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amino acid sequence of bumblebee mcd peptide: a new mast cell degranulating peptide from the venom of the bumblebee megabombus pennsylvanicus.a new 28 amino acid peptide, we recently isolated from the venom of the bumblebee megabombus pennsylvanicus. has been characterized. the peptide, met-cys-ile-cys-lys-asn-gly-lys-pro-leu-pro-gly-phe-ile-gly-lys-ile-cys- arg-lys-ile-cys-met-met-gln-gln-thr-his(nh2), has been named bumblebee mast cell degranulating (mcd) peptide due to its ability to degranulate rat peritoneal mast cells, and its resemblance to the bee venom mcd peptide. bumblebee mcd peptide, unlike bombolitins, the other mast cel ...19852421265
bombolitins, a new class of mast cell degranulating peptides from the venom of the bumblebee megabombus pennsylvanicus.five structurally related heptadecapeptides rich in hydrophobic amino acids have been discovered in the venom of the bumblebee megabombus pennsylvanicus. we have named them bombolitin i (ile-lys-ile-thr-thr-met-leu-ala-lys-leu-gly-lys-val-leu-ala-his-val-nh2 ), bombolitin ii (ser-lys-ile-thr-asp-ile-leu-ala-lys-leu-gly-lys-val-leu-ala-his-val-nh2 ), bombolitin iii (ile-lys-ile-met-asp-ile-leu-ala-lys-leu-gly-lys-val-leu-ala-his-val-nh2 ), bombolitin iv (ile-asn-ile-lys-asp-ile-leu-ala-lys-leu-va ...19852578459
phenology of bombus pennsylvanicus sonorus say (hymenoptera: apidae) in central mexico.we studied the seasonal activity of bombus pennsylvanicus sonorus say in subtropical conditions of the mexican central plateau. monthly during 1998, we recorded caste of active individuals (inferred form corporal size measured as wing length), presence and activity of reproductive individuals, and mating activity. also, we recorded the flower plants used as resources. subtropical conditions of the mexican central plateau do not seem to modify phenological features of b. pennsylvanicus sonorus si ...200617144129
comparative genetic analyses of historical and contemporary collections highlight contrasting demographic histories for the bumble bees bombus pensylvanicus and b. impatiens in illinois.direct comparison of genetic patterns between museum specimens and contemporary collections can be a powerful approach for detecting recent demographic changes. using microsatellite markers, we examined historical and contemporary genetic variation from an apparently declining bumble bee species, bombus pensylvanicus, and from a stable species, bombus impatiens, in central illinois. for each species, we genotyped specimens from the illinois natural history survey collected from three populations ...200919344350
geographic patterns in the reproductive ecology of agave lechuguilla (agavaceae) in the chihuahuan desert. i. floral characteristics, visitors, and fecundity.floral characteristics such as morphology and flower color have been interpreted as adaptive traits that evolved through selective pressures generated by pollinators. differences among populations in the expression of floral characters could result from natural selection for their adaptive value to local conditions. we describe the patterns of variation of flower morphology, color, and fecundity of agave lechuguilla in 11 populations along a latitudinal gradient encompassing the whole range of t ...200321659130
Purification and inflammatory edema induced by two PLA(2) (Anch TX-I and Anch TX-II) from sea anemone Anthothoe chilensis (Actiniaria: Sagartiidae).The Anch TX-I and II PLA(2) were purified from Anthothoe chilensis (Lesson, 1830) from the extract of the anemone after only two chromatographic step using molecular exclusion chromatography (Sephadex G-75) and reverse phase HPLC on µ-Bondapak C18 column. Both PLA(2) showed a molecular mass of ~14kDa determined by MALDI-TOF mass spectrometry and showed a high catalytic activity (data not showed). Although homologous with mammalian or snake venom group I PLA(2)s, Anch TX-I and II is sufficiently ...201222100907
Patterns of range-wide genetic variation in six North American bumble bee (Apidae: Bombus) species.The increasing evidence for population declines in bumble bee (Bombus) species worldwide has accelerated research efforts to explain losses in these important pollinators. In North America, a number of once widespread Bombus species have suffered serious reductions in range and abundance, although other species remain healthy. To examine whether declining and stable species exhibit different levels of genetic diversity or population fragmentation, we used microsatellite markers to genotype popul ...201122035452
interspecific geographic distribution and variation of the pathogens nosema bombi and crithidia species in united states bumble bee populations.several bumble bee (bombus) species in north america have undergone range reductions and rapid declines in relative abundance. pathogens have been suggested as causal factors, however, baseline data on pathogen distributions in a large number of bumble bee species have not been available to test this hypothesis. in a nationwide survey of the us, nearly 10,000 specimens of 36 bumble bee species collected at 284 sites were evaluated for the presence and prevalence of two known bombus pathogens, th ...201122119631
revisiting comparisons of genetic diversity in stable and declining species: assessing genome-wide polymorphism in north american bumble bees using rad sequencing.genetic variation is of key importance for a species' evolutionary potential, and its estimation is a major component of conservation studies. new dna sequencing technologies have enabled the analysis of large portions of the genome in nonmodel species, promising highly accurate estimates of such population genetic parameters. restriction site-associated dna sequencing (radseq) is used to analyse thousands of variants in the bumble bee species bombus impatiens, which is common, and bombus pensyl ...201424351120
diagnosis of hymenoptera venom sensitivity.the objective of this review is to highlight recent advances in the preparation, documentation and performance of reagents and methods used in the diagnosis of hymenoptera-venom-induced immediate-type hypersensitivity.200212130950
occupational allergy to bumblebees: allergens of bombus terrestris.with the increase in commercial vegetable production in greenhouses, occupational sensitization to bumblebee venom is becoming more common. studies using sera from subjects thus sensitized allow evaluation of the allergenic specificity of bumblebee sensitization.200111692115
the effect of petal size manipulation on pollinator/seed-predator mediated female reproductive success of hibiscus moscheutos.the effects of petal-size manipulations on the behavior of pollinators and pollen/seed predators, and on pollen removal and deposition, were studied in hibiscus moscheutos (malvaceae) populations. the ultimate effects on the female reproductive success of flowers, such as fruit set, seed predation rate, and final seed set were also measured. we applied three levels of petal removal (100%, 50%, and 0% size reduction in radius) to flowers in natural populations. two pollinators (bombus pennsylvani ...199828308508
interaction of bombolitin iii with phospholipid monolayers and liposomes and effect on the activity of phospholipase a2.this study is focused on the characterization of the interaction of the amphiphilic peptide bombolitin iii (from the bumblebee megabombus pennsylvanicus) with phospholipid monolayers and vesicles. it is shown that due to the amphiphilic character of its alpha-helical conformation this water-soluble peptide is able to interact in an ordered fashion with phospholipid organized structures. depending on the temperature, the subphase, and the particular phosphatidylcholine used, the mixed peptide-pho ...19948204603
allergy to bumblebee venom. ii. ige cross-reactivity between bumblebee and honeybee venom.to obtain more information on ige cross-reactivity between bumblebee venom and honeybee venom, we tested sera from venom-sensitized patients for specific ige against venoms from the european bumblebee (bombus terrestris), the north american bumblebee (megabombus pennsylvanicus), and the honeybee (apis mellifera). rast, rast-inhibition, and immunoblotting experiments indicate that bumblebee venom and honeybee venom contain venom-specific ige-binding epitopes. these results suggest that immunother ...19989722226
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