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acanthamoeba griffini. molecular characterization of a new corneal pathogen.acanthamoeba was isolated from the cornea of a soft contact lens wearer who had keratitis. the protozoan was also isolated from the contact lens storage case and the domestic water supply used to clean the case. using morphologic features, all three isolates were identified tentatively as a. griffini, a species not previously associated with keratitis. complete small subunit ribosomal rna gene (18s rdna) sequence analysis was used to characterize further the three isolates.19968595954
subgenus systematics of acanthamoeba: four nuclear 18s rdna sequence types.classification of acanthamoeba at the subgenus level has been problematic, but increasing reports of acanthamoeba as an opportunistic human pathogen have generated an interest in finding a more consistent basis for classification. thus, we are developing a classification scheme based on rna gene sequences. this first report is based on analysis of complete sequences of nuclear small ribosomal subunit rna genes (rns) from 18 strains. sequence variation was localized in 12 highly variable regions. ...19968976608
phylogenetic relationships among acanthamoeba spp. based on pcr-rflp analyses of mitochondrial small subunit rrna gene.we investigated the value of mitochondrial small subunit rrna gene (mt ssu rdna) pcr-rflp as a taxonomic tool for acanthamoeba isolates with close inter-relationships. twenty-five isolates representing 20 species were included in the analysis. as in nuclear 18s rdna analysis, two type strains (a. astronyxis and a. tubiashi) of morphological group 1 diverged earliest from the other strains, but the divergence between them was less than in 18s riboprinting. acanthamoeba griffini of morphological g ...199910507226
length and sequence heterogeneity in the mitochondrial internal transcribed spacer of acanthamoeba spp.the internal transcribed spacer (its) between the mitochondrial large (23s rrna; rnl) and small (16s rrna; rns) subunit ribosomal rna genes of acanthamoeba castellanii strain neff was sequenced previously and was uniquely interesting because it contained trna genes with acceptor stem mismatches that underwent rna editing repair. our interest in this its region was to determine its phylogenetic potential in differentiating between closely related isolates. we analyzed the mitochondrial its region ...200919527353
comparison of potentially pathogenic free-living amoeba hosts by legionella spp. in substrate-associated biofilms and floating biofilms from spring environments.this study compares five genera of free-living amoebae (fla) hosts by legionella spp. in the fixed and floating biofilm samples from spring environments. detection rate of legionella spp. was 26.9% for the floating biofilms and 3.1% for the fixed biofilms. acanthamoeba spp., hartmanella vermiformis, and naegleria spp. were more frequently detected in floating biofilm than in fixed biofilm samples. the percentage of pathogenic acanthamoeba spp. among all the genus acanthamoeba detected positive s ...201121831404
genotyping of acanthamoeba t15: the environmental strain in turkey.environmental sources are potential sources for the transmission of acanthamoeba in humans and other mammals.201525424836
intra-amoebal killing of mycobacterium ulcerans by acanthamoeba griffini: a co-culture model.mycobacterium ulcerans, a decaying mycobacterium marinum derivative is responsible for buruli ulcer, a notifiable non-contagious disabling infection highly prevalent in some west african countries. aquatic environments are suspected to host m. ulcerans, however, the exact reservoirs remain unknown. while m. marinum was found to resist amoebal microbicidal activities, this remains unknown for m. ulcerans. in this study m. ulcerans was co-cultured with the moderately halophile acanthamoeba griffin ...201729155010
morphological features and in vitro cytopathic effect of acanthamoeba griffini trophozoites isolated from a clinical case.light and transmission electron microscopy observations are reported on the structure and in vitro cytopathic effect of acanthamoeba griffini trophozoites isolated from a clinical case. live trophozoites were moderately active with a remarkable pleomorphism which changed from ovoid to quite elongated shapes. when moving, amoebae formed cytoplasmic projections such as wide lamellae and acanthopodia of diverse size and thickness which contain a significant amount of actin. ultrastructurally, the c ...201425313337
characterization of a human-pathogenic acanthamoeba griffini isolated from a contact lens-wearing keratitis patient in spain.amoebae were isolated from contact lenses of a symptomatic lens wearer in spain. protozoa were characterized by studying their morphology, biology, protease activity and the 18s rrna gene sequence. morphology of the organism was observed by light microscopy, scanning electron microscopy and transmission electron microscopy. its structure corresponded to an amphizoic amoeba. the protozoa grew well at 37 °c and poorly at lower temperatures. in addition, it was capable of lysing mammalian cells in ...201525068502
thermotolerant acanthamoeba spp. isolated from therapeutic hot springs in northwestern iran.this study was conducted to address the distribution of acanthamoeba genotypes in therapeutic hot springs in iran. sixty water and sediment samples were collected from bicarbonate, sulphur, and sodium chloride thermal springs in the northwest. all hot springs examined are used mainly for health purposes in iran. acanthamoeba were identified by both morphology and pcr (polymerase chain reaction). genotype identification was based on the sequencing of a highly variable and informative region of di ...201223165720
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