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[cytochemical characteristics of the labrocyte-like cells in the capsule of the plerocercoids of triaenophorus nodulosus (pallas, 1781) and diphyllobothrium dendriticum (nitzsch, 1824) cestodes].out of six fish species examined (esox lucius, paracottus kessleri, perca fluviatilis, leuciscus leuciscus baicalensis, coregonus autumnalis migratorius, thymallus arcticus baicalensis) labrocyte-like cells were detected in esox lucius, leuciscus leuciscus baicalensis, coregonus autumnalis migratotius. the cells contain large metachromatic granules. histochemical methods revealed in them sulfated and carboxylic acid mucopolysacharides. the amount of labrocyte-like cells in the capsules around pl ...1977143258
[changes in the argyrophil stroma of the liver of fishes infested with the pleurocercoids, triaenophorus nodulosus and diphyllobothrium dendriticum (cestoidea, pseudophyllidae)].the argentaffine stroma of the liver of some fishes (perca fluviatilis, exos lucius, paracottus kessleri, coregonus autumnalis migratorius, thymallus arcticus) is described. the nature and depth of pathological changes in the argentaffine stroma of the liver at its infection with pleurocercoids of pseudophyllids depend on the infection intensity, host's specificity and on the biocoenotic relationships of hydrobionts in a waterbody.1977896280
sequential development of the immune response in rainbow trout [oncorhynchus mykiss (walbaum, 1792)] to experimental plerocercoid infections of diphyllobothrium dendriticum (nitzsch, 1824).development of the inflammatory response of rainbow trout to experimental infections with diphyllobothrium dendriticum plerocercoids is described using light and electron microscopy. the cellular response to plerocercoids occurred within 2 weeks post-infection (p.i.). this was followed by an increase in leucocyte numbers during weeks 3-6 p.i., with full encapsulation of plerocercoids by week 6 p.i. neutrophils were the first leucocytes to engage the developing plerocercoid, followed by large inf ...19921614733
[the survival of diphyllobothrium dendriticum pleurocercoids during the processing of fish and sanitary helminthologic evaluation of the product].diphyllobothrium dendriticum plerocercoids' survival in salt water solutions of various concentrations and at various temperature was assessed. conditions of salt treatment and freezing of the baikal salmon assuring total release of fish from d. dendriticum plerocercoids are determined. the authors suggested differentiation in sanitary helminthological assessment of fish invasion rate by d. latum and d. dendriticum plerocercoids.19892811750
[composition of definite hosts of diphyllobothrium dendriticum (nitzsch, 1824) and distribution of its imago hemipopulation in the baikal water basin]. 19883231172
[the agglutination test of coracidia of pseudophyllidean cestodes and its use in cestodological research].ability of coracidia to agglutination in homologous antiserum was established. the method of reaction of a direct agglutination of coracidia (rdac) in the culture of alive larvae was described. when comparing the rdac results in homologous and heterologous variants a dependence of the agglutination pattern on the degree of antiserum homology was established. on the example of rdac of the cestodes diphyllobothrium dendriticum and d. ditremum in polyvalent and species-specific antisera to d. dendr ...19854047715
coracidia agglutination reaction of pseudophyllid cestodes and its application in cestode studies.the ability of cestode coracidia to agglutinate in homologous antiserum was established. the method of direct agglutination of coracidia (rdac) in a culture of living larvae is described. the comparison of rdac results in homologous and heterologous variants shows a dependence of the agglutination pattern on the degree of antiserum homology. by the example of rdac of the cestodes diphyllobothrium dentriticum and d. ditremum in polyvalent antisera and in antisera species-specific to d. dendriticu ...19854025910
histochemical studies of the cestode diphyllobothrium dendriticum nitzsch, 1824. 19684303248
some effects of population density in infections of diphyllobothrium dendriticum (nitzsch) in golden hamster (mesocricetus auratus waterhouse) and common gull (larus canus l.). 19744417091
differentiation of the tegument and associated structures in diphyllobothrium dendriticum nitsch (1824) (cestoda: pseudophyllidea). an electron microscopical study. 19734732029
the effect of praziquantel, an antihelminthicum, on the peptidergic nervous system of the tapeworm diphyllobothrium dendriticum.the effect of the antihelminthic substance praziquantel on the peptidergic nervous system in the adult gull-tapeworm diphyllobothrium dendriticum was tested. as well as increasing muscle contraction the drug provoked a fall in the amount and size of the peptidergic nerve terminals in the subtegumental region. the number of small clear vesicles in the terminals also fell.19853999818
duration of the cell cycle of germinative cells in plerocercoids of diphyllobothrium dendriticum. 19675613881
immunocytochemical demonstration of neuropeptides and serotonin in the tapeworm diphyllobothrium dendriticum.the present immunocytochemical study concerns the distribution of four neuropeptides, fmrf-amide, vasotocin, leu-enkephalin and neurotensin, and of the bioamine serotonin in the plerocercoid larva of diphyllobothrium dendriticum. anti-fmrf-amide and vasotocin-reactivity occurs in perikarya and nerve fibres in the cns and pns of this worm. the peptide-containing fibres surround and seem to innervate the musculature and to terminate beneath the basal lamina of the tegument at the inner surface of ...19853995550
[infectivity of fishes in the lake baikal basin with plerocercoids of diphyllobothrium dendriticum]. 19883193925
immunocytochemical evidence for the presence of "mammalian" neurohormonal peptides in neurones of the tapeworm diphyllobothrium dendriticum.in the nervous system of the obligatory endoparasite diphyllobothrium dendriticum immunoreactivity (ir) to growth hormone-releasing factor (grf), peptide histidine isoleucine (phi), bovine pancreatic polypeptide (bpp), gastrin, gastrin-releasing peptide (grp), oxytocin, fmrfamide (fmrf) and serotonin (5ht) was demonstrated by immunocytochemical methods. a very strong grf-ir was observed in the cns and pns of larvae and of the constantly growing adult worms. grf-ir axon terminals occur beneath th ...19863080246
[epidemiology of diphyllobothriasis in the valdivia river basin, chile].in the valdivia (chile) river basin 1,295 inhabitants of 6 riverside districts were examined between march and october 1987 and showed a 1.2% of prevalence by diphyllobothrium in the districts of riñihue and las huellas. prevalence of 5.3% and 9.8% respectively were registered in dogs of the districts of riñihue and malihue. no cat or pig infection was observed in the different districts. the parasites recovered after the treatment were identified as diphyllobothrium latum. human infection by d. ...19892814310
dynamics of egg production of the cestode diphyllobothrium dendriticum (nitzsch, 1824) (cestoda: pseudophyllidea) and the concept of fecundity in helminths.the dynamics of egg production of the tapeworm d. dendriticum has been estimated experimentally in nestlings of the herring gull larus argentatus per day and per reproductive period. numbers of eggs in strobilae have been estimated for the maturation period. mean egg production, of one tapeworm per day (10.42 +/- 2.72 mln) is two orders of magnitude as high as the maximal number of eggs in a mature strobila (0.206 +/- 0.007). it is proposed to estimate the coefficient of reproduction intensity a ...19892767550
[cestode egg production dynamics and fertility in diphyllobothrium dendriticum (cestoda, pseudophyllidea)].dynamics of egg production of the tapeworm diphyllobothrium dendriticum has been estimated experimentally in nestlings of the herring gull larus argentatus per day and per reproductive period. the number of eggs in strobiles has been estimated for the maturation period. mean egg production of the tapeworm per day (10.43 +/- 2.72 mln) is two orders of magnitude as high as the maximum number of eggs in a mature strobile (0.206 +/- 0.007). it is proposed to estimate coefficient of reproduction inte ...19892748199
scanning electron microscopic study of four diphyllobothrium species.three-dimensional observation was carried out on plerocercoids and adults of diphyllobothrium dendriticum, d. ditremum, d. latum, and d. vogeli using scanning electron microscopy. the species-specific differences between plerocercoids were recognized in the shapes of the whole body, scolex, and bothrium and the wrinkle pattern on the body surface. the differences between adult worms were also observed in the shapes of the scolex, neck, and genital papillae around the genital pore and the pattern ...19892710776
high-performance liquid chromatographic analysis of monoamines in the cestode diphyllobothrium dendriticum.the presence of biogene monoamines in adult and larval diphyllobothrium dendriticum (cestoda) was investigated by high-performance liquid chromatography with electrochemical detection (hplc-ed). the biogene amines serotonin (5-ht), dopamine (da), noradrenaline (na), and adrenaline (a) as well as many of their precursors and metabolites, comprising a total of 15 different substances, were analyzed. 5-ht, dopa, da, na, and a were detected in the worm, with 5-ht, dopa, and da being the dominating a ...19938295909
development of immunoreactivity to the invertebrate neuropeptide small cardiac peptide b in the tapeworm diphyllobothrium dendriticum.neurons immunoreactive to small cardiac peptide b (scpb) occur in the scolex and neck region of adult diphyllobothrium dendriticum. the localisation of the scpb-ir neurons in the peripheral nervous system is very pronounced; they are closely associated to the bothridial musculature in the scolex. scpb-ir neurons were not observed in plerocercoid larvae but appeared after cultivation in vitro at 37 degrees c for 30 h. functional and developmental aspects of the scpb-ir neurons are discussed.19892657717
[antigenic erythrocyte diagnostic agents of 2 specificities (diphyllobothrium dendriticum and d. ditremum) and their use for detecting fecal antibodies in cestodiases]. 19872452339
immunocytochemical localization of histamine in flatworms.specific antibodies against histamine were used to demonstrate the occurrence and cellular distribution of histamine-like immunoreactivity in three species of flatworms (phylum platyhelminthes). in the parasitic cestode diphyllobothrium dendriticum, histamine-reactivity was found in neurons of the main nerve cords, and in cells lining the central and peripheral excretory ducts. in the free-living microturbellarian microstomum lineare and in the planarian polycelis nigra, histamine-immunoreactivi ...19902372807
the laboratory maintenance of diphyllobothrium dendriticum (nitzsch, 1824).a protocol for the routine laboratory maintenance of the life-cycle of diphyllobothrium dendriticum, using herring gulls (larus argentatus), cyclops abyssorum and rainbow trout oncorhynchus mykiss ( = salmo gairdneri) as the definitive, first and second intermediate hosts respectively, is described.19902235071
the ecology of echinococcus multilocularis (cestoda: taeniidae) on st. lawrence island, alaska. ii. helminth populations in the definitive host.the helminths of 1,579 arctic foxes from st. lawrence island were investigated by standard methods. the foxes, obtained mainly during the winter from fur trappers, harbored 22 species of helminths. four of those were trematodes, viz., maritrema afanassjewi belopol'skaia, 1952, orthosplanchnus pygmaeus iurakhno, 1967, plagiorchis elegans (rudolphi, 1802) and alaria marcianae (larue, 1917), each of which occurred in a single host. two species of cestodes, diphyllobothrium dendriticum (nitzsch, 182 ...19902080830
skin the tapeworms before you stain their nervous system! a new method for whole-mount immunocytochemistry.a new method for making whole-mount immunocytochemical preparations of thick-skinned tapeworms is described. the tough tegument of the worms, which is impermeable to primary and secondary antisera, is removed by placing live worms in distilled water for 5-8 h, during which period the tegument is shed. the worms are thereafter fixed and stained as whole specimens using fluorescence immunocytochemical techniques. both the main nervous structures and the finest neuronal details are revealed by this ...19911924258
localization and identification of catecholamines in the nervous system of diphyllobothrium dendriticum (cestoda).in the central and peripheral nervous system of larval and adult diphyllobothrium dendriticum, catecholamines were detected using the glyoxylic acid-induced fluorescence method. high-pressure liquid chromatography (hplc) analysis revealed the presence of dopa (about 65 ng/g fresh weight) and dopamine (5 ng/g fresh weight).19911924256
leucocyte migration in rainbow trout (oncorhynchus mykiss [walbaum]): optimization of migration conditions and responses to host and pathogen (diphyllobothrium dendriticum [nitzsch]) derived chemoattractants.a rainbow trout leucocyte-derived chemoattractant(s) was prepared and tested as a stimulant of leucocyte migration. it was used to optimize an in vitro leucocyte migration assay using a 48-well micro chemotaxis chamber. this assay has subsequently been used to test the chemoattractant activity of antigen extracts from the tegument of diphyllobothrium dendriticum plerocercoids and conditioned medium obtained after in vitro maintenance of live plerocercoids. leucocytes were found to have an increa ...19911773854
a hidden break in the 28.0s rrna from diphyllobothrium dendriticum.nondenatured and denatured total rna from the tapeworm diphyllobothrium dendriticum (cestoda) was analysed by agarose gel electrophoresis. it was found that the large subunit ribosomal rna (lrrna) is 28.0s and the small subunit ribosomal rna (srrna) is 19.5s. following denaturation the 28.0s rrna was disrupted into a 19.5s subfragment and a 20.7s subfragment due to the presence of a centrally located hidden break. by hybridization of northern blot membranes with oligonucleotide probes specific f ...19921452994
never ending growth and a growth factor. i. immunocytochemical evidence for the presence of basic fibroblast growth factor in a tapeworm.basic fibroblast growth factor immunoreactivity (bfgf-ir) was detected in a specific set of nerve cells in the central nervous system of the gull-tapeworm diphyllobothrium dendriticum (cestoda)--an obligatory endoparasite. the long varicose processes of the bfgf-ir neurons extend along the two main nerve cords, which contain an actively growing population of nerve cells. a neurotrophic function for the bfgf-like material is proposed. the adult tapeworm lives in the intestine of a homeothermic ho ...19921306687
studies on cytodifferentiation in the neck region of diphyllobothrium dendriticum nitzsch, 1824 (cestoda, pseudophyllidea).cytodifferentiation in the neck region of adult d. dendriticum was studied. the highly basophilic germinative cells were found to differentiate into glycogen-containing parenchyma cells and three types of muscle cells. the cytodi-ferentiation was followed with the aid of 3h-thymidine. the differentiation potential of the germinative cells is discussed.1976997725
[the development and anatomy of diphyllobothrium dendriticum nitzsch 1824]. 195313137365
observations on the histogenesis of nervous tissue in diphyllobothrium dendriticum nitzsch, 1824 (cestoda, pseudophyllidea).the mode of growth of the populations of cells within and immediately surrounding the main lateral nerve cords in the actively growing, immature diphyllobothrium dendriticum was studied by the use of 3h-thymidine autoradiography. the population of nerve cells within the nerve cord grows only on account of cell migration from the surrounding parenchyma. no mitotic figures were observed in the nerve cords. the rate of growth is high. within a period of cultivation for 2 days in hamster 34% of the ...1976997724
egg size and form as taxonomic criteria in diphyllobothrium (cestoda, pseudophyllidea).the size and form (length, width, and length: width ratio) of eggs of diphyllobothrium dendriticum, d. ditremum and d. latum vary considerably among individual worms within each species. the size of eggs varies with host species and a decrease in egg size with increasing intensity of infestation is indicated. the egg size of d. latum increases during the first 10-12 days of egg production. for single worm infections in golden hamsters the mean egg length and width of d. ditremum are significantl ...1978652389
[fatty acid makeup of the cestodes, eubothrium crassum and diphyllobothrium dendriticum].the fat-acidic composition of e. crassum and d. dendriticum was investigated. lipids of e. crassum differ in greater unsaturation as compared to these of d. dendriticum as well as in greater amount of acids of type omega 3, whereas acids of type omega 6 and stearic acid were found in extracts of e. crassum in less quantity than in d. dendriticum. this phenomenon is characteristic of organisms living under conditions of low temperatures.1979492772
abnormal growth pattern of diphyllobothrium dendriticum in rainbow trout. 1979458130
effect of colchicine on dna synthesis in plerocercoids of diphyllobothrium dendriticum (cestoda). 19684172124
circadian rhythm of mitotic activity in the adult gull-tapeworm, diphyllobothrium dendriticum (cestoda). 19705475645
the excretory ducts of diphyllobothrium dendriticum (nitzsch 1824) plerocercoids: ultrastructure and marker distribution.an electron dense marker, lanthanum nitrate, was injected into the excretory ducts of living plerocercoids of diphyllobothrium dendriticum and the observations made by electron microscopy. the contractions of the plerocercoid spread the marker into the excretory system, and the distribution was found to be irregular both within the ducts and from one duct to another. communication between the smallest ducts and the rest of the excretory system was noted. the marker was also introduced from the s ...19836858310
activation of the peptidergic neurosecretory system in diphyllobothrium dendriticum (cestoda) at suboptimal temperatures. 19836858314
researches on pseudophyllidea (carus, 1813) in the south of chile. iv occurrence of diphyllobothrium dendriticum (nitzch).experimental infections were established with diphyllobothrium sp. plerocercoids obtained from salmo gairdneri, a fish introduced at the beginning of the 1900s in the lake region of chile. these permitted us to obtain adult parasites in larus dominicanus and canis familiaris. the histological features of the plerocercoids, their relation to the host, adult pattern and susceptibility of hosts allowed us to determine the presence of diphyllobothrium dendriticum (nitzch) for the first time in south ...19817276504
release of neurosecretory material by protrusions of bounding membranes extending through the axolemma, in diphyllobothrium dendriticum (cestoda).the release of neurosecretory material was studied in the cestode tapeworm diphyllobothrium dendriticum. in plerocercoid larvae cultivated in vitro the neurosecretory vesicles release their content by way of protrusions extending through the axolemma into the intercellular space. the released material is directed predominantly towards the muscles. no omega figures were detected. the significance of this new type of release is discussed.19817296642
[researches on pseudophyllidea (carus, 1813) in the south of chile. viii. experimental development of diphyllobothrium dendriticum nitzsch in larus maculipennis lichtenstein]. 19817347614
[the effect of host temperature on the fatty acid composition of the total and membrane lipids in the cestodes eubothrium crassum and diphyllobothrium dendriticum].a comparative qualitative and quantitative analysis of the fatty acid composition of total and lysosome membrane lipids of two cestode species eubothrium crassum (from cold-blooded host) and diphyllobothrium dendriticum (from warm-blooded host) has been carried out. it is shown that quantitative ratio of the fatty acids in the total and membranous lipids of the helminth of cold-blooded host was very similar whereas for the helminth of warm-blooded host fatty acid composition of membranous lipids ...19957483920
cloning, sequencing and characterization of an actin cdna in diphyllobothrium dendriticum (cestoda). 19947969276
parasites of the arctic fox (alopex lagopus) in iceland.forty-four of 50 arctic foxes (alopex lagopus) in iceland harbored 15 species of intestinal parasites, including protozoa: eimeria sp. or isospora sp. (in 4%); trematoda: cryptocotyle lingua (24%), plagiorchis elegans (4%), brachylaemus sp. (12%), tristriata sp. (10%), and spelotrema sp. (8%); cestoda: mesocestoides canislagopodis (72%), schistocephalus solidus (2%), and diphyllobothrium dendriticum (4%); nematoda: toxascaris leonina (50%), toxocara canis (2%), uncinaria stenocephala (4%), and e ...19938355346
immunocytochemical evidence for the presence of substance p-like peptide in diphyllobothrium dendriticum.substance p immunoreactivity (sp-ir) was detected in the nervous system of the gull-tapeworm diphyllobothrium dendriticum. the distribution of the sp-ir neurons in the plerocercoid differs from that of other peptidergic and aminergic neurons in the worm. as well as occurring in the ganglionic commissure and along the two main nerve cords, sp-ir neurons are located laterally to the main nerve cords but not dorsally or ventrally. the sp-ir neurons have projections extending to the surface. bipolar ...19938479806
relationships in the parasite-host system as exemplified by the diphyllobothrium dendriticum (cestoda: diphyllobothriidae)-herring gull larus argentatus. 201526335966
[cytomorphological and biochemical characteristics of the whitefish, baikal omul coregonus migratorius, infected by plerocercoids of diphyllobothrium dendriticum (cestoda: pseudophyllidae)].the cytomorphological and biochemical composition of the blood has been studied for the deep-dwelling (bottom) morphotype of the baikal omul infected by plerocercoids of diphyllobothrium dendriticum, during the fish spawning migration. a decrease in hemoglobin synthesis and an increase in the proliferative activity of erythroid cells have been registered. the changes in the leukocyte population composition, immunoglobulin, and total protein concentrations evidenced a disorder of blood cell proli ...201626021157
diphyllobothrium dendriticum and diphyllobothrium latum in fishes from southern argentina: association, abundance, distribution, pathological effects, and risk of human infection.diphyllobothrium dendriticum and diphyllobothrium latum are reported for the first time from argentina. the following species from lake moreno (southern argentina) were studied to determine whether plerocercoids were present: 11 brook trout (salvelinus fontinalis), 32 perca (percichthys sp.), 21 pejerrey (patagonina hatcheri), and 114 rainbow trout (oncorhynchus mykiss). brook trout harbored both species (27.3% prevalence of d. dendriticum and 9.0% of d. latum); perca harbored only d. latum (18. ...19938501594
gastrointestinal helminths of arctic foxes (alopex lagopus) from different bioclimatological regions in greenland.nine species of gastrointestinal helminths were recovered from 254 arctic foxes (alopex lagopus) from 8 different localities in greenland. prevalences of infection with the helminth species differed from area to area: toxascaris leonina (39-68%), strongyloides stercoralis (0-14%), mesocestoides lineatus (0-58%), diphyllobothrium dendriticum (0-15%), taenia ovis krabbei (0-70%), cryptocotyle sp. (0-3%), plagiorchis elegans (0-6%), and polymorphus sp. (0-3%). additionally, a taenia species, which ...19968627489
isolation and characterization of five actin cdnas from the cestode diphyllobothrium dendriticum: a phylogenetic study of the multigene family.five cdnas (pdidact2-pdidact6), representing different actin genes, were isolated from a diphyllobothrium dendriticum cdna library, and the dna as well as the putative amino acid sequences were determined. the corresponding didact2 and didact4 genes code for peptides 376 amino acids long, with molecular weights 41,772 and 41,744 da, respectively, while the deduced didact3 protein is 377 amino acids long and weighs 41,912 da. the pdidact5 and -6 cdnas lack nucleotides corresponding to three to si ...19979069176
three main patterns in the expression of six actin genes in the plerocercoid and adult diphyllobothrium dendriticum tapeworm (cestoda).the expression of six actin genes was examined in adult and plerocercoid diphyllobothrium dendriticum tapeworms using in situ hybridization. on the basis of their structures, these genes are divided into three groups, the cestoda-i, -ii and -iii actins. current studies show that the expression of actins belonging to different groups vary to a great extent. the three cestoda-i actins are expressed primarily in muscle cells of both adult and plerocercoid tapeworms, the expression being restricted ...19979200126
infection of brown trout with diphyllobothrium dendriticum procercoids.the aims of this experimental study were to develop a practical method of controlling the number of diphyllobothrium dendriticum procercoids delivered to a fish host; to examine the effect of different procercoid doses (3, 7 and 15) on the plerocercoid level in fish; and to examine the potential mortality caused by plerocercoids. the experiment was terminated after 2 months. the prevalence of infection at the lowest dose level, 17.2%, was statistically significantly lower than at medium and high ...19979421717
the distribution of f-actin during the development of diphyllobothrium dendriticum (cestoda).the distribution of actin filaments in all developmental stages of the tapeworm diphyllobothrium dendriticum was studied. it is the first investigation of the placement of microfilaments during the development of a flatworm, and the results show that actin filaments, in all developmental stages, can be found in the subtegument and the flame cells. muscle fibers possibly corresponding to the longitudinal, transversal, and dorsoventral muscles of the adult and plerocercoid were already detected in ...19989477312
the ecology of endoparasitic helminth infections of brown trout (salmo trutta) and rainbow trout (oncorhynchus mykiss) in scotland.two hundred and forty brown trout (salmo trutta) and 49 rainbow trout (oncorhynchus mykiss), obtained from 21 locations in central scotland between october 1990 and august 1993, were examined for endoparasitic helminth infections. crepidostomum farionis (digenea) was the most widely distributed helminth species, followed by eubothrium crassum (cestoda), diphyllobothrium dendriticum and d. ditremum (cestoda), neoechinorhynchus rutili (acanthocephala), echinorhynchus truttae (acanthocephala), eust ...19959599425
the nadph-diaphorase staining reaction in relation to the aminergic and peptidergic nervous system and the musculature of adult diphyllobothrium dentriticum.a new category of signalling molecules--transmitter gases--has appeared. nitric oxide (no) is generated by nitric oxide synthase (nos) and diffuses as a short-lived transcellular messenger through the plasma membrane. nadph-diaphorase (nadph-d) is a marker enzyme for no-producing neurons. in this study the pattern of nadph-d stained neurons in diphyllobothrium dendriticum is described and compared to the pattern of aminergic and peptidergic neuronal elements and to that of the musculature staine ...19989774794
temperature effects on embryonic development and the life cycle of diphyllobothrium dendriticum.diphyllobothrium dendriticum eggs collected from hamster faeces were incubated at 10 or 20 degrees c, or maintained at 4 degrees c for 11-30 months. on day 65, 20-50% of eggs failed to hatch at 10 degrees c and 42-51% did not hatch by day 21 at 20 degrees c. our study indicates that eggs begin to hatch in mid-august and persist until october in many lakes within the natural range of d. dendriticum. our results demonstrate that eggs stored at 4 degrees c will hatch, suggesting they can persist in ...200010899530
aggregation of helminths: the role of feeding behavior of fish hosts.individual arctic charr (salvelinus alpinus) from fjellfrøsvatn, northern norway, could be categorized by their stomach contents as zooplanktivores or benthivores. feeding specialization among these fish was evident from negative correlations between helminths transmitted by pelagic copepods (diphyllobothrium dendriticum and d. ditremum) and those transmitted by the benthic amphipod gammarus lacustris (cystidicola farionis and cyathocephalus truncatus). occurrences of parasite species acquired f ...200415040660
inter- and intra-specific characterization of tapeworms of the genus diphyllobothrium (cestoda: diphyllobothriidea) from switzerland, using nuclear and mitochondrial dna targets.human diphyllobothriosis is caused by at least 14 species of cestodes belonging to the genus diphyllobothrium. molecular analysis by sequencing of nuclear and mitochondrial targets identifies some species at inter- and intra-specific level, and helps to reconstruct their phylogenetic relationships. nevertheless, the suitability of further molecular targets deserves to be widened, and the comparison of samples of different geographical origin could allow their intra-specific characterization, whi ...201019800982
[morphofunctional characteristics of thymus and immunological parameters in golden hamster under experimental invasion with the cestode diphyllobothrium dendriticum].morphofunctional changes induced by the tapeworm in the thymus and blood of its definitive host, by an example of the experimental model "diphyllobothrium dendriticum--mesocricetus auratus", have been investigated. the morphofunctional changes in the thymus and immunological transformations in the organism of infested hamster are evidences of rather high immunogenic effect of the d. dendriticum antigens on the definitive host at the period of active growth of the parasites.201020536005
[changes in mast cell composition in the hamster duodenum in experimental diphyllobothriosis].the morphometric investigation was performed to study the reaction of mast cells (mc) in mucosal lamina propria of hamster duodenum to experimental infection with tapeworm diphyllobothrium dendriticum. the invasion was found to result in a significant increase of mc cells absolute content, changes in the proportions of their functional groups, increased anaphylactic mc degranulation, enhanced mc precursor migration and their accelerated differentiation.201020572394
[the reaction of the bursa and thymus eosinophils in the herring gull after the experimental infection with gull-tapeworm].using the methods of light microscopy, eosinophil topography, quantitative and qualitative changes (degranulation level of and a cationic protein content) were studied in the thymus and bursa of 36 herring gull nestlings larus argentatus mongolicus (suskin, 1925) 2 weeks after experimental infestation with gull-tapeworm diphyllobothrium dendriticum (nitsch, 1824). eosinophils in the thymus were located in trabecules, mainly close to the blood vessels, thymic (hassall's) corpuscles and also direc ...201021500430
the tapeworm diphyllobothrium dendriticum (cestoda) produces prostaglandin e(2), a regulator of host immunity. 201122227682
rapid identification of nine species of diphyllobothriidean tapeworms by pyrosequencing.the identification of diphyllobothriidean tapeworms (cestoda: diphyllobothriidea) that infect humans and intermediate/paratenic hosts is extremely difficult due to their morphological similarities, particularly in the case of diphyllobothrium and spirometra species. a pyrosequencing method for the molecular identification of pathogenic agents has recently been developed, but as of yet there have been no reports of pyrosequencing approaches that are able to discriminate among diphyllobothriidean ...201627853295
parasitation of sea trout (salmo trutta trutta l.) from the spawning ground and german coastal waters off mecklenburg-western pomerania, baltic sea.a total of 52 sea trouts, salmo trutta trutta, were studied for parasites, originating from german freshwater streams and coastal waters of the baltic sea. while 35 specimens were caught mainly close to the shoreline in the baltic sea, 17 were sampled during their spawning migration in warnow river and other neighboring rivers in mecklenburg-western pomerania. a total of 12 different metazoan parasite species were found in sea trout originating from the baltic sea, including five digeneans, two ...201626374539
gaba in the nervous system of the cestodes diphyllobothrium dendriticum (diphyllobothriidea) and caryophyllaeus laticeps (caryophyllidea), with comparative analysis of muscle innervation.the nervous system (ns) of the cestodes diphyllobothrium dendriticum (diphyllobothriidea) and caryophyllaeus laticeps (caryophyllidea) was investigated using immunocytochemistry. the gaba neurotransmitter was identified in the ns of both species; gabaergic neurons were detected in the main nerve cords (mc). gaba-like immunoreactive neurons were predominantly unipolar and exhibited more intensive immunoreactivity in the neurite than in the perikaryon. in c. laticeps , gaba-like immunoreactive som ...201424641216
prostaglandins e2 and d2-regulators of host immunity in the model parasite diphyllobothrium dendriticum: an immunocytochemical and biochemical study.the spectrum of immunomodulating molecules produced by tapeworms is not yet well understood. the aims of this study, on the tapeworm diphyllobothrium dendriticum, were: 1) detection and quantification of prostaglandins (pgs) e2 and d2 by high performance liquid chromatography; 2) visualization of pge2 and pgd2 in specific cells, using methods of immunocytochemistry and confocal laser scanning microscopy; and 3) investigation of the ultrastructure of the cells potentially producing pge2 and pgd2. ...201728137629
[localization of prostaglandin e2, γ-aminobutyric acid, and other potential immunomodulators in the plerocercoid diphyllobothrium dendriticum (cestoda)].for the first time, the potential immunomodulators prostaglandin e2 and γ-aminobutyric acid (gaba) have been revealed in the plerocercoid diphyllobothrium dendriticum, which is a parasite in the tissues and abdominal cavity of the baikal omul coregonus migratorius. the localization of immunomodulators in parasite tissues was compared with the location of typical markers of the nervous system (serotonin (5-ht) and fmrf-amide) and a marker of microtubules (α-tubulin). prostaglandin e2 was revealed ...201625731038
parasite communities of two three-spined stickleback populations in subarctic norway--effects of a small spatial-scale host introduction.co-introduction and colonization of parasites with the introduction of new host species into aquatic habitats may depend on the host specificity and dispersal capabilities of the parasites. we compared the metazoan parasite community of an introduced three-spined stickleback (gasterosteus aculeatus) population with that of the nearby source population in subarctic norway. as expected from a small spatial scale (5 km), the parasite component communities in the two lakes were highly similar. all i ...201525630694
the influence of changing prey availability on the prevalence of diphyllobothrium in river otters from yellowstone national park.parasite prevalence in predatory mammals is influenced by numerous factors including diet, sex, season, and habitat. we examined the effect of such factors on the prevalence of gastrointestinal helminths in north american river otters ( lontra canadensis ) in yellowstone lake, yellowstone national park. otters in this ecosystem have recently experienced a decline in their main prey, yellowstone cutthroat trout ( oncorhynchus clarkii bouvieri), and have, in turn, increased consumption of alternat ...201525192057
tapeworm diphyllobothrium dendriticum (cestoda)--neglected or emerging human parasite?a total number of 14 valid species of diphyllobothrium tapeworms have been described in literature to be capable of causing diphyllobothriosis, with d. latum being the major causative agent of all human infections. however, recent data indicate that some of these infections, especially when diagnosed solely on the basis of morphology, have been identified with this causative agent incorrectly, confusing other diphyllobothrium species with d. latum. another widely distributed species, d. dendriti ...201324386497
imported diphyllobothriasis in switzerland: molecular methods to define a clinical case of diphyllobothrium infection as diphyllobothrium dendriticum, august 2010.following a first clinical case of infection by diphyllobothrium dendriticum in switzerland in 2006, we report a second case in the country. the species was identified by molecular methods. in the swiss, french and italian subalpine regions, human diphyllobothriasis has seen a comeback since the late 1980's, and diphyllobothrium latum is usually considered the causative agent of the disease. in addition, several locally acquired and imported clinical infections due to allochthonous diphyllobothr ...201323351654
prevalence, intensity, and abundance of infection and pathogenesis caused by diphyllobothriosis in vulnerable, native fish and introduced trout in lake panguipulli, chile.diphyllobothriosis in fish from freshwater ecosystems in southern chile was first reported in 1949. infection by plerocercoids of diphyllobothrium latum and diphyllobothrium dendriticum occurs in introduced trout (oncorhynchus mykiss) and native fish. we determined the prevalence, mean intensity, and mean abundance of seasonal infection and tissue damage produced by diphyllobothrium spp. in native fish (percichthys trucha, odontesthes mauleanum, and basilichthys australis) and introduced trout ( ...201223060495
[the environmental conditions and predictors of formation of the bases for a combination of diphyllobothrium dendriticum and triaenophorus nodulosus foci in the flood-river ecosystem of the angara river].investigations were conducted in the context of the landscape-biocenological concept. the formation of water-storage basins in the valley of the angara river was shown to give rise to the transformation of the morphological structure and hydrological regimen of a landscape, which determined a change in some environmental conditions and factors of a combination of foci and the emergence of others. the morphological structure and hydrological regimen of a landscape, the species composition of ecos ...201222774497
[morpho-functional changes of cloacal bursa of the herring gull in experimental infection with gull tapeworm].using the methods of light microscopy, the quantitative changes in the cells of the cloacal bursa of herring gull (larus argentatus mongolicus) chicks were studied during experimental infection with gull-tapeworm diphyllobothrium dendriticum. the area of the follicles within the bursa and the number of eosinophils in the infected chicks were found to increase. in the follicles the number of blast cells and large lymphocytes was elevated. the reduction of small lymphocytes and plasma cells number ...201222724332
first molecular identification of diphyllobothrium dendriticum plerocercoids from feral rainbow trout (oncorhynchus mykiss) in chile.between april and june 2009, 1,075 feral rainbow trout from 10 different lakes involved with aquaculture activities in los lagos region, chile, were inspected for diphyllobothrium species. all viscera and muscles of the fish were examined using stereomicroscopy; pyloric cecae and stomachs infected with plerocercoids were checked by histology and scanning electron microscopy. plerocercoids of diphyllobothrium dendriticum were confirmed by pcr and sequencing of coi and 18s rrna + its1 + 5.8s rrna ...201222715967
validity of the bear tapeworm diphyllobothrium ursi (cestoda: diphyllobothriidae) based on morphological and molecular markers.the bear tapeworm diphyllobothrium ursi is described based upon the morphology of adult tapeworms recovered from the brown bear (ursus arctos middendorffi) and larval plerocercoids found in sockeye salmon (oncorhynchus nerka) from kodiak island in alaska in 1952. however, in 1987 d. ursi was synonymized with diphyllobothrium dendriticum, and the taxonomic relationship between both species has not subsequently been revised. in this study mitochondrial cytochrome c oxidase subunit 1 gene (cox1) se ...201222663179
growth, nutritional composition, and hematology of arctic charr (salvelinus alpinus) exposed to toxaphene and tapeworm (diphyllobothrium dendriticum) larvae.toxaphene, an organochlorine pesticide, is the major contaminant of arctic charr (salvelinus alpinus) in the canadian arctic. the objective of this study was to investigate the combined effects of toxaphene exposure and infection by the larval stage of the cestode diphyllobothrium dendriticum on fish growth, nutritional composition, and hematology. hatchery-reared arctic charr were subjected to one of four treatments: (1) oral administration of corn oil (control); (2) single oral dose of 10 micr ...200515719195
selected pathological, immunohistochemical and ultrastructural changes associated with an infection by diphyllobothrium dendriticum (nitzsch, 1824) (cestoda) plerocercoids in coregonus lavaretus (l.) (coregonidae).the pathological changes induced by an infection of diphyllobothrium dendriticum (nitzsch, 1824) plerocercoids in powan, coregonus lavaretus (l.), from loch lomond, scotland, were assessed using immunohistochemical and ultrastructural techniques. in a sample of 26 powan, the occurrence of encysted plerocercoids of d. dendriticum on the outer surface of the stomach was 38.5% (n = 10) with the number of cysts ranging from 4 to 15 and measuring 4.2 +/- 1.0 mm x 3.4 +/- 0.9 mm (mean +/- sd). histolo ...200717640250
imported diphyllobothriasis in switzerland: molecular evidence of diphyllobothrium dendriticum (nitsch, 1824).we report for the first time in switzerland a clinical case because of diphyllobothrium dendriticum, identified by molecular methods. we discuss the potential for this imported species to infect local intermediate hosts and thus to achieve autochthonous cyclic transmission.200817805571
[hematological and immunological properties of herring gull (larus argentatus) nestlings experimentally infected with diphyllobothrium dendriticum (cestoda: pseudophyllidae)].the morphological structure of the blood in herring gull nestlings and their immune system response to experimental infection with tapeworm diphyllobothrium dendriticum have been studied. the leukocyte reaction pattern and the dynamics of immunoglobulin synthesis and populations of immunocompetent cells in the blood of nestlings during early invasion indicated a short duration of the cellular immune response and an insignificant humoral immune response. a pronounced trend to decrease in the hemo ...200717966904
old problems on a new playing field: helminth zoonoses transmitted among dogs, wildlife, and people in a changing northern climate.although surveillance is limited, indigenous residents at latitudes ranging from 53 to 73°n in canada appear to have a higher occurrence of infection with some zoonotic parasites than the general population. conversely, they are relatively naïve to other zoonotic parasites that have previously been unable to establish at northern latitudes. for those parasites that circulate among dogs, wildlife, and people, potential risk factors in the north include limited availability of veterinary services, ...201121802208
[hematology and blood chemistry of the herring gull larus argentatus infested by cestodes diphyllobothrium dendriticum (cestoda: diphyllobothriidae)].hematological and plasma biochemical analyses and parasitological detection were carried out in the herring gull larus argentatus. hematological and plasma biochemical parameters in uninfected gulls and gulls infected by diphyllobothrium dendriticum (cestoda: diphyllobothriidae) were compared. hemoglobin content and numbers of erythrocytes were reduced; numbers of leukocytes and sorption capacity of erythrocytes were improved in blood of the infected herring gulls. hematological index (eosinophi ...201729211426
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