| replication of four aquatic reoviruses in experimentally infected golden shiners (notemigonus crysoleucas). | golden shiners (notemigonus crysoleucas) experimentally infected with four reoviruses supported replication of golden shiner virus as well as chum salmon virus, reovirus 13p2 and catfish reovirus at temperatures of 23 and 28 c. all four reoviruses replicated in golden shiners in this study. natural infections of the golden shiner virus are known only in golden shiners. | 1991 | 1656105 |
| fish cell lines: establishment and characterization of three new cell lines from grass carp (ctenopharyngodon idella). | three new cell lines were established from tissues of the grass carp, ctenopharyngodon idella. derived from the fin, snout, and swim bladder of two apparently healthy diploid fry, these cell lines have been designated gcf, gcs-2, and gcsb, respectively. the cells grew at temperatures between 24 degrees and 36 degrees c with optimal growth at 32 degrees c and have been subcultured more than 50 times since their initiation in august 1986. two of the lines remained diploid or pseudodiploid after 38 ... | 1990 | 2108122 |
| comparative activity of gill atpase in three freshwater teleosts exposed to cadmium. | the comparative activity of gill atpase was examined in the bluegill sunfish (lepomis macrochirus), fathead minnow (pimephales promelas), and golden shiner (notemigonus crysoleucas). basal na/k atpase activity was highest in bluegill sunfish (1.46 mumol pi/mg protein/hr) and lowest in golden shiners (1.01 mumol pi/mg protein/hr). while a stimulation of na/k atpase activity was observed at an exposure concentration of 1 micrograms cd/liter in the bluegill sunfish and fathead minnows, an inhibitio ... | 1987 | 2826104 |
| morphological and biochemical properties of four members of a novel group of reoviruses isolated from aquatic animals. | the morphological, biochemical and growth characteristics of four members of the reoviridae, three from the fish hosts, golden shiner (notemigonus crysoleucas), chum salmon (oncorhynchus keta) and channel catfish (ictalurus punctatus) and one from american oyster (crassostrea virginica), were compared. electron microscopy of negatively stained virions revealed icosahedral particles approximately 75 nm in diameter composed of a double capsid. complete particles had buoyant densities in cscl of 1. ... | 1987 | 3819694 |
| non-specific cytotoxic activity of teleost leukocytes. | the existence of lymphoid cells with "natural" killer activity in mammals and birds has been known for some time. several previous reports have demonstrated similar activity in carp and catfish kidney leukocytes. however, the activity previously reported was directed towards established mammalian cell lines. in this report we confirm the existence of spontaneous killer activity in other species of teleosts, including salmonids. this spontaneous cytotoxic activity is directed towards several esta ... | 1985 | 3996708 |
| fish viruses serologic comparison of the golden shiner and infectious pancreatic necrosis viruses. | high titer neutralizing antiserum to golden shiner virus (gsv) was produced in a rabbit. the gsv antiserum had significant neutralization indices in tests with the original strain of gsv and with another more recent isolate. neutralization indices were not significant when two strains of infectious pancreatic necrosis virus (ipnv) were reacted with gsv antiserum, and neither isolate of gsv showed significant reactivity in neutralization tests with polyvalent ipnv antiserum. our results indicate ... | 1980 | 6162037 |
| characteristics of a new reovirus from channel catfish (ictalurus punctatus). | the characteristics of a reovirus (crv) recently isolated from channel catfish (ictalurus punctatus) were examined following purification of virions from infected cell cultures. virions had double capsids, the inner and outer with diameters of 55 nm and 75 nm respectively. complete virions had a density of 1.36 g/ml in cscl gradients and contained seven polypeptides of 132 000, 130 000, 110 000, 68 000, 56 000, 43 000 and 32 000 mol. wt. the nucleic acid labelled with [5-3h]uridine had a density ... | 1984 | 6470683 |
| population biology and host-parasite relationships of triganodistomum attenuatum (trematoda: lissorchiidae) infecting the white sucker, catostomus commersoni (lacépède). | triganodistomum attenuatum demonstrated similar seasonal infection patterns in white suckers, catostomus commersoni, from the bellamy and oyster rivers, new hampshire. mean intensity was highest in spring 1975 and 1976. nongravid and gravid worms were common in the spring, and in the spring and summer, respectively. most individuals of t. attenuatum parasitized the posterior portion of the sucker's intestine. no definite changes in prevalence and mean intensity were evident at either river as fi ... | 1980 | 7391871 |
| fish shoal composition: mechanisms and constraints. | observations were made on three fish species (banded killifish (fundulus diaphanus), golden shiner (notemigonus crysoleucas) and white sucker (catostomus commersoni)) in a temperate lake (new brunswick, canada) in order to investigate the relationship between shoal choice behaviour of individual fishes and shoal composition. encounters between shoals were observed to take place every 1.1 min per shoal and an encounter lasted 3.7 s on average. the duration of shoal encounters was influenced by sh ... | 2000 | 11075715 |
| common evolutionary origin of aquareoviruses and orthoreoviruses revealed by genome characterization of golden shiner reovirus, grass carp reovirus, striped bass reovirus and golden ide reovirus (genus aquareovirus, family reoviridae). | full-length and partial genome sequences of four members of the genus aquareovirus, family reoviridae (golden shiner reovirus, grass carp reovirus, striped bass reovirus and golden ide reovirus) were characterized. based on sequence comparison, the unclassified grass carp reovirus was shown to be a member of the species aquareovirus c. the status of golden ide reovirus, another unclassified aquareovirus, was also examined. sequence analysis showed that it did not belong to the species aquareovir ... | 2002 | 12124458 |
| mercury effects on predator avoidance behavior of a forage fish, golden shiner (notemigonus crysoleucas). | mercury contamination of fish is widespread in north america and has resulted in the establishment of fish consumption advisories to protect human health. however, the effects of mercury exposure to fish have seldom been investigated. we examined the effects of dietary mercury exposure at environmental levels in a common forage species, golden shiner (notemigonus crysoleucas). fish were fed either an unaltered diet (12 ng/g wet wt methylmercury [mehg] as hg), a low-hg diet (455 ng/g hg), or a hi ... | 2003 | 12836981 |
| morphological and genetic characteristics of flavobacterium columnare isolates: correlations with virulence in fish. | variability in pathogenicity of flavobacterium columnare makes disease treatment difficult because there is currently no way to easily recognize those strains that warrant aggressive treatments. in order to identify suitable virulence markers, 17 isolates of f. columnare were cultured from six different fish species. the dna from all isolates was analysed using random amplified polymorphic dna (rapd). bootstrap analysis of the rapd data produced a tree with three major groups supported by bootst ... | 2004 | 14986937 |
| molecular cloning, dna sequence analysis, and expression of cdna sequence of rna genomic segment 6 (s6) that encodes a viral outer capsid protein of threadfin aquareovirus (tfv). | the genome segment 6 (s6) of threadfin reovirus (tfv) was cloned and sequenced. the entire s6 nucleotide sequence is 2056 bp long with an open reading frame that encodes a protein of 653 amino acids. sequence analysis of the tfv s6 genome revealed that the 5'-terminal sequence, gtttta and the 3'-terminal sequence, attcatc of the plus strand is common to other genome segments of tfv. the pentanucleotide, tcatc, at the 3'-terminal of the plus strand was also conserved in other reported isolates of ... | 2005 | 15744578 |
| acute toxicity of isopropyl methylphosphonic acid, a breakdown product of sarin, to eggs and fry of golden shiner and channel catfish. | several countries, including the united states, have agreed to destroy stockpiled chemical warfare agents in accordance with the chemical weapons convention treaty of 1993. sarin is one of many chemical warfare agents (cwa) designated for destruction. in the event of an accident during incineration, sarin or its decomposition products have the potential to be expelled into the environment. sarin hydrolyzes into isopropyl methylphosphonic acid (impa), a compound detected in groundwater from prior ... | 2005 | 15762552 |
| taxonomy and molecular phylogeny of myxobolus bilobus n. sp. (myxozoa) parasitizing notemigonus crysoleucas (cyprinidae) in algonquin park, ontario, canada. | myxobolus bilobus n. sp. (myxozoa) is described from golden shiner notemigonus crysoleucas in algonquin park, ontario, canada. plasmodia develop at the distal end of gill filaments, where they form a novel-shaped plasmodium made up of 2 side-by-side hemispheres joined at a central pore. surrounding gill tissue is vacuolated, necrotic, and hemorrhagic. spores of m. bilobus n. sp. resemble those of m. aureatus ward, 1919, m. angustus kudo, 1934, m. spalli landsberg & lom, 1991, and m. pseudokoi li ... | 2005 | 16261938 |
| morphological, developmental and ecological evidence for a progenetic life cycle in neochasmus (digenea). | seven species of fishes, catostomus commersonii (lacépède), etheostoma nigrum rafinesque, micropterus dolomieu lacépède, notemigonus crysoleucas (mitchill), notropis hudsonius (clinton), perca flavescens (mitchill), and percina caprodes (rafinesque) from the st. lawrence river, quebec, canada, were found infected with progenetic specimens of neochasmus spp. in the orbits and/or the body musculature. worms displayed varying degrees of maturation. eggs occupied the entirety of the worm in late sta ... | 2006 | 16696430 |
| [(3)h] citalopram binding to serotonin transporter sites in minnow brains. | mammalian serotonin (sert) and norepinephrine transporters (net) are target sites for antidepressants and are affected by pesticide exposures. herein, we examined whether golden shiner (notemigonus crysoleucas) or fathead minnow (pimphales promelas) serts and catecholamine transporters respond comparably to mammalian serts and nets. we compared the pharmacological profiles of central sert and net binding sites of the golden shiner minnow to those of rats. homogenate binding with the radioligand ... | 2007 | 17697042 |
| validation of a quantitative pcr diagnostic method for detection of the microsporidian ovipleistophora ovariae in the cyprinid fish notemigonus crysoleucas. | microsporidian parasites are easily detected by light microscopy when infections are heavy and spores are present. however, early infections without spores, or light infections with low numbers of spores, are easily missed. this limitation has made it difficult to conduct investigations into microsporidian prevalence and transmission. in this study, we developed a quantitative taqman polymerase chain reaction assay to assess the presence of ovipleistophora ovariae in the tissues of the cyprinid ... | 2007 | 17803107 |
| phylogenetic affinities of plagiocirrus van cleave and mueller, 1932 with the description of a new species from the pascagoula river, mississippi. | plagiocirrus loboides n. sp. (digenea: opecoelidae) is described from fundulus nottii, f. dispar blairae, f. chrysotus, and notemigonus crysoleucas from the pascagoula river in mississippi. plagiocirrus loboides differs from p. primus van cleave and mueller, 1932, by having a longer postcecal space (14-25% of body length vs. about 7%); a more anterior vitellarium (extending at least to the middle of the ventral sucker vs. to its posterior margin); and larger eggs (51-71 microm long by 23-34 micr ... | 2007 | 18314693 |
| evaluation of dairy-yeast prebiotic supplementation in the diet of juvenile goldfish in the presence or absence of phytoplankton and zooplankton. | prebiotics recently have been shown to increase immune responses and disease resistance in certain fish species; therefore, the current study was conducted to evaluate the commercially available dairy-yeast prebiotic, grobiotic-a, for use with juvenile goldfish carassius auratus. the study consisted of two 10-week feeding trials in which juvenile goldfish were fed practical diets that were either unsupplemented or supplemented with the dairy-yeast prebiotic at 2% by dry weight. juvenile fish wer ... | 2009 | 20043400 |
| detection and prevalence of the nonsyncytial american grass carp reovirus aquareovirus g by quantitative reverse transcriptase polymerase chain reaction. | the american grass carp reovirus (agcrv) aquareovirus g is not strongly associated with disease in fish, but it is often detected by cell culture during routine inspections of healthy fish. the cytopathic effect of agcrv does not involve the typical syncytia associated with most aquareoviruses. instead, the agcrv produces a pattern of cell rounding that is very similar to that produced by rhabdoviruses, including those that are highly regulated. we have developed a quantitative polymerase chain ... | 2010 | 20575360 |
| linking larvae and adults of apharyngostrigea cornu, hysteromorpha triloba and alaria mustelae (diplostomoidea: digenea) using molecular data. | abstract because the taxonomy of trematodes is based on adults, the larval stages of most digeneans cannot be identified to species based on morphology alone. molecular data provide a means of linking larval stages to known adults. we obtained sequences from the barcode region of cytochrome oxidase i (co1) from adult and larval parasites of fish, frogs, birds, and mammals across north america. sequences from adult apharyngostrigea cornu, hysteromorpha triloba, and alaria mustelae (diplostomoidea ... | 2011 | 21510747 |
| isolation and characterization of a novel reovirus from white bream blicca bjoerkna. | during a fish health inspection in the viennese waterway 'old danube', a virus was isolated exclusively from white bream blicca bjoerkna (l.) (formerly abramis bjoerkna l.), one of the most abundant cyprinids present and not known as a host species for this virus. the virus preferentially replicated in cultures of the epithelioma papulosum cyprini cell line where focal plaques of infection developed slowly. examination of infected cell cultures by electron microscopy revealed non-enveloped 60 to ... | 2014 | 25449324 |
| fin and gill biopsies are effective nonlethal samples for detection of viral hemorrhagic septicemia virus genotype ivb. | nonlethal sampling is becoming a common method to diagnose fish diseases, especially with the availability of molecular testing. viral hemorrhagic septicemia virus (vhsv) is a viral pathogen of finfish distributed worldwide. although vhsv has been known to occur in some parts of the world for decades, a new genotype, ivb, recently emerged in the laurentian great lakes of northeastern north america. golden shiners (notemigonus crysoleucas; mitchill, 1814) and fathead minnows (pimephales promelas; ... | 2013 | 23404480 |
| general and histological indicators of health in wild fishes from a biological mercury hotspot in northeastern north america. | kejimkujik national park and national historic site, nova scotia, canada, is considered a biological mercury (hg) hotspot because the tissues of yellow perch (perca flavescens) and common loons (gavia immer) inhabiting the lakes frequently exceed so-called safe levels of hg. in the present study, the relationships between hg and overall health of males and females of 3 forage fish species (brown bullhead ameirus nebulosus, banded killifish fundulus diaphanus, and golden shiner notemigonus crysol ... | 2017 | 27595668 |
| molecular detection of a novel totivirus from golden shiner (notemigonus crysoleucas) baitfish in the usa. | during regulatory and routine surveillance sampling of apparently healthy baitfish from the state of minnesota, a novel totivirus (tentatively named "golden shiner totivirus", gstv) was detected in a homogenate of kidney and spleen of golden shiner (notemigonus crysoleucas). the nearly complete genome is 7788 nt long with a complete 5' untranslated region (utr) of 135 nt (1-135 nt position), complete open reading frames (orfs) and a partial 3' utr of 54 nt (7734-7788). the sequence is comprised ... | 2016 | 27231008 |
| vision in two cyprinid fish: implications for collective behavior. | many species of fish rely on their visual systems to interact with conspecifics and these interactions can lead to collective behavior. individual-based models have been used to predict collective interactions; however, these models generally make simplistic assumptions about the sensory systems that are applied without proper empirical testing to different species. this could limit our ability to predict (and test empirically) collective behavior in species with very different sensory requireme ... | 2015 | 26290783 |