| wasalexins a and b, new phytoalexins from wasabi: isolation, synthesis, and antifungal activity. | the chemical structure determination of two phytoalexins from wasabi (wasabia japonica, syn. eutrema wasabi), a plant resistant to virulent isolates of the blackleg fungus [leptosphaeria maculans (desm.) ces. et de not., asexual stage phoma lingam (tode ex fr.) desm.], as well as their synthesis and antifungal activity towards isolates of p. lingam and p. wasabiae is reported. | 1999 | 10571166 |
| phomalairdenone: a new host-selective phytotoxin from a virulent type of the blackleg fungus phoma lingam. | the chemical structure and bioactivity of phomalairdenone (7), a new sesquiterpenic host-selective phytotoxin produced by an unusual virulent type isolate of the blackleg fungus [phoma lingam, perfect stage leptosphaeria maculans (desm.) ces. et de not.] are reported. | 1999 | 10612587 |
| hplc analyses of cultures of phoma spp.: differentiation among groups and species through secondary metabolite profiles. | the metabolite profiles of 26 isolates of the blackleg fungus (leptosphaeria maculans (desm.) ces. et de not., asexual stage phoma lingam (tode ex fr.) desm.), obtained from diverse parts of the world (part of the international blackleg crucifer network collection), were studied utilizing specific culture conditions, hplc analysis, and a set of chemical markers. this fungus is the causative agent of blackleg disease of brassica oilseeds; a virulent strain of the pathogen has caused significant r ... | 2000 | 10941513 |
| strain typing of polish leptosphaeria maculans isolates supports at the genomic level the multi-species concept of aggressive and non-aggressive strains. | 47 polish isolates of the blackleg fungus leptosphaeria maculans (phoma lingam) were compared with eight well-defined reference strains from germany, france, denmark, australia and one polish isolate of phoma nigrificans. the isolates were tested (i) for growth characteristics, (ii) for their ability to form sirodesmins, (iii) for cellulolytic enzymes, and (iv) for pathotype-differentiating molecular markers generated by rapd-pcr, pcr analysis with pathotype-specific primer pairs and pfge. with ... | 2001 | 11572457 |
| probing the phytopathogenic blackleg fungus with a phytoalexin homolog. | | 1998 | 11672020 |
| isolation, structure determination, and phytotoxicity of unusual dioxopiperazines from the phytopathogenic fungus phoma lingam. | the isolation, chemical structure elucidation, and bioactivity of polanrazines b-f, five new dioxopiperazines produced by isolates of the blackleg fungus [phoma lingam, perfect stage leptosphaeria maculans (desm.) ces. et de not.] originating from poland, are reported. polanrazines c and e showed moderate but selective toxicity, causing necrotic and chlorotic lesions (1-3 mm diameter) on brown mustard leaves. | 2001 | 11684188 |
| production of 2,5-dioxopiperazine by a new isolate type of the blackleg fungus phoma lingam. | new isolates of the blackleg fungus [phoma lingam, perfect stage leptosphaeria maculans (desm.) ces. et de not.], virulent to the traditionally resistant brown mustard (brassica juncea), produce l-valyl-l-tryptophan anhydride, a 2,5-dioxopiperazine different from those produced by blackleg isolates virulent to canola (b. napus and b. rapa). | 1998 | 11711067 |
| chemical defenses of crucifers: elicitation and metabolism of phytoalexins and indole-3-acetonitrile in brown mustard and turnip. | the metabolism of the cruciferous phytoalexins brassinin and cyclobrassinin, and the related compounds indole-3-carboxaldehyde, glucobrassicin, and indole-3-acetaldoxime was investigated in various plant tissues of brassica juncea and b. rapa. metabolic studies with brassinin showed that stems of b. juncea metabolized radiolabeled brassinin to indole-3-acetic acid, via indole-3-carboxaldehyde, a detoxification pathway similar to that followed by the "blackleg" fungus (phoma lingam/leptosphaeria ... | 2002 | 11867093 |
| new sesquiterpenic phytotoxins establish unprecedented relationship between different groups of blackleg fungal isolates. | a comprehensive search for sesquiterpenic metabolites produced by isolates of the blackleg fungus [leptosphaeria maculans (desm.)] ces. et de not. [asexual stage phoma lingam (tode ex fr.) desm.] revealed that an isolate pathogenic on both canola and brown mustard (ibcn 18) and two isolates pathogenic on brown mustard (laird 2 and mayfair 2) produced similar sesquiterpenes. the isolation, chemical structure elucidation, and phytotoxicity of these new sesquiterpenes with silphinene and selinene t ... | 2005 | 15755649 |
| rga- and rapd-derived scar markers for a brassica b-genome introgression conferring resistance to blackleg in oilseed rape. | an introgression derived from the b genome of brassica juncea in spring-type oilseed rape (b. napus) conferring recessively inherited cotyledon resistance against several pathotypes of the blackleg fungus leptosphaeria maculans was mapped using pcr-based molecular markers. resistance-associated b-genome-specific randomly amplified (rapd) and resistance gene analog (rga) dna polymorphisms were converted into three sequence-specific markers (scars; b5-1520, c5-1000, rgalm). the flanking sequence o ... | 2005 | 15887037 |
| complexities of chromosome landing in a highly duplicated genome: toward map-based cloning of a gene controlling blackleg resistance in brassica napus. | the lmr1 locus, which controls seedling resistance to the blackleg fungus leptosphaeria maculans in the brassica napus cultivar shiralee, was positioned on linkage group n7. fine genetic mapping in a population of 2500 backcross lines identified three molecular markers that cosegregated with lmr1. additional linkage mapping in a second population colocalized a seedling resistance gene, clmr1, from the cultivar cresor to the same genetic interval on n7 as lmr1. both genes were located in a region ... | 2005 | 16143600 |
| metabolism of the crucifer phytoalexins wasalexin a and b in the plant pathogenic fungus leptosphaeria maculans. | wasalexins a and b are crucifer phytoalexins produced by two substantially different plant species, a wild species abundant in the canadian prairies and a condiment plant widely cultivated in japan. interestingly, both plant species are resistant to an economically important fungal plant pathogen, the blackleg fungus [leptosphaeria maculans (desm.) ces. et de not., asexual stage phoma lingam (tode ex fr.) desm.]. the transformation of wasalexins a and b in cultures of isolates of l. maculans, an ... | 2006 | 17036150 |
| are pectins involved in cold acclimation and de-acclimation of winter oil-seed rape plants? | the hypothesis was tested that pectin content and methylation degree participate in regulation of cell wall mechanical properties and in this way may affect tissue growth and freezing resistance over the course of plant cold acclimation and de-acclimation. | 2008 | 18222909 |
| molecular mapping of resistance to leptosphaeria maculans in australian cultivars of brassica napus. | doubled haploid (dh) lines together with a cotyledon bioassay were employed for the molecular analysis of resistance to the blackleg fungus leptosphaeria maculans in the australian brassica napus cultivars shiralee and maluka. we used bulked segregant analysis to identify 13 rapd and two rflp markers linked to the resistance phenotype and mapped these markers in the segregating dh population. our data suggest the presence of a single major locus controlling resistance in the cultivar shiralee, c ... | 1997 | 18464830 |
| epidemiology of blackleg (leptosphaeria maculans) of canola (brassica napus) in relation to maturation of pseudothecia and discharge of ascospores in western australia. | abstract the timing of maturation of pseudothecia and discharge of ascospores of the blackleg fungus (leptosphaeria maculans) is critical in relation to infection early in the cropping season of canola. during 1998 to 2000, development of pseudothecia was investigated on residues of the previous year's canola crop collected from four agroclimatically different locations: mount barker (southern high rainfall), wongan hills (central medium rainfall), merredin (central low rainfall), and east chapm ... | 2007 | 18943642 |
| the stem canker (blackleg) fungus, leptosphaeria maculans, enters the genomic era. | summary leptosphaeria maculans is the most ubiquitous pathogen of brassica crops, and mainly oilseed brassicas (oilseed rape, canola), causing the devastating 'stem canker' or 'blackleg'. this review summarizes our current knowledge on the pathogen, from taxonomic issues to specific life traits. it mainly illustrates the importance of formal genetics approaches on the pathogen side to dissect the interaction with the host plants. in addition, this review presents the main current research topics ... | 2005 | 20565653 |
| impact of cruciferous phytoalexins on the detoxification of brassilexin by the blackleg fungus pathogenic to brown mustard. | the biotransformation of brassilexin, a potent phytoalexin produced by brown mustard (brassica juncea l.), in the presence of various cruciferous phytoalexins was investigated. an important group of isolates of the fungal species leptosphaeria maculans (laird 2 and mayfair 2), which is virulent to brown mustard, but not to canola, was used in this investigation. brassilexin was detoxified by the fungus, but none of the phytoalexins seemed to affect substantially the rate of brassilexin detoxific ... | 2010 | 20614815 |
| evolution of linked avirulence effectors in leptosphaeria maculans is affected by genomic environment and exposure to resistance genes in host plants. | brassica napus (canola) cultivars and isolates of the blackleg fungus, leptosphaeria maculans interact in a 'gene for gene' manner whereby plant resistance (r) genes are complementary to pathogen avirulence (avr) genes. avirulence genes encode proteins that belong to a class of pathogen molecules known as effectors, which includes small secreted proteins that play a role in disease. in australia in 2003 canola cultivars with the rlm1 resistance gene suffered a breakdown of disease resistance, re ... | 2010 | 21079787 |
| molecular phylogeny of leptosphaeria and phaeosphaeria. | the objectives of this study were to determine the phylogenetic relationships of species of leptosphaeria and phaeosphaeria and evaluate the phylogenetic significance of morphological characters of the teleomorph, anamorph, and host. sequences of the entire its region, including the 5.8s rdna, of 59 isolates representing 54 species were analyzed and the phylogeny inferred using parsimony and distance analyses. isolates grouped into three well-supported clades. the results of this study support t ... | 2002 | 21156536 |
| saccharicola, a new genus for two leptosphaeria species on sugar cane. | leptosphaeria bicolor, causal agent of a leaf scorch disease of sugar cane, is referred to the new genus saccharicola. the ascospores are 1-3 transseptate and hyaline at first but become melanized and rough after release, as is the case in some members of massarina and lophiostoma. ssu rdna data indicate that it is closely related to m. eburnea but is biotrophic in leaves of sugar cane and not corticolous, the ascomata are less melanized, and it has stagonospora- and phoma-like synanamorphs, not ... | 2003 | 21156631 |