| assessment of anaerobic wastewater treatment failure using terminal restriction fragment length polymorphism analysis. | the suitability of genetic fingerprinting to study the microbiological basis of anaerobic bioreactor failure is investigated. | 2005 | 16313419 |
| methane- and sulfur-metabolizing microbial communities dominate the lost city hydrothermal field ecosystem. | hydrothermal venting and the formation of carbonate chimneys in the lost city hydrothermal field (lchf) are driven predominantly by serpentinization reactions and cooling of mantle rocks, resulting in a highly reducing, high-ph environment with abundant dissolved hydrogen and methane. phylogenetic and terminal restriction fragment length polymorphism analyses of 16s rrna genes in fluids and carbonate material from this site indicate the presence of organisms similar to sulfur-oxidizing, sulfate- ... | 2006 | 16957253 |
| archaeal communities in high arctic wetlands at spitsbergen, norway (78 degrees n) as characterized by 16s rrna gene fingerprinting. | emissions of the greenhouse gas methane from arctic wetlands have been studied extensively, though little is known about the ecology and community structure of methanogenic archaea that catalyze the methane production. as part of a project addressing microbial transformations of methane in arctic wetlands, we studied archaeal communities in two wetlands (solvatnet and stuphallet) at spitsbergen, norway (78 degrees n) during two summer seasons. directly extracted peat community dna and enrichment ... | 2005 | 16329932 |
| response of archaeal communities in beach sediments to spilled oil and bioremediation. | while the contribution of bacteria to bioremediation of oil-contaminated shorelines is well established, the response of archaea to spilled oil and bioremediation treatments is unknown. the relationship between archaeal community structure and oil spill bioremediation was examined in laboratory microcosms and in a bioremediation field trial. 16s rrna gene-based pcr and denaturing gradient gel analysis revealed that the archaeal community in oil-free laboratory microcosms was stable for 26 days. ... | 2004 | 15128510 |
| effect of soil type and fertilizer management on archaeal community in upland field soils. | the effects of soil and fertilizer types on archaeal communities were evaluated by real-time pcr and pcr-denaturing gradient gel electrophoresis (dgge) targeting the 16s rrna gene of total dna directly extracted from upland field soils. twelve experimental upland field plots containing four different soil types, i.e., cumulic andosol, low-humic andosol, yellow soil and gray lowland soil, were maintained under three different fertilizer management systems for 8 years (chemical fertilizer, rice hu ... | 2011 | 21670564 |
| [spatial characteristics of microbial groups associated with the groundwater flow in a small watershed]. | few reports of microbial groups associated with the groundwater flow system are available in china. 16s rrna gene library was constructed by the cultured-independent approach to investigate gene sequences of microorganism in groundwater samples from the recharge (r), intermediate (m) and discharge (d) zones of an experimental watershed at zhuhai campus of sun yat-sen university. proteobacterium, candidate division opx, uncultured archaeon (uncultured crenarchaeote and euryarchaeote) and actinoba ... | 2010 | 21229735 |
| identification and quantitative evaluation of nitrogen-converting organisms in a full-scale leachate treatment plant. | the presence of ammonia nitrogen in landfill leachates poses a significant problem for treatment plant operators. the nitrification-denitrification process mostly carries out the nitrogen conversion in biological treatment systems. however, recent research shows that other processes by anaerobic ammonia-oxidizing bacteria (anammox) and ammonia-oxidizing archaea (aoa) were also responsible for the removal of nitrogen in biological systems. in this study, the nitrogen-converting microorganisms in ... | 2010 | 20974516 |
| bacterial and archaeal communities in the surface sediment from the northern slope of the south china sea. | microbial diversity of sediments from the northern slope of the south china sea was studied by constructing bacterial and archaeal 16s rrna gene clone libraries. fourteen bacterial phylogenetic groups were detected, including gammaproteobacteria, deltaproteobacteria, planctomycetes, alphaproteobacteria, firmicutes, bacteroidetes, actinobacteria, chloroflexi, acidobacteria, betaproteobacteria, nitrospirae, candidate divisions op8 and op11, and an unknown group. gammaproteobacteria was the predomi ... | 2009 | 19946953 |
| distribution of crenarchaeota representatives in terrestrial hot springs of russia and iceland. | culture-independent (pcr with crenarchaeota-specific primers and subsequent denaturing gradient gel electrophoresis) and culture-dependent approaches were used to study the diversity of crenarchaeota in terrestrial hot springs of the kamchatka peninsula and the lake baikal region (russia) and of iceland. among the phylotypes detected there were relatives of both cultured (mainly hyperthermophilic) and uncultured crenarchaeota. it was found that there is a large and diverse group of uncultured cr ... | 2008 | 18849450 |
| high abundance of crenarchaeota in a temperate acidic forest soil. | the objective of the study was to elucidate the depth distribution and community composition of archaea in a temperate acidic forest soil. numbers of archaea and bacteria were measured in the upper 18 cm of the soil, and soil cores were sampled on two separate occasions using quantitative pcr targeting 16s rrna genes. maximum numbers of archaea were 0.6-3.8 x 10(8) 16s rrna genes per gram of dry soil. numbers of bacteria were generally higher, but archaea always accounted for a high percentage o ... | 2007 | 17391330 |
| accessing the black box of microbial diversity and ecophysiology: recent advances through polyphasic experiments. | the microbial ecology of a range of anaerobic biological assemblages (granular sludge) from full- and laboratory-scale wastewater treatment bioreactors, and of crop-growing and peat soils, was determined using a variety of 16s rrna gene-based techniques, including clone library, terminal restriction fragment length polymorphism (trflp) and denaturing gradient gel electrophoresis (dgge) analyses. fluorescent in situ hybridization (fish) using 16s rrna gene-targeted probes was employed to complete ... | 2006 | 16702066 |