| blastogenic response of human lymphocytes to oral bacterial antigens: comparison of individuals with periodontal disease to normal and edentulous subjects. | cell-mediated immunity in humans to antigens derived from oral plaque bacteria was investigated by using the lymphocyte blastogenesis assay. subjects with varying severities of periodontal disease including normal, gingivitis, periodontitis, and edentulous were compared. mononuclear leukocytes were separated from peripheral blood and cultured with antigens prepared by sonication of actinomyces viscosus (av), actinomyces naeslundii (an), veillonella alcalescens (va), leptotrichia buccalis (lb), b ... | 1976 | 10259 |
| purification and characterization of acetate kinase from veillonella alcalescens atcc 17748. | | 1978 | 30411 |
| oxygen metabolism by the anaerobic bacterium veillonella alcalescens. | | 1978 | 31846 |
| the presence of antibody-coated anaerobic bacteria in asymptomatic bacteriuria during pregnancy. | quantitative anaerobic culture of urine samples obtained from 593 pregnant women by suprapubic bladder aspiration was performed to establish the involvement of anaerobic bacteria in asymptomatic urinary tract infections. the fluorescent antibody (fa) test was applied to the sediments of bladder aspirates to determine the site of infection. anaerobic bacteriuria (greater than or equal to 10(4) microorganisms/ml of urine) was found in 34 patients, of whom five were fa-positive. these anaerobes wer ... | 1979 | 118996 |
| human complement activation by lipopolysaccharides from bacteroides oralis, fusobacterium nucleatum, and veillonella parvula. | the properties of different lipopolysaccharide (lps) preparations to induce c3 conversion in human serum was studied by means of crossed immunoelectrophoresis. c3 conversion by the alternative pathway was evaluated after calcium depletion, and lipid a-dependent activation was measured by means of inhibition with polymyxin b sulfate. lps from bacteroides oralis converted co mainly via the alternative pathway, whereas lps from fusobacterium nucleatum and veillonella parvula const pronounced lipid ... | 1979 | 121108 |
| properties of membrane adenosine triphosphatase of the obligately anaerobic bacterium veillonella alcalescens. | some properties of membrane atpase activity in veillonella alcalescens were examined. mg2+ is required for the activity of the enzyme, and ca2+ also activates the enzyme to some degree. of the nucleotide triphosphates, gtp and itp were hydrolyzed to a lesser extent than atp. the apparent km for atp hydrolysis was 0.25 to 0.63 mm. adp inhibited the enzyme and the kinetic data of its inhibition showed that the presence of adp resulted in positive cooperativity. the enzyme activity was strongly inh ... | 1978 | 149119 |
| amino acid transport in membrane vesicles of obligately anaerobic veillonella alcalescens. | membrane vesicles of veillonella alcalescens, grown in the presence of l-lactate and kno-3, actively transport amino acids under anaerobic conditions in the presence of several electron donors and the electron acceptor nitrate. the highest initial rates of uptake are obtained with l-lactate, followed by reduced nicotinamide adenine dinucleotide, glycerol-1-phosphate, formate, and l-malate.. the membrane vesicles contain the dehydrogenases for these electron donors, and these enzymes are coupled ... | 1975 | 164433 |
| acetate kinase from veillonella alcalescens. regulation of enzyme activity by succinate and substrates. | acetate kinase of veillonella alcalescens has been shown to be highly regulated enzyme exhibiting two levels of control: the requirement for succinate as a heterotropic allosteric effector, and cooperative binding at the substrate level. succinate addition was necessary for enzymatic activity in both the direction of acyl phosphate synthesis and that of atp synthesis. control at the substrate level was apparent in the cooperative binding (hill coefficients of 2) of acetyl phosphate, atp, and adp ... | 1976 | 178662 |
| studies by electron-paramagnetic-resonance spectroscopy on the mechanism of action of xanthine dehydrogenase from veillonella alcalescens. | e.p.r- (electron-paramagnetic-resonance) spectroscopy was used to compare chemical environment and reactivity of molybdenum, flavin and iron-sulphur centres in the enzyme xanthine dehydrogenase from veillonella alcalescens (micrococcus lactilyticus) with those of the corresponding centres in milk xanthine oxidase. the dehydrogenase is frequently contaminated with small but variable amounts of a species resistant to oxidation and giving a new molybdenum (v) e.p.r. signal, "resting i". there is al ... | 1976 | 179532 |
| studies by electron-paramagnetic-resonance spectroscopy and stopped-flow spectrophotometry on the mechanism of action of turkey liver xanthine dehydrogenase. | studies by e.p.r. (electron-paramagnetic-resonance) spectroscopy and by stopped-flow spectrophotometry on turkey liver xanthine dehydrogenase revealed strong similarities to as well as important differences from the veillonella alcalescens xanthine dehydrogenase and milk xanthine oxidase. the turkey enzyme is contaminated by up to three non-functional forms, giving molybdenum e.p.r. signals designated resting i, resting ii and slow. slow and to a lesser extent resting i signals are like those fr ... | 1976 | 179533 |
| atp formation associated with fumarate and nitrate reduction in growing cultures of veillonella alcalescens. | molar growth yields, fermentation balances and enzyme activities were measured in veillonella alcalescens grown anaerobically with different substrates in the absence or presence of fumarate or nitrate. the molar growth yields on malate (14.3 g dry wt bacteria/mole substrate) and citrate (19.3) were higher than that on lactate (8.6). the molar growth yield on lactate was increased to 15.5 or 19.8 by the addition of fumarate or nitrate, respectively, to the growth medium, and the molar growth yie ... | 1977 | 202192 |
| acetate kinase from veillonella alcalescens. purification and physical properties. | acetate kinase (atp:phosphotransferase e.c.2.7.2.1) has been purified to a high state of purity from veillonella alcalescens. the native enzyme had a molecular weight of 88,000, as determined by sephadex g-150 gel filtration. the molecular weight of the monomeric enzyme, estimated from sodium dodecyl sulfate-polyacrylamide gel electrophoresis, was 42,000. the enzyme was determined to be a homodimer from the amino acid composition and the results of trypsin digestion and cyanogen bromide cleavage ... | 1979 | 216674 |
| acetate kinase from veillonella alcalescens. regulation by succinate and substrates. | the kinetic properties of acetate kinase from veillonella alcalescens were investigated. in the presence of high concentrations of nucleotide both forward and reverse reactions were observed. in the presence of succinate the degree of cooperativity between subunits of the homodimer decreased, i.e. the hill coefficient, n, decreased from 2.5 to 1.4 for acetyl phosphate in the presence of succinate. at low substrate concentrations hyperbolic kinetic data were observed with succinate. we have propo ... | 1979 | 221506 |
| purification and regulatory properties of pyruvate kinase from veillonella parvula. | the nonglycolytic, anaerobic organism veillonella parvula m4 has been shown to contain an active pyruvate kinase. the enzyme was purified 126-fold and was shown by disc-gel electrophoresis to contain only two faint contaminating bands. the purified enzyme had a ph optimum of 7.0 in the forward direction and exhibited sigmoidal kinetics at varying concentrations o-f phosphoenol pyruvate (pep), adenosine 5'-monophosphate (amp), and mg-2+ ions with s0.5 values of 1.5, 2.0, and 2.4 mm, respectively. ... | 1975 | 238944 |
| symbiotic relationship of veillonella alcalescens and streptococcus mutans in dental plaque in gnotobiotic rats. | | 1978 | 272950 |
| [gas chromatography analysis of volatile monocarboxylic aliphatic acids (c2 to c6) of 15 strains of veillonella of oral origin]. | a qualitative and quantitative study has been carried out on 15 strains of the veillonella genus, isolated in the oral cavity. following two methods of preparation of samples for injection in a chromatograph gl, we have identified among the volatil monocarboxylic aliphatic acids from c2 to c6 the glucose fermentation products of these different strains. with the same methods, we studied in parallel these products from strains a.t.c.c. 10790-17444 (veillonella parvula) and 17445-17446 and 17447 ( ... | 1978 | 279541 |
| effect of intestinal bacteria on incidence of liver tumors in gnotobiotic c3h/he male mice. | the effect of intestinal microflora on liver tumorigenesis was studied in gnotobiotic c3h/he male mice monoassociated, diassociated, or polyassociated with the following strains of intestinal bacteria: escherichia coli, streptococcus faecalis, bifidobacterium adolescentis, bifidobacterium infantis, clostridium indolis, c. paraputrificum, c. perfringens, c. innocuum, c. nexile, c. ramosum, c. clostridiiforme, bacteroides multiacidus, bacteroides fragilis, veillonella alcalescens, v. parvula, and ... | 1979 | 292809 |
| [difference in nitrate reductase activity between veillonella alcalescens and veillonella parcula (author's transl)]. | | 1979 | 297483 |
| a reliable test for differentiation and presumptive identification of certain clinically significant anaerobes. | a modification of the slide catalase test was evaluated to determine its reliability for the presumptive identification of certain anaerobic gram-negative and gram-positive organisms isolated from clinical specimens. a total of 650 fresh isolates was evaluated. growth from a trypticase blood agar plate or schaedler's agar with laked blood was transferred to a slide and a drop of 15% h2o2 with tween 80 added. veillonella alcalescens, proprionibacterium species, peptococcus species, and bacteroide ... | 1978 | 339709 |
| a modified tube method for the cultivation and enumeration of anaerobic bacteria. | a new type of tube (the lee tube) has been developed for use in the cultivation and enumeration of obligate anaerobes. the lee tube is a double-walled, screw-capped tube which allows the formation of a thin cylinder of agar medium between the two walls. anaerobiosis is achieved through deoxygenation of the deep cylinder of agar during sterilization, a minimum of head space, and use of a reducing agent to absorb oxygen introduced during the inoculation procedure. for several species of clostridiu ... | 1979 | 396020 |
| bacteremia after upper gastrointestinal endoscopy. | during 24 months, 200 upper gastrointestinal endoscopies were performed on 193 patients. blood cultures were obtained before and five and 30 minutes after the procedure using thiol (50 ml) and trypticase soy broth (100 ml) media. the mean endoscopic time was 34 minutes. sixteen patients developed bacteremia (8%). twelve groups of microorganisms were detected in positive blood cultures: streptococcus (5 species), lactobacillus sp, veillonella alcalescens, staphylococcus aureus, staph epidermidis, ... | 1977 | 404974 |
| nitrite reduction in veillonella alcalescens. | nitrite reduction was examined in veillonella alcalescens c-1, and obligate anaerobe with an atp-yielding nitrate-reducing system. hydrogen donors for nitrite reduction included hydrosulfite, hydrogen gas, and pyruvate, but not pyridine nucleotides, in the presnece or absence of flavins. pyruvate-linked nitrite reduction was not inhibited by 4,4,4-trifluoro-1-(2-thienyl) 1,3-butanedione, dicoumarol, or 2-heptyl-4-hydroxy-quinoline-n-oxide. the noninvolvement of membrane-bound factors was support ... | 1979 | 422515 |
| in vitro activity of sodium fusidate against anaerobic bacteria. | the microtiter broth dilution method was employed to determine the in vitro susceptibility of 525 recent clinical isolates of anaerobic bacteria to sodium fusidate. the minimal inhibitory concentrations of sodium fusidate ranged from </=0.06 to 1.0 mug/ml for 155 strains of anaerobic gram-positive rods and 130 strains of anaerobic gram-positive cocci. minimal inhibitory concentrations ranging from </=0.06 to 32 mug/ml were observed for 240 strains of anaerobic gram-negative rods. among the latte ... | 1979 | 485124 |
| synthesis of alpha-ketoglutarate by reductive carboxylation of succinate in veillonella, selenomonas, and bacteriodes species. | evidence for reductive carboxylation of succinate to synthesize alpha-ketoglutarate was sought in anaerobic heterotrophs from the rumen and from other anaerobic habitats. cultures were grown in media containing unlabeled energy substrates plus [14c]succinate, and synthesis of cellular glutamate with a much higher specific activity than that of cellular asparate was taken as evidence for alpha-ketoglutarate synthase activity. our results indicate alpha-ketoglutarate synthase functions in selenomo ... | 1979 | 533772 |
| adherence of veillonella species mediated by extracellular glucosyltransferase from streptococcus salivarius. | the effect of extracellular products from streptococcus salivarius on sucrose-dependent adherence to smooth surfaces by other oral bacteria was studied in vitro. strains of streptococcus mitis, streptococcus pyogenes, and veillonella parvula without innate ability to adhere to a steel wire were able to do so when incubated with sucrose and cell-free culture fluid from s. salivarius strains 9759, 25975, cnii, and mepi. these culture fluids synthesized more adherent material and water-insoluble gl ... | 1977 | 591064 |
| interdependence of in vitro responsiveness of cord and maternal blood lymphocytes to antigens from oral bacteria. | a group of thirty-five mothers and their babies at parturition were examined by the in vitro lymphocyte transformation test to determine sensitization by oral bacterial antigens, b-cell mitogens and dental plaque. lymphocytes from babies of sensitized mothers with gingival or periodontal disease gave the highest frequency (70 and 63%) and magnitude (mean stimulation index of 3.4 and 3.3) of response in cultures stimulated by actinomyces viscosus and veillonella alcalescens. however, igm antibodi ... | 1977 | 606445 |
| veillonella parvula septicemia and osteomyelitis. | | 1977 | 835930 |
| affinity chromatography of antiserum to a gram negative organism. | an immunoadsorbent method is described for the purification of antibody directed against gram negative bacteria. two anion exchange materials were compared for their ability to immobilize whole cells of veillonella alcalescens, a gram negative oral bacterium. purified antibody preparations were applied to the columns and subsequently eluted with various combinations of desorbing buffers. the quantity of recovered antibody was measured and its activity assayed, using a microagglutination techniqu ... | 1977 | 864265 |
| nitrate-reductase electron-transport cofactors in veillonella alcalescens. | studies on the effects of inhibitors of the nitrate-reducing activity of veillonella alcalescens extracts suggest the participation of a naphthoquinone, a b-type cytochrome, and non-heme iron in electron transport to nitrate. a nitrate-reductase-deficient mutant displayed a longer doubling time and a decreased molar growth yield on nitrate media. this mutant was phenotypically restored by the addition of molybdate to the growth medium, giving evidence for the functioning of molybdenum in the nit ... | 1977 | 922606 |
| blastogenic response of human lymphocytes to oral bacterial antigens: characterization of bacterial sonicates. | soluble sonicate supernatant preparations were made from actinomyces viscosus (atcc 19246), a. naeslundii (atcc 12104), two strains of veillonella alcalescens (strain hv-1 and a human oral isolate), streptococcus sanguis (atcc 10556), s. mutans (strain 6715-t2), bacteroides melaninogenicus (strain k110), and leptotrichia buccalis (isolated from human dental plaque). these supernatants were characterized with reference to their chemical and antigenic components and their biological activity deter ... | 1976 | 977126 |
| establishment of defined microbial ecosystems in germ-free rats. ii. di-association of osborne-mendel rats with veillonella alcalescens and several oral microorganisms. | | 1976 | 1058741 |
| chemically defined media for growing anaerobic bacteria of the genus veillonella. | a chemically defined medium for veillonella parvula and v. alcalescens is described. some nutritional aspects of the two strains used were examined: the optimum concentration of reducing agents, the requirements for amino acids, diamines, vitamins and other growth factors, and the conditions needed for well balanced nutrition. no specific requirements for single amino acids were observed. a combination of l-cysteine, dl-aspartic acid, l-glutamic acid, l-serine and l-tyrosine, promoted growth. in ... | 1976 | 1087858 |
| symbiosis of streptococcus mutans and veillonella alcalescens in mixed continuous cultures. | | 1975 | 1096856 |
| characterization of a naturally occurring diamine auxotroph of veillonella alcalescens. | veillonella alcalescens strain atcc 17745 was shown to require putrescine or cadaverine for growth. none of the other compounds tried, including magnesium and spermidine, were able to substitute for the diamines. studies with labeled diamines showed that spermidine was made from putrescine in this organism. a polyamine analogous to spermidine, but made from cadaverine, was not found. a combination of growth experiments and chemical assays suggested that protein synthesis was limited in diamine-s ... | 1975 | 1194234 |
| lipid-phase transitions of the strictly anaerobic bacteria veillonella parvula and anaerovibrio lipolytica. | as a basis for physicochemical studies on the membranes of the strictly anaerobic bacteria veillonella parvula, anaerovibrio lipolytica, and megasphaera elsdenii, the fatty acyl and alk-1-enyl moieties on the phosphoglycerides of these organism were characterized. uncommon is the high proportion of a heptadecenoic acyl and alk-1-enyl moiety in these three lactate-fermenting bacteria. in contrast to v. parvula and a. lipolytica, m. elsdenii contains high amounts of branched-chain acyl and alk-1-e ... | 1975 | 1194242 |
| cell wall composition and incorporation of radio-labelled compounds by veillonella alcalescens. | the cell wall of veillonella alcalescens was shown to have a typically gram-negative appearance and composition. the wall contains 24% lipid, 0.8% phosphorus, and 6.8% hexosamine. it is estimated to contain about 5% murein, unlike the 24% reported by other for veillonella parvula. the amounts of 19 amino acids, including diaminopimelic acid, were determined. though veillonella sp. cannot metabolize sugars for energy, v. alcalescens incorporates ribose and fructose by separate, specific mechanism ... | 1975 | 1240789 |
| experimentally induced infection by oral anaerobic microorganisms in a mouse model. | a mouse model was developed to study the abscessogenic potential of pure and mixed cultures of oral anaerobes associated with infections of endodontic origin. maximum subcutaneous lesions were seen on their backs between 3 and 14 d after inoculation. strains of fusobacterium nucleatum, peptostreptococcus anaerobius, and veillonella parvula were pathogenic in pure culture. a mixed culture of f. nucleatum with either porphyromonas gingivalis or prevotella intermedia was significantly more pathogen ... | 1992 | 1408362 |
| complement activation by lipopolysaccharides purified from gram-negative bacteria isolated from infected root canals. | the purpose of the present study was to investigate the complement activation by lipopolysaccharides purified from porphyromonas (bacteroides) endodontalis, veillonella parvula, and fusobacterium nucleatum isolated from infected root canals. the rate of consumption of the c3t component of the complement increased remarkably when lipopolysaccharides of more than 10 micrograms were added. | 1992 | 1437068 |
| associations between microbial species in dental root canal infections. | the existence of commensal or antagonistic relationships between microorganisms in the root canals of teeth with apical periodontitis was investigated. samples were taken from 65 infected human root canals and were analysed according to species, frequency of occurrence and proportion of the total isolated flora. the most frequent species were fusobacterium nucleatum, prevotella intermedia, peptostreptococcus micros, peptostreptococcus anaerobius, eubacterium alactolyticum, eubacterium lentum and ... | 1992 | 1494447 |
| bacterial growth inhibition and short-term clinical efficacy of a vegetable oil-based mouthrinse: preliminary study. | bacterial growth of a vegetable oil-based product was determined in vitro. growth of streptococcus mutans and veillonella alcalescens was strongly inhibited for dilutions up to 50x. on the basis of these results, it was decided to evaluate the short-term, clinical efficacy of a 1:10 dilution of the concentrated product in water. fifteen human volunteers employed this rinse twice-a-day during a six-day period. planimetric plaque scores, as well as the plaque and gingival indexes according to löe, ... | 1992 | 1499249 |
| growth yield increase and atp formation linked to succinate decarboxylation in veillonella parvula. | veillonella parvula strain 259 (= dsm 2007) was able to grow on a mineral salts medium supplemented with (per litre) 1 g yeast extract, 1 g tween-80, and 3 mg putrescine.2 hcl, with 6 mm thioglycolate as reductant and lactate as growth substrate. succinate did not serve as a growth substrate, but when added in conjunction with lactate, it was decarboxylated to propionate and resulted in a measurable increase in growth yield, corresponding to the formation of 2.4 g cell dry mass per mol succinate ... | 1992 | 1510569 |
| osteomyelitis due to veillonella parvula: case report and review. | anaerobic gram-negative cocci of the genus veillonella are generally normal flora of the oropharynx and upper respiratory tract. when isolated in clinical specimens, these bacteria are usually regarded as commensal organisms rather than as pathogens. we report a case of spinal osteomyelitis in which veillonella parvula was isolated in pure culture from bone as well as blood. osteomyelitis of the spine due to anaerobic bacteria may occur more frequently than has been recognized, and anaerobic cul ... | 1992 | 1571454 |
| pyogenic sacroiliitis due to veillonella parvula. | | 1992 | 1617061 |
| modelling of mixed chemostat cultures of an aerobic bacterium, comamonas testosteroni, and an anaerobic bacterium, veillonella alcalescens: comparison with experimental data. | a mathematical model of mixed chemostat cultures of the obligately aerobic bacterium comamonas testosteroni and the anaerobic bacterium veillonella alcalescens grown under dual limitation of l-lactate and oxygen was constructed. the model was based on michaelis-menten-type kinetics for the consumption of substrates, with noncompetitive inhibition of v. alcalescens by o2. the growth characteristics of the aerobic and anaerobic organisms were determined experimentally with pure cultures of the ind ... | 1992 | 1622213 |
| energy conservation by succinate decarboxylation in veillonella parvula. | veillonella parvula cannot grow with succinate as sole energy source. however, succinate decarboxylation simultaneous with malate or lactate fermentation increased growth yields by 2.4-3.5 g (mol succinate)-1. malate was fermented stoichiometrically to acetate and propionate whereas lactate fermentation produced more acetate and considerable amounts of h2. aspartate was utilized only in the presence of succinate as co-substrate. methylmalonyl-coa decarboxylase and atp-dependent pyruvate carboxyl ... | 1992 | 1645132 |
| a comparison of human and animal mouth flora. | human bite injuries are often believed to be more serious than animal bite injuries. one reason for this distinction might be a species difference in the microbial mouth flora. in order to compare this possibility, we cultured the mouths of humans, dogs, and cats. human oral flora contained the smallest number of bacteria followed by dog and cat oral flora, respectively. veillonella parvula was cultured from all humans and was also found in dog and cat mouths, but less frequently. v parvula is n ... | 1991 | 1744782 |
| biological effects of veillonella parvula and bacteroides intermedius lipopolysaccharides. | a comparative study on the endotoxic effects of lipopolysaccharide (lps) from veillonella parvula atcc 10790 and from bacteroides intermedius bmh was performed using an in vivo approach in the c57bl/6 mouse. phenol-water extracted lps of such anaerobes was purified by ultracentrifugation and dnase/rnase digestion, and characterized by a metachromatic assay for endotoxins and by electrophoresis on sds-polyacrylamide gel and silver staining. mouse ld50 for v. parvula lps was 1.479 mg and for b. in ... | 1991 | 1775087 |
| [infective endocarditis and pulmonary abscess in an intravenous drug addict caused by veillonella parvula]. | | 1991 | 1784772 |
| comparative in vitro activity of cefpodoxime against anaerobes other than bacteroides fragilis. | to assess the in vitro activity of cefpodoxime against anaerobic respiratory tract and oropharyngeal pathogens 77 strains belonging to 18 gram-negative and 7 gram-positive species were studied by means of agar dilution tests. for comparison cefuroxime, amoxicillin, amoxicillin + clavulanic acid and clindamycin were also tested. cefpodoxime was found to be active at concentrations of less than or equal to 0.125 mg/l against prevotella oralis, prevotella buccalis, prevotella bivia, porphyromonas a ... | 1991 | 1800380 |
| [anaerobic bacteria and their role in recurrent tonsillitis]. | the presence of anaerobic bacteria in 100 palatal tonsils obtained during the tonsillectomy, was evaluated in quality and in quantity. 17 species of anaerobic bacteria were found. the most frequent were: bacteroides melaninogenicus, peptostreptococcus anaerobius, peptostreptococcus intermedius, propionibacterium, veillonella parvula and peptococcus asaccharolyticus. in most evaluations their density was 10(5)-10(6) in gram of tonsil tissue, sometimes 10(7). its role in recurrent tonsils infectio ... | 1990 | 1981606 |
| t cell proliferative responses to molecular fractions of periodontopathic bacteria. | soluble antigenic preparations of veillonella parvula and bacteroides gingivalis were separated by sds-page and used after electroblotting and solubilization for in vitro lymphocyte stimulation in 13 patients with severe periodontitis and 12 controls. the cellular responses of controls and patients to v. parvula antigens were represented by four main proliferation-inducing fractions with 74-66, 52-46, 22-19 and 12 kd mol. wt. these fractions induced slightly enhanced dna synthesis in lymphocytes ... | 1991 | 1988218 |
| on the mechanism of sodium ion translocation by methylmalonyl-coa decarboxylase from veillonella alcalescens. | veillonella alcalescens during lactate degradation developed an na+ concentration gradient with 7-8 times higher external than internal na+ concentrations in the logarithmic growth phase. the gradient declined to a factor of 1.9 in the late stationary phase. methylmalonyl-coa decarboxylase reconstituted into proteoliposomes performed an active electrogenic na+ transport, creating delta psi of 60 mv, delta pna+ of 50 mv, and delta mu na+ of 110 mv. in the initial phase of the transport, the decar ... | 1991 | 1991479 |
| [immunobiological activities of veillonella parvula isolated from infected root canals]. | the primary etiological agent of all periapical lesions has long been considered to be bacterial. furthermore, bacteria from infected root canals are potential antigens capable of initiating immunological reactions in periapical tissues. the purpose of this study, therefore, was clarify the immunological potentials of veillonella parvula (v. parvula), which was frequently isolated from root canals with periapical lesion. immunobiological activities of v. parvula sonic extracts were investigated ... | 1990 | 2134271 |
| characterization of the tet m determinants in urogenital and respiratory bacteria. | tetracycline-resistant fusobacterium nucleatum, haemophilus ducreyi, mycoplasma hominis, peptostreptococcus spp., ureaplasma urealyticum, and veillonella parvula had dna sequences which showed homology throughout the length of the tet m transposon, tn916. in contrast, gardnerella vaginalis, commensal neisseria spp., and the 25.2-megadalton plasmid family lacked the complete transposon. | 1990 | 2159259 |
| recolonization of the subgingival microflora after scaling and root planing in human periodontitis. | the purpose of this study was to evaluate the recolonization patterns of the subgingival microflora of adult periodontitis patients after a single session of scaling and root planing. in each of eight patients, three clinically diseased sites were investigated microbiologically by darkfield microscopy and cultural analysis. after initial clinical and microbiological parameters were determined, each subject received a single session of scaling and root planing but no oral hygiene instructions. cl ... | 1990 | 2213468 |
| growth stimulation of treponema denticola by periodontal microorganisms. | previous experiments have indicated that enrichment of subgingival plaque in human serum can lead to the accumulation of treponema denticola. t. denticola depends on bacterial interactions for its growth in serum. aim of the present study was to identify specific microorganisms involved in the growth stimulation of t. denticola. to this end, strains isolated from previous plaque enrichment cultures were tested for growth stimulation in co-cultures with t. denticola. in addition, growth of t. den ... | 1990 | 2321929 |
| susceptibility and resistance of plaque bacteria to minocycline. | the in vitro susceptibility of 55 strains of subgingival plaque bacteria to minocycline was determined. a concentration of 1 microgram/ml minocycline was found to inhibit 85% of the strains tested and the mic ranged from 0.03 to 32 micrograms/ml. for 71% of the strains tested the mbc was at least 4 times greater than the corresponding mic, suggesting a bacteriostatic activity for minocycline. a concentration of 20 mg/ml of magnesium ions was capable of neutralizing 8 micrograms/ml of minocycline ... | 1990 | 2324922 |
| surface-associated properties of streptococcus milleri group strains and their potential relation to pathogenesis. | thirty strains from the streptococcus milleri (anginosus) group (smg) obtained from various sources were tested for a range of characters that could be associated with pathogenicity and the results were compared with those for type strains of s. sanguis, s. mutans and s. pyogenes. the smg strains were heterogeneous in all tests. most (18) belonged to one of the lancefield groups with group f predominating. adherence of strains isolated from abscesses to buccal epithelial cells was greater than t ... | 1990 | 2325115 |
| genetic basis of tetracycline resistance in urogenital bacteria. | the distributions of the nucleotide sequences related to the tetracycline resistance determinants tet k, tet l, tet m, and tet o were studied by dot blot hybridization with randomly chosen clinical urogenital tract isolates of viridans group streptococci, streptococcus agalactiae, enterococcus faecalis, gardnerella vaginalis, lactobacillus spp., fusobacterium nucleatum, peptostreptococcus spp., and veillonella parvula. among the peptostreptococcus spp., 79% of the isolates hybridized with one (6 ... | 1990 | 2327774 |
| microflora associated with experimental root surface caries in humans. | this study describes the microflora from actively progressing root surface caries lesions, in which mineral loss had been determined by quantitative microradiography. the caries lesions were produced experimentally in root surface specimens from human molars inserted in lower partial dentures carried for 3 months by six elderly individuals. a total of 780 bacterial isolates were identified from 13 plaque samples, collected with a punch technique, and six dentin samples. the composition of the mi ... | 1990 | 2341169 |
| ruthenium red staining reveals surface fibrils and a layer external to the cell wall in streptococcus salivarius hb and adhesion deficient mutants. | ruthenium red staining revealed both the long and short classes of cell surface fibril in thin sections of streptococcus salivarius hb, indicating that the fibrils contained polyanionic polymers, probably polysaccharides. also visible was a 16.2 +/- 2.2 nm thick ruthenium red staining layer (rrl) outside the 16.7 +/- 2.2 nm thick cell wall. the fibrils could not be seen after conventional glutaraldehyde and osmium fixation. the rrl was protease resistant and was not involved in septum formation. ... | 1988 | 2479709 |
| bacterial meningitis caused by veillonella parvula. | a three-year-old girl injured her right eyelid with a toothbrush. the wound was sutured. swelling of the eyelid, high fever and vomiting developed in spite of oral antibiotics for seven days. the findings of the cerebrospinal fluid (csf) were white blood cells (wbc) 26,368/mm3 (90% polymorphs), protein 127 mg/dl, and sugar 0 mg/dl. although gram negative organisms were seen on the smear, aerobic culture was sterile. later culture of csf on admission grew anaerobic bacteria: veillonella parvula. ... | 1989 | 2515742 |
| metabolism of gentiopicroside (gentiopicrin) by human intestinal bacteria. | as a part of our studies on the metabolism of crude drug components by intestinal bacteria, gentiopicroside (a secoiridoid glucoside isolated from gentiana lutea), was anaerobically incubated with various defined strains of human intestinal bacteria. many species had ability to transform it to a series of metabolites. among them, veillonella parvula ss parvula produced five metabolites, which were identified as erythrocentaurin, gentiopicral, 5-hydroxymethylisochroman-1-one,5-hydroxymethylisochr ... | 1989 | 2605687 |
| the predominant cultivable microbiota of crevicular epithelial cells. | the purpose of the present investigation was to compare the composition of the predominant cultivable microbiota associated with gingival crevicular epithelial cells with that of the unattached microbiota recovered from the same site. samples were taken from 2 diseased sites from 8 periodontal patients, by scraping the epithelial lining of the pocket with a curette. the epithelial cells were separated from the unattached subgingival bacteria by centrifugation in a reduced 50% percoll density gra ... | 1989 | 2628861 |
| expression of the surface properties of the fibrillar streptococcus salivarius hb and its adhesion deficient mutants grown in continuous culture under glucose limitation. | streptococcus salivarius hb and four adhesion deficient mutants, hb-7, hb-v5, hb-v51 and hb-b, were grown in continuous culture in a defined medium under glucose limitation over a range of growth rates from 0.1 to 1.1 h-1. the ability to coaggregate with veillonella parvula v1 cells and the ability to adhere to buccal epithelial cells did not alter with increasing growth rate. cell surface hydrophobicity decreased markedly with increasing growth rate for the non-fibrillar non-adhesive mutant hb- ... | 1989 | 2632665 |
| [comparative study of the antimicrobial effect of various cavity liners used in conservative dentistry]. | we have compared the microbiological activity of the following cavity liners: life, dycal ii, calcipulpe, pure calcium hydroxide and cavitec; against five different bacterial strains: veillonella parvula, bacteroides fragilis, peptococcus s.p., staphylococcus aureus, and streptococcus beta hemolytic: the results demonstrate the higher antimicrobial activity of the manufactured cavity liners with calcium hydroxide base in comparison with the pure calcium hydroxide. | 1989 | 2638022 |
| detection of immunodominant antigens of periodontopathic bacteria in human periodontal disease. | sonicated whole cell extracts and outer membrane proteins (omp) from bacteroides gingivalis and veillonella parvula were analysed by the immunoblot technique using sera from 103 patients with various forms of periodontal disease and from 31 control subjects. b. gingivalis sonicate contained 12 major bands (75-14 kda) of which the 46, 27 and 14 kda antigens reacted more frequently with sera from adult and young adult patients with severe periodontitis compared with sera from controls and mild per ... | 1989 | 2639300 |
| cytotoxicity against various cell lines of lipopolysaccharides purified from bacteroides, fusobacterium, and veillonella isolated from infected root canals. | the cytotoxicity against two mesenchymal cell lines, l-929 and wi-38, and two epithelial cell lines, kb and hela s-3, of lipopolysaccharide (lps) purified from strains of bacteroides gingivalis, fusobacterium nucleatum, and veillonella parvula isolated from infected root canals was investigated. the inhibition of cell growth by these lps's was considerable for mesenchymal cell lines, but mild for epithelial cell lines. the cytotoxic effect of f. nucleatum lps was the greatest and that of b. ging ... | 1989 | 2639950 |
| subgingival microflora, dental and periodontal conditions in patients with hereditary fructose intolerance. | hereditary fructose intolerance (hfi) is a rare autosomally recessive disease which leads to severe hypoglycemia. the fructose-free diet of these patients apparently influences dental health. half of the patients are free of caries, but there are no reports on their periodontal condition nor on the composition of their subgingival microflora. therefore 18 patient with hfi were examined for the following parameters: radiographic bone loss, caries rate, gingival bleeding, occurrence of calculus, p ... | 1989 | 2704977 |
| a cervicofacial infection due to veillonella parvula in a patient with myxoedema. | | 1989 | 2713184 |
| 'a cervicofacial infection due to veillonella parvula in a patient with myxoedema'. | | 1989 | 2751904 |
| bacterial flora in chronic tonsillitis. | a study of aerobic and anaerobic bacterial flora in chronic tonsillitis revealed a total of 59 isolates. aerobic flora from the surface swab was found in 80%, from the core of the tonsil in 92% and from both the surface and the core in 68% of the cases. there were 30 aerobic isolates from the surface and 29 isolates from the core. mixed flora were present from the surface and the core in 10 and 7 cases, respectively. pathogens were seen in 36% of the cases from the surface and in 40% from the co ... | 1989 | 2759695 |
| bilateral abscessed orchiepididymitis associated with sepsis caused by veillonella parvula and clostridium perfringens: case report and review of the literature. | veillonella species is a gram-negative coccus which is part of the anaerobic normal flora in the oral cavity, small intestine, upper respiratory tract, vagina, and urinary tract. the role that this organism plays in infection is not well known, and it is generally associated with other bacteria. we present a case of bilateral abscessed orchiepididymitis associated with septicemia due to veillonella parvula and, later, to clostridium perfringens, with the development of severe renal insufficiency ... | 1987 | 2887584 |
| the carboxyltransferase activity of the sodium-ion-translocating methylmalonyl-coa decarboxylase of veillonella alcalescens. | methylmalonyl-coa decarboxylase of veillonella alcalescens catalyzed the isotopic exchange between methylmalonyl-coa and [1-14c]propionyl-coa or between malonyl-coa and [1-14c]acetyl-coa. the exchange was independent of sodium ions and was abolished by avidin. the enzyme also catalyzed the carboxyl transfer reaction from methylmalonyl-coa to acetyl-coa yielding propionyl-coa and malonyl-coa, and vice versa. the beta subunit was dissociated from methylmalonyl-coa decarboxylase by prolonged washin ... | 1989 | 2920730 |
| the effect of lowering the ph on the composition and metabolism of a community of nine oral bacteria grown in a chemostat. | nine oral bacteria, associated with both healthy and diseased sites in the mouth, were grown at d = 0.05 h-1 (mean generation time 13.9 h) in a glucose-limited chemostat. after an initial period of steady-state growth at ph 7.0, ph control was discontinued. the ph then decreased until it stabilized at ph 4.1 after 9 d (16 generations), while the eh rose from -165 mv to +160 mv. the lowering in ph resulted in the composition and metabolism of the flora being altered and in increased bacterial agg ... | 1986 | 3095488 |
| nitrite accumulation during anaerobic nitrate reduction by binary suspensions of bacteria isolated from the achlorhydric stomach. | binary suspensions of bacteria isolated from the gastric juice of achlorhydric patients were used to determine conditions which favour nitrite accumulation during nitrate reduction. suspensions of veillonella parvula and haemophilus parainfluenzae accumulated nitrite during nitrate reduction in the absence of nitrite-reducing neisseria subflava or streptococcus sanguis. the maximum concentration of nitrite that transiently accumulated decreased predictably as the ratio of nitrite-removing bacter ... | 1987 | 3117970 |
| production of volatile fatty acids as a result of bacterial interactions in the cecum of gnotobiotic rats and chickens fed a lactose-containing diet. | volatile fatty acid (vfa) productions from lactose and lactate by a clostridium butyricum and a veillonella alcalescens strain, alone or in combination with a lactobacillus acidophilus strain, were determined both in vitro in culture media and in vivo in the ceca of gnotobiotic animals. gnotobiotic rats, which possess intestinal lactase, and chickens, which are devoid of it, were used. both animal species were fed a diet containing 4% lactose. the in vitro results showed that the c. butyricum st ... | 1988 | 3148988 |
| bacterial involvement in denture-induced stomatitis. | denture and mucosal plaque samples were collected from eight full-denture wearers of whom four suffered from denture-induced stomatitis (dis). cultures were made, and a proportional identification to species level was carried out of bacteria and yeasts. an inventory was made of the predominant flora. results showed that the predominant microflora of both groups, both on dentures and the denture-bearing mucosa, consisted mainly of gram-positive bacteria. differences in the proportions of cocci we ... | 1988 | 3166009 |
| coaggregation properties of human oral veillonella spp.: relationship to colonization site and oral ecology. | the primary habitats of oral veillonellae are the tongue, dental plaque, and the buccal mucosa. isolates were obtained from each habitat and tested for coaggregation with a battery of other oral bacterial strains. all 59 tongue isolates tested for coaggregation were veillonella atypica or veillonella dispar. all but one of them coaggregated with strains of streptococcus salivarius, a predominant inhabitant of the tongue surface but not subgingival dental plaque. these tongue isolates were unable ... | 1988 | 3178207 |
| tetracycline resistance and tetm in oral anaerobic bacteria and neisseria perflava-n. sicca. | tetracycline-resistant organisms isolated from six patients with periodontal disease included bacteroides spp., eubacterium spp., fusobacterium nucleatum, neisseria perflava-n. sicca, peptostreptococcus anaerobius, veillonella parvula, and facultative streptococci. all but the bacteroides spp. and eubacterium spp. hybridized with the tetm determinant. an additional 417 bacterial strains were screened, and 4% of both the oral streptococci and the fusobacterium spp. hybridized with the tetm probe. | 1988 | 3190205 |
| monobacterial and mixed bacterial plaques of streptococcus mutans and veillonella alcalescens in an artificial mouth: development, metabolism, and effect on human dental enamel. | to gain greater understanding of the role of streptococcus mutans and veillonella in the caries process, studies of both aerobically and anaerobically grown plaques of s. mutans c67-1 and v. alcalescens v-1 on human enamel slabs were carried out in an artificial mouth. plaque development, acid production, and demineralization were measured. early plaque development of monobacterial and mixed bacterial plaques started from randomly adhering cells on day 1 to confluent multilayered microcolonies o ... | 1988 | 3214847 |
| microbial flora of surface versus core tonsillar cultures in recurrent tonsillitis in children. | surface and 'core' tonsillar specimens were collected from 60 children, between 2 and 14 years of age, suffering from recurrent tonsillitis, in order to establish the reliability of surface tonsillar swabbing technique and to define the most frequently involved aerobic and anaerobic microorganisms. from the qualitative point of view, the same mixed aerobic and anaerobic flora were obtained in both samples thus demonstrating the reliability of the surface swabbing technique. from the quantitative ... | 1988 | 3397235 |
| interactions between rumen amylolytic and lactate-utilizing bacteria in growth on starch. | the growth and metabolism of the rumen amylolytic bacteria streptococcus bovis, butyrivibrio fibrisolvens and bacteroides ruminicola, growing in pure cultures and co-cultures with the rumen lactilytic bacteria megasphaera elsdenii and veillonella alcalescens were followed. the interaction of amylolytic bacteria with v. alcalescens represents a simple food chain. the interaction with m. elsdenii is more complex, since there is a simultaneous competition for products of the starch degradation. | 1987 | 3429358 |
| [indirect immunohistochemical test of the endotoxin of veillonella alcalescens and of peptidoglycans in the root cementum of periodontally diseased teeth]. | | 1986 | 3461943 |
| serum igg antibodies to lipopolysaccharides in various forms of periodontal disease in man. | serum igg antibody titres to lipopolysaccharides (lps) from two strains of actinobacillus actinomycetemcomitans were significantly elevated in juvenile periodontitis compared with other types of periodontal disease and with controls (p less than 0.05). the highest antibody titres to bacteroides gingivalis lps were in juvenile periodontitis, but this difference was significant only against the control group (p less than 0.01). in adult mild periodontitis there were higher antibody levels to lps f ... | 1986 | 3479058 |
| putrescine and cadaverine are constituents of peptidoglycan in veillonella alcalescens and veillonella parvula. | veillonella alcalescens atcc 17745, a strictly anaerobic, gram-negative small coccus, requires putrescine or cadaverine for growth (m. b. ritchey, and e. a. delwiche, j. bacteriol. 124:1213-1219, 1975). both putrescine and cadaverine were demonstrated to be incorporated exclusively into the peptidoglycan layer of v. alcalescens atcc 17745. v. parvula gai 0574 also proved to contain putrescine as a component of peptidoglycan. the primary chemical structure of the peptidoglycan common to the two v ... | 1987 | 3584075 |
| stereochemistry of the methylmalonyl-coa decarboxylation reaction. | the steric course of the decarboxylation of (s)-methylmalonyl-coa to propionyl-coa, catalyzed by the biotin-dependent sodium pump methylmalonyl-coa decarboxylase of veillonella alcalescens was determined. the decarboxylation of (s)-methylmalonyl-coa in 3h2o yielded (r)-[2-3h]propionyl-coa; and the decarboxylation of (s)-[2-3h]methylmalonyl-coa in h2o produced (s)-[2-3h]propionyl-coa. the results demonstrate retention of configuration during the decarboxylation reaction. the substrate stereochemi ... | 1987 | 3609308 |
| structural specificity of diamines covalently linked to peptidoglycan for cell growth of veillonella alcalescens and selenomonas ruminantium. | putrescine and cadaverine are essential constituents of the peptidoglycan of veillonella alcalescens, veillonella parvula, and selenomonas ruminantium and are necessary for the growth of these organisms (y. kamio and k. nakamura, j. bacteriol. 169:2881-2884, 1987, and y. kamio, h. pösö, y. terawaki, and l. paulin, j. biol. chem. 261:6585-6589, 1986). in this study, the structural specificity of the diamine requirement for normal cell growth of these bacteria was examined by using a series of dia ... | 1987 | 3654585 |
| identification of anaerobic bacteria using high-performance liquid chromatography. | 20 species of the genera clostridium, bacteroides, fusobacterium and the type strains of peptostreptococcus anaerobius, peptococcus asaccharolyticus, veillonella parvula and propionibacterium acnes were examined for the production of volatile (vfa) and nonvolatile (nvfa) short-chain fatty acids using high-performance liquid chromatography (hplc) with a column for organic acids (aminex hpx-87h). 10 min are needed for sample preparation and the vfa and nvfa were detected simultaneously in a single ... | 1987 | 3673341 |
| activation of phosphoenolpyruvate carboxykinase isolated from veillonella parvula. | phosphoenolpyruvate carboxykinase (pepck, ec 4.1.1.49) has been purified 940-fold from veillonella parvula using protamine sulphate treatment, ammonium sulphate precipitation, and column chromatography. the purified enzyme was substantially free of contaminating enzymes or proteins. maximum activity in the direction of oxaloacetate (oaa) decarboxylation was exhibited at ph 9.0. at this ph, the v. parvula enzyme catalysed phosphoenolpyruvate formation in the presence of mn2+ ions. in the presence ... | 1986 | 3778663 |
| relationship of cell surface morphology and composition of streptococcus salivarius k+ to adherence and hydrophobicity. | the cell surfaces of a range of variants of streptococcus salivarius hb, altered in cell wall antigen composition, were compared with those of the parent with respect to adherence, ability to adsorb to hexadecane, morphology, and exposure of lipoteichoic acid (lta). adherence to host surfaces was measured by using both saliva-coated hydroxyapatite beads and tissue-cultured hela cells, and interbacterial adherence was measured by using veillonella alcalescens v1 cells. progressive loss of the pro ... | 1987 | 3804445 |
| [role of nitrates in lactate metabolism in the oral bacterium, veillonella parvula]. | | 1985 | 3869047 |
| a new apparatus for continuous cultivation of bacterial plaque on solid surfaces and human dental enamel. | a new apparatus for the continuous cultivation of mono and mixed bacterial plaque on solid surfaces is described. the features are: easy preparation and handling; freedom from technical problems and microbial contamination; self-sufficient for periods of up to 56 d; 12 samples are taken simultaneously; programmable supply inlet. experiments were performed with streptococcus mutans c 67-1 for mono bacterial inoculation and in combination with veillonella alcalescens v-1 for mixed bacterial inocul ... | 1985 | 3897164 |
| dental pulp response to bacterial cell wall material. | lipopolysaccharides (lps) from bacteroides oralis and veillonella parvula and cell wall material from lactobacillus casei were studied for their capacity to induce leukocyte migration in the dental pulp and in an implanted wound chamber. three adult monkeys were challenged using lyophilized material sealed into buccal class v cavities prepared in dentin. pulp tissue responses were observed histologically eight and 72 hours after initiation of the experiment. subjacent to cut dentinal tubules, ba ... | 1985 | 3926844 |
| use of parenteral antibiotic therapy to eradicate bacterial colonization of amniotic fluid in premature rupture of membranes. | the successful eradication of bacterial colonization of the amniotic fluid with parenteral maternal antibiotic therapy in a preterm gestation with premature rupture of membranes is reported. amniotic fluid, obtained by amniocentesis, of a pregnancy complicated by premature rupture of membranes at 29 weeks was found to have three different bacterial species (bacteroides bivius, veillonella parvula, and peptococcus). because of prematurity and clinical quiescence, parenteral antibiotics were admin ... | 1986 | 3945461 |
| nitrate reduction and the growth of veillonella alcalescens. | veillonella alcalescens, a strict anaerobe, was found to possess a nitrate reductase system which has characteristics of both assimilatory and respiratory nitrate reduction. the nitrate reductase has been identified tentatively as a particulate enzyme which utilizes a variety of electron donors for the reduction of nitrate. by use of (15)n-labeled nitrate, it was shown that under appropriate conditions nitrate nitrogen is incorporated into cell material. v. alcalescens grown on pyruvate and nitr ... | 1973 | 4145863 |
| interactions of the complement system with the surface and endotoxic lipopolysaccharide of veillonella alcalescens. | electron microscopic studies demonstrated that lesions were produced on the endotoxic lipopolysaccharide (lps) as well as on the cell surface of v. alcalescens after reaction with fresh guinea pig serum. these lesions were approximately 90 a in diameter, and were seen on two characteristic structural entities derived from lps preparations after incubation with serum. the use of numerous inhibitors, inactivators, and reaction conditions affecting hemolytic c' activity revealed that these lesions ... | 1967 | 4164691 |
| interactions of the complement system with native and chemically modified endotoxins. | endotoxic lipopolysaccharides (lps) isolated from serratia marcescens, veillonella alcalescens, and salmonella typhosa were potent in their ability to induce fixation of complement (c') in normal guinea pig, rabbit, mouse, and human serum. the c'-fixing ability of lps was pronounced even when assays were performed in undiluted serum, and was lost after each of four chemical modifications which resulted in loss of biological toxicities. the detoxification procedures had in common the cleavage of ... | 1968 | 4170751 |
| the isolation and characterization of malate-lactate transhydrogenase from micrococcus lactilyticus. | | 1966 | 4289051 |
| lactate metabolism by veillonella parvula. | a strain of veillonella parvula m4, which grows readily in lactate broth without a requirement for carbon dioxide, has been isolated from the oral cavity. anaerobic, washed cells of this organism fermented sodium lactate to the following products (moles/100 moles of lactate): propionate, 66; acetate, 40; carbon dioxide, 40; and hydrogen, 14. cells grew readily in tryptone-yeast extract broth with pyruvate, oxaloacetate, malate, and fumarate, but poorly with succinate. the fermentation of pyruvat ... | 1971 | 4323300 |