| distribution of staphylococcus species among human clinical specimens and emended description of staphylococcus caprae. | by dna-dna hybridization on microplates, we identified 1,230 strains of staphylococci from human clinical specimens and determined the distribution of species. the 10 staphylococcus species isolated most often were s. epidermidis (31.3%), s. aureus (23.3%), s. haemolyticus (12.2%), s. caprae (10.7%), s. simulans (4.4%), s. hominis (4.0%), s. capitis (3.9%), s. saprophyticus (3.6%), s. warneri (2.2%), and s. lugdunensis (1.3%). from these results, we realized that s. caprae strains were widely di ... | 1998 | 9650958 |
| tracking adhesion factors in staphylococcus caprae strains responsible for human bone infections following implantation of orthopaedic material. | ten staphylococcus caprae strains isolated from four patients and responsible for bone infections following implantation of orthopaedic material were compared to four s. caprae strains collected from milk samples of healthy goats. the following characteristics were investigated: smal patterns, hybridization patterns with pba2 (ribotypes), slime production, adhesion to matrix proteins (fibrinogen, fibronectin, collagen) and the staphylococcal adhesion genes (fnba, clfa, cna, atle, ica, fbe). none ... | 1999 | 10463169 |
| [a 72-year-old man with recurrent sepsis due to staphylococcus caprae]. | | 2000 | 11079232 |
| stability of microbial communities in goat milk during a lactation year: molecular approaches. | the microbial communities in milks from one herd were evaluated during 1-year of lactation, using molecular methods to evaluate their stability and the effect of breeding conditions on their composition. the diversity of microbial communities was measured using two approaches: molecular identification by 16s and 18s rdna sequencing of isolates from counting media (two milks), and direct identification using 16s rdna from clone libraries (six milks). the stability of these communities was evaluat ... | 2007 | 17604934 |
| staphylococcus caprae meningitis following intraspinal device infection. | a case is reported of staphylococcus caprae meningitis due to infection of an intraspinal analgesia pump. the subclinical and pauci-symptomatic clinical course of the infection strongly suggested a chronic device contamination. | 2008 | 18566153 |
| central line-associated bacteremia caused by drug-resistant staphylococcus caprae after chemotherapy for acute myelogenous leukemia. | | 2010 | 20390386 |
| identification of staphylococcus species by 16s-23s rdna intergenic spacer pcr analysis. | to investigate whether 16s-23s rdna (rdna) spacer region length polymorphisms are suitable for the identification of staphylococcus strains, the 16s-23s rdna intergenic spacer region lengths of 221 strains belonging to 31 species were studied by using a pcr-based method. each species presented a specific 16s-23s pattern made of 1-8 fragments ranging from 104-771 bp, with the exception of the species staphylococcus warnei, staphylococcus caprae and staphylococcus piscifermentans, which presented ... | 1998 | 9734063 |
| intra-articular empyema due to staphylococcus caprae following arthroscopic cruciate ligament repair. | staphylococcus caprae is a catalase-positive, coagulase-negative coccus that has been originally isolated from goat milk. we describe the first case of an intra-articular empyema caused by s. caprae in an immunocompetent patient following arthroscopic cruciate ligament repair. the patient recovered completely after debridement and antibiotic therapy with cefazolin and amoxicillin, respectively. this case demonstrates that this organism may rarely cause serious nosocomial infections in immunocomp ... | 1998 | 9733383 |
| ribotyping of staphylococcus caprae isolated from goat milk. | the usefulness of the api-staph system and a method based on ribosomal fingerprinting was evaluated by studying 89 coagulase-negative staphylococci isolated from goat milk. the bacteria were supposed to belong to the species staphylococcus caprae. the results obtained from the api-staph system showed variations in their phenotypic features. dna cleavage with ecori yielded six ribotypes and three distinct patterns were generated when the dna of the strains was digested with hindiii. forty-six str ... | 1998 | 9601147 |
| exoprotein and slime production by coagulase-negative staphylococci isolated from goats' milk. | milk from mastitis-free goats from french herds was examined for the presence of coagulase-negative staphylococci (cns), and 165 positive isolates were evaluated for their capacity to produce exoproteins. most isolates were identified as staphylococcus caprae (n = 91) or s. xylosus (n = 36), but members of at least nine other species were present. overall, some 57% of isolates produced toxins with phenotypic properties of alpha-hemolysin, and 75% produced toxins resembling s. aureus beta and/or ... | 1998 | 9549854 |
| isolation of staphylococcus caprae from blood cultures of a neonate with congenital heart disease. | | 1998 | 9512188 |
| human isolates of staphylococcus caprae: association with bone and joint infections. | staphylococcus caprae is a coagulase-negative, dnase-positive member of the genus staphylococcus usually associated with goats, but since 1991 a few laboratories have reported isolating the organism from human clinical specimens. we report on the isolation of 14 strains from human specimens and note that 10 strains were obtained from patients with bone and joint infections. nine of the 10 infections started with traumatic fractures, and the other was a case of mastoiditis. seven of these 10 infe ... | 1997 | 9316903 |
| comparison of the smai-digested chromosomes of staphylococcus epidermidis and the closely related species staphylococcus capitis and staphylococcus caprae. | pulsed-field gel electrophoresis was used to examine the chromosomal polymorphisms existing within and between four closely related members of the staphylococcus epidermidis species group, s. epidermidis, staphylococcus caprae, staphylococcus capitis subsp. capitis, and s. capitis subsp. ureolyticus. smai was chosen as the restriction endonuclease for this study because it generated only a few well-separated chromosomal fragments. each of the species and subspecies showed distinct smai digest pa ... | 1994 | 8068539 |
| identification and ribotypes of staphylococcus caprae isolates isolated as human pathogens and from goat milk. | we report five cases of human infection with staphylococcus caprae. two were community acquired (one case each of endocarditis and urinary tract infection); the other three were acquired in a hospital (two cases of bacteremia associated with intravenous access and one case of urinary tract infection). analysis of human isolates and goat isolates from eight herds showed that they could be misidentified by some commercial identification systems but were clearly identified as s. caprae by ribotypin ... | 1995 | 7790455 |
| udder infection of goats by coagulase-negative staphylococci. | infection of udder halves by coagulase-negative staphylococci in seven commercial goat herds was studied in conjunction with the california mastitis test (cmt). nine different species were identified and only 10% of strains belonged to groups which could not be identified with any of the known staphylococcus species. the most prevalent species were staphylococcus epidermidis (47.7%) and staphylococcus caprae (19.7%). about half of the coagulase-negative staphylococcal infections gave negative cm ... | 1984 | 6730303 |
| evaluation of the biotyper maldi-tof ms system for identification of staphylococcus species. | the bruker biotyper maldi-tof ms (biotyper) system, with a modified 30 minute formic acid extraction method, was evaluated by its ability to identify 216 clinical staphylococcus isolates from the cdc reference collection comprising 23 species previously identified by conventional biochemical tests. 16s rdna sequence analysis was used to resolve discrepancies. of these, 209 (96.8%) isolates were correctly identified: 177 (84.7%) isolates had scores ≥2.0, while 32 (15.3%) had scores between 1.70 a ... | 2015 | 26183765 |
| identification of a methicillin-resistant strain of staphylococcus caprae from a human clinical specimen. | the analysis of gel banding patterns of penicillin-binding proteins was used to identify two clinical isolates of a coagulase-negative staphylococcus species as staphylococcus caprae, a species originally isolated from goat's milk. one of the isolates was further shown to carry meca, the structural gene for methicillin resistance. | 1991 | 2014973 |
| identification of methicillin-resistant strains of staphylococci by polymerase chain reaction. | a simple and reliable method using a polymerase chain reaction (pcr) was devised to identify methicillin-resistant staphylococci. by using lysates of the strain to be tested as templates and 22-mer oligonucleotides as primers, a 533-bp region of meca, the structural gene of a low-affinity penicillin-binding protein (pbp 2'), was amplified by pcr and detected by agarose gel electrophoresis. results obtained by this method were compared with those obtained by broth microdilution mic determination ... | 1991 | 1939577 |
| amino acid residues modulating the activities of staphylococcal glutamyl endopeptidases. | the glutamyl endopeptidase family of enzymes from staphylococci has been shown to be important virulence determinants of pathogenic family members, such as staphylococcus aureus. previous studies have identified the n-terminus and residues from positions 185-195 as potentially important regions that determine the activity of three members of the family. cloning and sequencing of the new family members from staphylococcus caprae (gluscpr) and staphylococcus cohnii (gluscoh) revealed that the n-te ... | 2010 | 20707600 |
| whole-genome sequence of multidrug-resistant staphylococcus caprae strain 9557, isolated from cerebrospinal fluid. | staphylococcus caprae strain 9557 was isolated from a cerebrospinal fluid sample. the assembled genome contained 2,747,651-bp nucleotides with 33.34% gc content. consistent with its phenotypic characteristics, the genome harbors a varying repertoire of putative virulence factors involved in invasion, survival, and growth in the host cells. | 2015 | 26139720 |
| endophthalmitis following pars plana vitrectomy for vitreous floaters. | a case of staphylococcus caprae endophthalmitis in a young patient following pars plana vitrectomy for symptomatic vitreous floaters is reported here. recent literature suggests that there is an increasing trend of performing pars plana vitrectomy for symptomatic floaters. although rare, the potential risk of endophthalmitis should be explicitly discussed with patients considering surgical intervention for vitreous floaters. | 2014 | 25210434 |
| polymerase chain reaction detection of enterotoxins genes in coagulase-negative staphylococci isolated from brazilian minas cheese. | for a long time, staphylococcus aureus has been always thought to be the only pathogenic species among staphylococcus, while coagulase-negative staphylococci (cns) were classified as contaminant agents. however, molecular techniques have shown that these microorganisms also possess enterotoxin-encoding genes. the aim of this study was to analyze the frequency of genes for staphylococcal enterotoxins sea, seb, sec, and sed in cns strains isolated from minas soft cheese and to assess the in vitro ... | 2010 | 20528174 |
| report of a strain of staphylococcus caprae with the genes for enterotoxin a and enterotoxin-like toxin type p. | | 2007 | 17670927 |
| [staphylococcus caprae arthritis]. | | 2013 | 23398974 |
| subclinical mastitis and antimicrobial susceptibility of staphylococcus caprae and staphylococcus epidermidis isolated from two italian goat herds. | a total of 156 goats from 2 commercial dairy goat farms were monitored for intramammary infections during an entire lactation. most of the infections (80.7%) observed were due to coagulase-negative staphylococci (cns) species. in herd 1, nearly all of the infections (96%) were due to cns species, with staphylococcus caprae (scap) being the most common specific pathogen observed, accounting for about 43% of the infections. in herd 2, the proportion of the infections due to cns was 67% and staphyl ... | 2005 | 15829660 |
| identification of coagulase-negative staphylococci other than staphylococcus epidermidis by automated ribotyping. | as routine identification of coagulase-negative staphylococci is problematic, the performance of automated ribotyping was evaluated for identification of coagulase-negative staphylococci other than staphylococcus epidermidis. in total, 177 isolates were tested, comprising 149 isolates from blood samples, 15 isolates that were not identified by internal transcribed spacer (its)-pcr in a previous study, and 13 reference strains. the identification results were compared with those obtained by the a ... | 2005 | 15715714 |
| methicillin-resistant staphylococcus caprae in a neonatal intensive care unit. | staphylococcus caprae, a hemolytic coagulase-negative staphylococcus that is infrequently associated with humans, was initially detected in specimens from six infants in our neonatal intensive care unit due to phenotypic characteristics common to methicillin-resistant staphylococcus aureus. these isolates were subsequently identified as s. caprae by the automated riboprinter microbial characterization system. this prompted an 8-month retrospective investigation in our neonatal intensive care uni ... | 2005 | 15634995 |
| effect of subclinical intramammary infection on somatic cell counts, nagase activity and gross composition of goats' milk. | the study was aimed at identifying the pathogens causing subclinical udder infections in representative israeli dairy goat herds and determining their effect on milk quality. five hundred goats in ten flocks of various breeds and crossbreeds were surveyed. of the 500 goats, 13.4% were in their first lactation, 36.4% were in their second lactation and 50.2% were in their third or higher lactation. percentages of udder halves with subclinical intramammary infection in the flocks ranged from 35 to ... | 2004 | 15354577 |
| antibiotic susceptibility of coagulase-negative staphylococci isolated from goats' milk. | the antibiotic susceptibility of 70 strains of coagulase-negative staphylococci (cns) isolated during the 2001 lactating period from the milk of dairy goats, was evaluated. the antibiotics tested were benzylpenicillin, cloxacillin, amoxicillin, amoxicillin plus clavulanic acid, cephalonium and cefoperazone, erythromycin and tylmicosin, kanamycin and tetracycline. minimum inhibitory concentration (mic) measurements showed that all beta-lactams (except cefoperazone) were effective against staphylo ... | 2004 | 15194138 |
| effect of intramammary infection by staphylococcus caprae on somatic cell counts and milk composition in goats. | | 2002 | 12222809 |
| microbiology of acute otitis externa. | to isolate and characterize bacteria and fungi from acute otitis externa (aoe) and to obtain susceptibility profiles on each bacterial isolate. | 2002 | 12169893 |
| several regions of the repeat domain of the staphylococcus caprae autolysin, atlc, are involved in fibronectin binding. | the autolysin atlc is the only known fibronectin-binding protein in staphylococcus caprae strain 96007. the fibronectin-binding domain of atlc consists of three repeats (atlcr(1)r(2)r(3)), which are located between the two enzymatic domains. the atlcr(1)r(2)r(3) domain and the atlcr(1)r(2) and atlcr(3) subdomains were expressed separately as his(6)-tagged proteins. in western affinity blots, only atlcr(1)r(2)r(3) and atlcr(3) but not atlcr(1)r(2) appeared to recognise fibronectin; however, in el ... | 2002 | 12167537 |
| staphylococcus caprae strains carry determinants known to be involved in pathogenicity: a gene encoding an autolysin-binding fibronectin and the ica operon involved in biofilm formation. | the atlc gene (1,485 bp), encoding an autolysin which binds fibronectin, and the ica operon, involved in biofilm formation, were isolated from the chromosome of an infectious isolate of staphylococcus caprae and sequenced. atlc (155 kda) is similar to the staphylococcal autolysins atl, atle, aas (48 to 72% amino acid identity) and contains a putative signal peptide of 29 amino acids and two enzymatic centers (n-acetylmuramoyl-l-alanine amidase and endo-beta-n-acetylglucosaminidase) interconnecte ... | 2001 | 11159959 |
| [infection after total hip replacement by staphylococcus caprae. case report and review of the literature]. | we report a case of staphylococcus caprae bone and joint infection, that illustrate difficulties to diagnose coagulase-negative staphylococci (cns) orthopedic surgery infections, specially following implantation of prostheses. four of 5 strains successivelly isolated from deep and/or peri-operative specimens during late infection after total hip replacement (thr) have been identified, using commercial systems and conventionnal tests, as s. caprae. identity of biochemical profile, antibiotype and ... | 1999 | 10418010 |
| differentiation of staphylococcus spp. by high-resolution melting analysis. | high-resolution melting analysis (hrma) is a fast (post-pcr) high-throughput method to scan for sequence variations in a target gene. the aim of this study was to test the potential of hrma to distinguish particular bacterial species of the staphylococcus genus even when using a broad-range pcr within the 16s rrna gene where sequence differences are minimal. genomic dna samples isolated from 12 reference staphylococcal strains (staphylococcus aureus, staphylococcus capitis, staphylococcus caprae ... | 2010 | 21164574 |
| propeptide processing and proteolytic activity of proenzymes of the staphylococcal and enterococcal gluv8-family protease. | proenzymes with various lengths of propeptides have been observed in gluv8 from staphylococcus aureus and gluse from s. epidermidis. however, the production mechanism of these proenzymes and roles of truncated propeptides have yet to be elucidated. here we demonstrate that shortening of propeptide commonly occurs in an auto-catalytic manner in gluv8-family members, including those from coagulase negative staphylococci and enterococcus faecalis. accompanied with propeptide shortening, the pro-mat ... | 2012 | 23350276 |
| identification of staphylococcus spp. using (gtg)₅-pcr fingerprinting. | a group of 212 type and reference strains deposited in the czech collection of microorganisms (brno, czech republic) and covering 41 staphylococcus species comprising 21 subspecies was characterised using rep-pcr fingerprinting with the (gtg)₅ primer in order to evaluate this method for identification of staphylococci. all strains were typeable using the (gtg)₅ primer and generated pcr products ranging from 200 to 4500 bp. numerical analysis of the obtained fingerprints revealed (sub)species-spe ... | 2010 | 21095086 |
| staphylococcus caprae bone and joint infections: a re-emerging infection? | staphylococcus caprae has been recently classified as a human pathogen, but the incidence of s. caprae in human bone and joint infections (bjis) is under-reported. in this study, we report 25 cases of s. caprae bji, and we review the 31 cases published in the literature. molecular techniques and matrix-assisted laser desorption ionization time-of-flight mass spectrometry improved the identification of clinically relevant s. caprae strains. in this study, 96% of s. caprae bjis were localized to t ... | 2014 | 24975594 |
| use of a staphylococcal vaccine to reduce prevalence of mastitis and lower somatic cell counts in a registered saanen dairy goat herd. | this investigation evaluated the efficacy of a bacterin in reducing the prevalence of staphylococcal mastitis and somatic cell counts (scc) in a dairy goat herd. does were vaccinated or left as controls, and the levels of mastitis and scc monitored over 18 months. staphylococcus caprae (42.5%), s. xylosus (15.1%), and s. simulans (10.0%) were the predominant causes of intramammary infections (imi). the infection rate was 1.64 imi/doe among vaccinates, which tended to be lower (p < 0.12) than con ... | 2014 | 24837996 |
| short communication: identification of coagulase-negative staphylococcus species from goat milk with the api staph identification test and with transfer rna-intergenic spacer pcr combined with capillary electrophoresis. | coagulase-negative staphylococci (cns) are the most commonly isolated bacteria from goat milk, but they have often been identified with phenotypic methods, which may have resulted in misclassification. the aims of this paper were to assess the amount of misclassification of a phenotypic test for identifying cns species from goat milk compared with transfer rna intergenic spacer pcr (tdna-pcr) followed by capillary electrophoresis, and to apply the tdna-pcr technique on different capillary electr ... | 2012 | 23040022 |
| differences between coagulase-negative staphylococcus species in persistence and in effect on somatic cell count and milk yield in dairy goats. | coagulase-negative staphylococci (cns) are the most commonly isolated bacteria from goat milk. the goal of this study was to explore and describe differences between cns species in persistence of intramammary infection (imi) and in effect on somatic cell count (scc) and milk yield (my). milk samples were collected from 530 does from 5 dutch dairy goat herds on 3 occasions during 1 lactation. coagulase-negative staphylococci species were identified at the species level by transfer rna-intergenic ... | 2012 | 22916911 |
| matrix-assisted laser desorption ionization time of flight mass spectrometry and diagnostic testing for prosthetic joint infection in the clinical microbiology laboratory. | identification of pathogen(s) associated with prosthetic joint infection (pji) is critical for patient management. historically, many laboratories have not routinely identified organisms such as coagulase-negative staphylococci to the species level. the advent of matrix-assisted laser desorption ionization time of flight mass spectrometry (maldi-tof ms) has enhanced clinical laboratory capacity for accurate species-level identification. the aim of this study was to describe the species-level ide ... | 2015 | 25533615 |
| effect of stage of lactation on the immune competence of goat mammary gland. | the present research was undertaken to evaluate the effect of stage of lactation on immune competence of the goat mammary gland. the prevalence and etiology of intramammary infections and cytokine pattern were studied in individual caprine milk during lactation. a total of 1,200 caprine milk samples were collected during early, mid, and late lactation (less than 40 d, from 110 to 130 d, and more than 200 d in lactation, respectively) and analyzed for milk quality, bacterial cell load, and cytoki ... | 2016 | 26947302 |
| antimicrobial activity of ceftaroline and comparator agents when tested against numerous species of coagulase-negative staphylococcus causing infection in us hospitals. | a total of 1593 coagulase-negative staphylococci (cons) considered clinically significant were collected from 71 us medical centers in 2013-2014 and tested for susceptibility by clsi broth microdilution methods. species identification was performed by matrix-assisted laser desorption ionization-time-of-flight mass spectrometry. overall, 59.7% of isolates were oxacillin resistant (mrcons). ceftaroline (mic50/90, 0.25/0.5μg/ml) inhibited 99.2% of cons at ≤1μg/ml (susceptible breakpoint for staphyl ... | 2016 | 26971182 |
| bilateral one-stage revision of infected total hip arthroplasties: report of two cases and management of antibiotic therapy. | recommendations for the management of chronic and bilateral total hip arthroplasty (tha) infection are lacking. however, this type of infection involves medical problems concerning the management of the antibiotic therapy. we report two cases of such infections operated as one-stage revision. for each case, both hips were infected with the same bacteria (staphylococcus caprae for one patient and methicillin-sensitive staphylococcus aureus for the other). the probabilistic antibiotic treatment st ... | 2016 | 26904335 |
| characterization of staphylococcus caprae clinical isolates involved in human bone and joint infections, compared with goat mastitis isolates. | staphylococcus caprae is an emerging microorganism in human bone and joint infections (bji). the aim of this study is to describe the features of s. caprae isolates involved in bji (h for human) compared with those of isolates recovered in goat mastitis (a for animal). fourteen isolates of each origin were included. identifications were performed using a vitek 2 gp id card, tuf gene sequencing, and matrix-assisted laser desorption ionization-time of flight (maldi-tof) vitek ms. molecular typing ... | 2016 | 26511738 |
| coagulase-negative staphylococci: pathogenesis, occurrence of antibiotic resistance genes and in vitro effects of antimicrobial agents on biofilm-growing bacteria. | coagulase-negative staphylococci (cons) are opportunistic pathogens that particularly cause infections in patients with implanted medical devices. the present research was performed to study the virulence potential of 53 clinical isolates of staphylococcus capitis, staphylococcus auricularis, staphylococcus lugdunensis, staphylococcus simulans, staphylococcus cohnii and staphylococcus caprae. all clinical strains were clonally unrelated. isolates carried genes encoding resistance to β-lactam (me ... | 2016 | 27902368 |
| staphylococcus caprae native mitral valve infective endocarditis. | staphylococcus caprae is a rare cause of infective endocarditis. here, we report a case involving the native mitral valve in the absence of an implantable cardiac electronic device. | 2016 | 28348787 |
| virulence and toxigenicity of coagulase-negative staphylococci in nigerian traditional fermented foods. | the incidence of coagulase-negative staphylococci (cons) may render food unsafe, as the clinical isolates have been reported to exude virulent traits. a total of 255 cons isolates from 6 traditional fermented foods (nono, kunu, wara, iru, ogi, and kindirmo) from north central nigeria, identified as staphylococcus epidermidis, staphylococcus simulans, staphylococcus xylosus, staphylococcus kloosii, and staphylococcus caprae, were investigated for virulence traits. the strains were examined for bi ... | 2016 | 27192326 |