| [occurrence of species from acinetobacter genus in clinical material and other sources]. | the analysis regarded 304 strains of acinetobacter genus isolated from various diagnostic materials, objects from hospital environment and from non-hospital sources (soil, water, various animals). applying api zone system, five species were isolated: acinetobacter juni, (18.42%), acinetobacter baumanii (70.39%), acinetobacter haemolyticus (5.59%), acinetobacter lwoffii (4.6%) and acinetobacter johnsonii (0.99%). most frequently isolated species were present in purulent materials and in samples f ... | 1992 | 1297033 |
| [sensitivity of bacteria of the genus acinetobacter to antibiotics and ofloxacin]. | between 1989-1989 276 strains of acinetobacter genus were isolated which contained: acinetobacter calcoaceticus subsp. anitratus (n = 167), acinetobacter calcoaceticus subsp. iwoffi (n- = 83), acinetobacter haemolyticus (n = 26). their sensitivity to aminoglycoside antibiotics, beta-lactams, tetracyclines, chloramphenicol, colistin, and ofloxacin was tested. more than 90% of strains were sensitive to colistin and ofloxacin. the sensitivity to remaining antibiotics differentiated depending on spe ... | 1991 | 1823372 |
| a study of the relationships between antibiotic resistance phenotypes, phage-typing and biotyping of 117 clinical isolates of acinetobacter spp. | two typing systems were used to conduct an epidemiological study of acinetobacter and to establish their relationship to antibiotic resistance phenotypes. biotyping was performed with biochemical tests according to the new definition of acinetobacter baumannii (18 biotypes). phage typing included two complementary systems: 125 phage-types and 25 subtypes. resistance phenotype analysis included 11 antibiotics. the results of the study showed that: (1) nine phage-types or subtypes (67%) and two gr ... | 1990 | 1974906 |
| lack of pqq dependent glucose dehydrogenase apoenzyme in some acinetobacter spp. | seventy four asaccharolytic strains of acinotobacter were tested for the presence of pqq dependent glucose dehydrogenase apoenzyme. this apoenzyme could not be detected in any of five strains of acinetobacter haemolyticus; it was also lacking in one strain of each a. junii and a. lwoffi. | 1990 | 2176072 |
| [serology of achromobacter haemolyticus]. | | 1967 | 4925332 |
| nucleic acid homologies among oxidase-negative moraxella species. | the deoxyribonucleic acid (dna) base composition and dna homologies of more than 40 strains of oxidase-negative moraxella species were determined. these bacteria have also been identified as belonging to the mima-herellea-acinetobacter group and the bacterium anitratum group, as well as to several other genera including achromobacter and alcaligenes. the dna base content of these strains ranged from 40 to 46% guanine plus cytosine. dna-dna competition experiments distinguished five groups whose ... | 1970 | 5413826 |
| in vitro activity of beta-lactamase inhibitors against clinical isolates of acinetobacter species. | acinetobacter is an important cause of nosocomial infections, and it is often resistant to many antibiotics. in a search for alternative agents, three beta-lactamase inhibitors (sulbactam, clavulanate, and tazobactam) and five beta-lactam antibiotics (imipenem, ceftazidime, ceftriaxone, cefotaxime, and piperacillin) were tested against 68 unique clinical isolates of acinetobacter species. minimum inhibitory concentrations were determined by a broth microdilution method. using temperature sensiti ... | 1995 | 7628190 |
| identification of acinetobacter genomic species by amplified ribosomal dna restriction analysis. | a total of 53 field and reference strains, including the type strains of the seven named species (nomenspecies) and belonging to the 18 described genomic species (dna groups) of the genus acinetobacter, were studied by amplified ribosomal dna restriction analysis (ardra). restriction analysis with the enzymes alui, cfoi, mboi, rsai, and mspi of the enzymatically amplified 16s rrna genes allowed us to identify all species except the genomic species 4 (acinetobacter haemolyticus) and 7 (a. johnson ... | 1995 | 7699025 |
| characterization of the aac(6')-ik gene of acinetobacter sp. 6. | the aac(6')-ik gene which confers resistance to aminoglycosides in acinetobacter sp. 6 cip a165 was characterized. the resistance gene was identified as a coding sequence of 438 bases pairs corresponding to a protein with a calculated mass of 16627 da. alignment of aac(6')-ik with aac(6')-ig from acinetobacter haemolyticus indicated 83% identity. like aac(6')-ig of a. haemolyticus and aac(6')-ij of acinetobacter sp. 13, the aac(6')-ik gene was apparently located in the chromosome and was species ... | 1994 | 8001769 |
| structure of acinetoferrin, a new citrate-based dihydroxamate siderophore from acinetobacter haemolyticus. | from low-iron cultures of acinetobacter haemolyticus atcc 17906, a new hydroxamate siderophore was purified by xad-7 adsorption followed by preparative thin layer chromatography. the siderophore, named acinetoferrin, released citric acid, 1,3-diaminopropane and (e)-2-octenoic acid upon hydrolysis with hcl, reductive hydrolysis with hi and oxidation with periodate, respectively. structure elucidation by a combination of nmr spectroscopy and positive fast atom bombardment mass spectrometry reveale ... | 1994 | 8148619 |
| characterization of acinetobacter haemolyticus aac(6')-ig gene encoding an aminoglycoside 6'-n-acetyltransferase which modifies amikacin. | the amikacin resistance gene acc(6')-ig of acinetobacter haemolyticus bm2685 encoding an aminoglycoside 6'-n-acetyltransferase was characterized. the gene was identified as a coding sequence of 438 bp corresponding to a protein with a calculated mass of 16,522 da. analysis of the deduced amino acid sequence suggested that it was the fourth member of a subfamily of aminoglycoside 6'-n-acetyltransferases. the resistance gene was not transferable either by conjugation to escherichia coli or to acin ... | 1993 | 8257129 |
| infective endocarditis of an interventricular patch caused by acinetobacter haemolyticus. | a case of infective endocarditis caused by acinetobacter haemolyticus affecting and interventricular patch is reported. the patient, a 21-year-old man with a fallot's tetralogy who had undergone cardiovascular surgery several years before, received imipenem and gentamicin for 6 and 4 weeks respectively and showed a good response without needing surgical treatment. endocarditis by acinetobacter species is very unusual and, to our knowledge, this is the first reported case of infective endocarditi ... | 1995 | 8522385 |
| evaluation of the ability of a commercial system to identify acinetobacter genomic species. | a collection of 130 acinetobacter strains identified by dna hybridization to 18 different genomic species was used to assess the ability of the api 20ne system (biomérieux, france) to identify acinetobacter genomic species and to determine its accuracy. fifty-eight (87%) of the 67 strains of genomic species defined in the database (version 5.1) were identified to the appropriate genomic species. the acinetobacter baumannii strains and the acinetobacter haemolyticus strains were all identified co ... | 1996 | 8781881 |
| activities of beta-lactams against acinetobacter genospecies as determined by agar dilution and e-test mic methods. | the agar dilution mic method was used to test activities of ticarcillin, ticarcillin-clavulanate, amoxicillin, amoxicillin-clavulanate, ampicillin, ampicillin-sulbactam, piperacillin, piperacillin-tazobactam, inhibitors alone, ceftazidime, and imipenem against 237 acinetobacter genospecies. a total of 93.2% of strains were beta-lactamase positive by the chromogenic cephalosporin method. overall, ampicillin-sulbactam was the most active combination against all strains (mic at which 50% of the iso ... | 1997 | 9087486 |
| structural and serological characterisation of the o-antigenic polysaccharide of the lipopolysaccharide from acinetobacter haemolyticus strain atcc 17906. | a polysaccharide containing 2-acetamido-2-deoxy-d-galacturonic acid (galnaca), 2.4-diacetamido-2,4,6-trideoxy-d-glucose (quinac4nac), and d-alanine (ala) was isolated from the water-soluble lipopolysaccharide (lps) originating from the reference strain for acinetobacter haemolyticus (dna group 4) strain atcc 17906. the polysaccharide, characterised by means of monosaccharide analyses and nmr studies, was shown to be based on a linear trisaccharide repeating unit, as shown below, with the alanine ... | 1997 | 9108245 |
| phylogenetic relationship of the twenty-one dna groups of the genus acinetobacter as revealed by 16s ribosomal dna sequence analysis. | the inter- and intrageneric relationships of members of the genus acinetobacter were investigated by performing a comparative sequence analysis of pcr-amplified 16s ribosomal dnas (rdnas) from 21 strains representing all of the dna groups that have been described. phylogenetic treeing confirmed that acinetobacter spp. form a coherent cluster within the gamma subdivision of the class proteobacteria that includes strains with overall levels of 16s rdna sequence similarity of more than 94%. the ana ... | 1997 | 9226915 |
| structural investigation of the lipopolysaccharide from acinetobacter haemolyticus strain nctc 10305 (atcc 17906, dna group 4). | the structure of the lipopolysaccharide (lps) from acinetobacter haemolyticus strain nctc 10305 (dna group 4) was elucidated by means of analytical chemistry, nmr spectroscopy and fast-atom-bombardment mass spectrometry. several oligosaccharides were obtained after deacylation or successive de-o-acylation, dephosphorylation, reduction, and de-n-acylation of lps. in the major fraction of the lps, the core is attached to the lipid a through d-glycero-d-talo-2-octulopyranosonic acid (ko), whereas i ... | 1997 | 9249012 |
| loss of intrinsic aminoglycoside resistance in acinetobacter haemolyticus as a result of three distinct types of alterations in the aac(6')-ig gene, including insertion of is17. | the distribution of the aac(6')-ig gene, encoding aminoglycoside 6'-n-acetyltransferase-ig [aac(6')-ig], was studied in 96 acinetobacter haemolyticus strains and 12 proteolytic acinetobacter strains, including acinetobacter genomospecies 6, 13, and 14 and 3 unnamed species assigned to this genomic group by dna-dna hybridization. this gene was detected by dna-dna hybridization in all 96 a. haemolyticus strains and by pcr in 95 strains but was not detected in strains of other species, indicating t ... | 1997 | 9420034 |
| cloning and characterization of 3-deoxy-d-manno-oct-2-ulosonic acid (kdo) transferase genes (kdta) from acinetobacter baumannii and acinetobacter haemolyticus. | 3-deoxy-d-manno-oct-2-ulosonic acid (kdo) transferases (kdta) are multifunctional glycosyltransferases with primary structures of low similarity. totally degenerated primers were deduced from two stretches of identical amino acids between known kdta sequences and used to amplify by pcr a kdta-specific fragment from acinetobacter baumannii atcc 15308 dna which was then applied as a probe for the cloning and sequencing of the complete kdo transferase gene. with conserved pcr primers for this struc ... | 1998 | 9660198 |
| chemical and antigenic structure of the o-polysaccharide of the lipopolysaccharides from two acinetobacter haemolyticus strains differing only in the anomeric configuration of one glycosyl residue in their o-antigens. | in a previous study [pantophlet, r., brade, l., dijkshoorn, l., and brade, h. (1998) j. clin. microbiol. 36, 1245-1250] the o-polysaccharide of the lipopolysaccharides (lps) from acinetobacter haemolyticus strains 57 and 61 exhibited indistinguishable banding-patterns following western blot and immunostaining with homologous or heterologous rabbit antiserum. in this report, the molecular basis for the observed cross-reactivity was elucidated, by determining the chemical structure of the polysacc ... | 1999 | 10406970 |
| synthesis of neoglycoproteins containing d-glycero-d-talo-oct-2-ulopyranosylonic acid (ko) ligands corresponding to core units from burkholderia and acinetobacter lipopolysaccharide. | glycal esters of kdo derivatives were converted into 2,3-anhydro intermediates, which were transformed into d-glycero-d-talo-oct-2-ulopyranosylonic acid (ko), as well as 3-o- and 4-o-p-nitrobenzoyl-ko derivatives. the exo-allyl orthoester derivative, methyl [5,7,8-tri-o-acetyl-4-o-(4-nitrobenzoyl)-2,3-o-[(1-exo-allyloxy)-ethylidene]-d-glycero-beta-d-talo-oct-2-ulopyranos]onate, prepared from the 4-o-pnbz-protected ko derivative, was elaborated into the alpha-ko allyl ketoside, the reducing disac ... | 2000 | 11128584 |
| monoclonal antibodies against 3-deoxy-alpha-d-manno-oct-2-ulosonic acid (kdo) and d-glycero-alpha-d-talo-oct-2-ulosonic acid (ko). | monoclonal antibodies (mabs) were obtained after immunization of mice with neoglycoconjugates containing as a ligand the disaccharide kdo(2-->4)ko or ko(2-->4)kdo, representing structural elements of the core region of lipopolysaccharides (lpss) from acinetobacter haemolyticus and burkholderia cepacia, respectively. one antibody, s67-9, bound with high specificity to ko(2-->4)kdo-bsa showing no cross reactivity with kdo(2-->4)kdo-bsa and the other antigens tested. a second mab, s68-12, bound pre ... | 2002 | 12537694 |
| synergistic relationships in algal-bacterial microcosms for the treatment of aromatic pollutants. | the potential of algal-bacterial microcosms was studied for the biodegradation of salicylate, phenol and phenanthrene. the isolation and characterization of aerobic bacterial strains capable of mineralizing each pollutant were first conducted. ralstonia basilensis was isolated for salicylate degradation, acinetobacter haemolyticus for phenol and pseudomonas migulae and sphingomonas yanoikuyae for phenanthrene. the green alga chlorella sorokiniana was then cultivated in the presence of the pollut ... | 2003 | 12688473 |
| [characteristics of strains of acinetobacter spp. after culture in various media]. | growth, surface properties, production of lipase, histamine and some signal molecules (n-acyl-homoserine lactones, ahls) in four acinetobacter baumannii strains, in one acinetobacter lwoffii and in one acinetobacter haemolyticus strain cultivated in three complex media were studied. more intensive growth of bacterial suspensions was observed in mueller-hinton broth and in brain heart infusion in comparison with growth in proteose peptone. greater growth of bacterial strains (with the exception o ... | 2003 | 12822537 |
| membrane dynamics of the amphiphilic siderophore, acinetoferrin. | acinetobacter haemolyticus is an antibiotic resistant, pathogenic bacterium responsible for an increasing number of hospital infections. acinetoferrin (af), the amphiphilic siderophore isolated from this organism, contains two unusual trans-2-octenoyl hydrocarbon chains reminiscent of a phospholipid structural motif. here, we have investigated the membrane affinity of af and its iron complex, fe-af, using small and large unilamellar phospholipid vesicles (suv and luv) as model membranes. af show ... | 2005 | 15701007 |
| increasing ciprofloxacin resistance among prevalent urinary tract bacterial isolates in the gaza strip. | this study aims to assess common organisms causing urinary tract infection (uti) in the gaza strip, palestinian authority and to examine the incidence of ciprofloxacin resistance in the strains of bacteria isolated from patients suspected with uti over a six-month period. | 2005 | 16123829 |
| increasing ciprofloxacin resistance among prevalent urinary tract bacterial isolates in gaza strip, palestine. | this article presents the incidence of ciprofloxacin resistance among 480 clinical isolates obtained from patients with urinary tract infection (uti) during january to june 2004 in gaza strip, palestine. the resistance rates observed were 15.0% to ciprofloxacin, 82.5% to amoxycillin, 64.4% to cotrimoxazole, 63.1% to doxycycline, 32.5% to cephalexin, 31.9% to nalidixic acid, and 10.0% to amikacin. high resistance to ciprofloxacin was detected among acinetobacter haemolyticus (28.6%), staphylococc ... | 2005 | 16192681 |
| evaluation of pyrrolidonyl arylamidase for the identification of nonfermenting gram-negative rods. | to evaluate the activity of pyrrolidonyl arylamidase (pyr) for the differentiation and identification of nonfermenting gram negative rods (nfgnr), 293 isolates were tested. a 24 h culture of each test organism was prepared. from this a 108-109 cfu/ml suspension was added to 0.25 ml of sterile physiologic solution. a pyr disk was then added and the test was incubated for 30 minutes at 35-37 degrees c, at environmental atmosphere. reading was done by adding 1 drop of cinnamaldehyde reagent. strain ... | 2007 | 16822636 |
| bla(oxa-51)-type beta-lactamase genes are ubiquitous and vary within a strain in acinetobacter baumannii. | we investigated the spread of bla(oxa-51)-type beta-lactamase genes in 200 acinetobacter spp. clinical strains isolated in argentina from 1982 to 2005. bla(oxa-51)-type genes were present in all acinetobacter baumannii isolates tested (n=194), whereas they were not detected in two acinetobacter haemolyticus, two genomic species 10 or two acinetobacter lwoffii isolates. the bla(oxa-51)-type alleles varied within a strain and were found in six different a. baumannii pulsed-field gel electrophoresi ... | 2006 | 16844350 |
| shiga toxin 2-producing acinetobacter haemolyticus associated with a case of bloody diarrhea. | we report the first shiga toxin 2-producing acinetobacter haemolyticus strain that was isolated from the feces of a 3-month-old infant with bloody diarrhea. usual enteropathogenic bacteria were not detected. this finding suggests that any shiga toxin-producing microorganism capable of colonizing the human gut may have the potential to cause illness. | 2006 | 17021124 |
| hexavalent chromium reduction by acinetobacter haemolyticus isolated from heavy-metal contaminated wastewater. | possible application of a locally isolated environmental isolate, acinetobacter haemolyticus to remediate cr(vi) contamination in water system was demonstrated. cr(vi) reduction by a. haemolyticus seems to favour the lower concentrations (10-30 mg/l). however, incomplete cr(vi) reduction occurred at 70-100 mg/l cr(vi). initial specific reduction rate increased with cr(vi) concentrations. cr(vi) reduction was not affected by 1 or 10 mm sodium azide (metabolic inhibitor), 10 mm of po(4)3-, so4(2-) ... | 2007 | 17188812 |
| biological detoxification of cr(vi) using wood-husk immobilized acinetobacter haemolyticus. | acinetobacter haemolyticus, a gram-negative aerobic locally isolated bacterium, immobilized on wood-husk showed the ability to detoxify cr(vi) to cr(iii). wood-husk, a natural cellulose-based support material, packed in an upward-flow column was used as support material for bacterial attachment. around 97% of the cr(vi) in wastewater containing 15 mg l(-1) of cr(vi) was reduced at a flow rate of 8.0 ml min(-1). the wastewater containing cr(vi) was added with liquid pineapple wastewater as nutrie ... | 2007 | 17368716 |
| isolation and characterization of a novel n-alkane-degrading strain, acinetobacter haemolyticus ar-46. | strain ar-46, isolated and identified as acinetobacter haemolyticus, evolutionally distant from the known hydrocarbon-degrading acinetobacter spp., proved to have excellent long-chain n-alkane-degrading ability. this is the first detailed report on an n-alkane-utilizing strain belonging to this species. the preferred substrate is n-hexadecane, with an optimal temperature of 37 degrees c under aerobic conditions. five complete and two partial open reading frames were sequenced and correlated with ... | 2007 | 17542497 |
| molecular epidemiology of imipenem-resistant acinetobacter haemolyticus and acinetobacter baumannii isolates carrying plasmid-mediated oxa-40 from a portuguese hospital. | | 2007 | 17606684 |
| molecular characterisation of the metallo-beta-lactamase genes in imipenem-resistant gram-negative bacteria from a university hospital in southern taiwan. | in this study, 260 non-replicate imipenem-resistant gram-negative bacteria isolated between january 2002 and december 2006 were subjected to a screening test for detection of metallo-beta-lactamase (mbl) using the etest containing imipenem and ethylene diamine tetra-acetic acid (edta). among the 260 strains, 123 (47.3%) appeared to produce mbl. of these 123 strains, 113 (91.9%) were found by polymerase chain reaction (pcr) to carry mbl genes of types blavim-2, blavim-3, blavim-11 (blavim-11a), b ... | 2008 | 18804966 |
| tuberculosis in southern chinese renal-transplant recipients. | to analyze the characteristics of tuberculosis (tb) in southern chinese renal transplant recipients, and summarize the corresponding experiences in diagnosis and management. | 2008 | 19040560 |
| pilot-scale removal of chromium from industrial wastewater using the chromebac system. | the enzymatic reduction of cr(vi) to cr(iii) by cr(vi) resistant bacteria followed by chemical precipitation constitutes the chromebac system. acinetobacter haemolyticus was immobilized onto carrier material inside a 0.2m(3) bioreactor. neutralized electroplating wastewater with cr(vi) concentration of 17-81 mg l(-1) was fed into the bioreactor (0.11-0.33 m(3)h(-1)). complete cr(vi) reduction to cr(iii) was obtained immediately after the start of bioreactor operation. together with the flocculat ... | 2010 | 20185301 |
| biodegradability of bacterial surfactants. | this work aimed at evaluating the biodegradability of different bacterial surfactants in liquid medium and in soil microcosms. the biodegradability of biosurfactants by pure and mixed bacterial cultures was evaluated through co(2) evolution. three bacterial strains, acinetobacter baumanni lbbma es11, acinetobacter haemolyticus lbbma 53 and pseudomonas sp. lbbma 101b, used the biosurfactants produced by bacillus sp. lbbma 111a (mixed lipopeptide), bacillus subtilis lbbma 155 (lipopeptide), flavob ... | 2010 | 21053055 |
| dioxygenases in burkholderia ambifaria and yersinia pestis that hydroxylate the outer kdo unit of lipopolysaccharide. | several gram-negative pathogens, including yersinia pestis, burkholderia cepacia, and acinetobacter haemolyticus, synthesize an isosteric analog of 3-deoxy-d-manno-oct-2-ulosonic acid (kdo), known as d-glycero-d-talo-oct-2-ulosonic acid (ko), in which the axial hydrogen atom at the kdo 3-position is replaced with oh. here we report a unique kdo 3-hydroxylase (kdoo) from burkholderia ambifaria and yersinia pestis, encoded by the bamb_0774 (bakdoo) and the y1812 (ypkdoo) genes, respectively. when ... | 2010 | 21178073 |
| optimization of medium for lipase production by acinetobacter haemolyticus from healthy human skin. | a lipase producing acinetobacter haemolyticus ta106 was isolated from healthy human skin of tribal population. the maximum activity of 55 u/ml was observed after medium optimization using the "one variable at a time" and the statistical approaches. the optimal composition of the medium was determined as (% w/v or v/v): tryptone--1, yeast extract--0.5, sodium chloride-1, olive oil-1, tween-80 1, manganese sulphate--5 mm, sucrose--1, ph-7. it was found that maximum production occurred in late log ... | 2010 | 21506503 |
| Genetic Diversity of Endophytic Diazotrophs of the Wild Rice, Oryza alta and Identification of the New Diazotroph, Acinetobacter oryzae sp. nov. | Thirty-three endophytic diazotrophs were isolated from surface-sterilized leaves, stem, and roots of wild rice Oryza alta. The SDS-PAGE profile of total protein and insertion sequence-based polymerase chain reaction (IS-PCR) fingerprinting grouped the isolates into four clusters (I-IV). The 16S rRNA gene sequence homology of the representative strains B21, B31, B1, and B23 of clusters I, II, III, and IV were assigned to Pseudomonas oleovorans (99.2% similarity), Burkholderia fungorum (99.4% simi ... | 2011 | 22105517 |
| identification of the naturally occurring genes encoding carbapenem-hydrolysing oxacillinases from acinetobacter haemolyticus, acinetobacter johnsonii, and acinetobacter calcoaceticus. | clin microbiol infect abstract: carbapenem resistance is increasingly being reported among acinetobacter species, and results mostly from the expression of acquired carbapenem-hydrolysing oxacillinases (chdls). several acinetobacter species intrinsically possess chromosomal chdl genes: acinetobacter baumannii (bla(oxa-51) ), acinetobacter radioresistens (bla(oxa-23) ), and acinetobacter lwoffii (bla(oxa-134) ). we aimed to identify the progenitors of novel chdl-encoding genes for identification ... | 2011 | 22128805 |
| analysis of the bacterial diversity existing on animal hide and wool: development of a preliminary pcr-restriction fragment length polymorphism fingerprint database for identifying isolates. | twenty-one bacterial strains were isolated from imported cattle hide and rabbit wool using two types of media, nutrient broth, and nutrient broth with serum. the bacteria identified were brevibacillus laterosporus, leclercia adecarboxylata, peptococcus niger, bacillus circulans, raoultella ornithinolytica, bacillus subtilis, bacillus cereus, bacillus thermobacillus, bacillus choshinensis, bacillus sphaericus, acinetobacter haemolyticus, sphingomonas paucimobilis, bacillus thuringiensis, staphylo ... | 2012 | 23451394 |
| genome sequencing and annotation of acinetobacter haemolyticus strain mtcc 9819(t). | the genus acinetobacter consists of 31 validly published species ubiquitously distributed in nature and primarily associated with nosocomial infection. we report the 3.4 mb genome of acinetobacter haemolyticus strain mtcc 9819(t). the genome has a g + c content of 40.0% and includes 3 rrna genes (5s, 23s, 16s) and 65 aminoacyl-trna synthetase genes. | 2013 | 26484055 |
| characterization of plasmids in extensively drug-resistant acinetobacter strains isolated in india and pakistan. | the blandm-1 gene is associated with extensive drug resistance in gram-negative bacteria. this probably spread to enterobacteriaceae from acinetobacter spp., and we characterized plasmids associated with blandm-1 in acinetobacter spp. to gain insight into their role in this dissemination. four clinical ndm-1-producing acinetobacter species strains from india and pakistan were investigated. a plasmid harboring blandm-1, pndm-40-1, was characterized by whole-genome sequencing of acinetobacter bere ... | 2015 | 25421466 |
| multidrug resistance acinetobacter species at the intensive care unit, aseer central hospital, saudi arabia: a one year analysis. | to identify and to determine the antimicrobial susceptibility of acinetobacter baumannii (a. baumannii) clinical isolates from icu at aseer central hospital. | 2016 | 27633307 |
| development and evaluation of species-specific pcr for detection of nine acinetobacter species. | molecular methods have the potential to improve the speed and accuracy of acinetobacter species identification in clinical settings. the goal of this study is to develop species-specific pcr assays based on differences in the rna polymerase beta-subunit gene (rpob) to detect nine commonly isolated acinetobacter species including acinetobacter baumannii, acinetobacter calcoaceticus, acinetobacter pittii, acinetobacter nosocomialis, acinetobacter lwoffii, acinetobacter ursingii, acinetobacter bere ... | 2016 | 27312551 |
| identification, genotypic relation, and clinical features of colistin-resistant isolates of acinetobacter genomic species 13bj/14tu from bloodstreams of patients in a university hospital. | colistin resistance remains rare among clinical isolates of acinetobacter species. we noted the emergence of colistin-resistant bloodstream isolates of the acinetobacter genomic species (gs) 13bj/14tu from patients at a university hospital between 2003 and 2011. we report here, for the first time, the microbiological and molecular characteristics of these isolates, with clinical features of acinetobacter gs 13bj/14tu bacteremia. all 11 available patient isolates were correctly identified as acin ... | 2014 | 24403305 |
| multiplex pcr to detect the genes encoding naturally occurring oxacillinases in acinetobacter spp. | bacteria of the genus acinetobacter are increasingly being isolated in hospitals and are recognized as emerging nosocomial pathogens. species identification is difficult and there is a need for simple molecular methods to differentiate between the species. naturally occurring oxacillinase genes (blaoxa) have been identified in several acinetobacter species and their detection by pcr can aid in species identification. the aim of this study was to develop a multiplex pcr to identify intrinsic blao ... | 2014 | 24288029 |
| surface water isolates of hemolytic and non-hemolytic acinetobacter with multiple drug and heavy metal resistance ability. | acinetobacter in surface waters are a major concern because of their rapid development of resistance to a wide range of antimicrobials and their ability to persist in these waters for a very long time. four surface water isolates of acinetobacter having both multidrug- and multimetal-resistant ability were isolated and identified through biochemical tests and 16s rdna sequencing. based on these analyses, two hemolytic isolates were affiliated with acinetobacter haemolyticus with an accession num ... | 2014 | 24642427 |
| microbiological characteristics and clinical outcomes of acinetobacter spp. endophthalmitis with the first reported case of acinetobacter haemolyticus endophthalmitis. | to present the microbiological details and clinical outcomes of acinetobacter spp. endophthalmitis with the first reported case of a. haemolyticus. | 2017 | 28328294 |
| characterization and toxicity of a phosphate-binding exobiopolymer produced by acinetobacter haemolyticus mg606. | a novel, phosphate-binding exobiopolymer (ebp) produced by acinetobacter haemolyticus mg606 was characterized and its biocompatibility evaluated in raw 264.7 cells and in mice. ebp was identified as a 50 kda heteropolysaccharide composed of pentose and hexose sugars. ebp exhibited cytotoxicity, stimulation of free radical production and loss of mitochondrial and lysosomal integrity in raw 264.7 cells at 500 μg/ml concentration while lower concentrations exhibited no significant (p > 0.05) effect ... | 2017 | 28151444 |
| antimicrobial efficacy and safety of mucoadhesive exopolymer produced by acinetobacter haemolyticus. | this study evaluated five extracellular polymers of bacterial origin possessing mucoadhesive properties for their antimicrobial properties and toxicological characteristics. of the five tested mucoadhesive biopolymers, the extracellular polymer produced by a strain of acinetobacter haemolyticus exhibited broad antimicrobial efficacy towards yersinia enterocolitica, salmonella typhimurium, listeria monocytogenes, escherichia coli o157:h7 and bacillus subtilis. significant (p<0.05) inhibition of g ... | 2017 | 27720965 |
| [bacterial community characterization of rearing water of marine recirculating aquaculture systems for yellow grouper (epinephelus awoara)]. | this study was aimed to reveal the bacterial community characteristics of rearing water of marine recirculating aquaculture system for yellow grouper (epinephelus awoara), and compare the differences between bacterial community structure of healthy rearing water and sick rearing water in order to elucidate the relationship between bacterial community and fish disease. | 2016 | 27373073 |
| synthesis of 5-o-oligoglucosyl extended α-(2→4)-kdo disaccharides corresponding to inner core fragments of moraxellaceae lipopolysaccharides. | the heptose-deficient inner core of the lipopolysaccharide of several pathogenic strains of the moraxellaceae family (moraxella, acinetobacter) and of bartonella henselae, respectively, comprises an α-d-glucopyranose attached to position 5 of kdo. in continuation of the synthesis of fragments of acinetobacter haemolyticus lps, the branched α-glcp-(1 → 5)[α-kdo-(2 → 4)]-α-kdo trisaccharide motif was elaborated. the glycosylation of a suitably protected, α-(2 → 4)-interlinked kdo-disaccharide was ... | 2016 | 26795079 |
| new insights on the palate, lung, and nasal epithelium clone (plunc) proteins: based on molecular and functional analysis of its homolog of yh1/splunc1. | the palate, lung, and nasal epithelium clone (plunc) proteins are intricate immune molecules and arisen questions from them are still unresolved. in order to identify the role of plunc family proteins, we had analyzed its homolog protein yh1/splunc1, which highly expresses in nontumor nasopharyngeal epithelium while expresses weakly in nasopharyngeal carcinoma (npc) tissues. it is found that yh1/splunc1 protein expression level was higher in chronic normal nasopharynx inflammatory cells compared ... | 2016 | 26654795 |
| characterization and upregulation of bifunctional phosphoglucomutase/phosphomannomutase enzyme in an exobiopolymer overproducing strain of acinetobacter haemolyticus. | several members of the acinetobacter spp. produce exobiopolymer (ebp) of considerable biotechnological interest. in a previous study, we reported phosphate removal capacity of ebp produced by acinetobacter haemolyticus. insertional mutagenesis was attempted to develop ebp-overproducing strains of a. haemolyticus and mutant mg606 was isolated. in order to understand the underlying mechanism of overproduction, the ebp overproducing mutant mg606 was analyzed and compared with the wild type counterp ... | 2015 | 26640047 |
| acinetobacter haemolyticus mg606 produces a novel, phosphate binding exobiopolymer. | the present study evaluated an extracellular, novel biopolymer produced by acinetobacter haemolyticus mg606 for its physicochemical properties and phosphate binding mechanism. the exobiopolymer (ebp) was characterized to be majorly polysaccharide in nature consisting of 48.9 kda heteropolysaccharide composed of galactose, glucose, xylose, lyxose, allose, ribose, arabinose, mannose and fructose. maximum phosphate binding efficiency of 25mg phosphate/g of ebp was described by langmuir isotherm and ... | 2015 | 26256326 |
| differentiation of acinetobacter genomic species 13bj/14tu from acinetobacter haemolyticus by use of matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ms). | | 2015 | 26224844 |
| time dependent enhanced resistance against antibiotics & metal salts by planktonic & biofilm form of acinetobacter haemolyticus mmc 8 clinical isolate. | available literature shows paucity of reports describing antibiotic and metal resistance profile of biofilm forming clinical isolates of acinetobacter haemolyticus. the present study was undertaken to evaluate the antibiotic and metal resistance profile of indian clinical isolate of a. haemolyticus mmc 8 isolated from human pus sample in planktonic and biofilm form. | 2014 | 25579150 |
| acinetobacter gandensis sp. nov. isolated from horse and cattle. | we previously reported the presence of an oxa-23 carbapenemase in an undescribed species of the genus acinetobacter isolated from horse dung at the faculty of veterinary medicine, ghent university, belgium. here we include six strains to corroborate the delineation of this taxon by phenotypic characterization, dna-dna hybridization, 16s rrna gene and rpob sequence analysis, % g+c determination, maldi-tof ms and fatty acid analysis. the nearly complete 16s rrna gene sequence of strain ug 60467(t) ... | 2014 | 25225259 |
| microbial ecology of thailand tsunami and non-tsunami affected terrestrials. | the effects of tsunamis on microbial ecologies have been ill-defined, especially in phang nga province, thailand. this ecosystem was catastrophically impacted by the 2004 indian ocean tsunami as well as the 600 year-old tsunami in phra thong island, phang nga province. no study has been conducted to elucidate their effects on microbial ecology. this study represents the first to elucidate their effects on microbial ecology. we utilized metagenomics with 16s and 18s rdna-barcoded pyrosequencing t ... | 2014 | 24710002 |
| plasmid pupi126-encoded pyrrolnitrin production by acinetobacter haemolyticus a19 isolated from the rhizosphere of wheat. | an acinetobacter species identified as a. haemolyticus a19 produces an antibiotic and the enzyme chitinase. the antibiotic produced by a. haemolyticus a19 was extracellular and inducible by co-cultivation with klebsiella pneumoniae in the optimum ratio 2:1, respectively. ph 7, temperature 28 °c, and addition of 2% (w/v) nacl are the most suitable environmental conditions for production and activity of the antibiotic. the antibiotic was produced in the early stationary growth phase (48 h) of a. h ... | 2014 | 23990066 |
| identification and characterization of a cluster of genes involved in biosynthesis and transport of acinetoferrin, a siderophore produced by acinetobacter haemolyticus atcc 17906t. | acinetobacter haemolyticus atcc 17906(t) is known to produce the siderophore acinetoferrin under iron-limiting conditions. here, we show that an operon consisting of eight consecutive genes, named acbabcd and actbcad, participates in the biosynthesis and transport of acinetoferrin, respectively. transcription of the operon was found to be iron-regulated by a putative fur box located in the promoter region of the first gene, acba. homology searches suggest that acbabcd and acta encode enzyme prot ... | 2013 | 23378574 |
| characterization of novel diesel-degrading strains acinetobacter haemolyticus mj01 and acinetobacter johnsonii mj4 isolated from oil-contaminated soil. | the diesel-degrading strains, designated as mj01 and mj4, were isolated from oil-contaminated soil in daejeon (south korea) and were taxonomically characterized using a polyphasic approach and their diesel oil degradation abilities were analyzed. the isolates mj01 and mj4 were identified as acinetobacter haemolyticus and acinetobacter johnsonii, respectively, based on their 16s rdna gene sequences, dna-dna relatedness, fatty acid profiles and various physiological characteristics. strains mj01 a ... | 2012 | 22806027 |
| antioxidant, antimicrobial and antiverotoxic potentials of extracts of curtisia dentata. | there is an increase in antimicrobial resistance and complexities arising from verotoxic related bacterial infections as well as rise in demand for application of natural antioxidants to combat oxidative damage by free radicals in many oxidative stress-mediated disease conditions such as cancer. thus the potential of curtisia dentata as antimicrobial, antioxidant and antiverotoxin against environmental isolates of escherichia coli and acinetobacter spp. as well as the presence of phytochemicals ... | 2012 | 22504170 |
| isotherm kinetics of cr(iii) removal by non-viable cells of acinetobacter haemolyticus. | the potential use of non-viable biomass of a gram negative bacterium i.e. acinetobacter haemolyticus to remove cr(iii) species from aqueous environment was investigated. highest cr(iii) removal of 198.80 mg g(-1) was obtained at ph 5, biomass dosage of 15 mg cell dry weight, initial cr(iii) of 100 mg l(-1) and 30 min of contact time. the langmuir and freundlich models fit the experimental data (r(2)>0.95) while the kinetic data was best described using the pseudo second-order kinetic model (r(2) ... | 2012 | 22398363 |
| bacterial reduction of cr(vi) at technical scale--the malaysian experience. | the bacterial reduction of cr(vi) from industrial wastewater was evaluated using a 2.0-m(3) bioreactor. liquid pineapple waste was used as a nutrient for the biofilm community formed inside the bioreactor. the use of rubber wood sawdust as packing material was able to immobilize more than 10(6) cfu ml(-1) of acinetobacter haemolyticus cells after 3 days of contact time. complete reduction of 15-240 mg l(-1) of cr(vi) was achieved even after 3 months of bioreactor operation. cr(vi) was not detect ... | 2012 | 22350941 |
| molecular characterisation of carbapenemases in urban pigeon droppings in france and algeria. | the main objective of this study was to detect the presence of carbapenemase-encoding genes in stool samples of urban pigeons. | 2017 | 28487209 |
| maldi-tof ms for the identification of cultivable organic-degrading bacteria in contaminated groundwater near unconventional natural gas extraction sites. | groundwater quality and quantity is of extreme importance as it is a source of drinking water in the united states. one major concern has emerged due to the possible contamination of groundwater from unconventional oil and natural gas extraction activities. recent studies have been performed to understand if these activities are causing groundwater contamination, particularly with respect to exogenous hydrocarbons and volatile organic compounds. the impact of contaminants on microbial ecology is ... | 2017 | 28796186 |