| characterization of maize polyamine oxidase. | some structural and biochemical characteristics of polyamine oxidase (pao) purified from maize shoots have been examined. the enzyme has only alanine as n-terminal amino acid and its n-terminal sequence shows a significant degree of homology with tryptophan 2-monooxygenase from pseudomonas syringae pv. savastanoi. the ph optimum for the stability of the native enzyme is 5, similar to that of the barley leaf enzyme. calorimetric analysis shows a single two-state transition at ph 6 with tm 49.8 de ... | 1990 | 1366693 |
| evidence for a correlation between auxin production and host plant species among strains of pseudomonas syringae subsp. savastanoi. | auxin production by 131 strains of pseudomonas syringae subsp. savastanoi was investigated with the aim of looking for correlations among this characteristic and the origin of the strains, the types of symptoms, and the host plant. most of the p.syringae subsp. savastanoi strains, except those isolated from ash, produced auxin and harbored iaa genes. among ash strains, which were pathogenic only on ash, only 2 out of 33 were found to produce auxin and to harbor iaa genes. | 1992 | 1622252 |
| limited host range ti plasmids: recent origin from wide host range ti plasmids and involvement of a novel is element, is868. | agrobacterium tumefaciens biotype iii octopine strains have been isolated from grapevine tumors worldwide. they comprise limited and wide host range (lhr and whr) strains that carry related tumor-inducing (ti) plasmids with two t-regions, ta and tb. the whr ta-region resembles the biotype i octopine region, whereas the lhr ta-region is a recent deletion derivative of the whr ta-region, which lacks the iaa genes and part of the ipt gene. sequencing of the ta-region of the ubiquitous lhr strain ab ... | 1991 | 1657255 |
| indole-3-acetic acid (iaa) synthesis in the biocontrol strain cha0 of pseudomonas fluorescens: role of tryptophan side chain oxidase. | pseudomonas fluorescens strain cha0 is an effective biocontrol agent against soil-borne fungal plant pathogens. in this study, indole-3-acetic acid (iaa) biosynthesis in strain cha0 was investigated. two key enzyme activities were found to be involved: tryptophan side chain oxidase (tso) and tryptophan transaminase. tso was induced in the stationary growth phase. by fractionation of a cell extract of strain cha0 on deae-sepharose, two distinct peaks of constitutive tryptophan transaminase activi ... | 1991 | 1663150 |
| genetic and functional analysis of the basic replicon of pps10, a plasmid specific for pseudomonas isolated from pseudomonas syringae patovar savastanoi. | the sequence of a 1823 base-pair region containing the replication functions of pps10, a narrow host-range plasmid isolated from a strain of pseudomonas savastanoi, is reported. the origin of replication, oriv, or pps10 is contained in a 535 base-pair fragment of this sequence that can replicate in the presence of trans-acting function(s) of the plasmid. oriv contains four iterons of 22 base-pairs that are preceded by g+c-rich and a+t-rich regions. a dnaa box located adjacent to the repeats of t ... | 1992 | 1738155 |
| the indoleacetic acid-lysine synthetase gene of pseudomonas syringae subsp. savastanoi induces developmental alterations in transgenic tobacco and potato plants. | the iaal gene of pseudomonas syringae subsp. savastanoi encodes an indoleacetic acid-lysine synthetase that conjugates lysine to indoleacetic acid. a chimaeric gene consisting of the iaal coding region under the control of the 35s rna promoter from cauliflower mosaic virus (35siaal) has been used to test if iaal gene expression leads to morphological alterations in tobacco and potato. transgenic tobacco plantlets bearing this construct have been shown to synthesize iaa-[14c]lysine when fed with ... | 1991 | 1905782 |
| the tr-dna region carrying the auxin synthesis genes of the agrobacterium rhizogenes agropine-type plasmid pria4: nucleotide sequence analysis and introduction into tobacco plants. | we have determined the nucleotide sequence of a 6-kilobase fragment of the agrobacterium rhizogenes plasmid pria4 tr-region that carries genes (aux1 and aux2) responsible for auxin biosynthesis in transformed plant cells. sequence analysis revealed two open reading frames corresponding to proteins of 749 amino acids for the aux1 gene and 466 amino acids for the aux2 gene. we observed significant similarity between the amino acid sequences deduced from the pria4 aux genes and those of the auxin b ... | 1991 | 1932811 |
| molecular characterization and expression analysis of the anthranilate synthase gene of pseudomonas syringae subsp. savastanoi. | the trpe gene, which encodes the large component of the enzyme anthranilate synthase, was isolated from a pseudomonas syringae subsp. savastanoi (p. savastanoi) cosmid library. cosmids that complemented an escherichia coli trpe mutation contained a gene whose product is 86% homologous at the deduced amino acid level to trpe of pseudomonas aeruginosa and pseudomonas putida. amino acid sequence comparison with other trpe sequences revealed the existence of conserved regions between the procaryotic ... | 1991 | 1987141 |
| cloning and characterization of an amidase gene from rhodococcus species n-774 and its expression in escherichia coli. | for investigation of an unknown open reading frame which is present upstream of the nitrile hydratase (nhase) gene from rhodococcus sp. n-774, a longer dna fragment covering the entire gene was cloned in escherichia coli. nucleotide sequencing and detailed subcloning experiments predicted a single open reading frame consisting of 521 amino acid residues of mr 54,671. the amino acid sequence, especially its nh2-terminal portion, showed significant homology with those of indoleacetamide hydrolases ... | 1991 | 2001397 |
| inactivation of auxin in tobacco transformed with the indoleacetic acid-lysine synthetase gene of pseudomonas savastanoi. | the iaal gene of pseudomonas syringae, subspecies savastanoi, encodes an indoleacetic acid (iaa)-lysine synthetase. to determine the effects of converting iaa to iaa-lysine in whole plants, the iaal gene was fused to a constitutive plant promoter and introduced into tobacco plants. biochemical analyses show that endogenous iaa is reduced by up to 19-fold in iaal plants. tobacco plants expressing the iaal gene display reduced apical dominance, reduced rooting, and inhibition of vascular different ... | 1991 | 2001843 |
| cloning vectors, derived from a naturally occurring plasmid of pseudomonas savastanoi, specifically tailored for genetic manipulations in pseudomonas. | a minimal replicon of 1.8 kb isolated from a 10-kb plasmid of pseudomonas savastanoi, pps10, has been used to obtain a collection of small vectors specific for pseudomonas (p. savastanoi, p. aeruginosa and p.putida). in addition, shuttle vectors that can be established both in pseudomonas and escherichia coli have been constructed by adding a pmb9 replicon. the vectors permit cloning of dna fragments generated by a variety of restriction enzymes using different antibiotic resistance markers for ... | 1990 | 2110095 |
| indoleacetic acid operon of pseudomonas syringae subsp. savastanoi: transcription analysis and promoter identification. | expression of the indoleacetic acid (iaa) operon, which contributes to the virulence of the phytopathogenic bacterium pseudomonas syringae subsp. savastanoi, was monitored by using broad-host-range lacz reporter gene plasmids. a combination of translational (gene) fusions and transcriptional (operon) fusions of p. syringae subsp. savastanoi sequences to lacz allowed localization of the iaa operon promoter. rna recovered from p. syringae subsp. savastanoi strains was mapped with iaa operon-specif ... | 1990 | 2120185 |
| characterization of is476 and its role in bacterial spot disease of tomato and pepper. | is476 is an endogenous insertion sequence present in copper-tolerant strains of xanthomonas campestris pv. vesicatoria. sequence analysis has revealed that the element is 1,225 base pairs in length, has 26-base-pair inverted repeats, and causes a 4-base-pair target site duplication upon insertion into the avirulence gene avrbs1. comparison of the full-length sequence with sequences in the national biomedical research foundation and national institutes of health data bases showed that one of the ... | 1990 | 2152895 |
| inhibition by agrobacterium tumefaciens and pseudomonas savastanoi of development of the hypersensitive response elicited by pseudomonas syringae pv. phaseolicola. | injection into tobacco leaves of biotype 1 agrobacterium tumefaciens or of pseudomonas savastanoi inhibited the development of a visible hypersensitive response to the subsequent injection at the same site of pseudomonas syringae pv. phaseolicola. this interference with the hypersensitive response was not seen with injection of bacterial growth medium or escherichia coli cells. live a. tumefaciens cells were required for the inhibitory effect. various mutants and strains of a. tumefaciens were e ... | 1990 | 2211508 |
| expression and fine structure of the gene encoding n epsilon-(indole-3-acetyl)-l-lysine synthetase from pseudomonas savastanoi. | the gene encoding n epsilon-(indole-3-acetyl)-l-lysine synthetase, iaal, from pseudomonas savastanoi was localized within a 4.25-kilobase ecori fragment derived from piaa1 of oleander strain ew 2009. two open reading frames of 606 and 1188 nucleotides were identified upon sequencing, which directed the in vitro synthesis of mr 21,000 and mr 44,000 proteins. expression of an open reading frame-2 subclone, pmon686, in escherichia coli indicates that (indole-3-acetyl)-l-lysine synthetase is encoded ... | 1990 | 2377619 |
| cotranscription of genes encoding indoleacetic acid production in pseudomonas syringae subsp. savastanoi. | indoleacetic acid (iaa) production by the plant pathogen pseudomonas syringae subsp. savastanoi is essential for tumor formation on olive and oleander. the bacterium produces iaa from tryptophan in reactions catalyzed by tryptophan monooxygenase and indoleacetamide hydrolase. the genetic determinants are, respectively, iaam and iaah. in oleander isolates, the genes encoding the iaa biosynthetic enzymes are located on a plasmid; in olive isolates, the genes occur on the chromosome. the iaa genes ... | 1989 | 2644217 |
| similarities between nucleotide sequences of insertion elements of agrobacterium tumefaciens and pseudomonas savastanoi in relation to agrobacterium tumefaciens tc-dna. | | 1987 | 2827126 |
| cloning of the gene for indoleacetic acid-lysine synthetase from pseudomonas syringae subsp. savastanoi. | the phytopathogen pseudomonas syringae subsp. savastanoi incites the production of galls on olive and oleander plants. gall formation is dependent on bacterial production of the phytohormone indoleacetic acid (iaa). the genetic determinants for iaa synthesis are located on a plasmid (piaa) and are organized in an operon in oleander strains of the bacterium. p. syringae subsp. savastanoi further converts iaa to an amino acid conjugate, 3-indole-acetyl-epsilon-l-lysine (iaa-lysine). the gene for i ... | 1986 | 3084452 |
| role of indoleacetic acid-lysine synthetase in regulation of indoleacetic acid pool size and virulence of pseudomonas syringae subsp. savastanoi. | the phytopathogen pseudomonas syringae subsp. savastanoi incites the production of galls on olive and oleander plants. gall formation is dependent upon the bacterial synthesis of the phytohormone indole-3-acetic acid (iaa). strains isolated from oleander galls are capable of further metabolizing iaa to an amino acid conjugate, 3-indoleacetyl-epsilon-l-lysine (iaa-lysine); bacterial olive gall isolates lack this activity. in this study, the cloned gene for iaa-lysine synthetase (iaal+) was introd ... | 1988 | 3129408 |
| minimal region necessary for autonomous replication of ptar. | the native 44-kilobase-pair plasmid ptar, discovered in a grapevine strain of agrobacterium tumefaciens, contains a single origin of dna replication confined to a 1.0-kilobase-pair region of the macromolecule. this region (ori) confers functions sufficient for replication in agrobacterium and rhizobium species but not in pseudomonas solanacearum, pseudomonas glumae, pseudomonas syringae pv. savastanoi, xanthomonas campestris pv. campestris, and escherichia coli. ori contains a repa gene that enc ... | 1988 | 3290199 |
| nucleotide sequence and expression of a pseudomonas savastanoi cytokinin biosynthetic gene: homology with agrobacterium tumefaciens tmr and tzs loci. | the nucleotide sequence of a pseudomonas trans-zeatin producing gene (ptz) from the pck1 plasmid of pseudomonas syringae pv. savastanoi strain 1006 has been determined. this gene confers upon e. coli the ability to synthesize and secrete several cytokinins including trans-zeatin, iso-pentenyladenine and their respective n9-ribosyl derivatives. sequence analysis indicates an open reading frame encoding a protein of 234 amino acids with a molecular weight of 26,816. significant sequence homology i ... | 1986 | 3515320 |
| expression of rhizobium meliloti nod genes in rhizobium and agrobacterium backgrounds. | rhizobium meliloti nod genes are required for the infection of alfalfa. induction of the nodc gene depends on a chemical signal from alfalfa and on nodd gene expression. by using a nodc-lacz fusion, we have shown that the induction of the r. meliloti nodc gene and the expression of nodd occur at almost normal levels in other rhizobium backgrounds and in agrobacterium tumefaciens, but not in escherichia coli. xanthomonas campestris, or pseudomonas savastanoi. our results suggest that bacterial ge ... | 1987 | 3597319 |
| cytokinin production by agrobacterium and pseudomonas spp. | the production of cytokinins by plant-associated bacteria was examined by radioimmunoassay. strains producing trans-zeatin were identified in the genera agrobacterium and pseudomonas. agrobacterium tumefaciens strains containing nopaline tumor-inducing plasmids, a. tumefaciens lippia isolates, and agrobacterium rhizogenes strains produced trans-zeatin in culture at 0.5 to 44 micrograms/liter. pseudomonas solanacearum and pseudomonas syringae pv. savastanoi produced trans-zeatin at levels of up t ... | 1987 | 3624204 |
| regulation of 3-indoleacetic acid production in pseudomonas syringae pv. savastanoi. purification and properties of tryptophan 2-monooxygenase. | the oxidative decarboxylation of l-tryptophan to yield 3-indoleacetamide, catalyzed by tryptophan 2-monooxygenase, represents a controlling reaction in the synthesis of indoleacetic acid by pseudomonas savastanoi (pseudomonas syringae pv. savastanoi), a gall-forming pathogen of olive (olea europea l.) and oleander (nerium oleander l.). production of indoleacetic acid is essential for virulence of the bacterium in its hosts. tryptophan 2-monooxygenase was characterized to determine its role in in ... | 1985 | 3997822 |
| tryptophan and indoleacetic acid transport in the olive and oleander knot organism pseudomonas savastanoi (e.f. smith) stevens. | | 1972 | 5078558 |
| [in vitro effect of different antibiotics and chemical substances on 90 strains of pseudomonas savastanoi]. | | 1968 | 5719705 |
| microbial synthesis and degradation of indole-3-acetic acid. i. the conversion of l-tryptophan to indole-3-acetamide by an enzyme system from pseudomonas savastanoi. | | 1966 | 5916389 |
| cloning characterization of iaam, a virulence determinant of pseudomonas savastanoi. | genes for indoleacetic acid production (iaam and iaah) are necessary for gall induction by the olive pathogen pseudomonas savastanoi. in strain 2009 these determinants are borne on plasmid piaa1. to map and characterize the genes, fragments of piaa1 generated by ecori endonuclease treatment were cloned in escherichia coli by using plasmid rsf1010 as vector. we isolated a recombinant plasmid encoding iaam, the locus for tryptophan 2-monooxygenase. this plasmid, called pluc1, was characterized by ... | 1982 | 6274847 |
| transposable element that causes mutations in a plant pathogenic pseudomonas sp. | a 1.3-kilobase-pair dna element, is51, causes a loss of virulence in the plant pathogen pseudomonas syringae pv. savastanoi. this sequence, is51, was first discovered in a plasmid-borne iaam locus, which together with iaah directs the synthesis of a virulence factor, indoleacetic acid. the spontaneous insertion of is51 in iaam resulted in the loss of indoleacetic acid production, attenuation of virulence, and the loss of both enzyme activities coded by iaam and iaah. using a cloned is51 element ... | 1983 | 6304006 |
| variability among isolates of pseudomonas syringae pv. savastanoi from the phylloplane of the olive. | leaves of three or four different ages were taken from olive plants quarterly in 1974-1980. one thousand and fifty isolates of pseudomonas syringae pv. savastanoi from the phylloplane were tested for virulence to the olive and subjected to numerical phenetic analysis using 60 unit characters. the data were analysed using unweighted average linkage (upgma) and single linkage clustering on the simple matching (ssm) and pattern (sp) coefficients. the isolates obtained from leaves of a given age at ... | 1983 | 6886676 |
| involvement of plasmid deoxyribonucleic acid in indoleacetic acid synthesis in pseudomonas savastanoi. | olive (or oleander) knot is a plant disease incited by pseudomonas savastanoi. disease symptoms consist of tumorous outgrowths induced in the plant by bacterial production of indole-3-acetic acid (iaa). synthesis of iaa occurs by the following reactions: l-tryptophan leads to indoleacetamide leads to indoleacetic acid, catalyzed by tryptophan 2-monooxygenase and indoleacetamide hydrolase, respectively. whereas the enzymology of iaa synthesis is well characterized, nothing is known about the gene ... | 1980 | 7204339 |
| the nodd locus from azorhizobium caulinodans is flanked by two repetitive elements. | the sequence surrounding the azorhizobium caulinodans (ac) regulatory nodd gene was analyzed. upstream from nodd and in the opposite orientation, two small open reading frames were identified (orf1 and orf2). the dna sequence corresponding to orf1, termed epsilon 1, is similar to a part of the insertion element is51 from pseudomonas savastanoi. immediately downstream from nodd, a repeated element, delta 1, has been described [goethals et al., mol. plant-microbe interact. 5 (1992) 405-411]. the e ... | 1995 | 7590297 |
| purification and characterization of the flavoprotein tryptophan 2-monooxygenase expressed at high levels in escherichia coli. | tryptophan 2-monooxygenase from pseudomonas savastanoi is a flavoprotein which catalyzes the formation of indoleacetamide from tryptophan. this is the first step in a two-step pathway for the formation of indoleacetic acid during infection of plants and subsequent gall formation by this and other bacteria. the enzyme has been expressed in escherichia coli at high levels, and a purification procedure has been developed which generates micromolar amounts of protein. the purified enzyme contains ti ... | 1995 | 7840624 |
| characterization of high-frequency deletions in the iaa-containing plasmid, piaa2, of pseudomonas syringae pv. savastanoi. | the phytopathogenic bacterium pseudomonas syringae pv. savastanoi causes olive and oleander knot disease. the bacterium induces the formation of tumorous galls by the synthesis and secretion of the plant hormones trans-zeatin riboside and indole-3-acetic acid into host intercellular spaces. an italian oleander isolate, pb213, has been observed to lose the ability to synthesize iaa at high frequency, thus becoming non-pathogenic. the iaa genes, located on the 72-kb iaa-containing plasmid, piaa2 w ... | 1994 | 7909615 |
| amidase coupled with low-molecular-mass nitrile hydratase from rhodococcus rhodochrous j1. sequencing and expression of the gene and purification and characterization of the gene product. | the cloned 9.4-kb insert of plasmid pnhj20l containing low-molecular-mass nitrile hydratase (l-nhase) gene from rhodococcus rhodochrous j1 [kobayashi, m. et al. (1991) biochim. biophys. acta 1129, 23-33] was digested with various restriction enzymes, and the trimmed fragments were inserted into puc18 or puc19. a 1.96-kb ecori-sphi region located 1.9-kb downstream of the l-nhase gene was found to be essential for the expression of amidase activity in escherichia coli; the gene arrangement of the ... | 1993 | 7916690 |
| a mutation in the indole-3-acetic acid biosynthesis pathway of pseudomonas syringae pv. syringae affects growth in phaseolus vulgaris and syringomycin production. | homologs of the genes for indole-3-acetic acid (iaa) biosynthesis from pseudomonas syringae pv. savastanoi were retrieved from a genomic library of p. syringae pv. syringae, and their nucleotide sequences were determined. sequence relatedness between the p. syringae pv. syringae and p. syringae pv. savastanoi iaa operons is greater than 90% within the iaam and iaah loci but declines dramatically at a position approximately 200 bp 5' of the iaam translation initiation codon. a third open reading ... | 1994 | 8113177 |
| the 73-kb piaa plasmid increases competitive fitness of pseudomonas syringae subspecies savastanoi in oleander. | pseudomonas syringae subsp. savastanoi causes tumors on olive and oleander by producing the plant growth regulators indoleacetic acid (iaa) and cytokinins following infection of the plant. the contribution of iaa production to the ability of p. syringae subsp. savastanoi to grow and survive in oleander leaf tissue was studied. bacterial strains differing only with respect to iaa production were characterized. growth and survival of wild-type and two mutant strains of p. syringae subsp. savastano ... | 1993 | 8364801 |
| characterization of an insertion sequence (is53) located within is51 on the iaa-containing plasmid of pseudomonas syringae pv. savastanoi. | a 2568-base pair (bp) insertion sequence (is53) was cloned from the iaa-containing plasmid piaa2 of pseudomonas syringae subsp. savastanoi. is53 is located downstream from the iaa operon and is inserted into is51, a previously described element on piaa2. is53 causes an 8-bp target duplication and has nearly perfect terminal inverted repeats of 27 bp. several degenerate copies of the inverted repeats are located internal, and in direct orientation to the terminal inverted repeats. a putative tran ... | 1993 | 8385786 |
| analysis of cumene (isopropylbenzene) degradation genes from pseudomonas fluorescens ip01. | we obtained the dna fragments encoding 2-hydroxy-6-oxo-7-methylocta-2,4-dienoic acid (homoda) hydrolase in the cumene (isopropylbenzene) degrader pseudomonas fluorescens strain ip01 via pcr using two synthesized oligonucleotides corresponding to the conserved regions within known meta-cleavage compound hydrolases. following colony hybridization using the amplified dna as a probe, a 4.5-kb hindiii fragment was isolated from p. fluorescens ip01. after determining the nucleotide sequence of this fr ... | 1996 | 8953719 |
| denitration of 2,4,6-trinitrotoluene by pseudomonas savastanoi. | past disposal of wastewaters containing 2,4,6-trinitrotoluene (tnt) at the former nebraska ordnance plant has resulted in numerous acres of tnt-contaminated soil. examining the microbial population of these soils revealed several tnt-tolerant pseudomonas spp. we selected one species, p. savastanoi, to determine its ability to transform tnt. pure culture experiments were performed in pseudomonas minimal medium containing 0.31 mm tnt (70 mg tnt . l(-1)) under varied nutrient and cell density regim ... | 1997 | 9198535 |
| genetic engineering of parthenocarpic plants. | transgenic tobacco and eggplants expressing the coding region of the iaam gene from pseudomonas syringae pv. savastanoi, under the control of the regulatory sequences of the ovule-specific defh9 gene from antirrhinum majus, showed parthenocarpic fruit development. expression of the defh9-iaam chimeric transgene occurs during flower development in both tobacco and eggplant. seedless fruits were produced by emasculated flowers. when pollinated, the parthenocarpic plants produced fruits containing ... | 1997 | 9415894 |
| dna relatedness among the pathovars of pseudomonas syringae and description of pseudomonas tremae sp. nov. and pseudomonas cannabina sp. nov. (ex sutic and dowson 1959). | a total of 48 pathovars of pseudomonas syringae and eight related species were studied by dna-dna hybridization (s1 nuclease method) and ribotyping. the existence of nine discrete genomospecies was indicated. genomospecies 1 corresponded to p. syringae sensu stricto and included p. syringae pathovars syringae, aptata, lapsa, papulans, pisi, atrofaciens, aceris, panici, dysoxyli and japonica. genomospecies 2 included p. syringae pathovars phaseolicola, ulmi, mori, lachrymans, sesami, tabaci, mors ... | 1999 | 10319466 |
| molecular analysis of a tryptophan-2-monooxygenase gene (iaam) of agrobacterium vitis. | tryptophan-2-monooxygenase genes occur in a number of bacteria and encode the conversion of tryptophan to the plant hormone precursor indole-3-acetamide. the role of these genes in the plant-bacteria interaction is often unclear. however, their function as a virulence determinant is established for pseudomonas savastanoi and agrobacterium tumefaciens. some members of the agrobacteria, such as agrobacterium vitis have a limited host range. we have characterized the tryptophan-2-monooxygenase (iaa ... | 1999 | 10727091 |
| detection of pseudomonas savastanoi pv. savastanoi in olive plants by enrichment and pcr. | the sequence of the gene iaal of pseudomonas savastanoi ew2009 was used to design primers for pcr amplification. the iaal-derived primers directed the amplification of a 454-bp fragment from genomic dna isolated from 70 strains of p. savastanoi, whereas genomic dna from 93 non-p. savastanoi isolates did not yield this amplified product. a previous bacterial enrichment in the semiselective liquid medium pvf-1 improved the pcr sensitivity level, allowing detection of 10 to 100 cfu/ml of plant extr ... | 2000 | 10831456 |
| analysis of the roles of amino acid residues in the flavoprotein tryptophan 2-monooxygenase modified by 2-oxo-3-pentynoate: characterization of his338, cys339, and cys511 mutant enzymes. | the flavoprotein tryptophan 2-monooxygenase catalyzes the oxidative decarboxylation of tryptophan to indoleacetamide. his338, cys339, and cys511 of the pseudomonas savastanoi enzyme were previously identified as possible active-site residues by modification with 2-oxo-3-pentynoate ([g. gadda, l.j. dangott, w.h. johnson jr., c.p. whitman, p.f. fitzpatrick, biochemistry 38 (1999) 5822-5828]). the h338n, c339a, and c511s enzymes have been characterized to determine the roles of these residues in ca ... | 2002 | 12051679 |
| modulation of pps10 host range by dnaa. | narrow-host-range plasmid pps10, originally found in pseudomonas savastanoi, is unable to replicate in other strains such as escherichia coli. here, we report that the establishment of pps10 in e. coli can be achieved by a triple mutation in the dnaa gene of e. coli (dnaa403), leading to q14amber, p297s and a412v changes in the dnaa host replication protein (dnaa403 mutant). as the e. coli strain used contained double amber suppressor mutations (supe, supf), the amber codon in dnaa403 can be tra ... | 2002 | 12366845 |
| highly sensitive detection of pseudomonas savastanoi pv. savastanoi in asymptomatic olive plants by nested-pcr in a single closed tube. | a nested-polymerase chain reaction (pcr) has been set up to be performed in a single closed tube for the detection of pseudomonas savastanoi pv. savastanoi. nested-pcr coupled with dot-blot hybridization was able to detect up to one cell of the target per ml of olive extract, showing the greatest sensitivity compared with all previously reported detection assays. validation of the developed procedure for diagnosis and epidemiological purposes was achieved by testing ca. 240 asymptomatic plant sa ... | 2003 | 12459247 |
| [vascular bundle specific expression of iaal gene affects the generation frequencies of transgenic tobacco]. | a vascular bundles specific expressing vector pbal1 with a promoter aq630 from rice phenylalanine ammonialyase gene and a gene encoding indoleacetic-lysine synthytase from pseudomonas syringae subsp. savastanoi was constructed. affirmed by southern blotting and rtpcr analysis, the aq630-iaal transgenic plants show increasing shoots-regeneration frequency of young stem explants on hormone-free 1/2 ms medium and lower sensibility to iaa when roots were induced from the root explants on the media c ... | 1999 | 12548796 |
| modulation of pps10 host range by plasmid-encoded repa initiator protein. | we report here the isolation and analysis of novel repa host range mutants of pps10, a plasmid originally found in pseudomonas savastanoi. upon hydroxylamine treatment, five plasmid mutants were selected for their establishment in escherichia coli at 37 degrees c, a temperature at which the wild-type form cannot be established. the mutations were located in different functional regions of the plasmid repa initiation protein, and the mutants differ in their stable maintenance, copy number, and ab ... | 2003 | 12562807 |
| [electron microscopy of several strains of pseudomonas savastanoi]. | | 1956 | 13348092 |
| [not available]. | | 1956 | 13418354 |
| [new technic for the identification of pseudomonas savastanoi]. | | 1956 | 13430377 |
| [study of the hormonal fraction of tumors produced in olives by pseudomonas savastanoi. ii. chemical and biological evaluation of beta-indolacetic acid]. | | 1963 | 13967223 |
| [study of the hormonal fraction of the tumors produced in the olive tree by pseudomonas savastanoi. i. biogenesis of the beta-indolacetic acid content of the tumors]. | | 1962 | 13967224 |
| indoleacetamide as an intermediate in the synthesis of indoleacetic acid in pseudomonas savastanoi. | when dl-tryptophan-2-c(14) was incubated with washed cells or cell-free preparations of pseudomonas savastanoi, two radioactive metabolites were formed. one was identified as indoleacetamide and the second, indoleacetic acid. the amount of indoleacetamide in the reaction mixture increased rapidly during the early stages of incubation; it reached a peak after 15 minutes and declined steadily there-after. indoleacetic acid, on the other hand, accumulated slowly throughout the incubation period. ce ... | 1963 | 14059783 |
| [metabolism of tryptophan by pseudomonas savastanoi]. | | 1964 | 14335559 |
| erwinia toletana sp. nov., associated with pseudomonas savastanoi-induced tree knots. | gram-negative bacteria were isolated from knots induced by pseudomonas savastanoi in olive trees (olea europaea l.). a total of nine endophytic bacterial strains were isolated, each from inside a different tree knot. biochemical characterization indicated that all the strains belong to the family enterobacteriaceae. phylogenetic analyses of the 16s rrna genes of these novel isolates revealed that they formed a homogeneous cluster within erwinia species. dna signatures of these isolates were iden ... | 2004 | 15545461 |
| kinetic analysis of the l-ornithine transcarbamoylase from pseudomonas savastanoi pv. phaseolicola that is resistant to the transition state analogue (r)-n delta-(n'-sulfodiaminophosphinyl)-l-ornithine. | (r)-n(delta)-(n'-sulfodiaminophosphinyl)-l-ornithine (psorn) is the active component of a phytotoxin, called phaseolotoxin, produced by pseudomonas savastanoi pv. phaseolicola. psorn acts as a potent transition state (ts) inhibitor of ornithine transcarbamoylase (otcase, e.c. 2.1.3.3) that binds to the otcase from escherichia coli (argi) with a dissociation constant of 1.6 pm. while inhibition of otcase can lead to arginine auxotrophy, p. savastanoi pv. phaseolicola is able to synthesize toxin w ... | 2005 | 15766270 |
| 'candidatus erwinia dacicola', a coevolved symbiotic bacterium of the olive fly bactrocera oleae (gmelin). | the taxonomic identity of the hereditary prokaryotic symbiont of the olive fly bactrocera oleae (diptera: tephritidae) was investigated. in order to avoid superficial microbial contaminants and loosely associated saprophytic biota, flies were surface-sterilized at the larval stage and reared under aseptic conditions until adult emergence. b. oleae flies originating from different geographical locations and collected at different times of the year were tested. bacterial isolation was undertaken f ... | 2005 | 16014495 |
| identification and plant interaction of a phyllobacterium sp., a predominant rhizobacterium of young sugar beet plants. | the second most abundant bacterium on the root surface of young sugar beet plants was identified as a phyllobacterium sp. (rhizobiaceae) based on a comparison of the results of 39 conventional identification tests, 167 api tests, 30 antibiotic susceptibility tests, and sodium dodecyl sulfate-polyacrylamide gel electrophoretic fingerprints of total cellular proteins with type strains of phyllobacterium myrsinacearum and phyllobacterium rubiacearum. it was found on 198 of 1,100 investigated plants ... | 1990 | 16348158 |
| rapid in situ assay for indoleacetic acid production by bacteria immobilized on a nitrocellulose membrane. | we have developed a new assay that differentiates between indoleacetic acid (iaa)-producing and -nonproducing bacteria on a colony plate lift. medium supplemented with 5 mm l-tryptophan is inoculated with isolates of interest, overlaid with a nitrocellulose membrane, and then incubated until bacterial colonies reach 1 to 2 mm in diameter. the membrane is removed to a filter paper saturated with salkowski reagent and incubated until distinct red haloes form around the colonies. the colorimetric r ... | 1991 | 16348419 |
| chemotaxis of pseudomonas syringae subsp. savastanoi virulence mutants. | several mutants of pseudomonas syringae subsp. savastanoi were tested for their ability to sense and respond to a chemotactic gradient in low concentrations of yeast extract. the mutants were deficient in one or both of the genes coding for the synthesis of the plant hormones indole-3-acetic acid (iaa) and isopentenyl adenosine. mutations which resulted in the loss of iaa production were due to the loss of the entire plasmid containing the iaa operon or to an 18-kb deletion of the iaa region. ad ... | 1991 | 16348565 |
| [fatty acids composition of cellular lipids of the collected and newly isolated pseudomonas lupini strains]. | fatty acid composition of cellular lipids of 23 pseudomonas lupini strains (beltjukova et koroljova 1968) has been investigated. cellular fatty acids which contained from c10 to c19 carbon atoms have been identified. basic fatty acid of those pseudomonas cells are hexadecanoic, hexadecenoic and octadecanoic acids. the 3-hydroxydecanoic (c10:0 3oh), 3-hydroxydodecanoic (c12:0 3oh), 2-hydroxydodecanoic (c12:0 2oh) and cyclopropane fatty acids which contain 17 and 19 carbon atoms have been detected ... | 2005 | 16396109 |
| nucleotide sequences of the pseudomonas savastanoi indoleacetic acid genes show homology with agrobacterium tumefaciens t-dna. | we report the nucleotide sequences of iaam and iaah, the genetic determinants for, respectively, tryptophan 2-monooxygenase and indoleacetamide hydrolase, the enzymes that catalyze the conversion of l-tryptophan to indoleacetic acid in the tumor-forming bacterium pseudomonas syringae pv. savastanoi. the sequence analysis indicates that the iaam locus contains an open reading frame encoding 557 amino acids that would comprise a protein with a molecular weight of 61,783; the iaah locus contains an ... | 1985 | 16593610 |
| insertion sequence elements of pseudomonas savastanoi: nucleotide sequence and homology with agrobacterium tumefaciens transfer dna. | two types of transposable elements, is51 and is52 (is, insertion sequence), were found in pseudomonas syringae subsp. savastanoi (p. savastanoi) that spontaneously insert into and inactivate iaam; the insertion results in the loss of indoleacetic acid production and attenuation of virulence. the nucleotide sequences of both is elements have sizes and structural features common to other prokaryotic is elements; is51 is 1311 base pairs (bp) long and has terminal inverted repeats of 26 bp; is52 is ... | 1986 | 16593778 |
| secretion of zeatin, ribosylzeatin, and ribosyl-1'' -methylzeatin by pseudomonas savastanoi: plasmid-coded cytokinin biosynthesis. | cytokinin production by strains of the phytopathogenic bacterium pseudomonas syringae pv savastanoi was measured by immunoaffinity chromatography of the culture medium on immobilized anti-cytokinin antibodies, followed by high performance liquid chromatography, radioimmunoassay and mass spectrometry. p. savastanoi strain pb213-2 secretes zeatin (80 nanograms per milliliter) and ribosylzeatin (80 nanograms per milliliter). even higher levels of zeatin (400 nanograms per milliliter) are produced b ... | 1986 | 16665104 |
| evidence for cytokinin involvement in rhizobium (ic3342)-induced leaf curl syndrome of pigeonpea (cajanus cajan millsp.). | a uniquely abnormal shoot development (shoot tip-bending, leaf curling, release from apical dominance, and stunted growth) in pigeonpea (cajanus cajan millsp) induced by a nodulating rhizobium strain, ic3342, is thought to be due to a hormonal imbalance. amaranthus betacyanin bioassay indicated that xylem exudate and leaf extracts from pigeonpea plants with rhizobium-induced leaf curl symptoms contained high concentrations of cytokinin relative to those in normal plants. radioimmunoassay (ria) o ... | 1991 | 16668086 |
| rapid identification of cytokinins by an immunological method. | a method for rapid identification of bacterial cytokinins has been developed in which cultures are fed [(3)h]adenine, the cytokinins (including (3)h-labeled cytokinins) are isolated by immunoaffinity chromatography, and analyzed by hplc with on-line scintillation counting. analysis of agrobacterium tumefaciens strains showed that some produced primarily trans-zeatin, whereas others produced primarily trans-zeatin riboside. pseudomonas syringae pv savastanoi produced mixtures of trans-zeatin, dih ... | 1991 | 16668105 |
| auxin flow in anther filaments is critical for pollen grain development through regulating pollen mitosis. | it was well known that auxin is critical for anther/pollen grain development, however, the clear distribution and detailed effects of auxin during floral development are still unclear. we have shown here that, through analyzing gus activities of arabidopsis lines harboring auxin response elements dr5-gus, auxin was mainly accumulated in the anther during flower stages 10-12. further studies employing the indoleacetic acid-lysine synthetase (iaal) coding gene from pseudomonas syringae subsp. sava ... | 2006 | 16786302 |
| changes in phenolic composition induced by pseudomonas savastanoi pv. savastanoi infection in olive tree: presence of large amounts of verbascoside in nodules of tuberculosis disease. | a study was carried out to determine the changes in phenolic composition induced by tuberculosis infection in olive trees. four ethanolic extracts were compared: olive leaf from shoots affected by pseudomonas savastanoi pv. savastanoi, nodules induced by this bacteria, leaf from healthy (asymptomatic) shoots, and shoots. among the differences found, the presence of a phenolic compound in nodules was significant in much larger quantities than in leaf or shoots. mass spectrometric analysis showed ... | 2006 | 16848518 |
| biofilm formation and cellulose expression among diverse environmental pseudomonas isolates. | the ability to form biofilms is seen as an increasingly important colonization strategy among both pathogenic and environmental bacteria. a survey of 185 plant-associated, phytopathogenic, soil and river pseudomonas isolates resulted in 76% producing biofilms at the air-liquid (a-l) interface after selection in static microcosms. considerable variation in biofilm phenotype was observed, including waxy aggregations, viscous and floccular masses, and physically cohesive biofilms with continuously ... | 2006 | 17014498 |
| strain selection and improvement of gene transfer for genetic manipulation of pseudomonas savastanoi isolated from olive knots. | research on diseases of herbaceous plants caused by pseudomonads has been rapidly progressing; however, for most pathovars which infect woody plants, strains accessible to genetic manipulation have not yet been reported. at present, few studies have reported the transfer of genes to pseudomonas savastanoi pv. savastanoi, the causal agent of olive knot disease. a collection of p. savastanoi pv. savastanoi isolates was tested for its ability to receive, by conjugation, the broad-host range plasmid ... | 2007 | 17113758 |
| mechanistic studies of the flavoenzyme tryptophan 2-monooxygenase: deuterium and 15n kinetic isotope effects on alanine oxidation by an l-amino acid oxidase. | tryptophan 2-monooxygenase (tmo) from pseudomonas savastanoi catalyzes the oxidative decarboxylation of l-tryptophan during the biosynthesis of indoleacetic acid. structurally and mechanistically, the enzyme is a member of the family of l-amino acid oxidases. deuterium and 15n kinetic isotope effects were used to probe the chemical mechanism of l-alanine oxidation by tmo. the primary deuterium kinetic isotope effect was ph independent over the ph range 6.5-10, with an average value of 6.0 +/- 0. ... | 2006 | 17176107 |
| [phenotypical and genotypical characteristics of the pathogen in lupine bacterial brown spottiness]. | it has been determined with the help of analyzing the complex of phenotypical and genotypical properties, that pseudomonas lupini is not a separate species. it has been shown that lupin's bacterial spot is induced by phytopathogenic bacteria of two species p. syringae and p. savastanoi. on the basis of their high phenotypical and genotypical similarity with a typical strain pseudomonas syringae pv. syringae b1027 the majority of investigated strains are related to p. syringae. the only exception ... | 2006 | 17243363 |
| recovery of pseudomonas savastanoi pv. savastanoi from symptomless shoots of naturally infected olive trees. | seasonal dynamics of pseudomonas savastanoi pv. savastanoi (psv) on stems and leaves from symptomless shoots of naturally infected olive trees was monitored in spanish olive orchards. data inferred from the comparison between washing of leaves and dilution-plating versus leaf printing of individual leaves suggested that psv population sizes varied by over several orders of magnitude, among leaves sampled concurrently from the same shoot. we did not find significant differences between leaves and ... | 2007 | 17661284 |
| global genomic analysis of pseudomonas savastanoi pv. savastanoi plasmids. | pseudomonas savastanoi pv. savastanoi strains harbor native plasmids belonging to the ppt23a plasmid family (pfps) which are detected in all pathovars of the related species pseudomonas syringae examined and contribute to the ecological and pathogenic fitness of their host. however, there is a general lack of information about the gene content of p. savastanoi pv. savastanoi plasmids and their role in the interaction of this pathogen with olive plants. we designed a dna macroarray containing 135 ... | 2008 | 17993520 |
| separation of plant pathogens from different hosts and tissues by capillary electromigration techniques. | in this contribution capillary isoelectric focusing and capillary zone electrophoresis were applied for the separation and detection of different plant pathogens including pseudomonas syringae pv. syringae, p. syringae pv. lachrymans, pseudomonas savastanoi pv. fraxinus, p. savastanoi pv. olea, agrobacterium tumefaciens, a vitis, xanthomonas arboricola pv. juglandis, x. campestris pv. zinniae, and curtobacterium sp.. the uv detection and sensitive fluorescence detection of the native phytopathog ... | 2007 | 17997525 |
| the structure of the o-specific polysaccharide of the lipopolysaccharide from pantoea agglomerans strain fl1. | a neutral o-specific polysaccharide consisting of d-rhamnose was obtained by mild acid hydrolysis of the lipopolysaccharide of the plant pathogenic bacterium pantoea agglomerans strain fl1, a common epiphyte of many plant species, and associated with pseudomonas savastanoi pv. savastanoi in young and apparently intact olive knots. by means of compositional and methylation analyses, and nmr spectroscopy, the chemical repeating unit of the polymer was identified as a linear tetrasaccharide of the ... | 2008 | 18039532 |
| the influence of l-ascorbic acid on the antibacterial-toxic activity of aflatoxins on adsorbent layer. | to substantiate the role of formaldehyde (hcho) and its reaction products in the mechanism of the antibacterial-toxic effect of aflatoxins b1 (afb1), b2, g1 and g2. | 2007 | 18045436 |
| is53: an insertion element for molecular typing of pseudomonas savastanoi pv. savastanoi. | a worldwide collection of pseudomonas savastanoi pv. savastanoi strains from olive knots was examined for the distribution, variation in position and copy numbers of the is53 insertion element. southern hybridization analysis of plasmid dna from six olive strains using is53 and repa probes revealed that this insertion element was present in the chromosomal replicon and not in a plasmid, as had been originally described in an oleander strain. southern hybridization analysis also revealed that is5 ... | 2008 | 18359611 |
| introduction of a novel pathway for iaa biosynthesis to rhizobia alters vetch root nodule development. | we introduced into rhizobium leguminosarum bv. viciae lpr1105 a new pathway for the biosynthesis of the auxin, indole-3-acetic acid (iaa), under the control of a stationary phase-activated promoter active both in free-living bacteria and bacteroids. the newly introduced genes are the iaam gene from pseudomonas savastanoi and the tms2 gene from agrobacterium tumefaciens. free-living bacteria harbouring the promoter-iaamtms2 construct release into the growth medium 14-fold more iaa than the wild-t ... | 2008 | 18415080 |
| diversity of auxin-producing bacteria associated to pseudomonas savastanoi -induced olive knots. | forty three strains were isolated from knots induced by pseudomonas savastanoi in different olive cultivars. all the selected bacteria were shown to produce variable amounts of the plant growth hormone indole-3-acetic acid (iaa). amplification of the intergenic transcribed spacers (its) between 16s and 23s rdna genes, allowed the clustering of the isolates into seven distinct groups. all isolates from its group 1 were positive to the pseudomonas savastanoi pv. savastanoi specific iaa l gene as s ... | 2008 | 18759227 |
| effects of specific expression of iaal gene in tobacco tapetum on pollen embryogenesis. | the indoleacetic-acid-lysine synthetase (iaal) gene from pseudomonas syringae subsp. savastanoi was fused to tobacco tapetum-specific expression promoter ta29, and introduced into tobacco. the expression pattern of this chimeric gene was studied, and the endogenous indoleacetic acid (iaa) levels in different organs were assayed. the results demonstrated that ta29 promoter was only able to direct the specific expression of iaal gene in transgenic tobacco anther, and resulted in the decrease of en ... | 1997 | 18762878 |
| biocontrol of pseudomonas savastanoi, causative agent of olive knot disease: antagonistic potential of non-pathogenic rhizosphere isolates of fluorescent pseudomonas. | | 2008 | 18831274 |
| in vitro analysis of the interaction of pseudomonas savastanoi pvs. savastanoi and nerii with micropropagated olive plants. | this study assessed the use of in vitro olive plants to evaluate the virulence of pseudomonas savastanoi pv. savastanoi strains isolated from olive and p. savastanoi pv. nerii strains isolated from oleander knots. first, different olive isolates were inoculated into stem wounds and differences in knot formation and weight of overgrowths were observed for the selected strains. tissue proliferation was clearly visible in all inoculated plants 30 days after inoculation. virulence of p. savastanoi p ... | 2008 | 18943258 |
| knot formation caused by pseudomonas syringae subsp. savastanoi on olive plants is hrp-dependent. | abstract the virulence of pseudomonas syringae subsp. savastanoi, which causes hyperplastic symptoms (knots) on olive plants, is associated with secreted phytohormones. we identified a tn5-induced mutant of p. syringae subsp. savastanoi that did not cause disease symptoms on olive plants although it was still able to produce phytohormones. in addition, the mutant failed to elicit a hypersensitive response in a nonhost plant. molecular characterization of the mutant revealed that a single tn5 ins ... | 2004 | 18943767 |
| multiplex nested reverse transcription-polymerase chain reaction in a single tube for sensitive and simultaneous detection of four rna viruses and pseudomonas savastanoi pv. savastanoi in olive trees. | abstract a multiplex nested reverse transcription-polymerase chain reaction (rt-pcr) in a single closed tube was developed for the simultaneous detection of four rna viruses: cucumber mosaic virus, cherry leaf roll virus, strawberry latent ringspot virus, and arabis mosaic virus, and the bacterium pseudomonas savastanoi pv. savastanoi. the method enabled, for the first time, the sensitive and simultaneous detection of rna and dna targets from plant viruses and a bacterium, saving time, decreasin ... | 2003 | 18944338 |
| factors affecting pseudomonas savastanoi pv. savastanoi plant inoculations and their use for evaluation of olive cultivar susceptibility. | abstract pseudomonas savastanoi pv. savastanoi causes olive knot disease, which is present in most countries where olive trees are grown. although the use of cultivars with low susceptibility may be one of the most appropriate methods of disease control, little information is available from inoculation assays, and cultivar susceptibility assessments have been limited to few cultivars. we have evaluated the effects of pathogen virulence, plant age, the dose/response relationship, and the inductio ... | 2006 | 18944447 |
| potential role of formaldehyde in the mechanism of action of ascorbigens on the basis of bioarena studies. | the effect of ascorbigen and 1'-methylascorbigen as a model compound pair was studied on the phytopathogenic bacterium pseudomonas savastanoi pv. phaseolicola in the bioarena experimental system after overpressured layer chromatography. results showed a characteristic, strong antibacterial effect of 1'-methylascorbigen and weak effect of ascorbigen present on the adsorbent layer as chromatographic spot. addition of formaldehyde capture compounds (l-arginine, glutathione, dimedone) partially or t ... | 2009 | 19039806 |
| pseudomonas savastanoi pv. savastanoi contains two iaal paralogs, one of which exhibits a variable number of a trinucleotide (tac) tandem repeat. | in this study, pseudomonas savastanoi pv. savastanoi isolates were demonstrated to contain two iaal paralogs, which are both chromosomally located in most strains. comparative analysis of iaal nucleotide sequences amplified from these two paralogs revealed that one paralog, iaal(psn), is 100% identical to iaal from p. savastanoi pv. nerii, while the other paralog, iaal(psv), exhibited 93% identity to iaal from pseudomonas syringae pv. tomato (iaal(pto)). a 3-nucleotide motif (tac) comprised of 3 ... | 2009 | 19098222 |
| delineation of pseudomonas savastanoi pv. savastanoi strains isolated in tunisia by random-amplified polymorphic dna analysis. | to investigate the genetic diversity of pseudomonas savastanoi pv. savastanoi strains and to look whether these strains were distributed to geographical location. | 2009 | 19191961 |
| fate of a pseudomonas savastanoi pv. savastanoi type iii secretion system mutant in olive plants (olea europaea l.). | pseudomonas savastanoi pv. savastanoi strain ncppb 3335 is a model bacterial pathogen for studying the molecular basis of disease production in woody hosts. we report the sequencing of the hrps-to-hrpz region of ncppb 3335, which has allowed us to determine the phylogenetic position of this pathogen with respect to previously sequenced pseudomonas syringae hrp clusters. in addition, we constructed a mutant of ncppb 3335, termed t3, which carries a deletion from the 3' end of the hrps gene to the ... | 2010 | 20363790 |
| annotation and overview of the pseudomonas savastanoi pv. savastanoi ncppb 3335 draft genome reveals the virulence gene complement of a tumour-inducing pathogen of woody hosts. | pseudomonas savastanoi pv. savastanoi is a tumour-inducing pathogen of olea europaea l. causing olive knot disease. bioinformatic analysis of the draft genome sequence of strain ncppb 3335, which encodes 5232 predicted coding genes on a total length of 5856 998 bp and a 57.12% g + c, revealed a large degree of conservation with pseudomonas syringae pv. phaseolicola 1448a and p. syringae pv. tabaci 11528. however, ncppb 3335 contains twelve variable genomic regions, which are absent in all previo ... | 2010 | 20370821 |
| development of a versatile tool for the simultaneous differential detection of pseudomonas savastanoi pathovars by end point and real-time pcr. | pseudomonas savastanoi pv. savastanoi is the causal agent of olive knot disease. the strains isolated from oleander and ash belong to the pathovars nerii and fraxini, respectively. when artificially inoculated, pv. savastanoi causes disease also on ash, and pv. nerii attacks also olive and ash. surprisingly nothing is known yet about their distribution in nature on these hosts and if spontaneous cross-infections occur. on the other hand sanitary certification programs for olive plants, also incl ... | 2010 | 20509893 |
| an rhs-like genetic element is involved in bacteriocin production by pseudomonas savastanoi pv. savastanoi. | the main aim of this work was the identification of genetic determinants involved in bacteriocin production by strain itm317 of pseudomonas savastanoi pv. savastanoi, besides bacteriocin characterization. the bacteriocin was observed to be a heat-sensitive, high molecular weight proteinaceous compound. we identified a transposon (tn5)-induced mutant which had lost its ability to produce the bacteriocin. the tn5 insertion's responsibility for the above mutated phenotype was demonstrated by marker ... | 2010 | 20563849 |
| location and activity of members of a family of virppha homologues in pathovars of pseudomonas syringae and p. savastanoi. | summary virppha is a major determinant of the pathogenicity of pseudomonas savastanoi pv. phaseolicola to phaseolus bean. a family of homologues of virppha was detected in pathovars of p. savastanoi and p. syringae. we examined the structure and activity of alleles designated virppha, virppha(pgy), and virppha(psv) from p. savastanoi pathovars phaseolicola, glycinea, and savastanoi, respectively, and avrptob from p. syringae pv. tomato. sequencing showed that the virppha(pgy) homologue had a 48- ... | 2002 | 20569328 |
| culturable bacterial microflora associated with nectarine fruit and their potential for control of brown rot. | microflora of fruit surfaces have been the best source of antagonists against fungi causing postharvest decay of fruit. however, there is little information on microflora colonizing surfaces of fruits other than grape, apple, and citrus. we characterized bacterial microflora on nectarine fruit surfaces from the early stage of development until harvest. identification of bacterial strains was made using midi (fatty acid methyl ester analysis) and biolog systems. biolog identified 35% and midi 53% ... | 2010 | 20657618 |
| erwinia oleae sp. nov., isolated from olive knots caused by pseudomonas savastanoi pv. savastanoi. | three endophytic bacterial isolates were obtained in italy from olive knots caused by pseudomonas savastanoi pv. savastanoi. phenotypic tests in combination with 16s rrna gene sequence analysis indicated a phylogenetic position of these isolates in the genus erwinia or pantoea, and revealed two other strains with highly similar 16s rrna gene sequences (> 99 %), cect 5262 and cect 5264, obtained in spain from olive knots. rep-pcr dna fingerprinting of the five strains from olive knots with box, e ... | 2010 | 21186287 |
| endopathogenic lifestyle of pseudomonas savastanoi pv. savastanoi in olive knots. | the endophytic phase of pseudomonas savastanoi pv. savastanoi in olive stems and the structural and ultrastructural histogenesis of olive knots have been studied. construction of a stable plasmid vector expressing the green fluorescent protein, in combination with the use of in vitro olive plants, allowed real-time monitoring of p. savastanoi pv. savastanoi infection. the infection process was also examined by bright field and epifluorescence microscopy as well as by scanning and transmission el ... | 2009 | 21255279 |
| genome sequence analyses of pseudomonas savastanoi pv. glycinea and subtractive hybridization-based comparative genomics with nine pseudomonads. | bacterial blight, caused by pseudomonas savastanoi pv. glycinea (psg), is a common disease of soybean. in an effort to compare a current field isolate with one isolated in the early 1960s, the genomes of two psg strains, race 4 and b076, were sequenced using 454 pyrosequencing. the genomes of both psg strains share more than 4,900 highly conserved genes, indicating very low genetic diversity between psg genomes. though conserved, genome rearrangements and recombination events occur commonly with ... | 2011 | 21304594 |