| nucleotide sequences and characterization of haemolysin genes from aeromonas hydrophila and aeromonas sobria. | extracellular haemolysin is thought to be one of the important virulence factors in aeromonas infection. two extracellular haemolysin genes (ahh3 and ahh4) from aeromonas hydrophila strain 28sa, one (ahh5) from a. hydrophila strain ah-1 and one (asa1) from aeromonas sobria strain 33 were cloned into cosmid and plasmid vector dna in escherichia coli. the nucleotide sequences of the open reading frames of ahh3 and ahh4 are both 1476 basepairs (bp), whereas ahh5 and asa1 are 1455 and 1467 bp in len ... | 1992 | 1302284 |
| molecular studies on the aerolysin gene of aeromonas species and discovery of a species-specific probe for aeromonas trota species nova. | a large group of aeromonads and other enteric microorganisms were assayed for the presence of the aerolysin gene with use of dna-dna hybridization. two dna fragments corresponding to the regulatory region (aerc) and the structural gene (aera) were used as probes for the detection of the aerolysin gene in these strains. sequences corresponding to the aerolysin structural gene were widespread among aeromonas isolates. in contrast, the aerc probe was much more selective, and sequences corresponding ... | 1992 | 1600007 |
| aeromonas trota sp. nov., an ampicillin-susceptible species isolated from clinical specimens. | previous dna hybridization studies established 12 aeromonas genospecies, from which nine phenotypic species have been proposed: aeromonas hydrophila, a. sobria, a. caviae, a. media, a. veronii, a. schubertii, a. salmonicida, a. eucrenophila, and a. jandaei. we have delineated a new aeromonas genospecies, a. trota, on the basis of 13 strains isolated primarily from fecal specimens from southern and southeastern asia. all strains were highly related to the proposed type strain, ah2 (atcc 49657t): ... | 1991 | 1864939 |
| studies on aerolysin and a serine protease from aeromonas trota sp. nov. | hybridization of 257 mesophilic aeromonads revealed that the aerolysin gene is present in virtually all strains irrespective of origin of isolation. a probe comprising the promotor region was specific for a species now defined as aeromonas trota sp. nov. finally, isolation of a serine protease that is concomitantly expressed with aerolysin is described. | 1991 | 2044688 |
| aeromonas enteropelogenes and aeromonas ichthiosmia are identical to aeromonas trota and aeromonas veronii, respectively, as revealed by small-subunit rrna sequence analysis. | the 16s rrna gene sequences of the type strains of aeromonas enteropelogenes and aeromonas ichthiosmia were determined by polymerase chain reaction direct sequencing in order to clarify their interrelationships with other aeromonad species. on the basis of 16s rrna gene sequence analysis, a. enteropelogenes and a. ichthiosmia were found to be identical to aeromonas trota and aeromonas veronii, respectively. | 1993 | 8240968 |
| presence of aeromonas trota in aquatic environment. | | 1995 | 8551948 |
| characterization of aeromonas trota strains that cross-react with vibrio cholerae o139 bengal. | it has previously been shown that vibrio cholerae o139 bengal shares antigens with v. cholerae serogroups o22 and o155. we detected six surface water isolates of aeromonas trota that agglutinated in polyclonal antisera to v. cholerae o139 and v. cholerae o22 but not in antiserum to v. cholerae o155. on the basis of agglutinin-absorption studies, the antigenic relationship between the cross-reacting bacteria were found to be in an a,b-a,c fashion, where a is the common antigenic epitope and b and ... | 1995 | 8586685 |
| gastroenteritis caused by aeromonas trota in a child. | a case of acute diarrhoea caused by aeromonas trota (formerly hg 13 group) in a spanish child is reported. the strain was isolated in the faeces using the cin agar (cefsulodin-irgasan-novobiocin) culture media. the strain was initially identified as a sobria by the commercial gni card and api 20e biochemical systems. the strain was, however, vogesproskauer and sucrose negative, so complementary tests of cellobiose fermentation and gluconate oxidation were performed. these tests, together with th ... | 1996 | 8655689 |
| structure of the o-specific polysaccharide of an aeromonas trota strain cross-reactive with vibrio cholerae o139 bengal. | the o-specific polysaccharide of an aeromonas trota strain was isolated by hydrolysis of the lipopolysaccharide at ph 4.5 followed by gel-permeation chromatography and found to consist of hexasaccharide repeating units containing d-galactose, l-rhamnose, 3,6-dideoxy-l-xylo-hexose (colitose, col), 2-acetamido-2-deoxy-d-glucose and 2-acetamido-2-deoxy-d-galactose in the ratios 1:1:2:1:1. partial hydrolysis of the polysaccharide with 48% hydrofluoric acid resulted in selective removal of colitose t ... | 1996 | 8665933 |
| aeromonas trota strains, which agglutinate with vibrio cholerae o139 bengal antiserum, possess a serologically distinct fimbrial colonization factor. | pili of aeromonas trota strain 1220, which agglutinates with vibrio cholerae o139 bengal antiserum, were purified and characterized. the molecular mass of the subunit protein was estimated to be 20 kda and the pl was 5 center dot 4. the pili were immunologically unrelated to the other aeromonas pili reported so far. however, the n-terminal amino acid sequence of the subunit pilin was similar to those of the pilins from other aeromonas pili reported previously. neither a. trota cells nor pili pur ... | 1996 | 8932704 |
| high-resolution genotypic analysis of the genus aeromonas by aflp fingerprinting. | we investigated the ability of a recently developed genomic fingerprinting technique, named aflp, to differentiate the 14 currently defined dna hybridization groups (hgs) in the genus aeromonas. we also determined the taxonomic positions of the phenospecies aeromonas allosaccharophila, aeromonas encheleia, aeromonas enteropelogenes, and aeromonas ichthiosmia, which have not been assigned to hgs yet. a total of 98 aeromonas type and reference strains were included in this study. for the aflp anal ... | 1996 | 8934910 |
| rapid and sensitive method for the detection of aeromonas caviae and aeromonas trota by polymerase chain reaction. | a 16s rdna-based polymerase chain reaction (pcr) method was developed for the detection of aeromonas caviae and aeromonas trota. these two species were identified from other aeromonas spp. and closely related species by primers set (aer1 and aer2). the amplified product was 316 bp. the identity of the amplified product was confirmed by dna-dna hybridization. two sets of primers (aer8 and aer9) were used for specific identification of aer. caviae. amplifying the 260 bp fragment of 16s rrna gene r ... | 1997 | 9134769 |
| production of haemolysin and enterotoxin by aeromonas jandaei and aeromonas trota strains after animal passage. | five aeromonas jandaei and 12 aeromonas trota isolates were tested for the production of haemolysin and enterotoxin, and the correlation between these two properties. the majority (10 isolates) of the strains produced beta-haemolysis. the titres of haemolytic activity for both species were 8-64 hu/ml. in the initial ileal loop test, only two (a. trota) of the 17 isolates produced enterotoxin. one each of these 2 a. trota strains was beta-haemolytic and non-haemolytic. the remaining isolates of a ... | 1996 | 9203791 |
| molecular cloning, nucleotide sequence, and expression in escherichia coli of a hemolytic toxin (aerolysin) gene from aeromonas trota. | aeromonas trota ak2, which was derived from atcc 49659 and produces the extracellular pore-forming hemolytic toxin aerolysin, was mutagenized with the transposon mini-tn5km1 to generate a hemolysin-deficient mutant, designated strain ak253. southern blotting data indicated that an 8.7-kb noti fragment of the genomic dna of strain ak253 contained the kanamycin resistance gene of mini-tn5km1. the 8.7-kb noti dna fragment was cloned into the vector pgem5zf(-) by selecting for kanamycin resistance, ... | 1998 | 9647817 |
| [new species of aeromonas isolated in cuba]. | one hundred and fifty-five strains of aeromonas isolated in the stools of children under 5 years presenting with acute diarrheal disease were studied. using the aerokey ii system for the identification of species, 47 strains were identified as aeromonas caviae, 58 as aeromonas hydrophila, 23 as aeromonas veronii biovar sobria, 14 as aeromonas trota, 9 as aeromonas veronii biovar veronii, 2 as aeromonas jandaei and 2 as aeromonas shubertii, emphasis is placed on the advantages of this method whic ... | 1995 | 9813480 |
| identification of aeromonas trota (hybridization group 13) by amplification of the aerolysin gene using polymerase chain reaction. | aeromonas trota is recognized as an important enteropathogen, and its haemolysin (aerolysin) is purported to be one of the virulence factors. rapid detection and identification of a. trota is important for early and specific diagnosis of the infectious diseases that it causes. synthetic oligonucleotide primers were used in a polymerase chain reaction (pcr) technique to amplify a species-specific sequence of the aera gene, which encodes the aerolysin of a. trota. a dna fragment of 622 bp was ampl ... | 1999 | 10208799 |
| dna-dna reassociation and phenotypic data indicate synonymy between aeromonas enteropelogenes schubert et al. 1990 and aeromonas trota carnahan et al. 1991. | mainly on the basis of phylogenetic and genotypic evidence, it has been suggested previously that the species aeromonas enteropelogenes schubert et al. 1990 is identical to the species aeromonas trota carnahan et al. 1991. probably because the description of a. enteropelogenes preceded the proposal of a. trota by only a few months, dna-dna hybridizations were never performed between representative strains of these two taxa. in the present study, new dna-dna hybridizations between the type strain ... | 2002 | 12508855 |
| genetic linkage of the penicillinase gene, amp, and blrab, encoding the regulator of beta-lactamase expression in aeromonas spp. | aeromonas hydrophila t429125, a human clinical isolate, possesses three coordinately inducible beta-lactamases encoded by amph (class d beta-lactamase), ceph (class c beta-lactamase) and imih (class b beta-lactamase). we report that upstream of amph there are two genes, blra and blrb, encoding a putative two-component regulatory system. pcr studies revealed the same blrab-amp gene arrangement in all aeromonas spp. isolates tested; namely, aeromonas veronii bv. sobria, aeromonas jandaei, aeromona ... | 2003 | 12746371 |
| aeromonas simiae sp. nov., isolated from monkey faeces. | two aeromonas strains, ibs s6874(t) and ibs s6652, were isolated from the faeces of two healthy monkeys (macaca fascicularis) from mauritius that were kept in quarantine in the centre for primatology, strasbourg, france. phylogenetic analysis based on 16s rrna gene sequences showed that the two isolates formed an unknown genetic lineage within the genus aeromonas. the two isolates had nearly identical sequences (0.1 % nucleotide substitution) that were related closely to those of recognized aero ... | 2004 | 15023964 |
| antimicrobial activity of vanillin against spoilage microorganisms in stored fresh-cut mangoes. | the antimicrobial activity of vanillin against four bacteria (pantoea agglomerans, aeromonas enteropelogenes, micrococcus lylae, and sphingobacterium spiritovorun), four fungi (alternaria sp., aspergillus sp., penicillium sp., and fusarium sp.), and three unidentified yeasts isolated from spoiling fresh-cut mango slices was verified in laboratory media adjusted to ph 5.0. mics of vanillin against the fungi (12.5 to 13.3 mm), bacteria (10 to 13.3 mm), and yeasts (5.0 to 6.7 mm) indicated that all ... | 2006 | 16865911 |
| biochemical and molecular characterization of tetracycline-resistant aeromonas veronii isolates from catfish. | eighty-one tetracycline-resistant aeromonas sp. strains were isolated from farm-raised catfish. morphological and biochemical characteristics indicated that 23 of the 81 aeromonads were aeromonas hydrophila, 7 isolates were aeromonas trota, 6 isolates were aeromonas caviae, 42 isolates were aeromonas veronii, and 3 isolates were aeromonas jandaei. however, the alui and mboi restriction fragment length polymorphism (rflp) patterns of the pcr-amplified 1.4-kb 16s rrna gene from all 81 tetracycline ... | 2006 | 17021193 |
| wound infection and septic shock due to aeromonas trota in a patient with liver cirrhosis. | | 2007 | 17479957 |
| sampling bias created by ampicillin in isolation media for aeromonas. | members of the bacterial genus aeromonas are widely isolated from aquatic environments and studied in part for their ability to act as opportunistic pathogens in a variety of animals. all aeromonads, with the exception of aeromonas trota, are generally thought to be resistant to ampicillin, so the antibiotic is frequently added to isolation medium as a selective agent. in this study, 282 aeromonads from environmental sources were isolated on a medium without ampicillin and their resistance to am ... | 2007 | 17496948 |
| genetic and biochemical characterization of tru-1, the endogenous class c beta-lactamase from aeromonas enteropelogenes. | aeromonas enteropelogenes (formerly a. tructi) was described to be an ampicillin-susceptible and cephalothin-resistant aeromonas species, which suggests the production of a cephalosporinase. strain atcc 49803 was susceptible to amoxicillin, cefotaxime, and imipenem but resistant to cefazolin (mics of 2, 0.032, 0.125, and >256 microg/ml, respectively) and produced an inducible beta-lactamase. cefotaxime-resistant mutants (mic, 32 microg/ml) that showed constitutive beta-lactamase production could ... | 2010 | 20124004 |
| an acute gastroenteritis outbreak of vibrio parahaemolyticus o4:k55 in nursing college, thailand. | a cluster of acute gastroenteritis among nursing students was noticed on 13th september 2005. between 13th and 17th september 2005, a retrospective cohort study was then conducted to identify the most likely cause of gastroenteritis at a nursing college in bangkok, thailand. self-administered questionnaires, interviews, environmental investigations, and rectal swabs from all participants were carried out. in the investigation, 98.9% female nursing students were investigated and had completed the ... | 2010 | 20962724 |
| aqu-1, a chromosomal class c β-lactamase, among clinical aeromonas dhakensis isolates: distribution and clinical significance. | aeromonas dhakensis, a recently described aeromonas sp. formerly called aeromonas aquariorum, is associated with human infections. in this study, a chromosomal gene, blaaqu-1, was identified in a. dhakensis aak1 that constitutes a 1143-bp open reading frame and is 87% identical to the gene encoding ceph in aeromonas hydrophila. an escherichia coli top10 cell transformant harbouring blaaqu-1 was resistant to cefotaxime but not to cefepime. mrna expression of blaaqu-1 in the cefotaxime-resistant m ... | 2013 | 24055254 |
| potential enterotoxicity and antimicrobial resistance pattern of aeromonas species isolated from pet turtles and their environment. | to investigate the potential enterotoxicity and antimicrobial resistance of aeromonads from pet turtles as a risk for human infection, one hundred and two aeromonas spp. were isolated from the feces, skin and rearing environments of pet turtles and identified by biochemical and gyrb sequence analyses. aeromonas enteropelogenes was the predominant species among the isolates (52.9%) followed by a. hydrophila (32.4%), a. dharkensis (5.9%), a. veronii (4.9%) and a. caviae (3.9%). their potential ent ... | 2017 | 28392536 |
| properties of hemolysin and protease produced by aeromonas trota. | we examined the properties of exotoxins produced by aeromonas trota (a. enteropelogenes), one of the diarrheagenic species of aeromonadaceae. nine of 19 a. trota isolates that grew on solid media containing erythrocytes showed hemolytic activity. however, the hemolytic activities of the culture supernatants of these hemolytic strains of a. trota were markedly lower than those of a. sobria when cultured in liquid medium, and the amount of hemolysin detected by immunoblotting using antiserum again ... | 2014 | 24633045 |
| prevalence and characterisation of quinolone resistance genes in aeromonas spp. isolated from pet turtles in south korea. | the aim of this study was to characterise aeromonas spp. isolated from popular species of pet turtle to assess the potential risk of pet turtles as a source of target gene alterations in the quinolone resistance-determining region (qrdr) and transferable plasmid-mediated quinolone resistance (pmqr) genes. | 2017 | 28743647 |
| characteristics of an aeromonas trota strain isolated from cerebrospinal fluid. | aeromonas are ubiquitous in aquatic habitats. however some species can cause infections in humans, but rarely meningitis. here we describe the isolation and characterization of an aeromonas strain from cerebrospinal fluid of a meningitis patient. the isolate, identified as a. trota by biochemical and molecular methods, was susceptible to ampicillin but resistant to cephalothin and cefazolin. genome sequencing revealed virulence factor genes such as type vi secretion system, aerolysin and lateral ... | 2018 | 29355700 |
| antibiotic resistance and virulence traits of bacterial pathogens from infected freshwater fish, labeo rohita. | bacterial infectious diseases are a main dangerous problem in aquaculture farming. it causes multiple diseases in fish as well as in human being and it has considerable virulence potential. in this connection, the moot of study focus to discriminate bacterial isolates recovered from naturally diseased labeo rohita fish and their virulent characteristics. based on the β-haemolysis factor, four isolates (kadr11, kadr12, kadr13 and kadr14) were selected for further delineation. these bacterial isol ... | 2018 | 29339308 |