| cell-mediated immune response after brucella abortus s19 vaccination. | cell-mediated immunity to brucella abortus s19 vaccine was measured in young heifers by the microassay for stimulation of protein synthesis (sps) with [3h]leucine and the skin test for delayed hypersensitivity. brucella melitensis protein allergen and a crude b abortus s19-soluble antigen were compared in the sps test. the sps test was negative in 5 unvaccinated heifers and strongly positive in 3 twice-vaccinated steers. however, the sps test was positive only in 13 of 30 s19-vaccinated heifers ... | 1978 | 103470 |
| cell-mediated immune responses in cattle vaccinated with killed brucella melitensis strain h-38 vaccine or infected with viable brucella abortus strain 2308 organisms, or both. | | 1979 | 110181 |
| studies on the genetic control of murine humoral response to immunization with a peptidoglycan-containing fraction extracted from brucella melitensis. | a peptidoglycan containing fraction (fraction "5") extracted from brucella melitensis has been injected in low infra-vaccinating doses into inbred mice. the genetic control of the resulting anti-brucella humoral response has been studied in the c57bl/6 "good responder" x dba2 "low responder" model. the results observed in f1, f2 and reciprocal backcrosses show that the "good responder" character, although transmitted as a dominant trait, is under polygenic control and independent of h2 haplotype ... | 1979 | 118703 |
| [preservation of lyophilized brucella melitensis rev. 1 vaccine]. | | 1977 | 279055 |
| preliminary report on brucella melitensis, strain h-38 vaccine in cattle. | | 1977 | 286366 |
| on the growth of brucella species in presence of erythritol in defined and undefined media and amniotic fluid of human, cow and sheep. | different concentrations of erythritol (25-1000 microng/ml) in defined medium were shown to enhance the growth of 75.00 to 91.66 per cent of brucella abortus and 69.23 to 100.00 per cent of brucella melitensis strains studied. compared to these figures only 13.04 to 60.86% (in tryptose broth), and 41.40 to 62.50% (in glycerol dextrose broth), of brucella abortus strains, 25.00 to 83.30 per cent (in tryptose broth) and 83.30 to 100.00 per cent (in glycerol dextrose broth) of brucella melitensis s ... | 1977 | 403714 |
| rifampin in the treatment of experimental brucellosis in mice and guinea pigs. | rifampin, a broad-spectrum antibiotic able to penetrate intracellularly, was used for treatment of infections with brucella melitensis in mice and brucella abortus in guinea pigs. treatments were administered for seven, 14, or 21 days; mice were given 25 mg of rifampin/kg per day, and guinea pigs 100 mg/kg per day. efficacy of the drug was determined by comparison of rifampin-treated animals with saline-treated controls and with tetracycline-treated mice (200 mg/kg per day) according to the foll ... | 1977 | 409786 |
| [induction of auxotrophy in the brucellosis microbe under the effect of n-methyl-n'-nitro-n-nitrosoguannidine]. | mutagenic activity of n-methyl-n'-nitro-n-nitrosoguanidine with respect to brucella melitensis was studied. in the course of this study the method of obtaining auxotrophic brucella mutants was elaborated. numerous assortiment of auxotrophic mutants of different types was obtained. in a number of cases the acquisition of auxotrophy was accompanied by profound changes of the strain, such as the changes in morphology of colonies, in the biochemical activity, in the capacity of agglutination and in ... | 1978 | 414962 |
| successful treatment of brucella melitensis end-carditis. | brucella endocarditis is a rare, but often fatal, complication of brucellosis. a 32 year old man acquired brucellosis while on a visit to his former home in greece and presented six months later with malaise, fever and aortic regurgitation. blood cultures grew brucella melitensis biotype 1. combined chemotherapy with streptomycin, tetracycline and rifampin sterilized his blood; however, his aortic valve was replaced owing to recurrent emboli and cardiac failure. over the next 18 months the patie ... | 1978 | 645754 |
| [nitrofurantoin-test for the differentiation of bordetella bronchiseptica (author's transl)]. | bordetella bronchiseptica is primarily resistant against nitrofurantoin (mic greater than 200 mug/ml), and this feature can be used for the differentiation of the organism from other gram-negative coccobacteria. nitrofurantoin paper disks (300 mug) failed to affect the growth of 150 strains of b. bronchiseptica isolated from different animal hosts, but they produced marked inhibition zones in the cultures of the followingspecies: pasteurella multocida, pasteurella pneumotropica, pasteurella haem ... | 1975 | 809944 |
| evolution and taxonomy in the genus brucella: steroid hormone induction of filterable forms with altered characteristics after reversion. | several strains of brucella abortus, brucella suis, and brucella melitensis were exposed to physiologic concentrations of testosterone, progesterone, and diethylstilbestrol by incorporating them into the growth medium. the hormones induced brucella to form cell wall-defective organisms, including filterable forms. the filterable forms from 1 strain of b melitensis had altered characteristics when it reverted to growth as an intact cell. this is the 1st reported instance of induction of filterabl ... | 1976 | 816232 |
| killed vaccine in adjuvant and protection of mice against an intraperitoneal challenge of brucella: kinetic studies. | cd-i female mice were immunized with a subcutaneous injection of heat-inactivated brucella melitensis (strain 53 h 38) incorporated into a water-in-oil emulsion. one month later, the effectiveness of this immunization was investigated by studying quantitatively in the spleen the fate of an intraperitoneal challenge inoculum of approximately i x 10(6) viable b. abortus strain 544. in unvaccinated mice, the number of viable challenge bacteria increased until about the 10th day, decreased and then ... | 1976 | 829301 |
| vaccination of adult sheep with reduced doses of brucella melitensis strain rev.1: safety and serological responses. | a trial was made to test the safety of a brucella melitensis strain rev. 1 vaccine when used in various doses on pregnant sheep and a control group of pregnant goats. it was found that local sheep were more susceptible than goats to the effects of this vaccine, and that the accepted dosage rate of 10+5 viable organisms, as recommended for adult goats, should not be used on pregnant sheep. | 1977 | 906093 |
| isolation of brucella melitensis phage of broad biotype and species specificity. | a brucella melitensis phage was isolated. stocks of phage were established which produced early large plaques. the phage host range included all smooth brucella species. | 1976 | 947844 |
| titration of a brucella protein allergen in sheep sensitized with brucella melitensis. | a protein allergen extracted from a rough strain of b. melitensis was titrated in sheep sensitized by vaccination with the attenuated b. melitensis strain rev. i. single doses of allergen were given per animal by the intradermal or palpebral routes. doses over a 100-fold range produced delayed hypersensitivity reactions of similar magnitude by both routes. the most suitable method for application in the field was a dose of 50 mug given by the palpebral route (0.5 ml subcutaneously in the upper e ... | 1975 | 1163961 |
| brucellosis outbreak attributed to ingestion of umpasteurized goat cheese. | in six cases of brucella melitensis infections the common etiologic factor was the ingestion of raw goat cheese. presenting complaints were variable, but a constant feature was persistent fevers that frequently were present for months. evidence of liver cell dysfunction was present in each case, and in two, biopsy disclosed diffuse hepatitis with focal necrosis. the diagnoses were initially based on high titers of brucella agglutinins and later confirmed by positive blood cultures. a good clini ... | 1975 | 1170820 |
| [the intracellular multiplication of "brucella" the theory for an experimental model (author's transl)]. | brucella melitensis can penetrate and multiply in cells of the bhk21 line. the intracellular bacteria can be counted either by culture after destruction of the cells or by direct examination after staining; in the latter case, the cells are distributed into various classes according to the number of bacteria which they contain. the number of intracellular bacteria increased exponentially and the division time is 12 hours. the intracellular division of the bacteria is independent of the cellular ... | 1975 | 1200552 |
| [preparation and standarization of a brucellosis allergen of sheep and goats]. | the authors underline the consequences upon national economy and public health of sheep and goat brucellosis and expose the difficulties existing with the serological methods of diagnosis presently in use. in order to eliminate these disadvantages they propose the use of the intradermal allergic test reporting, at the same time, the reasons for which this test has not been until now universally adopted : lack of a standard allergen, always prepared by the same method and possessing excellent qua ... | 1975 | 1149929 |
| an unusual outbreak of brucellosis. | a brucella melitensis infection involved three persons. the infections were acquired by contact with a culture suspension that had been spilled during a laboratory exercise. one of the patients had only indirect contact with the spilled culture, probably contact with contaminated fomites. another of the patients developed a clinical remission before treatment with antibiotics and relapsed after six months. this patient also had a positive bone marrow culture when blood cultures were negative. th ... | 1975 | 1052665 |
| [a carboxylated latex agglutination test for the serological diagnosis of human and animal brucellosis]. | a carboxylated latex agglutination test was developed for detecting antibodies in sera of human and animals with brucellosis. the latex was chemico-linked with b. melitensis 16m extracted with 5% nacl. comparing with physico-sorbent latex antigens in detecting antibodies in sera of human and animals with brucellosis, the chemico-linked latex is more stable, more specific and more sensitive. in addition, preparation of chemico-linked latex and some factors affecting the result of the test were al ... | 1992 | 1300250 |
| evidence of reservior hosts of brucella mellitensis. | serological reactors to brucella melitensis were found among goats and eland in chilanga. the only clinical evidence of disease were abortion among the goats, and circumstances did not allow for isolation of the organism in the laboratory. no gross pathological lesions were observed on post mortem examination of the goats. the public health significance relates to the ease of br. melitensis transmission between reservoir hosts and human beings. | 1976 | 1052104 |
| stimulating activity of brucella fractions in a human lymphocyte transformation test. correlation with humoral and cellular immunity. | 'pi' a phenol-extracted fraction from brucella melitensis has been used for years in our laboratory for the vaccination of laboratory and agricultural workers considered 'at risk'. in the present work it has been used in vitro, together with another phenol-extracted and peptidoglycan containing fraction (4a) in a lymphocyte transformation test. correlation between stimulation index, humoral antibodies to brucella and the cutaneous response to pi have been studied in subjects vaccinated with the ... | 1976 | 992708 |
| [immunization of ewes against experimental brucella melitensis infection: comparison of eleven vaccines]. | a comparative study of 11 antibrucellic vaccines has been carried out in the limousin region of 360 ewe-lambs divided into groups of 30, a 12th group serving as control. after being vaccinated at 8 months, the ewe-lambs were covered between 9 1/2 and 10 1/2 months, then infected at 11 months with br. melitensis strain 53 h 38 by instillation of 4 x 10(6) germs on the conjonctiva. six of these vaccines reduced the number of abortions and the excretion of brucella at parturition: three consisted o ... | 1976 | 944151 |
| human brucellosis in kenya. | the serology, epidemiologic features and clinical presentation of 39 patients suffering from brucellosis in kenya are described. the prevalent species was brucella melitensis. there was a striking preference in the localisation of the infection for the spine and the hip joint. the disease runs a protracted course. phenomena due to hypersensitivity and neuropsychiatric disorders are discussed. advice regarding specific treatment is given. | 1976 | 941242 |
| biochemical mis-identification of brucella melitensis and subsequent laboratory-acquired infections. | brucella species are mis-identified in the api 20ne system as moraxella phenylpyruvica (profile number 1200004). since some brucella spp. grow readily in routine blood culture medium and may be isolated from patients without clinically obvious brucellosis, the risk of laboratory-acquired brucellosis exists. we describe two such cases. | 1992 | 1358958 |
| [letter: meningo-encephalitis and polyradiculoneuritis of brucellar origin. isolation of brucella melitensis in the c.s.f]. | | 1976 | 934882 |
| relation between macrophage migration inhibition and immunity to brucella abortus in guinea-pigs. | relation between macrophage migration inhibition and immunity to brucella abortus in guinea-pigs. onderstepoort journal of veterinary research 43 (4), 175-184 (1976). a soluble protein antigen was prepared from brucella melitensis rev i with which macrophage migration inhibition (mmi) assays were successfully done using guinea-pig peritoneal exudate cells. by comparing the mmi, agglutinin response and immunity of groups and of individual guinea-pigs which had been immunized with either b. melite ... | 1976 | 828715 |
| purification, characterization, and seroactivity of a 20-kilodalton brucella protein antigen. | an internal protein was purified from cell extracts of brucella melitensis b115 by a combination of preparative isoelectric focusing and high-performance size exclusion chromatography. the protein has an apparent molecular mass of 230 kda as determined by size exclusion chromatography. the protein was resolved to a single band of 20 kda after sodium dodecyl sulfate-polyacrylamide gel electrophoresis. the native protein had an isoelectric point of 4.9. the n-terminal sequence of the 20-kda protei ... | 1992 | 1400966 |
| priming of macaca cynomolgus philippinensis with purified antigen of brucella melitensis before injection of rev. i vaccine. | in experiments designed for study of bacteremia and serologic response of macaca cynomolgus philippinensis to vaccination with a soluble antigen (fraction i) of brucella melitensis and the live rev. i vaccine strain, fraction i and rev. i were administered both four weeks apart and simultaneously. one of three monkeys given the vaccines four weeks apart and two of three monkeys vaccinated with fraction i and rev. i in a single injection developed transient bacteremia, as shown by blood cultures. ... | 1976 | 824373 |
| [development of spleenic infection in the mouse following intravenous inoculation of various species of brucella in smooth (s) or rough (r) phases]. | the bacterial population in the spleen of mice inoculated intravenously by brucella abortus 544 (s), brucella suis 1330 (s), brucella melitensis 115 (r) or brucella canis rm 666(r) is studied kinetically. like the s strains of brucella, the r strains are able to multiply in the mouse spleen. the consequences for the tests of vaccines against natural infections produced by s or r strains of brucella are discussed. | 1975 | 810263 |
| a rapid and sensitive method for the identification of brucella species with a monoclonal antibody. | a coagglutination test using monoclonal antibody bme10-5 with specificity for the m antigen of brucella melitensis 16m has been developed for the rapid identification of the smooth brucella species. all reference strains of several biovars of b. melitensis, b. abortus, b. suis and b. neotomae tested were positive in this assay. no significant differences in reaction intensity were observed in relation to the different distribution of the a and m antigens among the brucella serovars analysed. con ... | 1992 | 1448627 |
| antibody response to brucella outer membrane proteins in bovine brucellosis: immunoblot analysis and competitive enzyme-linked immunosorbent assay using monoclonal antibodies. | sera from brucella-infected bovines were analyzed by immunoblotting by using sonicated cell extracts of b. melitensis or b. abortus and a competitive enzyme-linked immunosorbent assay (elisa) with monoclonal antibodies against outer membrane proteins (omps) with molecular masses of 10, 16.5, 19, 25 to 27, 36 to 38, and 89 kda. antibody responses against omps were compared with antibody responses against smooth lipopolysaccharide. immunoblot analysis indicated that the antibody response in infect ... | 1992 | 1452700 |
| immunization by an insoluble fraction extracted from brucella melitensis: immunological and chemical characterization of the active substances. | a peptidoglycan-containing fraction called fraction p.i. (phenol insoluble), extracted from brucella melitensis and previously described by some of us, had immunogenic and protective properties and did not produce any allergic reactions. since it is well known that bacterial peptidoglycans studied so far have immunoadjuvant properties, the isolation of the active factor(s) of brucella was undertaken. by successive enzymatic and chemical treatments, a new, much more purified fraction, called "4a" ... | 1976 | 770324 |
| studies on the immune protection to murine experimental brucellosis conferred by brucella fractions. i. positive role of immune serum. | mouse inoculation with three different phenol-insoluble fractions extracted from brucella melitensis (fractions 'pi', '4a' and '5') induces an acceleration of the blood clearance of i.v. inoculated live brucella and a diminution of the rate of multiplication of the injected bacteria in the spleen. preincubation of the challenge inoculum in immune serum or i.p. injections of immune serum confer a good specific protection to non-immunized hosts. the results observed with fractionated sera suggest ... | 1978 | 750371 |
| comparative in vitro activity of clarithromycin. spanish collaborative group. | the activity in vitro of clarithromycin, a new macrolide, was compared to that of various antibiotics in tests using 3,880 clinical isolates. clarithromycin was two times more active than erythromycin against staphylococcus aureus, enterococcus faecalis, enterococcus faecium, streptococci of groups c, g and f, brucella melitensis, legionella pneumophila and mycoplasma spp., 16 times more active against ureaplasma urealyticum and 2 to 4 times less active against campylobacter spp. in general, cla ... | 1992 | 1468430 |
| the ability of brucella melitensis to grow on loewenstein-jensen egg medium: presentation of a case with brucella meningitis. | | 1992 | 1468897 |
| serological response of adult goats infected with live brucella melitensis. | | 1978 | 719519 |
| inflammatory response in the placental attachments of the sheep following experimental infection with brucella melitensis. | | 1979 | 544456 |
| [identification and characterisation of 200 strains of brucella canis under test from china]. | 200 cultures of brucella canis under test were received from some provinces in our laboratory. 167 (85.59%) of them were identified as brucella according to morphology, growth properties and serologies. and then, they were proved as brucella canis with r-serum agglutination and lysed test of phage r/c. some strains also were performed dna homologous determination and observation of electron microscopic morphology. the results found out that the genetic constitution and picture of them identified ... | 1992 | 1481533 |
| [the spectrum of pathogens in positive blood cultures--tyrol 1991]. | in a recent study by the centers for disease control (cdc) it was noted that there had been a resurgence of gram-positive bacteremia together with an increase in fungemia. this reported trend is confirmed by data from the austrian tirol. in 1991 1,750 out of 13,679 specimens (12.8%) yielded bacterial or fungal growth, accounting for 1,248 cases of "bacteremia"; no decision was made about the clinical significance of the culture isolates. we consider laboratory reports of blood isolates to be fai ... | 1992 | 1481545 |
| brucella melitensis hepatitis: the absence of granulomas. | | 1979 | 475172 |
| [endocarditis caused by brucella melitensis on a mitral valve prosthesis presenting as an ischemic cerebrovascular accident]. | a new case of endocarditis by brucella melitensis on a mitral valve prosthesis in a 15 year old patient, whose first manifestation was an ischemic cerebrovascular accident is reported. the patient presented with daily fever only two months later. medical treatment alone was not sufficient to avoid valvular failure and substitution of the prosthesis was required. the clinical manifestations and complications of this infrequent condition is discussed. treatment, which often requires the combinatio ... | 1992 | 1489778 |
| [allergic diagnosis of bovine brucellosis. 1. conditions for the use of a purified protein allergen: brucellin (author's transl)]. | a protein allergen extracted from the rough strain brucella melitensis b 115, prepared for the diagnosis of brucellosis, has been evaluated in cows. injected in non infected and non vaccinated cows, this allergen does not give any reaction, does not sensitize for subsequent injections, does not give rise to antibodies detected by routine serologic tests, and thus does not interfere with usual screening. in cows, sensitized by injections of h 38 or 45/20 vaccines, the intradermal injection produc ... | 1977 | 413466 |
| [evolution of antibiotic sensitivity in brucella melitensis]. | | 1992 | 1498179 |
| enumeration and identification of human leukemic lymphocytes by their natural binding of bacteria. | the recently described property of bacteria to bind to human lymphocytes was used to distinguish between normal and chronic leukemic lymphocyte (cll) populations. strains of the following bacteria were used in this study: arizona hinshawii, escherichia coli strains 1 and 2, bacillus globigii, brucella melitensis, corynebacterium diphtheriae strains 1 and 2, corynebacterium xerosis, sarcina lutea, staphylococcus aureus, and staphylococcus epidermidis. for identification of immunoglobulin-bearing ... | 1977 | 404035 |
| [cachexia in mice induced by a fraction of brucella melitensis. modification by levamisole or by diethyldithiocarbamate of soda]. | the pmf fraction of b. melitensis creates a wasting disease in newborn mice (j. infect. dis., 1973, 127, p. 139). administered to adult mice, pmf induces a 4 to 8 g loss of weight in 20 g mice and more markedly in males than in females. treatment with levamisole (lms) suppresses this difference in response to pmf and restores normal weight in 24 days. diethyldithiocarbamate (ddc), another immunostimulant sulfur derivate, abolishes the emaciating effect of pmf. furthermore, ddc is an anabolizing ... | 1976 | 177323 |
| preliminary studies on the use of carbon substrate utilization patterns for identification of brucella species. | a commercial system (biolog, hayward, ca) that uses reduction of an indicator dye to determine the oxidation of 95 different carbon substrates contained in a defined minimal medium was tested with 35 isolates of brucella spp. to determine if the system could be used in place of respirometric methods to identify species. of 95 substrates contained in this system, three were oxidized by all the brucella strains tested, 48 were oxidized by none of the strains tested, and 44 were oxidized differenti ... | 1992 | 1572134 |
| comparison of brucella abortus and brucella melitensis infections of mice and their effect on acquired cellular resistance. | by using mice infected with strains of brucella abortus and brucella melitensis we examined the histological responses to infection, the relationship of histology to persistence of organisms, and the relation of persistence of organisms to the acquisition of acquired cellular resistance (acr). infection with b. abortus resulted in well-formed granulomas in the livers, which persisted for more than 30 days. in contrast, infection with b. melitensis produced microabscesses in the livers which reso ... | 1979 | 121113 |
| properties of the berkeley phage lytic for "brucella melitensis" and other species. | | 1978 | 37809 |
| evaluation of polysaccharide, lipopolysaccharide, and beta-glucan antigens in gel immunodiffusion tests for brucellosis in cattle. | in venezuela, 1,012 cattle sera were screened for their ability to precipitate brucella melitensis 16m smooth-lipopolysaccharide (s-lps), b melitensis b115 polysaccharide b (poly b), b abortus 1119-3 o-polysaccharide (ps), or b abortus 1119-3 cyclic 1,2 linked beta-d-glucan (beta-glucan) in an agar-gel immunodiffusion assay. these sera were previously classified as being brucella abortus-infected, s-19-vaccinated, or negative after an assessment of historical records and results of 5 standard se ... | 1992 | 1595966 |
| [the genus brucella, its nomenclature and taxonomic specificity (author's transl)]. | the author suggest to change the nomenclature of genus-brucella as follows: brucella melitensis, b. bovis, b. suis, b. canis, b. neotomae, b. rangiferi, b. murium. in the new edition of "bergey's manual" it is suggested to quote some highly taxonomic specific substances, berucellaphages, brucelline, protective substance, precipitinogen, and toxins (endotoxin, lipoprotein). | 1976 | 1274499 |
| [chemical fractionation of brucella melitensis and the immunizing properties of a new fraction]. | a complex phenol-insoluble fraction (fraction p.i.) extracted from brucella melitensis presents immunizing properties already described by the authors. this fraction contains peptidoglycane whose immuno-adjuvant properties are known in numerous bacterial species. more precise fractionation was carried out to determine if the properties observed after injection of p.i. are in fact specific or on the contrary due to peptidoglycane action. by chemical and enzymatic treatments, 90% of the p.i. are e ... | 1976 | 1261754 |
| brucellosis as a world problem. | brucellosis is one of the most widespread and economically the most ravaging of zoonoses. the occurrence of the acute, often incapacitating infection in man caused by brucella melitensis usually coincides with occurrence of the infection in sheep and goats. well-known foci of this infection have been identified in the mediterranean basin, central asia and latin america. recent investigations in parts of africa and india have shown that the infection is much more widespread than was previously su ... | 1976 | 1261753 |
| microbiological test strip (api20ne) identifies brucella melitensis as moraxella phenylpyruvica. | | 1991 | 1669878 |
| brucellosis surveillance. | | 1991 | 1669892 |
| [preparation and testing of different antibrucellic vaccines]. | from a highly virulent brucella melitensis strain of human origin, different vaccines of good antigenic quality are obtained, ranging from the very agglutinogenic oily type vaccine to the totally non-agglutinogenic oily type vaccine. the non-agglutinogenic vaccine is obtained by an original method consisting of submission of the original brucella to several subcultures in the presence of a small quantity of antiserum and clonal selection of a population. the strain finally selected is totally no ... | 1976 | 1261748 |
| transmission of brucella melitensis. | | 1991 | 1672931 |
| brucellosis linked to mexican cheese. | three cases of brucellosis occurred, two acute cases and one chronic, all of which likely resulted from the ingestion of fresh cheese containing brucella melitensis. a history of patients with illness suggestive of brucellosis should include details about food ingested recently in addition to the usual questions about travel and occupation. as the us domestic-animal disease comes under better control, and travel to mexico, spain, and italy increases, it seems likely that human disease caused by ... | 1975 | 1173155 |
| [the clinico-epidemiological characteristics of brucellosis in young people]. | the article describes an outbreak of brucellosis among the servicemen (age 19-32) which was caused by br. melitensis. 13% of servicemen in the military unit went through this disease. this outbreak was caused by unsatisfactorily treated mutton of sheep which were affected with brucellosis. the illness had acute septic course. the appearance of the focuses of disease in locomotor system took place only when the infectious process had a rather long period (septicometastatic course). the acute sept ... | 1992 | 1292191 |
| control of brucellosis in kuwait by vaccination of cattle, sheep and goats with brucella abortus strain 19 or brucella melitensis strain rev. 1. | in kuwait, approximately 12,000 dairy cows were vaccinated with a reduced dose of 3 x 10(9) brucella abortus strain 19 and approximately 350,000 sexually mature sheep and goats with a reduced dose of 10(7) b. melitensis strain rev. 1. using the criteria of prevaccinal and postvaccinal incidences of antibodies, abortions, and human cases of brucellosis, the programme was very successful. widespread vaccination of adult animals is the most effective method of controlling brucellosis among cattle, ... | 1992 | 1306918 |
| brucella melitensis splenic abscess: sonographic detection and follow-up. | | 1992 | 1316379 |
| rapid diagnosis of brucella melitensis in blood: some operational characteristics of the bact/alert. | a clinical isolate of brucella melitensis was detected in a blood culture with the bact/alert after an incubation period of 2.8 days. dilution studies revealed an inverse linear relationship between the log of the initial concentration of the organism and the time to detection of a positive result. reproducibility studies demonstrated a mean detection time of 48 +/- 1 h in 8 of 10 replicates seeded with a stock containing 10(2) cfu/ml. | 1992 | 1734057 |
| in vitro activity of azithromycin and tetracycline against 358 clinical isolates of brucella melitensis. | the in vitro activity of azithromycin against human pathogenic strains of brucella melitensis was tested at three centres and compared to that of tetracycline, the standard antibiotic currently used for the treatment of human brucellosis. mic determination was carried out. tested concentration ranges for both azithromycin and tetracycline were between 0.03 and 16 micrograms/ml. brucella melitensis biotype 1, strain m16 was employed as a control microorganism. a total of 358 brucella melitensis s ... | 1992 | 1317801 |
| correspondence analysis in medical research. | various applications of correspondence analysis to biomedical data are presented. the basic concepts of profile, mass and chi-squared distance are introduced in an initial simple example using data on the relationship between headache types and age. the main result of the correspondence analysis is a geometric map of this relationship showing how the relative frequencies of headache types change with age. a second example maps the association between personality types and various medical diagnos ... | 1992 | 1341654 |
| protection against brucella melitensis or brucella abortus in mice with immunoglobulin g (igg), iga, and igm monoclonal antibodies specific for a common epitope shared by the brucella a and m smooth lipopolysaccharides. | mice passively immunized prior to a challenge infection with immunoglobulin g (igg) and igm monoclonal antibodies (mabs) specific for a common epitope of both a- and m-dominant strains had viable brucella abortus 544 or brucella melitensis h38 counts in the spleen reduced to the same extent as did mice passively immunized with mabs specific for either the a or the m epitope. the iga mab was not effective. | 1992 | 1370276 |
| characterization of smooth brucella lipopolysaccharides and polysaccharides by monoclonal antibodies. | two mouse monoclonal antibodies (mab) generated to the m antigen of brucella melitensis 16m were analysed. binding profiles of both monoclonals were established by a competitive enzyme-linked immunosorbent assay using chemically defined lipopolysaccharides, o polysaccharides and native hapten polysaccharides from b. melitensis 16m, b. abortus 544 and yersinia enterocolitica o:9. using this assay, significant differences in the reactivity of both antibodies were found with a and m antigens from b ... | 1991 | 1805311 |
| o-chain expression in the rough brucella melitensis strain b115: induction of o-polysaccharide-specific monoclonal antibodies and intracellular localization demonstrated by immunoelectron microscopy. | spleen cells from mice infected with the rough brucella melitensis strain b115 were fused with nso myeloma cells. hybridoma supernatants were screened in elisa with cell walls (cw), sonicated cell extracts (ce) and rough lipopolysaccharide (r-lps) of b. melitensis strain b115 and whole b. melitensis b115 cells. surprisingly, 22 monoclonal antibodies (mabs) reacting in elisa with both cw and ce but not with r-lps and bacterial cells were shown by immunoblot analysis and elisa to react with smooth ... | 1992 | 1382111 |
| induction of antibody and protective responses in mice by brucella o-polysaccharide-bsa conjugate. | a brucella conjugate vaccine was synthesized by covalently coupling the o-polysaccharide (ps) obtained from brucella melitensis 16m, to bovine serum albumin (bsa). the conjugate (ps-bsa) was non-toxic for mice. when injected at two doses (10 or 1 micrograms) in mice, it induced the production of antibodies and the boost was shown to be efficient if administered after 10 weeks. immunization of mice with 10 micrograms of conjugate conferred significant protection against virulent challenge with b. ... | 1991 | 1811374 |
| [osteoarticular manifestations of brucellosis. apropos of 2 cases of brucellosis-induced sacroiliitis]. | | 1992 | 1411027 |
| [elaboration of a rapid method of determining the sensitivity of brucella to antibiotics]. | optimal conditions were developed for determination of antibiotic sensitivity in brucella by using enzyme immunoassay directly in the primary cultures of the material tested. the brucella concentration in the material tested should be not lower than 1.10(6) microbial cells/ml and the time of culture incubation be 24 hours at 37 degrees c. the obligatory condition is to use a liquid medium, i.e. the albimi broth with 1% glucose. to inhibit the foreign microflora it is recommended to use polymyxin ... | 1992 | 1417320 |
| [serological survey of small ruminants in 4 districts of niger]. | in 1990, a serosurvey on 1,474 small ruminants was conducted in four districts of niger (maradi, zinder, diffa and dosso). the epidemiology and seroprevalence of eight diseases were studied: brucella melitensis and b. ovis brucellosis, chlamydiosis, coxiellosis, contagious caprine pleuropneumonia, type a pasteurellosis, peste des petits ruminants, rift valley fever, crimée-congo haemorragic fever. the main health problem for the development of small ruminants farming, seems to be both pasteurell ... | 1991 | 1843821 |
| isolation of brucella melitensis from aborting ruminants in jordan. | thirty-four isolates (16.5 per cent) of brucella melitensis were cultured from 206 samples from aborted, still-born or weak full-term animals and vaginal swabs from aborted animals. twenty-six of 31 isolates from sheep were b. melitensis biovar 3. two isolates were biovar 1 and one of these was the vaccine strain rev 1. the three remaining isolates were from vaginal swabs and were not biotyped since b. melitensis biovar 3 had been isolated from the aborted fetuses. one of two isolates from cattl ... | 1992 | 1452817 |
| brucellosis with nephrotic syndrome, nephritis and iga nephropathy. | a patient with systemic brucellosis due to brucella melitensis had severe renal involvement. clinical features included hypertension, macroscopic haematuria, massive proteinuria of 10 g per 24 hours and azotaemia. following treatment with antibiotics, the azotaemia resolved and proteinuria decreased to less than 0.5 g per 24 hours, but microscopic haematuria and hypertension persisted. renal biopsy during recovery revealed iga nephropathy with minimal mesangial changes, suggesting a causal relat ... | 1992 | 1461859 |
| infections in british clinical laboratories, 1988-1989. | during 1988-89 this continuing survey showed 18 infections in the staff of laboratories reporting from 166 centres, representing 21,756 person-years of exposure. shigella and other bowel infections (one caused by s typhi) predominated, affecting 11 microbiology medical laboratory scientific officers. three shigella infections originated from quality control samples. pulmonary tuberculosis affected four workers, including two mortuary technicians, but without detected occupational exposure to myc ... | 1991 | 1890201 |
| [sensitivity of brucella melitensis to various antibiotics, drug preparations and their combinations]. | in vitro activity of 13 antibiotics and drug preparations as well as some of their combinations was studied with respect to brucella melitensis. it was shown that the strains of b. melitensis were sensitive to sisomicin, amikacin, gentamicin, cefotaxime, rifaprim +, nitroxoline and trimethoprim. variations in the doxycycline sensitivity depending on the strain were observed. the strains of b. melitensis were resistant to cephalexin, phosphomycin, chinoxydine, dioxydine and oxolinic acid. combina ... | 1991 | 1892428 |
| serological and bacteriological study of brucellosis in camels in central saudi arabia. | sera from 2,630 apparently normal adult camels (camelus dromedarius) raised in central saudi arabia (riyadh and al-kharj cities) were examined serologically by the rose bengal and standard united states of america brucella plate agglutination tests. the overall seroprevalence of brucellosis in the restricted populations of tested camels was 8%. the seroprevalence of brucellosis among camels raised in small numbers in the backyards of 24 houses in riyadh and those intensively raised on one large ... | 1992 | 1472730 |
| in vitro susceptibility of brucella melitensis to antibiotics. | the in vitro susceptibilities of 86 recent clinical isolates of brucella melitensis to minocycline, streptomycin, co-trimoxazole, rifampin, and six fluoroquinolones were determined. minocycline exhibited the lowest mic and was followed by rifampin and streptomycin. among the quinolones, win 57273 and ciprofloxacin were the most active agents. no antibiotic combination of these agents exhibited synergy against 15 selected isolates. in killing rate experiments, streptomycin exhibited the most rapi ... | 1991 | 1952869 |
| treatment of brucella melitensis infection in sheep and goats with oxytetracycline combined with streptomycin. | six treatment regimens using oxytetracycline (otc) combined with streptomycin (st) were evaluated for eliminating brucella melitensis from 480 naturally-infected sheep and goats. cessation of shedding brucella from udder secretions and absence of brucella in selected tissues at autopsy were considered criteria for successful treatment. four regimens were equally effective in eliminating brucella in the treated groups of sheep and goats regardless of the source of antibiotics used. these were reg ... | 1992 | 1472731 |
| unrecognised neurobrucellosis giving rise to brucella melitensis peritonitis via a ventriculoperitoneal shunt. | | 1992 | 1486897 |
| detection of brucella melitensis and brucella abortus by dna amplification. | suitable reaction conditions and oligonucleotide primers were sought for the detection of brucella melitensis and brucella abortus by the polymerase chain reaction. primers were chosen from within the coding sequence of a gene encoding a 31 kda b. abortus antigen. the test was shown to be sensitive, and specificity was demonstrated using dna derived from a panel of gram-negative pathogens. there was no detectable difference between b. melitensis and b. abortus in the sensitivity of the reaction ... | 1992 | 1495123 |
| antibacterial activity of fce 22101 against relatively resistant blood culture isolates. | in vitro antibacterial activity of the new penem fce 22101 was tested against blood culture isolates from 1,374 consecutive patients in a major tertiary care referral center in saudi arabia. many of the isolates were significantly more resistant to commonly used beta lactams. of the 809 members of enterobacteriaceae tested all but 14 isolates of enterobacter were susceptible to fce 22101 with an mic range of 0.12-8.0 micrograms/ml. it inhibited all the 183 isolates of staphylococcus aureus, both ... | 1991 | 2013238 |
| bilateral mammary abscess and uveitis caused by brucella melitensis--report of a case. | soft tissue locations of brucella are a rare finding. we report the isolation of brucella melitensis from a suspected breast tumour of a woman who also showed signs of uveitis. three weeks after surgical drainage and despite antimicrobial therapy a new abscess developed on the other breast whose culture also yielded brucella. as observed, unusual localization of brucellosis without previous penetrating injury may be the only manifestation of chronic infection. | 1991 | 2013508 |
| therapy of experimental murine brucellosis with streptomycin, co-trimoxazole, ciprofloxacin, ofloxacin, pefloxacin, doxycycline, and rifampin. | mice infected with brucella melitensis were treated with streptomycin, co-trimoxazole, ciprofloxacin, doxycycline, and rifampin intraperitoneally and with ciprofloxacin, ofloxacin, pefloxacin, doxycycline, and rifampin orally for 14 to 21 days. doxycycline- and rifampin-treated animals (either route) demonstrated a cure rate significantly better than that of controls. longer therapy periods were associated with a significantly better outcome. therapy failure was observed in all mice treated with ... | 1992 | 1510422 |
| an elisa with brucella lipopolysaccharide antigen for the diagnosis of b. melitensis infection in sheep and for the evaluation of serological responses following subcutaneous or conjunctival b. melitensis strain rev 1 vaccination. | an indirect enzyme-linked immunosorbent assay (elisa) with unpurified brucella melitensis smooth lipopolysaccharide (s-lps) as antigen was evaluated for the serological diagnosis of b. melitensis infection in sheep in comparison with the rose bengal (rb), complement fixation (cf), radial immunodiffusion (rid), microplate agglutination (ma) and rivanol agglutination (riv) tests. tests rb and cf detected as positive each of the 77 sera from b. melitensis-infected animals tested, the rid (74), ma ( ... | 1992 | 1557898 |
| identification of dye-sensitive strains of brucella melitensis. | strains of brucella melitensis biovars 1, 2, and 3 isolated in india, italy, kuwait, saudi arabia, the federal republic of germany, and zimbabwe were inhibited by thionin (20 micrograms/ml) but not by basic fuchsin (20 micrograms/ml) or safranin o (200 micrograms/ml). in other respects the strains were typical of their species and biovars. the existence of dye-sensitive strains of b. melitensis suggests that the present system of classification requires modification. | 1991 | 2056043 |
| brucellosis induced avascular necrosis of the femoral head in a 7 year old child. | the case is reported of a 7 year old bedouin girl who presented with a three week history of fever and limp. initial tests for rheumatoid factor, antinuclear antibodies, and brucella were negative. two weeks after admission brucella melitensis was cultured from bone marrow and synovial fluid samples. the patient improved after treatment with doxycillin and streptomycin, but radiographs of the right hip showed avascular necrosis of the right femoral head. a mild limp and limitation of motion in t ... | 1992 | 1575594 |
| conjunctival vaccination of pregnant ewes and goats with brucella melitensis rev 1 vaccine: safety and serological responses. | when brucella melitensis strain rev 1 vaccine (rev 1) is administered by the standard method (1-2 x 10(9) viable bacteria injected subcutaneously), it may induce long-lasting serological responses and/or cause abortion in pregnant animals. the conjunctival route considerably reduces these drawbacks. in the present experiment a 1 x 10(8) cfu dose for both ewes and goats conjunctivally vaccinated at mid-pregnancy was tested for innocuousness (outcome of pregnancy, contamination of unvaccinated con ... | 1992 | 1610080 |
| inhibition of the degranulation and myeloperoxidase activity of human polymorphonuclear neutrophils by brucella melitensis. | the degranulation and myeloperoxidase-h2o2-halide activities of human polymorphonuclear leukocytes from healthy donors were tested after co-incubation with either brucella melitensis 16m, staphylococcus aureus or staphylococcus aureus in presence of lipopolysaccharide, protein fraction, native hapten and soluble fractions released at 65 degrees c from smooth strain of brucella melitensis 16m. the degranulation and myeloperoxidase activities of polymorphonuclear leukocytes were significantly high ... | 1991 | 1662050 |
| [brucellosis in a 3 month-old infant]. | during the course of a bronchiolitis in a 3 month-old child, one blood culture yielded brucella melitensis. the diagnosis of brucellosis is difficult in infancy because the symptoms are nonspecific and the disease may be mild and self-limited. the child received only breast milk and dietetic preparations. so, the detection in the maternal serum of a significant titer of brucella agglutinin enabled us to consider the very rare human-to-human transmission from mother to child. because the efficacy ... | 1990 | 2078109 |
| person-to-person transmission of brucella melitensis. | human brucellosis is primarily an occupational hazard in the usa; in the middle east and africa ingestion of contaminated dairy products is an important route of infection. whether human beings can become infected via person-to-person spread is uncertain. during an investigation of a commonsource, laboratory-associated outbreak due to brucella melitensis, biotype 3, the wife of a microbiologist with serologically proven brucellosis became infected. her blood isolate was indistinguishable from th ... | 1991 | 1670649 |
| failure of prolonged treatment with ciprofloxacin in acute infections due to brucella melitensis. | a randomized prospective, pilot study was performed to compare the efficacy of oral ciprofloxacin (750 mg or 1000 mg bd) with standard oral antimicrobial therapy (rifampicin plus doxycycline) in the treatment of acute infection with brucella melitensis. all antimicrobial drugs were administered for 42 days. although all patients responded rapidly, five of the six patients receiving ciprofloxacin relapsed following cessation of therapy. there were no relapses among the patients who received doxyc ... | 1990 | 2081722 |
| failure of ciprofloxacin to eradicate brucellosis in experimental animals. | ciprofloxacin is a quinolone that penetrates well intracellularly. it was shown to be active against brucella melitensis in vitro. in this study, ciprofloxacin and tetracycline were used to treat b. melitensis and b. abortus infections in mice. the drugs were given for 7, 14, 21 and 28 days. tetracycline reduced spleen and liver weight and splenic bacterial counts and eliminated the infection in 70% of mice treated for 3 or 4 weeks. ciprofloxacin, on the other hand, did not affect liver or splee ... | 1990 | 2093110 |
| antigenic relationships of the lipopolysaccharides of escherichia hermannii strains with those of escherichia coli o157:h7, brucella melitensis, and brucella abortus. | clinical isolates of escherichia hermannii which showed serological cross-reaction with polyclonal antisera to the o-polysaccharide portion of the lipopolysaccharide of e. coli o157 strains and with antisera to the o antigens of brucella abortus and b. melitensis were found by chemical and nuclear magnetic resonance analyses to have lipopolysaccharide o chains composed of linear polymers containing 1,2- and 1,3-linked 4-acetamido-4,6-dideoxy-alpha-d-mannopyranosyl (alpha-d-rhap4nac) residues. tw ... | 1990 | 1691146 |
| experimental ovine salmonellosis (salmonella abortusovis): pathogenesis and vaccination. | salmonella enterica subsp. enterica ser. abortusovis, a sheep-adapted serotype, causes a contagious disease. abortion is the major symptom and the main source of contamination. research on this ovine disease may aid farmers, but may also contribute to comparative biological knowledge. innate resistance partly controlled by the ity locus, increased resistance to reinfection and humoral and t-cell-mediated immunity were observations gained with a murine model. in ewes, abortion regularly occurs fo ... | 1990 | 2101486 |
| thyroiditis due to brucella melitensis--report of two cases. | two cases of thyroiditis due to brucella melitensis are reported. both occurred in foreigners working in switzerland and presented with characteristic symptoms of thyroiditis which masked those of brucellosis. cultures were diagnostic. one patient had concomitant thyroid malignancy. both showed normal thyroid function and were cured following antibiotic treatment. | 1990 | 2114370 |
| brucella suis s2, brucella melitensis rev. 1 and brucella abortus s19 living vaccines: residual virulence and immunity induced against three brucella species challenge strains in mice. | live attenuated brucella suis s2 vaccine was compared to living vaccines b. abortus s19 and b. melitensis rev. 1 in mice. residual virulence was estimated by ability to multiply and persist in spleen and lymph nodes. immunogenicity was estimated by spleen counts of control and vaccinated mice challenged either with the reference b. abortus 544 strain or with virulent b. melitensis h38 and b. suis 1330 strains. s2 vaccine had lower residual virulence; expressed as 50% recovery time, persistence w ... | 1990 | 2123586 |
| [simultaneous isolation of brucella melitensis and mycobacterium tuberculosis in pleural empyema]. | | 1990 | 2129196 |
| [cloning of brucella genes in escherichia coli k12 cells and analysis of products of the cloned genes]. | the libraries of brucella melitensis 565 and brucella abortus 99 in escherichia coli cells have been constructed. some clones of escherichia coli producing the specific brucella antigens have been found in immunological tests with brucella antiserum. two strains producing antigens have been characterized, one being from brucella melitensis 565 and another from brucella abortus 99 clone libraries . both strains synthesize two antigens that were studied by immunoelectrophoresis, immunoblotting aft ... | 1990 | 1701518 |
| probable breast-milk borne brucellosis in a young infant. | a young infant with acute brucellosis is reported. he presented with a septicaemia-like picture. diagnosis was based on a fourfold rise of brucella agglutination titres and a positive blood culture. he had been exclusively breast-fed when his mother developed brucellosis 4 weeks after delivery. it is strongly suspected that the transmission of brucella melitensis to this infant was through the maternal breast-milk. | 1990 | 1703749 |
| [the antigenic relationship between brucella abortus, brucella melitensis and yersinia enterocolitica serotype 0:3 and 0:9]. | antigenic interrelationship between b. abortus, b. melitensis, y. enterocolitica serotype 0:3 and serotype 0:9 were investigated in hyperimmün sera obtained from rabbits, by using agglutination and agglutinin absorbtion tests. agglutination was determined at 1/2560 titer of b. abortus and b. melitensis hyperimmün sera when their own antigens were used. on the other hand, these sera agglutinated y. enterocolitica serotype 0:9 antigen at 1/1280 and 1/320 titer respectively. as it was seen that hig ... | 1990 | 1704479 |