| [structure of the yersinia rohdei o-specific polysaccharide]. | an o-specific polysaccharide composed of l-rhamnose was obtained on mild acid degradation of the yersinia rohdei lipopolysaccharide. on the basis of methylation studies and 13c nmr data, the o-specific polysaccharide is shown to be a linear l-rhamnan built up of trisaccharide repeating units of the following structure: -->3)-alpha-l-rhap-(1-->3)-alpha-l-rhap-(1-->3)-beta-l-rh ap-(1--> the structure was confirmed by calculations with the use of the known glycosylation effect on 13c chemical shift ... | 1993 | 7690568 |
| a novel phytase from yersinia rohdei with high phytate hydrolysis activity under low ph and strong pepsin conditions. | two novel phytase genes belonging to the histidine acid phosphatase family were cloned from yersinia rohdei and y. pestis and expressed in pichia pastoris. both the recombinant phytases had high activity at ph 1.5-6.0 (optimum ph 4.5) with an optimum temperature of 55 degrees c. compared with the major commercial phytases from aspergillus niger, escherichia coli, and a potential commercial phytase from y. intermedia, the y. rohdei phytase was more resistant to pepsin, retained more activity unde ... | 2008 | 18548246 |
| deconvolution and database search of complex tandem mass spectra of intact proteins: a combinatorial approach. | top-down proteomics studies intact proteins, enabling new opportunities for analyzing post-translational modifications. because tandem mass spectra of intact proteins are very complex, spectral deconvolution (grouping peaks into isotopomer envelopes) is a key initial stage for their interpretation. in such spectra, isotopomer envelopes of different protein fragments span overlapping regions on the m/z axis and even share spectral peaks. this raises both pattern recognition and combinatorial chal ... | 2010 | 20855543 |
| changes in fluorescence spectra of bioaerosols exposed to ozone in a laboratory reaction chamber to simulate atmospheric aging. | a laboratory system for exposing aerosol particles to ozone and rapidly measuring the subsequent changes in their single-particle fluorescence is reported. the system consists of a rotating drum chamber and a single-particle fluorescence spectrometer (spfs) utilizing excitation at 263 nm. measurements made with this system show preliminary results on the ultra-violet laser-induced-fluorescence (uv-lif) spectra of single aerosolized particles of yersinia rohdei, and of ms2 (bacteriophage) exposed ... | 2012 | 23388813 |
| n-glycosylation improves the pepsin resistance of histidine acid phosphatase phytases by enhancing their stability at acidic phs and reducing pepsin's accessibility to its cleavage sites. | n-glycosylation can modulate enzyme structure and function. in this study, we identified two pepsin-resistant histidine acid phosphatase (hap) phytases from yersinia kristensenii (ykappa) and yersinia rohdei (yrappa), each having an n-glycosylation motif, and one pepsin-sensitive hap phytase from yersinia enterocolitica (yeappa) that lacked an n-glycosylation site. site-directed mutagenesis was employed to construct mutants by altering the n-glycosylation status of each enzyme, and the mutant an ... | 2016 | 26637601 |
| spectrally-resolved fluorescence cross sections of aerosolized biological live agents and simulants using five excitation wavelengths in a bsl-3 laboratory. | a system for measuring spectrally-resolved fluorescence cross sections of single bioaerosol particles has been developed and employed in a biological safety level 3 (bsl-3) facility at edgewood chemical and biological center (ecbc). it is used to aerosolize the slurry or solution of live agents and surrogates into dried micron-size particles, and to measure the fluorescence spectra and sizes of the particles one at a time. spectrally-resolved fluorescence cross sections were measured for (1) bac ... | 2014 | 24718194 |
| genotypic and phenotypic virulence characteristics and antimicrobial resistance of yersinia spp. isolated from meat and milk products. | a total of 300 food samples including 180 milk and 120 meat products have been examined for the presence of yersinia spp. using the iso 10273 and the cold enrichment method. the overall prevalence of yersinia spp. was 84 (28%). yersinia enterocolitica was isolated from 18 (6%) of the 300 samples. the other yersinia species were detected in the samples yersinia rohdei 15 (5%), yersinia intermedia 14 (4.7%), yersinia pseudotuberculosis 12 (4%), yersinia ruckeri 12 (4%), yersinia mollaretii 5 (1.7% ... | 2015 | 25969137 |