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the identification of moraxella bovis and neisseria ovis from the eyes of cattle and sheep.the cultural characteristics of moraxella bovis and neisseria ovis from eyes of cattle and sheep were examined to determine which tests precisely identified the isolates. the elongation test to distinguish the bacillary m vovis from the coccal n ovis, the nitrate reduction and the litmus milk tests were found to be the most reliable.1979504803
isolation of neisseria ovis from an aborted bovine foetus. 1978670446
isolation of listeria monocytogenes type 04 from cases of keratoconjunctivitis in cattle and sheep.keratoconjunctivitis in cattle and sheep is usually recognized as a summer problem. the infective agency usually isolated from cattle is moraxella bovis. in sheep neisseria ovis and rickettsia (colesiota) conjunctivae are expected to be found. this report describes isolations of listeria monocytogenes from eye inflammations in housed cattle and sheep.1975806058
comparison of the cell envelope proteins of micrococcus cryophilus with those of neisseria dnd branhamella species.in an attempt to elucidate the relation between micrococcus cryophilus, neisseria caviae, neisseria ovis, and branhamella catarrhalis, fractions derived from outer membranes of a strain of each organism were examined for protein composition by sds - polyacrylamide gel electrophoresis. micrococcus cryophilus outer membrane protein showed extensive similarities to that of n. ovis and contained a heat-modifiable protein which behaved almost identically with the corresponding bands previously shown ...19761260534
isolation of neisseria ovis and a colesiota conjunctivae-like organism from cases of kerato-conjunctivitis in reindeer in northern norway. 19761266683
keratoconjunctivitis associated with neisseria ovis infection in a herd of goats. 19902090273
further data to the aetiology, pathogenesis and therapy of infectious bovine keratoconjunctivitis.out of a total of 224 bovine eye secretions, 126 moraxella bovis and 64 neisseria ovis strains were isolated. the pathogenesis and histological lesions caused by neisseria ovis have been studied on the eyes of three calves naturally affected with ibk, using electron microscopy. neisseria ovis caused in 1-12 weeks old calves acute, transient and mostly benign serous conjunctivitis with only slight affection of the cornea. more rarely erosions and even ulceration of the cornea have been observed. ...19892612133
moraxella ovis in cases of infectious bovine keratoconjunctivitis (ibk) in israel. 19883188722
ovine keratoconjunctivitis experimentally induced by instillation of mycoplasma conjunctivae.five sheep, free from mycoplasma conjunctivae and ocular chlamydia infection, were experimentally inoculated with m. conjunctivae and five more sheep were exposed to the infection by contact. keratoconjunctivitis developed in all ten sheep. as in natural outbreaks of infectious keratoconjunctivitis (ikc), clinical signs were generally moderate and transient, and recurred in some sheep. m. conjunctivae was detected throughout the 53-day observation period. clinical diagnosis was confirmed by hist ...19883218062
the occurrence of mycoplasma conjunctivae in the netherlands and its association with infectious keratoconjunctivitis in sheep and goats.the occurrence of mycoplasma conjunctivae in the netherlands is reported for the first time. m. conjunctivae was detected in all five sheep flocks examined with clinical signs of infectious keratoconjunctivitis (ikc) and in none of five sheep flocks without these clinical signs. these observations suggest a primary aetiological role of this organism in ikc in the netherlands. m. conjunctivae was also detected on a goat farm with clinical signs of ikc. experiences in culturing this organism are r ...19883413973
haemagglutination by neisseria ovis isolated from the eyes of sheep. 19873618012
mycoplasma bovoculi infection increases ocular colonization by moraxella ovis in calves.to determine whether infection with mycoplasma bovoculi increases ocular colonization of cattle eyes with moraxella bovis and other bacteria, colonization of ocular gram-negative bacteria were measured in eyes of cattle infected with mycoplasma bovoculi. strains of moraxella ovis were chosen because these are among the most commonly isolated species of gram-negative bacteria from cattle eyes. five strains of m ovis were characterized biochemically and by pilus structure, permitting the recogniti ...19863729120
exposure of bovine cornea to different strains of moraxella bovis and to other bacterial species in vitro.a collection of strains of moraxella bovis, some pathogenic and some non-pathogenic in cattle, together with other m. bovis preparations, neisseria ovis, staphylococcus aureus and moraxella non-liquefaciens were studied by scanning electron microscopy for their affinity to bovine corneal preparations in vitro. the in vitro procedure provides a convenient method for studies on host-pathogen interactions at the early stage of pathogenesis. the results corresponded well with the pathogenicity of th ...19854031135
mycoplasma and associated bacteria isolated from ovine pink-eye.a mycoplasma was recovered from the untreated conjunctival membranes of nine sheep affected by pink-eye. it was neither isolated from the conjunctiva of treated animals which were affected nor from the conjunctiva of normal animals either in contact or not in contact with affected animals. bacteria found on normal conjunctival membranes were neisseria ovis, escherichia coli, staphylococcus epidermididis, streptococcus and bacillus spp. bacteria found in clinical cases of pink-eye were n. ovis, e ...19714251781
experimental infection of the ovine cornea with neisseria ovis. 19715581449
[bacteriological diagnosis of neisseria ovis. apropos of a strain isolated from keratoconjunctivitis of lambs].the authors report a case of bacteriologic diagnosis of neisseria ovis isolated from a keratoconjunctivitis of lamb. difficulties of diagnosis and taxonomic position of n. ovis are briefly discussed.19826760065
phylogenetic relationships between some members of the genera neisseria, acinetobacter, moraxella, and kingella based on partial 16s ribosomal dna sequence analysis.we obtained 16s ribosomal dna (rdna) sequence data for strains belonging to 11 species of proteobacteria, including the type strains of kingella kingae, neisseria lactamica, neisseria meningitidis, moraxella lacunata subsp. lacunata, [neisseria] ovis, moraxella catarrhalis, moraxella osloensis, [moraxella] phenylpyruvica, and acinetobacter lwoffii, as well as strains of neisseria subflava and acinetobacter calcoaceticus. the data in a distance matrix constructed by comparing the sequences suppor ...19947520730
relatedness of three species of "false neisseriae," neisseria caviae, neisseria cuniculi, and neisseria ovis, by dna-dna hybridizations and fatty acid analysis.dna-dna hybridization was used to determine the levels of genomic relatedness of the three species of "false neisseriae," neisseria caviae, neisseria cuniculi, and neisseria ovis. the reference strains of these species exhibited high levels of intraspecies relatedness (93 to 100% for n. caviae, 79 to 100% for n. cuniculi, and 68 to 100% for n. ovis) but low levels of interspecific relatedness (less than 34%) to each other and to various species belonging to the beta subclass of the proteobacteri ...19938494736
occurrence of autoantibodies to cilia in lambs with a 'coughing syndrome'.a respiratory disease of lambs that has been termed the 'coughing syndrome' has been observed in the mid-western region of the united states of america. mycoplasma ovipneumoniae (m. ovipneumoniae) and mycoplasma arginini (m. arginini) were routinely isolated from the respiratory tract of lambs with this disease. a high level of antibodies reactive with ovine cilia of the upper respiratory tract was detected in the sera from many of the lambs in affected flocks but not in sera of lambs from unaff ...19989730216
association of moraxella ovis with keratoconjunctivitis in mule deer and moose in wyoming.six cases of infectious keratoconjunctivitis (ikc) in mule deer (odocoileus hemionus) and moose (alces alces) in wyoming (usa) were investigated during fall and winter of 1995 and 1996. excessive lacrimation, mucopurulent conjunctivitis, keratitis, and corneal opacity were observed in mule deer. moose had severe mucopurulent conjunctivitis, keratitis, and corneal ulceration. hemolytic, non-piliated moraxella ovis was isolated from two mule deer and two moose. we attempted to reproduce ikc in thr ...200010813605
bacteria and nematodes in the conjunctiva of mule deer from wyoming and utah.swabs of conjunctiva were collected from 44 live and 226 hunter-harvested mule deer (odocoileus hemionus) from wyoming and utah (usa). we identified 29 gram negative and 22 gram positive bacterial taxonomic categories, but many isolates from hunter-harvested animals were environmental contaminants. staphylococcus spp. and micrococcus spp. were the most common gram positive bacteria isolated, and enterobacter spp., escherichia coli, and pseudomonas spp. were common gram negative bacteria isolated ...200011085445
infectious bovine keratoconjunctivitis and lymphofollicular hyperplasia of the third eyelid in heifers.on a dairy cattle farm, infectious bovine keratoconjunctivitis was diagnosed in 29 (24%) calves and heifers aged from 2 weeks to 1 year old. the highest infection rate (18%) occurred in animals aged 3-6 months. the bacteriological examination of swabs from the affected animals yielded several species of bacteria: moraxella bovis, neisseria ovis, n. cuniculi, plasma coagulase-negative staphylococcus spp., alpha-haemolytic streptococcus spp., arcanobacterium pyogenes and escherichia coli. moraxell ...200111315524
a neisseria species associated with infectious keratoconjunctivitis of sheep--neisseria ovis nov. spec. 196014417198
recovery of moraxella ovis from the bovine respiratory tract and differentiation of moraxella species by tdna-intergenic spacer pcr.the purpose of the present study was to identify moraxella (m.)--like organisms recovered from calves suffering from respiratory disease down to species level by means of tdna-intergenic spacer length polymorphism analysis (tdna-pcr), and to perform antimicrobial susceptibility testing of these isolates using an agar dilution technique. a total of 16 isolates originating from 12 unrelated occasions were identified as moraxella ovis, and tdna fingerprinting showed clear delineation from other mor ...200717141983
moraxella bovoculi sp. nov., isolated from calves with infectious bovine keratoconjunctivitis.eighteen isolates of a gram-negative coccus (strain 237(t)) were cultured from the eyes of dairy and beef calves affected with infectious bovine keratoconjunctivitis (ibk; 'pinkeye') in northern california, usa, during summer 2002. these isolates had near full-length (1397 bp) 16s rrna gene sequences that clustered into three groups with 99.9 % sequence similarity. on the basis of 16s rrna gene sequence, the isolates were most closely associated with moraxella bovis and moraxella ovis in clade i ...200717392208
identification and characterization of complete rtx operons in moraxella bovoculi and moraxella ovis.to determine if moraxella bovoculi (m. bovoculi), a recently characterized coccoid moraxella that was isolated from the eyes of calves affected with infectious bovine keratoconjunctivitis (ibk), and moraxella ovis (m. ovis), originally isolated from sheep with conjunctivitis, possessed genes encoding rtx proteins, genomic dna was amplified with oligonucleotide primers targeting rtx operon genes of moraxella bovis (m. bovis). complete classical rtx operons composed of rtxcabd genes closely linked ...200717561358
differentiation of moraxella bovoculi sp. nov. from other coccoid moraxellae by the use of polymerase chain reaction and restriction endonuclease analysis of amplified dna.moraxella ovis was historically the only coccoid moraxella identified in cultures of ocular fluid from cattle with infectious bovine keratoconjunctivitis (ibk) and could be morphologically and biochemically differentiated from moraxella bovis. moraxella bovoculi sp. nov. is a recently characterized moraxella isolated from ulcerated eyes of calves with ibk in northern california in 2002. like moraxella ovis, m. bovoculi sp. nov. is a gram-negative coccus/diplococcus. all 18 original isolates of m ...200717823397
development and evaluation of a multiplex real-time pcr assay for the detection and differentiation of moraxella bovis, moraxella bovoculi and moraxella ovis in pure culture isolates and lacrimal swabs collected from conventionally raised cattle.aim:ôçé to develop a multiplex real-time pcr assay for the detection and differentiation of moraxella bovis (m.-ábovis), m.-ábovoculi and m.-áovis. methods and results:ôçé the multiplex real-time pcr assay was validated on three reference strains, 57 pure culture isolates and 45 lacrimal swab samples. all reference strains were identified correctly with no cross-reactions between species. sequencing of 53 of the 57 culture isolates confirmed the results obtained with the multiplex real-time pcr, ...201121827584
descriptive epidemiology of moraxella bovis, moraxella bovoculi and moraxella ovis in beef calves with naturally occurring infectious bovine keratoconjunctivitis (pinkeye).problem addressed: infectious bovine keratoconjunctivitis (ibk) is an ocular disease that causes substantial weight loss in beef calves. objective: the objective of this study was to evaluate the association between moraxella bovoculi, moraxella bovis and moraxella ovis and ibk incidence. methods and approach: a cohort design was used. from 239 calves and 478 eyes, 77 randomly chosen eyes were monitored for m. bovoculi, m. bovis, m. ovis and ibk incidence over 4 months. one hypothesis tested was ...201121962410
phylogenetic analysis and genetic diversity of 3' region of rtxa gene from geographically diverse strains of moraxella bovis, moraxella bovoculi and moraxella ovis.the cytotoxin a (mbxa) is one of the main virulence factors of moraxella bovis involved in the pathogenesis of infectious bovine keratoconjunctivitis (ibk). moraxella ovis and moraxella bovoculi, suspected to be associated with infectious keratitis in sheep and cattle respectively, also have a gene that encodes the cytotoxin a (mova and mbva, respectively). the aim of this study was to determine the molecular sequence of the 3' region of the cytotoxin gene of moraxella spp. strains isolated from ...201526036790
moraxella spp. isolated from field outbreaks of infectious bovine keratoconjunctivitis: a retrospective study of case submissions from 2010 to 2013.infectious bovine keratoconjunctivitis (ibk), also known as pinkeye, is the most costly eye disease of cattle. the principal etiologic agent of ibk is the gram-negative bacterium moraxella bovis. however, there have been reports of ibk outbreaks associated with moraxella bovoculi. a retrospective study of ibk diagnostic cases submitted from july 1, 2010 through october 31, 2013 was conducted. included in the study were 1,042 moraxella isolates from 1,538 swabs of lacrimal secretions collected fr ...201425261461
normal bacterial conjunctival flora in the huacaya alpaca (vicugna pacos).to describe the bacterial flora of the normal conjunctiva of huacaya alpacas (vicugna pacos) and to determine the effect of age and gender on this flora.201625581469
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