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staphylococcus cohnii subspecies: staphylococcus cohnii subsp. cohnii subsp. nov. and staphylococcus cohnii subsp. urealyticum subsp. nov.two major subspecies of staphylococcus cohnii, namely s. cohnii subsp. cohnii, from humans, and s. cohnii subsp. urealyticum, from humans and other primates, are described on the basis of a study of 14 to 25 strains and 18 to 33 strains, respectively. dna-dna hybridization studies conducted in our laboratory in 1983 (w. e. kloos and j. f. wolfshohl, curr. microbiol. 8:115-121, 1983) demonstrated that strains representing the different subspecies were significantly divergent. s. cohnii subsp. ure ...19911854641
a quantitative study of enterotoxin production by sheep milk staphylococci.of 124 staphylococcal strains isolated from sheep milk, 78 produced enterotoxin a, b, c, or d when evaluated by an enzyme-linked immunosorbent assay. enterotoxins a and d, elaborated by 44 and 43 strains, respectively, showed the highest incidence. enterotoxin production by coagulase-negative strains (one staphylococcus cohnii, three s. epidermidis, five s. haemolyticus, and four s. xylosus) was detected. linear and logarithmic-logarithmic regressions of optical density on enterotoxin concentrat ...19883355142
speciation and antibiotic susceptibility patterns of coagulase-negative staphylococci.during a six month period, 191 isolates of coagulase-negative staphylococci from blood, urine, cerebrospinal fluid and heart valves were identified to species level and tested for antimicrobial susceptibility. seventy-one percent of isolates were staphylococcus epidermidis, 8% staphylococcus warneri, 7% staphylococcus hominis, 7% staphylococcus haemolyticus, 4% staphylococcus capitis, 2% staphylococcus saprophyticus and 1% staphylococcus cohnii. approximately 4% of isolates were felt to be assoc ...19827173185
posttraumatic meningitis: bacteriology, hydrocephalus, and outcome.to investigate the conditions that have developed in the treatment of posttraumatic meningitis with the use of new antibiotics, the authors studied cases with this infection retrospectively for a period of 68 months. among 860 patients with moderate to severe head injuries, 12 (1.39%) sustained this complication. of these, nine patients (75%) had a demonstrable basilar skull fracture and seven (58.3%) presented obvious rhinorrhea. of these seven, four (57.1%) were treated conservatively and thre ...19947800133
staphylococcus cohnii: an unusual cause of primary septic arthritis in a patient with aids. 19968953078
chorioamnionitis as an apparent source of vertical transmission of staphylococcus cohnii and ureaplasma urealyticum to a neonate. 19989923526
epidemiological investigation of an animal house based upon phage-typing and biotyping of staphylococcus epidermidis.the value of biotyping and phage-typing coagulase-negative staphylococci in the epidemiological investigation of a laboratory animal house was clearly demonstrated. in the animal rooms in which conventional bacteriological methods revealed equal bacterial contamination between a conventional unit and one housing specified-pathogen-free rodents, biotyping identified staphylococcus cohnii as the only species in the latter, compared to s. warneri, s. hominis, s. saprophyticus. s. xylosus abd s. epi ...1979162239
species identification and phylogenetic relationships based on partial hsp60 gene sequences within the genus staphylococcus.the phylogenetic relationships among 36 validly described species or subspecies within the genus staphylococcus were investigated by cloning and sequencing their 60 kda heat-shock protein (hsp60) genes using a set of universal degenerate hsp60 pcr primers. the cloned partial hsp60 dna sequences from nine staphylococcus aureus strains were highly conserved (97-100% dna sequence similarity; mean 98%), indicating that the hsp60 gene of multiple isolates within the same species have little microhete ...199910425778
haemagglutination by staphylococcus saprophyticus and other staphylococcal species.staphylococcus saprophyticus was found to differ from staphylococcus epidermidis and staphylococcus aureus by its ability to agglutinate sheep erythrocytes. on testing 30 strains of each species, 28 strains of s. saprophyticus and one strain each of the other two species, caused agglutination. twenty-eight of 30 strains of staphylococcus cohnii and staphylococcus xylosis failed to cause haemagglutination. the haemagglutinating activity of s. saprophyticus, when using a 10 per cent bacterial susp ...1979107721
staphylococcus cohnii and ureaplasma urealyticum in a neonate. 199910482038
interaction of the staphylococcin-like peptide pep 5 with cell walls and isolated cell wall components of gram-positive bacteria.unlike bacteriocins of gram-negative bacteria, the strongly basic staphylococcin-like peptide pep 5 lacked specific receptor mediated binding to sensitive gram-positive bacteria. studies with whole cells, purified cell walls, teichoic acids, and lipoteichoic acids strongly suggested that it binds reversibly via electrostatic interaction to negatively charged groups. thus, pep 5 binding could be reversed by sufficiently high concentrations of monovalent (k+, 150-250 mm) and divalent (ca2+, 15-30 ...19854082823
mode of action of the peptide antibiotic nisin and influence on the membrane potential of whole cells and on cytoplasmic and artificial membrane vesicles.the peptide antibiotic nisin was shown to cause a rapid efflux of amino acids and rb+ from the cytoplasm of gram-positive bacteria (staphylococcus cohnii 22, bacillus subtilis w 23, micrococcus luteus atcc 4698, and streptococcus zymogenes 24). it strongly decreased the membrane potential of cells as judged by the distribution of the lipophilic tetraphenylphosphonium cation. ascorbate-phenazine methosulfate-driven transport of l-proline by cytoplasmic membrane vesicles was blocked after addition ...19854015074
induction of autolysis of staphylococci by the basic peptide antibiotics pep 5 and nisin and their influence on the activity of autolytic enzymes.pep 5 and nisin are cationic bactericidal peptides which were shown to induce autolysis in staphylococcus cohnii 22. in contrast to nisin, pep 5 induced lysis could be stimulated in the presence of glucose. addition of lipoteichoic acids (lta) (d-alanine:phosphorus = 0.475:1) inhibited all effects of pep 5 on susceptible cells in a molar ratio lta:pep 5 of 10:1. treatment of s. cohnii 22 with pep 5 or nisin for 20 min and subsequent washing with 2.5 m nacl released autolysin activity. crude prep ...19854004448
voltage-dependent depolarization of bacterial membranes and artificial lipid bilayers by the peptide antibiotic nisin.the peptide antibiotic nisin is shown to disrupt valinomycin-induced potassium diffusion potentials imposed on intact cells of staphylococcus cohnii 22. membrane depolarization occurred rapidly at high diffusion potentials while at low potentials nisin-induced depolarization was slower suggesting that nisin requires a membrane potential for activity. this assumption was proven in experiments with planar lipid bilayers (black lipid membranes). macroscopic conductivity measurements indicated a vol ...19873442448
mode of action of the staphylococcinlike peptide pep 5: voltage-dependent depolarization of bacterial and artificial membranes.the cationic staphylococcinlike peptide pep 5 is shown to depolarize bacterial and planar lipid membranes in a voltage-dependent manner. an artificial valinomycin-induced potassium diffusion potential across the cytoplasmic membrane of staphylococcus cohnii 22 was sufficient to promote pep 5 action. thus, evidence is provided that a membrane potential of sufficient magnitude is the only prerequisite for pep 5 activity. the voltage dependence was elucidated by macroscopic conductance measurements ...19883335484
fatal meningitis due to staphylococcus cohnii. case report.we report a case of fatal meningitis due to staphylococcus (s.) cohnii in a 63-year-old male. s. cohnii is often isolated from farm animals and known to be less pathogenic in humans. to our knowledge, s. cohnii has not yet been reported to cause infection of the central nervous system in humans.19911944939
molecular techniques open up new vistas for typing of coagulase-negative staphylococci.several methods were used to type 64 clinical isolates of coagulase-negative staphylococci (cns) derived from hospitals in morocco. the clinical isolates originated principally from blood cultures and wound sources. these isolates provided the opportunity to substantially compare the proficiency of developing molecular techniques with conventional phenotypic tests for use in the identification of clinical staphylococci. the following molecular methods were examined: utility ribotyping analysis ( ...200111367554
bacterial flora of the conjunctiva and nasal cavity in normal and diseased captive bustards.a survey was carried out to describe the normal aerobic bacterial flora of the conjunctiva and nasal cavity of captive houbara bustards (chlamydotis undulata), kori bustards (ardeotis kori), and white-bellied bustards (eupodotis senegalensis) maintained at the national avian research center, abu dhabi, united arab emirates. a total of 58 samples were examined from the nasal cavity and 55 samples from the conjunctiva of healthy bustards. there was no bacterial growth in 45% of conjunctival sample ...200111417826
susceptibility of staphylococcus species and subspecies to teicoplanin.twenty-four staphylococcus species and their subspecies were examined for their susceptibilities to teicoplanin by disk diffusion (30-micrograms disk) and agar dilution for the determination of mics. moderately susceptible and resistant clinical strains were further tested for their susceptibilities to oxacillin and vancomycin. teicoplanin resistance was not observed in the reference strains of the various staphylococcus species isolated from healthy volunteers or animals. however, the novobioci ...19911835340
susceptibility of staphylococcus species and subspecies to fleroxacin.twenty-four staphylococcus species or subspecies were examined for their susceptibilities to the fluoroquinolone fleroxacin (ro 23-6240) by disk diffusion (5-micrograms disk) and by agar dilution for the determination of mics. resistant strains were further tested for their susceptibilities to oxacillin and the fluoroquinolone ciprofloxacin. reference strains of the novobiocin-resistant species (staphylococcus saprophyticus, staphylococcus cohnii, staphylococcus xylosus, staphylococcus arlettae, ...19911759838
risk of bacteremia induced by transesophageal echocardiography: analysis of 100 consecutive procedures.the incidence of bacteremia induced by transesophageal echocardiography (tee) and, consequently, the need for an antibiotic prophylaxis before tee is still controversial. therefore, we studied the incidence of bacteremia associated with tee prospectively in 100 consecutive patients without clinical or laboratory signs of bacterial infection. blood samples were drawn immediately before and at 0, 5, and 15 minutes after tee. in addition, swabs were taken from the pharyngeal region before tee and f ...19921571172
[the effect of culture media on bacteriocin production in various strains of bacteria].some staphylococcus and enterococcus strains were used to investigate the effect of culture medium on bacteriocin production. staphylococcus cohnii sc7, staphylococcus sp. ztj 151, s. saprophyticus ss 877, enterococcus faecium ef1 and e. faecalis efg2 were isolated from the rumen wall and contents of lambs, calves and fallow deer, enterococcus gallinarum eg10 and e. avium ea12 were isolated from the caecum of japanese quail. the tested bacteria belong to producers with a wide antimicrobial effec ...19921297243
staphylococcus cohnii: a case report on an unusual pathogen.coagulase-negative staphylococci have become increasingly important causes of infection in predisposed hosts. such as patients receiving immunosuppressive therapy and broad-spectrum antimicrobial drugs, patients who have prosthetic devices, or those who have prolonged hospital or intensive care unit stays. however, human infections caused by staphylococcus cohnii rarely have been reported in the literature. in this report, we review the current literature and describe a 38 year-old immunosuppres ...199610172625
characterization of a staphylococcal plasmid related to pub110 and carrying two novel genes, vatc and vgbb, encoding resistance to streptogramins a and b and similar antibiotics.we isolated and sequenced a plasmid, named pip1714 (4,978 bp), which specifies resistance to streptogramins a and b and the mixture of these compounds. pip1714 was isolated from a staphylococcus cohnii subsp. cohnii strain found in the environment of a hospital where pristinamycin was extensively used. resistance to both compounds and related antibiotics is encoded by two novel, probably cotranscribed genes, (i) vatc, encoding a 212-amino-acid (aa) acetyltransferase that inactivates streptogrami ...19989661023
staphylococcus aureus in dairy products in the bologna area.a study was carried out to evaluate the extent of contamination by staphylococcus spp. and in particular by staphylococcus aureus in dairy products on sale in the bologna area. staphylococcus aureus was found in 16.30% of the 135 cheese samples examined: 8.3% in soft cheese, 17.60% in blue cheese, 18.9% in semi-soft cheese and 25.0% in mozzarella-type cheese. the mean concentration was 2699 cfu/g in the 22 positive samples. the most commonly found species were staphylococcus epidermidis (14.8%), ...19979105936
gram-positive, catalase-positive cocci from dry cured iberian ham and their enterotoxigenic potential.iberian ham is an uncooked, cured meat product ripened under natural uncontrolled conditions for 18 to 24 months. gram-positive, catalase-positive cocci are the main microbial population in iberian ham for most of the ripening time. since some of these organisms are able to produce enterotoxins, adequate characterization and toxicological study are needed. for this, 1,327 gram-positive, catalase-positive cocci, isolated from iberian hams at different stages and locations, were characterized by p ...19968787389
coagulase-negative staphylococci in danish blood cultures: species distribution and antibiotic susceptibility.the distribution and antibiotic susceptibility of coagulase-negative staphylococci (cons) isolated from blood cultures was examined in samples from hospitals covering most of denmark. a total of 499 cons isolates were detected in 477 blood cultures from 340 patients and speciated as staphylococcus epidermidis, 285; staphylococcus hominis, 61; staphylococcus haemolyticus, 43; staphylococcus warneri, 12; staphylococcus cohnii, 7; staphylococcus saprophyticus, 4; staphylococcus capitis, 2 and staph ...19968690885
neonatal sepsis and meningitis due to staphylococcus cohnii. 19968683849
community-acquired pneumonia due to staphylococcus cohnii in an hiv-infected patient: case report and review.coagulase-negative staphylococci recently have been implicated as a cause of serious infections in immunocompromised individuals. an unusual case of community-acquired pneumonia due to staphylococcus cohnii in an hiv-infected drug user is described. results of a study conducted to examine the prevalence of infection due to staphylococcus cohnii strains and their antibiotic-sensitivity patterns show a low frequency but a high morbidity. these results and a brief review of the literature emphasize ...19958605905
widespread distribution of disinfectant resistance genes among staphylococci of bovine and caprine origin in norway.we demonstrate here a widespread distribution of genes mediating efflux-based resistance to quaternary ammonium compounds (qacs) in staphylococci from unpasteurized milk from 127 dairy cattle herds and 70 dairy goat herds. qac resistance genes were identified in 21% of the cattle herds (qaca/b, smr, qacg, and qacj) and in 10% of the goat herds (qaca/b and smr). further examination of 42 qac-resistant bovine and caprine isolates revealed the following genes: qaca/b (12 isolates) was present in fo ...200516145078
[resistance to heavy metals in ruminal staphylococci].ruminal, coagulase-negative, urease and bacteriocin-like substances producing staphylococci were screened for their heavy metal ions and antibiotics resistance. all strains tested were resistant to disodium arsenate at a minimal inhibition concentration (mic > 5 g/l) and cadmium sulphate (mic > 4 g/l). mic = 50-60 mg/l was determined in eight staphylococci screened in mercury chloride resistance test (tab. i). silver nitrate resistance was detected in seven of the bacteria used (mic = 40-50 mg/l ...19948073587
gas-liquid chromatography of cellular fatty acids for identification of staphylococci.a commercially available, computer-assisted microbial identification system (mis) employs gas-liquid chromatographic analyses of bacterial fatty acids. the mis was used to identify 470 isolates of staphylococcus species. the accuracy of the mis was compared with the accuracies of conventional methods. there was a complete agreement between the mis and conventional methods in the identification of 413 (87.8%) strains. for 36 of 45 misidentified strains, the correct identification was listed by th ...19947989541
four-year prospective study of staph-ident system and conventional method for reference identification of staphylococcus, stomatococcus, and micrococcus spp..a 4-year prospective study compared the accuracy of the staph-ident system (biomérieux vitek, inc., hazelwood, mo.) with that of the reference procedure of the centers for disease control and prevention for the identification of staphylococcus species, stomatococcus mucilaginosus, and micrococcus species. the study compared the results from 1,106 cultures (500 eye cultures, 217 strains submitted for reference identification, and 389 known stock strains) representing 21 species of the family micr ...19957699074
iron supply of staphylococci and of micrococci by alpha-ketoacids.most of 232 strains of coagulase-negative staphylococci (cns) belonging to 16 species, and nine strains of micrococci belonging to four species, could be distinguished by their growth on an iron-poor nutrient medium. alpha-keto-/alpha-hydroxyacids, as primary metabolites, facilitated iron supply to cns and micrococci under various iron-limited conditions, as detected by different bioassay methods. twelve selected cns strains and two micrococcal strains produced significant amounts of alpha-ketoa ...19957608951
the vitamin requirements of staphylococcus cohnii.the vitamin requirements of 66 strains of staphylococcus cohnii from various sources were determined using solidified and liquid chemically defined media. all the strains tested required nicotinic acid and thiamine. biotin was either growth stimulatory or essential for 34 strains. pantothenic acid was required by 21 of the 30 strains of known human origin but was not required by any of the 24 strains of murine origin. there was an association among staph. cohnii strains to grow in the absence of ...19836853396
mannose inhibition as a significant marker for differentiating among novobiocin-resistant staphylococci of relevance in clinical microbiology.a specific method for the identification of staphylococcus saprophyticus among novobiocin-resistant species isolated from man is described. the test is based on novobiocin resistance, non-fermentation of d-mannose and early inhibition with late secondary growth on glucose/mannose + novobiocin agar plates. on this medium novobiocin-resistant staphylococcus cohnii showed a confluent, continuous and homogeneous growth after 24 h which remained unchanged at 48 h whether or not it fermented d-mannose ...19873668496
production of volatile aroma compounds by bacterial strains isolated from different surface-ripened french cheeses.twelve bacterial strains belonging to eight taxonomic groups: brevibacterium linens, microbacterium foliorum, arthrobacter arilaitensis, staphylococcus cohnii, staphylococcus equorum, brachybacterium sp., proteus vulgaris and psychrobacter sp., isolated from different surface-ripened french cheeses, were investigated for their abilities to generate volatile aroma compounds. out of 104 volatile compounds, 54 volatile compounds (identified using dynamic headspace technique coupled with gas chromat ...200717701035
prolonged inflammatory syndrome revealing asymptomatic staphylococcus cohnii infection of spinal fixation material. 200817913556
biochemical and molecular characterization of erthromycin-resistant avian staphylococcus spp. isolated from chickens.the epidemiology of the two common erythromycin-resistant methylase (erm) genes ermc and erma was analyzed in 12 coagulase-negative staphylococcus spp. and 34 coagulase-positive staphylococcus spp. isolated from chicken. southern hybridization indicated that only 2 of the 12 coagulase-negative staphylococcus spp. strains contained the ermc gene on the plasmid; 1 strain of staphylococcus xylosus harbored the ermc gene on a 2.5-kb plasmid, and 1 strain of staphylococcus cohnii harbored the gene on ...199910472846
beta-hemolytic streptococci and other beta-hemolytic organisms in apical periodontitis and severe marginal periodontitis.thirteen teeth with necrotic pulps and apical periodontitis and nine severe periodontal pockets were cultured for presence of beta-hemolytic streptococci and other beta-hemolytic organisms. samples were dispersed and plated on two non-selective and one selective growth media and incubated anaerobically and in 10% co2 in air. a total of 59 beta-hemolytic colonies were purified and identified. eight beta-hemolytic streptococcal isolates were obtained from three of the severe marginal periodontitis ...199910530151
[influence of staphylococcin t on enterococcus sp. growth].bacteriocins are ribosomally synthesised, extracellular bacterial products. generally, spectrum of inhibition is limited to the same or closely related species to bacteriocin producer. staphylococcin t is produced by staphylococcus cohnii strain. the present study concerns influence of stt to 267 enterococcus sp. strains growth isolated between 2003 and 2006 in department of microbiology university hospital of dr. a. jurasz in bydgoszcz. s. cohnii t antagonistic ability evaluated towards bacteri ...200718416123
synthesis of siderophores by strains of staphylococcus cohnii isolated from various environments.siderophore activity as the feature of microorganisms enabling colonization of human body and the survival in inanimate environment was investigated in 108 strains of staphylococcus cohnii; s. cohnii ssp. cohnii (50 strains) and s. cohnii ssp. urealyticus (58 strains). strains were isolated from people, hospital and non-hospital environment. highest siderophore activity was noted in strains s. cohnii ssp. urealyticus particularly from the inanimate environments origin. in 86% analyzed strains si ...200314743978
development of a large-scale hplc-based purification for the urease from staphylococcus leei and determination of subunit structure.coagulase-positive staphylococcus species, related to but distinct from the genetic homology group containing staphylococcus cohnii, staphylococcus xylosus, and staphylococcus saphrophyticus, were isolated from biopsy material obtained from a cluster of patients in korea suffering from gastritis. the prototype isolate, staphylococcus leei, has high urease activity that is similar with respect to a low k(m) value and acid resistance of the urease found in the stomach adapted pathogen, helicobacte ...200414766306
identification, molecular biotyping and ultrastructural studies of bacterial communities isolated from two damaged frescoes of st damian's monastery in assisi.to investigate the composition of the microbial community in biodeterioration of two frescoes in st damian's monastery in assisi.200415130137
linezolid-resistant staphylococcus cohnii, greece. 200919116069
plasmids of staphylococcus cohnii isolated from the intensive-care unit.numerous isolates of both subspecies of staphylococcus cohnii were found in the environment of the intensive-care unit of a pediatric hospital. these isolates carried in their cells many plasmids, up to fourteen, of a wide range of sizes (< 2 to > 56 kb). striking was the occurrence of large plasmids not very common in staphylococci. these were present in > 80% of s. cohnii isolates. fifty-two different plasmid profiles were found in 79 investigated isolates belonging to s. cohnii ssp. cohnii an ...200415227782
in vitro activity of 24 antimicrobial agents against staphylococcus and streptococcus isolated from diseased animals in japan.a total of 88 staphylococcus and 61 streptococcus isolates from diseased animals throughout japan were examined in 2000 for the minimum inhibitory concentrations of 24 different antimicrobials by the agar dilution method standardized by the japanese society of chemotherapy. the resistance rates to aminobenzylpenicillin (36.4%) and benzylpenicillin (35.2%) were high in staphylococcus isolates, and those to oxytetracycline (45.9%) and kanamycin (21.3%) were high in streptococcus isolates. two isol ...200515750321
identification, characterization and purification of the lantibiotic staphylococcin t, a natural gallidermin variant.staphylococcin t (stt), an antibacterial agent produced by a staphylococcus cohnii t strain, was purified to homogeneity by ammonium sulphate precipitation, gel filtration, cation exchange and fast performance liquid chromatography (fplc). the final yield was about 20%, and over a 1000-fold increase in the specific activity was obtained. mass determination (2166 da), amino acid sequencing (ile-ala-xaa-lys-phe-leu-xaa-xaa-pro-gly-xaa-ala-lys-block) and dna sequencing demonstrated that stt is iden ...199910664909
antimicrobial susceptibility and presence of resistance genes in staphylococci from poultry.the species distribution, susceptibility to 19 antimicrobial agents and presence of selected genes encoding resistance to macrolides, streptogramins and tetracyclines were examined among 118 staphylococcal isolates from infections of poultry in denmark. isolates were identified using a combination of conventional biochemical testing and 16s rdna sequencing. the most common species were staphylococcus aureus (83), staphylococcus hyicus (11), staphylococcus xylosus (9) and staphylococcus cohnii (6 ...200010831857
predominant staphylococci in the intensive care unit of a paediatric hospital.coagulase-negative staphylococci cause a significant number of infections, especially in immunocompromised patients, including premature neonates. nosocomial strains present in the environment create a special risk.we studied staphylococci isolated from the intensive care unit of a paediatric teaching hospital over the period of six months in 1997. biotyping and species identification were performed; resistance to methicillin and other beta-lactam antibiotics and patterns of resistance to antimi ...200010860691
antimicrobial activity of bark extracts of syzygium jambos (l.) alston (myrtaceae).syzygium jambos (l.) alston (myrtaceae) is a widespread medicinal plant traditionally used in sub-saharan africa to treat infectious diseases. acetone and aqueous extracts from the bark of s. jambos were tested for antimicrobial activity in vitro by the agar dilution method in petri dishes. both extracts showed some activity against the tested micro-organisms. they proved to be particularly effective on staphylococcus aureus, yersinia enterocolitica and coagulase negative staphylococci among whi ...200010904178
staphylococcus cohnii--resident of hospital environment: cell-surface features and resistance to antibiotics.staphylococcus cohnii strains dominated in the environment of investigated hospitals. we isolated 420 strains of the species mainly from hospitals environments, but also from infants--intensive care units patients, its medical staff and non-hospital environments. s. cohnii subspecies cohnii was seen to dominate (361 strains). seventy seven percent of these strains expressed cell-surface hydrofobicity, most of them were slime producers (61%) and this feature was correlated with their methicillin ...200011093675
diversity of staphylococcal cassette chromosome mec structures in coagulase-negative staphylococci and relationship to drug resistance.the objective of this study was to determine the distribution of staphylococcal cassette chromosome mec (sccmec) elements in meticillin-resistant coagulase-negative staphylococci (mr-cons) isolated from a tertiary-care hospital in mexico and to examine the relationship to drug resistance. fifty selected mr-cons isolates collected from catheters (n=15), blood (n=15), bone (n=9), bronchial lavage (n=2) and urine (n=2) and one isolate each from an abscess, cerebrospinal fluid, eye, pleural effusion ...201020007762
[ability of staphylococcus cohnii strains to adhere to epithelial cells and solid surfaces in the hospital environment].presented study describes abilities of staphylococci to adhere to exfoliated cheek and uroepithelial epithelium cells and to various surfaces such as plastics, glass and steel. the subject of the study were strains of staphylococcus cohnii ssp. cohnii and staphylococcus cohnii ssp. urealyticus isolated from intensive care unit of pediatric hospital. staphylococcus cohnii ssp.cohnii adhered in great number to epithelial cells. however, the adhesion differed by individual strains. we did not find ...200212185691
staphylococcus cohnii septicaemia in a patient with colon cancer.a coagulase-negative staphylococcal strain was isolated from peripheral blood and central venous catheter blood of a febrile patient with cancer. this isolate, initially classified by a commercial test as staphylococcus kloosii, was definitively assigned to staphylococcus cohnii by physiological and molecular tests. the strain lacked virulence factors, such as biofilm production and haemagglutination, and was sensitive to the antibiotics tested. the data suggest that rare micro-organisms with lo ...200312488572
evaluation of selected features of staphylococcus cohnii enabling colonization of humans.based on iron utilization, sensitivity to skin fatty acids, lipolytic and proteolytic activity the potential abilities of staphylococcus cohnii strains to colonize humans were evaluated. the investigation included 60 strains that belong to both subspecies, viz. s. cohnii ssp. cohnii and s. cohnii ssp. urealyticus. strains were isolated from different sources of the intensive care unit and from non-hospital environment. most of the strains were multiple antibiotic-resistant. strains of both subsp ...200212503405
molecular diagnosis of infective endocarditis by pcr amplification and direct sequencing of dna from valve tissue.we used broad-range eubacterial pcr amplification followed by direct sequencing to identify microbial pathogens in heart valve material from 29 patients with histologically confirmed infective endocarditis and 23 patients free of infective endocarditis. microorganisms cultured by conventional techniques matched those identified by pcr in 21 cases. pcr alone identified the causative agent in three cases (streptococcus bovis, staphylococcus cohnii, and coxiella burnetii), allowing better patient m ...200312574279
comparative bioremediation potential of four rhizospheric microbial species against lindane.four microbial species (kocuria rhizophila, microbacterium resistens, staphylococcus equorum and staphylococcus cohnii subspecies urealyticus) were isolated from the rhizospheric zone of selected plants growing in a lindane contaminated environment and acclimatized in lindane spiked media (5-100 μg ml⁻¹). the isolated species were inoculated with soil containing 5, 50 and 100 mg kg⁻¹ of lindane and incubated at room temperature. soil samples were collected periodically to evaluate the microbial ...201021044795
staphylococcus nepalensis sp. nov., isolated from goats of the himalayan region.four coagulase-negative, novobiocin-resistant cocci, designated cw1(t), pm34, mm3 and rw78, were isolated from the respiratory tract of goats kept in the himalayan region. the four isolates were assigned to a single species on the basis of almost identical biochemical and physiological traits, protein profiles obtained after sds-page and identical genomic fingerprints generated after enterobacterial repetitive intergenic consensus (eric)-pcr. strain cw1(t) showed highest 16s rdna sequence simila ...200314657137
the amino acid sequences and activities of synergistic hemolysins from staphylococcus cohnii.staphylococcus cohnii ssp. cohnii and s. cohnii ssp. urealyticus are a coagulase-negative staphylococci considered for a long time as unable to cause infections. this situation changed recently and pathogenic strains of these bacteria were isolated from hospital environments, patients and medical staff. most of the isolated strains were resistant to many antibiotics. the present work describes isolation and characterization of several synergistic peptide hemolysins produced by these bacteria and ...200818752624
staphylococcus cohnii hemolysins - isolation, purification and properties.a total 355 of staphylococcus cohnii isolates from hospital environment, patients (newborns), medical staff and from non-hospital environment were tested for hemolytic activity. ninety-one % of s. cohnii ssp. cohnii and 74.5 % s. cohnii ssp. urealyticus strains exhibited hemolysis synergistic to s. aureus atcc 25923 strain. crude preparations of hemolysins of both bacterial subspecies presented delta-hemolysin, but not alpha- and beta-toxin activity. highly pure hemolysins were obtained by semip ...200919381478
sepsis due to linezolid resistant staphylococcus cohnii and staphylococcus kloosii: first reports of linezolid resistance in coagulase negative staphylococci from india.linezolid, a viable alternative to vancomycin against methicillin resistant staphylococcal isolates, has been in use for a decade around the globe. however, resistance against staphylococci remains extremely rare and unreported from most of the asian countries. herein, we report two cases of linezolid resistant, coagulase negative staphylococcal sepsis for the first time from india. the first case was an 18-year-old burn patient, who, after a major graft surgery, landed in sepsis, and linezolid ...201121304198
staphylococcus cohnii as a cause of multiple brain abscesses in weber-christian disease.we report a patient with multiple brain abscesses due to staphylococcus cohnii. while these brain abscesses markedly responded to the antibiotics, this patient was subsequently suffered from subcutaneous inflammatory nodules in the adipose tissue, which diagnosed him as having weber-christian disease (wcd). this is the first report that subcutaneous inflammatory nodules in the adipose tissue, which lead the diagnosis of wcd, followed multiple brain abscesses. to our knowledge, s. cohnii has not ...200516054652
[bacteriemia caused by staphylococcus cohnii associated with acute cholecystitis]. 200616596741
bacterial decontamination of duwl biofilm using oxygenal 6.the aim of this study was bacteriological assessment of the dental unit waterlines (duwl) biofilm - concentration and composition of the aerobe and facultative anaerobe bacterial microflora, and evaluation of the influence of a disinfecting product, oxygenal 6, on the biofilm composition. tubing fragments were taken from 25 units twice, before and after disinfection, and bacterial suspension of the biofilm was obtained from the samples. the bacterial flora was determined with the plate culture m ...200616841887
not so pretty in pink: staphylococcus cohnii masquerading as methicillin-resistant staphylococcus aureus on chromogenic media. 200617035496
[influence of testing conditions on the susceptibility results of staphylococcus cohnii to beta-lactams].the high occurence of coagulase-negative staphylococci among bacteria responsible for hospital infections is unquestioned. studies on the poorly-known novobiocin-resistant, coagulase-negative staphylococcus cohnii were undertaken. the possibilities of optimizing conditions for determination of susceptibility to beta-lactam antibiotics of this species were researched. in the case of s. cohnii the new cefoxitin test for detection of methicillin resistant strains, introduced by the national referen ...200617133902
antibiotic resistance patterns of coagulase-negative staphylococcus strains isolated from blood cultures of septicemic patients in turkey.the aim of this study is to determine antibiotic resistance patterns and slime production characteristics of coagulase-negative staphylococci (cons) caused nosocomial bacteremia. a total of 200 cons strains were isolated from blood samples of patients with true bacteremia who were hospitalized in intensive care units and in other departments of istanbul university cerrahpasa medical hospital between 1999 and 2006. among 200 cons isolates, staphylococcus epidermidis was the most prevalent species ...200917475456
bacterial contamination of water in dental unit reservoirs.the aim of this study was bacteriological assessment of water in dental unit reservoirs--concentration and composition of the aerobe and facultative anaerobe bacterial microflora. reservoir water samples were taken from 25 units. bacterial flora were determined with the plate culture method. bacteria were identified with biochemical microtests: api 20e, api 20ne (biomérieux, france) and gp2 microplatetm (biolog, usa). the concentration of total bacteria isolated from one site was 201,039 cfu/ml, ...200717655191
convective assembly of bacteria for surface-enhanced raman scattering.a sample preparation method based on convective assembly for "whole-microorganism" identification using surface-enhanced raman scattering (sers) is developed. with this technique, a uniform sample can easily be prepared with silver nanoparticles. during the deposition process, bacteria and nanoparticles are assembled to form a unique well-ordered structure with great reproducibility. the sers spectra acquired from the samples prepared with this technique have better quality and improved reproduc ...200818179261
crystal structure and mechanism of the staphylococcus cohnii virginiamycin b lyase (vgb).the semisynthetic streptogramin antibiotic quinupristin/dalfopristin (trade name synercid, aventis pharma) is a mixture of the a-type streptogramin dalfopristin and the b-type streptogramin quinupristin, a capped hexapeptide macrolactone. quinupristin/dalfopristin was developed to combat multidrug resistant pathogens, but suffers from its own problems with drug resistance. virginiamycin b lyase (vgb) inactivates the quinupristin component of synercid by lactone ring opening. remarkably, the enzy ...200818341294
[staphylococcus cohnii spp urealyticus: case report on an uncommon pathogen].coagulase-negative staphylococcus has emerged as an important agent in nosocomial infections. in this study, we report a case of bacteremia associated with a central venous catheter, caused by staphylococcus cohnii spp urealyticus that was isolated in blood cultures from a 53-year-old male patient who was admitted to a general hospital in the city of são paulo. we discuss in this report the difficulty in routinely identifying this microorganism in the clinical microbiology laboratory. staphyloco ...200818545844
evaluation of discrepancies between oxacillin and cefoxitin susceptibility in coagulase-negative staphylococci.the clinical and laboratory standards institute (clsi) recommends testing coagulase-negative staphylococci (cons) strains to determine resistance against oxacillin by testing for meca, pbp2a, or with cefoxitin disk. however, discrepant results of resistance to oxacillin and susceptibility to cefoxitin were found. in this study, we aimed to investigate the oxacillin resistance and cefoxitin susceptibility of cons in taiwan. of 9,017 strains collected from 2005 to 2010, 131 (1.5%) of the isolates ...201121249410
diversity of staphylococcal cassette chromosome mec structures in methicillin-resistant staphylococcus epidermidis and staphylococcus haemolyticus strains among outpatients from four countries.in staphylococci, methicillin (meticillin) resistance (mr) is mediated by the acquisition of the meca gene, which is carried on the size and composition variable staphylococcal cassette chromosome mec (sccmec). mr has been extensively studied in staphylococcus aureus, but little is known about mr coagulase-negative staphylococci (mr-cons). here, we describe the diversity of sccmec structures in mr-cons from outpatients living in countries with contrasting environments: algeria, mali, moldova, an ...200919001111
comparison of three phenotypic techniques for detection of methicillin resistance in staphylococcus spp. reveals a species-dependent performance.to evaluate the usefulness of the cefoxitin screen in vitek 2 gram-positive panels for recognizing methicillin-resistant strains of staphylococci.200919136679
the bacterial diversity of pharmaceutical clean rooms analyzed by the fatty acid methyl ester technique.clean rooms are largely employed in pharmaceutical companies whose purpose is to produce sterile drugs and devices. we employed gram staining and gas chromatography of fatty acid methyl esters extracted from whole cells of environmental isolates to characterize and identify bacteria isolated in each of 50 different clean rooms designed for the manufacturing of sterile medicinal products and to provide a fatty acid profile of the most common species of isolated bacteria. statistical analysis allo ...201021502015
the uropathogenic species staphylococcus saprophyticus tolerates a high concentration of d-serine.human urine contains a relatively high concentration of d-serine, which is toxic to several nonuropathogenic bacteria, but can be utilized or detoxified by uropathogenic escherichia coli (upec). the sequenced genome of uropathogenic staphylococcus saprophyticus contains a gene with homology to the d-serine deaminase gene (dsda) of upec. we found the gene in several clinical isolates of s. saprophyticus; however, the gene was absent in staphylococcus xylosus and staphylococcus cohnii, phylogeneti ...200919674114
microbiological investigation of an antibacterial sandwich layer system.to allow medical application of an artificial bladder made of biocompatible polyurethane, a long-term stable antibacterial coating is required. alone, the artificial bladder exhibits no defense against microorganisms. silver coating provides long-term antibacterial protection by the continuous release of silver ions into aqueous solutions. to control and to prolong the rate of silver ion release, the deposited silver film has to be protected by an inert film of biocompatible polyparylene by mean ...201425280843
some coagulase-negative staphylococcus species affect udder health more than others.a longitudinal study in 3 dairy herds was conducted to profile the distribution of coagulase-negative staphylococcus (cns) species causing bovine intramammary infection (imi) using molecular identification and to gain more insight in the pathogenic potential of cns as a group and of the most prevalent species causing imi. monthly milk samples from 25 cows in each herd as well as samples from clinical mastitis were collected over a 13-mo period. coagulase-negative staphylococci were identified to ...201121524522
identification of a haemolysin-like peptide with antibacterial activity using the draft genome sequence of staphylococcus epidermidis strain a487.our interest in staphylococcus epidermidis strain a487 was prompted by the unusual nature of its inhibitory activity in screening tests against methicillin-resistant staphylococcus aureus isolates. the inhibitory activity was detected in deferred antagonism tests only if the agar plate was preheated for at least 35 min at ≥55 °c before inoculation of the indicator bacteria, this phenomenon indicating possible involvement of a heat-labile immunity agent or protease. the inhibitor was purified to ...201121539624
role of lipid ii and membrane thickness in the mechanism of action of the lantibiotic bovicin hc5.lantibiotics are antimicrobial peptides produced by gram-positive bacteria, nisin being the most well-known member. nisin inhibits peptidoglycan synthesis and forms pores at sensitive membranes upon interaction with lipid ii, the essential bacterial cell wall precursor. bovicin hc5, a bacteriocin produced by streptococcus bovis hc5, has the putative n-terminal lipid ii binding motif, and we investigated the mode of action of bovicin hc5 using both living bacteria and model membranes, with specia ...201121876041
distribution of the multidrug resistance gene cfr in staphylococcus spp. isolates from swine farms in china.a total of 149 porcine staphylococcus isolates with florfenicol mics of ≥16 μg/ml were screened for the presence of the multiresistance gene cfr, its location on plasmids and its genetic environment. in total, 125 isolates carried either cfr (16 isolates), fexa (92 isolates) or both genes (17 isolates). the 33 cfr-carrying staphylococci, which included isolates of the species s. cohnii, s. arlettae, and s. saprophyticus in which the cfr gene has not been described before, exhibited a wide variet ...201122183168
characterization of staphylococci in urban wastewater treatment plants in spain, with detection of methicillin resistant staphylococcus aureus st398.the objective of this study was to determine the prevalence of staphylococcus in urban wastewater treatment plants (uwtp) of la rioja (spain), and to characterize de obtained isolates. 16 wastewater samples (8 influent, 8 effluent) of six uwtps were seeded on mannitol-salt-agar and oxacillin-resistance-screening-agar-base for staphylococci and methicillin-resistant staphylococcus aureus recovery. antimicrobial susceptibility profile was determined for 16 antibiotics and the presence of 35 antimi ...201626840519
metabolism of azo dyes by human skin microbiota.reduction of methyl red (mr) and orange ii (or ii) by 26 human skin bacterial species was monitored by a rapid spectrophotometric assay. the analysis indicated that skin bacteria, representing the genera staphylococcus, corynebacterium, micrococcus, dermacoccus and kocuria, were able to reduce mr by 74-100 % in 24 h, with only three species unable to reduce completely the dye in that time. among the species tested, only corynebacterium xerosis was unable to reduce or ii to any degree by 24 h, an ...201019729456
[influence of ciprofloxacin on the ability of production of staphylococcin t in staphylococcus cohnii (stt)].the aim of this study was to evaluate the influence of ciprofloxacin in a concentration of 0.25 microg/ml on the ability of synthesis of staphylococcin t (stt) staphylococcus cohnii at 37 degrees c after 24 and 48 hours incubation. ciprofloxacin in concentration of 0.25 microg/ml after 24 hours incubation inhibits antistaphylococcal activity stt produced by s. cohnii, while after 48 hour incubation, s. cohnii stt is excreted on the same level, in the presence and in the absence of ciprofloxacin.200919780489
layer-by-layer coating of bacteria with noble metal nanoparticles for surface-enhanced raman scattering.a simple layer-by-layer method to coat the bacterial cells with gold and silver nanoparticles (aunps and agnps) for the acquisition of surface-enhanced raman scattering (sers) spectra is reported. first, the bacteria cell wall is coated with poly (allylamine hydrochloride) (pah), a positively charged polymer, and then with citrate reduced au or agnps. in order to increase the stability of the coating, another layer of pah is prepared on the surface. the sem and afm images indicate that the nanop ...200919795108
[detection of meca gene in oxacillin-resistant coagulase-negative staphylococci isolated from the saliva of nursing professionals].coagulase-negative staphylococci are frequently associated with nosocomial infections, and healthcare professionals can be reservoirs and spread them in hospitals and in the community. the aim of this study was to identify species of coagulase-negative staphylococci isolated from the saliva of nursing professionals, determine the resistance profile and detect the meca gene. one hundred coagulase-negative staphylococci were selected: 41 were identified as staphylococcus epidermidis, 25 as staphyl ...200919802475
evaluation of matrix-assisted laser desorption ionization-time-of-flight mass spectrometry in comparison to rpob gene sequencing for species identification of bloodstream infection staphylococcal isolates.as a result of variable expression of biochemical characters, misidentification by conventional phenotypic means often occurs with clinical isolates belonging to staphylococcus species. therefore, we evaluated the use of matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (maldi-tof ms) for the identification of 450 blood isolates of the most relevant staphylococcal species, using sequence analysis of the rpob gene as the reference method. a correct species identificatio ...201120132252
maldi-tof mass spectrometry speciation of staphylococci and their discrimination from micrococci isolated from indoor air of schoolrooms.the focus of our work is the identification of medically relevant staphylococci from the environment; these organisms are among the major opportunistic pathogens associated with human disease. andersen sampling was performed in schoolrooms during the school year. eleven of thirty six isolates (all gram-positive tetrads) were identified as staphylococci and 23 were characterized as micrococci. maldi-tof ms profiling was used as the first stage in the classification followed by standard biochemica ...201020383373
amino acid residues modulating the activities of staphylococcal glutamyl endopeptidases.the glutamyl endopeptidase family of enzymes from staphylococci has been shown to be important virulence determinants of pathogenic family members, such as staphylococcus aureus. previous studies have identified the n-terminus and residues from positions 185-195 as potentially important regions that determine the activity of three members of the family. cloning and sequencing of the new family members from staphylococcus caprae (gluscpr) and staphylococcus cohnii (gluscoh) revealed that the n-te ...201020707600
rapid differentiation of staphylococcus aureus, staphylococcus epidermidis and other coagulase-negative staphylococci and meticillin susceptibility testing directly from growth-positive blood cultures by multiplex real-time pcr.this study evaluated a multiplex real-time pcr method specific for the meca, fema-sa and fema-se genes for rapid identification of staphylococcus aureus, staphylococcus epidermidis and non-s. epidermidis coagulase-negative staphylococci (cons), and meticillin susceptibility testing directly in positive blood cultures that grew gram-positive cocci in clusters. a total of 100 positive blood cultures produced: 39 s. aureus [12 meticillin-resistant s. aureus (mrsa), 31% of all the s. aureus]; 30 s. ...201020813851
culture-independent characterization of bacteria and fungi in a poultry bioaerosol using pyrosequencing: a new approach.work in animal production facilities often results in exposure to organic dusts. previous studies have documented decreases in pulmonary function and lung inflammation among workers exposed to organic dust in the poultry industry. bacteria and fungi have been reported as components of the organic dust produced in poultry facilities. to date, little is known about the diversity and concentration of bacteria and fungi inside poultry buildings. all previous investigations have utilized culture-base ...201021058154
coagulase-negative staphylococcus species in bulk milk: prevalence, distribution, and associated subgroup- and species-specific risk factors.coagulase-negative staphylococci (cns) have become the main pathogens causing bovine mastitis in recent years. a huge variation in species distribution among herds has been observed in several studies, emphasizing the need to identify subgroup- and species-specific herd-level factors to improve our understanding of the differences in ecological and epidemiological nature between species. the use of bulk milk samples enables the inclusion of a large(r) number of herds needed to identify herd-leve ...201727865514
characterization of a new sccmec element in staphylococcus cohnii.many sccmec elements of coagulase-negative staphylococci (cons) could not be typed using multiplex pcr. such a 'non-typable' sccmec was encountered in a staphylococcus cohnii isolate.201021103346
intrascrotal abscess, propionibacterium acnes and staphylococcus cohnii ssp. cohnii: a case report and review of the literature.introduction. the propionibacterium acnes and the staphylococcus cohnii ssp. cohnii are occasional pathogenic bacteria. the intrascrotal localization of the propionibacterium acnes is exceptional. the staphylococcus cohnii ssp. cohnii is not able to colonize the urogenital apparatus but it is the most frequently responsible for blood culture contamination even if it can sustain, in particular conditions, systemic infections. case presentation. we report the case of a 72-year-old man who is under ...201223227417
linezolid-resistant clinical isolates of enterococci and staphylococcus cohnii from a multicentre study in china: molecular epidemiology and resistance mechanisms.genetic characterisation of linezolid-resistant gram-positive cocci in a multicentre study in china has not been reported previously. to study the mechanism underlying the resistance of linezolid-resistant isolates, nine enterococcus faecalis, one enterococcus faecium and three staphylococcus cohnii isolates with various levels of resistance were collected from five hospitals across china in 2009-2012. the nine e. faecalis isolates were classified into seven sequence types, indicating that these ...201323880167
linezolid-resistant clinical isolates of meticillin-resistant coagulase-negative staphylococci and enterococcus faecium from china.seventeen meticillin-resistant coagulase-negative staphylococci (mrcons), including ten staphylococcus capitis, four staphylococcus cohnii, two staphylococcus haemolyticus and one staphylococcus sciuri, and an enterococcus faecium isolate with various levels of linezolid resistance were isolated from intensive care units in a chinese hospital. pfge indicated that the four s. cohnii isolates belonged to a clonal strain, and that nine of the s. capitis isolates were indistinguishable (clone a1) an ...201222899781
efficacy of silver/hydrophilic poly(p-xylylene) on preventing bacterial growth and biofilm formation in urinary catheters.catheter associated urinary tract infections (cauti), caused by several strains of bacteria, are a common complication for catheterized patients. this may eventually lead to a blockage of the catheter due to the formation of a crystalline or amorphous biofilm. inhibiting bacteria should result in a longer application time free of complaints. this issue has been investigated using an innovative type of silver-coated catheter with a semipermeable cap layer to prevent cauti. in this work, two diffe ...201728100054
in situ-monitoring of biofilm formation by using surface-enhanced raman scattering.a biofilm is a complex biochemical structure composed of microorganisms and extracellular polymeric substances used by microorganisms to adhere to each other and to surfaces. the monitoring of molecular changes during biofilm formation in situ can provide valuable insights in medicine, microbiology, and industrial processes. in this study, we investigated the characterization of biofilm produced by two model bacteria by using surface-enhanced raman scattering (sers) with the use of core silver ( ...201323643038
screening agars for mrsa: evaluation of a stepwise diagnostic approach with two different selective agars for the screening for methicillin-resistant staphylococcus aureus (mrsa).colonization with methicillin-resistant staphylococcus aureus (mrsa) poses a hygiene risk that does not spare field hospitals or military medical field camps during military deployments. diagnostic options for unambiguously identifying mrsa isolates are usually scarce in military environments. in this study, we assessed the stepwise application of two different selective agars for the specific identification of mrsa in screening analyses.201526199735
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