| antigens of selected acanthamoeba species detected with monoclonal antibodies. | acanthamoeba species are ubiquitous soil and freshwater protozoa that have been associated with infections of the human brain, skin, lungs and eyes. our aim was to develop specific antibodies to aid in rapid and specific diagnosis of clinically important isolates. mice were variously immunised with live mixtures of acanthamoeba castellanii strain 112 (ac112) trophozoites and cysts, or with sonicated, formalin-fixed or heat-treated trophozoites, or with a trophozoite membrane preparation. eight h ... | 2005 | 15964573 |
| fatal disseminated acanthamoeba lenticulata infection in a heart transplant patient. | we report a fatal case of disseminated acanthamebiasis caused by acanthamoeba lenticulata (genotype t5) in a 39-year-old heart transplant recipient. the diagnosis was based on skin histopathologic results and confirmed by isolation of the ameba from involved skin and molecular analysis of a partial 18s rrna gene sequence (df3). | 2007 | 17553253 |
| potentially human pathogenic acanthamoeba isolated from a heated indoor swimming pool in switzerland. | some free-living amoebae, including some species of the genus acanthamoeba, can cause infections in humans and animals. these organisms are known to cause granulomatous amebic encephalitis (gae) in predominantly immune-deficient persons. in the present study, we isolated a potentially human pathogenic acanthamoeba isolate originating from a public heated indoor swimming pool in switzerland. the amoebae, thermophilically preselected by culture at 37 degrees c, subsequently displayed a high thermo ... | 2009 | 19041307 |
| viability of listeria monocytogenes in co-culture with acanthamoeba spp. | listeria monocytogenes is a human pathogen, ubiquitous in the environment, and can grow and survive under a wide range of environmental conditions. it contaminates foods via raw materials or food-processing environments. however, the current knowledge of its ecology and, in particular, the mode of environmental survival and transmission of this intracellular pathogen remains limited. research has shown that several intracellular pathogens are able to survive or replicate within free-living amoeb ... | 2009 | 19645820 |
| cooccurrence of free-living amoebae and nontuberculous mycobacteria in hospital water networks, and preferential growth of mycobacterium avium in acanthamoeba lenticulata. | the incidence of lung and other diseases due to nontuberculous mycobacteria (ntm) is increasing. ntm sources include potable water, especially in households where ntm populate pipes, taps, and showerheads. ntm share habitats with free-living amoebae (fla) and can grow in fla as parasites or as endosymbionts. fla containing ntm may form cysts that protect mycobacteria from disinfectants and antibiotics. we first assessed the presence of fla and ntm in water and biofilm samples collected from a ho ... | 2013 | 23475613 |
| acanthamoeba lenticulata keratitis in a hard contact lens wearer. | | 2013 | 23448683 |
| legionella oakridgensis atcc 33761 genome sequence and phenotypic characterization reveals its replication capacity in amoebae. | legionella oakridgensis is able to cause legionnaires' disease, but is less virulent compared to l. pneumophila strains and very rarely associated with human disease. l. oakridgensis is the only species of the family legionellae which is able to grow on media without additional cysteine. in contrast to earlier publications, we found that l. oakridgensis is able to multiply in amoebae. we sequenced the genome of l. oakridgensis type strain or-10 (atcc 33761). the genome is smaller than the other ... | 2013 | 23932911 |
| pathogenic waterborne free-living amoebae: an update from selected southeast asian countries. | data on the distribution of free-living amoebae is still lacking especially in southeast asian region. the aquatic environment revealed a high occurrence of free-living amoebae (fla) due to its suitable condition and availability of food source, which subsequently causes infection to humans. a total of 94 water samples consisted of both treated and untreated from laos (31), myanmar (42), and singapore (21) were investigated for the presence of pathogenic fla. each water sample was filtered and c ... | 2017 | 28212409 |
| diversity and seasonal impact of acanthamoeba species in a subtropical rivershed. | this study evaluated the presence of acanthamoeba species in the puzih river watershed, which features typical subtropical monsoon climate and is located just above the tropic of cancer in taiwan. the relationship between the seasonal and geographical distributions of acanthamoeba species in this rivershed was also investigated. acanthamoeba species were detected in water samples using the amoebal enrichment culture method and confirmed by pcr. a total of 136 water samples were included in this ... | 2013 | 24490160 |
| reevaluation of an acanthamoeba molecular diagnostic algorithm following an atypical case of amoebic keratitis. | amoebic keratitis (ak) is a potentially blinding infection, the prompt diagnosis of which is essential for limiting ocular morbidity. we undertook a quality improvement initiative with respect to the molecular detection of acanthamoebae in our laboratory because of an unusual case of discordance. nine atcc strains of acanthamoeba and 40 delinked, biobanked, surplus corneal scraping specimens were analyzed for the presence of acanthamoebae with four separate real-time pcr assays. the assay used b ... | 2015 | 26202123 |
| drug-resistant severe acanthamoeba keratitis caused by rare t5 acanthamoeba genotype. | to describe a case of severe and drug-resistant acanthamoeba keratitis in a contact lens wearer caused by atypical t5 acanthamoeba genotype (acanthamoeba lenticulata). | 2010 | 20461886 |
| balamuthia and acanthamoeba-binding antibodies in west african human sera. | little is known about the prevalence of balamuthia mandrillaris amoebae and balamuthia amoebic encephalitis in africa. as an approach, relative concentrations of amoebae-binding serum antibodies (ab) were assessed by flow cytometry using formaldehyde-fixed b. mandrillaris, acanthamoeba lenticulata 72-2 and acanthamoeba castellanii 1bu amoebae for specific ab capture (b.m.-ab, a.l.-ab, a.c.-ab). one hundred and ninety-two sera from west african (côte d'ivoire) donors aged 11-95years (mean 38 a; 5 ... | 2010 | 19896940 |
| acute granulomatous acanthamoeba encephalitis in an immunocompetent patient. | acanthamoeba sp. are known to cause fatal granulomatous acanthamoeba encephalitis (gae) in immunocompromised patients. | 2010 | 19847677 |
| assessment of balamuthia mandrillaris-specific serum antibody concentrations by flow cytometry. | a flow cytometry (fluorescence-activated cell sorter)-based assay was adapted to detect and quantify antibodies to balamuthia mandrillaris, a causative agent of fatal amoebic encephalitis (bae), and to acanthamoeba species. with sera from bae patients for positive and a group of inconspicuous volunteers for negative reference, most of the 237 sera from random blood donors, patients with atypical encephalitis, atypical pneumonitis, visceral amoebiasis and toxoplasmosis and from subjects working w ... | 2009 | 19039606 |
| genotyping of pathogenic acanthamoebae isolated from clinical samples in greece--report of a clinical isolate presenting t5 genotype. | amoebae belonging to the genus acanthamoeba are potentially pathogenic to humans, causing mainly amoebic keratitis. pathogenic ability of the 15 known acanthamoeba genotypes is under investigation. we report that four out of five cases of amoebic keratitis studied in greece, present t4 sequence type, while the remaining one presents t5 sequence type (acanthamoeba lenticulata), which is the second most frequent genotype found among environmental samples. thus, it is confirmed, for the first time ... | 2006 | 16490381 |
| obligate bacterial endosymbionts of acanthamoeba spp. related to the beta-proteobacteria: proposal of 'candidatus procabacter acanthamoebae' gen. nov., sp. nov. | all obligate bacterial endosymbionts of free-living amoebae currently described are affiliated with the alpha-proteobacteria, the chlamydiales or the phylum cytophaga-flavobacterium-bacteroides. here, six rod-shaped gram-negative obligate bacterial endosymbionts of clinical and environmental isolates of acanthamoeba spp. from the usa and malaysia are reported. comparative 16s rdna sequence analysis demonstrated that these endosymbionts form a novel, monophyletic lineage within the beta-proteobac ... | 2002 | 11931173 |
| different growth rates in amoeba of genotypically related environmental and clinical legionella pneumophila strains isolated from a thermal spa. | two cases of legionellosis occurring 3 years apart were acquired in the same french thermal spa and were apparently due to the same strain of legionella pneumophila serogroup 1, as shown by genomic macrorestriction analysis. minor differences between the two isolates were found by random amplification pcr profiling which showed an additional band with one of the isolates. analysis of 107 l. pneumophila strains isolated from the spa waters by genome macrorestriction failed to identify the infecti ... | 2001 | 11349974 |
| group-i introns with unusual sequences occur at three sites in nuclear 18s rrna genes of acanthamoeba lenticulata. | seven of eleven isolates of acanthamoeba lenticulata were found to have group-i introns located at one of three positions within the 18s rrna gene. the introns are 636-721-bp long and are absent from mature rrna. they lack open reading frames that could encode any known endonucleases. sequences of introns from the same site in different isolates are 86.0-98.9% identical, while from different sites they are 24.2-29.8% identical. the most closely related introns from other organisms are in the 18s ... | 1998 | 9683678 |
| mycobacterium bovis hosted by free-living-amoebae permits their long-term persistence survival outside of host mammalian cells and remain capable of transmitting disease to mice. | bovine tuberculosis (tb) is a zoonotic disease caused by mycobacterium bovis. despite intensive tb control campaigns, there are sporadic outbreaks of bovine tb in regions declared tb free. it is unclear how m. bovis is able to survive in the environment for long periods of time. we hypothesized that free-living amoebae (fla), as ubiquitous inhabitants of soil and water, may act as long-term reservoirs of m. bovis in the environment. in our model, m. bovis would be taken up by amoebal trophozoite ... | 2017 | 28585299 |