visualization of nucleolar organizer regions im mammalian chromosomes using silver staining.a simple ammoniacal silver staining procedure, designated ag-as, differentially stains the chromosomal locations of ribosomal dna in certain mammalian species. this was critically demonstrated by ag-as staining of the nucleolus organizer regions in karyotypes of the same species and cell lines used for locating the ribosomal cistrons by dna/rna in situ hybridization. with ag-as, silver stained nors (ag-nors) are visualized as black spherical bodies on yellow-brown chromosome arms. ag-nors were v ...197553131
pathomorphologic findings in short-tailed voles (microtus agrestis) experimentally-infected with frenkelia microti.following oral infection of microtus agrestis with sporocysts of frenkelia microti, transient focal necrosis and cellular infiltrations in the liver, hyperplasia of lymphoid organs, and inflammatory infiltrations in the heart, pulmonary veins, skeletal muscles and brain occurred during the first asexual multiplication period of the parasite in the liver. frenkelia cysts were first observed in the brain 23 days after infection.1979113563
properties of mammalian cells transformed by temperature-sensitive mutants of avian sarcoma virus.fibroblasts from european field vole (microtus agrestis) and from normal rat kidney (nrk) have been infected by avian sarcoma virus mutants which are temperature-sensitive for the maintenance of transformation. these cells are transformed at 33 degrees c, but show normal cell characteristics in morphology, colony formation in agar, saturation density, sugar uptake and membrane proteins at 39 degrees c and 40 degrees c, the nonpermissive temperatures. ts mutant virus was rescued from most of the ...1977195741
quantitation and localization of rous sarcoma virus-specific rna in transformed and revertant field vole cells.hybridization analysis of rna from transformed clones of rous sarcoma virus (rsv)-infected field vole cells and revertant subclones indicated the presence of similar amounts of viral-specific rna in both cell types. employing both a relatively uniform and representative complementary dna probe and genomelength complementary dna, we have demonstrated that the majority of rsv proviral dna is transcribed into viral-specific rna in both transformed and revertant clones. the viral-specific rna is pre ...1978203719
peptide analysis of the transformation-specific antigen from avian sarcoma virus-transformed cells.sera from rabbits bearing tumors induced by avian sarcoma virus (asv) were ussed to immunopecipitate virus-specific proteins from extracts of chicken, hamster, and field vole cells transformed by asv. two virus-specific proteins having molecular weights of 76,000 and 60,000 were found in all cell lines examined. the 76,000-molecular-weight protein, pr76, is the precursor to the internal core proteins of asv. the 60,000-molecular-weight (60k) transformation-specific antigen from each cell line wa ...1978209218
nature of rous sarcoma virus-specific rna in transformed and revertant field vole cells.cytoplasmic and polyribosomal rnas from rous sarcoma virus-transformed and phenotypically reverted field vole cells were fractionated by rate-zonal sedimentation and hybridized with a (3)h-labeled complementary dna viral probe to determine the size classes of virus-specific rna present in these cell types. in contrast to rous sarcoma virus-infected permissive avian cells, only two of three discrete species of virus-specific rna were detected in the cytoplasm of these vole cells. these included g ...1979219246
morphological revertants of an avian sarcoma virus-transformed mammalian cell line exhibit tumorigenicity and contain pp60src.the biological and biochemical properties of rous sarcoma virus-transformed and revertant field vole cells were investigated. revertant vole cells appear morphologically similar to normal, uninfected cells, yet, like transformed vole cells, they are fully capable of growing in agar suspension and producing tumors in athymic nude mice. these highly tumorigenic, yet morphologically normal appearing, vole cells express viral-specific antigens such as the gag gene product (pr76) but lack the env gen ...1979226988
alteration of free serum amino acids in voles infected with trypanosoma brucei serum amino acid pools of field voles, microtus montanus, were determined over a 24 hr period, and compared to values obtained from voles infected with trypanosoma brucei gambiense. the majority of amino acids in the control animals demonstrated a diurnal variation, peaking predominantly during the dark portion of the photoperiod. this trend was not evident in the infected animals. in addition, infected voles possessed an apparent state of hypoaminoacidemia, with levels of threonine, serine ...1977321737
the isolation and nature of campylobacters (microaerophilic vibrios) from laboratory and wild rodents.faeces voided by eight species of laboratory or feral rodents were cultured for campylobacters by means of selective methods. campylobacters were isolated from bank voles and from rats, but not from rabbits, laboratory mice, hamsters, guinea-pigs, field mice or field voles. in routine biochemical tests isolates from bank voles resembled a type of campylobacter fetus that causes infectious infertility in cattle; isolates from rats resembled campylobacter coli associated with swine dysentery. elec ...1977330861
genetically controlled differences in behaviour between cycling and non-cycling populations of field vole (microtus agrestis). 1977338556
free amino acids in brain, liver, and skeletal muscle tissue of voles infected with trypanosoma brucei gambiense.the concentrations of several acidic and neutral amino acids of brain, liver, and skeletal muscle were determined in field voles, microtus montanus, and compared to values obtained from voles harboring a chronic infection of trypanosoma brucei gambiense. all of the amino acids examined were found at comparable levels in brain tissue from both groups of animals with the exception of tyrosine, which was reduced by approximately 45% in the infected voles. similarly, the only difference noted in liv ...1977338875
enzymic markers of thyroid c cells in some rodents.the paper provides comparative data of the localization of histochemical reactions demonstrating the activities of alpha-glycerophosphate and succinate dehydrogenases, acid phosphatase, non-specific esterases and non-specific acetylcholinesterase in the c cells of thyroids of 26 animals belonging to 5 rodent species. the family muridae is represented by the wistar albino rat and albino mouse, the family microtidae by the bank vole clethrionomys glareolus (schreber 1780), the field vole microtus ...1977340363
[further studies on host range and life cycle of frenkelia microti from the field vole]. 1978358682
microtus agrestis and clethrionomys glareolus as experimental hosts of new world leishmania. 1979386966
babesia microti: morphology, distribution and host relationship in germany.a short review has been given about recent studies on babesia microti in natural animal host's near munich (southern germany). an infected area has been studied in order to elucidate the outdoor relationship between the local strains and their preferred hosts, the european field vole microtus agrestis. the seasonal variation of the parasites prevalence in voles shows a rise in the early summertime (71% of the catches infected). roundish forms predominate in the erythrocytes. multiplication never ...1979388948
anatomy of the cecum of the dwarf hamster (phodopus sungorus).the cecal anatomy of the dwarf hamster, phodopus sungorus, was investigated macroscopically and at the light, transmission electron microscopic and scanning electron microscopic levels. in addition, the arterial supply to the cecum and connecting mesenteries were elucidated. the cecum is divisible into an ampulla ceci and a corpus ceci. in the former, the differentiation of the ileocecal orifice and circumventing fold is described. scanning electron microscopically, the surface topography of the ...1979525821
effects of trypanosoma brucei gambiense infections in microtus montanus on susceptibility to ehrlich's tumors.trypanosoma brucei gambiense infections in the field vole microtus montanus increased susceptibility to ehrlich's tumor growth. whereas uninfected voles were totally resistant to intraperitoneal ehrlich's ascites tumor cell challenge, over 78% of the animals infected with the trypanosomes developed tumors after challenge. likewise, when ehrlich's ascites cells were injected subcutaneously to induce solid tumor formation, only 7% of uninfected controls developed tumors, whereas over 82% of trypan ...1976770326
plasma and pituitary levels of lh in field voles, microtus arvalis, reared under two different photoperiods.10 plasma lh levels were measured in voles reared under long (15 l: 9 d) or short (10 l: 14 d) light photoperiods by radioimmunoassay from a rat lh-anti ovine lh system. 20 the number of animals exhibiting a detectable lh level (i.e. greater than 1 ng per ml) and the mean plasma lh values were higher for long days than for short days in spite of a very large variability between animals. 30 the sensitivity of the hypophysis towards lh. rh increased after gonadectomy, but is not controlled by the ...1975772188
pregnancy blocking in the vole, microtus agrestis. i. effect of the social environment.pregnancy failed in a high proportion of newly mated microtus agrestis females when they were exposed to a strange male between 48 and 72 hr after mating with a stud male. this effect of the strange male was testosterone dependent. direct contact with the strange male was normally necessary, and even a single barrier of wire mesh between the female and strange male prevented the male from exerting his pregnancy-blocking effect. the results suggest that the stimuli mediating pregnancy blocking in ...1976775073
placental lactogen (chorionic mammotrophin) in the field vole, microtus agrestis, and the bank vole, clethrionomys glareolus.placental lactogen has been detected in the field vole, microtus agrestis, and the bank vole, clethrionomys glareolus, using a co-culture technique. in field voles this activity could be detected from about day 8 of pregnancy to shortly before term, and stimulated both mouse and vole mammary gland to secrete in vitro. partial immunological cross-reaction was detected in a radioimmunoassay system between rat prolactin and either extracts of vole pituitaries or media on which vole pituitaries had ...1976778321
immunodepression during trypanosoma brucei gambiense infections in the field vole, microtus montanus.the effects of trypanosoma brucei gambiense infections upon immune responses were examined in an outbred laboratory colony of field voles. microtus montanus. antibody levels to challenge with heterologous erythrocytes and bovine serum albumin were significantly depressed in infected animals. trypanosome infections impaired both primary and secondary humoral responses, although previously established specific antibody levels were not affected by infection. specific antibody-producing capabilities ...1976791544
[differentiation of nuclear structure in kidney tissue of the field-mouse, microtus agrestis, during perinatal development]. 1976799420
contribution to the understanding of the life-cycle of sarcocystis of the short-tailed vole microtus agrestis.attempts were made to transmit to clean voles a species of sarcocystis found in the muscle of the european short-tailed vole microtus agrestis, by inoculation of bradyzoites, feeding of infected muscle and feeding of faeces collected from voles and cats fed on infected vole flesh. transmission was not achieved. the results are discussed in the light of the positive findings reported in the older literature with other rodent sarcocystis spp. and the recent elucidation of the coccidian life-cycle ...1976827477
multiplication of mycobacterium tuberculosis and mycobacterium bovis in microtus agrestis (field vole).the multiplication of bacteria is examined by quantitative culture from the organs of two groups of field voles injected intraperitoneally with a large dose of m. tuberculosis or a small dose of m. bovis and killed at intervals during a period of up to 3 months after the injection. m. tuberculosis given in a dose of 7 x 10(5) viable units did not multiply, or multiplied only to a small extent. none of the animals in the group died from tuberculosis and the macroscopical lesions found at autopsy ...1976814785
isolation of a leptospira of the pomona serogroup from a field vole. 19751098271
anatomy of the cecum of the vole, microtus agrestis.the anatomy of the cecum of the vole, microtus agrestis, was investigated using macroscopic dissections, "wet" and dried total specimens, and correlated light, scanning and electron microscopy. the cecum of the vole reveals a series of structural differentiations including a mural lip in the ampulla ceci and a spiral fold in the corpus ceci. the mucosa covering the cecal wall possesses short, wide-opened crypts and differs from the classical descriptions of the large intestinal mucosa. fine stru ...1979391099
distribution of 18+28s ribosomal genes in mammalian situ hybridization with 3h 18s and 28s ribosomal rna from xenopus laevis has been used to study the distribution of dna sequences coding for these rnas (the nucleolus organizing regions) in the genomes of six mammals. several patterns of distribution have been found: 1) a single major site (rat kangaroo, seba's fruit bat), 2) two major sites (indian muntjac), 3) multiple sites in centromeric heterochromatin (field vole), 4) multiple sites in heterochromatic short arms (peromyscus eremicus), 5 ...19751104290
mating, ovulation and corpus luteum function in the vole, microtus agrestis.a study of the relationship between the mating vehaviour of the vole and the induction of ovulation and cl function is described. a single intromission or an injection of lh-rf constitute stimuli which induce ovulation, but normally give rise to cl that degenerate soon after formation. more prolonged mating, or mechanical stimulation of the vagina and cervix given after a separate ovulatory stimulus, result in the maintenance of the cl. mechanical genital stimulation is effective in inducing cl ...19751089153
[rodents trichomonads: morphology, life cycle and several ecologic features (polymastigina)].some morphological and cytochemical methods were applied to the study of the life cycle of trichomonas parasitizing the intestine of various rodents. the comparative study of trophonts morphology showed that the only species of the genus trichomonas--t. muris inhabits the intestine of mice, rats, field-voles, hamsters and susliks. the life cycle of t. muris includes three morphologically different stages: trophonts, pseudocysts and cysts. the pseudocysts and cysts are the infectious stages of t. ...1977865872
sex preference and species specificity of rodent (mus musculus and microtus arvalis pheromones.the behavioural response to the sex pheromones in the externally voided urine of field voles (microtus arvalis) and laboratory mice (cflp, cba strains) although specific for species showed no strain specificity. bladder urine (free of accessory sex-gland secretions) and the preputial glands of cflp and cba mice contain sex attractants. ether extracts made of blood of male cflp mice attracted cflp female mice.19751241218
host specificity of and cross-immunity between two strains of heligmosomoides polygyrus.the infectivity of wild and laboratory strains of heligmosomoides polygyrus (nematospiroides dubius) in laboratory mice and in three species of wild british rodent was compared. wild strains, of the subspecies h. p. polygyrus, were isolated from wild caught apodemus sylvaticus. only very low-level infections of the wild strains became established in laboratory mice. similar worm burdens of the laboratory strain became established in laboratory mice and a. sylvaticus, although infections in a. sy ...19911866189
the ultrastructure of the anterior pituitary gland of the vole, microtus agrestis, in normal and experimentally manipulated animals.the ultrastructural appearance of the various types of cell present in the anterior pituitary of the vole has been described. there was a great measure of similarity between the cytological picture in this species and in the rat. prolactotrophs contained the largest secretory granules, which were of variable shape; the granules of somatotrophs, whilst only slightly smaller than those of prolactotrophs, were invariably round, and of more uniform size; corticotrophs were represented by cells which ...19751102504
comparison of pancreatic digestive enzyme secretion induced by volatile fatty acids in mice, japanese field voles and goats.the secretory response of the exocrine pancreas to volatile fatty acids was investigated in the isolated preparations of mice, japanese field voles and goats. the amylase content in pancreases of the voles was half that of the mice, and the trypsin content was four times higher than that of the mice. amylase release to volatile fatty acids in the goats showed a dose-dependent relationship. the response in the voles was obtained by acetate and propionate, but preparations in the mice were ineffec ...19852411468
first evidence of the leptospirosis natural foci of the serotype saxkoebing in austria.the authors examined serologically and bacteriologically 129 wild living small mammals (9 species) in neumarkt, in styria. a total of 17 animals were positive (all microtus agrestis), of these 10 serologically (6 with l. saxkoebing, 3 with l. grippotyphosa and 1 with l. sorexjalna) and 7 bacteriologically. of the 7 isolated leptospirae-strains one did not survive up to typing, the 6 remaining were identified as l. saxkoebing. it is the first evidence of the existence of leptospirosis natural foc ...19892663645
use of recombinant vaccinia-rabies glycoprotein virus for oral vaccination of wildlife against rabies: innocuity to several non-target bait consuming species.the pathogenicity of a vaccinia recombinant virus expressing the rabies glycoprotein (vvtggrab) was tested in several wild animal species which could compete with the natural rabies host, the red fox (vulpes vulpes) in consuming vaccine baits in europe. the following species were included in this study: wild boar (sus scrofa), eurasian badger (meles meles), wood mouse (apodemus sylvaticus), yellow-necked mouse (apodemus flavicollis), bank vole (clethrionomys glareolus), common vole (microtus arv ...19892681844
linkage of the loci for glucose-6-phosphate dehydrogenase and for inosinic acid pyrophosphorylase to the x chromosome of the field-vole microtus has been proposed that there are strong selective pressures which have acted during the evolution of mammals to conserve the linkage of genes on the x chromosome. if so, loci that are known to be x-linked in one mammalian species should be x-linked in others. the loci for glucose-6-phosphate dehydrogenase (e.c. and for inosinic acid pyrophosphorylase (e.c. are known to be x-linked in a variety of mammals. the linkage of these loci to the x chromosome of the field-vole, micr ...19751167552
[blood parasites of small wild mammals in czechoslovakia].the author investigated 4,853 specimens of wild living small mammals (43 species: insectivora, chiroptera, lagomorpha, rodentia, carnivora) from czechoslovakia for blood parasites. trypanosomes were found in talpa europaea, sorex alpinus, crocidura suaveolens, myotis myotis, apodemus sylvaticus, apodemus flavicollis, mus musculus, rattus norvegicus, clethrionomys glareolus and microtus agrestis. babesia was detected in myotis emarginatus, myotis myotis, clethrionomys glareolus, microtus arvalis ...19751158292
highly specific antibody to rous sarcoma virus src gene product recognizes a novel population of pp60v-src and pp60c-src molecules.antiserum to the rous sarcoma virus (rsv)-transforming protein, pp60v-src, was produced in rabbits immunized with p60 expressed in escherichia coli. alpha p60 serum immunoprecipitated quantitatively more pp60v-src than did tumor-bearing rabbit (tbr) sera. when rsv-transformed cell lysates were preadsorbed with tbr serum, the remaining lysate contained additional pp60v-src, which was recognized only by reimmunoprecipitation with alpha p60 serum and not by tbr serum. in subcellular fractions of rs ...19852981886
role of the polar trophectoderm in determining the pattern of early post-implantation morphogenesis in mammals: evidence from development of the short-tailed field vole, microtus agrestis.the peri-implantational embryogenesis in the field vole, microtus agrestis, is described. implantation is interstitial, as it is in the mouse, but egg cylinder formation occurs by invagination of the blastocyst's embryonic pole and not (as in the mouse) by formation of a multilayered extra-embryonic ectoderm. this difference can be attributed to loss in the field vole of the central portion of the polar trophectoderm at the time of blastocyst attachment. in comparing early postimplantation devel ...19883070537
[incidence of coxiella burnetii and rickettsiae from the rmsf group in the sub-alpine region in austria].investigations were carried out on small mammals to determine further distribution of coxiella burnetii and rickettsia rmsf-group in the alpine and sub-alpine regions. in altitudes of 1,200-2,400 m antibodies against clethrionomys glareolus, apodemus flavicollis, sorex alpinus, sorex minutus and microtus agrestis were demonstrated. these findings emphasize that in the entire alpine and sub-alpine regions of austria the occurrence of c. burnetii and rmsf-group rickettsia is to be expected.19892744470
simple gatca repeats characterize the x chromosomal heterochromatin of microtus agrestis, european field vole (rodentia, cricetidae).the sex chromosomes of microtus agrestis are extremely large due to the accumulation of constitutive heterochromatin. we have identified two prominent satellite bands of 2.0 and 2.8 kb in length after haeiii and hinfi restriction enzyme digestion of genomic dna, respectively. these satellites are located on the heterochromatic long arm of the x chromosome as shown using microtus x mouse somatic cell hybrids. by in-gel hybridization with oligonucleotide probes, the organization of the two satelli ...19883282831
dnase i sensitivity of microtus agrestis active, inactive and reactivated x chromosomes in mouse-microtus cell hybrids.we isolated microtus agrestis-mouse somatic cell hybrid clones which had retained either the active or the inactive m. agrestis x chromosome. in both hybrid clones the x chromosomes retained their original chromatin conformation as studied by the in situ nick translation technique--the active x chromosome retained its high sensitivity to dnase i while the inactive one remained insensitive. a clone in which the hypoxanthine guanine phosphoribosyltransferase (hprt) gene had been spontaneously reac ...19883282832
evaluation of five cell types for the isolation of herpes simplex virus.five cells were evaluated in a comparative analysis for sensitivity, specificity, and rapidity in detecting the presence of herpes simplex virus hsv-1 and hsv-2. included in this study were human embryonic kidney (hek), rabbit kidney (rk), mrc-5, mink lung (ml), and microtus agrestis (umma). a total of 274 specimens from genital, throat, skin, or other sources that were submitted for hsv isolation were used in the study. the sensitivity of the different cells was assessed by the total number of ...19863013496
a golgi study of the sixth layer of the cerebral cortex. i. the lissencephalic brain of rodentia, lagomorpha, insectivora and chiroptera.a study of the morphological characteristics of the neurons in layer vi of the cerebral cortex was carried out using the rapid golgi method in several lissencephalic species including rodentia (rat, mouse, vole (microtus agrestis) and hamster), lagomorpha (rabbit), insectivora (hedgehog) and in the chiroptera the dwarf bat (pipistrellus pipistrellus). there was a basic uniformity in the structure of the sixth layer. main neuronal types in lamina via were large pyramidal neurons, triangular or at ...19863429306
immunohistochemical study of gastroenteropancreatic endocrine cells of the herbivorous japanese field vole, microtus montebelli.the gastroenteropancreatic (gep) endocrine cells of the japanese field vole were studied immunohistochemically. somatostatin-, 5-hydroxytryptamine-, glicentin-, glucagon-, bovine pancreatic polypeptide-, gastrin-, gastric inhibitory polypeptide-, cholecystokinin-, substance p-, secretin-, neurotensin- and insulin-immunoreactive cells were revealed. the characteristic findings of the regional distribution and relative frequency of these immunoreactive cells in the gep system of the vole were as f ...19863532246
[glycogen-containing cells in the maternal and embryonic portions of the placenta in the rat and the common vole microtus subarvalis].differentiation sequences and further transfiguration of glycogen-rich cells during placenta development were investigated for the rat and field vole microtus subarvalis (11-20 day gestation). the presence of glycogen is a characteristic feature of decidual cells located in the region of lateral sinusoids, as well as of metrial gland cells, secondary giant trophoblast cells and trophoblast cells in the connective zone of placenta. glycogen-containing metrial gland cells and trophoblast cells of ...19883072744
comparison of trypanosoma grayi-like isolates from west and east africa.three flagellates recently isolated from the hindguts of tsetse flies in west africa were compared with a previously described t. grayi-like trypanosome isolated in east africa. in media with microtus agrestis feeder layer cells, the flagellates developed into bloodstream-like trypomastigotes, which resembled the description of t. grayi from the blood of crocodiles. the results of isoenzyme electrophoresis suggest that the isolates are t. grayi-like trypanosomes, and that some variation exists b ...19911888219
dnase i sensitivity in facultative and constitutive situ nick translation allows the detection of dnase i sensitive and insensitive regions in fixed mammalian mitotic chromosomes. we have determined the difference in dnase i sensitivity between the active and inactive x chromosomes in microtus agrestis (rodent) cells, along both their euchromatic and constitutive heterochromatic regions. in addition, we analysed the dnase i sensitivity of the constitutive heterochromatic regions in mouse chromosomes. in microtus agrestis female cells the activ ...19853905296
in vitro cultivation of herpetosoma trypanosomes on embryonic fibroblasts and in semidefined cell-free medium.microtus agrestis embryo fibroblasts (maef) support the survival and multiplication at 37 c of the mammalian multiplicative forms of the herpetosoma trypanosomes trypanosoma microti, t. evotomys, t. musculi, and t. lewisi passaged from cultures on schneider's drosophila medium and of t. grosi from grace's medium. maef layers with parasites were maintained for a period of over 5 mo. a semidefined cell-free medium also supported the multiplication (at 37 c) of the mammalian forms of the same trypa ...19883288740
serological studies of british leptospiral isolates of the sejroe serogroup. iii. the distribution of leptospires of the sejroe serogroup in the british isles.some 94 strains of leptospires belonging to the sejroe serogroup isolated in the british isles were identified to the serovar level using specific factor sera. seventy strains were identified as leptospira interrogans serovar hardjo, 66 from cattle, 2 from pigs and 1 each from a sheep foetus and a human. twenty-four strains were identified as l. interrogans serovar saxkoebing, most strains were isolated from either wood mice, bank or field voles but strains were also isolated from badgers, a fox ...19873609169
basic carriers of trichophyton mentagrophytes var. gypseum in the sverdlovsk region.tests for myococarriers were made on 4.297 wild small mammals. clinical examinations only were carried out on 1,204 animals, both clinical and laboratory examinations on 3,093 animals. the suppurative form of trichophytosis was diagnosed in only one specimen of microtus oeconomus out of 1,204 animals examined. t. ectothrix microides was found in the hair taken from the focus. t. mentagrophytes var. gypseum was obtained by culture on nutrient media. no clinical signs of mycosis were found in 3,09 ...19873680938
seasonal dynamics of parasitocoenosis in field-vole (microtus arvalis, pallas, 1778) populations living in large-acreage lucerne fields. 19744454298
the concurrence of two species of nematodes of the genus heligmosomum in a field-vole (microtus arvalis) population in large-acreage lucerne fields. 19744454301
the induction of tumours with rous sarcoma virus in microtus agrestis: a preliminary note. 19704325786
louping-ill and semliki forest virus infections in the short-tailed vole microtus agrestis (l.). 19705530345
[the effect of methylnitronitrosoguanidine on dna synthesis in human and animal cells. inhibition of dna synthesis in asynchronous and synchronous cultured cells].methylnitronitrosoguanidine (mnng) is reported to inhibit dna synthesis in intact human cells, in the cells from patients with ataxia telangiectasia (at) or the cells from two rodent species. dna synthesis in different cell lines exhibits varying sensitivity to mnng inhibitory effect. 4-5-fold higher concentrations of mnng are required for 50% inhibition of dna synthesis in at cells or in field vole cells as compared with the concentration required for human cells or chinese hamster. the differe ...19853916233
the fine structure of babesia microti in the natural host, microtus agrestis. 19704992453
the repeated dna sequences of microtinae. i. microtus agrestis, microtus pennsylvanicus and ellobius lutescens. 19734128137
the fine structure of the sex vesicle and sex chromosome association in spermatocytes of mouse, golden hamster and field vole. 19665972764
[on the neurosecretory hypothalamo-hypophyseal system in rodents: the field mouse (microtus agrestis)]. 19655826828
trichophyton persicolor infection in the field vole and pipistrelle bat. 19666006211
hypothalamo-hypophysial control of ovulation in the vole (microtus agrestis). 19715558384
[studies of the ecology of some blood protozoa of wild small mammals in north germany (author's transl)].in 1977 and 1978, 696 small mammals of 9 different species were surveyed for protozoal blood parasites. the following parasites were found: hepatozoon sylvatici in apodemus flavicollis, h. erhardovae and trypanosoma evotomys in clethrionomys glareolus, t. microti and babesia microti in microtus agrestis and t. croicidurae in crocidura russula. h. erhardovae showed regularly high infection-rates (more than 70%) during all the years in 1977 and 1978. h. sylvatici was found only in 5.6% of the yell ...19806104423
[demonstration of the virus of hemorrhagic fever with renal syndrome in the organs of rodents from natural foci of the infection in the ussr and the serodiagnosis of the diseases].an indirect fluorescent antibody test (ifat) and an enzyme-immunological test (elisa) were used for the detection of hfrs virus in organs of rodents from hfrs foci in the ussr. the virus was found in 115 out of 1120 bank voles, 9 out of 92 redbacked voles, and 2 field voles examined. spontaneous infection-rate of bank voles in population varied from 1.3 to 100% correlating with the epidemiological situation in foci. ifat and elisa were successfully used for serodiagnosis of hfrs. examinations of ...19816121425
karyotype analysis and quantitation of viral transforming genes in rous sarcoma virus transformed, revertant, and retransformed field vole cells.comparative studies of the number of cellular chromosomes and viral genes, including the gene required for malignant transformation, were performed on several clones of rous sarcoma virus-transformed, revertant, and spontaneously retransformed field vole cells. the results of these studies indicate that no appreciable differences in either total viral gene equivalents or transforming gene sequences can be detected between transformed and revertant cell types, even though considerable differences ...19816262017
[transmission and course of parasitemia of babesia microti (hannover i strain) in the bank vole (clethrionomys glareolus) and field vole (microtus agrestis)].in germany only m. agrestis has been found to be naturally infected with b. microti while in other european countries several rodent species were found as mammalian hosts. in the laboratory c. glareolus got easily infected by german strains of b. microti, if parasitized blood was inoculated. but results of this method are not transferable to natural conditions. therefore this study was carried out. it was to prove whether c. glareolus is susceptible to b. microti transmitted by nymphs of i. rici ...19846150621
loss of tumorigenicity correlates with a reduction in pp60src kinase activity in a revertant subclone of avian sarcoma virus-infected field vole cells.we have recently isolated an interesting revertant subclone (revertant 866-4) of esv-infected field vole cells that is indistinguishable from uninfected vole cells with respect to its lack of transformed cell properties. these revertants are not only normal morphologically, but they do not grow in soft agar and are nontumorigenic in athymic nude mice. despite this lack of transformed cell properties, we have found that this cell line still contains pp60src at concentrations (0.30 microgram/mg ce ...19816261958
association of pp60src and src protein kinase activity with the plasma membrane of nonpermissive and permissive avian sarcoma virus-infected cells.the intracellular localization of pp60src and src protein kinase activity in avian sarcoma virus (asv)-infected chicken embryo fibroblasts and transformed and morphologically reverted field vole cells was examined by subcellular fractionation procedures. fractionation by differential centrifugation of dounce-homogenized cellular extracts prepared from vole cells showed that 83 to 91% of pp60src sedimented with particulate subcellular components from both transformed and revertant vole cells. a s ...19806257928
observations on uterine mast cells during early pregnancy in the vole, microtus agrestis.the vole, microtus agrestis, was chosen for this study of mast cells during early pregnancy because this species does not show spontaneous estrous cycles. mast cell numbers in the uterus are known to vary during the estrous cycle in some species (rat, cow, syrian hamster). mast cell changes during early pregnancy in the vole could not reflect hormonal changes which had occurred during a preceding estrous cycle. mast cells in the uterus (myometrium, endometrium, and mesometrial triangle) and ear ...19846375459
identification of a reservoir of leptospira interrogans serovar muenchen in voles (microtus agrestis and clethrionomys glareolus) in england.infection with leptospires of the australis serogroup is widespread in voles in southern england and the serovar identification of five randomly-selected isolates was determined by cross agglutination absorption. two isolates from short-tailed voles (microtus agrestis) and three isolates from bank voles (clethrionomys glareolus) were all identified as serovar muenchen. the only previous isolation of this serovar is that from a human in germany.19836372316
microtus species as new herbivorous laboratory animals: reproduction; bacterial flora and fermentation in the digestive tracts; and nutritional a study of the possible introduction of japanese field vole (microtus montebelli ) and hungarian voles (m. arvalis) as herbivorous experimental animals, the following biological characteristics were investigated: breeding and reproductive performance; bacterial flora and fermentation in the digestive tracts; and nutritional physiology. the animals are polyestrus , show postpartum estrus on the day of parturition, and there is little or no delay in implantation due to lactation, especially in ...19846377675
[the life-history of sarcocystis singaporensis zaman and colley, 1976 in the definitive and intermediate host (author's transl)].sporulated oocysts and sporocysts (9.3 x 7.3 micrometer) of sarcocystis singaporensis - isolated from freshly imported snakes (python reticulatus) - were fed to various animals to test their function as suitable intermediate hosts (nmri-mice, albino rats, meriones unguiculatus, golden hamsters, guinea pigs, mastomys natalensis, field-voles [microtus arvalis], pigeons, and chickens). only in rats were muscle-cysts formed. the optimal dose was 150 sporocysts per rat. two generations of merogony co ...19806771934
increase in the phosphotransferase specific activity of purified rous sarcoma virus pp60v-src protein after incubation with atp plus mg2+.pp60v-src, the product of the rous sarcoma virus src gene, was partially purified by immunoaffinity chromatography from extracts of rous sarcoma virus-transformed field vole cells. incubation of this preparation with atp plus mg2+ and subsequent repurification by chromatography on hexylamine-agarose resulted in a net increase in the specific activity of the src protein kinase. this increase in phosphotransferase activity was detected by using a variety of substrates including casein, tubulin, an ...19836314123
prevalence of pneumocystis carinii delanoë & delanoë, 1912 in rodents in denmark.pneumocystis carinii has been found in 17 of 90 (19%) wild living trapped brown rats (rattus norvegicus). the positive brown rats originated from about 25% of the trapping localities examined. among brown rats of two breeding stocks, h and s, pneumocysts were found in 43.5% of the h rats, but in none of the s rats. by keeping brown h rats on a low protein diet for 8 weeks, it was possible to augment the prevalence to 53.8%. pneumocystis carinii has furthermore been found in one of eight (12.5%) ...19806965782
allozyme variation in natural populations of field vole (microtus agrestis l.). i. survey of the 'semi-stable' population in southern sweden. 19806991456
a note on the ploidy of purkinje cells; sex chromatin in field vole (microtus agrestis) and man.examination of sex chromatin bodies in purkinje cell nuclei suggests only a diploid chromosomal content: there is no increase in number or size above that seen in diploid cells.19806990292
allozyme variation in natural populations of field vole (microtus agrestis l.). iii. survey of a cyclically density-varying population. 19807002878
allozyme variation in natural populations of field vole (microtus agrestis l.). ii. survey of an isolated island population. 19807002877
cerebrospinal fluid-contacting area in the pineal recess of the vole (microtus agrestis), guinea pig (cavia cobaya), and rhesus monkey (macaca mulatta).the ventricular lining in the pineal recess of the vole (microtus agrestis), guinea pig (cavia cobaya) and rhesus monkey (macaca mulatta) was investigated light and electron microscopically. deep in the pineal recess of all three species the ependymal lining exhibits interruptions. a varying proportion of pinealocytes penetrates through this ependymal area, so that the surface of the protruding cells is directly exposed to the cerebrospinal fluid (csf). at their base, these cells are anchored in ...19806996824
[organization of mammalian genome replication: data on the high rate of dna replication in replicons of structural heterochromatin].by the autoradiography, the increase of the mean rate of replication fork movement in dna was shown for human skin fibroblasts during s-period induced by reseeding the stationary culture; the portion of high-rate replicons growing parallel to the increase in numbers of cells, in which the constitutive heterochromatin was replicating. furthermore, the heterogeneity of replicons was investigated in regard to the rate of dna chain growth in asynchronous cell populations of human and three rodent sp ...19806998067
the development of field vole (microtus agrestis) and mouse blastocysts in vitro: a study of trophoblast cell migration.outgrowth of field vole blastocysts and their constituent tissues in vitro results in the appearance of trophoblast giant cell monolayers plus migratory cells of two types. the large migratory cells, which are never seen in mouse outgrowths, resemble trophoblast giant cells in morphology and dna content. they probably correspond to giant cells which have been observed to migrate throughout the endometrium of vole implantation sites in vivo. it is suggested that their appearance in vitro may depe ...19807003579
corynebacterium kutscheri infection in wild voles (microtus agrestis). 19817013898
comparative effect of the three rodenticides warfarin, difenacoum and brodifacoum on eight rodent species in short feeding periods.short laboratory feeding tests were carried out with the anticoagulants warfarin, difenacoum, and brodifacoum on a number of european rodent species: clethrionomys glareolus, microtus agrestis, m. arvalis, apodemus flavicollis, a. sylvaticus, mus musculus, rattus rattus and r. norvegicus. it was found that the toxicity to all species was highest with brodifacoum and lowest with warfarin, and that only 0.005% brodifacoum would give a complete mortality in most species after one day's feeding. the ...19817019316
[epidemiological survey in a natural focus of rickettsia transmitted by ixodes ricinus in switzerland (author's transl)].a spotted fever group rickettsia referred to as "swiss agent" was detected in 8.5% of ixodes ricinus collected in a marshy forest of the swiss plateau. this organism, in its tick vector, was found to be transmitted stadially as well as ovarially; of 433 larval, nymphal or adult ticks from 17 parental tick females, all were infected. a total of 3,612 larval i. ricinus were collected from 168 small mammals including 127 clethrionomys glareolus, 29 apodemus sylvaticus and flavicolis, 7 microtus agr ...19817020557
infections with blood parasites in the small british rodents apodemus sylvaticus, clethrionomys glareolus and microtus agrestis.three populations of small wild british rodents were studied by capture-recapture methods over a period of 3 years, a fourth group was studied for 1 year and a fifth was sampled annually for 4 years. blood smears were taken from 3 species of rodents: the woodmouse apodemus sylvaticus, the bank vole clethrionomys glareolus (and an island sub-species c.g. skomerensis) and the short-tailed vole microtus agrestis. the smears were examined microscopically. four genera of haemoparasites babesia, hepat ...19817022328
hepatic microsomal alterations during chronic trypanosomiasis in the field vole, microtus montanus.the field vole, microtus montanus, was used as a model system to evaluate the chronic effects of infection by trypanosoma brucei gambiense on hepatic mixed-function oxidase activity. at day 28 post inoculation there was a 97% increase in liver wet weight per g body weight. a portion of the increase (21%) was accounted for by tissue edema which occurred after day 14 of infection. total hepatic cytochrome p-450 content and related total tissue mixed-function oxidase activities were decreased to ab ...19827050701
[contribution to the knowledge of taenia crassiceps (zeder, 1800) rudolphi, 1810 (cestoda, taeniidae) (author's transl)].the occurrence of taenia crassiceps in naturally infected dogs is mentioned, and a brief description of the gravid proglottids is given. oral infection of several rodent species with eggs showed that the field vole (microtus arvalis) is the most susceptible intermediate host for this tapeworm. the cysticercus often develops in the brain of the rodent and causes disturbances of coordinated movements. two foxes (vulpes vulpes) were infected with cysticerci of the strains cola and giks. these strai ...19817314812
serological evidence for the reservoir hosts of cowpox virus in british wildlife.the reservoir host of cowpox virus in western europe is not known, but epidemiological evidence from human and feline infections indicates that the virus is probably endemic in small wild rodents. therefore, serum and tissue samples were collected from a variety of wild british mammals and some birds, and tested for evidence of orthopoxvirus infection. antibody reacting with cowpox virus was detected in 9/44 (20%) bank voles (clethrionomys glareolus), 8/24 (33%) field voles (microtus agrestis), ...19957641833
a serological proof of adiaspiromycosis in wild rodents.using serological and histological methods, we examined 92 specimens of small, wild rodents (apodemus sylvaticus, clethrionomys glareolus, micromys minutus, microtus agrestis, m. arvalis, mus musculus and pitymys subterraneus) for the incidence of adiaspiromycosis. in addition, we examined 16 white, laboratory mice after their exposure to a natural biotope in which the incidence of adiaspiromycosis had been confirmed earlier. the results of serological tests were in correlation with those obtain ...19807450616
prevalence of antibodies to borrelia burgdorferi in danish rodents.six rodent species from six danish localities were examined for antibodies to borrelia burgdorferi by immunofluorescence assay (ifa). a total of 1097 specimens were tested using a serum titre of 1:8. the wild mice apodemus sylvaticus and a. flavicollis had high prevalences of b. burgdorferi antibodies of 42.1% and 27.9% respectively, but the vole microtus agrestis also showed an exceptionally high prevalence of 32.7%. clethrionomys glareolus had a low average prevalence of 17.4%. the lowest and ...19957612254
temporal dynamics of puumala virus antibody prevalence in voles and of nephropathia epidemica incidence in humans.puumala (puu) virus is the etiologic agent of nephropathia epidemica (ne) in humans. this disease is highly endemic in vasterbotten county, sweden, with an annual incidence of 19.2 (range 3.7-37.4) per 100,000 inhabitants. voles are considered to be both the main reservoir and the vector of puu virus. a total of 3,591 rodents (mainly clethrionomys glareolus, c. rufocanus, and microtus agrestis) trapped in vasterbotten between 1979 and 1987 were tested for the presence of puu virus antibodies by ...19957677213
in vitro fertilization and embryo development of japanese field voles (microtus montebelli).optimal conditions for in vitro fertilization of japanese field voles (microtus montebelli) were analysed. the medium used was a modified krebs-ringer bicarbonate devised for in vitro fertilization in rats. ovulated eggs and epididymal spermatozoa were co-incubated in vitro at 37 degrees c under 5% co2 in air for 6 h, and the eggs were fixed with 2.5% (w/v) glutaraldehyde, stained with 0.25% (v/v) acetolacmoid and examined for evidence of fertilization at the pronuclear stage. although the ferti ...19957636806
grahamella in small woodland mammals in the u.k.: isolation, prevalence and host specificity.bacteria isolated from the blood of small woodland mammals were identified as members of the genus grahamella. the prevalence of grahamella infection among the 37 small mammals examined, detected by cultivation of blood samples, was 62%. this figure is somewhat higher than previous reports. further characterization of the isolates, based on restriction enzyme analysis of the 16s rrna gene, serological reactivity and dna hybridization studies, revealed three distinct grahamella species. one of th ...19947944677
mortality factors in a cyclic vole population.the causes of cyclic fluctuations in microtine rodent populations are still a bone of contention. in particular, the actual causes of mortality in the different phases of the 3-4-year vole are an enigma. we present results from studies of radio-collared voles (microtus agrestis, m. rossiaemeridionalis and clethrionomys glareolus), which show that small mustelid predation was the major mortality factor of voles in the decline phase, but had less importance in the increase phase of the 3-year popu ...19957644548
development of early-stage embryos of the japanese field vole, microtus montebelli, in vivo and in vitro.although ovulation could be easily induced in the japanese field vole by administering pregnant mares' serum gonadotrophin and hcg, the number of embryos obtained varied from 1 to 47 (mean, 9.6). one-cell embryos were small (57.8-63.3 microns in diameter; mean, 61.0 microns) compared with those in other mammals. development of the preimplantation vole embryos in vivo was similar to that of mouse embryos. the first cleavage occurred between 24 and 26 h after mating. the second cleavage was betwee ...19947966023
colonization history of north european field voles (microtus agrestis) revealed by mitochondrial dna.the genetic structure of field vole (microtus agrestis) populations from northern europe was examined by restriction fragment length polymorphisms of mitochondrial dna (mtdna) in 150 individuals from 67 localities. a total of 83 haplotypes was observed, most of which were rare and highly localized geographically. overall nucleotide diversity was high (1.34%), but showed a tendency to decrease with higher latitude. two major mtdna lineages differing by 2% in nucleotide sequence were identified. a ...19957663749
prevalence of cryptosporidium sp. in peak density microtus agrestis, microtus oeconomus and clethrionomys glareolus populations.the prevalence of cryptosporidia was determined in high density populations of microtus agrestis, microtus oeconomus, and clethrionomys glareolus in finland. one of 131 m. agrestis and one of 41 c. glareolus each were found to be infected with cryptosporidium sp.; none were found in 43 microtus oeconomus. these apparently healthy voles had neither signs of clinical disease nor histopathological changes in intestines.19948151812
[adrenocortical zonation in the laboratory japanese field vole (microtus montebelli)].adrenocortical zonation in the laboratory japanese field vole (microtus montebelli) was investigated histologically with special attention to the sex and species specific differences. the whole cortex and zona reticularis showed clear sex differences. they were thicker in females than in males. in this respect the vole was similar to the mouse and rat, but different from the syrian hamster and mastomys. male cortices were characterized by thick z. fasciculata and meagre z. reticularis, while bot ...19938519305
production of prostaglandins d2 and e2 by mouse fibroblasts and astrocytes in culture caused by trypanosoma brucei brucei products and endotoxin.a study was made to characterize the effects of living trypanosoma brucei brucei and its products on prostaglandin d2 (pgd2) and pge2 production by fibroblasts and astrocytes. cultured fibroblasts were prepared from microtus agrestis embryos and astrocyte cultures were prepared from neonatal rats. the cultures were maintained in low-endotoxin or defined media (i.e. endotoxin-free). the pg production was compared with and studied in combination with a defined lipopolysaccharide (lps) from escheri ...19948036236
transmission of borrelia burgdorferi s.l. from mammal reservoirs to the primary vector of lyme borreliosis, ixodes ricinus (acari: ixodidae), in sweden.factors regulating prevalence of borrelia burgdorferi s.l. johnson, schmid, hyde, steigerwalt & brenner in ixodes ricinus (l.) were examined during 1991-1992 at bogesund, near stockholm in south-central sweden. nine species of small and medium-sized mammals (sorex araneus l., s. minutus l., neomys fodiens pennant, clethrionomys glareolus [schreber], microtus agrestis [l.], apodemus sylvaticus [l.], a. flavicollis [melchior], lepus europaeus pallas, l. timidus l.) were found to infect feeding tic ...19947815401
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