| [glycogen synthetase and fructose diphosphatase activity in the tussues of mollusks (mytilus galloprovincialis) and crustacenas (balanus improvisus, carcinus maenas)]. | the level of the activity of the enzymes studied is rather similar in all the species investigated, being the highest in muscles and gills. seasonal changes in the activity of the enzymes in mussel tissues are closely related to reproductive periods and reflect changes in the level of glycogen synthesis. | 1976 | 185853 |
| [anionic detergents in mytilus galloprovincialis of the gulf of naples]. | the authors have investigated on the anionics detergents rate inside the mytilus galloprovincialis, used as animal-monitor of the environment state of a certain part of the gulf of naples. the data concerning 14 zones in which were drown mussels cultivated in the gulf of naples resumed in a specific list, shown an high concentration of above detergents. | 1979 | 554639 |
| evaluation of heavy metal pollution in the venetian lagoon by using mytilus galloprovincialis as biological indicator. | this study reports the analysis of heavy metal (cd, co, cu, cr, hg, fe, mn, ni, pb, zn) and as accumulated in the mollusc mytilus galloprovincialis collected in the venetian lagoon between march and september 1988. this environmental biomonitoring project was carried out using natural population of the molluscs attached to the 'briccole', which limit the navigation canals into the lagoon. these data were compared with those reported by other authors in analogous studies published about a decade ... | 1992 | 1631531 |
| occurrence of faecal bacteria, salmonella and antigens associated with hepatitis a virus in shellfish. | an investigation was carried out over a one year period to examine jointly the occurrence of faecal bacteria, salmonella and the presence of antigens associated with the hepatitis a virus (hav) in oysters (crassostrea gigas), mussels (mytilus edulis, mytilus galloprovincialis) and cockles (cerastoderma edule), taken from 8 shellfish farming areas or natural beds along the french coast. for the faecal coliforms (fc) and faecal streptococci (fs), statistical analysis of the 176 samples examined sh ... | 1991 | 1663750 |
| mixed-function oxygenase enzymes as tools for pollution monitoring: field studies on the french coast of the mediterranean sea. | 1. mfo enzyme activities were measured in microsomes from whole mussels (mytilus galloprovincialis) comber livers (serranus cabrilla), or posidonia oceanica etiolated tissues, and pah contents were determined in sediments collected in coastal locations of the french riviera and corsica during 3 oceanographic cruises in 1987-1988. 2. bap activities in mussel and erod activities measured in fish were strongly correlated to the log of pah content in sediments. the first results for ca4h in posidoni ... | 1991 | 1677868 |
| chlamydia-like organisms in digestive and duct cells of mussels from the basque coast. | chlamydia-like organisms have been detected in digestive cells and duct cells of the digestive gland of mussels, mytilus galloprovincialis, collected from the basque coast. the organisms appeared as basophilic inclusion bodies within digestive cells and consisted of elongate initial reticulate bodies and previously undescribed condensed forms, interpreted as intermediate bodies. of the 414 mussels examined by light microscopy, 5.31% showed this type of infection. a second type of chlamydia-like ... | 1991 | 1787326 |
| a rapid method for detecting dna strand breaks in mytilus galloprovincialis lam. induced by genotoxic xenobiotic chemicals. | 1. in this study, dna from haemolymph cells of mytilus galloprovincialis lam., as well as from l1210 (murine leukemia) mouse cells was investigated utilizing the technique of the alkaline unwinding of the double stranded dna molecule. 2. the data show that dna of haemolymph cells from the marine invertebrate has an unwinding time and, therefore, a molecular weight considerably lower than that of dna of mammalian cells. 3. the exposure of the cells from mussel haemolymph and from mouse l1210 to a ... | 1991 | 1999266 |
| induction of micronuclei by mitomycin c and colchicine in the marine mussel mytilus galloprovincialis. | the frequencies of micronuclei (mni) in the gill cells of the mussel mytilus galloprovincialis were determined over a long period (up to 28 days) following a 48-h treatment with colchicine. the frequency of mni at the end of treatment was significantly higher than in controls and 24 h later it had increased even more. after this period, the frequency of mni rapidly declined until a plateau level was reached on day 2-3, which was significantly higher than the control baseline level, and persisted ... | 1990 | 2113181 |
| depuration of mytilus galloprovincialis experimentally contaminated with hepatitis a virus. | mussels (mytilus galloprovincialis) were contaminated with known amounts of laboratory strains of hepatitis a virus and poliovirus 1 and the effectiveness of a self-cleansing mechanism was studied using a pilot depuration system. both viruses were rapidly bioaccumulated by mussels and the maximal concentration of about 10(4) tcid50/ml was reached within 1.5 hours. depuration was carried out up to 24 h; infectivity titer decreased to 10(2) tcid50/ml and 10(3.2) tcid50/ml within 6 h in hepatitis a ... | 1990 | 2177996 |
| isolation and biochemical characterization of the microsomal fraction from the digestive gland of mussel mytilus galloprovincialis lam. | a procedure to prepare microsomes from the mussel digestive gland is proposed. the data concerning the biochemical characterization of this subcellular fraction shows a typical rna:protein ratio, but the presence of hydrolytic enzymes was also found; therefore a mixture of hydrolase inhibitors to study the different biochemical characteristics was used. the biochemical data demonstrate that glucose-6-phosphatase activity (g6pase), a typical microsomal marker in mammalian cells, is not present in ... | 1986 | 2423292 |
| mercury, cadmium and lead levels in marine organisms (mytilus galloprovincialis lmk.) collected along the italian coasts. | the growing importance assumed in the last ten years by contamination of the marine environment caused by heavy metals has evidenced the necessity to constantly deepen the knowledge of pollutant concentration that can reach man through the food chain. particularly interesting is the determination of potentially toxic elements in marine organisms which through accumulation and concentration processes can provide a direct correlation with the ecosystem's degree of contamination. the present study ... | 1989 | 2624360 |
| selective activation of carcinogenic aromatic amines to bacterial mutagens in the marine mussel mytilus galloprovincialis. | the postmitochondrial fraction of the marine mussel mytilus galloprovincialis digestive gland activates selectively precarcinogenic aromatic amines, but not precarcinogenic benzo[a]pyrene, to salmonella typhimurium ta 98 mutagens. this activation potential is nadph-dependent, is not inducible by exposure to diesel 2 oil and a polluted environment, and is inhibited by methimazole. the characteristics of this activation potential are consistent with the recent finding of the presence of fad-contai ... | 1986 | 2877776 |
| metabolic activation of 2-aminofluorene, 2-acetylaminofluorene and n-hydroxy-acetylaminofluorene to bacterial mutagens with mussel (mytilus galloprovincialis) and carp (cyprinus carpio) subcellular preparations. | 1. the mode of activation of 2-aminofluorene (af), 2-acetylaminofluorene (aaf) and n-hydroxy-acetylaminofluorene (oh-aaf) to salmonella typhimurium ta 98 mutagens was investigated in subcellular fractions from the digestive gland of the mussel mytilus galloprovincialis and from the liver of carp cyprinus carpio. 2. in carp liver microsomes the activation of oh-aaf was due to very active deacetylase, in contrast to undetectable deacetylase-dependent activation in mussel microsomes. 3. af and aaf ... | 1987 | 2890481 |
| gaba-like immunoreactivity in the pedal ganglia of mytilus galloprovincialis: light and electron microscopic study. | the distribution of gamma-aminobutyric acid (gaba)-like immunoreactivity was investigated in the pedal ganglia (pg) of mytilus galloprovincialis (mollusca, bivalvia) with the aid of an antiserum raised against gaba coupled to bovine serum albumin. examination of whole-mount preparations and serial vibratome and semithin sections showed the presence of different types of immunoreactive neurons. small unipolar neurons were the most numerous, and were located mainly in the lateral ganglion cortex. ... | 1988 | 3346374 |
| principal component analysis for the identification of pollution sources in mussel survey by trace metals. | principal component analysis has been applied to analyze the correlation matrix obtained from a 8 x 43 data matrix. the 8 trace metals are mn, co, ni, cu, zn, cd, hg, pb, which are contained in the soft part of mussels (mytilus galloprovincialis lamarck). mussels were sampled from two sites in the gulf of trieste. in both samples, 76-78% of the total variance is explained by the four principal components. the orthogonally rotated factor matrix indicates that co and ni are bonded to the first pri ... | 1987 | 3564700 |
| [ultrastructural analysis of secretory cells and hyaline hemocytes with a "neoplastic" character in mytilus galloprovincialis (lamarck) collected from a natural polluted site (bay of fos)]. | the comparative ultrastructural analysis of the digestive tubules of mussels sampled in a strongly hydrocarbons polluted neritic area and in another one reputed unpolluted, revealed important adulterations in the first one, namely that cells of digestive tubules and hyalinocytes within digestive gland display "neoplastic" disorders. | 1985 | 3933776 |
| an h1-like protein from the sperm chromatin of mytilus galloprovincialis. | we have isolated and purified a sperm-specific protein (s3) from the mussel m. galloprovincialis. antibodies against s3 were raised in rabbits and used for its immunological comparison to somatic histones. the results showed that s3 did not share common immunological determinants with h2b or any other core histone-contrary to the suggestion that it was an h2b-like protein (ausio and subirana, 1982). with h1 there was a crossreaction between s3 and anti-h1 as well as with h1 and anti-s3. although ... | 1985 | 3986920 |
| metabolism in the rat of cadmium biocomplexes from edible mussel exposed to 109cdcl2. | the metabolism in the rat of 109cd biocomplexes present in the tissues of the edible mussel mytilus galloprovincialis, previously exposed to 109cdcl2, was studied. the tissue distribution and binding of 109cd were compared to those caused by an equal dose of 109cd as cdcl2 or rat liver cd-metallothionein. administration of mussel 109cd to rats resulted in an initial accumulation of 109cd in the kidneys due to the presence of 109cd-metallothionein, which constituted 25% of the 109cd in the tissue ... | 1985 | 3987600 |
| [isolation of yershinia enterocolitica in mytilus galloprovincialis lamk]. | | 1968 | 5735741 |
| [different shell development in mytilus galloprovincialis in 2 different populations from the gulf of naples]. | the size frequency distribution, the shell weight, the semilogarythmic growth, the carbonic anhydrase activity has been investigated between two populations of mussels from different sampling zones. significative differences were found in the parameters investigated between the two populations. the authors conclude that the reason of this morphological and biochemical differences may arise from different natural hydrobiological factors and/or from different pollution of their habitat. | 1983 | 6411100 |
| heavy metals at trace level in edible mussels (mytilus galloprovincialis lamarck) from the gulf of trieste. | the concentration of heavy metals (cobalt, nickel, copper, cadmium, mercury and lead), which are present at trace levels in the edible part of mussels (mytilus galloprovincialis lamarck) from hatcheries in the gulf of trieste, is normally or log-normally distributed, according to a statistical test sensitive to asymmetry of the distribution. a log-normal distribution appears valid for describing nearly all trace metals, in particular toxic heavy metals like cadmium, mercury and lead. the frequen ... | 1984 | 6495868 |
| principal component analysis as a tool for studying interdependences among trace metals in edible mussels from the gulf of trieste. | principal component analysis has been applied to analyze the correlation matrix obtained from a (6 x 44) data matrix. the 6 trace metals are co, ni, cu, cd, hg, and pb, which are contained in the soft part of edible mussels (mytilus galloprovincialis lamarck) from a hatchery on the gulf of trieste. in the space of "eigenvectors", the dimensions are reduced to 3 principal axes, which account for about 77% of the total variance. the unrotated factor matrix obtained by the principal components show ... | 1984 | 6524079 |
| [accumulation of heavy metals in biocrystals in the shell of mytilus galloprovincialis from different zones of the gulf of naples]. | the amounts of fe, zn, cu, mg were determined in the inner and outer layers of shells of mytilus g.. different values significatively between the layers were found only for mg. the ratio [me]calcite/ [me]aragonite was calculated and it is not more than one, for all the colonies of different sampling zones. the authors conclude that perhaps the reason of this behaviour is the different pollution of their habitat. | 1983 | 6882546 |
| analysis for polycyclic aromatic hydrocarbons in mussels (mytilus galloprovincialis) from the thermaikos gulf, greece. | | 1982 | 7093552 |
| a mitochondrial dna discontinuity in the mussel mytilus galloprovincialis lmk: pleistocene vicariance biogeography and secondary intergradation. | | 1995 | 7739394 |
| the effects of culture-site, depth, season, and stock source on the prevalence of marteilia refringens in cultured mussels (mytilus galloprovincialis lmk.) from galicia, spain. | samples of a marteiliad parasite in mytilus galloprovincialis lmk. from nw spain were identified as marteilia refringens, using light and electron microscopy. the most important lesions in the mussel tissues were found in the digestive tubules at the moment of the release of the sporangia, resulting in a breakage of the epithelial cells of the digestive tubules. the plasmodia of a marteilia-like organism were also found in the gills, accompanied by a severe host reaction in 1 of the 4,200 examin ... | 1995 | 7776120 |
| comparison of mouse bioassay, hplc and enzyme immunoassay methods for determining diarrhetic shellfish poisoning toxins in mussels. | mussel specimens (mytilus galloprovincialis) collected from two different areas of the adriatic sea were analysed for diarrhoetic shellfish poisoning (dsp) toxin by three methods: mouse bioassay, the dsp check enzyme immunoassay kit, and high-performance liquid chromatography (hplc). the results obtained confirm that yasumoto's mouse bioassay, capable of detecting all the components of the dsp group, is still necessary to determine the wholesomeness of the product. the elisa method has not alway ... | 1994 | 7886696 |
| effect of lyophilization on the stability of gonyautoxins obtained from contaminated mussels. | this study describes the stability of gonyautoxins (gtx) and c toxins obtained from contaminated mussels and stored at different temperatures in lyophilized samples. analyses of extracts from mussels contaminated with paralytic shellfish poison (psp) indicated the presence of gonyautoxins as the major component in red tides of the north-west coast of spain. these gtx and c toxins were extracted from contaminated mussels (mytilus galloprovincialis lmk) and partially purified by chromatography on ... | 1994 | 7940588 |
| the adhesive protein cdna of mytilus galloprovincialis encodes decapeptide repeats but no hexapeptide motif. | a mussel is attached to hard surfaces by its byssus, which consists of a bundle of threads, each with a fibrous collagenous core coated with adhesive proteins. we constructed a cdna library from rna isolated from the foot of the mussel mytilus galloprovincialis sampled in japan. the library was probed with a nucleotide sequence corresponding to a part of the decapeptide repeat motif in the major adhesive protein of the closely related species m. edulis, and a clone including the whole coding reg ... | 1994 | 8043658 |
| [the heavy metal content in the soft tissues and shells of specimens of 3 varieties of the azov and black sea mussel mytilus galloprovincialis]. | in representatives of 15 micropopulations of the black sea mussels related to three varieties of mytilus galloprovincialis var. dilatata, var. acrocyrta, var. frecviens the content of iron, zinc, lead, copper and cadmium in the soft tissues and shells has been determined with the use of atomic adsorption spectrophotometry. it has been found that of importance is the content of industrial wastes in the habitat of the micropopulation examined rather than its attribution to some or other variety. i ... | 1993 | 8266565 |
| identification of rii-binding proteins in the mollusc mytilus galloprovincialis. | several proteins with m(r) > 70 kda from various tissues of the sea mussel mytilus galloprovincialis were specifically recognized in vitro by the regulatory subunit (type rii alpha) of camp-dependent protein kinase (capk) from porcine heart. however, none of these proteins interacted with the regulatory subunit of capk from the mollusc itself. the results suggest that, unlike mammalian rii, regulatory subunit from mussel lacks the specific residues responsible for interaction with r-binding prot ... | 1996 | 8612771 |
| uptake of mercury species by transplanted mussels mytilus galloprovincialis under estuarine conditions (krka river estuary). | biometric features and physico-chemical conditions are responsible for many of the variables for metal concentrations in indigenous populations of mussels. in order to reduce variations and promote the utility of mussels as bioindicator organisms for environmental mercury concentration levels, individuals of approximately uniform biometric characteristics, selected from a culture of 1-year-old mussels (mytilus galloprovincialis, lmk., were transplanted to four locations in the krka river estuary ... | 1996 | 8643953 |
| cloning, sequencing and sites of expression of genes for the hydroxyarginine-containing adhesive-plaque protein of the mussel mytilus galloprovincialis. | a segment of mytilus galloprovincialis foot protein 3 (mgfp-3) cdna was amplified by means of reverse-transcription (rt)/pcr with degenerate primers. the 5' and 3' regions of the cloned segment were amplified by means of rapid amplification of cdna ends. the 5'-region clones had almost identical nucleotide sequences, but two sequences were found among 3'-region clones. the mgfp-3 coding region was amplified between a 5' untranslated sequence and one of two 3' untranslated sequences. two cdna clo ... | 1996 | 8706704 |
| characterization and immunocytochemical localization of actin and fibronectin in haemocytes of the mussel mytilus galloprovincialis. | cell-extracellular matrix interactions are recognized to be important for human leucocyte functions, including chemotaxis and phagocytosis. these activities depend on a reorganization of the microfilament actin (f-actin) promoted by fibronectin, one of the major components of extracellular matrices. although invertebrate haemocytes are, in many aspects, similar to the human granulocyte-monocyte-macrophage cell lineage, actin and fibronectin have not been well studied in these cells. consequently ... | 1996 | 8737294 |
| influence of the extraction procedure on recovery of okadaic acid from experimentally contaminated mussels. | hepatopancreas samples from mussels (mytilus galloprovincialis) experimentally contaminated with okadaic acid were analysed with yasumoto's mouse bioassay and hplc. a likely effect of some components of the hepatopancreas on the results (matrix effect) was evaluated, and a possible loss of toxin during the extraction phase was quantified. experiments were conducted by comparing two different extraction procedures. under our experimental conditions, the results obtained from mouse bioassay showed ... | 1995 | 8744990 |
| detection of diarrhoetic shellfish toxins in mussels from italy by ionspray liquid chromatography-mass spectrometry. | direct detection of okadaic acid (oa), dinophysistoxin-1 (dtx-1) and some of their related compounds in toxic mussels (mytilus galloprovincialis) is reported using ionspray liquid chromatography-mass spectrometry (lc-isp-ms). this was employed to analyse diarrhoetic shellfish poisoning (dsp) toxins in mussels collected from coastal areas of the northern and southern adriatic sea. dtx-1 was found in some samples from both the northern and southern adriatic and this is the first report of the unam ... | 1995 | 8866617 |
| a member of the arthropod defensin family from edible mediterranean mussels (mytilus galloprovincialis) | plasma from the mussel mytilus galloprovincialis previously immunized by injecting them with bacteria contains several bactericidal proteins. one protein, mgd-1, was purified by reverse-phase hplc of supernatant from acidified cell-free hemolymph. its biological activity is directed against both gram-positive and gram-negative bacteria but it is not cytotoxic towards human erythrocytes nor protozoa. as determined by mass spectrometry, the molecular mass of mgd-1 is 4418 da. primary-structure ana ... | 1996 | 8925841 |
| a rapid tissue culture assay for the detection of okadaic acid and related compounds in mussels. | okadaic acid, the main toxin of the diarrhoetic shellfish poisoning (dsp) group, shows high cytotoxicity to buffalo green monkey (bgm) kidney cell cultures after different times according to concentration. a rapid method of detecting the presence of okadaic acid and related compounds in contaminated mussels by means of a cytotoxicity test is presented. the method is based on direct microscopic observation of toxin-induced morphological changes in bgm cell cultures. a high correlation (r = 0.950; ... | 1997 | 9080579 |
| use of acetylcholinesterase in perna perna and mytilus galloprovincialis as a biomarker of pollution in agadir marine bay (south of morocco). | | 1997 | 9136652 |
| intron-length polymorphism at the actin gene locus mac-1: a genetic marker for population studies in the marine mussels mytilus galloprovincialis lmk. and m. edulis l. | a novel intron-length polymorphism at the actin gene locus mac-1 is here reported and used as a genetic marker for population studies in mussels of the genus mytilus. two closely related genes subsequently identified as alleles, mac-1a1 and mac-1b1, from a genomic library of m. galloprovincialis were partially cloned and sequenced. they mainly differed from each other by a 65-bp insertion within their first intron. polymerase chain reaction (pcr) primers were designed outside the insertion. the ... | 1997 | 9200839 |
| structure and differential target sensitivity of the stimulable cytotoxic complex from hemolymph of the mediterranean mussel mytilus galloprovincialis. | a cytotoxic protein complex of 320 kda was isolated from dialyzed plasma of the edible mussel, mytilus galloprovincialis. constituted by the assembly of several different proteins, the complex exhibits selective killing against eukaryotic cells, including erythrocytes, mouse tumor cells and protozoan parasites. high variability, which was not correlated with protein concentration, suggested that the immune response of naive mussels was in various stages of activation. stimulation assays by diffe ... | 1997 | 9247087 |
| peroxidase-release associated with phagocytosis in mytilus galloprovincialis haemocytes. | fluorescence spectra of the supernatant of adherent haemocyte monolayers from mytilus galloprovincialis, supplemented with homovanillic acid or with a tyrosyl peptide glycylglycyltyrosine (ggy), were recorded before and after stimulation by zymosan. the formation of fluorescent derivatives was observed to have spectral characteristics similar to those of fluorescent compounds generated by the exposure of homovanillic acid or ggy to a horseradish peroxidase/hydrogen peroxide system in vitro. luci ... | 1997 | 9258608 |
| identification of protein kinase c isoforms in marine mussels. | in this paper we have examined the distribution of some isoforms of protein kinase c in different tissues from the sea mussel mytilus galloprovincialis lmk.. by immunoblot analysis, we have detected the presence of at least three pkc isoforms, all preferably associated with the cellular cytosolic fraction. the ca(2+)-independent form pkc delta was separated from the ca(2+)-dependent forms (pkc alpha and beta) by means of an ionic change chromatography on de-52. a comparative study was carried ou ... | 1997 | 9305542 |
| male-predominant carboxylesterase expression in the reproductive system of molluscs and insects: immunochemical and biochemical similarity between mytilus male associated polypeptide (map) and drosophila sex-specific esterase s. | we suggested that sexual differentiation of the reproductive system in gonochoric species of invertebrates can be characterized by common molecular mechanisms in spite of high morphological divergences of reproductive tract organs in different animal groups. the present study focused on this problem and report our observations on biochemical characteristics of male-associated polypeptide (map) identified in the gonad tissue of bivalve molluscs, mytilus galloprovincialis, in comparison to those o ... | 1997 | 9418010 |
| induction of peroxisomal oxidases in mussels: comparison of effects of lubricant oil and benzo(a)pyrene with two typical peroxisome proliferators on peroxisome structure and function in mytilus galloprovincialis. | marine mussels are used as bioindicators of water pollution in marine and estuarine environments in the so-called "mussel watch" programs because of their capacity to accumulate numerous organic xenobiotics including aromatic hydrocarbons. in this study, we have analyzed the effects of two xenobiotics [benzo(a)pyrene and the water accommodated fraction of a lubricant oil] and two typical (rodent) peroxisome proliferators (clofibrate and dioctyl phthalate) on structure and function of peroxisomes ... | 1998 | 9512728 |
| tissue dose, dna adducts, oxidative dna damage and cyp1a-immunopositive proteins in mussels exposed to waterborne benzo[a]pyrene. | a collaborative study was performed on mediterranean mussels (mytilus galloprovincialis) exposed to a wide dose-range (0.5-1000 ppb) of benzo[a]pyrene (b[a]p). we selected this model polycyclic aromatic hydrocarbon in order to confirm the formation of a specific dna adduct, previously detected in gill dna, and to clarify the in vivo effects of this mutagenic chemical requiring host-metabolism in mussels. b[a]p concentration reached consistently higher values in the digestive gland than in other ... | 1998 | 9635486 |
| prevalence of mytilicola intestinalis (copepoda: mytilicolidae) and urastoma cyprinae (turbellaria: hypotrichinidae) in marketable mussels mytilus galloprovincialis in italy. | marketable mussels mytilus galloprovincialis traded with commercial certification from production sites in italy and abroad (france, spain) were examined for the presence of mytilicola intestinalis (copepoda: mytilicolidae) and urastoma cyprinae (turbellaria: hypotrichinidae) from october 1994 to february 1996. the prevalence of m. intestinalis was 4.1% and 4.7% respectively in mussels from lerici (la spezia) and s. pietro in volta (venice), whereas it rose to 57.9% in the samples from spain. m. ... | 1998 | 9676254 |
| the turbellarian urastoma cyprinae from edible mussels mytilus galloprovincialis and mytilus californianus in baja california, nw mexico | the turbellarian urastoma cyprinae (graff, 1913) was found in the mantle cavity of mytilus galloprovincialis lamarck and mytilus californianus conrad, on the pacific coast of baja california nw mexico. this is the first record of this turbellarian for bivalves from the pacific coast of north america. in m. galloprovincialis from an exposed rocky shore, prevalence ranged from 10 to 87% and mean number of turbellarians per infested mussel was 1.9; in a culture area prevalence ranged from 57 to 100 ... | 1998 | 9784343 |
| seasonal variation and effects of age, food availability, size, gonadal development, and parasitism on the hemogram of mytilus galloprovincialis | the study of hemogram characteristics in a population of cultured mussels through a period of 18 months revealed that the concentration of circulating mytilus galloprovincialis hemocytes varied, following a seasonal pattern, being positively correlated with water temperature. most mussels had between 1 and 5 x 10(6) hemocytes/ml of hemolymph. differential counts showed that m. galloprovincialis hemolymph contained more granulocytes than hyalinocytes. the hemolymph cell concentration was not asso ... | 1998 | 9784355 |
| immunochemical investigations of cytochrome p450 forms/epitopes (cyp1a, 2b, 2e, 3a and 4a) in digestive gland of mytilus sp. | western blot analysis of microsomes and partially purified cytochrome p450 (cyp) from digestive gland of mytilus edulis was carried out using polyclonal antibodies to hepatic perca fluviatilis cyp1a, oncorhynchus mykiss cyp3a and rat cyp2b, cyp2e and cyp4a isoforms. multiple cyp bands were detected in partially purified cyp compared to single bands for microsomes for anti-cyp1a, anti-cyp2b, anti-cyp2e and anti-cyp3a. in contrast, anti-cyp4a showed two distinct bands for both. the apparent molecu ... | 1998 | 9972478 |
| canning process that diminishes paralytic shellfish poison in naturally contaminated mussels (mytilus galloprovincialis). | changes in toxin profile and total toxicity levels of paralytic shellfish poison (psp)-containing mussels were monitored during the standard canning process of pickled mussels and mussels in brine using mouse bioassays and high-performance liquid chromatography. detoxification percentages for canned mussel meat exceeded 50% of initial toxicity. total toxicity reduction did not fully correspond to toxin destruction, which was due to the loss of psp to cooking water and packing media of the canned ... | 1999 | 10340673 |
| high levels of yessotoxin in mussels and presence of yessotoxin and homoyessotoxin in dinoflagellates of the adriatic sea. | identification of ytx and homoytx in natural phytoplankton populations containing significant amounts of gonyaulax polyedra and determination of detailed toxin profiles of mussels (mytilus galloprovincialis) periodically collected from two sites of the northern adriatic coast from february to october 1997 was performed by lc-fld following derivatization with adam or dmeq-tad and lc-ms and lc-ms-ms. oa and ytx concentrations were recorded in the range 0.11-2.31 and 0.18-9.02 microg per g of hepat ... | 1999 | 10400302 |
| long-term survival of cryptosporidium parvum oocysts in seawater and in experimentally infected mussels (mytilus galloprovincialis). | transmission of infectious oocysts of cryptosporidium parvum via surface- and drinking-water supplies has been reported and many surface waters flow into the sea, potentially causing runoff of animal-infected faeces. eating raw mussels is a common practice in many countries, increasing the public's risk of acquiring enteric pathogens. the aims of the present study were to estimate how long c. parvum oocysts remain infectious in artificial seawater, to determine if the oocysts are retained in mus ... | 1999 | 10404265 |
| occurrence of vibrio and other pathogenic bacteria in mytilus galloprovincialis (mussels) harvested from adriatic sea, italy. | sixty-two samples of mytilus galloprovincialis (mussels) harvested from approved shellfish waters in the adriatic sea were examined for the presence of vibrio, salmonella, campylobacter, and verocytotoxin producing escherichia coli. vibrio spp. were isolated from 48.4% of samples; the species most frequently found were v. alginolyticus (32.2%) and v. vulnificus (17.7%), followed by v. cincinnatiensis (3.2%), v. parahaemolyticus (1.6%), v. fluvialis (1.6%) and v. cholerae non-o1 (1.6%). v. paraha ... | 1999 | 10477069 |
| dynamics of microcystins in the mussel mytilus galloprovincialis. | the accumulation and depuration of hepatotoxins produced by the freshwater cyanobacterium microcystis aeruginosa in the mussel mytilus galloprovincialis was studied. mussels were fed daily 10(5) cells/ml of the toxic cyanobacterium that produces microcystin-lr (mcyst-lr), for four days. after that period animals were placed in toxin free water and were fed the diatom nitzschia sp. during two weeks the concentration of the toxin in the mussels, as also in their feces and in the water where animal ... | 1999 | 10484739 |
| myticin, a novel cysteine-rich antimicrobial peptide isolated from haemocytes and plasma of the mussel mytilus galloprovincialis. | we report here the isolation of two isoforms of a novel cysteine-rich peptide from haemocytes (isoform a of 4.438 da and b of 4.562 da) and plasma (isoform a) of the mussel, mytilus galloprovincialis. the two molecules display antibacterial activity against gram-positive bacteria, whereas only isoform b is active against the fungus fusarium oxysporum and a gram-negative bacteria escherichia coli d31. complete peptide sequences were determined by a combination of edman degradation, mass spectrome ... | 1999 | 10491159 |
| mussel defensins are synthesised and processed in granulocytes then released into the plasma after bacterial challenge. | mgd1 (mytilus galloprovincialis defensin 1), a new member of the arthropod defensin family, is a 4 kda antibacterial peptide previously isolated from the plasma of mediterranean mussels. we report here the presence of mgd1 in the organelle-rich fraction of hemocytes and the cdna sequence corresponding to mgd1 and one new isoform mrna: mgd2. sequence analysis indicated that mgds are synthesised as precursors consisting of a putative signal peptide of 21 residues, the active peptide of 39 amino ac ... | 1999 | 10564642 |
| long-term variations of man-made radionuclide concentrations in a bio-indicator mytilus galloprovincialis from the french mediterranean coast. | results from a 14-year monitoring (1984-1997) of man-made radionuclide (137cs and 106ru) levels in mytilus galloprovincialis collected monthly on the french mediterranean coast are presented. in this area sources of man-made radionuclides are on the one hand atmospheric fallout from both the past nuclear testings and the chernobyl accident and on the other hand discharges from nuclear installations located on the rhône river banks, especially those from the spent nuclear fuel reprocessing plant ... | 1999 | 10568268 |
| detection of casein fragments in an invertebrate and in a vertebrate using in situ hybridization. | using a non-radioactive in situ hybridization procedure it has been demonstrated that both invertebrates such as the mollusc mytilus galloprovincialis and lower vertebrates such as the fish cyprinus carpio express bovine alphas1- and kappa-casein homologous mrnas. in particular, positive results were found in molluscan immunocytes, and in cells located in different fish tissues: intestine, endocrine pancreas and kidney. these findings suggest that the casein genes are highly conserved throughout ... | 1999 | 10573188 |
| seasonal variation of xanthine oxidoreductase activity in the digestive gland cells of the mussel mytilus galloprovincialis: a biochemical, histochemical and immunochemical study. | the activity and the tissue distribution of the oxygen radical producing enzyme xanthine oxidoreductase (xor) were measured in the digestive gland of the common marine mussel mytilus galloprovincialis lmk along an annual cycle. no xanthine oxidase (xox) activity could be measured, the enzyme only displaying xanthine dehydrogenase (xdh) activity in all the cases. this is interpreted as a mechanism to avoid the harmful effects of the oxygen radicals that would be produced by xox during periods fol ... | 1999 | 10629940 |
| immunochemical localisation of proliferating cells in mussel digestive gland tissue. | the distribution of proliferating cells in the digestive gland of the common marine mussel, mytilus galloprovincialis lmk, was investigated by means of immunochemical techniques employing pc10, a commercial monoclonal antibody to the proliferating cell nuclear antigen (pcna). immunoblot analysis of digestive gland whole homogenates revealed a single crossreactive band of 36-37 kda, identical to the corresponding protein of rat liver and murine melanoma cells. a band of slightly higher electropho ... | 1999 | 10661321 |
| peroxisomes in molluscs, characterization by subcellular fractionation combined with western blotting, immunohistochemistry, and immunocytochemistry. | peroxisomes of the digestive glands of mussels, mytilus galloprovincialis lmk, were investigated by immunoblotting and immunohistochemistry using rabbit antibodies against several mammalian hepatic peroxisomal proteins. western blot analysis of main subcellular fractions revealed immunoreactive polypeptides with molecular weights comparable to those of the corresponding mammalian hepatic proteins. they could be localized to the peroxisomal matrix in the case of catalase, multifunctional enzyme ( ... | 2000 | 10664070 |
| heavy metals in mussels (mytilus galloprovincialis) from the lonian sea, italy. | concentrations of six heavy metals (hg, pb, cd, cr, zn, and sn) were determined in mussels (mytilus galloprovincialis) collected between june and september 1997 from 10 locations along a sound formed by two inlets (mar piccolo) near the gulf of taranto (ionian sea, italy). the average concentrations of the heavy metals found in mussels samples were 0.15 mg/kg for hg, 1.19 mg/kg for pb, 0.64 mg/kg for cd, 0.31 mg/kg for cr, 5.15 mg/kg for zn, and 0.54 mg/kg for sn. the concentrations of heavy met ... | 2000 | 10678436 |
| immunocytochemical localization of a urate oxidase immunoreactive protein in the plasma membranes and membranes of the secretory/endocytic compartments of digestive gland cells of the mussel mytilus galloprovincialis. | the subcellular compartmentalization of urate oxidase (uox) in the digestive glands of mussels, mytilus galloprovincialis lmk, was studied by means of immunoblotting and immunocytochemistry, using an antibody raised in rabbit against rat liver uox. western blot analysis of subcellular fractions revealed an immunoreactive polypeptide with a molecular weight similar to the corresponding mammalian hepatic protein. this crossreactive polypeptide of 32 kda was particle-bound yet not peroxisome-associ ... | 2000 | 10711424 |
| detection of infectious cryptosporidium parvum oocysts in mussels (mytilus galloprovincialis) and cockles (cerastoderma edule). | infective cryptosporidium parvum oocysts were detected in mussels (mytilus galloprovincialis) and cockles (cerastoderma edule) from a shellfish-producing region (gallaecia, northwest spain, bounded by the atlantic ocean) that accounts for the majority of european shellfish production. shellfish were collected from bay sites with different degrees of organic pollution. shellfish harboring c. parvum oocysts were recovered only from areas located near the mouths of rivers with a high density of gra ... | 2000 | 10788352 |
| immunomodulation by recombinant human interleukin-8 and its signal transduction pathways in invertebrate hemocytes. | we report the presence of interleukin (il)-8-immunoreactive molecules in hemocytes from the mollusc mytilus galloprovincialis. functional studies demonstrate that recombinant human (rh)il-8 provokes conformational changes, induces chemotaxis, and increases bacterial phagocytic activity in hemocytes. rhil-8 induces cell shape changes via protein kinase a and c pathways. these morphological changes are followed by reorganization of the actin microfilaments. the findings suggest that, as previously ... | 2000 | 10823250 |
| enzymatic biomarker measurement and study of dna adduct formation in benzo | the aim of this study was to improve the knowledge on the metabolic pathways involved in benzo[a]pyrene (b[a]p) activation and on the relationship between adduct levels and enzymatic biomarker activities. with this purpose, a model to assess pollutant exposure via food supply has been developed for the sentinel organism, mytilus galloprovincialis. mussels were fed for 4 weeks with b[a]p-contaminated feed (50 mg/kg dry weight mussel). bioaccumulation was studied by determination of b[a]p concentr ... | 2000 | 10854671 |
| differential distribution and defence involvement of antimicrobial peptides in mussel. | in previous papers, we characterised 3 types of 4-kda, cysteine-rich, cationic antimicrobial peptides: mgds (for mytilus galloprovincialis defensins), mytilins and myticins, which are abundant in the mussel hemocytes. in the present work, we revealed a differential distribution of the cells expressing the different genes. in addition, using confocal and electron microscopy, we confirmed that defensins and mytilins were partially located in different sub-types of circulating hemocytes although th ... | 2000 | 10893191 |
| detection of cryptosporidium oocysts in bivalve molluscs destined for human consumption. | clams (dosinia exoleta, ruditapes philippinarum, venerupis pullastra, venerupis rhomboideus, venus verrucosa), mussels (mytilus galloprovincialis), and oysters (ostrea edulis) were tested for the presence of cryptosporidium sp. oocysts using various stain techniques and a commercially available kit containing fluorescein isothiocyanate-conjugated monoclonal antibodies. all molluscs were harvested in northwest spain (galicia) except for r. philippinarum, which was from italy, and 1 of the 6 oyste ... | 2000 | 10958471 |
| in vitro production of superoxide and nitric oxide (as nitrite and nitrate) by mytilus galloprovincialis haemocytes upon incubation with pma or laminarin or during yeast phagocytosis. | the phagocytic process is one of the most important elements of the self-defence system in mammals as well as in molluscs. in mammalian phagocytes, superoxide participates in the innate defence system by combining with nitric oxide to generate peroxynitrite, a strong oxidant that possesses highly cytotoxic properties against bacteria. to evidence a role of nitric oxide in the self-defence system of the marine bivalve mytilus galloprovincialis similar to the role observed in the mammalian defence ... | 2000 | 10961451 |
| frog lim-1-like protein is expressed predominantly in the nervous tissue, gonads, and early embryos of the bivalve mollusc mytilus galloprovincialis. | in a few well-known cases, the biological consequences of the disruption of lim-1 homeodomain (hd) genes have demonstrated the important roles of these genes in vertebrate development, especially in the nervous tissue, kidney, and gonads. functional assay approaches require information not only about lim-1 gene organization, but also about properties and tissue localization of lim-1 proteins. although lim-1 genes have been identified in certain phyla of invertebrates, no information is available ... | 2000 | 10975640 |
| detection of localized methylmercury contamination by use of the mussel adductor muscle in minamata bay and kagoshima bay, japan. | based on our previous finding that the concentrations of total mercury in mussel adductor muscle approximated those of methylmercury, we compared concentrations of total mercury in the adductor muscle of the mussel mytilus galloprovincialis, collected from four sites around minamata city from 1993 to 1995 and four sites in kagoshima bay from 1997 to 1998, to assess the level of localized methylmercury contamination. though the input of mercury from the chemical plant had stopped by around 1970, ... | 2000 | 11036979 |
| modulation of the chemiluminescence response of mediterranean mussel (mytilus galloprovincialis) haemocytes. | the influence of several factors on the chemiluminescence (cl) activity of haemocytes from the mediterranean mussel (mytilus galloprovincialis) was studied. haemocytes were stimulated in vitro with different concentrations of zymosan, phorbol 12-myristate 13-acetate (pma) and lipopolysaccharide (lps) (adding superoxide dismutase, sod, to the zymosan-stimulated haemocytes in order to test the specificity of the reaction). the in vitro effect of the clam pathogens vibrio tapetis (bacteria) and a p ... | 2000 | 11081438 |
| surface interactions between escherichia coli and hemocytes of the mediterranean mussel mytilus galloprovincialis lam. leading to efficient bacterial clearance. | the role of type 1 fimbriae in the interactions between escherichia coli and mytilus galloprovincialis lam. hemocytes was evaluated. the association of fimbriated strain mg155 with hemocyte monolayers at 18 degrees c was 1.5- and 3- to 4-fold greater than the association of unfimbriated mutant aaec072 in artificial seawater and in hemolymph serum, respectively. such differences were apparently due to different adhesive properties since mg155 adhered more efficiently than aaec072 when hemocytes w ... | 2001 | 11133482 |
| comparison of dinophysistoxin-1 and esterified dinophysistoxin-1 (dinophysistoxin-3) contents in the scallop patinopecten yessoensis and the mussel mytilus galloprovincialis. | okadaic acid (oa) homologues in toxic dinoflagellate dinophysis fortii, scallops patinopecten yessoensis and mussels mytilus galloprovincialis collected at the same site in mutsu bay, japan were determined by high-performance liquid chromatography fluorescence detection (hplc-fld) as their 9-anthryldiazomethane (adam) derivatives. prominent toxin in the scallops and the mussels was esterified dinophysistoxin-1 (dtx3) and dinophysistoxin-1 (dtx1), respectively, although only dtx1 was detected in ... | 2001 | 11137552 |
| the effect of mussel size, temperature, seston volume, food quality and volume-specific toxin concentration on the uptake rate of psp toxins by mussels (mytilus galloprovincialis lmk). | the accumulation of paralytic shellfish poisoning (psp) toxins by bivalves is a serious threat to public health all over the world. however, very little is known about the uptake kinetics of these toxins and the environmental factors that may modify this process. we have studied the effect of mussel size, temperature, seston volume, food quality, and volume-specific toxin concentration (vostoc), on the uptake rate of paralytic shellfish poisoning (psp) toxins by mussels (mytilus galloprovinciali ... | 2001 | 11165303 |
| in vitro production of peroxynitrite by haemocytes from marine bivalves: c-elisa determination of 3-nitrotyrosine level in plasma proteins from mytilus galloprovincialis and crassostrea gigas. | peroxynitrite is increasingly proposed as a contributor to defence system in marine bivalve. it can be formed by combination of superoxide and nitric oxide, and can react with tyrosine residues of proteins giving rise to 3-nitrotyrosine. | 2001 | 11231884 |
| the integrated use of chemical analysis, cytochrome p450 and stress proteins in mussels to assess pollution along the galician coast (nw spain). | aliphatic and polycyclic aromatic hydrocarbons (pahs) as well as biochemical markers, such as cytochrome p450, benzo(a)pyrene hydroxylase (bph) and stress-70 proteins, were determined in mussels (mytilus galloprovincialis) collected in october 1995 from several locations along the galician coast (nw spain). the survey proved the existence of a pollution gradient among sampling sites and a chronic exposure to petrogenic and pyrolitic hydrocarbons. this was reflected in mussel tissue levels of pah ... | 2001 | 11234544 |
| biochemical response of the mussel mytilus galloprovincialis from bizerta (tunisia) to chemical pollutant exposure. | three biomarkers (glutathione s-transferase [gst] activity, catalase [cat] activity, and malonedialdehyde [mda] levels) were measured in specimens of the mussel mytilus galloprovincialis collected from two different stations (bc and mj) in the lagoon of bizerta (tunisia). animals were allowed to acclimate in the laboratory for some days. they were then exposed for 48 h to two concentrations of pp'dde and two doses of mercury chloride. the acclimation period increased cat activities and mda level ... | 2001 | 11243324 |
| biochemical properties of metalloproteinases from the hemolymph of the mussel mytilus galloprovincialis lam. | the expression of matrix metalloproteinases (mmp) with gelatinase activity was found in the whole hemolymph of the marine mussel mytilus galloprovincialis lam. cleavage activity was specific for gelatin; very little activity towards human type-iv collagen, and no activity for cold fish gelatin, casein or bovine serum albumin were detected. edta and 1,10-phenanthroline were inhibitory, suggesting that mussel mmps require divalent cations for their proteolytic activity; in fact, the presence of ex ... | 2001 | 11250546 |
| polycyclic aromatic hydrocarbons in mussels (mytilus galloprovincialis) from the ionian sea, italy. | concentrations of eight polycyclic aromatic hydrocarbons (pahs) (phenanthrene, anthracene, fluoranthene, pyrene, benz[a]anthracene, benzo[a]pyrene, dibenz[a,h]anthracene, benzo[ghi]perylene) were determined in mussels (mytilus galloprovincialis) collected between june and september 1995 from 10 locations along a sound of sea formed by two inlets (mar piccolo) close to the gulf of taranto (ionian sea, italy). in mussels the concentrations of total pahs were between 14.8 and 645.3 microg/kg wet we ... | 2001 | 11252489 |
| dna integrity and total oxyradical scavenging capacity in the mediterranean mussel, mytilus galloprovincialis: a field study in a highly eutrophicated coastal lagoon. | in mediterranean coastal lagoons, the combination of human impact and wide variability of natural environmental factors can lead to upsets in ecosystem homeostasis resulting in biodiversity decline. oxidative damage has been causally linked to various kinds of environmental stress, both natural and artificial, the result being impairment of cellular functions. dna damage and the efficiency of antioxidant defences in mytilus galloprovincialis from the highly eutrophicated orbetello lagoon (tuscan ... | 2001 | 11254944 |
| ultrastructural characterisation of marteilia species (paramyxea) from ostrea edulis, mytilus edulis and mytilus galloprovincialis in europe. | a focused ultrastructural study of marteilia spp. found in cultured ostrea edulis, mytilus edulis and mytilus galloprovincialis from france and spain was conducted with emphasis placed on haplosporosomes, striated plate-like inclusions and spore wall morphology. two types of haplosporosome were identified, sphaeroid and oblate, which were common to the parasite in all 3 host species. a total of 492 haplosporosomes were measured; those from the marteilia sp. in mytilus spp. were marginally smalle ... | 2001 | 11324815 |
| first record of marteilia sp. in mussels mytilus galloprovincialis in croatia. | marteiliosis is a disease of molluscs caused by marteilia refringens in europe and m. sydneyi in australia. during routine examination of cultured mussels mytilus galloprovinciallis in the northern adriatic, the occurrence of marteilia sp. was recorded with a prevalence of 5%. this parasite was not detected in flat oysters reared in the same area. the affiliation of the detected parasite in m. galloprovinciallis was confirmed by in situ hybridization using a m. refringens probe, specific at the ... | 2001 | 11324816 |
| closed-circuit system for the depuration of mussels experimentally contaminated with hepatitis a virus. | in italy, the consumption of raw or slightly cooked mussels represents the most important risk factor for the transmission of hepatitis a virus (hav). although there exist effective methods for the bacterial depuration of contaminated mussels, these methods are poorly effective on enteric viruses. the objective of the present study was to evaluate the effectiveness of a closed-circuit depuration system that uses both ozone and uv light for disinfecting water and that allows salinity and temperat ... | 2001 | 11403143 |
| direct evidence for homologous recombination in mussel (mytilus galloprovincialis) mitochondrial dna. | the assumption that animal mitochondrial dna (mtdna) does not undergo homologous recombination is based on indirect evidence, yet it has had an important influence on our understanding of mtdna repair and mutation accumulation (and thus mitochondrial disease and aging) and on biohistorical inferences made from population data. recently, several studies have suggested recombination in primate mtdna on the basis of patterns of frequency distribution and linkage associations of mtdna mutations in h ... | 2001 | 11420358 |
| izmit bay (turkey) ecosystem after marmara earthquake and subsequent refinery fire: the long-term data. | as a part of marmara sea, izmit bay (turkey) has been one of the most polluted sites in the region for the last 25 years. on 17 august 1999, a powerful earthquake along the north anatolian fault struck the eastern part of the marmara region including izmit bay. the earthquake destroyed many coastal cities. the bay was also affected by the quake and subsequent fire in the refinery situated on the north-eastern coast of the bay. oceanographic characteristics and polycyclic aromatic hydrocarbon (pa ... | 2001 | 11436816 |
| biomarkers and trace metals in the digestive gland of indigenous and transplanted mussels, mytilus galloprovincialis, in venice lagoon, italy. | the aim of this study was to investigate the cellular and biochemical response of mussels, mytilus galloprovincialis, transplanted from a relatively pristine site to a polluted one and vice versa in the lagoon of venice (northeast of italy) and to apply auto-metallography, a rapid and sensitive histochemical technique, to determine the bio-available fraction of heavy metals accumulated in the body tissues of organisms. animal digestive glands have been used for morphological analyses (lysosomal ... | 2000 | 11460729 |
| the use of acetylcholinesterase activity in ruditapes decussatus and mytilus galloprovincialis in the biomonitoring of bizerta lagoon. | the variations of acetylcholinesterase activity, considered to be a biochemical biomarker of organophosphorus and carbamate compounds, was followed in mediterranean clams (ruditapes decussatus) and mussels (mytilus galloprovincialis) as a function of season and sampling sites in the lagoon of bizerta (tunisia). results show that acetylcholinesterase activity in clams collected from station a was the lowest and was so throughout the year. this low activity may be due to the input of non-treated w ... | 2001 | 11574292 |
| experimental studies on the biokinetics of 134cs and 24am in mussels (mytilus galloprovincialis). | the biokinetics of 134cs and 241am in mussel species contaminated through water pathway has been studied under laboratory conditions. at equilibrium, the concentration factors for 134cs and 241am in small and large mussels were 2.80 and 2.57 and 200 and 150, respectively. the concentration factor of 134cs in soft parts of the mussels was significantly high than whole body and shell tissue. however, the concentration factors of 241am in soft parts and shell tissue samples were found to have simil ... | 2001 | 11686636 |
| polycyclic aromatic hydrocarbon pollution in native and caged mussels. | during 1999, a biological monitoring study was conducted at four sites along the ligurian coast (cornigliano, voltri, vado ligure and sanremo). at each site the concentration and composition of polycyclic aromatic hydrocarbons (pah) were investigated in native and caged mussels. the mussels (mytilus galloprovincialis), sampled in the spring and the autumn, showed different accumulation patterns according to the source of pollution they were exposed to. the pah concentrations were higher in the n ... | 2001 | 11693650 |
| structural study of a possible neoplasia detected in mytilus galloprovincialis collected from the ria of vigo (nw spain). | several specimens of mytilus galloprovincialis, collected in the ria of vigo over a non-consecutive 2 yr period (1993 to 1994 and 1996 to 1997), presented a possible gonadal neoplasm, entailing morphologically abnormal germinal cells distributed throughout the follicle and invading the adjacent storage tissue. in some cases, affected cells were noted in gonoducts and in haemic sinusoids. prevalence of this anomaly in the samples was 6%, and all affected individuals were found between april and j ... | 2001 | 11797918 |
| lysosomal membrane stability and metallothioneins in digestive gland of mussels (mytilus galloprovincialis lam.) as biomarkers in a field study. | the lysosomal membrane destabilization and the metallothionein content in the digestive gland cells of mussels (mytilus galloprovincialis lam.), collected along the east coast of the north adriatic (istrian and kvarnerine coast, croatia), were examined over a period of four years (1996-1999). the lysosomal membrane stability, as a biomarker of general stress, showed that the membrane labilization period in mussels from polluted, urban- and industrial-related areas was significantly decreased (p ... | 2001 | 11827125 |
| effects of depuration of molluscs experimentally contaminated with escherichia coli, vibrio cholerae 01 and vibrio parahaemolyticus. | the aim of the present study was to investigate the behaviour of two pathogenic vibrios (vibrio cholerae o1 and vibrio parahaemolyticus) during depuration and to compare it with that of escherichia coli, used as an indicator of suitability for consumption. | 2002 | 11872121 |
| signaling pathways involved in the physiological response of mussel hemocytes to bacterial challenge: the role of stress-activated p38 map kinases. | in this work the mechanisms of transduction triggered in mytilus galloprovincialis hemocytes by bacterial challenge were investigated in an in vitro model of infection of hemocyte monolayers with escherichia coli. western blot analyses of hemocyte extracts with phospho-specific anti-mapk (mitogen activated protein kinase) antibodies indicate that e. coli induced a time dependent activation of different classes of mapks, mainly of the stress-activated p38 mapk. p38 activation was confirmed by the ... | 2002 | 11888647 |
| dna damage and apoptosis in the mussel mytilus galloprovincialis. | the effects of known genotoxic substances (4-nitroquinoline-n-oxide, benzo[a]pyrene, teniposide, etoposide, cycloheximide, tributyltin) on human cells (flc, hl-60) and on mussels were investigated. the correlations between formation of dna strand breaks and dna fragmentation characteristic for the process of apoptosis were estimated. strand breaks induced by 4-nitroquinoline-n-oxide and benzo[a]pyrene did not correlate with dna fragmentation detected in the process of apoptosis. induction of int ... | 2002 | 11939292 |
| temporal trends of cd, cu, hg, pb and zn in mussel (mytilus galloprovincialis) from the spanish north-atlantic coast 1991-1999. | temporal trends for heavy metals (cd, cu, hg, pb and zn) in mussel (mytilus galloprovincialis) from the galician and cantabrian areas in spain, where samples were yearly collected from 1991 to 1999, are presented. this study was carried out by the centro oceanográfico de vigo of the instituto español de oceanografia (i.e.o.) as part of the spanish contribution to the joint assessment and monitoring programme (jamp) of the ospar convention. the experimental work and subsequent statistical treatme ... | 2002 | 11991527 |
| toxicity of methyl tert-butyl ether to marine organisms: ambient water quality criteria calculation. | in response to increasing concerns over the detection of methyl tert-butyl ether (mtbe) in groundwater and surface water and its potential effects in aquatic ecosystems, industry and the united states environmental protection agency (usepa) began to collaborate in 1997 to develop aquatic toxicity databases sufficient to derive ambient water quality criteria for mtbe consistent with usepa requirements. acute toxicity data for seven marine species, chronic toxicity data for an invertebrate, and pl ... | 2002 | 11996128 |
| production of nitric oxide by mussel (mytilus galloprovincialis) hemocytes and effect of exogenous nitric oxide on phagocytic functions. | in the present study, the ability of mussel (mytilus galloprovincialis) hemocytes to produce nitric oxide (no) in response to phorbol myristate acetate (pma) was determined using the griess reaction. significant no production was found in these cells in response to pma. this stimulation was reversed in the presence of the no synthase inhibitor, n(g)-methyl-l-arginine (l-nmma). moreover, the effect of the pre-incubation of hemocytes with no was also determined on phagocytic immune functions of mu ... | 2002 | 12031469 |
| antioxidant enzymes and peroxisome proliferation in relation to contaminant body burdens of pahs and pcbs in bivalve molluscs, crabs and fish from the urdaibai and plentzia estuaries (bay of biscay). | with the aim of studying levels of antioxidant and peroxisomal enzymes and the structure of peroxisomes in relation to body burdens of polycyclic aromatic hydrocarbons (pahs) and polychlorinated biphenyls (pcbs), mussels mytilus galloprovincialis, oysters crassostrea sp., crabs carcinus maenas and mullets mugil cephalus were sampled in two basque estuaries (bay of biscay): urdaibai (laida, txatxarramendi, arteaga, and downstream a sewage treatment plant-stp) and plentzia. in general, animals sho ... | 2002 | 12062156 |