| phenotypic variation of pseudomonas putida and p. tolaasii affects attachment to agaricus bisporus mycelium. | the effect of phenotypic variation on attachment of pseudomonas tolaasii and p. putida to agaricus bisporus mycelium was investigated. quantitative studies demonstrated the ability of each isolate to attach rapidly and firmly to a. bisporus mycelium and significant differences in attachment of wild-type and phenotypic variant strains were observed. this was most pronounced in p. tolaasii, where the percentage attachment of the wild-type form was always greater than that of the phenotypic variant ... | 1991 | 1791432 |
| left handed alpha-helix formation by a bacterial peptide. | the alpha-helix is a common element of secondary structure in proteins and peptides. in eukaryotic organisms, which exclusively incorporate l-amino acids into such molecules, stereochemical interactions make such alpha-helices, invariably right-handed. pseudomonas tolaasii paine is the causal organism of the economically significant brown blotch disease of the cultivated mushroom agaricus bisporus (lange) imbach. p. tolaasii proceduces an extracellular lipodepsipeptide toxin, tolaasin, which cau ... | 1991 | 1991518 |
| bacterial siderophores: structures of pyoverdins pt, siderophores of pseudomonas tolaasii ncppb 2192, and pyoverdins pf, siderophores of pseudomonas fluorescens ccm 2798. identification of an unusual natural amino acid. | pyoverdins were isolated and characterized respectively from the cultures of pseudomonas tolaasii ncppb 2192 (pyoverdins pt, pt a, and pt b) and pseudomonas fluorescens ccm 2798 (pyoverdins pf/1, pf/2, pf, pf/3/1, and pf/3/2) each grown in iron-deficient conditions. their structures were established by using fab-ms, nmr, and cd techniques. these siderophores are chromopeptides, and all but one (pyoverdin pf/3/3) possess at the n-terminal end of their peptide chain the same chromophore that has b ... | 1990 | 2125501 |
| specificity of pyoverdine-mediated iron uptake among fluorescent pseudomonas strains. | pyoverdine-mediated iron transport was determined for seven fluorescent pseudomonas strains belonging to different species. for all strains, cell or cell outer membrane and iron(iii)-pyoverdine combinations were compared with their homologous counterparts in uptake, binding, and cross-feeding experiments. for four strains (pseudomonas putida atcc 12633, pseudomonas fluorescens w, p. fluorescens atcc 17400, and pseudomonas tolaasii ncppb 2192), the pyoverdine-mediated iron transport appeared to b ... | 1988 | 3170485 |
| bacteria antagonistic to pseudomonas tolaasii and their control of brown blotch of the cultivated mushroom agaricus bisporus. | | 1972 | 4628501 |
| bacteria-mediated uptake of choline sulfate by plants: bacterial effectiveness. | the ability of bacteria to cause rapid uptake of choline sulfate in plants, i.e., effectiveness, was studied using pseudomonas tolaasii and excised roots of barley (hordeum vulgare l.). once effective, bacteria remained so after being killed by treatments which cause little damage to their outer structure. however, effectiveness was destroyed by disruption of the cell wall, protein reagents, a mild heat treatment or removal of mg2+. effective bacteria adsorbed choline sulfate. this adsorption ha ... | 1980 | 7397169 |
| identification and characterization of a locus which regulates multiple functions in pseudomonas tolaasii, the cause of brown blotch disease of agaricus bisporus. | pseudomonas tolaasii, the causal agent of brown blotch disease of agaricus bisporus, spontaneously gives rise to morphologically distinct stable sectors, referred to as the phenotypic variant form, at the margins of the wild-type colonies. the phenotypic variant form is nonpathogenic and differs from the wild type in a range of biochemical and physiological characteristics. a genomic cosmid clone (psisg29) from a wild-type p. tolaasii library was shown to be capable of restoring a range of chara ... | 1995 | 7642492 |
| identification of a gene cluster encoding three high-molecular-weight proteins, which is required for synthesis of tolaasin by the mushroom pathogen pseudomonas tolaasii. | the extracellular lipodepsipeptide toxin tolaasin is the primary disease determinant of pathogenicity of pseudomonas tolaasii on the cultivated mushroom, agaricus bisporus. transposon mutagenesis of p. tolaasii ncppb 1116 with tn5-generated 5000 chromosomal insertions of which 35 (0.7%) were tolaasin negative and 12 (0.25%) produced a reduced amount of tolaasin. in addition, tnphoa mutagenesis yielded a single tolaasin-negative mutant which was phoa active. restriction enzyme mapping of mutant d ... | 1993 | 8332057 |
| pseudomonas tolaasii: extra-genomic factor mediates toxin production and efficiency. | pseudomonas tolaasii is responsible for brown blotch symptoms on agaricus bisporus. investigations among 15 p. tolaasii wild-type strains revealed the existence of an extra-genomic factor. pseudomonas tolaasii cnbp2152d, a spontaneous derivative of the wild-type strain cnbp2152 which has lost the factor, exhibited a significant decline in pathogenicity. comparison of blotch symptoms induced by toxins extracted from cnbp2152 and cnbp2152d showed that the extragenomic factor mediates toxin product ... | 1997 | 9252589 |
| spontaneous duplication of a 661 bp element within a two-component sensor regulator gene causes phenotypic switching in colonies of pseudomonas tolaasii, cause of brown blotch disease of mushrooms. | spontaneous sectoring of pseudomonas tolaasii colonies results in a phenotypic switch from the smooth, pathogenic form (designated 1116s) to the rough non-pathogenic form (designated 1116r). this phenotypic switch can also be induced by mutation of the phen master regulatory locus, which encodes a 99 kda protein with homology to the conserved family of sensor regulator proteins. southern blot analysis of genomic dna from 1116s and 1116r probed with a 3.4 kb xhol-bamhi fragment containing the phe ... | 1997 | 9282733 |
| preliminary description of biocidal (syringomycin) activity in fluorescent plant pathogenic pseudomonas species. | strains representing the fluorescent plant pathogenic pseudomonas spp., ps. agarici, ps. asplenii, ps. avellanae, ps. beteli, ps. caricapapayae, ps. cichorii, ps. corrugata, ps. ficuserectae, ps. flectens, ps. fuscovaginae, ps. marginalis, ps. meliae, ps. savastanoi, ps. syringae, ps. tolaasii and ps. viridiflava were tested for biocidal activity using aspergillus niger as assay organism. inhibitory behaviour was found in strains of ps. asplenii, ps. blatchfordae, ps. cichorii, ps. corrugata, ps ... | 1998 | 9750309 |
| a quantitative trait locus of agaricus bisporus resistance to pseudomonas tolaasii is closely linked to natural cap color. | a quantitative trait locus (qtl) of resistance to pseudomonas tolaasii was detected in agaricus bisporus using a cross between a wild strain from the sonoran desert and a cultivated strain. the resistance qtl was strongly linked with the brown color allele of ppc1. this qtl explained about 30% of the variation observed for living bacteria-induced symptoms. the use of bacterial toxin did not reproduce living bacteria symptoms but revealed the same qtl. the latter qtl was not affected by environme ... | 1999 | 10512670 |
| biochemical and physiological aspects of brown blotch disease of agaricus bisporus. | pseudomonas tolaasii is a bacterium endemic to the compost beds where common mushroom (agaricus bisporus) is cultivated. under some environmental conditions still not well-determined, but influenced by temperature and relative humidity, the bacterium can become pathogenic and provoke the brown blotch disease. this review describes the interaction between p. tolaasii and a. bisporus that results in the appearance of brown spots on the mushroom caps, typical symptoms of the disease. firstly, p. to ... | 1999 | 10525168 |
| analysis of the role of reca in phenotypic switching of pseudomonas tolaasii. | switching between the pathogenic smooth (1116s) and nonpathogenic rough (1116r) forms of pseudomonas tolaasii occurs due to the reversible duplication of a 661-bp element within the phen locus. disruption of the chromosomal reca locus of 1116s and 1116r produced strains 1116sreca and 1116rreca, respectively, which showed typical loss of uv resistance. switching from the smooth to the rough form was virtually eliminated in the 1116sreca strain, whereas the extent of switching from the rough to th ... | 2000 | 11053404 |
| application of siderotyping for characterization of pseudomonas tolaasii and "pseudomonas reactans" isolates associated with brown blotch disease of cultivated mushrooms. | pyoverdine isoelectric focusing analysis and pyoverdine-mediated iron uptake were used as siderotyping methods to analyze a collection of 57 northern and central european isolates of p. tolaasii and "p. reactans." the bacteria, isolated from cultivated agaricus bisporus or pleurotus ostreatus mushroom sporophores presenting brown blotch disease symptoms, were identified according to the white line test (w. c. wong and t. f. preece, j. appl. bacteriol. 47:401-407, 1979) and their pathogenicity to ... | 2000 | 11055932 |
| the structure of a pyoverdine produced by a pseudomonas tolaasii-like isolate. | cultures of agaricus bisporus, the most extensively cultivated mushroom, can be infected severely by pseudomonas tolaasii. this pathogen is characterized by the so-called white line reaction, a precipitate formed on agar plates between its colonies and those of p. reactans, both belonging to the collective species p. fluorescens. a recent study has shown that a group of p. tolaasii isolates can be subdivided into two groups or 'siderovars', based on the pyoverdines they produce (munsch et al. 20 ... | 2001 | 11368279 |
| characterization by 16s rrna sequence analysis of pseudomonads causing blotch disease of cultivated agaricus bisporus. | bacterial blotch of agaricus bisporus has typically been identified as being caused by either pseudomonas tolaasii (brown blotch) or pseudomonas gingeri (ginger blotch). to address the relatedness of pseudomonads able to induce blotch, a pilot study was initiated in which pseudomonads were selectively isolated from mushroom farms throughout new zealand. thirty-three pseudomonad isolates were identified as being capable of causing different degrees of discoloration (separable into nine categories ... | 2001 | 11526038 |
| genetic characterization of pseudomonas 'nzi7'--a novel pathogen that results in a brown blotch disease of agaricus bisporus. | to characterize a novel pseudomonad isolate capable of causing brown blotch disease of agaricus bisporus. | 2001 | 11556905 |
| evaluation of bacteria isolated from rice for plant growth promotion and biological control of seedling disease of rice. | of 102 rhizoplane and endophytic bacteria isolated from rice roots and stems in california, 37% significantly (p < or = 0.05) inhibited the growth in vitro of two pathogens, achlya klebsiana and pythium spinosum, causing seedling disease of rice. four endophytic strains were highly effective against seedling disease in growth pouch assays, and these were identified as pseudomonas fluorescens (s3), pseudomonas tolaasii (s20), pseudomonas veronii (s21), and sphingomonas trueperi (s12) by sequencin ... | 2001 | 11718545 |
| the white-line-in-agar test is not specific for the two cultivated mushroom associated pseudomonads, pseudomonas tolaasii and pseudomonas "reactans". | a sharply defined white line in vitro forms between the pathogenic form of pseudomonas tolaasii and another pseudomonas bacterium, referred to as "reactans". this interaction has been considered as highly specific. however, results presented in this study rise doubt about the strict specificity of this interaction, as some other pseudomonads, associated with the cultivated mushroom agaricus bisporus, also yielded a white line precipitate when they were streaked towards pseudomonas tolaasii lmg 2 ... | 2002 | 11911616 |
| evidence for genotypic differences between the two siderovars of pseudomonas tolaasii, cause of brown blotch disease of the cultivated mushroom agaricus bisporus. | sixteen representative isolates of pseudomonas tolaasii, the causal agent of brown blotch of the cultivated mushroom agaricus bisporus, were previously assigned to two siderovars (sv1 and sv2) on the basis of pyoverdines synthesized. each isolate was pathogenic and produced a typical white line precipitate when cultured adjacent to pseudomonas "reactans" strain lmg 5329. these 16 isolates of p. tolaasii, representing sv1 and sv2, were further characterized using genotypic methods to examine the ... | 2002 | 12002406 |
| siderophore typing, a powerful tool for the identification of fluorescent and nonfluorescent pseudomonads. | a total of 301 strains of fluorescent pseudomonads previously characterized by conventional phenotypic and/or genomic taxonomic methods were analyzed through siderotyping, i.e., by the isoelectrophoretic characterization of their main siderophores and pyoverdines and determination of the pyoverdine-mediated iron uptake specificity of the strains. as a general rule, strains within a well-circumscribed taxonomic group, namely the species pseudomonas brassicacearum, pseudomonas fuscovaginae, pseudo ... | 2002 | 12039729 |
| pcr assays for specific and sensitive detection of pseudomonas tolaasii, the cause of brown blotch disease of mushrooms. | the present study describes pcr assays to detect specifically pseudomonas tolaasii from various samples. | 2002 | 12358687 |
| identification of non-pseudomonad bacteria from fruit bodies of wild agaricales fungi that detoxify tolaasin produced by pseudomonas tolaasii. | bacterial isolates from wild agaricales fungi detoxified tolaasin, the inducer of brown blotch disease of cultivated mushrooms produced by pseudomonas tolaasii. mycetocola tolaasinivorans and mycetocola lacteus were associated with fruit bodies of wild pleurotus ostreatus and wild lepista nuda, respectively. tolaasin-detoxifying bacteria belonging to other genera were found in various wild mushrooms. an acinetobacter sp. was isolated from fruit bodies of tricholoma matsutake, bacillus pumilus wa ... | 2002 | 12450133 |
| several pseudomonads, associated with the cultivated mushrooms agaricus bisporus or pleurotus sp., are hemolytic. | pseudomonas tolaasii, causing brown blotch disease on cultivated mushrooms, and yielding a white line precipitate towards p. "reactans", has been shown to induce lysis of erythrocytes. some finnish strains isolated from diseased mushroom fruit bodies, although harboring the typical features of p. tolaasii, proved to be distinct, and have been allocated to a nov. sp. p. costantinii. we examined in these study whether all brown blotch causing agents were hemolytic. the induction of erythrocytes ly ... | 2002 | 12501995 |
| pseudomonas costantinii sp. nov., another causal agent of brown blotch disease, isolated from cultivated mushroom sporophores in finland. | pathogenic bacteria are frequently associated with mushroom sporophores exhibiting brown blotch disease symptoms. these bacteria belong mainly to pseudomonas tolaasii or occasionally to 'pseudomonas reactans'. although a group of isolates originating from some finnish mushroom farms satisfied the two characteristic criteria for diagnosis of infection with p. tolaasii (i.e. yielding a typical brown blotch symptom on agaricus bisporus sporophores and producing a typical white line in agar when str ... | 2002 | 12508856 |
| pseudomonas extremorientalis sp. nov., isolated from a drinking water reservoir. | on the basis of phenotypic and genotypic characteristics and 16s rdna sequence analysis, a novel species belonging to the genus pseudomonas sensu stricto was identified. the saprophytic, fluorescent bacterium, designated kmm 3447(t), was isolated from a drinking water reservoir near vladivostok city, russia. the novel organism was a gram-negative, aerobic, rod-shaped bacterium that produced a cyclic depsipeptide with surface-active properties. it degraded casein, but did not degrade gelatin, sta ... | 2002 | 12508877 |
| two types of ion channel formation of tolaasin, a pseudomonas peptide toxin. | tolaasin is a peptide toxin produced by pseudomonas tolaasii and causes brown blotch disease of the cultivated mushrooms. two types of ion channels were identified by the incorporation of tolaasin into lipid bilayer. the slope conductance of type 1 channel measured in the buffer containing 100 mm kcl was 150 ps with a linear current vs. voltage relationship. the type 2 tolaasin channel had two subconductance states of 300 and 500 ps. both channels were inhibited by zn(2+). ion channel formations ... | 2003 | 12725930 |
| structural determination of the o-specific chain of the lipopolysaccharide from the mushrooms pathogenic bacterium pseudomonas tolaasii. | the complete structure of the o-specific polysaccharide of the lipopolysaccharide isolated from the cultivated mushrooms pathogen pseudomonas tolaasii is described. the structural determination, achieved by chemical and spectroscopical analyses, indicates a novel tetrasaccharide repeating unit built up of two units of 2-acetamido-2,6-di-deoxy-glucopyranose (quinovosamine, quipnac) and two units of 2-acetamido-2-deoxy-gulopyranuronamide (gulpnacan), one of which is acetylated at c-3 position: | 2003 | 12747869 |
| a left-handed alpha-helix containing both l- and d-amino acids: the solution structure of the antimicrobial lipodepsipeptide tolaasin. | the 18-amino acid cytolytic lipodepsipeptide tolaasin, produced in culture by virulent strains of pseudomonas tolaasii, is the causal agent of the brown blotch disease of the cultivated mushroom. tolaasin has a sequence of d-amino acids in its n-terminal region, then alternates l- and d-amino acids, and bears a c-terminal lactone macrocycle composed of 5-residues. the solution structure of tolaasin in sodium dodecyl sulfate was studied by 2d-nmr spectroscopy and molecular dynamics simulated anne ... | 2003 | 12910453 |
| interaction between gfp-tagged pseudomonas tolaasii p12 and pleurotus eryngii. | pseudomonas sp., (formerly reported as strain p12) which produces brown blotch disease symptoms on pleurotus eryngii, has been identified as p. tolaasii based on its biochemical, physiological properties and 16s rdna sequence analysis. this pathogen is able to infect basidiocarps when surface-inoculated on mushroom casing soil. however, infected basidiocarps develop the brown blotch disease symptoms when the pathogen concentration in the fruiting body tissues is higher than 10(4) cfu/g d.w. usin ... | 2003 | 14521237 |
| tolaasins a--e, five new lipodepsipeptides produced by pseudomonas tolaasii. | pseudomonas tolaasii, the causal organism of brown blotch disease of agaricus bisporus and of the yellowing of pleurotus ostreatus, was shown to produce in culture tolaasin i (1), tolaasin ii (2), and five other minor metabolites, tolaasins a, b, c, d, and e (3-7). these compounds were demonstrated to be important in the development of the disease symptoms. this paper reports on the structural elucidation, based essentially on nmr studies and ms spectra, and biological activity of the above lipo ... | 2004 | 15165142 |
| the structure of the phosphorylated carbohydrate backbone of the lipopolysaccharide of the phytopathogen bacterium pseudomonas tolaasii. | a novel core-lipid a backbone oligosaccharide was isolated and identified from the lipopolysaccharide fraction of the mushrooms pathogen bacterium pseudomonas tolaasii. the oligosaccharide was obtained by alkaline treatment of the lipopolysaccharide fraction. since the repeating unit of the o-antigen contained one residue of -->4)-alpha-l-gulpnacan, the hydrolysis was accompanied by beta-elimination on this residue and following depolymerization, producing a mixture of oligosaccharides. the comp ... | 2004 | 15337452 |
| antimicrobial activity of streptomyces species against mushroom blotch disease pathogen. | bacterial blotch caused by pseudomonas tolaasii is still one of the main diseases in mushroom farms. effective treatment of the infections caused by p. tolaasii is yet to be established. the isolation and identification of three streptomyces species that showed antimicrobial activity against p. tolaasii ncppb 2192(t) were described. six strains were highly active with an inhibition zone more than 20 mm in diameter. they were assigned to the known streptomyces cluster groups as s. rochei, s. lydi ... | 2005 | 15678564 |
| novel lectin-like bacteriocins of biocontrol strain pseudomonas fluorescens pf-5. | bacteriocin llpa, produced by pseudomonas sp. strain bw11m1, is a peculiar antibacterial protein due to its homology to mannose-binding lectins mostly found in monocots (a. h. a. parret, g. schoofs, p. proost, and r. de mot, j. bacteriol. 185:897-908, 2003). biocontrol strain pseudomonas fluorescens pf-5 contains two llpa-like genes, named llpa1(pf-5) and llpa2(pf-5). recombinant escherichia coli cells expressing llpa1(pf-5) or llpa2(pf-5) acquired bacteriocin activity and secreted a 31-kda prot ... | 2005 | 16151105 |
| analysis of rhizobacterial communities in perennial graminaceae from polluted water meadow soil, and screening of metal-resistant, potentially plant growth-promoting bacteria. | this study investigates the impact of long-term heavy metal contamination on the culturable, heterotrophic, functional and genetic diversity of rhizobacterial communities of perennial grasses in water meadow soil. the culturable heterotrophic diversity was investigated by colony appearance on solid lb medium. genetic diversity was measured as bands in denaturing gradient gel electrophoresis (dgge) obtained directly from rhizosphere soil and rhizoplane dna extracts, and from the corresponding cul ... | 2004 | 16329902 |
| wlip and tolaasin i, lipodepsipeptides from pseudomonas reactans and pseudomonas tolaasii, permeabilise model membranes. | the activity of the white line inducing principle (wlip) and tolaasin i, produced by virulent strains of pseudomonas reactans and pseudomonas tolaasii, respectively, was comparatively evaluated on lipid membranes. both lipodepsipeptides were able to induce the release of calcein from large unilamellar vesicles. their activity was dependent on the toxin concentration and liposome composition and in particular it increased with the sphingomyelin content of the membrane. studies of dynamic light sc ... | 2006 | 16925979 |
| yield, size and bacterial blotch resistance of pleurotus eryngii grown on cottonseed hulls/oak sawdust supplemented with manganese, copper and whole ground soybean. | experiments were performed to determine effects of supplementation of cottonseed hull/sawdust substrate with mn, cu, and ground soybean on yield, mushroom size, and bacterial blotch resistance of two commercial strains of pleurotus eryngii. a basal formulation (d.w.) of cottonseed hulls (62%), aged red oak sawdust (27%), whole ground soybean (6%), corn distiller's waste (4%) and calcium sulfate (1%) was supplemented to 50, 150 or 250 microg/g mn or cu and to 4%, 8% and 12% whole ground soybean. ... | 2007 | 16973354 |
| purification of a pore-forming peptide toxin, tolaasin, produced by pseudomonas tolaasii 6264. | tolaasin, a pore-forming peptide toxin, is produced by pseudomonas tolaasii and causes brown blotch disease of the cultivated mushrooms. p. tolaasii 6264 was isolated from the oyster mushroom damaged by the disease in korean. in order to isolate tolaasin molecules, the supernatant of bacterial culture was harvested at the stationary phase of growth. tolaasin was prepared by ammonium sulfate precipitation and three steps of chromatograpies, including a gel permeation and two ion exchange chromato ... | 2007 | 17244491 |
| identification of compounds exhibiting inhibitory activity toward the pseudomonas tolaasii toxin tolaasin i using in silico docking calculations, nmr binding assays, and in vitro hemolytic activity assays. | using in silico docking calculations, nmr analysis of target-ligand binding, and hemolytic activity assays, we searched a 30,000-compound library for an effective inhibitor of tolaasin i, a pseudomonas tolaasii toxin that causes virulent infection in mushrooms. of more than 30,000 compounds screened in silico, two compounds were selected. one of these compounds, sorbitololeic acid, bound to tolaasin i and inhibited its hemolytic activity in vitro. therefore, sorbitololeic acid can be a potential ... | 2009 | 19500984 |
| bioactive and structural metabolites of pseudomonas and burkholderia species causal agents of cultivated mushrooms diseases. | pseudomonas tolaasii, p. reactans and burkholderia gladioli pv. agaricicola, are responsible of diseases on some species of cultivated mushrooms. the main bioactive metabolites produced by both pseudomonas strains are the lipodepsipeptides (ldps) tolaasin i and ii and the so called white line inducing principle (wlip), respectively, ldps which have been extensively studied for their role in the disease process and for their biological properties. in particular, their antimicrobial activity and t ... | 2008 | 19787100 |
| temperature-dependent hemolytic activity of membrane pore-forming peptide toxin, tolaasin. | tolaasin, a pore-forming peptide toxin produced by pseudomonas tolaasii, causes brown blotch disease on cultivated mushrooms. hemolysis using red blood cells was measured to evaluate the cytotoxicity of tolaasin. to investigate the mechanism of tolaasin-induced cell disruption, we studied the effect of temperature on the hemolytic process. at 4 degrees celsius, poor binding of the tolaasin molecules to the erythrocyte membrane was observed and most of the tolaasin molecules stayed in the solutio ... | 2010 | 19960443 |
| microbially induced diseases of agaricus bisporus: biochemical mechanisms and impact on commercial mushroom production. | the button mushroom, agaricus bisporus (lange) imbach, the most common cultivated mushroom, is susceptible to a wide range of virus, bacterial, and fungal diseases. however, only some diseases were studied for the mechanisms involved in the host-microorganism interaction. this review deals with biochemical mechanisms related to cavity disease (burkholderia gladioli) and to the interaction between a. bisporus and the causal agents responsible for the most severe diseases, namely the bacteria pseu ... | 2010 | 20127233 |
| characterization of pseudomonads isolated from decaying sporocarps of oyster mushroom. | pleurotus ostreatus is one of the most extensively cultivated mushrooms in the world; however, the success of cultivation often depends on the proliferation of different bacterial pathogens. pseudomonas tolaasii is thought as the major cause of brown blotch disease of agaricus bisporus and yellowing of pleurotus ostreatus. in this study we examined the pathogenicity and assessed the industrial damage causing effect of 41 pseudomonas strains isolated from deformed, yellowing oyster mushroom (p. o ... | 2011 | 20627228 |
| correlation of maple sap composition with bacterial and fungal communities determined by multiplex automated ribosomal intergenic spacer analysis (marisa). | during collection, maple sap is contaminated by bacteria and fungi that subsequently colonize the tubing system. the bacterial microbiota has been more characterized than the fungal microbiota, but the impact of both components on maple sap quality remains unclear. this study focused on identifying bacterial and fungal members of maple sap and correlating microbiota composition with maple sap properties. a multiplex automated ribosomal intergenic spacer analysis (marisa) method was developed to ... | 2011 | 21569942 |
| Acylhomoserine lactone production by bacteria associated with cultivated mushrooms. | The main bacterial pathogens of cultivated mushroom as well as mushroom-associated bacteria, which were isolated from Agaricus bisporus, Pleurotus ostreatus and Pleurotus eryngii mushroom niches, were evaluated for the production of N-acyl-L-homoserine lactones (AHLs) by using four bioreporters. Furthermore, identification of AHLs by LC-ESI-FTICR MS was performed on culture filtrates of selected pathogens and mushroom-associated bacteria strains, which resulted in inducing at least one of the fo ... | 2011 | 21942309 |
| In silico study of the ion channel formed by tolaasin I produced by Pseudomonas tolaasii. | A toxin produced by Pseudomonas tolaasii, tolaasin, causes brown blotch disease in mushrooms. Tolaasin forms pores on the cellular membrane and destroys cell structure. Inhibiting the ability of tolaasin to form ion channels may be an effective method to protect against attack by tolaasin. However, it is first necessary to elucidate the three-dimensional structure of the ion channels formed by tolaasin. In this study, the structure of the tolaasin ion channel was determined in silico based on da ... | 2011 | 22031037 |
| distinct lipopeptide production systems for wlip (white line-inducing principle) in pseudomonas fluorescens and pseudomonas putida. | the interaction of wlip (white line-inducing principle), a member of the viscosin group of pseudomonas lipopeptides, with tolaasin, a lipopeptide mycotoxin secreted by pseudomonas tolaasii, enables identification of the mushroom pathogen relying on formation of a lipopeptide coprecipitate between confronted colonies of an indicator strain (designated pseudomonas 'reactans') and p. tolaasii. the wlip non-ribosomal lipopeptide synthesis system of the mushroom isolate p. 'reactans' lmg 5329 (wip) w ... | 2012 | 23757145 |
| draft genome sequence of pseudomonas fluorescens lmg 5329, a white line-inducing principle-producing bioindicator for the mushroom pathogen pseudomonas tolaasii. | pseudomonas tolaasii, the causative agent of agaricus bisporus brown blotch disease, can be identified by the white line reaction, occurring upon confrontation of the tolaasin-producing mushroom pathogen with "pseudomonas reactans," producing the lipopeptide white line-inducing principle (wlip). the draft genome sequence of the wlip-producing indicator pseudomonas fluorescens strain lmg 5329 is reported here. | 2013 | 23887909 |
| cyclic lipodepsipeptides produced by pseudomonas spp. naturally present in raw milk induce inhibitory effects on microbiological inhibitor assays for antibiotic residue screening. | two pseudomonas strains, identified as closely related to pseudomonas tolaasii, were isolated from milk of a farm with frequent false-positive delvotest results for screening putative antibiotic residues in raw milk executed as part of the regulatory quality programme. growth at 5 to 7°c of these isolates in milk resulted in high lipolysis and the production of bacterial inhibitors. the two main bacterial inhibitors have a molecular weight of 1168.7 and 1140.7 da respectively, are heat-tolerant ... | 2014 | 24853676 |
| genetic and functional characterization of cyclic lipopeptide white-line-inducing principle (wlip) production by rice rhizosphere isolate pseudomonas putida rw10s2. | the secondary metabolite mediating the gacs-dependent growth-inhibitory effect exerted by the rice rhizosphere isolate pseudomonas putida rw10s2 on phytopathogenic xanthomonas species was identified as white-line-inducing principle (wlip), a member of the viscosin group of cyclic lipononadepsipeptides. wlip producers are commonly referred to by the taxonomically invalid name "pseudomonas reactans," based on their capacity to reveal the presence of a nearby colony of pseudomonas tolaasii by induc ... | 2012 | 22544260 |
| bdellovibrio bacteriovorus hd100 guards against pseudomonas tolaasii brown-blotch lesions on the surface of post-harvest agaricus bisporus supermarket mushrooms. | pseudomonas tolaasii is a problematic pathogen of cultured mushrooms, forming dark brown 'blotches' on mushroom surfaces and causing spoilage during crop growth and post-harvest . treating p. tolaasii infection is difficult, as other, commensal bacterial species such as pseudomonas putida are necessary for mushroom growth, so treatments must be relatively specific. | 2014 | 24946855 |
| inactivation of gram-positive bacteria by novel phenolic branched-chain fatty acids. | novel phenolic branched-chain fatty acids (pbc-fas) were evaluated for their antimicrobial properties against both gram-positive ( listeria innocua , bacillus subtilis , enterococcus faecium ) and gram-negative ( escherichia coli , salmonella typhimurium, and pseudomonas tolaasii ) bacteria. in addition, pbc-fa derivatives, such as pbc-fa methyl ester mixture, methyl-branched fatty acid mixtures, and trimethylsilyl-pbc-fa methyl esters, were synthesized to study the structure activity relationsh ... | 2017 | 28221889 |
| toxicity of twenty-two plant essential oils against pathogenic bacteria of vegetables and mushrooms. | asbtract toxicity of twenty-two essential oils to three bacterial pathogens in different horticultural systems: xanthomonas campestris pv. phaseoli (causing blight of bean), clavibacter michiganensis subsp. michiganensis (bacterial wilt and canker of tomato), and pseudomonas tolaasii (causal agent of bacterial brown blotch on cultivated mushrooms) was tested. control of bacterial diseases is very difficult due to antibiotic resistance and ineffectiveness of chemical products, to that essential o ... | 2016 | 27494458 |
| pseudomonas protegens pf-5 causes discoloration and pitting of mushroom caps due to the production of antifungal metabolites. | bacteria in the diverse pseudomonas fluorescens group include rhizosphere inhabitants known for their antifungal metabolite production and biological control of plant disease, such as pseudomonas protegens pf-5, and mushroom pathogens, such as pseudomonas tolaasii. here, we report that strain pf-5 causes brown, sunken lesions on peeled caps of the button mushroom (agaricus bisporus) that resemble brown blotch symptoms caused by p. tolaasii. strain pf-5 produces six known antifungal metabolites u ... | 2014 | 24742073 |
| to settle or to move? the interplay between two classes of cyclic lipopeptides in the biocontrol strain pseudomonas cmr12a. | pseudomonas cmr12a is a biocontrol strain that produces phenazine antibiotics and as yet uncharacterized cyclic lipopeptides (clps). the clps of cmr12a were studied by chemical structure analysis and in silico analysis of the gene clusters encoding the non-ribosomal peptide synthetases responsible for clp biosynthesis. cmr12a produces two different classes of clps: orfamides b, d and e, whereby the latter two represent new derivatives of the orfamide family, and sessilins a-c. the orfamides are ... | 2014 | 24673852 |
| pathogenic variation in isolates of pseudomonas causing the brown blotch of cultivated mushroom, agaricus bisporus. | twenty seven bacterial isolates were isolated from superficial brown discolorations on the caps of cultivated agaricus bisporus. after white line assay (wla) and the assist of biolog computer-identification system, isolates were divided into groups: (i) comprised ninteen bacterial isolates that positively responded to a pseudomonas "reactans" reference strain (ncppb1311) in wla and were identified as pseudomonas tolaasii, (ii) comprised two isolates which were wla+ towards the reference strain ( ... | 2012 | 24031938 |
| [identification and characterization of a hypothermia nitrite bacterium pseudomonas tolaasii y-11]. | denitrifying bacteria play an important role in the biological nitrogen removal process. however, there are few studies about hypothermia nitrite denitrifying bacteria. we isolated a hypothermia and aerobic nitrite-denitrifying bacterium (named as y-11) from the long-term flooded paddy soil. aims of this paper were to clarify the phylogeny and denitrifying characteristics of strain y-11. | 2015 | 26665596 |
| heterotrophic nitrification and aerobic denitrification by pseudomonas tolaasii y-11 without nitrite accumulation during nitrogen conversion. | a hypothermia aerobic nitrite-denitrifying bacterium, pseudomonas tolaasii strain y-11, was found to display high removal capabilities for heterotrophic nitrification with ammonium and for aerobic denitrification with nitrate or nitrite nitrogen. when strain y-11 was cultivated for 4days at 15°c with the initial ammonium, nitrate and nitrite nitrogen concentrations of 209.62, 204.61 and 204.33mg/l (ph 7.2), the ammonium, nitrate and nitrite removal efficiencies were 93.6%, 93.5% and 81.9% withou ... | 2016 | 26519702 |
| bioactivity of volatile organic compounds produced by pseudomonas tolaasii. | pseudomonas tolaasii is the main bacterial pathogen of several mushroom species. in this paper we report that strains of p. tolaasii produce volatile substances inducing in vitro mycelia growth inhibition of pleurotus ostreatus and p. eryngii, and agaricus bisporus and p. ostreatus basidiome tissue blocks brown discoloration. p. tolaasii strains produced the volatile ammonia but not hydrogen cyanide. among the volatiles detected by gc-ms, methanethiol, dimethyl disulfide (dmds), and 1-undecene w ... | 2015 | 26500627 |
| common virulence factors for pseudomonas tolaasii pathogenesis in agaricus and arabidopsis. | brown blotch of cultivatable mushrooms is a disease caused by the small peptide toxin (tolaasin) secreted by pseudomonas tolaasii. here we found that the wild type tolassin-producing p. tolaasii stain 6264 was capable of infection in arabidopsis thaliana cotyledons, causing chlorotic symptoms and growth arrest as a result of bacterial proliferation. seven virulence-attenuated mutants of p. tolaasii were isolated from the agaricus bisporus screen using 2512 mariner-based transposon insertion muta ... | 2014 | 24370573 |
| characterization of bacteriophage φpto-bp6g, a novel phage that lyses pseudomonas tolaasii causing brown blotch disease in mushrooms. | the bacteriophage, φpto-bp6g, exhibited strong bactericidal activity against pseudomonas tolaasii, the bacterium that causes brown blotch disease in cultivated mushrooms. analysis of phage morphology with an electron microscope revealed that φpto-bp6g contains an icosahedral head and a long tail, which is classified as the family of siphoviridae. the phage was observed to lyse p. tolaasii in the broth about 4h after inoculation, indicating a putative lytic pathway exists during bacterial growth. ... | 2012 | 23041492 |
| isolation of new pseudomonas tolaasii bacteriophages and genomic investigation of the lytic phage bf7. | sixteen lytic bacteriophages that infect pseudomonas tolaasii lmg 2342(t) were isolated from smashed sporocarps of oyster mushroom (pleurotus ostreatus) showing necrotic symptoms. on the basis of the host range investigation of the phages, they have wide infection abilities against the genus pseudomonas, mainly in the case of phages bf3, bf7, bf10, and bf15. molecular investigations have revealed that they all have dsdna genomes about 40 kbp in size. identical restriction patterns resulting from ... | 2012 | 22568623 |
| in vitro activity of antimicrobial agents against pseudomonas tolaasii, pathogen of cultivated button mushroom. | in vitro antibacterial activity tests of seven biofungicides (ekstrasol, bisolbisan, bisolbifit, serenade, sonata, timorex, f-stop) and two disinfectants (colloidal silver alone and in combination with hydrogen peroxide) against the pseudomonas tolaasii strain (ns3b6) were carried out by the disc-diffusion, broth microdilution and broth macrodilution method. biofungicides tested in this study did not exhibit any antimicrobial activity in neither one of the methods used. disc diffusion method rev ... | 2012 | 22375589 |
| coregulation of the cyclic lipopeptides orfamide and sessilin in the biocontrol strain pseudomonas sp. cmr12a. | cyclic lipopeptides (clps) are synthesized by nonribosomal peptide synthetases (nrps), which are often flanked by luxr-type transcriptional regulators. pseudomonas sp. cmr12a, an effective biocontrol strain, produces two different classes of clps namely sessilins and orfamides. the orfamide biosynthesis gene cluster is flanked up- and downstream by luxr-type regulatory genes designated ofar1 and ofar2, respectively, whereas the sessilin biosynthesis gene cluster has one luxr-type regulatory gene ... | 2017 | 28621084 |