| variations in rrna content of marine vibrio spp. during starvation-survival and recovery. | the degree and temporal context of variations in ribosome content during nutrient starvation of two copiotrophic marine bacteria, vibrio alginolyticus and vibrio furnissii, have been examined. the organisms were starved either by nutritional shift-down or by consumption of limiting nutrients resulting from growth into stationary phase. measurements of the amount of hybridization to 16s rrna-specific probes revealed that the cells retained between 10 and 26% of their original rrna content after 1 ... | 1992 | 1371659 |
| chitin utilization by marine bacteria. a physiological function for bacterial adhesion to immobilized carbohydrates. | chitin turnover is essential for recycling carbon and nitrogen in marine ecosystems. a key step in this process is the adhesion of marine bacteria to chitin-containing particulates. vibrio species were therefore surveyed for their ability to bind to immobilized carbohydrates, and one, vibrio furnissii, adhered to glycosides of three sugars, n-acetylglucosamine (the preferred ligand), d-mannose, and d-glucose. a single ca(2+)-requiring lectin is responsible for binding to the three sugars. cells ... | 1991 | 1761531 |
| chitin utilization by marine bacteria. degradation and catabolism of chitin oligosaccharides by vibrio furnissii. | chemotaxis of the marine bacterium vibrio furnissii to chitin oligosaccharides has been described (bassler, b. l., gibbons, p. j., yu, c., and roseman, s. (1991) j. biol. chem. 266, 24268-24275). some steps in catabolism of the oligosaccharides are reported here. glcnac, (glcnac)2, and (glcnac)3 are very rapidly consumed by intact cells, about 320 nmol of glcnac equivalents/min/mg of protein. (glcnac)4 is utilized somewhat more slowly. during these processes, there is virtually no release of hyd ... | 1991 | 1761533 |
| chemotaxis to chitin oligosaccharides by vibrio furnissii, a chitinivorous marine bacterium. | we have reported that vibrio furnissii, a chitinivorous marine bacterium, expresses a complex apparatus for adhesion/deadhesion to chitin analogues (1). in the present studies, we show that this organism exhibits a chemotactic response (swarming) to chitin oligosaccharides at concentrations as low as 10 microm. in contrast, v. furnissii exhibits slight to no chemotaxis to other utilizable compounds (glycerol, lactate, amino acids), with the exception of l-glutamic acid. v. furnissii may lack the ... | 1989 | 2742582 |
| enterotoxigenic substance and other toxins produced by vibrio fluvialis and vibrio furnissii. | | 1988 | 3172595 |
| vibrio furnissii isolated from intestinal contents of piglets. | | 1986 | 3606374 |
| the sugar-specific adhesion/deadhesion apparatus of the marine bacterium vibrio furnissii is a sensorium that continuously monitors nutrient levels in the environment. | our earlier studies on cell adhesion to immobilized carbohydrates are extended here to a marine bacterium, vibrio furnissii. apparently one lectin mediates the binding of these cells to glycosides of n-acetylglucosamine, mannose, and glucose covalently linked to agarose beads. kinetic studies show that protein synthesis is required for initiating and for maintaining adhesion to the glycosides. furthermore, a pro- mutant binds to glcnac-beads at pro concentrations insufficient to support cell gro ... | 1987 | 3689420 |
| vibrio furnissii (formerly aerogenic biogroup of vibrio fluvialis), a new species isolated from human feces and the environment. | strains formerly classified as the aerogenic (gas-producing) biogroup of vibrio fluvialis were shown by dna relatedness to be a separate species. the species was named vibrio furnissii sp. nov. (type strain atcc 35016 = cdc b3215). three strains of v. furnissii were 79% or more related to the type strain of v. furnissii and about 50% related to the type strain of v. fluvialis. v. fluvialis strains were 40 to 64% related to the type strain of v. furnissii. divergence in related sequences was only ... | 1983 | 6630464 |
| vibrio fluvialis and vibrio furnissii isolated from a stool sample of one patient. | vibrio fluvialis and vibrio furnissii have been associated with diarrhea but have rarely been isolated in the united states. we received strains of v. fluvialis and v. furnissii that were isolated from the stool of a 1-month-old baby. a description of these two strains and the case history of the patient are given in this report. | 1984 | 6746884 |
| identification of genes in a kg- phenotype of lactococcus garvieae, a fish pathogenic bacterium, whose proteins react with antikg- rabbit serum. | five different clones (sa1b05, sa1b10, sa2f01, sa8a11 and sa9h10) were isolated from the gene library of the lactococcus garvieae sa8201 (kg-) strain by immunological screening using rabbit serum against l. garvieae (kg-) phenotype cells. a western blot analysis indicated that the molecular sizes of immunologically detected proteins of sa1b05, sa1b10, sa2f01, sa8a11 and sa9h10, which were fused with lacz protein, were 25, 30, 28, 26 and 13 kda, respectively. the amino acid sequences of the immun ... | 1999 | 10588913 |
| isolation of vibrio and pseudomonas from brown shrimp (penaeus californiensis holmes) intestine. | bacteria of the genera vibrio, pseudomonas and aeromonas were isolated from the intestine of apparently healthy brown shrimp (penaeus californiensis holmes) cultured in a tidal pond. species from these genera of bacteria have been reported as shrimp pathogens and have been involved in human gastrointestinal disorders related to seafood consumption. isolation was done first in marine broth, then in selective media (tcbs, cetrimide and macconkey). the oxidase negative strains were discarded as ins ... | 1997 | 10932719 |
| isolation and characterization of a bacterium that produces hydrocarbons extracellularly which are equivalent to light oil. | a halotorelant bacterial strain that produces a significant amount of lipids from short-chain fatty acids was isolated from the sludge of a sewage disposal plant. this strain displayed a significant extracellular accumulation of lipids. the yield of lipids including hydrocarbons was highest (120% of cell dry weight) at the end of the linear growth phase. fractionation of the lipids using thin-layer chromatography and subsequent gas chromatography showed that hydrocarbons were also obtained follo ... | 2001 | 11549018 |
| genetic analysis of phosphomannomutase/phosphoglucomutase from vibrio furnissii and characterization of its role in virulence. | the pmm gene from vibrio furnissii, which encodes phosphomannomutase (pmm), was cloned and sequenced. the open reading frame consisted of 1,434 bp, encoding a polypeptide of 477 amino acids with a molecular mass of 53,325 da. the predicted amino acid sequence of v. furnissii pmm showed high similarity with pmms from other enteric bacteria, such as v. cholerae, salmonella sp. and escherichia coli. the pmm protein was overexpressed in e. coli as a his(6)-tagged recombinant protein. the estimated a ... | 2003 | 12904831 |
| vibrio pacinii sp. nov., from cultured aquatic organisms. | three strains were isolated from cultured aquatic organisms. they were gram-negative, oxidase-positive, motile, fermentative, arginine dihydrolase-positive, lysine and ornithine decarboxylase-negative and sensitive to vibriostatic agent o/129. these strains differ from other related vibrio species by several phenotypic features, which include acetoin and indole production and utilization of amygdalin and d-mannitol. comparison of 16s rdna sequences showed a close relationship to the recently des ... | 2003 | 13130050 |
| conserved amplification of chemotactic responses through chemoreceptor interactions. | many bacteria concentrate their chemoreceptors at the cell poles. chemoreceptor location is important in escherichia coli, since chemosensory responses are sensitive to receptor proximity. it is not known, however, whether chemotaxis in other bacteria is similarly regulated. to investigate the importance of receptor-receptor interactions in other bacterial species, we synthesized saccharide-bearing multivalent ligands that are designed to cluster relevant chemoreceptors. as has been shown with e ... | 2002 | 12193613 |
| the chitinolytic cascade in vibrios is regulated by chitin oligosaccharides and a two-component chitin catabolic sensor/kinase. | chitin, a highly insoluble polymer of glcnac, is produced in massive quantities in the marine environment. fortunately for survival of aquatic ecosystems, chitin is rapidly catabolized by marine bacteria. here we describe a bacterial two-component hybrid sensor/kinase (of the arcb type) that rigorously controls expression of approximately 50 genes, many involved in chitin degradation. the sensor gene, chis, was identified in vibrio furnissii and vibrio cholerae (predicted amino acid sequences, f ... | 2004 | 14699052 |
| production of alternatives to fuel oil from organic waste by the alkane-producing bacterium, vibrio furnissii m1. | we investigated the production of alternatives to fuel oil through the bacterial metabolism of organic waste. the availability for this purpose of various sources of organic waste for hydrocarbon production by the alkane-producing bacterium, vibrio furnissii m1, was examined. | 2005 | 15659187 |
| new pathway for long-chain n-alkane synthesis via 1-alcohol in vibrio furnissii m1. | alkane biosynthesis in the bacterium vibrio furnissii m1 involves the synthesis of long-chain alkanes via 1-alcohol. evidence for this novel pathway are the following. (i) both even- and odd-carbon-number n-alkanes were produced from glucose, while only even-carbon-number fatty acids were produced in v. furnissii m1. this result cannot be explained by the decarbonylation pathway. (ii) pentadecane and hexadecane were produced from 1-hexadecanoic acid by membrane fractions of v. furnissii m1, and ... | 2005 | 15687207 |
| isolation of a glucosamine-specific kinase, a unique enzyme of vibrio cholerae. | we showed previously that chitin catabolism by the marine bacterium vibrio furnissii involves at least three signal transduction systems and many genes, several of which were molecularly cloned, and the corresponding proteins were characterized. the predicted amino acid sequences of these proteins showed a high degree of identity to the corresponding proteins from vibrio cholerae, whose complete genomic sequence has recently been determined. we have therefore initiated studies with v. cholerae. ... | 2002 | 11850417 |
| cloning, sequences, and characterization of two chitinase genes from the antarctic arthrobacter sp. strain tad20: isolation and partial characterization of the enzymes. | arthrobacter sp. strain tad20, a chitinolytic gram-positive organism, was isolated from the sea bottom along the antarctic ice shell. arthrobacter sp. strain tad20 secretes two major chitinases, chia and chib (archia and archib), in response to chitin induction. a single chromosomal dna fragment containing the genes coding for both chitinases was cloned in escherichia coli. dna sequencing analysis of this fragment revealed two contiguous open reading frames coding for the precursors of archia (8 ... | 2001 | 11160110 |
| genomic and biochemical studies demonstrating the absence of an alkane-producing phenotype in vibrio furnissii m1. | vibrio furnissii m1 was recently reported to biosynthesize n-alkanes when grown on biopolymers, sugars, or organic acids (m. o. park, j. bacteriol. 187:1426-1429, 2005). in the present study, v. furnissii m1 was subjected to genomic analysis and studied biochemically. the sequence of the 16s rrna gene and repetitive pcr showed that v. furnissii m1 was not identical to other v. furnissii strains tested, but the level of relatedness was consistent with its assignment as a v. furnissii strain. puls ... | 2007 | 17921268 |
| molecular characterization of the beta-n-acetylglucosaminidase of escherichia coli and its role in cell wall recycling. | the beta-n-acetylglucosaminidase of escherichia coli was found to have a novel specificity and to be encoded by a gene (nagz) that maps at 25.1 min. it corresponds to an open reading frame, ycfo, whose predicted amino acid sequence is 57% identical to that of vibrio furnissii exoii. nagz hydrolyzes the beta-1,4 glycosidic bond between n-acetylglucosamine and anhydro-n-acetylmuramic acid in cell wall degradation products following their importation into the cell during the process for recycling c ... | 2000 | 10940025 |
| chitin catabolism in the marine bacterium vibrio furnissii. identification, molecular cloning, and characterization of a n, n'-diacetylchitobiose phosphorylase. | the major product of bacterial chitinases is n,n'-diacetylchitobiose or (glcnac)(2). we have previously demonstrated that (glcnac)(2) is taken up unchanged by a specific permease in vibrio furnissii (unlike escherichia coli). it is generally held that marine vibrios further metabolize cytoplasmic (glcnac)(2) by hydrolyzing it to two glcnacs (i.e. a "chitobiase "). here we report instead that v. furnissii expresses a novel phosphorylase. the gene, chbp, was cloned into e. coli; the enzyme, chbp, ... | 2000 | 10913116 |
| chitin catabolism in the marine bacterium vibrio furnissii. identification and molecular cloning of a chitoporin. | chitin catabolism by the marine bacterium vibrio furnissii involves many genes and proteins, including two unique periplasmic hydrolases, a chitodextrinase and a beta-n-acetylglucosaminidase (keyhani, n. o. , and roseman, s. (1996) j. biol. chem. 271, 33414-33424 and 33425-33432). a specific chitoporin in the outer membrane may be required for these glycosidases to be accessible to extracellular chitooligosaccharides, (glcnac)(n), that are produced by chitinases. we report here the identificatio ... | 2000 | 10913115 |
| [development of taqman real-time pcr in detection of aeromonas hydrophila]. | to develop a taqman real-time pcr for the detection of aeromonas hydrophila. | 2009 | 19954074 |
| mechanism of action and identification of asp242 as the catalytic nucleophile of vibrio furnisii n-acetyl-beta-d-glucosaminidase using 2-acetamido-2-deoxy-5-fluoro-alpha-l-idopyranosyl fluoride. | the novel mechanism-based reagent 2-acetamido-2-deoxy-5-fluoro-alpha-l-idopyranosykl fluoride has been synthesized, and the kinetic parameters k(m) = 0.23 mm and k(cat)= 0.55 min(-1) for its hydrolysis by vibrio furnisi beta-n-acetylglucosaminidase (exoii) have been determined. investigation of mixtures of enzyme with this slow substrate by electrospray mass spectrometry revealed a high steady-state population of the 2-acetamido-2-deoxy-5-fluoro-beta-l-idopyranosyl-enzyme, indicating that the hy ... | 2000 | 10625486 |
| complete genome sequence of a free-living vibrio furnissii sp. nov. strain (nctc 11218). | | 2011 | 21217006 |
| additional o antigens of vibrio fluvialis and vibrio furnissii. | a total of 297 strains of vibrio fluvialis and vibrio furrnissii, which were collected from various countries for the past 15-year period of 1984-1998, were serogrouped. of those examined, 239 strains of v. fluvialis and v. furnissii were classified into 29 known o serogroups; 9 strains were found to belong to r-form cultures, and the rest of the 49 strains could not be serogrouped. of those serologically untypable strains, 26 novel o serogroups (o36 to o61) were established and added to our ref ... | 1999 | 10507993 |
| purification, characterization and gene analysis of n-acetylglucosaminidase from enterobacter sp. g-1. | enterobacter sp. g-1 is a bacterium isolated previously as a chitinase-producing bacterium. we found this bacterium also produced n-acetylglucosaminidase and characterized that in this study. extracellular n-acetylglucosaminidase of 92.0 kda was purified near homogeneity by 8.57-fold from enterobacter sp. g-1. the optimum temperature and the optimum ph of the purified n-acetylglucosaminidase was 45 degrees c and 6.0, respectively. the n-terminal amino acid sequence of 23 residues of n-acetylgluc ... | 1999 | 10478453 |
| horizontal transfer of chromosomal dna between the marine bacterium vibrio furnissii and escherichia coli revealed by sequence analysis. | previous in silico analysis of the 67.4-76.0 minutes region of the escherichia coli genome led to the identification of a gene cluster (named aga) comprising five genes encoding homologs of the mannose transporter of e. coli, a member of the sugar-specific phosphoenolypyruvate/sugar phosphotransferase system (pts). in the present work, we compared the aga gene cluster of e. coli, which has been considered to be involved in n-acetylgalactosamine or n-acetylmannosamine transport and metabolism, to ... | 1998 | 9697096 |
| vibrio xiamenensis sp. nov., a novel cellulase-producing bacterium isolated from mangrove soil. | a taxonomic study was carried out on a cellulase-producing bacterium g21t isolated from mangrove soil in xiamen, fujian province of china. cells were gram-negative, slightly curved rods and motile with a single polar flagellum. the strain grew at 15-40°c and in 0.5-10% nacl (w/v). phylogenetic analysis based on 16s rrna gene sequences revealed that strain g21t belonged to genus vibrio and formed a clade with v. furnissii atcc 350116t (sequence similarity, 97.4%), v. fluvialis lmg 7894t (97.1%) a ... | 2011 | 22039001 |
| 4-methylumbelliferyl glycosides of n-acetyl 4-thiochito-oligosaccharides as fluorogenic substrates for chitodextrinase from vibrio furnissii. | the degradation of chitin involves a diverse array of enzymes, some with overlapping substrate specificities. in order to distinguish between different types of enzymes, specific substrates are needed. toward this end, two new fluorogenic substrates containing thio-glycosidic linkages, 4-methylumbelliferyl n,n'-diacetyl-4-thio-beta-chitobioside (mu-tcb) and n,n',n"-triacetyl-4,4'-dithio-beta-chitotrioside (mu-tct) are described. the substitution of the glycosidic oxygens (except the one that lin ... | 1997 | 9376688 |
| antibacterial activity of litsea cubeba (lauraceae, may chang) and its effects on the biological response of common carp cyprinus carpio challenged with aeromonas hydrophila. | the aims of this study were to characterize the antibacterial activity and the chemotype of litsea cubeba leaf essential oil (eo) harvested in north vietnam and to investigate the biological effects induced by the leaf powder on growth, nonspecific immunity and survival of common carp (cyprinus carpio) challenged with aeromonas hydrophila. | 2016 | 27124660 |
| development of a selective and differential medium for capsulated lactococcus garvieae. | a selective and differential medium termed 'lg agar' was developed for the isolation and presumptive identification of lactococcus garvieae that results in black colonies with red halos. in this study, all 14 strains of l. garvieae and only 9 of the 148 strains representing 38 other species were able to grow on the lg agar. the nine viable strains on lg agar plates (including enterococcus faecalis, enterococcus faecium, lactococcus lactis, vibrio fluvialis, vibrio furnissii, vibrio mimicus and v ... | 2014 | 24033791 |
| vibrio furnissii isolated from humans in peru: a possible human pathogen? | during a cholera surveillance programme, vibrio furnissii was isolated in late january and early february 1994 from stool samples collected from 14 persons of whom six had diarrhoea. the remaining eight persons were healthy family members or neighbours to cholera cases. no common source of infection was found. strains isolated from stool samples each showed typical biochemical reactions of v. furnissii including gas production. each isolate, except one, agglutinated o-antisera yielding a total o ... | 1997 | 9363012 |
| release and consumption of d-amino acids during growth of marine prokaryotes. | analysis of the composition of the marine-dissolved organic matter has highlighted the importance of d-amino acids, whose origin is attributed mainly to the remains of bacterial peptidoglycan released as a result of grazing or viral lysis. however, very few studies have focused on the active release of d-amino acids by bacteria. with this purpose, we measured the concentration of dissolved amino acids in both enantiomeric forms with two levels of complexity: axenic cultures of vibrio furnissii a ... | 2014 | 24057323 |
| sugar transport by the marine chitinolytic bacterium vibrio furnissii. molecular cloning and analysis of the mannose/glucose permease. | we have previously reported that the chitin catabolic cascade in vibrio furnissii involves multiple signal transducing systems, and that mono- and disaccharide chemoreceptors/transporters are essential components of some of these systems. this and the accompanying papers (bouma, c. l., and roseman, s. (1996) j. biol. chem 271, 33457-33467; keyhani, n. o., wang, l.-x., lee, y. c., and roseman, s. (1996) j. biol. chem. 271, 33409-33413) describe some of the sugar transporters. a 13-kilobase pair f ... | 1996 | 8969210 |
| sugar transport by the marine chitinolytic bacterium vibrio furnissii. molecular cloning and analysis of the glucose and n-acetylglucosamine permeases. | chitin catabolism by the marine bacterium vibrio furnissii involves chemotaxis to and transport of n-acetyl-d-glucosamine (glcnac) and d-glucose. we report the properties of the respective permeases that complemented e. coli glc- man- mutants. although the v. furnissii glc-specific permease (55,941 da) shares 38% identity with e. coli iiglc (ptsg), it is 67% identical to malx of the e. coli maltose operon (reidl, j., and boos, w. (1991) j. bacteriol. 173, 4862-4876). an adjacent open reading fra ... | 1996 | 8969209 |
| molecular cloning and characterization of a novel beta-n-acetyl-d-glucosaminidase from vibrio furnissii. | the accompanying papers (keyhani, n. o., and roseman, s. (1996) j. biol. chem. 271, 33414-33424; keyhani, n. o., and roseman, s. (1996) j. biol. chem. 271, 33425-33432) describe two unique beta-n-acetylglucosaminidases from vibrio furnissii. a third, exoii, is reported here. the gene, exoii, was cloned into escherichia coli, sequenced, and exoii purified to apparent homogeneity (36 kda). the molecular weight and n-terminal 16 amino acids of the protein conform to the predicted sequence. exoii ex ... | 1996 | 8969206 |
| the chitin catabolic cascade in the marine bacterium vibrio furnissii. molecular cloning, isolation, and characterization of a periplasmic beta-n-acetylglucosaminidase. | we have described some steps in chitin catabolism by vibrio furnissii, and proposed that chitin oligosaccharides are hydrolyzed in the periplasmic space to glcnac and (glcnac)2. since (glcnac)2 is an important inducer in the cascade, it must resist hydrolysis in the periplasm. known v. furnissii periplasmic hydrolases comprise an endoenzyme (keyhani, n. o. and roseman, s. (1996) j. biol. chem. 271, 33414-33424), and the beta-n-acetylglucosaminidase, exoi, reported here. exoi was isolated from a ... | 1996 | 8969205 |
| the chitin catabolic cascade in the marine bacterium vibrio furnissii. molecular cloning, isolation, and characterization of a periplasmic chitodextrinase. | chitin catabolism in vibrio furnissii comprises several signal transducing systems and many proteins. two of these enzymes are periplasmic and convert chitin oligosaccharides to glcnac and (glcnac)2. one of these unique enzymes, a chitodextrinase, designated endoi, is described here. the protein, isolated from a recombinant escherichia coli clone, exhibited (via sds-polyacrylamide gel electrophoresis) two enzymatically active, close running bands ( approximately mass of 120 kda) with identical n ... | 1996 | 8969204 |
| the chitin catabolic cascade in the marine bacterium vibrio furnissii. characterization of an n,n'-diacetyl-chitobiose transport system. | the disaccharide n,n'-diacetyl-chitobiose, (glcnac)2, is critical in chitin dissimilation by vibrio furnissii and, as reported here, is taken up by a specific permease. since (glcnac)2 is rapidly catabolized by v. furnissii, a non-hydrolyzable thioglycoside analogue was used: methyl beta-n,n'-[3h]diacetyl-thiochitobioside (me-[3h]tcb). me-tcb and tcb substitute for (glcnac)2 as chemoattractants and inducers of beta-n-acetylglucosaminidase activity. the [3h]me-tcb uptake system was induced only b ... | 1996 | 8969203 |
| chemotaxis of the marine bacterium vibrio furnissii to sugars. a potential mechanism for initiating the chitin catabolic cascade. | immense quantities of chitin are catabolized by marine bacteria, and this process involves at least three signal transduction systems in vibrio furnissii. one system, chemotaxis to chitin oligosaccharides, is probably used to colonize chitin particles. but how do the first few cells find this highly insoluble polysaccharide? the following hypothesis is proposed to answer this question: the bacteria respond to soluble chemo-attractants in exudates from injured organisms. virtually all chitin-prod ... | 1993 | 8486635 |
| sequence determination of rrna genes of pathogenic vibrio species and whole-cell identification of vibrio vulnificus with rrna-targeted oligonucleotide probes. | a comparative analysis of seven new 16s rrna gene sequences of pathogenic vibrio species with previously published vibrio sequences confirmed that vibrio vulnificus represents a group that is not closely related to the core organisms of the genus vibrio. in addition, we found that v. vulnificus, listonella (vibrio) anguillarum and vibrio diazotrophicus branch off separately from the core group. a comparison of the 16s rrna gene sequences of v. vulnificus strains belonging to biotypes 1 and 2 rev ... | 1994 | 8186099 |
| laboratory evaluation on pathogenic potentialities of vibrio furnissii. | sixteen strains of vibrio furnissii recovered from 16 brazilian patients with diarrhea were screened for virulence-associated factors. all strains were non-invasive, non-fimbriated, and did not produce either enterotoxins or cholera-like toxin. in contrast, most were hemolytic on blood agar and their broth-culture supernatants damaged hela cell monolayers. these cytolysins, as accepted for other enteropathogenic members of the family vibrionaceae, might be determinants of pathogenicity in v. fur ... | 1993 | 8139467 |
| extracellular and surface-bound biological activities of vibrio fluvialis, vibrio furnissii and related species. | twenty-seven vibrio strains were assessed for virulence-associated biological activities, including iron chelation, hydrolases, haemolysis and haemagglutination. all strains hydrolysed dna, chitin, gelatin and casein, produced siderophores, and lysed red blood cells. all v. fluvialis, v. cholerae and v. mimicus strains exhibited diverse lipolytic activity distinct from more discriminate lipolysis by v. furnissii. v. furnissii manifested fibrin and mucin hydrolysis but no phosphate or esculin hyd ... | 1989 | 2779486 |
| molecular epidemiologic evidence for association of thermostable direct hemolysin (tdh) and tdh-related hemolysin of vibrio parahaemolyticus with gastroenteritis. | the kanagawa phenomenon induced by the thermostable direct hemolysin (tdh) of vibrio parahaemolyticus is almost exclusively associated with clinical strains, and tdh has been considered an important virulence factor. however, kanagawa phenomenon-negative strains isolated from patients with diarrhea have recently been shown to produce tdh-related hemolysin (trh). we studied the distribution of the tdh gene encoding tdh and the trh gene encoding trh in vibrios by hybridization analyses. the presen ... | 1990 | 2228229 |
| chitin utilization by marine bacteria. chemotaxis to chitin oligosaccharides by vibrio furnissii. | the adhesion/deadhesion apparatus of the marine bacterium vibrio furnissii (yu, c., lee, a., bassler, b. l., and roseman, s. (1991) j. biol. chem. 266, 24260-24267) probably catalyzes the first step in colonizing chitin. evidence is presented here for a second step, chemotaxis to chitin hydrolysis products. v. furnissii swarms toward chitin oligomers (glcnac)n, n = 1-6, at initial concentrations as low as 10 microm. a modified capillary assay was used for quantitation; the cells exhibit low leve ... | 1991 | 1761532 |
| reaction mechanism of chitobiose phosphorylase from vibrio proteolyticus: identification of family 36 glycosyltransferase in vibrio. | a family 36 glycosyltransferase gene was cloned from vibrio proteolyticus. the deduced amino acid sequence showed a high degree of identity with chbp (chitobiose phosphorylase) from another species, vibrio furnissii. the recombinant enzyme catalysed the reversible phosphorolysis of (glcnac)2 (chitobiose) to form 2-acetamide-2-deoxy-alpha-d-glucose 1-phosphate [glcnac-1-p] and glcnac, but showed no activity on cellobiose, indicating that the enzyme was chbp, not cellobiose phosphorylase. in the s ... | 2004 | 13678418 |
| detailed comparative analysis of the catalytic mechanisms of beta-n-acetylglucosaminidases from families 3 and 20 of glycoside hydrolases. | beta-n-acetylglucosaminidases are commonly occurring enzymes involved in the degradation of polysaccharides and glycoconjugates containing n-acetylglucosamine residues. such enzymes have been classified into glycoside hydrolase families 3 and 20 and are believed to follow distinct chemical mechanisms. family 3 enzymes are thought to follow a standard retaining mechanism involving a covalent glycosyl enzyme intermediate while family 20 enzymes carry out a substrate-assisted mechanism involving th ... | 2005 | 16171396 |
| identification of a vibrio furnissii oligopeptide permease and characterization of its in vitro hemolytic activity. | we describe purification and characterization of an oligopeptide permease protein (hly-oppa) from vibrio furnissii that has multifaceted functions in solute binding, in in vitro hemolysis, in antibiotic resistance, and as a virulence factor in bacterial pathogenesis. the solute-binding function was revealed by n-terminal and internal peptide sequences of the purified protein and was confirmed by discernible effects on oligopeptide binding, by accumulation of fluorescent substrates, and by fluore ... | 2007 | 17873048 |
| isolation and characterization of mucous exopolysaccharide (eps) produced by vibrio furnissii strain vb0s3. | marine bacterial strains were isolated from coastal regions of goa and screened for the strains that produce the highest amount of mucous exopolysaccharide (eps). our screening resulted in the identification of the strain vibrio furnissii vb0s3 (hereafter called vb0s3), as it produced the highest eps in batch cultures during the late logarithmic growth phase. the isolate was identified as vb0s3 based on morphological and biochemical properties. growth and eps production were studied in mineral s ... | 2007 | 18051352 |
| identification of vibrio cholerae and vibrio mimicus by multilocus sequence analysis (mlsa). | vibrio cholerae and vibrio mimicus have similar phenotypes and genomes making rapid differentiation of these two species difficult. the first standard multilocus sequence analysis (mlsa) scheme for the identification of these species is described. a collection of 45 representative isolates from different geographical regions and hosts was examined using segments of the housekeeping genes pyrh, reca and rpoa. overall, the closest phylogenetic neighbours of these species were vibrio furnissii and ... | 2008 | 18319466 |
| vibrio porteresiae sp. nov., a diazotrophic bacterium isolated from a mangrove-associated wild rice (porteresia coarctata tateoka). | two facultatively anaerobic, nitrogen-fixing bacteria (strains mssrf30(t) and mssrf31) were isolated from a mangrove-associated wild rice (porteresia coarctata tateoka). these strains were determined to be nitrogen-fixers using the acetylene reduction assay and by pcr detection of a nifh gene amplicon. phylogenetic analyses based on 16s rrna gene sequences indicated that the novel strains were most closely related to vibrio fluvialis lmg 7894(t) (96.8 % gene sequence similarity), vibrio furnissi ... | 2008 | 18599703 |
| isolation and characterization of a virulent vibrio sp. bacterium from clams (meretrix meretrix) with mass mortality. | mm5 was a bacterial strain isolated from moribund clam (meretrix meretrix) collected from a farm with mass mortality outbreak. primary genotypic and phenotypic identification including 16s rdna sequence analysis, multilocus sequence analysis (mlsa) of four housekeeping genes (gapa, ftsz, mreb and topa) and biochemical tests suggested that strain mm5 was a vibrio species closest to but different from vibrio furnissii. our previous study indicated that mm5 could induce a high mortality of m. meret ... | 2010 | 21055407 |
| vibrio furnissii: an unusual cause of bacteremia and skin lesions after ingestion of seafood. | vibrio furnissii in the blood is rarely reported, which may explain why clinical features of bloodstream infections with this organism have not been described. we describe a patient who developed skin lesions and v. furnissii bacteremia and was successfully treated with fluoroquinolones. v. furnissii may be a serious pathogen in patients with underlying comorbidities who are exposed to seafood. | 2011 | 21450956 |
| identification of genes, desr and desa, required for utilization of desferrioxamine b as a xenosiderophore in vibrio furnissii. | we found that vibrio (v.) furnissii atcc35016 can gain iron through a xenosiderophore desferrioxamine b (dfob) for its growth under iron-limiting conditions, concurrent with the expression of the 79-kda iron-repressible outer membrane protein (iromp) in response to the presence of dfob. based on the sequence of the ferrioxamine b (an iron-bound form of dfob) receptor gene in v. vulnificus, two v. furnissii genes, termed desa and desr, encoding the 79-kda iromp and arac-type transcriptional regul ... | 2011 | 21467648 |
| heterodisaccharide 4-o-(n-acetyl-β-d-glucosaminyl)-d-glucosamine is an effective chemotactic attractant for vibrio bacteria that produce chitin oligosaccharide deacetylase. | aims: to investigate the attractant effect of 4-o-(n-acetyl-β-d-glucosaminyl)-d-glucosamine (glcnac-glcn) in the chemotaxis of vibrio bacteria that produce carbohydrate esterase (ce) family 4 chitin oligosaccharide deacetylase (cod), an enzyme that catalyzes the production of glcnac-glcn from n,n'-diacetylchitobiose (glcnac)(2) . methods and results: the chemotactic effect of disaccharides from chitin on several strains of vibrio bacteria was investigated using an agar gel lane-migration metho ... | 2011 | 21575022 |
| comparative analysis of glycoside hydrolases activities from phylogenetically diverse marine bacteria of the genus arenibacter. | a total of 16 marine strains belonging to the genus arenibacter, recovered from diverse microbial communities associated with various marine habitats and collected from different locations, were evaluated in degradation of natural polysaccharides and chromogenic glycosides. most strains were affiliated with five recognized species, and some presented three new species within the genus arenibacter. no strains contained enzymes depolymerizing polysaccharides, but synthesized a wide spectrum of gly ... | 2013 | 23752354 |
| isolation and characterization of biosurfactant producing bacteria from persian gulf (bushehr provenance). | biosurfactants are surface active materials that are produced by some microorganisms. these molecules increase biodegradation of insoluble pollutants. in this study sediments and seawater samples were collected from the coastline of bushehr provenance in the persian gulf and their biosurfactant producing bacteria were isolated. biosurfactant producing bacteria were isolated by using an enrichment method in bushnell-hass medium with diesel oil as the sole carbon source. five screening tests were ... | 2014 | 25037876 |
| influence of niche-specific nutrients on secondary metabolism in vibrionaceae. | many factors, such as the substrate and the growth phase, influence biosynthesis of secondary metabolites in microorganisms. therefore, it is crucial to consider these factors when establishing a bioprospecting strategy. mimicking the conditions of the natural environment has been suggested as a means of inducing or influencing microbial secondary metabolite production. the purpose of the present study was to determine how the bioactivity of vibrionaceae was influenced by carbon sources typical ... | 2016 | 27129958 |
| evaluation of molecular methods to discriminate the closely related species vibrio fluvialis and vibrio furnissii. | vibrio furnissii and vibrio fluvialis are two closely related species which are regarded as emerging human pathogens. human infections have been mainly associated with consumption of seafood or drinking of contaminated water. v. furnissii strains can be distinguished from v. fluvialis by their ability to produce gas from fermentation of carbohydrates. in this study, we compare two phenotypic (biochemical testing and matrix-assisted laser desorption/ionisation time of flight mass spectrometry, ma ... | 2014 | 25242722 |
| vibrio alfacsensis sp. nov., isolated from marine organisms. | five strains (caim 1831(t), caim 1832, caim 1833, caim 1834 and caim 1836) were isolated from cultured sole (solea senegalensis) in two regions of spain, two strains (caim 404 and caim 1294) from wild-caught spotted rose snapper (lutjanus guttatus) in mexico, and one strain (caim 1835) from corals in brazil. the 16s rrna gene sequences of the novel isolates showed similarity to vibrio ponticus (98.2-98.3%, genbank accession no. aj630103) and to a lesser degree to vibrio furnissii (97.2-97.3%, x7 ... | 2012 | 22286904 |
| genome-wide phylogenetic analysis of the pathogenic potential of vibrio furnissii. | we recently reported the genome sequence of a free-living strain of vibrio furnissii (nctc 11218) harvested from an estuarine environment. v. furnissii is a widespread, free-living proteobacterium and emerging pathogen that can cause acute gastroenteritis in humans and lethal zoonoses in aquatic invertebrates, including farmed crustaceans and molluscs. here we present the analyses to assess the potential pathogenic impact of v. furnissii. we compared the complete genome of v. furnissii with 8 ot ... | 2014 | 25191313 |