| establishment of eubacterium cellulosolvens in the digestive tract of axenic and meroxenic mice: influence of feed cellulose content. | the cellulolytic bacterial species present in the caecum and colon contents of conventionally reared mature mice did not become established in the digestive tract when inoculated to axenic mice, whatever the size of the inoculum or the cellulose content of the diet. the cellulolytic bacterium eubacterium cellulosolvens sc 10 isolated from mouse digestive flora was unable to become established in the digestive tract of axenic mice whatever the cellulose content of the diet; it requires a feed ric ... | 1991 | 1855647 |
| a comparison of strains of eubacterium cellulosolvens from the rumen. | | 1972 | 4537442 |
| effects of lasalocid or monensin on lactate-producing or -using rumen bacteria. | lasalocid or monensin inhibited most of the lactate-producing rumen bacteria (butyrivibrio fibrisolvens, eubacterium cellulosolvens, e. ruminantium, lachnospira multiparus, lactobacillus ruminis, l. vitulinus, ruminococcus albus, r. flavefaciens, streptococcus bovis). minimum inhibitory concentrations ranged from .38 to 3.0 micrograms/ml. among the lactate producers, those that produce succinate as a major end product (bacteroides, selenomonas, succinimonas, succinivibrio) were not inhibited by ... | 1981 | 7275867 |
| regulation of the cellulolytic activity of eubacterium cellulosolvens 5494: a review. | eubacterium cellulosolvens 5494 is a cellulolytic gram positive bacterium isolated from the rumen. substrate specific regulation has not been previously demonstrated in any members of this genera. however, we have recently found that e. cellulosolvens regulates some of its membrane proteins. growth on different substrates, including cellulose and cellobiose, gave different sds-page profiles of proteins from the membrane fraction. using scanning electron microscopy, we also found that growth of e ... | 1996 | 8652133 |
| 16s rdna analysis of butyrivibrio fibrisolvens: phylogenetic position and relation to butyrate-producing anaerobic bacteria from the rumen of white-tailed deer. | complete 16s rdna sequences of six strains of butyrivibrio fibrisolvens, including the type strain (atcc 19171), were determined. the type strain was found to have less than 89% sequence similarity to the other isolates that were examined. the five plasmid-bearing strains formed a closely related cluster and three of these strains (ob156, ob157 and ob192) were very highly related (> 99%), indicating that they are isolates of the same genomic species. the phylogenetic position of butyrivibrio was ... | 1996 | 8987694 |
| genetic manipulation of anaerobic cellulolytic bacteria. | transposon tn1545 was introduced into the chromosome of the ruminal cellulolytic bacterium eubacterium cellulosolvens. this was achieved by conjugal transfer of the transposon from clostridium beijerinckii at a frequency of about 1 per 10(4) recipient cells. transconjugants of e. cellulosolvens were resistant to both tetracycline and erythromycin. e. cellulosolvens could also serve as a donor for conjugal transfer of tn1545 back into c. beijerinckii. | 1997 | 9274059 |
| conjugal transfer of transposon tn1545 into the cellulolytic bacterium eubacterium cellulosolvens. | tn1545, a self-mobilizing transposon, was introduced into the chromosome of the ruminal cellulolytic bacterium eubacterium cellulosolvens. this was achieved by conjugal transfer of the transposon from clostridium beijerinckii at a frequency of 1 per 10(6) recipient cells. transconjugants of eu. cellulosolvens were resistant to both tetracycline and erythromycin, and were able to mobilize tn1545 back into cl. beijerinckii. southern blot hybridization of representative transconjugants did not reve ... | 1998 | 9489031 |
| cloning, nucleotide sequence and expression of the gene encoding the cellulose-binding protein a (cbpa) of eubacterium cellulosolvens 5. | the nucleotide sequence of the gene encoding the cellulose-binding protein a (cbpa) of eubacterium cellulosolvens 5 was determined. the gene consists of an open reading frame of 3453 nucleotides and encodes a protein of 1151 amino acids with a molecular mass of 126408 da. the deduced amino acid sequence of cbpa contained one domain highly similar to a catalytic domain of glycosyl hydrolases belonging to family 9, two linker-like domains and four domains of unknown function. among the four domain ... | 2002 | 11958931 |
| identification of the cellulose-binding and the cell wall-binding domains of eubacterium cellulosolvens 5 cellulose-binding protein a (cbpa). | the cellulose-binding domain (cbd) and the cell wall-binding domain (cwbd) of eubacterium cellulosolvens 5 cellulose-binding protein a (cbpa) have been determined. the gene (cbpa) encoding cbpa and its derivatives were expressed in escherichia coli. we were able to obtain the eight recombinant proteins and examine for their cellulose-binding ability, cell wall-binding ability and carboxymethyl cellulase (cmcase) activity. since five recombinant proteins, which contain the unknown domain (ud-2) l ... | 2002 | 12204381 |
| presence of several cellulose-binding proteins in culture supernatant and cell lysate of eubacterium cellulosolvens 5. | attempts were made to separate and characterize cellulose-binding proteins (cbps) from both the culture supernatant and cell lysate of eubacterium cellulosolvens 5. once the cbps were bound to avicel cellulose, they were then effectively eluted with the solution containing 3.2 or 5% sodium dodecyl sulfate (sds), but not eluted with the solution containing various kinds of carbohydrates and reagents. namely, cbps in both the culture supernatant and cell lysate of the bacterium bound tightly and s ... | 2001 | 12483607 |
| a possible role of cellulose-binding protein a (cbpa) in the adhesion of eubacterium cellulosolvens 5 to cellulose. | the cellulose-binding protein a (cbpa) of eubacterium cellulosolvens 5 is a modular enzyme comprised of a catalytic domain, a cellulose-binding domain and a cell wall-binding domain. cellobiose-grown cells changed their adhesion ability to cellulose depending on the growth phase. on the other hand, carboxymethyl cellulose (cmc)-grown cells bound to cellulose regardless of their growth phase. the distribution of cbpa in the culture supernatant and cell fractions changed depending on the carbon so ... | 2003 | 14581993 |
| cloning, sequencing, and expression of a eubacterium cellulosolvens 5 gene encoding an endoglucanase (cel5a) with novel carbohydrate-binding modules, and properties of cel5a. | a novel eubacterium cellulosolvens 5 gene encoding an endoglucanase (cel5a) was cloned and expressed in escherichia coli, and its enzymatic properties were characterized. the cel5a gene consists of a 3,444-bp open reading frame and encodes a 1,148-amino-acid protein with a molecular mass of 127,047 da. cel5a is a modular enzyme consisting of an n-terminal signal peptide, two glycosyl hydrolase family 5 catalytic modules, two novel carbohydrate-binding modules (cbms), two linker sequences, and a ... | 2005 | 16204489 |
| cloning, nucleotide sequence and module structure of the gene encoding the cellulose-binding protein b (cbpb) of eubacterium cellulosolvens 5. | the nucleotide sequence of the gene encoding the cellulose-binding protein b (cbpb) of eubacterium cellulosolvens 5 was determined. the gene consists of an open reading frame of 3,429 nucleotides. the deduced amino acid sequence of cbpb contained one module highly similar to a catalytic module of glycosyl hydrolase family 9 (ghf9), one module partially similar to a family 3 carbohydrate-binding module (cbm3), two linkers, one module similar to a cbm of cellulose-binding protein a (cbpa) from e. ... | 2005 | 16205028 |
| biochemical characterization of cellulose-binding proteins (cbpa and cbpb) from the rumen cellulolytic bacterium eubacterium cellulosolvens 5. | the cellulose-binding proteins, cbpa and cbpb, of rumen cellulolytic bacterium eubacterium cellulosolvens 5 were biochemically characterized, and their properties were compared. recombinant cbpa and cbpb were a typical 1,4-beta-endoglucanase. both proteins bound to insoluble polysaccharides such as avicel cellulose, acid swollen cellulose, lichenan, chitin, and oat spelt xylan. on the other hand, only recombinant cbpb bound to agarose and starch. | 2007 | 17928689 |
| transfer of conjugative elements from rumen and human firmicutes bacteria to roseburia inulinivorans. | studies on firmicutes bacteria from the gut are hampered by a lack of gene transfer systems. here the human colonic anaerobe roseburia inulinivorans a2-194 was shown to be a transfer recipient for two conjugative transposons, tn1545 from eubacterium cellulosolvens and tnk10 from clostridium saccharolyticum k10. | 2008 | 18456856 |
| cloning and sequencing of the gene for cellobiose 2-epimerase from a ruminal strain of eubacterium cellulosolvens. | cellobiose 2-epimerase (ce; ec 5.1.3.11) is known to catalyze the reversible epimerization of cellobiose to 4-o-beta-d-glucopyranosyl-d-mannose in ruminococcus albus cells. here, we report a ce in a ruminal strain of eubacterium cellulosolvens for the first time. the nucleotide sequence of the ce had an orf of 1218 bp (405 amino acids; 46 963.3 da). the ce from e. cellulosolvens showed 44-54% identity to n-acyl-d-glucosamine 2-epimerase-like hypothetical proteins in the genomes of coprococcus eu ... | 2008 | 18710396 |
| overproduction, purification, crystallization and preliminary x-ray characterization of a novel carbohydrate-binding module of endoglucanase cel5a from eubacterium cellulosolvens. | the anaerobic cellulolytic rumen bacterium eubacterium cellulosolvens produces a large array of cellulases and hemicellulases that are responsible for the hydrolysis of plant cell-wall polysaccharides. one of these enzymes, endoglucanase cel5a, comprises two tandemly repeated novel carbohydrate-binding modules (cbms) and two catalytic domains belonging to glycoside hydrolase family 5 joined by flexible linker sequences. the novel cbm located at the n-terminus of the endoglucanase has been crysta ... | 2011 | 21505249 |
| identification and functional expression of genes encoding flavonoid o- and c-glycosidases in intestinal bacteria. | gut bacteria play a crucial role in the metabolism of dietary flavonoids and thereby influence the bioactivity of these compounds in the host. the intestinal lachnospiraceae strain cg19-1 and eubacterium cellulosolvens are able to deglycosylate c- and o-coupled flavonoid glucosides. growth of strain cg19-1 in the presence of the isoflavone c-glucoside puerarin (daidzein 8-c-glucoside) led to the induction of two proteins (dfgc, dfgd). heterologous expression of the encoding genes (dfgc, dfgd) in ... | 2016 | 25845411 |
| molecular diversity of rumen bacterial communities from tannin-rich and fiber-rich forage fed domestic sika deer (cervus nippon) in china. | sika deer (cervus nippon) have different dietary preferences to other ruminants and are tolerant to tannin-rich plants. because the rumen bacteria in domestic sika deer have not been comprehensively studied, it is important to investigate its rumen bacterial population in order to understand its gut health and to improve the productivity of domestic sika deer. | 2013 | 23834656 |
| overproduction, purification, crystallization and preliminary x-ray characterization of the c-terminal family 65 carbohydrate-binding module (cbm65b) of endoglucanase cel5a from eubacterium cellulosolvens. | the rumen anaerobic cellulolytic bacterium eubacterium cellulosolvens produces a large range of cellulases and hemicellulases responsible for the efficient hydrolysis of plant cell wall polysaccharides. one of these enzymes, endoglucanase cel5a, comprises a tandemly repeated carbohydrate-binding module (cbm65) fused to a glycoside hydrolase family 5 (cel5a) catalytic domain, joined by flexible linker sequences. the second carbohydrate-binding module located at the c-terminus side of the endogluc ... | 2013 | 23385766 |
| understanding how noncatalytic carbohydrate binding modules can display specificity for xyloglucan. | plant biomass is central to the carbon cycle and to environmentally sustainable industries exemplified by the biofuel sector. plant cell wall degrading enzymes generally contain noncatalytic carbohydrate binding modules (cbms) that fulfil a targeting function, which enhances catalysis. cbms that bind β-glucan chains often display broad specificity recognizing β1,4-glucans (cellulose), β1,3-β1,4-mixed linked glucans and xyloglucan, a β1,4-glucan decorated with α1,6-xylose residues, by targeting s ... | 2013 | 23229556 |
| intestinal bacterium eubacterium cellulosolvens deglycosylates flavonoid c- and o-glucosides. | eubacterium cellulosolvens cleaved the flavone c-glucosides homoorientin and isovitexin to their aglycones luteolin and apigenin, respectively. the corresponding isomers, orientin and vitexin, or other polyphenolic c-glucosides were not deglycosylated. e. cellulosolvens also cleaved several o-coupled glucosides of flavones and isoflavones to their corresponding aglycones. | 2012 | 22961906 |
| characteristics of ruminal anaerobic celluloytic cocci and cillobacterium cellulosolvens n. sp. | | 1958 | 13598714 |