| possible toxic effects of the marine blue-green alga, spirulina subsalsa, on the blue shrimp, penaeus stylirostris. | | 1978 | 103972 |
| toxicity of the organophosphorous insecticide metamidophos (o,s-dimethyl phosphoramidothioate) to larvae of the freshwater prawn macrobrachium rosenbergii (de man) and the blue shrimp penaeus stylirostris stimpson. | | 1989 | 2775897 |
| transformation of primary cultures of shrimp (penaeus stylirostris) lymphoid (oka) organ with simian virus-40 (t) antigen. | primary cultures of lymphoid (oka) organ from penaeus stylirostris were transformed with naked or lipofectin-mediated psv-3 neo, a shuttle vector containing the tumor (t) antigen gene from simian virus-40. the transformed cells, oktr-1 and oktr-23, exhibited the following characteristics: rounded morphology forming grapelike aggregates, loosely adhesive, increased growth rate in medium-199, resistance to g-418 (a neomycin analog marker in the shuttle vector), cloning efficiencies of 68.7% and 36 ... | 1995 | 7724618 |
| epizootiology, distribution and the impact on international trade of two penaeid shrimp viruses in the americas. | marine penaeid shrimp are effected by approximately twenty viruses, the majority of which were discovered as a result of their negative effects on aquaculture. in the americas, infectious hypodermal and haematopoietic necrosis (ihhn) virus and taura syndrome (ts) virus have had a significant negative impact on aquaculture industries and, in one instance, on a commercial fishery. both viruses have become widely distributed as a consequence of the movement of host stocks for aquaculture. ihhn viru ... | 1996 | 8890382 |
| toxic factors of vibrio strains pathogenic to shrimp. | vibriosis is a major disease problem in shrimp aquaculture. 'syndrome 93' is a seasonal juvenile vibriosis caused by vibrio penaeicida which affects litopenaeus stylirostris in grow-out ponds in new caledonia. this study assessed the toxic activities of extracellular products (ecps) from v. penaeicida, v. alginolyticus and v. nigripulchritudo using in vivo injections in healthy juvenile l. stylirostris (= penaeus stylirostris) and in vitro assays on shrimp primary cell cultures and the fish cell ... | 2000 | 10782343 |
| rapid and sensitive pcr detection of vibrio penaeicida, the putative etiological agent of syndrome 93 in new caledonia. | experimental infections of penaeus (litopenaeus) stylirostris were performed with a vibrio penaeicida strain (am101) isolated in new caledonia from syndrome 93 diseased shrimp. cumulative mortalities resulting from intramuscular injection or immersion of shrimp in bacterial suspensions demonstrated high virulence for this bacterial strain and suggested that v. penaeicida could be the etiological agent of syndrome 93. the median lethal dose (ld50) for am101 was 1.3 x 10(4) cfu (colony forming uni ... | 2000 | 10782344 |
| infectious hypodermal and hematopoietic necrosis virus of shrimp is related to mosquito brevidensoviruses. | we purified and sequenced infectious hypodermal and hematopoietic necrosis virus (ihhnv), a small dna virus of shrimp, from wild penaeus stylirostris. the virion has a buoyant density of 1.45 as determined by cesium chloride gradient. analysis of 3873 nucleotides of the viral genome revealed three large open reading frames (orfs) and parts of the noncoding termini of the viral genome. the left, mid, and right orfs on the complementary (plus) strand have potential coding capacities of 666 amino a ... | 2000 | 11062047 |
| detection and quantification of infectious hypodermal and hematopoietic necrosis virus in penaeid shrimp by real-time pcr. | a real-time pcr method using a fluorogenic 5' nuclease assay and a pe applied biosystems geneamp 5700 sequence detector was developed to detect infectious hypodermal and hematopoietic necrosis virus (ihhnv) in penaeid shrimp. a pair of pcr primers to amplify an 81 bp dna fragment and a fluorogenic probe (taqman probe) were selected from orf1 (open reading frame 1) of the ihhnv genome. the primers and taqman probe used in this assay were shown to be specific for ihhnv and did not react with eithe ... | 2001 | 11324819 |
| hemolymph analysis and evaluation of newly formulated media for culture of shrimp cells (penaeus stylirostris). | creation of a shrimp cell line has been an elusive goal. this failure may be due to the composition of the cell culture medium, which may be inadequate to support primary cultured cells. shrimp hemolymph should contain the nutritional components needed to support cell growth and division. we report here the comprehensive biochemical analysis of hemolymph from the blue shrimp, penaeus stylirostris (litopenaeus stylirostris) (see holthuis, l. b. shrimps and prawns of the world, in: fao species cat ... | 2001 | 11515962 |
| rapd markers as predictors of infectious hypodermal and hematopoietic necrosis virus (ihhnv) resistance in shrimp (litopenaeus stylirostris). | random amplified polymorphic dna (rapd) fingerprints of two shrimp populations (litopenaeus stylirostris) were compared to find genetic marker(s) that may be associated with infectious hypodermal and hematopoietic necrosis virus (ihhnv) resistance or susceptibility. of the 100 10-mer random primers and 100 intersimple-sequence repeat (issr) primers screened, five provided markers specific to the super shrimp population and three provided markers specific to the wild caught population. the two po ... | 2002 | 11908650 |
| the lipopolysaccharide and beta-1,3-glucan binding protein gene is upregulated in white spot virus-infected shrimp (penaeus stylirostris). | pattern recognition proteins such as lipopolysaccharide and beta-1,3-glucan binding protein (lgbp) play an important role in the innate immune response of crustaceans and insects. random sequencing of cdna clones from a hepatopancreas cdna library of white spot virus (wsv)-infected shrimp provided a partial cdna (psest-289) that showed similarity to the lgbp gene of crayfish and insects. subsequently full-length cdna was cloned by the 5'-race (rapid amplification of cdna ends) technique and sequ ... | 2002 | 12072514 |
| isolation of differentially expressed genes from white spot virus (wsv) infected pacific blue shrimp (penaeus stylirostris). | to isolate novel cellular factors that are activated or repressed upon wsv infection, the rna fingerprints of healthy and wsv infected blue shrimp ( penaeus stylirostris) were compared using the mrna differential display technique. thirty-two unique differentially expressed, and one constitutively expressed, cdna sequences were retrieved. six of 32 cdnas showed similarities with the database entries: cdna 10g32-142 to a shrimp arginine kinase, 22c48-201 to shrimp mitochondrial atpase gene; 22c47 ... | 2002 | 12209318 |
| genetic variation and immunohistochemical differences among geographic isolates of taura syndrome virus of penaeid shrimp. | taura syndrome virus (tsv) is an important virus infecting penaeid shrimp in the western hemisphere. genetic variation and immunohistochemical differences of 20 tsv isolates collected from the usa, taiwan, mexico and nicaragua were compared. capsid protein genes cp1 (546 bp) and cp2 (584 bp) were amplified by rt-pcr and the cdnas were sequenced. pairwise comparison of nucleotide sequences showed a 0-2.4% difference in cp1 and a 0-3.5% difference in cp2. phylogenetic analyses clustered the tsv is ... | 2002 | 12466489 |
| detection of taura syndrome virus (tsv) strain differences using selected diagnostic methods: diagnostic implications in penaeid shrimp. | anecdotal industry reports of taura syndrome (ts) epizootics in a taura syndrome virus (tsv) tolerant strain of penaeus stylirostris and collected evidence of field ts epizootics in p. stylirostris suggested that a distinct new tsv strain might have emerged since 1994. the ecuadorian 1992 tsv genome published in genbank is virtually identical to the hawaiian 1994 tsv isolate (hi94tsv) used as reference throughout this investigation. three other geographic and year isolates of tsv from naturally ... | 2002 | 12517000 |
| identification of differentially expressed genes in shrimp (penaeus stylirostris) infected with white spot syndrome virus by cdna microarrays. | white spot syndrome virus (wssv) is currently the most important viral pathogen infecting penaeid shrimp worldwide. although considerable progress has been made in characterizing the wssv genome and developing detection methods, information pertaining to host genes involved in wssv pathogenesis is limited. we examined the potential of cdna microarray analysis to study gene expression in wssv-infected shrimp. shrimp cdnas were printed as low-density arrays on glass slides and were hybridized with ... | 2003 | 14648293 |
| genetic markers applied in regression tree prediction models. | classification and regression tree (cart) modelling was used to determine infectious hypodermal and haematopoietic necrosis virus (ihhnv) resistance and susceptibility in penaeus stylirostris. in a previous study, eight random amplified polymorphic dna (rapd) markers and viral load values using real-time quantitative pcr were obtained and used as the training data set in order to create numerous regression tree models. specifically, the genetic markers were used as categorical predictor variable ... | 2004 | 14731230 |
| involvement of penaeidins in defense reactions of the shrimp litopenaeus stylirostris to a pathogenic vibrio. | the present study reports for the first time the involvement of an antimicrobial peptide in the defense reactions of a shrimp infected by a pathogenic vibrio, vibrio penaeicida. new members of the penaeidin family were characterized in the shrimp litopenaeus stylirostris by rt-pcr and race-pcr from hemocyte total rnas, and by mass spectrometry detection and immunolocalization of mature peptides in shrimp hemocytes. in infected shrimps, bacteria and penaeidin distribution colocalized in the gills ... | 2004 | 15095016 |
| molecular cloning and characterisation of a pattern recognition molecule, lipopolysaccharide- and beta-1,3-glucan binding protein (lgbp) from the white shrimp litopenaeus vannamei. | a lipopolysaccharide- and beta-1,3-glucan binding protein (lgbp) cdna was cloned from the haemocyte and hepatopancreas of white shrimp litopenaeus vannamei using oligonucleotide primers and rt-pcr. both 3'- and 5'-regions were isolated by rapid amplification of cdna end race method. analysis of nucleotide sequence revealed that the cdna clone has an open reading frame of 1101 bp encoding a protein of 367 amino acids including a 17 amino acid signal peptide. the calculated molecular mass of the m ... | 2005 | 15561560 |
| identification of genes that are differentially expressed in hemocytes of the pacific blue shrimp (litopenaeus stylirostris) surviving an infection with vibrio penaeicida. | considerable progress has been made in the field of invertebrate immunity through the characterization of genes involved in the response to infection and/or stress. however, the mechanisms by which commercially important marine invertebrates can successfully survive an infection remain largely unknown. for the first time in an invertebrate model, we have searched to discover genes involved in the survival capacity of shrimp using the highly pathogenic bacteria, vibrio penaeicida. in the present ... | 2005 | 15728333 |
| molecular epidemiology of vibrio nigripulchritudo, a pathogen of cultured penaeid shrimp (litopenaeus stylirostris) in new caledonia. | a collection of 57 isolates of vibrio nigripulchritudo from either diseased or healthy shrimp and from shrimp farms environment was studied in order to gain a better understanding of the epidemiology of this pathogen, notably isolated from two distinct shrimp disease complexes. molecular typing using two different techniques, arbitrarily primed pcr (ap-pcr) and multi-locus sequence typing (mlst), studied together with experimental pathology data allowed a relevant epidemiological insight into th ... | 2006 | 16413158 |
| quantification of vibrio penaeicida, the etiological agent of syndrome 93 in new caledonian shrimp, by real-time pcr using sybr green i chemistry. | shrimp farming is a small but growing industry in new caledonia. since 1993, "syndrome 93" has been affecting new caledonian shrimp farming industry every cold season, causing severe epizootic mortalities in grow-out ponds and significant losses. highly pathogenic strains of vibrio penaeicida are considered the etiological agent of the disease in litopenaeus stylirostris. on one hand, studies demonstrated that healthy shrimp may carry v. penaeicida for weeks with a high overall prevalence, regar ... | 2006 | 16616385 |
| viral interference between infectious hypodermal and hematopoietic necrosis virus and white spot syndrome virus in litopenaeus vannamei. | white spot syndrome virus (wssv) is highly virulent and has caused significant production losses to the shrimp culture industry over the last decade. infectious hypodermal and hematopoietic necrosis virus (ihhnv) also infects penaeid shrimp and, while being less important than wssv, remains a major cause of significant production losses in litopenaeus vannamei (also called penaeus vannamei) and l. stylirostris (also called penaeus stylirostris). these 2 viruses and their interactions were previo ... | 2006 | 17140141 |
| metal content of the gulf of california blue shrimp litopenaeus stylirostris (stimpson). | the blue shrimp litopenaeus stylirostris is the main target species of the gulf of california shrimping fleet, and its heavy metal content might be of concern for human health because of the increasing contamination of the gulf. the cd content of shrimp caught by commercial trawlers ranged from 0.38 to 1.05 microg/g and the mean value was significantly lower in the northern fishing grounds. pb ranged from 3.19 to 9.59 microg/g and was significantly higher in the northern area. there were no sign ... | 2007 | 17639322 |
| molecular cloning and characterisation of a pattern recognition protein, lipopolysaccharide and beta-1,3-glucan binding protein (lgbp) from chinese shrimp fenneropenaeus chinensis. | a pattern recognition protein (prp), lipopolysaccharide and beta-1,3-glucan binding protein (lgbp) cdna was cloned from the haemocyte of chinese shrimp fenneropenaeus chinensis by the techniques of homology cloning and race. analysis of nucleotide sequence revealed that the full-length cdna of 1,275 bp has an open reading frame of 1,098 bp encoding a protein of 366 amino acids including a 17 amino acid signal peptide. sequence comparison of the deduced amino acid sequence of f. chinensis lgbp sh ... | 2009 | 18163220 |
| correlation between detection of a plasmid and high-level virulence of vibrio nigripulchritudo, a pathogen of the shrimp litopenaeus stylirostris. | vibrio nigripulchritudo, the etiological agent of litopenaeus stylirostris summer syndrome, is responsible for mass mortalities of shrimp in new caledonia. epidemiological studies led to the suggestion that this disease is caused by an emergent group of pathogenic strains. genomic subtractive hybridization was carried out between two isolates exhibiting low and high virulence. our subtraction library was constituted of 521 specific fragments; 55 of these were detected in all virulent isolates fr ... | 2008 | 18359828 |
| a relationship between antimicrobial peptide gene expression and capacity of a selected shrimp line to survive a vibrio infection. | understanding of antimicrobial defence mechanisms of penaeid shrimp should help in the design of efficient strategies for the management and disease control in aquaculture. in this study, we have specifically analysed the expression in circulating hemocytes of antimicrobial peptides (amps) encoding genes, such as pen2 and pen3, alf, crustin, lysozyme and a putative cysteine-rich peptide. we evidenced a relationship between the level of expression of some amps and the successful response of the s ... | 2008 | 18486974 |
| evidence of multiple retrotransposons in two litopenaeid species. | retrotransposons encompass a specific class of mobile genetic elements that are widespread across eukaryotic genomes. the impact of the varied types of retrotransposons on these genomes is just beginning to be deciphered. in a step towards understanding their role in litopenaeid shrimp, we have herein identified nine non-ltr retrotransposons, among which several appear to exist outside the standard defined clades. two litopenaeus stylirostris elements were discovered through degenerate pcr ampli ... | 2008 | 18557973 |
| detection of the yellow-head virus (yhv) in wild blue shrimp, penaeus stylirostris, from the gulf of california and its experimental transmission to the pacific white shrimp, penaeus vannamei. | | 2008 | 19017072 |
| validation of reference genes for quantitative measurement of immune gene expression in shrimp. | to accurately measure the relative expression of a target gene, mrna expression data is routinely normalized with reference to an internal control gene. we examined the transcriptional stability of four internal control genes, beta-actin, glyceraldehyde-3 phosphate dehydrogenase (gapdh), elongation factor1-alpha (ef1-alpha), and 18s ribosomal rna (18s rrna) while measuring the mrna expression of a gene encoding a pattern recognition protein, lipopolysaccharide and glucan binding protein (lgbp) g ... | 2009 | 19297025 |
| simple immunoblot and immunohistochemical detection of penaeus stylirostris densovirus using monoclonal antibodies to viral capsid protein expressed heterologously. | penaeus stylirostris densovirus (pstdnv), called formerly infectious hypodermal and hematopoietic necrosis virus (ihhnv), is an important shrimp pathogen which can cause mortality in the blue shrimp penaeus (litopenaeus) stylirostris and stunting in the whiteleg shrimp penaeus (litopenaeus) vannamei. five monoclonal antibodies (mabs) were produced against the 37kda capsid protein 3 (cp3) of pstdnv expressed heterologously in the form of a fusion protein with glutathione-s-transferase called gst- ... | 2009 | 19654023 |
| rapid and sensitive detection of penaeus monodon nucleopolyhedrovirus by loop-mediated isothermal amplification. | loop-mediated isothermal amplification (lamp) is a novel, sensitive and rapid method for amplification of nucleic acids under isothermal conditions. in this report, a lamp method was developed for detection of penaeus monodon nucleopolyhedrovirus (pemonpv), known previously as monodon baculovirus (mbv), using a set of six primers designed to specifically recognize the pemonpv polyhedrin gene. the optimized time and temperature conditions for the lamp assay were 60 min at 63 degrees c. the sensit ... | 2009 | 19703492 |
| rapid and sensitive detection of penaeus monodon nucleopolyhedrovirus (pemonpv) by loop-mediated isothermal amplification combined with a lateral-flow dipstick. | several methods such as traditional pcr or nested-pcr, immuno assay and histopathology have been developed for detection of penaeus monodon nucleopolyhedrovirus (pemonpv) formerly called monodon baculovirus (mbv). however, these methods have various disadvantages including low sensitivity, long assay time, use of toxic substances or unsuitability for field diagnosis. loop-mediated isothermal amplification of target nucleotide sequences under isothermal conditions, combined with amplicon detectio ... | 2010 | 19818396 |
| effect of probiotic pediococcus acidilactici on antioxidant defences and oxidative stress of litopenaeus stylirostris under vibrio nigripulchritudo challenge. | antioxidant defences and induced oxidative stress tissue damage of the blue shrimp litopenaeus stylirostris, under challenge with vibrio nigripulchritudo, were investigated for a 72-h period. for this purpose, l. stylirostris were first infected by immersion with pathogenic v. nigripulchritudo strain sfn1 and then antioxidant defences: superoxide dismutase (sod), catalase (cat), glutathione peroxidase (gpx), total antioxidant status (tas), glutathiones and induced tissue damage (mda and carbonyl ... | 2010 | 20045735 |
| stylicins, a new family of antimicrobial peptides from the pacific blue shrimp litopenaeus stylirostris. | the present study reports the characterization of ls-stylicin1, a novel antimicrobial peptide from the penaeid shrimp, litopenaeus stylirostris. the predicted mature peptide of 82 residues is negatively charged (theoretical pi=5.0) and characterized by a proline-rich n-terminal region and a c-terminal region containing 13 cysteine residues. the recombinant ls-stylicin1 has been isolated in both monomeric and dimeric forms. both display strong antifungal activity against fusarium oxysporum (1.25 ... | 2010 | 20061030 |
| protection of blue shrimp (litopenaeus stylirostris) against the white spot syndrome virus (wssv) when injected with shrimp lysozyme. | in this study we found that a blue shrimp (litopenaeus stylirostris) lysozyme gene (lslzm) was up-regulated in wssv-infected shrimp, suggesting that lysozyme is involved in the innate response of shrimp to this virus. shrimp were intramuscularly injected with lslzm protein to identify how this recombinant protein protects l. stylirostris from wssv infection and to determine how this protein influences nonspecific cellular and humoral defense mechanisms. higher survival rates and a lower viral lo ... | 2010 | 20074645 |
| the complete mitochondrial genomes of the yellowleg shrimp farfantepenaeus californiensis and the blue shrimp litopenaeus stylirostris (crustacea: decapoda). | mitochondria play key roles in many cellular processes. description of penaeid shrimp genes, including mitochondrial genomes are fairly recent and some are focusing on commercially important shrimp as the pacific shrimp litopenaeus vannamei that is being used for aquaculture not only in america, but also in asia. much less is known about other pacific shrimp such as the yellowleg shrimp farfantepenaeus californiensis and the blue shrimp litopenaeus stylirostris. we report the complete mitogenome ... | 2009 | 20403743 |
| metal and metalloid bioaccumulation in the pacific blue shrimp litopenaeus stylirostris (stimpson) from new caledonia: laboratory and field studies. | the present work aimed at better understanding metal and metalloid bioaccumulation in the edible pacific blue shrimp litopenaeus stylirostris, using both laboratory and field approaches. in the laboratory, the bioaccumulation kinetics of ag, cd, co, cr, and zn have been investigated in shrimp exposed via seawater and food, using the corresponding gamma-emitting radiotracers ((110 m)ag, (109)cd, (57)co, (51)cr, and (65)zn) and highly sensitive nuclear detection techniques. results showed that hep ... | 2010 | 20637480 |
| structure of penaeus stylirostris densovirus, a shrimp pathogen. | penaeus stylirostris densovirus (pstdnv), a pathogen of penaeid shrimp, causes significant damage to farmed and wild shrimp populations. in contrast to other parvoviruses, pstdnv probably has only one type of capsid protein that lacks the phospholipase a2 activity that has been implicated as a requirement during parvoviral host cell infection. the structure of recombinant virus-like particles, composed of 60 copies of the 37.5-kda coat protein, the smallest parvoviral capsid protein reported thu ... | 2010 | 20702621 |
| development of a loop-mediated isothermal amplification assay for rapid and sensitive detection of ostreid herpesvirus 1 dna. | a loop-mediated isothermal amplification (lamp) assay was developed for rapid, specific and sensitive detection of ostreid herpesvirus 1 (oshv-1) dna. a set of four primers was designed, based on the sequence of the atpase subunit of the oshv-1 dna-packaging terminase gene. the reaction temperature and time were optimized to 64°c and 60min, respectively. lamp products were detected by agarose gel electrophoresis or by visual inspection of a color change due to addition of fluorescent dye. the de ... | 2010 | 20813133 |
| virulence of an emerging pathogenic lineage of vibrio nigripulchritudo is dependent on two plasmids. | vibrioses are the predominant bacterial infections in marine shrimp farms. vibrio nigripulchritudo is an emerging pathogen of the cultured shrimp litopenaeus stylirostris in new caledonia and other regions in the indo-pacific. the molecular determinants of v. nigripulchritudo pathogenicity are unknown; however, molecular epidemiological studies have revealed that recent pathogenic v. nigripulchritudo isolates from new caledonia all cluster into a monophyletic clade and contain a small plasmid, p ... | 2011 | 20825454 |
| double-stranded rna confers both preventive and therapeutic effects against penaeus stylirostris densovirus (pstdnv) in litopenaeus vannamei. | penaeus stylirostris densovirus (pstdnv) infection is found widespread in peneaid shrimp, especially in economically important species such as black tiger shrimp penaeus monodon and pacific white shrimp litopenaeus vannamei. although effective prevention method for viral diseases is not well established in shrimp, the treatment with viral specific double-stranded rna (dsrna) or sirna has given promising results. in present study, dsrnas corresponding to non-structural (orf1 and orf2 overlapping ... | 2010 | 20869997 |
| rapid and sensitive detection of infectious hypodermal and hematopoietic necrosis virus by loop-mediated isothermal amplification combined with a lateral flow dipstick. | infectious hypodermal and hematopoietic necrosis virus (ihhnv) is an important shrimp pathogen that causes mortality in penaeus stylirostris and stunting (called runt deformity syndrome or rds) in penaeus vannamei. loop-mediated isothermal amplification (lamp) allows rapid amplification of nucleic acids under isothermal conditions. it can be combined with a chromatographic lateral flow dipstick (lfd) for highly specific, rapid and simple visual detection of ihhnv-specific amplicons. using this p ... | 2010 | 20887752 |
| detection of infectious myonecrosis virus using monoclonal antibody specific to n and c fragments of the capsid protein expressed heterologously. | the gene encoding the capsid protein in orf1 of the genome of infectious myonecrosis virus (imnv) (genbank ay570982) was amplified into three parts named cp-n (nucleotides 2248-3045), cp-i (nucleotides 3046-3954) and cp-c (nucleotides 3955-4953). the cp-n fragment was inserted into expression vector ptyb1 while cp-i and cp-c were each inserted into expression vector pgex-6p-1 for transformation of bl21 e. coli strain. after induction, intein-cp-n (84 kda), glutathione-s-transferase (gst)-cp-i (6 ... | 2010 | 21029750 |
| complete nucleic acid sequence of penaeus stylirostris densovirus (pstdnv) from india. | infectious hypodermal and hematopoietic necrosis virus (ihhnv) of shrimp, recently been classified as penaeus stylirostris densovirus (pstdnv). the complete nucleic acid sequence of pstdnv from india was obtained by cloning and sequencing of different dna fragment of the virus. the genome organisation of pstdnv revealed that there were three major coding domains: a left orf (ns1) of 2001 bp, a mid orf (ns2) of 1092 bp and a right orf (vp) of 990 bp. the complete genome and amino acid sequences o ... | 2011 | 21402111 |
| shrimp laminin receptor binds with capsid proteins of two additional shrimp rna viruses yhv and imnv. | laminin receptor (lamr) in shrimp was previously proposed to be a potential receptor protein for taura syndrome virus (tsv) based on yeast two-hybrid assays. since shrimp lamr bound to the vp1 capsid protein of tsv, we were interested to know whether capsid/envelope proteins from other shrimp viruses would also bind to lamr. thus, capsid/envelope encoding genes from 5 additional shrimp viruses were examined. these were penaeus stylirostris densovirus (pstdnv), white spot syndrome virus (wssv), i ... | 2011 | 21414409 |
| additional random, single to multiple genome fragments of penaeus stylirostris densovirus in the giant tiger shrimp genome have implications for viral disease diagnosis. | scattered reports of viral inserts in shrimp and insect genomes led to the hypothesis that random, autonomous insertion of such sequences occurs in these organisms and leads to specific, heritable immunity. to test the prediction regarding random insertion of viral sequences into the shrimp genome, we examined the giant tiger shrimp for random genomic insertions of penaeus stylirostris densovirus (also called ihhnv). by pcr analysis using a set of 7 overlapping primer pairs to cover the whole ih ... | 2011 | 21723348 |
| Multiplex real-time PCR and high-resolution melting analysis for detection of white spot syndrome virus, yellow-head virus, and Penaeus monodon densovirus in penaeid shrimp. | A multiplex real-time PCR and high-resolution melting (HRM) analysis was developed to detect simultaneously three of the major viruses of penaeid shrimp including white spot syndrome virus (WSSV), yellow-head virus (YHV), and Penaeus monodon densovirus (PmDNV). Plasmids containing DNA/cDNA fragments of WSSV and YHV, and genomic DNAs of PmDNV and normal shrimp were used to test sensitivity of the procedure. Without the need of any probe, the products were identified by HRM analysis after real-tim ... | 2011 | 21906627 |
| cdna cloning, characterization and expression analysis of a novel antimicrobial peptide gene penaeidin-3 (fi-pen3) from the haemocytes of indian white shrimp fenneropenaeus indicus. | a new member of antimicrobial peptide genes of the penaeidin family, penaeidin 3, was cloned from the haemocytes of indian white shrimp fenneropeneaus indicus (f. indicus), by reverse transcription pcr (rt-pcr) and rapid amplification of cdna end (race-pcr) methods. the complete nucleotide sequence of cdna clone of indian white shrimp f. indicus penaeidin 3 (fi-pen3) was 243bp long and has an open reading frame which encodes 80 amino acid peptide. the homology analysis of fi-pen3 sequence with o ... | 2011 | 21885268 |
| genomics, molecular epidemiology and diagnostics of infectious hypodermal and hematopoietic necrosis virus. | infectious hypodermal and hematopoietic necrosis virus (ihhnv) is one of the major viral pathogens of penaeid shrimps worldwide, which has resulted in severe mortalities of up to 90 % in cultured penaeus (litopenaeus) stylirostris from hawaii and hence designated penaeus stylirostris densovirus (pstdnv). ihhnv is distributed in shrimp culture facilities worldwide. it causes large economic loss to the shrimp farming industry. our knowledge about the natural reservoirs of ihhnv is still scarce. re ... | 2012 | 23997444 |
| immunological-based assays for specific detection of shrimp viruses. | among shrimp viral pathogens, white spot syndrome virus (wssv) and yellow head virus (yhv) are the most lethal agents, causing serious problems for both the whiteleg shrimp, penaeus (litopenaeus) vannamei, and the black tiger shrimp, penaeus (penaeus) monodon. another important virus that infects p. vannamei is infectious myonecrosis virus (imnv), which induces the white discoloration of affected muscle. in the cases of taura syndrome virus and penaeus stylirostris densovirus (pstdnv; formerly k ... | 2014 | 24567913 |
| development of duplex real-time pcr for the detection of wssv and pstdv1 in cultivated shrimp. | the white spot syndrome virus (wssv) and penaeus stylirostris penstyldensovirus 1 (previously named infectious hypodermal and hematopoietic necrosis virus-ihhnv) are two of the most important viral pathogens of penaeid shrimp. different methods have been applied for diagnosis of these viruses, including real-time pcr (qpcr) assays. a duplex qpcr method allows the simultaneous detection of two viruses in the same sample, which is more cost-effective than assaying for each virus separately. curren ... | 2014 | 24996437 |
| persistence of penaeus stylirostris densovirus delays mortality caused by white spot syndrome virus infection in black tiger shrimp (penaeus monodon). | persistent infection of penaeus stylirostris densovirus (pstdnv) (also called ihhnv) and its non-infectious inserts in the black tiger shrimp, penaeus monodon (p. monodon) genome are commonly found without apparent disease. here, we introduced the method of multiplex pcr in order to differentiate shrimp with viral inserts from ones with the infectious virus. the method allowed us to study the effect of pre-infection of ihhnv, in comparison to ihhnv inserts, on wssv resistance in p. monodon. | 2013 | 23414329 |
| an improved validated sybr green-based real-time quantitative pcr assay for the detection of the penaeus stylirostris densovirus in penaeid shrimp. | the penaeus stylirostris densovirus (pstdv) (also known as infectious hypodermal and hematopoietic necrosis virus, ihhnv), one of the major shrimp pathogens, has a worldwide distribution in farmed and wild shrimp populations. outbreaks of ihhnv have been associated with substantial economic losses which are accompanied by a negative social impact. current diagnostic pcr tests may result in false-positive results as several parts of pstdv genome may be endogenized in the nuclear genome of the shr ... | 2015 | 25445798 |
| prevalence of the infectious hypodermal and hematopoietic necrosis virus in shrimp (penaeus vannamei) broodstock in northwestern mexico. | the penaeus stylirostris densovirus (pstdnv or ihhnv) is the smallest of the known shrimp viruses. it causes severe mortalities in juveniles and sub-adults of the blue shrimp penaeus stylirostris, while specimens of the white shrimp penaeus vannamei infected by this virus exhibit reduced growth rates and negative effects on the feed-conversion rate (fcr). to date, no descriptive epidemiological surveys on the prevalence of this virus in shrimp broodstock have been performed. in this study, the p ... | 2014 | 25287323 |
| biology, genome organization, and evolution of parvoviruses in marine shrimp. | as shrimp aquaculture has evolved from a subsistent farming activity to an economically important global industry, viral diseases have also become a serious threat to the sustainable growth and productivity of this industry. parvoviruses represent an economically important group of viruses that has greatly affected shrimp aquaculture. in the early 1980s, an outbreak of a shrimp parvovirus, infectious hypodermal and hematopoietic necrosis virus (ihhnv), led to the collapse of penaeid shrimp farmi ... | 2014 | 24751195 |
| prevalence, diversity and co-occurrence of the white spot syndrome virus, monodon baculovirus and penaeus stylirostris densovirus in wild populations of penaeus monodon in the philippines. | the farming of the black tiger shrimp penaeus monodon in the philippines relies on wild broodstock. pcr was thus used to determine the prevalence of white spot syndrome virus (wssv), monodon baculovirus (mbv) and penaeus stylirostris densovirus (pstdv) in a total of 178 shrimp from 6 geographically disparate locations where broodstock are captured for use in hatcheries. pcr amplicons were also sequenced to identify phylogenetic relationships of the virus haplotypes detected. shrimp from southeas ... | 2017 | 28792418 |