| competition between beta-ketothiolase and citrate synthase during poly(beta-hydroxybutyrate) synthesis in methylobacterium rhodesianum. | the enzymes beta-ketothiolase and citrate synthase from the facultatively methylotrophic methylobacterium rhodesianum mb 126, which uses the serine pathway, were purified and characterized. the beta-ketothiolase had a relatively high km for acetyl-coa (0.5 mm) and was strongly inhibited by coa (ki 0.02 mm). the citrate synthase had a much higher affinity for acetyl-coa (km 0.07 mm) and was significantly inhibited by nadh (ki 0.15 mm). the intracellular concentration of coa metabolites and nucleo ... | 1996 | 9082918 |
| analysis of the pha granule-associated proteins ga20 and ga11 in methylobacterium extorquens and methylobacterium rhodesianum. | electrophoretic analysis of the proteins bound to poly(3-hydroxybutyric acid), phb-, granules in methylobacterium extorquens, m. rhodesianum as well as the phb-leaky mutants mu 1 and mu 11, which were isolated from the latter, resulted in two dominant low-molecular weight proteins, which were referred to as ga11 and ga20. after purification of these proteins antibodies against the ga11 and ga20 protein of m. extorquens were obtained. both proteins bound to the surface of phb granules as revealed ... | 1997 | 9090123 |
| regulation of poly(beta-hydroxybutyrate) synthesis in methylobacterium rhodesianum mb 126 growing on methanol or fructose. | the intracellular concentration of coa metabolites and nucleotides was determined in batch cultures of methylobacterium rhodesianum grown on methanol and shifted to growth on fructose. the intracellular concentration of coa decreased from a high value of 0.6 nmol/mg poly(beta-hydroxybutyrate)-free bacterial dry mass during growth on methanol to a low value of 0.03 nmol/mg poly(beta-hydroxybutyrate)-free bacterial dry mass after a shift to fructose as a carbon source. the levels of nadh, nadph, a ... | 1998 | 9531638 |
| methylobacterium populi sp. nov., a novel aerobic, pink-pigmented, facultatively methylotrophic, methane-utilizing bacterium isolated from poplar trees (populus deltoides x nigra dn34). | a pink-pigmented, aerobic, facultatively methylotrophic bacterium, strain bj001t, was isolated from internal poplar tissues (populus deltoidesxnigra dn34) and identified as a member of the genus methylobacterium. phylogenetic analyses showed that strain bj001t is related to methylobacterium thiocyanatum, methylobacterium extorquens, methylobacterium zatmanii and methylobacterium rhodesianum. however, strain bj001t differed from these species in its carbon-source utilization pattern, particularly ... | 2004 | 15280290 |
| characterization of micro-organisms isolated from dairy industry after cleaning and fogging disinfection with alkyl amine and peracetic acid. | to characterize micro-organisms isolated from norwegian dairy production plants after cleaning and fogging disinfection with alkyl amine/peracetic acid and to indicate reasons for survival. | 2005 | 15610421 |
| methylobacterium hispanicum sp. nov. and methylobacterium aquaticum sp. nov., isolated from drinking water. | members of the genus methylobacterium are ubiquitous in nature and can be isolated from almost any freshwater environment where dissolved oxygen exists. this genus is composed of a variety of pink-pigmented, facultatively methylotrophic (ppfm) bacteria. during a screening programme to monitor the bacterial population present in the drinking water of a municipal water supply in seville (spain) during the year 2003, five strains of ppfm bacteria were isolated and characterized. analysis of their c ... | 2005 | 15653888 |
| reclassification of methylobacterium chloromethanicum and methylobacterium dichloromethanicum as later subjective synonyms of methylobacterium extorquens and of methylobacterium lusitanum as a later subjective synonym of methylobacterium rhodesianum. | phylogenetic analysis based on 16s rdna sequences was performed on all type strains of the 14 validly described methylobacterium species to ascertain the genealogic relationships among these species. the results showed that type strains of methylobacterium were divided into two monophyletic groups whose members were distinct species with sequence similarity values greater than 97.0% between any two of the members in the same group. only m. organophilum jcm 2833(t) and atcc 27886(t) were not divi ... | 2005 | 16314683 |
| statistical modeling of dental unit water bacterial test kit performance. | while it is important to monitor dental water quality, it is unclear whether in-office test kits provide bacterial counts comparable to the gold standard method (r2a). studies were conducted on specimens with known bacterial concentrations, and from dental units, to evaluate test kit accuracy across a range of bacterial types and loads. | 2007 | 17508622 |
| production of the chiral compound (r)-3-hydroxybutyrate by a genetically engineered methylotrophic bacterium. | in this study, a methylotrophic bacterium, methylobacterium rhodesianum mb 126, was used for the production of the chiral compound (r)-3-hydroxybutyrate (r-3hb) from methanol. r-3hb is formed during intracellular degradation of the storage polymer (r)-3-polyhydroxybutyrate (phb). since the monomer r-3hb does not accumulate under natural conditions, m. rhodesianum was genetically modified. the gene (hbd) encoding the r-3hb-degrading enzyme, r-3hb dehydrogenase, was inactivated in m. rhodesianum. ... | 2010 | 20581197 |
| inter individual variations of the fish skin microbiota: host genetics basis of mutualism? | the commensal microbiota of fish skin is suspected to provide a protection against opportunist infections. the skin of fish harbors a complex and diverse microbiota that closely interacts with the surrounding water microbial communities. up to now there is no clear evidence as to whether the host regulates the recruitment of environmental bacteria to build a specific skin microbiota. to address this question, we detected quantitative trait loci (qtl) associated with the abundance of specific ski ... | 2014 | 25068850 |
| [isolation and degradation characteristics of dichloromethane-degradation bacterial strain by methylobacterium rhodesianum h13]. | a dichloromethane-degrading bacterium methylobacterium rhodesianum h13 which utilized the dcm as the sole carbon and energy source was isolated. according to the research, m. rhodesianum h13 could completely degrade 5 mmol x l(-1) dcm in 23 h with the initial cell concentration of 0.82 mg x l(-1), ph 7.0, 30 degrees c, and the cell yield rate was about 0.136 g x g(-1) dcm. with the degradation of dcm, cl- concentration gradually raised (the release of cl- concentration was about 2 times higher a ... | 2013 | 24289013 |
| effective utilization of dichloromethane by a newly isolated strain methylobacterium rhodesianum h13. | an effective dichloromethane (dcm) utilizer methylobacterium rhodesianum h13 was isolated from activated sludge. a response surface methodology was conducted, and the optimal conditions were found to be 4.5 g/l na2hpo4·12h2o, 0.5 g/l (nh4)2so4, an initial ph of 7.55, and a temperature of 33.7 °c. the specific growth rate of 0.25 h(-1) on 10 mm dcm was achieved, demonstrating that m. rhodesianum h13 was superior to the other microorganisms in previous investigations of dcm utilization. dcm minera ... | 2014 | 23856743 |