| characterization and identification of coagulase-negative, heat-stable deoxyribonuclease-positive staphylococci. | various characteristics of 13 coagulase-negative, weakly heat-stable deoxyribonuclease-positive staphylococci from human, veterinary and food sources were determined in an effort to identify them. nine of the isolates were identified as coagulase-negative staphylococcus aureus (2), staphylococcus xylosus (2), staphylococcus simulans (3), staphylococcus capitis (1) and staphylococcus sciuri subsp. lentus (1); the other four isolates, from food and veterinary sources, could not be identified as cu ... | 1978 | 641525 |
| multiple binding sites in fibronectin and the staphylococcal fibronectin receptor. | the binding of fibronectin to staphylococci exhibits the properties of a ligand-receptor interaction and has been proposed to mediate bacterial adherence to host tissues. to localize staphylococcal-binding sites in fibronectin, the protein was subjected to limited proteolysis and, of the generated fragments, staphylococci appeared to preferentially bind to the n-terminal fragment. different fibronectin fragments were isolated and tested for their ability to inhibit 125i-fibronectin binding to st ... | 1992 | 1385780 |
| risk of bacteremia induced by transesophageal echocardiography: analysis of 100 consecutive procedures. | the incidence of bacteremia induced by transesophageal echocardiography (tee) and, consequently, the need for an antibiotic prophylaxis before tee is still controversial. therefore, we studied the incidence of bacteremia associated with tee prospectively in 100 consecutive patients without clinical or laboratory signs of bacterial infection. blood samples were drawn immediately before and at 0, 5, and 15 minutes after tee. in addition, swabs were taken from the pharyngeal region before tee and f ... | 1992 | 1571172 |
| staphylococcus capitis endocarditis: a new cause of an old disease. | | 1992 | 1571458 |
| infective endocarditis due to staphylococcus capitis. | | 1992 | 1617060 |
| identification of coagulase-negative staphylococci by using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and rrna restriction patterns. | a total of 1,417 staphylococcal and micrococcal strains were collected from the beards and scalps of 10 subjects over a period of 8 months. sixteen strains identified as staphylococcus epidermidis with an api system had distinctive yellow colonies on nutrient agar plates and sodium dodecyl sulfate-polyacrylamide gel electrophoresis whole-cell polypeptide profiles similar to those of staphylococcus capitis; this identification was confirmed by analysis of rrna gene restriction patterns. | 1991 | 1706732 |
| staphylococcus capitis subsp. ureolyticus subsp. nov. from human skin. | a new subspecies, staphylococcus capitis subsp. ureolyticus, was isolated from human skin and is described on the basis of studies of 15 to 26 strains. dna-dna reassociation reactions demonstrated that these strains were closely related to staphylococcus capitis but were significantly divergent. the strains of s. capitis subsp. ureolyticus can be distinguished from s. capitis by their positive urease activity, their ability to produce acid from maltose under aerobic conditions, their fatty acid ... | 1991 | 1995030 |
| isolation and identification of staphylococci from milk powders produced in northern ireland. | milk powders (37 samples) from five different processing centres (a, b, c, d and e) were examined for total viable counts, total staphylococcal counts and staphylococcal enterotoxins. all powders from centres a, b and c contained low numbers of total viable bacteria and staphylococci but five from centres d and e had high total and staphylococcal counts. nine different staphylococcal species were encountered in low count powders with a wide range of species occurring at each of the five centres. ... | 1990 | 2370231 |
| temporal study of staphylococcal species on the skin of human subjects in isolation and clonal analysis of staphylococcus capitis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. | the staphylococcal skin floras of an isolated group of subjects were studied for 1 year. a wide variation in isolation patterns was found for different species. staphylococcus intermedius, previously thought to be of veterinary origin, was found to be part of the resident flora of some subjects, and this may indicate a wider role for it in clinical infection. sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) analysis of s. capitis isolates indicated persistent skin colonizatio ... | 1989 | 2687313 |
| effect of milk on fibronectin and collagen type i binding to staphylococcus aureus and coagulase-negative staphylococci isolated from bovine mastitis. | tryptic soy broth (tsb)-grown cells of staphylococcus aureus isolated from acute and chronic bovine mastitis bound mainly 125i-fibronectin (fn) [corrected], whereas strains of nine species of coagulase-negative staphylococci showed a predominant interaction with 125i-collagen (cn) [corrected] type i. a particle agglutination assay (paa) was used to examine the interaction of coagulase-negative staphylococci with 125i-fn and 125i-cn immobilized on latex. all 368 coagulase-negative staphylococci d ... | 1989 | 2775349 |
| serum hdl2 distribution in patients with coronary heart disease and controls as estimated by a precipitation reaction between hdl2 and an extract of staphylococcus capitis. | serum hdl2 concentration has been shown to be lowered in patients with coronary heart disease (chd). since we previously reported that serum hdl2 can be estimated by single radial diffusion in agar containing an extract of staphylococcus capitis, it seemed of interest to study whether serum hdl2 as estimated by this method is different in chd patients and controls. it was shown that relative activity of hdl2, assessed by the method, was appreciably lower in 40 men with angiographically proven co ... | 1989 | 2803754 |
| properties of a protein from staphylococcus capitis that binds human serum high density lipoprotein. | a high density lipoprotein (hdl) binding component from the cell wall of staphylococcus capitis, possessing both hdl binding and hdl precipitating activity, has been partially characterised. growth of the bacteria on medium containing cysteine or in presence of co2, was found to induce the synthesis of this factor. analysis show an incorporation of radioactive amino acids into the factor, and also its sensitivity to trypsin, indicating strongly that the hdl binding factor is of protein nature. g ... | 1988 | 3213315 |
| evidence that an extract of staphylococcus capitis can precipitate human serum hdl2. | the high density lipoprotein (hdl) fraction involved in the precipitation with staphylococcus capitis can be quantitated by single radial diffusion in agar containing an extract of the bacteria. both whole serum and purified hdl produce a precipitation ring with bacterial extract. when dilutions of serum are tested, the squared radius of the precipitation ring plotted against serum concentration gives a linear relationship. cumulative density flotation of hdl, followed by quantitative analysis o ... | 1986 | 3565014 |
| binding of human serum high density lipoprotein to staphylococcus capitis. | of twelve different staphylococcal species tested, staphylococcus capitis and staphylococcus warneri bound human serum high density lipoprotein (hdl). the binding of hdl was observed with bacteria cultivated of brain-heart infusion broth agar, but not on nutrient broth agar. the factor mediating the binding of hdl seems to be a bacterial component and not a component of the growth medium. studies on the binding of hdl to s. capitis showed that a subfraction of hdl was involved in the reaction. t ... | 1985 | 3936335 |
| [infection of the valves of csf shunts. results of local and general antibiotic treatment in 6 cases]. | infection of six cerebro-spinal fluid shunts in six patients were treated with daily intrashunt injections of antibiotics plus combination of intravenous or oral systemic antibiotic therapy. three organisms were involved in the shunt infection: staphylococcus epidermidis in five, staphylococcus capitis and micrococcus varians. one patient had mixed shunt infection with micrococcus varians and staphylococcus épidermidis. all six patients received daily intrashunt injections of vancomycin and oral ... | 1985 | 4088406 |
| [drug susceptibility of staphylococci isolated from clinical specimens]. | eighty-eight strains of staphylococci were isolated from a variety of specimens collected from patients at our university hospital from june to august 1982. these strains were identified as staphylococcus aureus (86%), staphylococcus epidermidis (6.8%), staphylococcus haemolyticus (2.3%), staphylococcus capitis (2.3%), and staphylococcus simulans (1.1%). one strain could not be identified with certainty as a currently recognized species. a majority (70%) of the s. aureus strains was resistant to ... | 1985 | 4089343 |
| expression of the gene encoding protein a in staphylococcus aureus and coagulase-negative staphylococci. | two shuttle vectors containing the gene for protein a (spa) from staphylococcus aureus have been constructed to study expression of the gene in various strains of s. aureus and in the coagulase-negative species staphylococcus epidermidis, staphylococcus capitis, and staphylococcus xylosus. one plasmid, pspa15, contains the complete structural gene for protein a, which binds to the cell wall in various staphylococcus species. the other plasmid, pspa16, codes for a truncated protein a lacking the ... | 1984 | 6086584 |
| identification, clinical distribution, and susceptibility to methicillin and 18 additional antibiotics of clinical staphylococcus isolates: nationwide investigation in italy. | a multicentric study of clinical staphylococcus isolates was performed by seven operative units working in different areas of italy. over a 6-month period, a total of 3,226 staphylococci, isolated from in- and outpatients, were identified and tested for antimicrobial susceptibility by a protocol agreed upon by all units. on the basis of their bacteriolytic-activity patterns and other conventional tests, the isolates were identified by lyogroups , which closely correlate with human staphylococcus ... | 1984 | 6565709 |
| staphylococci producing a non-immune precipitation line with human serum. | when grown on brain heart agar plates, staphylococcus capitis, s. warneri and s. xylosus produced a precipitation line with normal human serum in the double diffusion test. eight other staphylococcal species did not produce this line. of 20 strains of s. capitis, all produced the precipitation line, and the type strain of s. capitis produced the precipitation line against the sera from eight animal species. the precipitation is not caused by protein a and it is not an immune reaction because the ... | 1981 | 6789609 |
| the effect of benzoyl peroxide on cutaneous micro-organisms in vitro. | the survival curves of cutaneous micro-organisms in the presence of benzoyl peroxide were investigated. all the curves exhibited a shoulder prior to exponential cell death. benzoyl peroxide was lethal to the cutaneous organisms tested and they varied in sensitivity increasing as follows: propionibacterium acnes, staphylococcus capitis, staph. epidermidis, staph. hominis, prop. avidum, prop. granulosum and pityrosporum ovale. | 1983 | 6874624 |
| properties of the serum factor involved in the precipitation reaction with staphylococcus capitis. | the serum factor was precipitated by both rivanol and ammonium sulphate, and it bound to both deae-cellulose and blue-sepharose cl-6b from which it subsequently could be eluted. immunoelectrophoresis showed that the serum factor had alpha 1-mobility, and isoelectric focusing indicated an apparent isoelectric point of 5.2. purification by ultracentrifugation, followed by agarose chromatography, showed that the serum factor is present in the high density lipoprotein fraction of serum. | 1982 | 7124404 |
| speciation and antibiotic susceptibility patterns of coagulase-negative staphylococci. | during a six month period, 191 isolates of coagulase-negative staphylococci from blood, urine, cerebrospinal fluid and heart valves were identified to species level and tested for antimicrobial susceptibility. seventy-one percent of isolates were staphylococcus epidermidis, 8% staphylococcus warneri, 7% staphylococcus hominis, 7% staphylococcus haemolyticus, 4% staphylococcus capitis, 2% staphylococcus saprophyticus and 1% staphylococcus cohnii. approximately 4% of isolates were felt to be assoc ... | 1982 | 7173185 |
| polynucleotide sequence divergence among some coagulase-negative staphylococci. | the degree of binding was determined, at both exacting and non-exacting temperatures, between deoxyribonucleic acid (dna) preparations from coagulase-negative staphylococci and carbon (14c) labelled dna from nine reference strains, staphylococcus capitis lk499, s. cohnii gh137, s. epidermidis gh37, s. haemolyticus sm131, s. hominis dm122, s. saprophyticus ccm883, s. simulans sm128, s. warneri aw25 and s. xylosus kl162. the close similarity in nucleotide sequences found between five of the six re ... | 1980 | 7424234 |
| antistaphylococcal activity of cefdinir, a new oral third-generation cephalosporin, alone and in combination with other antibiotics, at supra- and sub-mic levels. | cefdinir is one of the few oral third generation cephalosporins that shows useful activity against nosocomial gram-positive pathogens. for this reason the anti-staphylococcal potency of the new drug, alone or in combination with other drugs was further characterized. against penicillin-resistant, oxacillin-susceptible staphylococcus isolates, cefdinir demonstrated useful in-vitro activity. mic90 values (in mg/l) were 0.25 for staphylococcus aureus (30 strains), 0.06 for staphylococcus epidermidi ... | 1995 | 7768782 |
| in vitro modulation of keratinocyte-derived interleukin-1 alpha (il-1 alpha) and peripheral blood mononuclear cell-derived il-1 beta release in response to cutaneous commensal microorganisms. | the ability of a range of skin commensal microorganisms to modulate interleukin-1 (il-1) release by cultured human keratinocytes and peripheral blood mononuclear cells (pbmcs) was investigated by a combination of enzyme-linked immunosorbent assays and bioassays. three fractions (formaldehyde-treated whole cells, culture supernatants, and cellular fractions) were prepared from propionibacterium acnes, propionibacterium granulosum, staphylococcus epidermidis, staphylococcus capitis, staphylococcus ... | 1995 | 7890376 |
| immunological recognition of fibronectin-binding proteins of staphylococcus aureus and staphylococcus capitis, strain lk 499. | antibodies to fibronectin-binding proteins (fnbps) of staphylococcus aureus, including binding domain of fnbpa, the d region, or the a-c regions of fnbpb were produced in rabbits and mice. these antibodies were used to characterize cell-associated fnbps of s. aureus strain cowan i, s. aureus strain u320 and a coagulase-negative staphylococcus capitis strain lk499 as well as extracellular fnbps in culture supernatants of the strain u320. fnbps of s. aureus were predominantly fnbpa, while fnbpb wa ... | 1994 | 7935060 |
| activation of the human immunodeficiency virus long terminal repeat in thp-1 cells by a staphylococcal extracellular product. | staphylococcal strains can release a factor that strongly activates the human immunodeficiency virus type 1 (hiv-1) long terminal repeat (ltr) in thp-1 cells transfected with the hiv-1 ltr-driven luciferase reporter gene (thp-1 ltrluc). the factor is present in the overnight culture fluid and is readily released from the organisms into aqueous medium by vigorous mixing. staphylococcal extracellular material is a complex mixture of polysaccharide and protein containing peptidoglycan and teichoic ... | 1994 | 7938001 |
| characterization of the most frequently encountered staphylococcus sp. in cats. | ninety three staphylococci isolated from clinical specimens from cats were characterized and identified. because the biochemical characteristics of staphylococcus felis were very similar to those of staphylococcus simulans, results were submitted to numerical analysis and dna homology. forty-two isolates (45%) were identified as s. felis, and 4 isolates (4%) as s. simulans. the other species identified, in order of their frequency were, 12 staphylococcus aureus (13%), 9 staphylococcus intermediu ... | 1994 | 8042273 |
| comparison of the smai-digested chromosomes of staphylococcus epidermidis and the closely related species staphylococcus capitis and staphylococcus caprae. | pulsed-field gel electrophoresis was used to examine the chromosomal polymorphisms existing within and between four closely related members of the staphylococcus epidermidis species group, s. epidermidis, staphylococcus caprae, staphylococcus capitis subsp. capitis, and s. capitis subsp. ureolyticus. smai was chosen as the restriction endonuclease for this study because it generated only a few well-separated chromosomal fragments. each of the species and subspecies showed distinct smai digest pa ... | 1994 | 8068539 |
| native valve endocarditis caused by staphylococcus capitis. | | 1993 | 8307053 |
| endocarditis due to staphylococcus capitis subspecies ureolyticus. | | 1993 | 8443328 |
| prosthetic valve endocarditis caused by staphylococcus capitis. | | 1996 | 8678683 |
| a simple and inexpensive method for the identification of staphylococcus epidermidis and staphylococcus hominis. | eight hundred and ninety-two strains of staphylococcus species were identified by means of desferrioxamine susceptibility and fermentation results of three carbohydrates, with the api staph system (biomérieux, france) as reference method. no identification could be obtained for 34 strains with api staph. of the remaining 858 strains, identical identification was obtained with 842 (98.1%). all 707 strains identified as staphylococcus epidermidis or staphylococcus hominis by the api staph system w ... | 1995 | 8681979 |
| coagulase-negative staphylococci in danish blood cultures: species distribution and antibiotic susceptibility. | the distribution and antibiotic susceptibility of coagulase-negative staphylococci (cons) isolated from blood cultures was examined in samples from hospitals covering most of denmark. a total of 499 cons isolates were detected in 477 blood cultures from 340 patients and speciated as staphylococcus epidermidis, 285; staphylococcus hominis, 61; staphylococcus haemolyticus, 43; staphylococcus warneri, 12; staphylococcus cohnii, 7; staphylococcus saprophyticus, 4; staphylococcus capitis, 2 and staph ... | 1996 | 8690885 |
| susceptibility of transient and commensal skin flora to the essential oil of melaleuca alternifolia (tea tree oil). | the purpose of this study was to determine the susceptibility of a range of transient and commensal skin flora to the essential oil of melaleuca alternifolia, or tea tree. | 1996 | 8806995 |
| predictive values of species' identifications from the vitek gram-positive identification card using clinical isolates of coagulase-negative staphylococci. | the gram-positive identification card (gpi, biomerieux vitek) was compared to conventional tests for identification of 616 clinical isolates of coagulase-negative staphylococci. all tests were inoculated with subcultures from single isolated colonies. the predictive values of gpi species' identifications (the number of the system's correct calls divided by the total number of calls for each species) were 100% (12 of 12) for staphylococcus capitis, 100% (79 of 79) for s saprophyticus, 98.4% (246 ... | 1996 | 8816597 |
| purification and molecular characterization of glycylglycine endopeptidase produced by staphylococcus capitis epk1. | a novel staphylolytic enzyme, ale-1, acting on staphylococcus aureus, was purified from a staphylococcus capitis epk1 culture supernatant. the optimal ph range for staphylolytic activity was 7 to 9. ale-1 contains one zn2+ atom per molecule. analysis of peptidoglycan fragments released by ale-1 indicated that the enzyme is a glycylglycine endopeptidase. the effects of various modulators were determined, and we found that o-phenanthroline, iodoacetic acid, diethylpyrocarbonate, and cu2+ reduced t ... | 1997 | 9023202 |
| molecular cloning, sequencing, and expression of lytm, a unique autolytic gene of staphylococcus aureus. | a gene encoding an autolytic activity was identified in an autolysis-deficient mutant (lyt-) of staphylococcus aureus which produces only a single band in autolytic-activity gels (n. mani, p. tobin, and r. k. jayaswal, j. bacteriol. 175:1493-1499, 1993). an open reading frame, designated lytm, of 948 bp that could encode a polypeptide of 316 amino acid residues was identified. the calculated molecular mass of the lytm gene product (34.4 kda) corresponded to that of the autolytic activity detecte ... | 1997 | 9171409 |
| epr, which encodes glycylglycine endopeptidase resistance, is homologous to femab and affects serine content of peptidoglycan cross bridges in staphylococcus capitis and staphylococcus aureus. | staphylococcus capitis epk1 produces a glycylglycine endopeptidase, ale-1 (m. sugai, t. fujiwara, t. akiyama, m. ohara, h. komatsuzawa, s. inoue, and h. suginaka, j. bacteriol. 179:1193-1202, 1997), which hydrolyzes interpeptide pentaglycine chains of cell wall peptidoglycan of s. aureus. characterizations of the enzyme activity and cloning of ale-1 revealed that ale-1 is very similar to prolysostaphin produced by s. simulans bv. staphylolyticus. strain epk1 is resistant to lysis by ale-1 and by ... | 1997 | 9209049 |
| infective endocarditis associated with upper endoscopy: case report and review. | we describe a 73-year-old male who developed native valve infective endocarditis due to staphylococcus capitis as a consequence of repeated oesophageal dilatation. the patient was treated with appropriate antibiotic therapy but his condition deteriorated and he died. we review 10 previously reported cases of infective endocarditis associated with upper endoscopy and discuss the issue of prophylaxis of high risk patients prior to upper endoscopy involving various invasive procedures, e.g. biopsy, ... | 1998 | 9661953 |
| staphylococcus capitis causing aortic valve endocarditis. | coagulase-negative staphylococci have been implicated in certain human infections but are generally considered to be contaminants. although their clinical significance has been questioned, rare species of coagulase-negative staphylococci such as staphylococcus capitis and staphylococcus ludgunensis have recently emerged which cause endocarditis with high morbidity and mortality rates. we report a case of severe aortic valve endocarditis with conduction delays due to s. capitis infection which wa ... | 1998 | 9793849 |
| possible implication of sterile connecting device in contamination of pooled platelet concentrates. | considering the possibility that a pooled random donor platelet concentrate could become contaminated by welding with a sterile connecting device, we undertook a study to determine the influence of pooling on the contamination rate. as a control group, apheresis platelets were examined. bacteriological testing was done with a sensitive co2 detecting culture system, the bact/ alert. out of 1105 pooled platelet concentrates prepared by the buffy coat method, 15 (1.4%) were confirmed as contaminate ... | 1997 | 10175151 |
| [infectious endocarditis of prosthetic valves due to staphylococcus capitis: a new case]. | we present the case of a male patient with aortic and mitral valve bioprostheses who developed infectious endocarditis due to staphylococcus capitis, which has recently been described as an agent producing infectious endocarditis in native and prosthetic cardiac valves. the patient's course evolved unfavorably, despite specific antibiotic treatment, leading to the surgical replacement of the valve, which completely resolved the problem. this case points out that, although rare, in infectious end ... | 1999 | 10217971 |
| species identification and phylogenetic relationships based on partial hsp60 gene sequences within the genus staphylococcus. | the phylogenetic relationships among 36 validly described species or subspecies within the genus staphylococcus were investigated by cloning and sequencing their 60 kda heat-shock protein (hsp60) genes using a set of universal degenerate hsp60 pcr primers. the cloned partial hsp60 dna sequences from nine staphylococcus aureus strains were highly conserved (97-100% dna sequence similarity; mean 98%), indicating that the hsp60 gene of multiple isolates within the same species have little microhete ... | 1999 | 10425778 |
| staphylococcus capitis endocarditis: two cases and review of the literature. | coagulase negative staphylococci are the principal cause of prosthetic valve endocarditis but are a rare cause of native valve infections. however, the incidence of native valve endocarditis is increasing. staphylococcus capitis is a coagulase negative staphylococcus with the capacity to cause endocarditis on native heart valves. two cases of native valve endocarditis caused by s capitis are presented; both in patients with aortic valve disease. the patients were cured with prolonged intravenous ... | 1999 | 10455099 |
| randomized, double-blind trial of an antibiotic-lock technique for prevention of gram-positive central venous catheter-related infection in neutropenic patients with cancer. | the aim of the present study was to determine the efficacy of an antibiotic-lock technique in preventing endoluminal catheter-related infection with gram-positive bacteria in neutropenic patients with hematologic malignancies. patients with nontunneled, multilumen central venous catheters were assigned in a randomized, double-blinded manner to receive either 10 u of heparin per ml (57 patients) or 10 u of heparin per ml and 25 microg of vancomycin per ml (60 patients), which were instilled in th ... | 1999 | 10471564 |
| bloodstream infections caused by small-colony variants of coagulase-negative staphylococci following pacemaker implantation. | small-colony variants (scvs) of staphylococcus aureus cause persistent and relapsing infections. relatively little is known regarding infections caused by scvs of coagulase-negative staphylococci. we report two cases of pacemaker electrode infections due to scvs of staphylococcus epidermidis and staphylococcus capitis. sequence analysis of a portion of the 16s rrna gene (16s rdna) confirmed the identity of the staphylococcal species as s. capitis and s. epidermidis. isolates from cultures of blo ... | 1999 | 10589914 |
| site-specific serine incorporation by lif and epr into positions 3 and 5 of the staphylococcal peptidoglycan interpeptide bridge. | the femab-like factors lif and epr confer resistance to glycylglycine endopeptidases lysostaphin and ale-1, respectively, by incorporating serine residues into the staphylococcal peptidoglycan interpeptide bridges specifically at positions 3 and 5. this required the presence of fema and/or femb, in contrast to earlier postulations. | 2000 | 10762270 |
| rapid identification of staphylococcus epidermidis. | during the collection of airborne bacteria in a museum in england some bacterial strains were isolated which due to their fatty acid profiles were clearly identified as members of the genus staphylococcus. as fatty acid compositions of coagulase-negative staphylococci are very similar, differing only in quantities but not in qualities, further identification at the species level without a fatty acid database was not achieved. investigation of the isolates using the staph id 32 api system resulte ... | 2000 | 10843049 |
| bacterial response to acetate challenge: a comparison of tolerance among species. | although acetate formation and tolerance are important criteria for various aspects of biotechnological process development, available studies on acetate tolerance in different species are disparate. we evaluate the response of eight bacterial strains, including two variants of escherichia coli, two variants of staphylococcus capitis, and one each of acetobacter aceti, gluconobacter suboxydans, lactobacillus acetotolerans, and l. bulgaricus, to acetate challenges under identical conditions. our ... | 2000 | 10968640 |
| aortic valve endocarditis due to staphylococcus capitis. | native valve endocarditis due to staphylococcus capitis is uncommon and is usually managed conservatively with good outcome. of the nine previously reported cases there has been only one mortality. we report a case of native aortic valve endocarditis due to s capitis in an elderly diabetic that had a fatal outcome despite appropriate therapy with antibiotics. a review the literature is also presented. | 2000 | 11107206 |
| bacterial flora of the conjunctiva and nasal cavity in normal and diseased captive bustards. | a survey was carried out to describe the normal aerobic bacterial flora of the conjunctiva and nasal cavity of captive houbara bustards (chlamydotis undulata), kori bustards (ardeotis kori), and white-bellied bustards (eupodotis senegalensis) maintained at the national avian research center, abu dhabi, united arab emirates. a total of 58 samples were examined from the nasal cavity and 55 samples from the conjunctiva of healthy bustards. there was no bacterial growth in 45% of conjunctival sample ... | 2001 | 11417826 |
| molecular microbial diversity of a spacecraft assembly facility. | in ongoing investigations to map and archive the microbial footprints in various components of the spacecraft and its accessories, we have examined the microbial populations of the jet propulsion laboratory's spacecraft assembly facility (jpl-saf). witness plates made up of spacecraft materials, some painted with spacecraft qualified paints, were exposed for approximately 7 to 9 months at jpl-saf and examined the particulate materials collected for the incidence of total cultivable aerobic heter ... | 2001 | 11518337 |
| presence of propionibacterium acnes in blood components. | sterility testing, as part of the qc of blood components at the japanese red cross aichi blood center between april 1998 and march 2000, showed that 10 of 5568 tested blood components (0.18%), all of which were rbc concentrates, were contaminated with bacteria. nine isolates were propionibacterium acnes and one was staphylococcus capitis. | 2001 | 11552069 |
| staphylococcus capitis bacteremia of very low birth weight premature infants at neonatal intensive care units: clinical significance and antimicrobial susceptibility. | coagulase-negative staphylococci (cns) are frequently isolated from blood cultures in critically ill neonates. however, staphylococcus capitis is rarely reported as a pathogen in human beings. from january, 1995 to december, 1997 at a tertiary care neonatal intensive care unit (nicu), a total of 147 (62%) cns isolates were detected from 236 positive blood cultures, including 27 isolates of s. capitis. among the s. capitis bacteremia, 17 isolates were judged to be infections as opposed to 10 of t ... | 1999 | 11561567 |
| nosocomial spread of a staphylococcus capitis strain with heteroresistance to vancomycin in a neonatal intensive care unit. | a premature infant in a neonatal intensive care unit (nicu) developed a bloodstream infection caused by coagulase-negative staphylococci (cons) sensitive to vancomycin. the infection persisted for 3 weeks, despite therapy with vancomycin and replacement of all intravenous catheters. the neonate died due to necrotizing enterocolitis which developed during the ongoing sepsis. we screened this strain and 216 other strains of cons from cultures of blood obtained from neonates between 1997 and 2000 f ... | 2002 | 12089273 |
| microbiology of acute otitis externa. | to isolate and characterize bacteria and fungi from acute otitis externa (aoe) and to obtain susceptibility profiles on each bacterial isolate. | 2002 | 12169893 |
| aerobic salivary bacteria in wild and captive komodo dragons. | during the months of november 1996, august 1997, and march 1998, saliva and plasma samples were collected for isolation of aerobic bacteria from 26 wild and 13 captive komodo dragons (varanus komodoensis). twenty-eight gram-negative and 29 gram-positive species of bacteria were isolated from the saliva of the 39 komodo dragons. a greater number of wild than captive dragons were positive for both gram-negative and gram-positive bacteria. the average number of bacterial species within the saliva o ... | 2002 | 12238371 |
| fatal bacteria granuloma after trauma: a new entity. | in the past 20 years, more than 20 cases of a type of granulomatous disease have been noticed by dermatologists in different areas of china. the patients had these features in common: (i) the lesions followed a slight trauma to the face; (ii) they were spreading dark-red plaques without pus or ulceration; (iii) new lesions appeared near to or far from the original lesion; (iv) histopathology showed histiocytic granuloma; (v) the patients had severe headache and clouding of consciousness during t ... | 2002 | 12410712 |
| occurrence of resistance to antibiotics, metals, and plasmids in clinical strains of staphylococcus spp. | presence of staphylococci resistant to antibiotics is of concern, as is the possible spread of resistance determinants among staphylococcus species. during the past several years, a series of staphylococcal isolates that demonstrated reduced susceptibility to vancomycin or other glycopeptides has been reported. however, there are no useful data on species sensitivity of coagulase-negative staphylococci to heavy metals. | 2003 | 12700009 |
| overexpression of the atp-dependent helicase recg improves resistance to weak organic acids in escherichia coli. | increased resistance to several weak organic acids was conferred on escherichia coli by overexpression of the atp-dependent helicase recg and, to a lesser extent, by overexpressing the helicase ruvab. this property of helicases was identified by reproducible selection of recg-bearing clones from genomic libraries of the acetate-resistant species acetobacter aceti and staphylococcus capitis. we show that overexpression of recg from both species, but also from e. coli, increased the maximum biomas ... | 2003 | 12898065 |
| pcr-based identification of staphylococcus epidermidis targeting gsea encoding the glutamic-acid-specific protease. | the frequency of the gsea gene encoding a glutamic acid-specific serine protease, gluse, of staphylococcus epidermidis was investigated. dna hybridization analysis demonstrated that gsea existed exclusively in s. epidermidis but not in other bacteria examined. a single step pcr assay with a set of designed primers yielded amplification of gsea from all 69 clinical isolates of s. epidermidis taken from patients and healthy adults, whereas production of gluse was observed in 74% (51/69) of the iso ... | 2004 | 15381974 |
| sdrx, a serine-aspartate repeat protein expressed by staphylococcus capitis with collagen vi binding activity. | staphylococcus capitis (s. capitis) has been implicated in a large proportion of coagulase-negative staphylococcal infections in very-low-birth-weight infants. to identify potential therapeutic targets, the s. capitis genome was probed for the presence of genes encoding microbial surface components recognizing adhesive matrix molecules (mscramm). by using southern blot analysis, an s. capitis gene, designated sdrx, that contained sequence motifs consistent with the sdr family of mscramm proteins ... | 2004 | 15501749 |
| interactions in biofilms between listeria monocytogenes and resident microorganisms from food industry premises. | twenty nine bacterial strains were grown as binary culture biofilms with listeria monocytogenes to assess their influence on the settlement of the latter on stainless steel coupons. most of the strains had been isolated from food processing plants after cleaning and disinfection and were tentatively identified by the apilab plus 3.3.3 database (biomerieux). sixteen of them decreased l. monocytogenes biofilm colony forming units (cfu) counts. three strains, bacillus sp. ccl 9 an unidentified gram ... | 2004 | 15541798 |
| mutation analysis of the histidine residues in the glycylglycine endopeptidase ale-1. | a novel staphylolytic enzyme, ale-1, is a glycylglycine endopeptidase produced by staphylococcus capitis epk1. ale-1 possesses seven histidines. chemical modification studies using diethylpyrocarbonate and iodoacetic acid suggested that a histidine or tyrosine residue(s) in the molecule is important for the organism's staphylolytic activity. all of the histidine residues, one tyrosine, and one aspartic acid residue in the n-terminally truncated ale-1 (deltan-term ale-1) were systematically alter ... | 2005 | 15629919 |
| identification of coagulase-negative staphylococci other than staphylococcus epidermidis by automated ribotyping. | as routine identification of coagulase-negative staphylococci is problematic, the performance of automated ribotyping was evaluated for identification of coagulase-negative staphylococci other than staphylococcus epidermidis. in total, 177 isolates were tested, comprising 149 isolates from blood samples, 15 isolates that were not identified by internal transcribed spacer (its)-pcr in a previous study, and 13 reference strains. the identification results were compared with those obtained by the a ... | 2005 | 15715714 |
| plasmid-specified femabx-like immunity factor in staphylococcus sciuri dd 4747. | a plasmid from staphylococcus sciuri dd 4747 had three open reading frames: a replication gene, an n-acetylmuramyl-l-alanine amidase-like gene, and a gene similar to the lysostaphin endopeptidase resistance gene (epr/lif). the epr-like gene was introduced into s. aureus rn4220; the recombinant strain was more resistant to lysostaphin endopeptidase and its cell wall peptidoglycan contained more serines and fewer glycines than the parental strain with the shuttle vector alone. based on both its fu ... | 2005 | 16006076 |
| staphylococcus capitis endocarditis due to a transvenous endocardial pacemaker infection: case report and review of staphylococcus capitis endocarditis. | newer microbiologic methods to determine the species of coagulase-negative staphylococci (cons) have evolved which have shown that most endocarditis due to cons is caused by staphylococcus epidermidis, and far fewer by staphylococcus warneri and staphylococcus lugdunensis. | 2005 | 16085441 |
| coagulase-negative staphylococci in very-low-birth-weight infants: inability of genetic markers to distinguish invasive strains from blood culture contaminants. | selected coagulase-negative staphylococci from the blood of very-low-birth-weight infants in the neonatal intensive care unit at the royal women's hospital, melbourne, collected over a 5-year period were examined. isolates were classified as invasive or contaminants, speciated, typed by pulsed-field gel electrophoresis, and examined for biofilm genes (icaa, icac, and icad), adhesion genes (atle, fbe), and the number of copies of is256. of the 24 isolates studied, there were 13 contaminants and 1 ... | 2006 | 16598472 |
| urease-positive bacteria other than helicobacter pylori in human gastric juice and mucosa. | many bacteria carry the urease enzyme in different human ecosystems, but helicobacter pylori is the only known bacterium showing urease activity in gastric ecosystems. for this reason, the rapid urease test (rut) on gastric biopsies and urea breath test (c-ubt) are used to detect h. pylori infection. the aim of this study was to evaluate the presence of urease-positive bacteria other than h. pylori in gastric juice and mucosa in hypochlorhydric subjects. | 2006 | 16780553 |
| persistent staphylococcus capitis septicemia in a preterm infant. | a preterm infant had persistent staphylococcus capitis septicemia with 11 consecutive positive blood cultures over a period of 33 days. the clinical evidence suggested that the source of infection probably originated from the gastrointestinal tract. the combination of rifampin and linezolid treatment, together with prolonged stoppage of enteral feeding, successfully terminated the infection. rifampin and linezolid should be considered as alternative antimicrobial agents when glycopeptides fail t ... | 2006 | 16804442 |
| comparison of two commercial methods with pcr restriction fragment length polymorphism of the tuf gene in the identification of coagulase-negative staphylococci. | two commercial methods for the identification of coagulase-negative staphylococci (cns) were compared with the restriction fragment length polymorphism (rflp) of the amplified tuf gene, which served as the reference method. | 2006 | 16965378 |
| species distribution and properties of staphylococci from canine dermatitis. | the occurrence and phenotypic, and genotypic properties of 24 staphylococcus isolates from canine dermatitis were investigated. the predominant staphylococcal species was staphylococcus intermedius. the other species such as staphylococcus chromogenes, staphylococcus sciuri, staphylococcus aureus, staphylococcus saprophyticus, staphylococcus epidermidis, and staphylococcus capitis were only occasionally isolated. the study showed low level biochemical diversity among s. intermedius isolates. res ... | 2007 | 17126372 |
| antibiotic resistance patterns of coagulase-negative staphylococcus strains isolated from blood cultures of septicemic patients in turkey. | the aim of this study is to determine antibiotic resistance patterns and slime production characteristics of coagulase-negative staphylococci (cons) caused nosocomial bacteremia. a total of 200 cons strains were isolated from blood samples of patients with true bacteremia who were hospitalized in intensive care units and in other departments of istanbul university cerrahpasa medical hospital between 1999 and 2006. among 200 cons isolates, staphylococcus epidermidis was the most prevalent species ... | 2009 | 17475456 |
| staphylococcus pettenkoferi sp. nov., a novel coagulase-negative staphylococcal species isolated from human clinical specimens. | five coagulase-negative, novobiocin-susceptible staphylococcal strains were isolated from human blood cultures in different german and belgian medical facilities. a novel species, 'staphylococcus pettenkoferi' was proposed recently to accommodate two of these strains (b3117(t) and a6664), although the name was not validly published. all five strains belonged to the genus staphylococcus because they were non-motile, gram-positive, catalase-positive cocci with peptidoglycan type (a3 alpha type l-l ... | 2007 | 17625191 |
| almond oil implicated in a staphylococcus capitis outbreak in a neonatal intensive care unit. | to develop an effective outbreak-control strategy by identifying the source and modes of transmission of staphylococcus capitis in a 60-bed neonatal intensive care unit (nicu). | 2007 | 17805342 |
| molecular cloning and functional characterization of a histidine decarboxylase from staphylococcus capitis. | histamine intoxication is probably the best known toxicological problem of food-borne disease. a histamine-producing staphylococcus capitis strain has been isolated from a cured meat product. the aim of this study was to gain deeper insights into the genetic determinants for histamine production in staph. capitis. | 2008 | 17887985 |
| evolution of slime production by coagulase-negative staphylococci and enterotoxigenic characteristics of staphylococcus aureus strains isolated from various human clinical specimens. | the present study was designed to determine the slime production of coagulase-negative staphylococci (cons) and the enterotoxigenic properties of staphylococcus aureus strains, and to evaluate the clinical importance of slime-producing cons and enterotoxigenic s. aureus strains isolated from various human clinical specimens. for this purpose, a total of 120 staphylococcus strains were isolated and identified, and further characterized for their slime production and enterotoxigenicity. of the cli ... | 2007 | 17893164 |
| distribution of staphylococcal cassette chromosome mec (sccmec) types i, ii, iii and iv in coagulase-negative staphylococci from patients attending a tertiary hospital in southern brazil. | coagulase-negative staphylococci (cons) are now recognized as the aetiological agents of an important range of infections in humans. most developed countries have reported an increase in cons infections in hospitalized patients that are resistant to meticillin and other antibiotics. staphylococcal cassette chromosome mec (sccmec) typing is essential for understanding the molecular epidemiology of meticillin-resistant staphylococcus strains. sccmec elements are currently classified into types i t ... | 2007 | 17893169 |
| development of a real-time pcr assay for the detection and identification of staphylococcus capitis, staphylococcus haemolyticus and staphylococcus warneri. | staphylococcus capitis, staphylococcus haemolyticus and staphylococcus warneri are coagulase-negative staphylococci. each species has different characteristics, and a difference in pathology is also seen in compromised hosts. therefore, the development of a species-specific simple detection method for the identification of these staphylococci is important. here, a species-specific real-time pcr assay is reported that targets the superoxide dismutase a-encoding gene of these bacteria. primers wer ... | 2007 | 17893172 |
| occurrence of staphylococci in the oral cavities of healthy adults and nasal oral trafficking of the bacteria. | to investigate a possible peroral route of infective endocarditis (ie), the occurrence of staphylococci in the oral cavity was examined using saliva and supragingival plaque specimens from 56 systemically and periodontally healthy adults aged 22-43 years old (27.1+/-5.3). nine staphylococcus species and 334 isolates were identified. in saliva, the total occurrence rate was 83.9 % and the total number of bacteria was 10(2)-10(4) c.f.u. ml(-1). staphylococcus aureus was the most frequent species ( ... | 2008 | 18065673 |
| vancomycin heteroresistance in bloodstream isolates of staphylococcus capitis. | nine staphylococcus capitis isolates from blood cultures of newborns were examined for resistance to vancomycin. mics were within the susceptible range, but population profiling revealed a resistant subpopulation. only isolates with the largest subpopulation were identified as heteroresistant to vancomycin by etest. this finding may have therapeutic implications. | 2008 | 18596138 |
| staphylococcus capitis prosthetic valve endocarditis: report of two rare cases and review of literature. | staphylococcus epidermidis is the most common organism associated with prosthetic valve endocarditis. staphylococcus capitis, a coagulase-negative staphylococcus, is a rare cause of endocarditis. we report two cases of s.capitis prosthetic valve endocarditis, both involving prosthetic aortic valve and complicated by aortic root abscess. we also review the literature for this rare condition caused by this rare organism. | 2008 | 18790338 |
| characteristic cell wall ultrastructure of a macrolide-resistant staphylococcus capitis strain isolated from a patient with chronic sinusitis. | fourteen-membered-ring macrolides have an antiinflammatory effect, in addition to their antibacterial effect, and are widely used at low dosages for long-term therapy for chronic inflammatory disease such as diffuse pan-bronchiolitis and chronic sinusitis. a macrolide-resistant coagulase-negative staphylococcal strain was obtained from the maxillary sinus of a patient with chronic sinusitis, who failed long-term macrolide therapy. the isolated strain was characterized as staphylococcus capitis a ... | 2008 | 18807142 |
| prevalence and herd-level risk factors for intramammary infection with coagulase-negative staphylococci in dutch dairy herds. | in this study, the prevalence of intramammary infection (imi) with coagulase-negative staphylococci (cns) in the netherlands was estimated on 49 randomly selected herds with at least 40 lactating cows. in total, 4220 quarter milk samples were collected. the prevalence of cns imi in the netherlands was estimated at 10.8% at quarter level and 34.4% at cow level, making it the most frequently isolated group of pathogens. fourteen species of cns were identified; the most frequently isolated species ... | 2009 | 18977613 |
| primary chronic osteomyelitis of the jaw--a microbial investigation using cultivation and dna analysis: a pilot study. | the aims of this study were to investigate the infectious etiology of primary chronic osteomyelitis (pco) of the jaw using an extraoral sampling approach and molecular methods. | 2009 | 19426919 |
| screening of platelet concentrates for bacterial contamination: spectrum of bacteria detected, proportionof transfused units, and clinical follow-up. | screening of platelet concentrates (pcs) for bacterial contamination with cultivation methods is carried out as a routine procedure in some countries. the aim is to prevent the transfusion of contaminated pcs. the german evaluation of regular monitoring study group conducted a prospective multicenter study on 52,243 pcs to investigate the prevalence of bacteria (bact/alert, biomerieux). this study describes the detected bacterial spectrum, the proportion of pcs with a positive test result that h ... | 2009 | 19484239 |
| in vivo model of human pathogen infection and demonstration of efficacy by an antimicrobial pouch for pacing devices. | device-related infections represent a significant clinical challenge. once established, these infections prove difficult to treat with existing antibiotic regimens, compromising the health of device recipients, and usually requiring surgical intervention to resolve. | 2009 | 19572866 |
| rapid and sensitive microplate assay for screening the effect of silver and gold nanoparticles on bacteria. | nanomaterials are the leading requirement of the rapidly developing field of nanomedicine and bionanotechnology, and in this respect, nanotoxicology research is gaining great importance. in the field of infections, nanoparticles are being utilized as therapeutic tools against microbes, thus understanding the properties of nanoparticles and their effect on microbes is essential prior to clinical application. the aim of this study was to evaluate a microplate-based assay for monitoring the toxicit ... | 2009 | 19663592 |
| metabolism of azo dyes by human skin microbiota. | reduction of methyl red (mr) and orange ii (or ii) by 26 human skin bacterial species was monitored by a rapid spectrophotometric assay. the analysis indicated that skin bacteria, representing the genera staphylococcus, corynebacterium, micrococcus, dermacoccus and kocuria, were able to reduce mr by 74-100 % in 24 h, with only three species unable to reduce completely the dye in that time. among the species tested, only corynebacterium xerosis was unable to reduce or ii to any degree by 24 h, an ... | 2010 | 19729456 |
| [detection of meca gene in oxacillin-resistant coagulase-negative staphylococci isolated from the saliva of nursing professionals]. | coagulase-negative staphylococci are frequently associated with nosocomial infections, and healthcare professionals can be reservoirs and spread them in hospitals and in the community. the aim of this study was to identify species of coagulase-negative staphylococci isolated from the saliva of nursing professionals, determine the resistance profile and detect the meca gene. one hundred coagulase-negative staphylococci were selected: 41 were identified as staphylococcus epidermidis, 25 as staphyl ... | 2009 | 19802475 |
| isolation of cr(vi) reducing bacteria from industrial effluents and their potential use in bioremediation of chromium containing wastewater. | the present study was aimed to assess the ability of bacillus sp. jdm-2-1 and staphylococcus capitis to reduce hexavalent chromium into its trivalent form. bacillus sp. jdm-2-1 could tolerate cr(vi) (4800 microg/ml) and s. capitis could tolerate cr(vi) (2800 microg/ml). both organisms were able to resist cd2+ (50 microg/ml), cu2+ (200 microg/ml), pb2+ (800 microg/ml), hg2+ (50 microg/ml) and ni2+ (4000 microg/ml). s. capitis resisted zn2+ at 700 microg/ml while bacillus sp. jdm-2-1 only showed r ... | 2009 | 19803088 |
| antibacterial effect of some benzopyrone derivatives. | the schiff base derivatives of benzopyran-4-one are characterized by imine groups which have biological activities such as antimicrobial, antifungal and antitumoral. the aim of this study was therefore to evaluate the toxicity of schiff bases towards bacterial cells. escherichia coli as gram-negative bacteria and staphylococcus capitis as gram-positive bacteria were exposed to different concentrations of schiff bases. for assessment of toxicity a 96-well turbidimetric procedure, capable of testi ... | 2010 | 19896248 |
| unilateral scrotal pyocele in ram caused by staphylococcus capitis. | a massive unilateral scrotal pyocele caused by staphylococcus capitis in a 6-year-old ram is reported. ultrasound examination of the right hemiscrotum showed an irregular hyperechoic mass in an anechoic fluid. a dense exudate was collected from the scrotum for microbiological analysis. grossly, there was an extensive greenish purulent exudate and a completely atrophied right testis. coagulase-negative s. capitis was isolated in pure culture. to the authors' knowledge, this is the first report of ... | 2009 | 19930163 |
| quality monitoring of microbial contamination of cryopreserved parathyroid tissue. | cryopreservation of parathyroid tissue (pt) provides patients undergoing parathyroidectomy with an option for delayed autologous heterotopic parathyroid transplantation. a standard protocol for quality monitoring of pt has not been established. this article describes a method for detecting the presence of bacterial contamination in pt tissue intended for autologous transplantation. pt was received in the tissue bank, processed under aseptic conditions, and placed into cryopreservation medium. st ... | 2011 | 20058088 |
| evaluation of matrix-assisted laser desorption ionization-time-of-flight mass spectrometry in comparison to rpob gene sequencing for species identification of bloodstream infection staphylococcal isolates. | as a result of variable expression of biochemical characters, misidentification by conventional phenotypic means often occurs with clinical isolates belonging to staphylococcus species. therefore, we evaluated the use of matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (maldi-tof ms) for the identification of 450 blood isolates of the most relevant staphylococcal species, using sequence analysis of the rpob gene as the reference method. a correct species identificatio ... | 2011 | 20132252 |
| identification and characterization of methicillin-resistant coagulase-negative staphylococci from bovine mastitis. | this study focused on the correlation between geno- and phenotypic tests in the correct assessment of meca-mediated methicillin resistance among coagulase-negative staphylococci (cons) and the further characterization of meca-positive isolates. | 2010 | 20525989 |
| polymerase chain reaction detection of enterotoxins genes in coagulase-negative staphylococci isolated from brazilian minas cheese. | for a long time, staphylococcus aureus has been always thought to be the only pathogenic species among staphylococcus, while coagulase-negative staphylococci (cns) were classified as contaminant agents. however, molecular techniques have shown that these microorganisms also possess enterotoxin-encoding genes. the aim of this study was to analyze the frequency of genes for staphylococcal enterotoxins sea, seb, sec, and sed in cns strains isolated from minas soft cheese and to assess the in vitro ... | 2010 | 20528174 |
| mechanisms of oral bacteria-induced platelet activation. | the oral cavity is inhabited by over 500 different bacterial species that normally exist in ecological balance both with each other and with the host. when this equilibrium is disturbed, an overgrowth of individual organisms can occur, which, in turn, can lead to the onset of pathological processes, notably dental caries and periodontitis. generally, bacteraemias occur more frequently in individuals with periodontal disease, and these bacteraemias have been implicated in the development of a ran ... | 2010 | 20555421 |
| activity of telavancin and comparator antimicrobial agents tested against staphylococcus spp. isolated from hospitalised patients in europe (2007-2008). | the activity of telavancin was evaluated against staphylococcus spp. collected from european hospitals as part of an international surveillance study (2007-2008). a total of 7534 staphylococcal clinical isolates [5726 staphylococcus aureus and 1808 coagulase-negative staphylococci (cons)] were included. isolates were tested for susceptibility according to reference methods and minimum inhibitory concentration (mic) values were interpreted based on clinical and laboratory standards institute (cls ... | 2010 | 20598860 |