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nitrogen fixation, denitrification, and pleomorphic growth in a highly pigmented spirillum lipoferum.a strain of spirillum lipoferum with intense red pigmentation was isolated from the roots of cynodon dactylon "coastal." this isolate vigorously reduced acetylene when grown in n-free, na-malate, semisolid agar, and it was identical to s. lipoferum strain sp7 by standard taxonomic tests. both s. lipoferum sp7 and the c. dactylon root isolate displayed the unique features of being denitrifiers as well as n2 fixers. the n2-dependent growth curve was biphasic: cells in younger cultures showed the c ...197722311
hypoglycemic effect of plants used in mexico as antidiabetics.the objective of this work is to investigate the hypoglycemic effect of 12 "antidiabetic" plants used in mexico. the studies were performed using 27 healthy rabbits with the gastric administration of water, tolbutamide or decoction of the "antidiabetic" plant before the induction of temporary hyperglycemia by subcutaneous injection of 50% dextrose solution (4 ml/kg of weight) at the beginning of the experiment and after 60 min. blood glucose was determined every 60 min for a period of 5 h. tolbu ...19921308793
diet characteristics, digesta kinetics, and dry matter intake of steers grazing eastern gamagrass.eastern gamagrass (tripsacum dactyloides [l.] l.) has attracted attention as a forage crop, but information on its use is lacking. this 2-yr study compared diet quality, ingestive mastication, and adg by steers grazing eastern gamagrass (gg), flaccidgrass (pennisetum flaccidum griseb.), and tifton 44 bermudagrass (cynodon dactylon [l.] pers.). the design was a randomized complete block with two agronomic replicates. the diet selected by steers from gg in may did not differ from the diet selected ...19921316349
implant and copper oxide needles for steers grazing acremonium coenophialum-infected tall fescue pastures: effects on grazing and subsequent feedlot performance and serum constituents.two experiments were conducted to compare the effects of a progesterone-estradiol implant (pei) with no implant (ni) and 20 g of copper oxide needles (cuon) with no cuon on grazing, subsequent feedlot performance, and selected serum constituents of steers. in exp. 1, 114 limousin crossbred yearling steers (317 kg average initial bw) were stocked continuously on acremonium coenophialum-infected tall fescue (festuca arundinacea schreb.)-ladino clover (trifolium repens l.) pastures (c) or were rota ...19921429296
technical note: stocking equivalents and stocking rate-gain relationships for steers and cow-calf pairs grazing oversown bermuda grass.grazing experiments may use steers or cow-calf pairs for measuring animal performance on pasture treatments, but the validity of extrapolation between these classes of cattle has not been verified. a grazing study was conducted in the spring and summer of both 1988 and 1989 to determine stocking equivalents and stocking rate-weight gain relationships for steers and cow-calf pairs grazing coastal bermuda grass (cynodon dactylon [l.] pers.) oversown with rye (secale cereale l.) and ryegrass (loliu ...19921429299
purification and characterization of an allergen from celery immunochemically related to an allergen present in several other plant species. identification as a profilin.the purification of a 15 kd allergen from celery was obtained by a four step procedure. evidence for at least two isoallergenic forms was obtained after analysis by two-dimensional-electrophoresis. a rabbit polyclonal antibody raised against this purified allergen allowed us to confirm our precedent results on the occurrence of allergenically and molecular mass-related components in celery and birch and mugwort pollens. in addition such components were also present in numerous other species like ...19921525697
olive (olea europea) and privet (ligustrum vulgare) pollen allergens. identification and cross-reactivity with grass pollen proteins.protein blotting studies showed that three olive pollen components with mol. wts approximately 18-19, 20 and 40 kd can be considered to be major allergens. for privet pollen, the highest recognition frequencies were for allergens of mol. wts approximately 20, approximately 19, approximately 40 and approximately 70 kd. when results with the 62 subjects examined were separated into groups corresponding to their geographical locations, viz. italy, france and australia, subjects sensitized to olive, ...19921528191
monitoring and assessment of mercury pollution in the vicinity of a chloralkali plant. iv. bioconcentration of mercury in in situ aquatic and terrestrial plants at ganjam, india.in situ aquatic and terrestrial plants including a few vegetable and crop plants growing in and around a chloralkali plant at ganjam, india were analyzed for concentrations of root and shoot mercury. the aquatic plants found to bioconcentrate mercury to different degrees included marsilea spp., spirodela polyrhiza, jussiea repens, paspalum scrobiculatam, pistia stratiotes, eichhornia crassipes, hygrophila schulli, monochoria hastata and bacopa monniera. among wild terrestrial plants chloris barb ...19921536599
evaluation of the captec chrome controlled-release device for the estimation of fecal output by grazing sheep.three grazing experiments were conducted to compare actual fecal output (fo) by sheep, determined with fecal collection bags, to fecal output predicted using captec chromic oxide (cr2o3) controlled-release capsules. in exp. 1, 14 crossbred wethers dosed with controlled-release cr2o3 capsules rotationally grazed four paddocks of alfalfa (medicago sativa l.) for 18 d. total fecal collection and grab samples were taken daily from each animal; feces were dried and assayed for cr. from d 5 to 12 afte ...19921644698
effects of grass species on grazing steers: i. diet composition and ingestive mastication.in traditional grazing trials, per animal and per hectare productivity are determined, but pasture and animal measurements are generally inadequate to address reasons for different treatment responses. this 2-yr study examined the diet and diet characteristics of steers grazing tall fescue (festuca arundinacea schreb.), switchgrass (panicum virgatum l.), flaccidgrass (pennisetum flaccidum griseb.), and bermudagrass (cynodon dactylon [l.] pers.) pastures. a randomized complete block design was us ...19911648065
effect of ammonium carbamate on nutritive and preservative characteristics of high-moisture coastal bermudagrass hay.two studies evaluated the effects of ammonium carbamate (ac) on preservation and digestibility of high-moisture (hm) coastal bermudagrass (cynodon dactylon) hay. a 3 x 7 factorial arrangement of treatments in a completely random design was used to estimate the efficacy of ac in laboratory conditions. treatments were 25, 30, or 35% moisture (m) forage treated with levels (l) of 0, 1.14, 2.28, 3.42, 4.56, 5.70, or 6.84% ac. nitrogen in forage increased (p less than .01) linearly due to m and l. th ...19911653198
analysis of allergenic components of bermuda grass pollen by monoclonal antibodies.a panel of 16 monoclonal antibodies (moabs) directed against bermuda grass (cynodon dactylon) pollen (bgp) were generated for identification and purification of the major allergenic components of the eliciting antigen (ag). radioimmunoprecipitation (rip) analysis revealed that there were at least eight antigenic components with molecular weights (mw) ranging from 12 kilodalton (12 kda) to 200 kda. each of these components has distinct biochemical characteristics based on sodium dodecyl sulfate-p ...19911796777
characterization of the major allergen of cynodon dactylon (bermuda grass) pollen, cyn d i.an allergen from cynodon dactylon (bermuda grass) pollen, cyn d i, has been purified by a combination of concanavalin a-sepharose affinity chromatography, and carboxymethyl-sepharose chromatography. the allergen constitutes the major allergenic component of the pollen extract as observed by immunoelectrophoretic techniques, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, an ige-inhibition experiment, and skin testing. in sodium dodecyl sulfate-polyacrylamide gel electrophoresis, cyn d ...19911955635
effects of grass species on grazing steers: ii. dry matter intake and digesta kinetics.animal responses to treatments in grazing experiments frequently remain unexplained because of inadequate pasture and(or) animal measurements. this 2-yr study examined dmi, gastrointestinal tract fill of undigested dm (fill), rate of digesta passage (rop), and digesta mean retention time (mrt) for steers grazing tall fescue (festuca arundinacea schreb.), switchgrass (panicum virgatum l.), flaccidgrass (pennisetum flaccidum griseb.), and bermudagrass (cynodon dactylon [l.] pers.). a randomized co ...19912061249
the flow of undigested corn residues through the gastrointestinal tract of cattle.two experiments were conducted to measure effects of source and level of roughage on the flow of corn residues through the gastrointestinal tract of cattle. in exp. 1, steers (195 kg) were fed diets of ground corn with 0, 30 or 60% of ground coastal bermudagrass hay (cynodon dactylon) [l.] pers.) at intakes of 1, 1.5 or 2% of bw in a 9 x 9 latin square. experiment 2 consisted of two 4 x 4 latin squares with either rice hulls (square 1) or ground coastal bermudagrass hay (square 2) providing 0, 7 ...19902175746
ruminal release of minerals from six forage species.an in vitro technique and an in situ technique were used to measure release of ca, mg, p and zn over time from hand-clipped alfalfa (medicago sativa l.). maximal release of p and zn was greater using the in situ technique. 'florida 77' alfalfa (medicago sativa l.), 'florigraze' rhizoma peanut (arachis glabrata benth.), 'mott' dwarf elephantgrass (pennisetum purpureum schum.), 'tifton 78' bermudagrass (cynodon dactylon (l) pers.), 'pensacola' bahiagrass (paspalum notatum flugge) and 'floralta' li ...19902384396
eating and ruminating behavior of steers fed coastal bermudagrass hay at four levels.effects of feeding level on chewing behavior and physical reduction of particles during ingestive mastication and rumination were examined in four ruminally cannulated steers fed coastal bermudagrass (cynodon dactylon [l.] pers.) hay in the long form. the experimental design was a 4 x 4 latin square with 18-d periods. treatments based on previous intakes were set at 50, 70, 90 and 110% of feed consumed per animal. steers were fed at 12-h intervals. chewing behavior was monitored over 4 d with a ...19892613586
effects of ingestive mastication on particle dimensions and weight distribution of coastal bermudagrass hay fed to steers at four levels.coastal bermudagrass (cynodon dactylon [l.] pers.) hay was fed in the long form to four ruminally cannulated steers to evaluate the effects of intake level on the physical reduction of feed particles during ingestive mastication. the experimental design was a 4 x 4 latin square with 18-d periods. treatments based on previous intakes were set at 50, 70, 90 and 110% of feed consumed per animal and fed at 12-h intervals. boli of ingested, masticated hay were collected at the cardia and separated by ...19892703448
an immunoelectrophoretic analysis of the allergens of cynodon dactylon (bermuda grass) pollen.a crude extract of cynodon dactylon (bermuda grass) pollen was obtained by extraction, centrifugation, dialysis, and lyophilization. the corresponding polyspecific rabbit antibodies were obtained by immunization, bleeding, and purification and were used for a cross immunoelectrophoretic analysis of the crude extract. at least 52 antigens (ags), of which 47 migrated toward the anode, and five, toward the cathode, were revealed. crossed radioimmunoelectrophoretic analysis performed with sera from ...19892732412
identification of allergens and antigens of bermuda grass (cynodon dactylon) pollen by immunoblot analysis.allergens and antigens of bermuda grass pollen fractionated by sds-polyacrylamide gel electrophoresis and transferred to nitrocellulose membranes were identified using twenty-one sera of bermuda grass pollen-allergic patients. the ige- and igg-binding pollen components transferred to nitrocellulose were detected by reaction with enzyme-labelled anti-human ige and anti-human igg, respectively. there was heterogeneity in both ige- and igg-binding patterns of the allergic sera tested. fourteen poll ...19883046777
recognition of pollen and other particulate aeroantigens by immunoblot microscopy.most grass-pollen types appear identical by normal light microscopy. restricted antigenic cross-reactivity of cynodon dactylon (bermuda grass) pollen allowed development of a new method to identify antigens associated with grass-pollen grains. pollen applied to the surface of an adhesive tape was blotted onto nitrocellulose, and the blots were identified by anti-bermuda-grass antibodies, second antibody, and fluorescence microscopy. of the 44 species of grass pollen studied for specificity of th ...19883049747
selection among nonlinear models for rate of passage studies in ruminants.eight toggenburg wether goats were fed either coastal bermudagrass (cynodon dactylon) or orchardgrass (dactylis glomerata)-alfalfa (medicago sativa) hay (57:43%) in a reversal design. four markers were used to study the movement of different digesta fractions: erbium for large particles greater than 1,700 microns; ytterbium for medium particles less than 1,700 microns and greater than 500 microns; chromium for small particles less than 500 microns and greater than 32 microns; and cobalt to follo ...19883225248
degradation of polysaccharides and lignin by ruminal bacteria and fungi.bermudagrass (cynodon dactylon) leaf blades and whole cordgrass (spartina alterniflora) fiber were evaluated for degradation of cell walls by microbial groups in ruminal fluid. the groups were selected by the addition of antibiotics to the inoculum as follows: (i) whole ruminal fluid (wrf), no antibiotics; (ii) cycloheximide (c) to inhibit fungi, thus showing potential bacterial activity; (iii) streptomycin and penicillin (s,p) to inhibit fiber-degrading bacteria, showing potential fungal activi ...19883389808
rumen fungi: morphological types from georgia cattle and the attack on forage cell walls.fungal colonies developing in anaerobic media from zoospores in rumen fluid from cows eating cynodon dactylon or medicago sativa included types showing monocentric and polycentric growth. high energy supplements added to diets of sorghum bicolor silage increased fungal numbers in the rumen, but increases were also affected by the history and predisposition of the animal. mixed fungal types in rumen fluid and pure cultures of isolates showing monocentric and polycentric growth degraded and weaken ...19883395692
rast-specific ige in nigerian asthmatic patients.this first study of the pattern of allergen-specific ige in nigerian asthmatic patients in zaria shows a significant percentage with specific ige to dermatophagoides pteronnyssinus, aspergillus fumigatus, and cynodon dactylon (bermuda grass). total serum ige varied in both urban and rural asthmatics with a pattern of low and high levels in all groups.19863484920
the occurrence of indoor allergens in saudi arabia.investigations on indoor airborne allergens in saudi arabia were performed by mold cultures and dust analyses by counter-current immunoelectrophoresis. twenty fungal genera were isolated, with aspergillus as the most often encountered. most of the dust-bound fungi found are ubiquitous and common. antibodies against dermatophagoides pteronyssinus, cat- cow- and rat dander, and cynodon dactylon pollen were used in the dust analyses. animal antigens were found in five of the ten dust samples. house ...19854014781
[use of sugar cane harvest residues in cattle fattening in the republic of cuba. 1. the physical and chemical composition of the residues and the effect of naoh treatment on their in vitro and in vivo digestibility].the harvesting remains of sugar cane (crowns, leaves, sheaths, parts of stalks), which are rich in crude fibre, were investigated. the content of crude nutrients, their digestibility and the energy concentration of the harvesting remains largely correspond to the values of cereal straw. after the treatment with growing amounts of naoh (0, 4, 6, 8, 10 g/ 100 g dm) a significantly increasing digestibility of the dry matter (31.9, 47.0, 56.1, 61.9, 67.6%) was ascertained in in-vitro experiments. in ...19854096638
an increase in nitrogen content of setaria italica and zea mays inoculated with azospirillum.bacteria belonging to the genus azospirillum isolated from cynodon dactylon roots in israel were compared with azospirillum brasilense from brazil and california for their ability to fix nitrogen in association with grasses under greenhouse conditions. the plants were grown in a system which avoided cross inoculation from the inoculated soil to the control, while maintaining the natural soil microflora and humidity level in the soil close to field capacity. the organisms tested significantly inc ...19806769575
lysine supplementation of diets for yearling horses.forty-six thoroughbred and quarter horse yearlings were used in two experiments designed to determine the amount of lysine in the concentrate necessary to promote maximum growth when fed the coastal bermudagrass (cynodon dactylon) hay provided at 1% body weight (bw)/day. in the first experiment, a 196-day trial, supplemental protein sources and analyses of the concentrates compared were: (1) soybean meal (sbm; 15.4% crude protein, .70% lysine); (2) sbm + .2% lysine (15.7% crude protein, .81% lys ...19816804430
anna pavala sindhooram--an antiatherosclerotic indian drug.the procedure for the preparation of anna pavala sindhooram (aps), a drug based on the concepts of indian medicine for the prevention and reversal of the atherosclerotic disease process is described in detail. the uniformity in the composition of samples of the drug obtained in 10 batches, prepared over a period of 5 years is evident from the chemical analysis of the mineral constituents. the possibility of iodine, copper, iron, calcium and magnesium present in the anna pavala sindhooram, acting ...19836876850
comparative studies of nitrogen-fixing bacteria associated with grasses in israel with azospirillum brasilense.free-living, dinitrogen-fixing bacteria associated with roots of grasses were isolated from several locations in israel. bacteria with characteristics similar to those of azospirillum were isolated from cynodon dactylon roots and were compared with azospirillum brasilense from brasil (sp-7) and from california (cd). colonies of the israeli isolates were yellow and consisted of curved rods, 0.5-0.6 micron in diameter with polar flagella, whereas colonies of a. brasilense were pink (sp-7) and red ...19807397611
the effect of ascorbate on cellular humoral immunity in asthmatic children.ten white children with bronchial asthma and exercise-induced bronchoconstriction were assessed immunologically before and 1, 3 and 6 months after the commencement of standard therapy supplemented with ascorbate 1 g/d. the tests of cellular immune function were neutrophil chemotaxis, phagocytosis and resting and stimulated nitroblue tetrazolium reduction, and lymphocyte mitogen-induced transformation. humoral functions measured were secretory iga, serum immunoglobulins, alpha 1-antitrypsin, c3, ...19807444701
trace mineral supplementation of yearling horses.thirty-three thoroughbred and quarter horse yearlings were used in two experiments (18 in exp. 1 and 15 in exp. 2) to determine the influence of trace mineral (tm) supplementation on growth and bone mineral content in young growing horses from 340 to 452 d of age. in each experiment the yearlings were assigned at random within breed and sex outcome groups to one of three treatments. horses were fed assigned concentrates individually to appetite for two 1.5-h feeding periods, daily and group-fed ...19957601780
characterization of the isoforms of the group i allergen of cynodon dactylon.the group i allergen of cynodon dactylon, cyn d i, was found to consist of four to 10 isoforms.19957797789
effects of bermuda grass (cynodon dactylon cv. coast cross) and rice hulls on growth performance of 30 day-old weaned rabbits.several studies have demonstrated that rabbits can be maintained on diets containing high levels of crude fiber (cf) when compared to other monogastric animals. in the present study, we examined the effects of rice hulls and of bermuda grass (cv. coast cross) on the growing performance of 30 day-old weaned rabbits. rabbits were fed one of 5 diets containing rice hulls and/or bermuda grass as fiber source at the following proportions (bg/rh) 0/17.5, 15/11, 0/29, 14.7/19.1 and 48.5/0 for diets a, ...19937872830
dairy heifer and bermudagrass pasture responses to rotational and continuous stocking.during 1990 and 1991, pastures of 'callie' bermudagrass [cynodon dactylon (l.) pers.] located near gainesville, fl (29 degrees 60' n latitude) were used to determine the effects of three grazing methods on performance of 250-kg holstein heifers (bos taurus) and forage nutritive value and botanical composition. the grazing methods were rotational stocking (15 paddocks) with short grazing periods (1.5 to 2.5 d/paddock), rotational stocking (three paddocks) with long grazing periods (10 to 14 d/pad ...19948120191
whole-tract digesta kinetics and comparison of techniques for the estimation of fecal output in steers fed coastal bermudagrass hay at four levels of intake.coastal bermudagrass (cynodon dactylon [l.] pers) hay was fed to four ruminally cannulated steers (380 +/- 14 kg bw) of evaluate the effects of intake level on digesta flow kinetics in a 4 x 4 latin square design. forage intakes represented 50, 70, 88, and 99% of feed voluntarily consumed per animal during a pre-experimental period. masticated boli and wet-sieved masticated leaves (ml) and stems (ms) retained by a 4.0-mm sieve and feces retained by a .063-mm sieve were mordanted with chromium or ...19948138490
forage and splanchnic tissue mass in growing lambs: effects of dietary forage levels and source on splanchnic tissue mass in growing lambs.forty-two crossbred lambs (33.4 kg initial body weight; twenty-four wethers and eighteen ewes) were used in a 42 d experiment with a 2 x 3 factorial treatment arrangement to determine effects of forage level and source on splanchnic tissue mass. diets were 250 and 750 g/kg of chopped lucerne (medicago sativa) (a), ryegrass (lolium multiflorum)-wheat (triticum aestivum) (rw) or bermudagrass (cynodon dactylon) (b) hay, with the remainder being maize-based concentrate. five lambs per treatment were ...19948142327
multicentre evaluation of capture assay radim liquid allergen for measurement of specific ige antibodies.a multicentre trial of capture assay radim liquid allergen was performed to define the sensitivity, specificity and clinical reliability of the system in diagnostic allergology. the results of the evaluation were compared with clinical data and in vivo testing. good agreement was obtained for dermatophagoides pteronyssinus (d1), cat's epithelium (e1), betula verrucosa (t3) and olea europea (t9), artemisia vulgaris (w6) and parietaria officinalis (w19). some spreading of data was observed for art ...19958608200
forage characteristics, steer performance, and water quality from bermudagrass pastures fertilized with two levels of nitrogen from swine lagoon effluent.four .8-ha pastures of bermudagrass (cynodon dactylon [l.] pers.) were fertilized with either 456 or 873 kg/ha of nitrogen (n) from swine lagoon effluent (two replicates per treatment) and grazed by steers over two summers. within each pasture, steers received forage only, an energy source (corn), a mixture of corn and soybean meal, or a mixture of corn and blood meal via electronic calan feeders. all supplements were offered at a level of 1.36 kg/d, and the soybean meal and blood meal supplemen ...19968690683
application of nitrogen from swine lagoon effluent to bermudagrass pastures: seasonal changes in forage nitrogenous constituents and effects of energy and escape protein supplementation on beef cattle performance.a 2-yr study was conducted to evaluate 1) the effects of nitrogen level from swine lagoon effluent on forage composition and animal performance and 2) the effects of supplemental escape protein (ep) on performance by steers grazing pastures fertilized with swine lagoon effluent. bermudagrass (cynodon dactylon) pastures were fertilized via sprinkler irrigation with either 448 or 896 kg/ha of n from swine lagoon effluent (two pasture replications/treatment). within each pasture, four supplement tr ...19968726746
cloning and expression in yeast pichia pastoris of a biologically active form of cyn d 1, the major allergen of bermuda grass pollen.pollen of grasses, such as bermuda grass (cynodon dactylon), represent a major cause of type i allergy.19968757211
the carbohydrate moiety of the bermuda grass antigen bg60. new oligosaccharides of plant origin.bg60 is an important allergen of bermuda grass (cynodon dactylon) pollen, which causes allergic responses in human. it was suggested that its carbohydrate moiety may be relevant to allergic reaction (su, s. n., lau, g. x., shu, p., yang, s. y., huang, s. w., and lee, y. c. (1996) j. allergy clin. immunol., in press). therefore, the structure of the carbohydrate moiety in bg60 was investigated. the n-linked oligosaccharides were released from the glycopeptides of bg60 by digesting with a glycoami ...19968900140
net flux of nutrients across splanchnic tissues in wethers consuming grasses of different sources and physical forms ad libitum.crossbred sheep (n 16, 8.5 months of age and 33 (se 0.9) kg) were used in a 21 d experiment (2 x 2 factorial) to determine effects on net flux of nutrients across the portal-drained viscera (pdv) and liver of ad libitum consumption of bermudagrass (cynodon dactylon; b) v. ryegrass (lolium multiflorum)-wheat (triticum aestivum; rw) hay, coarsely chopped (cc) or finely ground and pelleted (gp). crude protein concentrations were 86, 81, 113 and 119 g/kg and neutral-detergent fibre concentrations we ...19979175996
recombinant dna technology in allergology: cloning and expression of plant profilins.profilin, an ubiquitous protein involved in eukaryotic cytoskeleton regulation, has been previously described as allergen in grasses, weeds and trees and in many fruits and vegetables, and it is in part responsible for cross-reactivities pollen and food allergic patients. completed cdna clones coding for phleum pratense, olea europaea, cynodon dactylon, parietaria judaica and helianthus annuus pollen profilins were isolated and sequenced. the deduced amino acid sequences share high identity with ...19979208049
production and characterization of profilin monoclonal antibodies.profilins have been identified as a pan-allergen of different plant pollens and foods. in this paper, we describe the generation of monoclonal antibodies (mabs) by immunizing balb/c mice with helianthus annuus purified profilin in order to characterize this important and common allergen. a panel of forty mabs has been obtained, and twenty of them were used to map antigenic determinants in this molecule. at least two different antigenic determinants were recognized in h. annuus profilin by immuno ...19979208051
effects of supplemental energy source and amount on forage intake and performance by steers and intake and diet digestibility by sheep.two levels of concentrate supplements containing different types of carbohydrates (corn-soybean meal, csbm; wheat middlings, wm; and soybean hulls, sbh) were evaluated for effects on forage intake and performance in growing steers and total diet digestibility in sheep. in exp. 1, 63 crossbred yearling cattle (298 and 377 kg initial bw for yr 1 and 2, respectively) were given ad libitum access to chopped bermudagrass (cynodon dactylon [l.]) hay with no supplementation (control) or with 25 or 50% ...19979222850
[hypersensitivity to common allergens in the central region of coahuila].a retrospective review of 247 clinical files of patient that have responded to the office allergy and immunology since 1990 to 1993 in monclova, coah, for allergy illness. this study was to determine the frequency of aeroalergens and foods sensitization in monclova, coahuila, mediated allergy test skin (intradermo reactions, and prick) and mast immuno systems (multiple allergy system test). the patients were highly sensitive to house dust (13.5%) others aeroalergen pollen grains, were: rusian th ...19939296819
identification of a ca2+ binding protein as a new bermuda grass pollen allergen cyn d 7: ige cross-reactivity with oilseed rape pollen allergen bra r 1.cdna clones encoding two isoforms of an allergen from pollen of bermuda grass (cynodon dactylon) have been isolated using ige from allergic patients. homologous transcripts are present in pollen of 15 other grasses tested. this allergen, tentatively designated as cyn d 7, contains two calcium binding domains and shows significant sequence similarity with other ca2+ binding pollen allergens, namely bet v 4 from birch and bra r 1 from oilseed rape. approximately 10% of allergic sera tested showed ...19979363908
forage substitution in a grain-based diet affects ph and glycogen content of semimembranosus and semitendinosus rabbit muscles.the effects of nutritional level on glycogen content and ph of semimembranosus (sm) and semitendinosus (st) rabbit muscles were investigated. rabbits weaned at 30 d of age were fed one of three diets in which grain had been replaced with 0, 15, and 45% coast cross bermudagrass (cynodon dactylon pers.). muscles were sampled at .5, 3.5, 6.5, and 24 h after slaughter. results showed that these isoenergetic and isoproteic diets did not affect the total number of days required for rabbits to attain 2 ...19979374305
cloning and high level expression of cynodon dactylon (bermuda grass) pollen profilin (cyn d 12) in escherichia coli: purification and characterization of the allergen.profilin, an actin-binding protein, was previously described as a panallergen which is involved in about 20% of the crossreactivity found among pollen and food allergic patients. this allergen is usually under-represented in natural extracts used for allergy diagnosis.19979420135
[cutaneous hypersensitivity in patients with bronchial asthma in la comarca lagunera].a cross-sectional study was done to determine the frequency of cutaneous sensibility to aeroallergens and variations related to residence area. in a period of time from june 1994 to june 1995, we analyzed 101 patients residents in the comarca lagunera diagnosed as having allergic bronchial asthma. the patients were highly sensitive to pollen grains: cynodon dactylon (70%), chenopodium (69%), rusian thistle (63%), rye grass (61%), zea maiz and prosopis (57%). the sensitivity to molds was less: ca ...19979477662
ige antibodies to recombinant pollen allergens (phl p 1, phl p 2, phl p 5, and bet v 2) account for a high percentage of grass pollen-specific ige.pollen from different grass species are some of the most potent elicitors of type i allergy worldwide. the characterization of antigenic structures and ige epitopes common to different grass species is relevant to define reagents for diagnosis and specific therapy of grass pollen allergy.19989500760
prevalence of atopy in students from málaga, spain.epidemiologic studies are necessary to determine the prevalence of allergic diseases. this varies widely depending on allergen preparations and patients studied.19989532972
how far can we simplify in vitro diagnostics for grass pollen allergy?: a study with 17 whole pollen extracts and purified natural and recombinant major allergens.current diagnostics for grass pollen allergy are composed of mixtures of pollen of different grass species. their complex composition hampers accurate standardization.19989723659
[diagnostic use of enzymatic rast skin tests and determination of eosinophils in nasal mucosa in allergic rhinitis].allergic rhinitis is the most frequent disease mediated by immunoglobulin e (ige). nasal challenge is the gold standard for the diagnosis of allergic rhinitis. skin tests (st) are the most used diagnostic method to detect the presence of specific ige bind to skin mast cells. the exposition of the nasal mucous membrane to the allergen is followed by an increase of the local eosinophils; the count of eosinophils in nasal mucous (enm) is a diagnostic test for allergic rhinitis. enzymatic rast or en ...19989951327
voluntary intake, digestibility, and subsequent selection of matua bromegrass, coastal bermudagrass, and alfalfa hays by yearling horses.matua bromegrass (bromus willdenowii kunth. cv. grasslands matua) was introduced in 1973, but little information exists concerning its potential as a hay for horses. thus, voluntary intake and apparent digestibility of om, cp, and fiber components of matua by 18 quarter horse yearlings (mean initial bw 354 kg; se 5.8) were compared with alfalfa (medicago sativa l.) and coastal bermudagrass (cynodon dactylon l.) as hays in a randomized block design. a 15-d adjustment period was followed by a 5-d ...199910521039
influence of chromium tripicolinate on growth and glucose metabolism in yearling horses.thoroughbred and quarter horse yearlings (n = 24; 335+/-7 d of age) were used in a 112-d feeding trial to determine whether chromium (cr) supplementation would alter growth, development, and energy metabolism of growing horses on high-concentrate diets. the horses were assigned at random within breed and gender subgroups to one of four treatment groups: a) basal concentrate; b) basal plus 175 microg of cr/kg concentrate; c) basal plus 350 microg of cr/kg concentrate; and d) basal plus 700 microg ...199910568473
a clinical study of airborne allergens in the united arab emirates.in the past 25 years the united arab emirates (uae) have experienced a socioeconomic boom. the once nomadic bedouin population of al ain, in the emirate of abu dhabi, now lives in modern air-conditioned accommodation, and huge desalination plants have allowed afforestation and farming.199910572581
substrate preference profiles of proteases released by allergenic pollens.pollens are important triggers for allergic asthma and seasonal rhinitis. we have recently reported that proteases released by major allergenic pollens can injure airway epithelial cells in vitro. disruption of epithelial integrity by proteases released following deposition of pollens on mucosal surfaces could promote sensitization and induce inflammation.200010718856
short communication: effect of tannic acid on composition and ruminal degradability of bermudagrass and alfalfa silages.we measured the effects of the addition of tannic acid on chemical composition and crude protein (cp) ruminal degradability of bermudagrass, cv. coast cross (cynodon dactylon l.) and alfalfa (medicago sativa l.) silages with nylon bags incubated in the rumen of two fistulated lactating cows. silage ph was greater for alfalfa than for bermudagrass. the addition of tannic acid had no effect on silage ph. ammonia n was greater in alfalfa than in bermudagrass silage. treated and control silages had ...200011003232
modified aflp technique for rapid genetic characterization in plants.the standard amplified fragment-length polymorphism (aflp) technique was modified to develop a convenient and reliable technique for rapid genetic characterization of plants. modifications included (i) using one restriction enzyme, one adapter molecule and primer, (ii) incorporating formamide to generate more intense and uniform bands and (iii) using agarose gel electrophoresis. sea oats (uniola paniculata l.), pickerel-weed (pontederia cordata l.), bermudagrass (cynodon dactylon l.) and penstem ...200011056817
effect of maturity on degradation kinetics of sod-seeded cereal grain forage grown in northern arkansas.wheat (triticum aestivum l.), oat (avena sativa l.), and rye (secale cereale l.) were overseeded into a dormant bermudagrass (cynodon dactylon (l.) pers.) sod and harvested at 3-wk intervals throughout march, april, may, and early june. plant growth stage was documented for each forage on each harvest date, and harvested forages were evaluated for forage quality characteristics. degradation kinetics of dm and ndf for these forages were evaluated by the in situ method. fractional degradation rate ...200011104269
feasibility of using lignin isolated from forages by solubilization in acetyl bromide as a standard for lignin analyses.lignin concentration can be measured in plants by the acetyl bromide-soluble lignin spectrophotometric method; however, as with any spectrophotometric method, a reliable standard is needed. in the present experiments, lignin was extracted from each of the forages under study with the acetyl bromide reagent. the lignin isolated with acetyl bromide (liab) was then used as the reference standard in the acetyl bromide-soluble lignin (absl) analysis, which was compared with the acid detergent lignin ...200011132828
marker concentration patterns of labelled leaf and stem particles in the rumen of cattle grazing bermuda grass (cynodon dactylon) analysed by reference to a raft model.large (>1600 microm), ingestively masticated particles of bermuda grass (cynodon dactylon l. pers.) leaf and stem labelled with 169yb and 144ce respectively were inserted into the rumen digesta raft of heifers grazing bermuda grass. the concentration of markers in digesta sampled from the raft and ventral rumen were monitored at regular intervals over approximately 144 h. the data from the two sampling sites were simultaneously fitted to two pool (raft and ventral rumen-reticulum) models with ei ...200111348570
isolation and partial characterization of a 46-kd allergen of bermuda grass pollen.cyn d bd46k, a 46-kd component of bermuda grass (cynodon dactylon) pollen, had been identified as an allergenic constituent. in the present study two-dimensional (2d) gel electrophoresis illustrated the presence of five acidic isoforms in cyn d bd46k, and this molecule was purified by monoclonal antibody (mab) affinity chromatography for further characterization. using a digoxigenin-labeled lectin-binding assay, the elucidating protein was disclosed to be a glycoprotein with terminal mannose. th ...200111455197
phosphorus and ammonium concentrations in surface runoff from grasslands fertilized with broiler litter.application of broiler (gallus gallus domesticus) litter to grasslands can increase ammonium (nh4-n) and dissolved reactive phosphorus (drp) concentrations in surface runoff, but it is not known for how long after a broiler litter application that these concentrations remain elevated. this long-term study was conducted to measure nh4-n and drp in surface runoff from grasslands fertilized with broiler litter. six 0.75-ha, fescue (festuca arundinacea schreb.-)bermudagrass [cynodon dactylon (l.) pe ...200111577887
phosphorus losses from grasslands fertilized with broiler litter: epic simulations.broiler litter, a mixture of poultry excreta and bedding material, is commonly used to fertilize grasslands in the southeastern usa. previous work has shown that under certain situations, application of broiler (gallus gallus domesticus) litter to grasslands may lead to elevated levels of phosphorus (p) in surface runoff. the epic simulation model may be a useful tool to identify those situations. this work was conducted to evaluate epic's ability to simulate event and annual runoff volume and l ...200111577888
impact of heating-degree-day accumulation during bermudagrass hay storage on nutrient utilization by lambs.spontaneous heating in stored alfalfa (medicago sativa) has reduced forage quality and dm and n digestibility through microbial respiration and maillard polymer formation. the impact of heating-degree-day (hdd) accumulation in stored bermudagrass (cynodon dactylon [l.] pers.) was evaluated for its effect on nutrient utilization by lambs. twenty rambouillet wether lambs (53.1 +/- 0.74 kg) were used in a completely randomized total collection experiment. the hays selected for this study had previo ...200111721850
effects of calendar date and summer management on the in situ dry matter and fiber degradation of stockpiled forage from bermudagrass pastures.limited information is available that describes the disappearance kinetics of bermudagrass (cynodon dactylon l. pers.) during fall and early winter. five ruminally cannulated, crossbred steers (387 +/- 18.3 kg) were used to determine the effects of calendar date and previous summer management on the in situ degradation kinetics of dm and ndf for forage clipped from stockpiled 'greenfield' bermudagrass pastures. forage was stockpiled at two sites following summer hay or pasture management, and sa ...200111811472
molecular phylogeny of hybridizing species from the genus spartina schreb. (poaceae).interspecific hybridization events have been reported in the genus spartina schreb. (poaceae), involving the east american species spartina alterniflora, and including either introgression (e.g., with the western american spartina foliosa) or allopolyploid speciation (e.g., with the euro-african spartina maritima). molecular phylogenetic analysis of the genus has been undertaken in order to understand phylogenetic relationships and genetic divergence among these hybridizing species. twelve spart ...200211820850
molecular and serological relationships of spartina mottle virus (spmv) strains from spartina spec. and from cynodon dactylon to other members of the potyviridae.spartina mottle virus (spmv) was first reported 1980 and classified by physical and biological properties as a tentative member of the genus rymovirus in the potyviridae. this genus was recently separated into two genera: rymovirus and tritimovirus. now the sequence of the 3'-terminal part of the genome of spmv was determined. additionally a virus isolate originating from cynodon dactylon in italy was cloned and sequenced. this assisi-isolate shared 87.5% amino acid sequence identity with spmv. ...200211890529
oligoclonal analysis of the atopic t cell response to the group 1 allergen of cynodon dactylon (bermuda grass) pollen: pre- and post-allergen-specific immunotherapy.bermuda grass pollen (bgp) is an increasingly important seasonal aeroallergen in australia and other subtropical and temperate regions. bgp shares minimal allergenic cross-reactivity with pollens of rye grass or other pooideae grasses often used for desensitization regimens in grass pollen allergy. current allergen immunotherapy is seldom used in asthmatic patients due to ige-mediated side effects. since clinically effective immunotherapy is linked with altered allergen-specific t cell response, ...200211979049
southeastern pasture-based dairy systems: housing, posilac, and supplemental silage effects on cow performance.this experiment tested performance and physiological responses to evaporative cooling, bovine somatotropin (bst), and supplemental silage of lactating cows grazing bermudagrass (tifton 85; cynodon dactylon x c. nlemfuensis cv.) pastures. multiparous (n = 32) cows (196 d in milk) were assigned one of five treatments arranged in two replicates. treatments were 1) cows maintained continuously on pasture with access to shade, 2) treatment 1 + bst, 3) night housing on pasture, then free-stall housing ...200212018432
winter-annual pasture as a supplement for beef cows.in each of two experiments, 120 pregnant beef cows were stratified by body condition score, bw, breed, and age, randomly divided into six groups of 20, and assigned to one of six 5.1-ha bermudagrass (cynodon dactylon [l.] pers.) pastures (two replicates/ treatment) in early january to evaluate the use of winter-annual pasture as a supplement. all cows in exp. 1 and 2 had ad libitum access to bermudagrass/dallisgrass (paspalum dilatatum poir.) hay plus three treatments: 1) a concentrate-based sup ...200212019601
cross-reactivities between date palm (phoenix dactylifera l.) polypeptides and foods implicated in the oral allergy syndrome.date fruit and pollen antigens share a number of cross-reactive epitopes. date pollen has been shown to cross-react with antigens from artemisia, cultivated rye (secale cereale), timothy grass (phleum pratense), sydney golden wattle (acacia longifolia) and bermuda grass (cynodon dactylon) pollen. the present study was carried out to examine any cross-reactivities between date palm polypeptides and antigens of some common foods and vegetables that have been implicated in the oral allergy syndrome ...200212028116
bioaccumulation of as, cd, cu, fe and pb in wild grasses affected by the aznalcóllar mine spill (sw spain).the collapse of the tailing dam in the aznalcóllar pyrite mine (sw spain) occurred in april 1998 and affected approximately 4300 ha along the agrio and guadiamar valleys. an urgent soil cleaning up and remediation programme was started just after the accident. eighteen months later, mineral nutrients and trace elements concentration in soil and two wild grasses--cynodon dactylon and sorghum halepense--have been studied. three types of conditions are distinguished: (a) unaffected soils (control); ...200212083702
heavy metals and arsenic uptake by wild vegetation in the guadiamar river area after the toxic spill of the aznalcóllar mine.on 25 april 1998, approximately 4.5 hm(3) of pyritic sludge, containing 5000 mg of as kg(-1) among other pollutants, was spilled into the agrio and guadiamar rivers and the surrounding agricultural areas (aznalcóllar, seville, southern spain). many trace metals such as pb, cu, zn, cd, tl, sb and as reached the doñana national park, the largest wetland area in europe, affecting soils, different plant and animal species. in order to recuperate the affected lands by employing plants capable of accu ...200212126811
effect of grazing on plant attributes and hydrological properties in the sloping lands of the east african highlands.extending livestock grazing to the steep slopes has led to unstable grazing systems in the east african highlands, and new solutions and approaches are needed to ameliorate the current situation. this work was aimed at studying the effect of livestock grazing on plant attributes and hydrological properties. the study was conducted from 1996 to 2000 at the international livestock research institute at debre ziet research station. two sites were selected: one at 0-4% slope, and the other at 4-8% s ...200212148074
export of manure phosphorus and nitrogen in turfgrass sod.regulatory mandates have increased demand for best management practices (bmps) that will reduce nutrient loading on watersheds impaired by excess manure p and n. export of manure p and n in turfgrass sod harvests is one bmp under consideration. this study quantified amounts and percentages of p and n removed in a sod harvest for different rates of manure and inorganic p and n. six treatments comprised an unfertilized control, two manure rates with and without supplemental inorganic n, and inorga ...200212371193
[influence of saltwater irrigation on the yield and quality of cynodon dactylon under desert conditions].responses of six varieties (suwannee, coast cross, tifton44, tifton68, tifton78 and tifton85) of cynodon dactylon to irrigation-water salinity were investigated in field by means of a double line source experimental design. the digestibility of the grass by goat was analyzed using the rumen gastric justice digestion method. the results showed that the six varieties grew well, and had a high yield of fresh grass when eletro-conductivity (eci) < 10 ds.m-1. particularly when eci = 4.4 ds.m-1, the f ...200212418261
long-term biosolids application effects on metal concentrations in soil and bermudagrass forage.the long-term application of biosolids that periodically contained elevated metal concentrations has raised questions about potential effects on animal health. to address these concerns, we determined metal concentrations (as, cd, cu, pb, hg, mo, ni, se, and zn) in both soil and bermudagrass [cynodon dactylon (l.) pers.] forage from 10 fields in the following categories of biosolids application: six or more years (>6yr), less than six years (<6yr), and no applications (ns). soil metal concentrat ...200312549553
methane emissions of beef cattle on forages: efficiency of grazing management systems.fermentation in the rumen of cattle produces methane (ch4). methane may play a role in global warming scenarios. the linking of grazing management strategies to more efficient beef production while reducing the ch4 emitted by beef cattle is important. the sulfur hexafluoride (sf6) tracer technique was used to determine the effects of best management practices (bmp) grazing compared with continuous grazing on ch4 production in several louisiana forages during 1996-1998. cows and heifers (bos taur ...200312549566
screening of antioxidant activity of three indian medicinal plants, traditionally used for the management of neurodegenerative diseases.a number of indian medicinal plants have been used for thousands of years in the traditional system of medicine (ayurveda). amongst these are plants used for the management of neurodegenerative diseases such as parkinson's, alzheimer's, loss of memory, degeneration of nerves and other neuronal disorders by the ayurvedic practitioners. though the etiology of neurodegenerative diseases remains enigmatic, there is evidence, which indicates that defective energy metabolism, excitotoxicity and oxidat ...200312648805
swine effluent irrigation rate and timing effects on bermudagrass growth, nitrogen and phosphorus utilization, and residual soil nitrogen.maximizing utilization of effluent nutrients by forage grasses requires a better understanding of irrigation rate and timing effects. this study was conducted in 1998 and 1999 on a vaiden silty clay (very-fine, smectitic, thermic aquic dystrudert) soil to determine the effects of swine lagoon effluent irrigation rate and timing on bermudagrass [cynodon dactylon (l.) pers.] growth, nitrogen (n) and phosphorus (p) recovery, and postseason soil profile no3(-)-n. treatments consisted of swine efflue ...200312708693
effects of supplementary selenium source on the performance and blood measurements in beef cows and their calves.on december 2, 1999, 120 pregnant cows were weighed, their body condition scored, and then sorted into six groups of 20 stratified by bcs, bw, breed, and age. groups were assigned randomly to six, 5.1-ha dormant common bermudagrass (cynodon dactylon [l.] pers.) pastures for 2 yr to determine the effects of supplemental se and its source on performance and blood measurements. during the winter, each group of cows had ad libitum access to bermudagrass/dallisgrass (paspalum dilatatum poir.) hay plu ...200312723072
bermudagrass management in the southern piedmont u.s. iv. soil surface nitrogen pools.the fate of nitrogen (n) applied in forage-based agricultural systems is important for understanding the long-term production and environmental impacts of a particular management strategy. we evaluated the factorial combination of three types of n fertilization (inorganic, crimson clover [trifolium incarnatum l.] cover crop plus inorganic, and chicken [gallus gallus] broiler litter pressure and four types of harvest strategy (unharvested forage, low and high cattle [bos taurus] grazing pressure, ...200112806068
[vegetation state and soil enzyme activities of copper tailing yard on tongguan mountain].from the open investigation and laboratory analysis, this paper studied the vegetation state and soil enzyme activities of copper tailing yard of tongguan mountain. the results showed that there were 34 species of natural colonized plants on copper tailing yard, subordinated to 16 families and 33 genera, and regard herbs as principle, and many for 1-2 years old. the main families were compositae (10 species), gramineae (9 species) and legumineceae (2 species). hippochaete ramosissimum, which bel ...200312924135
bermudagrass management in the southern piedmont usa: vi. soil-profile inorganic nitrogen.fate of applied n in forage-based agricultural systems is important to long-term production and environmental impacts. we evaluated the factorial combination of n fertilization targeted to supply 20 g n m 2 yr(-1) and harvest strategies on soil-profile inorganic n during the first 5 yr of 'coastal' bermudagrass [cynodon dactylon (l.) pers.] management. harvest strategy had much larger effects than fertilization strategy, most notably that soil-profile inorganic n was lower when hayed than under ...200312931887
mass spectrometric analysis of electrophoretically separated allergens and proteases in grass pollen diffusates.pollens are important triggers for allergic asthma and seasonal rhinitis, and proteases released by major allergenic pollens can injure airway epithelial cells in vitro. disruption of mucosal epithelial integrity by proteases released by inhaled pollens could promote allergic sensitisation.200314577842
water-quality effects of incorporating poultry litter into perennial grassland soils.poultry litter provides a rich source of nutrients for perennial forages, but the usual practice of surface-applying litter to pastures can degrade water quality by allowing nutrients to be transported from fields in surface runoff, while much of the nh4-n volatilizes. incorporating litter into the soil can minimize such problems in tilled systems, but has not been used for perennial forage systems. in this study, we minimized disturbance of the crop, thatch, and soil structure by using a knifin ...200314674565
a potent feed preservative candidate produced by calcarisporium sp., an endophyte residing in stargrass (cynodon dactylon).the cultures of an endophytic fungus calcarisporium sp. were screened for inhibitors on the growth of feed-associated moulds and on the aflatoxin biosynthesis to find a safe and effective feed preservative.200414723696
runoff losses of phosphorus and nitrogen imported in sod or composted manure for turf establishment.nutrient loading on impaired watersheds can be reduced through export of sod grown with manure and export of composted manure for turf production on other watersheds. effects of the sod and manure exports on receiving watersheds were evaluated through monitoring of total dissolved phosphorus (tdp) and n concentrations and losses in runoff from establishing turf. three replications of seven treatments were established on an 8.5% slope of a booneville soil (loamy-skeletal, mixed, superactive pachi ...200414964391
turfgrass, crop, and weed hosts of blissus occiduus (hemiptera: lygaeidae).blissus occiduus barber is an important pest of buffalograss, buchloë dactyloides (nuttall) engelmann, turf. no-choice studies documented the susceptibility of selected turfgrasses, crops, and weeds to b. occiduus feeding. highly to moderately susceptible grasses included buffalograss; yellow setaria glauca (l.) and green foxtail setaria viridis (l.); kentucky bluegrass, poa pratensis l.; perennial ryegrass, lolium perenne l.; brome, bromus spp. leyss.; zoysiagrass, zoysia japonica steudel; berm ...200414998128
bermudagrass sod growth and metal uptake in coal combustion by-product-amended media.coal combustion by-products (ccb) include fly ash and bottom ash and are generated nationally at rates of 10(8) mg yr(-1). land applications of ccb have improved physicochemical properties of soil, yet inherent bulkiness and trace metal content of ccb often limit their use. likewise, utilization of biosolids and manure as fertilizer can be problematic due to unfavorable nutrient ratios. a 2-yr field study evaluated environmental and technical parameters associated with ccb-organic waste utilizat ...200415074828
bermudagrass management in the southern piedmont, usa: ix. trace elements in soil with broiler litter application.an understanding of the long-term cycling of trace elements in soil with broiler litter fertilization under various forage utilization strategies is needed to develop sustainable agricultural production systems. we evaluated differences in cu, mn, zn, and six other trace elements in response to 5 yr of bermudagrass [cynodon dactylon (l.) pers.] management varying in fertilization and harvest strategies on a typic kanhapludult in georgia. chicken (gallus gallus) broiler litter was a significant s ...200415074832
molecular characterization of polygalacturonases as grass pollen-specific marker allergens: expulsion from pollen via submicronic respirable particles.grass pollen belong to the most important allergen sources involved in the elicitation of allergic asthma. we have isolated cdnas coding for bermuda grass (cynodon dactylon) and timothy grass (phleum pratense) pollen allergens, belonging to a family of pectin-degrading enzymes (i.e., polygalacturonases). the corresponding allergens, termed cyn d 13 and phl p 13, represent glycoproteins of approximately 42 kda and isoelectric points of 7.5. rphl p 13 was expressed in escherichia coli and purified ...200415128842
anti-helicobacter pylori metabolites from rhizoctonia sp. cy064, an endophytic fungus in cynodon dactylon.a new benzophenone, named rhizoctonic acid (1), together with three known compounds monomethylsulochrin (2), ergosterol (3) and 3beta,5alpha,6beta-trihydroxyergosta-7,22-diene (4) were isolated through bioassay-guided fractionations from the culture of rhizoctonia sp. (cy064), an endophytic fungus in the leaf of cynodon dactylon. the structure of the new acid 1 was elucidated to be 5-hydroxy-2-(2-hydroxy-6-methoxy-4-methylbenzoyl)-3-methoxybenzoic acid by a combination of spectral analyses. furt ...200415261382
'candidatus phytoplasma cynodontis', the phytoplasma associated with bermuda grass white leaf disease.bermuda grass white leaf (bgwl) is a destructive, phytoplasmal disease of bermuda grass (cynodon dactylon). the causal pathogen, the bgwl agent, differs from other phytoplasmas that cluster in the same major branch of the phytoplasma phylogenetic clade in <2.5% of 16s rdna nucleotide positions, the threshold for assigning species rank to phytoplasmas under the provisional status 'candidatus'. thus, the objective of this work was to examine homogeneity of bgwl isolates and to determine whether th ...200415280272
the evaluation of nitric oxide scavenging activity of certain indian medicinal plants in vitro: a preliminary study.the plant extracts of 17 commonly used indian medicinal plants were examined for their possible regulatory effect on nitric oxide (no) levels using sodium nitroprusside as an no donor in vitro. most of the plant extracts tested demonstrated direct scavenging of no and exhibited significant activity. the potency of scavenging activity was in the following order: alstonia scholaris > cynodon dactylon > morinda citrifolia > tylophora indica > tectona grandis > aegle marmelos (leaf) > momordica char ...200415383230
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