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susceptibility of staphylococcus species and subspecies to fleroxacin.twenty-four staphylococcus species or subspecies were examined for their susceptibilities to the fluoroquinolone fleroxacin (ro 23-6240) by disk diffusion (5-micrograms disk) and by agar dilution for the determination of mics. resistant strains were further tested for their susceptibilities to oxacillin and the fluoroquinolone ciprofloxacin. reference strains of the novobiocin-resistant species (staphylococcus saprophyticus, staphylococcus cohnii, staphylococcus xylosus, staphylococcus arlettae, ...19911759838
susceptibility of staphylococcus species and subspecies to teicoplanin.twenty-four staphylococcus species and their subspecies were examined for their susceptibilities to teicoplanin by disk diffusion (30-micrograms disk) and agar dilution for the determination of mics. moderately susceptible and resistant clinical strains were further tested for their susceptibilities to oxacillin and vancomycin. teicoplanin resistance was not observed in the reference strains of the various staphylococcus species isolated from healthy volunteers or animals. however, the novobioci ...19911835340
extremely halotolerant bacteria characteristic of fully cured and dried cod.gram-positive cocci were isolated in high numbers from salted codfish during processing. they were found to be the main bacterial type in fully cured and dried salted cod. phenotypic characterization of 37 strains showed them to belong to the novobiocin resistant staphylococci, most likely staphylococcus arlettae or xylosus. based on sequencing of 16s rdna and comparison of 700 bases it was concluded that they should be assigned to the species staphylococcus arlettae. they were found to be extre ...19979217105
staphylococcus nepalensis sp. nov., isolated from goats of the himalayan region.four coagulase-negative, novobiocin-resistant cocci, designated cw1(t), pm34, mm3 and rw78, were isolated from the respiratory tract of goats kept in the himalayan region. the four isolates were assigned to a single species on the basis of almost identical biochemical and physiological traits, protein profiles obtained after sds-page and identical genomic fingerprints generated after enterobacterial repetitive intergenic consensus (eric)-pcr. strain cw1(t) showed highest 16s rdna sequence simila ...200314657137
evaluation of three different molecular markers for the detection of staphylococcus aureus by polymerase chain reaction.the aim of this study was to target three genes of staphylococcus aureus-fmha (coding for a factor of unknown function), catalase and fema (coding for a factor essential for methicillin resistance) to establish and validate a pcr assay for the detection of this pathogen. two pairs of primers were designed for fmha and one pair each for catalase and fema genes. the pcr assays were standardized and found to give specific amplicons under similar reaction parameters. target specificity of the primer ...200818355670
inhibition of map kinases and down regulation of tnf-alpha, il-beta and cox-2 genes by the crude extracts from marine bacteria.crude ethyl acetate extracts from marine bacterial isolates staphylococcus arlettae kp2 (genbank accession no. eu594442) and planococcus maritimus kp8 (genbank accession no. eu594443) isolated from andaman seas were studied for their anti-inflammatory effect by lymphocyte proliferation assay (lpa) employing peripheral blood mononuclear cells (pbmcs). the crude extracts from both the bacteria down regulated the synthesis of inflammatory mediators such as tumor necrosis factor-alpha (tnf-alpha), i ...200918996678
exposure to culturable airborne bioaerosols during noodle manufacturing in central taiwan.biological hazards associated with the manufacturing of noodles have not been well characterized in taiwan. this is an issue that flour workers can be exposed to bioaerosols (airborne fungi and bacteria) resulting flour-induced occupational asthma or allergic diseases. this study is to survey the species and concentrations of bioaerosols at different sites within a noodle factory for one year, and to investigate the effects of environmental factors on concentrations of bioaerosols. air samples w ...200919062076
evaluation of microbial dynamics during the ripening of a traditional taiwanese naturally fermented ham.isolation and identification of the autochthonous starter from a naturally fermented meat allows control of the fermentation process and promises microbiological safety for this specialty. thus the purpose of this study was to identify the lactic acid bacteria and coagulase-negative cocci present in a traditional taiwanese naturally fermented ham (tnfh) and to study the microbial dynamics at different ripening stages; the approach was a combination of conventional microbiological cultivation, po ...201020417394
hexavalent chromium reduction and plant growth promotion by staphylococcusarlettae strain cr11.cr(vi), a mutagenic and carcinogenic pollutant in industrial effluents, was effectively reduced by an indigenous tannery effluent isolate staphylococcus arlettae strain cr11 under aerobic conditions. the isolate could tolerate cr(vi) up to 2000 and 5000mgl(-1) in liquid and solid media respectively. s. arlettae cr11 effectively reduced 98% of 100mgl(-1) cr(vi) in 24h. reduction for initial cr(vi) concentrations of 500 and 1000mgl(-1) was 98% and 75%, respectively in 120h. the isolate was also po ...201122169713
cow teat skin, a potential source of diverse microbial populations for cheese production.the diversity of the microbial community on cow teat skin was evaluated using a culture-dependent method based on the use of different dairy-specific media, followed by the identification of isolates by 16s rrna gene sequencing. this was combined with a direct molecular approach by cloning and 16s rrna gene sequencing. this study highlighted the large diversity of the bacterial community that may be found on teat skin, where 79.8% of clones corresponded to various unidentified species as well as ...201222081572
evaluation of a new lipase from staphylococcus sp. for detergent additive capability.lipases are the enzymes of choice for laundry detergent industries owing to their triglyceride removing ability from the soiled fabric which eventually reduces the usage of phosphate-based chemical cleansers in the detergent formulation. in the present study, a partially purified bacterial lipase from staphylococcus arlettae jpbw-1 isolated from the rock salt mine has been assessed for its triglyceride removing ability by developing a presoak solution so as to use lipase as an additive in laundr ...201324106703
evaluation of muscodor cinnamomi as an egg biofumigant for the reduction of microorganisms on eggshell surfaces and its effect on egg quality.the presence of microorganisms on the eggshell surface is a factor of consideration in determining egg quality. these microorganisms can contribute to egg spoilage and can infect the egg. in this study, 18 morphotypes of microorganisms were isolated from eggshells. morphological, biochemical, physiological and molecular analyses were used to identify these morphotypes into 7 species; bacillus drentensis, staphylococcus arlettae, stap. cohnii, stap. kloosii, stap. saprophyticus, stap. sciuri and ...201728068588
bioprospection of bacteria and yeasts from atlantic rainforest soil capable of growing in crude-glycerol residues.the increasing world production of biodiesel has resulted in an accumulation of crude glycerol as the major byproduct. this could be used as carbon source for industrial microbiology, with economic and environmental advantages for the biodiesel industry. we explored an atlantic rainforest soil sample to search for crude glycerol-degrading microorganisms. microcosms of this soil were established containing minimal medium + 8% crude glycerol (w/w); the biological activity was measured by respirome ...201324222222
biodiversity and phylogenetic analysis of culturable bacteria indigenous to khewra salt mine of pakistan and their industrial importance.culturable bacterial biodiversity and industrial importance of the isolates indigenous to khewra salt mine, pakistan was assessed. pcr amplification of 16s rdna of isolates was carried out by using universal primers fd1 and rp1and products were sequenced commercially. these gene sequences were compared with other gene sequences in the genbank databases to find the closely related sequences. the alignment of these sequences with sequences available from genbank database was carried out to constru ...200824031194
assessing the bioremediation potential of arsenic tolerant bacterial strains in rice rhizosphere interface.the arsenic tolerant bacterial strains staphylococcus arlettae (nbrieag-6), staphylococcus sp. (nbrieag-8) and brevibacillus sp. (nbrieag-9) were tested for their roles in enhancing plant growth and induction of stress-related enzymes in rice (oryza sativa l. cv. ndr-359) plants at two different concentrations, 30 and 15mg/kg of as(v) and as(iii), respectively. an experiment was conducted to test the effect of these strains on plant growth promotion and arsenic uptake. we found 30%-40% reduction ...201627745656
bacteriophage immobilized graphene electrodes for impedimetric sensing of bacteria (staphylococcus arlettae).bacteriophages are a class of viruses that specifically infect and replicate within a bacterium. they possess inherent affinity and specificity to the particular bacterial cells. this property of bacteriophages makes them an attractive biorecognition element in the field of biosensor development. in this work, we report the use of an immobilized bacteriophage for the development of a highly sensitive electrochemical sensor for staphylococcus arlettae, bacteria from the pathogenic family of coagu ...201627114042
the diversities of staphylococcal species, virulence and antibiotic resistance genes in the subclinical mastitis milk from a single chinese cow herd.staphylococci are the leading pathogens of bovine mastitis which is difficult to control. however, the published data on the prevalence of staphylococcal species, virulence and antibiotic resistance genes in bovine mastitis from china are limited. in this study, 104 out of 209 subclinical mastitis milk samples from a single chinese dairy herd were cultured-positive for staphylococci (49.8%), which were further identified as coagulase-positive staphylococci (cps) or coagulase-negative staphylococ ...201526276706
modelling and optimization studies on a novel lipase production by staphylococcus arlettae through submerged fermentation.microbial enzymes from extremophilic regions such as hot spring serve as an important source of various stable and valuable industrial enzymes. the present paper encompasses the modeling and optimization approach for production of halophilic, solvent, tolerant, and alkaline lipase from staphylococcus arlettae through response surface methodology integrated nature inspired genetic algorithm. response surface model based on central composite design has been developed by considering the individual ...201324455210
an investigation of resistance to β-lactam antimicrobials among staphylococci isolated from pigs with exudative epidermitis.a high proportion of staphylococci isolated from pigs affected with exudative epidermitis were found to be resistant to β-lactam antimicrobials. the primary objective of this research was to investigate and characterize β-lactam resistance in staphylococcus hyicus, staphylococcus aureus and other staphylococci isolated from these pigs.201324131819
production and characterization of a halo-, solvent-, thermo-tolerant alkaline lipase by staphylococcus arlettae jpbw-1, isolated from rock salt mine.studies on lipase production and characterization were carried out with a bacterial strain staphylococcus arlettae jpbw-1 isolated from rock salt mine, darang, hp, india. higher lipase activity has been obtained using 10 % inoculum with 5 % of soybean oil as carbon source utilizing a ph 8.0 in 3 h at 35 °c and 100 rpm through submerged fermentation. partially purified s. arlettae lipase has been found to be active over a broad range of temperature (30-90 °c), ph (7.0-12.0) and nacl concentration ...201323955348
characterization of an extremely halotolerant staphylococcus arlettae hpssn35c isolated from dwarka beach, india.an extremely halotolerant bacterium designated as hpssn35c was isolated from saline soil of dwarka beach, india. it exhibited growth over a wide range of nacl in medium varying from 0 to 6 m. the isolate produced peach-pink pigment above ∼1.3 m nacl. the culture was characterized using biochemical tests, biomerieux staph identification kit, api id32 staph system, and biolog. due to slow growth and extreme salt tolerance no id was obtained in biolog. antibiotic sensitivity to various antibiotics ...201423828308
genome sequence of staphylococcus arlettae strain cvd059, isolated from the blood of a cardiovascular disease patient.we have isolated a staphylococcus arlettae strain, strain cvd059, from the blood of a rheumatic mitral stenosis patient. here, we report the genome sequence and potential virulence factors of this clinical isolate. the draft genome of s. arlettae cvd059 is 2,565,675 bp long with a g+c content of 33.5%.201223144377
influence of inoculation of arsenic-resistant staphylococcus arlettae on growth and arsenic uptake in brassica juncea (l.) czern. var. r-46.an arsenic hypertolerant bacterium was isolated from arsenic contaminated site of west bengal, india. the bacteria was identified as staphylococcus arlettae strain nbrieag-6, based on 16s rdna analysis. s. arlettae was able to remove arsenic from liquid media and possesses arsc gene, gene responsible for arsenate reductase activity. the biochemical profiling of the isolated strain showed that it had the capacity of producing indole acetic acid (iaa), siderophores and 1-aminocyclopropane-1-carbox ...201322939092
distribution of non-aureus staphylococci species in udder quarters with low and high somatic cell count, and clinical mastitis.the effect of non-aureus staphylococci (nas) in bovine mammary health is controversial. overall, nas intramammary infections (imi) increase somatic cell count (scc), with an effect categorized as mild, mostly causing subclinical or mild to moderate clinical mastitis. however, based on recent studies, specific nas may affect the udder more severely. some of these apparent discrepancies could be attributed to the large number of species that compose the nas group. the objectives of this study were ...201728456402
prevalence of non-aureus staphylococci species causing intramammary infections in canadian dairy herds.non-aureus staphylococci (nas), the microorganisms most frequently isolated from bovine milk worldwide, are a heterogeneous group of numerous species. to establish their importance as a group, the distribution of individual species needs to be determined. in the present study, nas intramammary infection (imi) was defined as a milk sample containing ≥1,000 cfu/ml in pure or mixed culture that was obtained from a cohort of cows assembled by the canadian bovine mastitis research network. overall, 6 ...201728527793
novel β-lactamase blaarl in staphylococcus arlettae.whole-genome sequencing of penicillin-resistant staphylococcus arlettae strain san1670 from bovine mastitis milk revealed a novel β-lactamase operon consisting of the β-lactamase-encoding gene blaarl, the antirepressor-encoding gene blar1arl, and the repressor-encoding gene blaiarl. the functionality of blaarl was demonstrated by gene expression in staphylococcus aureus. the blaarl operon was chromosomally located in san1670 and present in 10 additional unrelated strains, suggesting intrinsic pe ...201728497118
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