| [nitrofurantoin-test for the differentiation of bordetella bronchiseptica (author's transl)]. | bordetella bronchiseptica is primarily resistant against nitrofurantoin (mic greater than 200 mug/ml), and this feature can be used for the differentiation of the organism from other gram-negative coccobacteria. nitrofurantoin paper disks (300 mug) failed to affect the growth of 150 strains of b. bronchiseptica isolated from different animal hosts, but they produced marked inhibition zones in the cultures of the followingspecies: pasteurella multocida, pasteurella pneumotropica, pasteurella haem ... | 1975 | 809944 |
| isolation and partial characterization of a phage receptor from "brucella neotomae" 5k33. | | 1977 | 907381 |
| the taxonomic properties of brucellae isolated from neotoma lepida thomas (brucella neotomae stoenner). | | 1967 | 4971180 |
| binding of bacteria from the genus brucella to human b lymphocytes. | in previous studies, we have shown that various lymphocyte subpopulations bind different strains of bacteria of different genera and species. among these bacteria was a strain of brucella melitensis which bound to all human b lymphocytes. to determine whether the binding of b. melitensis to human b lymphocytes was strain, species, or genus characteristic, we tested the binding of b. melitensis, brucella abortus, brucella ovis, brucella suis, brucella canis and brucella neotomae to human normal a ... | 1981 | 6783546 |
| nucleotide sequence and expression of the gene encoding the major 25-kilodalton outer membrane protein of brucella ovis: evidence for antigenic shift, compared with other brucella species, due to a deletion in the gene. | the nucleotide sequences encoding the major 25-kda outer membrane protein (omp) (omp25 genes) of brucella ovis 63/290, brucella melitensis 16m, brucella suis 1330, brucella canis rm6/66, and brucella neotomae 5k33 (all reference strains) were determined and compared with that of brucella abortus 544 (p. de wergifosse, p. lintermans, j. n. limet, and a. cloeckaert, j. bacteriol. 177:1911-1914, 1995). the major difference found was between the omp25 gene of b. ovis and those of the other brucella ... | 1996 | 8675306 |
| dna polymorphism at the omp-31 locus of brucella spp.: evidence for a large deletion in brucella abortus, and other species-specific markers. | the omp-31 gene, encoding a major outer-membrane protein in brucella melitensis, was pcr-amplified from brucella strains representing all species and known biovars by using primers selected according to the b. melitensis 16m omp-31 published sequence. amplification of omp-31 was achieved from dna of all brucella species with the exception of brucella abortus, the only brucella species where expression of omp-31 was not detected by reactivity with an mab specific for an epitope located in omp-31. ... | 1997 | 9308175 |
| limited genetic diversity of brucella spp. | multilocus enzyme electrophoresis (mlee) of 99 brucella isolates, including the type strains from all recognized species, revealed a very limited genetic diversity and supports the proposal of a monospecific genus. in mlee-derived dendrograms, brucella abortus and a marine brucella sp. grouped into a single electrophoretic type related to brucella neotomae and brucella ovis. brucella suis and brucella canis formed another cluster linked to brucella melitensis and related to rhizobium tropici. th ... | 2001 | 11136777 |
| metabolic studies on brucella neotomae (stoenner and lackman). | | 1958 | 13598716 |
| experimental infection of swine with brucella neotomae. | | 1959 | 13797868 |
| the behavior of brucella neotomae and brucella suis in reciprocal superinfection experiments in mice and guinea pigs. | | 1963 | 13984251 |
| host-parasite relationships with brucella neotomae. | gibby, irvin w. (cornell university medical college, new york, n.y.), and anna m. gibby. host-parasite relationships with brucella neotomae. j. bacteriol. 89:9-16. 1965.-an investigation was undertaken to ascertain whether a vigorous parasitic state could be evolved by transfer procedures in the host-parasite system, brucella neotomae versus the white mouse. visible disease was not maintained and initial high doses were abruptly decreased if the parasite was serially transferred through host ani ... | 1965 | 14255687 |
| identification of species of brucella using fourier transform infrared spectroscopy. | fourier transform infrared spectroscopy (ftir) is a technique that has been used over the years in chemical analysis for the identification of substances and is one that may be applied to the characterisation of microorganisms. the marked tendency of brucella towards variation in the smooth rough phase, together with the laboriousness and risk involved in the methods used in their identification, make their classification difficult. we studied the type strains of the different species and biovar ... | 2003 | 14500003 |
| brucella inopinata sp. nov., isolated from a breast implant infection. | a gram-negative, non-motile, non-spore-forming coccoid bacterium (strain bo1(t)) was isolated recently from a breast implant infection of a 71-year-old female patient with clinical signs of brucellosis. affiliation of strain bo1(t) to the genus brucella was confirmed by means of polyamine pattern, polar lipid profile, fatty acid profile, quinone system, dna-dna hybridization studies and by insertion sequence 711 (is711)-specific pcr. strain bo1(t) harboured four to five copies of the brucella-sp ... | 2010 | 19661515 |
| immunization of mice with gamma-irradiated brucella neotomae and its recombinant strains induces protection against virulent b. abortus, b. melitensis, and b. suis challenge. | human brucellosis, a zoonotic disease of major public health concern in several developing countries, is primarily caused by brucella abortus, brucella melitensis, and brucella suis. no brucellosis vaccine is available for human use. the aim of this study was to determine if brucella neotomae, a bacterium not known to cause disease in any host, can be used for developing brucellosis vaccines. b. neotomae and its recombinant strains overexpressing superoxide dismutase and a 26 kda periplasmic pro ... | 2010 | 21109033 |
| identification of a single-nucleotide insertion in the promoter region affecting the sodc promoter activity in brucella neotomae. | brucella neotomae is not known to be associated with clinical disease in any host species. previous research suggested that b. neotomae might not express detectable levels of cu/zn superoxide dismutase (sod), a periplasmic enzyme known to be involved in protecting brucella from oxidative bactericidal effects of host phagocytes. this study was undertaken to investigate the genetic basis for the disparity in sod expression in b. neotomae. our western blot and sod enzyme assay analyses indicated th ... | 2010 | 21124845 |
| comparison of six commercial dna extraction kits for detection of brucella neotomae in mexican and central american-style cheese and other milk products. | raw or inadequately pasteurized milk from infected animals and cheese made with such milk are a frequent vehicle for human brucellosis infection. also, biological terrorism is a concern with certain brucella spp. due to matrix-associated real-time polymerase chain reaction (qpcr) inhibitors, robust sample preparations are crucial. we compared six commercial nucleic acid extraction kits using nine mexican and central american-style soft cheeses or creams and three liquid milk products inoculated ... | 2012 | 23498184 |
| oral immunization of mice with gamma-irradiated brucella neotomae induces protection against intraperitoneal and intranasal challenge with virulent b. abortus 2308. | brucella spp. are gram-negative, facultative intracellular coccobacilli that cause one of the most frequently encountered zoonosis worldwide. humans naturally acquire infection through consumption of contaminated dairy and meat products and through direct exposure to aborted animal tissues and fluids. no vaccine against brucellosis is available for use in humans. in this study, we tested the ability of orally inoculated gamma-irradiated b. neotomae and b. abortus rb51 in a prime-boost immunizati ... | 2014 | 25225910 |
| advancement of knowledge of brucella over the past 50 years. | fifty years ago, bacteria in the genus brucella were known to cause infertility and reproductive losses. at that time, the genus was considered to contain only 3 species: brucella abortus, brucella melitensis, and brucella suis. since the early 1960s, at least 7 new species have been identified as belonging to the brucella genus (brucella canis, brucella ceti, brucella inopinata, brucella microti, brucella neotomae, brucella ovis, and brucella pinnipedialis) with several additional new species u ... | 2014 | 24981716 |
| a repa-based elisa for discriminating cattle vaccinated with brucella suis 2 from those naturally infected with brucella abortus and brucella melitensis. | the commonest ways of diagnosing brucellosis in animals include the rose-bengal plate agglutination test, the buffered plate agglutination test (bpa), the slide agglutination test, the complement fixation test, and the indirect enzyme linked immunosorbent assay (i-elisa). however, these methods cannot discriminate the brucella vaccine strain (brucella suis strain 2; b. suis s2) from naturally acquired virulent strains. of the six common brucella species, brucella melitensis, brucella abortus, an ... | 2014 | 24941369 |
| comparative whole genome analysis of six diagnostic brucellaphages. | whole genome sequencing of six diagnostic brucellaphages, tbilisi (tb), firenze (fz), weybridge (wb), s708, berkeley (bk) and r/c, was followed with genomic comparisons including recently described genomes of the tb phage from mexico (tbm) and pr phage to elucidate genomic diversity and candidate host range determinants. comparative whole genome analysis revealed high sequence homogeneity among these brucellaphage genomes and resolved three genetic groups consistent with defined host range pheno ... | 2014 | 24530704 |
| draft genome sequences of brucella suis biovar 4 strain nctc 10385, brucella ceti strain nctc 12891t, brucella inopinata strain camp 6436t, and brucella neotomae strain atcc 23459t. | with the aim of developing quantitative pcr methods for the detection and differentiation of brucella species, the genomes of brucella ceti, brucella inopinata, brucella netotomae, and brucella suis biovar 4 were sequenced and analyzed. | 2014 | 25278518 |
| immunotherapeutics to prevent the replication of brucella in a treatment failure mouse model. | outer membrane vesicles (omvs) from brucella melitensis and irradiated brucella neotomae have been shown to be effective vaccines against a b. melitensis challenge in a mouse model. the present study evaluates the efficacy of these two vaccines as immuno-therapeutics in combination with conventional antibiotics against a b. melitensis infection. balb/c mice chronically infected with b. melitensis were treated for 4 weeks with doxycycline and gentamicin and vaccinated twice during the course of t ... | 2014 | 24397905 |
| promotion and rescue of intracellular brucella neotomae replication during co-infection with legionella pneumophila. | we established a new brucella neotomaein vitro model system for study of type iv secretion system-dependent (t4ss) pathogenesis in the brucella genus. importantly, b. neotomae is a rodent pathogen and, unlike b. abortus, melitensis, and suis, has not been observed to infect humans. it therefore can be handled more facilely using biosafety level 2 practices. more particularly, using a series of novel fluorescent protein and lux operon reporter systems to differentially label pathogens and track i ... | 2017 | 28264909 |
| brucella neotomae infection in humans, costa rica. | several species of brucella are known to be zoonotic, but b. neotomae infection has been thought to be limited to wood rats. in 2008 and 2011, however, b. neotomae was isolated from cerebrospinal fluid of 2 men with neurobrucellosis. the nonzoonotic status of b. neotomae should be reassessed. | 2017 | 28518028 |