |
| vibrio bivalvicida sp. nov., a novel larval pathogen for bivalve molluscs reared in a hatchery. | three isolates were obtained from cultures of carpet shell clam (ruditapes decussatus) reared in a bivalve hatchery (galicia, nw spain) from different sources: healthy broodstock, moribund larvae and the seawater corresponding to the larval tank. all isolates were studied by a polyphasic approach, including a phylogenetic analysis based on concatenated sequences of the five housekeeping genes ftsz, gyrb, pyrh, reca and rpoa. the analysis supported their inclusion in the orientalis clade of the g ... | 2016 | 26654527 |
| reclassification of the larval pathogen for marine bivalves vibrio tubiashii subsp. europaeus as vibrio europaeus sp. nov. | the orientalis clade has a relevant significance for bivalve aquaculture since it includes the pathogens vibrio bivalvicida, vibrio tubiashii subsp. tubiashii and vibrio tubiashii subsp. europaeus. however, the previous taxonomic description of the subspecies of v. tubiashii shows some incongruities that should be emended. in the genomic age, the comparison between genome assemblies is the key to clarify the taxonomic position of both subspecies. with this purpose, we have tested the ability of ... | 2016 | 27538951 |
| draft genome sequence of the emerging bivalve pathogen vibrio tubiashii subsp. europaeus. | vibrio tubiashii subsp. europaeus is a bivalve pathogen isolated during episodes of mortality affecting larval cultures in different shellfish hatcheries. here, we announce the draft genome sequence of the type strain pp-638 and describe potential virulence factors, which may provide insight into the mechanism of pathogenicity. | 2016 | 27469949 |
| identification of a bacterial pathogen associated with porites white patch syndrome in the western indian ocean. | porites white patch syndrome (pwps) is a coral disease recently described in the western indian ocean. this study aimed to isolate and identify potential pathogens associated with pwps utilizing both culture and nonculture screening techniques and inoculation trials. a total of 14 bacterial strains (those dominant in disease lesions, absent or rare in healthy tissues and considered potential pathogens in a previous study) were cultured and used to experimentally inoculate otherwise healthy indiv ... | 2015 | 26193772 |
| re-emergence of vibrio tubiashii in bivalve shellfish aquaculture: severity, environmental drivers, geographic extent and management. | during 2006 and 2007, we documented the re-emergence of severe episodes of vibriosis caused by vibrio tubiashii in shellfish hatcheries on the west coast of north america. lost larval and juvenile production included 3 previously undescribed hosts, pacific (crassostrea gigas) and kumamoto (c. sikamea) oysters and geoduck clams panope abrupta, with a 2007 decline in larval oyster production of approximately 59% in one hatchery. losses of larval and juvenile bivalves were linked to v. tubiashii bl ... | 2008 | 19149375 |
| development of a quantitative real-time pcr assay for detection of vibrio tubiashii targeting the metalloprotease gene. | vibrio tubiashii has recently re-emerged as a pathogen of bivalve larvae, causing a marked increase in the mortality of these species within shellfish rearing facilities. this has resulted in substantial losses of seed production and thus created the need for specific as well as sensitive detection methods for this pathogen. in this project, quantitative pcr (qpcr) primers were developed and optimized based upon analysis of the v. tubiashii vtpa gene sequence, encoding a metalloprotease known to ... | 2009 | 19135097 |
| purification and antibacterial characterization of a novel isoform of the manila clam lectin (mcl-4) from the plasma of the manila clam, ruditapes philippinarum. | in many bivalve molluscs, lectins are present in the hemolymph and are thought to be important for internal host defense mechanisms. for this study, we purified a novel isoform of the manila clam lectin (designated mcl-4) from the plasma of the manila clam, ruditapes philippinarum, using affinity chromatography and gel filtration. native page results showed that the mcl-4 consisted of 70 kda protein. mcl-4 was found to be composed of 58-kda and 43-kda bands when examined using sds-page under red ... | 2008 | 18337136 |
| isolation and characterization of vibrio tubiashii outer membrane proteins and determination of a toxr homolog. | outer membrane proteins (omps) expressed by vibrio tubiashii under different environmental growth conditions were characterized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, n-terminal amino acid sequencing, and pcr analyses. results showed the presence of a 38- to 40-kda ompu-like protein and ompu gene, a maltoporin-like protein, several novel omps, and a regulatory toxr homolog. | 2008 | 18083865 |
| identification and tissue expression analysis of c-type lectin and galectin in the pacific oyster, crassostrea gigas. | as an initial step in the functional analysis of lectins in the pacific oyster, crassostrea gigas, we attempted to obtain the full coding sequences of c. gigas lectins and conduct tissue expression analyses. to obtain lectin genes quickly, we identified c. gigas expressed sequence tags that coded for lectins in genbank, and selected three encoding partial sequences of c-type lectin 1 (cgclec-1), galectin (cggal) and fucolectin. we obtained full open reading frames of cgclec-1 and cggal cdnas by ... | 2008 | 17913530 |
| nucleotide sequence of the 5s rrna from listonella (vibrio) ordalii atcc 33509 and listonella (vibrio) tubiashii atcc 19105. | | 1988 | 3368324 |
| complement-like activity in the sea star, asterias forbesi. | the coelomic fluid (cf) of asterias forbesi was examined for the presence of complement-like activity. cell-free cf contains hemolytic activity for unsensitized rabbit erythrocytes (e) but not unsensitized or antibody-coated sheep e. target e sensitivity is not dependent upon phylogenetic proximity of erythrocyte species to asterias. the activity is optimally assayed at 25 degrees c, has a sigmoidal dose-response curve, and is heat-sensitive (56 degrees c). the activity is divalent cation depend ... | 1990 | 2338154 |
| genes similar to the vibrio parahaemolyticus virulence-related genes tdh, tlh, and vscc2 occur in other vibrionaceae species isolated from a pristine estuary. | detection of the human pathogen vibrio parahaemolyticus often relies on molecular biological analysis of species-specific virulence factor genes. these genes have been employed in determinations of v. parahaemolyticus population numbers and the prevalence of pathogenic v. parahaemolyticus strains. strains of the vibrionaceae species photobacterium damselae, vibrio diabolicus, vibrio harveyi, and vibrio natriegens, as well as strains similar to vibrio tubiashii, were isolated from a pristine salt ... | 2014 | 24212573 |
| pathobiomes differ between two diseases affecting reef building coralline algae. | crustose coralline algae (cca) are major benthic calcifiers that play crucial roles in coral reef ecosystems. two diseases affecting cca have recently been investigated: coralline white band syndrome (cwbs) and coralline white patch disease (cwpd). these diseases can trigger major losses in cca cover on tropical coral reefs, but their causative agents remain unknown. here, we provide data from the first investigation of the bacterial communities associated with healthy and diseased cca tissues. ... | 2017 | 28919890 |
| development of monoclonal antibody-based assays for the detection of vibrio tubiashii zinc-metalloprotease (vtpa). | vibrio tubiashii has been linked to disease outbreaks in molluscan species, including oysters, geoducks, and clams. in particular, oyster hatcheries in the pacific northwest have been plagued by intermittent vibriosis since 2006. accurate detection of vibrios, including v. tubiashii, is critical to the hatcheries in order to allow for rapid remediation efforts. the current methods for detection of vibrio spp. are not ideal for use at the hatchery. plating samples require time and is not sensitiv ... | 2013 | 23685397 |
| ocean acidification and host-pathogen interactions: blue mussels, mytilus edulis, encountering vibrio tubiashii. | ocean acidification (oa) can shift the ecological balance between interacting organisms. in this study, we have used a model system to illustrate the interaction between a calcifying host organism, the blue mussel mytilus edulis and a common bivalve bacterial pathogen, vibrio tubiashii, with organisms being exposed to a level of acidification projected to occur by the end of the 21st century. oa exposures of the mussels were carried out in relative long-term (4 months) and short-term (4 days) ex ... | 2014 | 24147969 |
| first description of french v. tubiashii strains pathogenic to mollusk: i. characterization of isolates and detection during mortality events. | nine dominant bacterial isolates were obtained from different batches of crassostrea gigas spat experiencing high mortality rates in a french experimental hatchery/nursery in 2007. using phenotypic analysis combined with multilocus sequence analysis, the isolates were shown to be genetically close to the vibrio tubiashii type strain. based on (1) analyses of the reca gene sequences; (2) the results of dna-dna hybridization assays between 07/118 t2 (lmg 27884=cect 8426), which is a representative ... | 2014 | 24815818 |
| first description of french v. tubiashii strains pathogenic to mollusk: ii. characterization of properties of the proteolytic fraction of extracellular products. | extracellular products (ecps) of the french vibrio tubiashii strain 07/118 t2 were previously reported to be toxic for the pacific oyster crassostrea gigas. in this study we now assessed host cellular immune responses and bacterial potential effectors by which these ecps can be associated with host damages. the adhesion capacity (28% inhibition) and phagocytosis ability (56% inhibition) of oyster hemocytes were the main functions affected following in vitro contact between hemocytes and v. tubia ... | 2014 | 25252078 |
| complete genome sequence of the larval shellfish pathogen vibrio tubiashii type strain atcc 19109. | vibrio tubiashii is a larval shellfish pathogen. here, we report the first closed genome sequence for this species (atcc type strain 19109), which consists of two chromosomes (3,294,490 and 1,766,582 bp), two megaplasmids (251,408 and 122,808 bp), and two plasmids (57,076 and 47,973 bp). | 2014 | 25523763 |
| characterization of pathogenic vibrios isolated from bivalve hatcheries in galicia, nw atlantic coast of spain. description of vibrio tubiashii subsp. europaeus [corrected] subsp. nov. | the taxonomic position of the bivalve pathogen pp-638 was studied together with five similar isolates. the strains were isolated from flat oyster (ostrea edulis) and manila clam (venerupis philippinarum) cultures during outbreaks of disease in two shellfish hatcheries (galicia, nw spain). the pathogenicity, previously established for pp-638, was demonstrated with all isolates and for several bivalve species, including the original hosts. on the basis of phenotypic characterization and 16s rrna g ... | 2015 | 25555343 |
| whole-genome sequence of quorum-sensing vibrio tubiashii strain t33. | vibrio tubiashii strain t33 was isolated from the coastal waters of morib, malaysia, and was shown to possess quorum-sensing activity similar to that of its famous relative vibrio fischeri. here, the assembly and annotation of its genome are presented. | 2015 | 25555738 |
| factors other than metalloprotease are required for full virulence of french vibrio tubiashii isolates in oyster larvae. | vibrio tubiashii is a marine pathogen isolated from larval and juvenile bivalve molluscs that causes bacillary necrosis. recent studies demonstrated the isolation of this species in a french experimental hatchery/nursery affecting crassostrea gigas spat in 2007. here, using larvae of c. gigas as an interaction model, we showed that the french v. tubiashii is virulent to larvae and can cause bacillary necrosis symptoms with an ld50 of about 2.3 × 10(3) c.f.u. ml(-1) after 24 h. moreover, complete ... | 2015 | 25701736 |
| first report of vibrio tubiashii associated with a massive larval mortality event in a commercial hatchery of scallop argopecten purpuratus in chile. | the vpap30 strain was isolated as the highly predominant bacteria from an episode of massive larval mortality occurring in a commercial culture of the chilean scallop argopecten purpuratus. the main aims of this study were, to characterize and identify the pathogenic strain using biochemical and molecular methods to demonstrate its pathogenic activity on scallop larvae, to characterize its pathogenic properties and to describe the chronology of this pathology. the pathogenic strain was identifie ... | 2016 | 27703450 |
| mortality event involving larvae of the carpet shell clam ruditapes decussatus in a hatchery: isolation of the pathogen vibrio tubiashii subsp. europaeus. | diseases caused by bacteria belonging to the genus vibrio are a common, as yet unresolved, cause of mortality in shellfish hatcheries. in this study, we report the results of routine microbiological monitoring of larval cultures of the carpet shell clam ruditapes decussatus in a hatchery in galicia (nw spain). previous episodes of mortality with signs similar to those of vibriosis affecting other species in the installation indicated the possibility of bacterial infection and led to division of ... | 2017 | 28093770 |
| autolysis in vibrio tubiashii and vibrio coralliilyticus. | vibrio tubiashii has been linked to disease outbreaks in molluscan species, including oysters, geoducks, and clams, and shellfish hatcheries in the pacific northwest have been plagued by intermittent vibriosis outbreaks since 2006. like v. tubiashii, vibrio coralliilyticus has recently been described as an oyster pathogen in addition to its role in coral disease. here, we describe an autolysis phenotype in v. tubiashii and its close relative v. coralliilyticus and characterize the effects of env ... | 2014 | 24498982 |
| mortalities of eastern and pacific oyster larvae caused by the pathogens vibrio coralliilyticus and vibrio tubiashii. | vibrio tubiashii is reported to be a bacterial pathogen of larval eastern oysters (crassostrea virginica) and pacific oysters (crassostrea gigas) and has been associated with major hatchery crashes, causing shortages in seed oysters for commercial shellfish producers. another bacterium, vibrio coralliilyticus, a well-known coral pathogen, has recently been shown to elicit mortality in fish and shellfish. several strains of v. coralliilyticus, such as atcc 19105 and pacific isolates re22 and re98 ... | 2015 | 25344234 |
| complete genome sequence for the shellfish pathogen vibrio coralliilyticus re98 isolated from a shellfish hatchery. | vibrio coralliilyticus is a pathogen of corals and larval shellfish. publications on strain re98 list it as a vibrio tubiashii; however, whole genome sequencing confirms re98 as v. coralliilyticus containing a total of 6,037,824 bp consisting of two chromosomes (3,420,228 and 1,917,482 bp) and two megaplasmids (380,714 and 319,400 bp). | 2014 | 25523764 |
| contributions of tropodithietic acid and biofilm formation to the probiotic activity of phaeobacter inhibens. | the probiotic bacterium phaeobacter inhibens strain s4sm, isolated from the inner shell surface of a healthy oyster, secretes the antibiotic tropodithietic acid (tda), is an excellent biofilm former, and increases oyster larvae survival when challenged with bacterial pathogens. in this study, we investigated the specific roles of tda secretion and biofilm formation in the probiotic activity of s4sm. | 2016 | 26728027 |