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responses of maturing larvae of anastrepha suspensa to light and to immersion in water. 19744443467
biosteres longicaudatus: developmental dependence on host (anastrepha suspensa) physiology. 19827200914
a functional analysis of the p-element gene-transfer vector in insects.a p-element mobility excision assay was used to determine if non-drosophilid insects could support p gene vector function. present studies included the testing of muscids, sphaerocerids, and phorids, none of which were able to support p mobility. a new excision indicator plasmid was developed allowing the detection and recovery of virtually all p-element excision products. the frequency and sequence analysis of excision products from drosophila melanogaster and another drosophilid, chymomyza pro ...19938385510
developmental regulation of yolk protein gene expression in anastrepha suspensa.a partial cdna clone for the 48,000 dalton yolk polypeptide gene from anastrepha suspensa was isolated from a cdna expression library using a yolk polypeptide antibody probe and hybridization to the drosophila melanogaster yolk protein 1 gene. the sequenced dna has greatest homology to the yolk protein genes from ceratitis capitata, d. melanogaster, and calliphora erythrocephala and, similar to these genes, shares amino acid sequence domains with those from lipases. rna hybridization studies ind ...19979243791
sex pheromone components in oral secretions and crop of male caribbean fruit flies, anastrepha suspensa (loew).chemical analysis of hexane extracts of the oral secretions from male caribbean fruit flies, anastrepha suspensa, resulted in identification of pheromone components including: anastrephin, epianastrephin, suspensolide, beta-bisabolene, and alpha-farnesene in a ratio of 63:396:4:8:1. extracts of the crop from male flies contained these same components. no pheromone was detected in the extracts of female oral secretions. bioassay of the oral secretions indicated that females were attracted to oral ...200111673844
quantifying individual fruit fly consumption with anastrepha suspensa (diptera: tephritidae).we needed a technique to compare the consumption of baits by individual carribbean fruit fly, anastrepha suspensa (loew). by improving consumption and determining individual dose, we could lower pesticide concentration while retaining bait/pesticide efficacy and potentially reduce the environmental impact of fruit fly bait/pesticide eradication methods. we report here a precise dye-based technique for the quantification of consumption by individual adult a. suspensa fruit flies. fluorescein, mea ...200415666736
plasticity in mrna expression and localization of orthodenticle within higher diptera.orthodenticle (otd) genes are found throughout the animal kingdom and encode well-studied homeodomain transcription factors that share conserved functions in cephalization, head segmentation, brain patterning, and the differentiation of photoreceptors. otd proteins have been proposed as ancestral key players in anterior determination despite a high level of variation in gene expression at early developmental stages: otd is expressed strictly zygotically in the dipteran drosophila melanogaster, w ...200819021740
toxicity of imidacloprid-treated spheres to caribbean fruit fly, anastrepha suspensa (diptera: tephritidae) and its parasitoid diachasmimorpha longicaudata (hymenoptera: braconidae) in the laboratory.no-choice cage tests were used to study the toxicity of imidacloprid-treated spheres to caribbean fruit fly, anastrepha suspensa (loew), and its associated parasitoid, diachasmimorpha longicaudata (ashmead), in the laboratory. three imidacloprid sphere treatments (2, 4, and 8% active ingredient [ai] provado 1.6 f) and an untreated control sphere (no toxicant) were evaluated against a. suspensa. throughout the observation period (2-72 h), all concentrations of imidacloprid-treated spheres killed ...200415154477
laboratory and field cage studies on female-targeted attract-and-kill bait stations for anastrepha suspensa (diptera: tephritidae).development of attract-and-kill bait stations for pest fruit flies has been limited by the water solubility of sugar needed as a feeding stimulant and by the volatility of chemical attractants. a wax-based matrix was developed that provides the longevity needed for field use and is biodegradable.200919306314
grapefruit gland oil composition is affected by wax application, storage temperature, and storage time.the effect of wax application, storage temperature (4 or 21 degrees c), and storage time (14 or 28 days after wax application) on grapefruit gland oil composition was examined by capillary gas chromatography. wax application decreases nonanal and nootkatone levels. beta-pinene, alpha-phellandrene, 3-carene, ocimene, octanol, trans-linalool oxide, and cis-p-mentha-2,8-dien-1-ol levels increase, but limonene levels decrease, with temperature. levels of alpha-pinene, limonene, linalool, citronellal ...199910552497
characterization of the proteasomebeta2 subunit gene and its mutant allele in the tephritid fruit fly pest, anastrepha suspensa.in drosophila melanogaster the beta2 proteasome subunit gene, prosbeta2, was first identified as a dominant temperature sensitive mutant, dts-7, that causes pupal lethality at 29 degrees c but allows survival to adulthood at 25 degrees c. to explore the use of proteasome mutations for a conditional lethal system in insect pests, we identified and isolated the beta2 subunit gene of the 20s proteasome from the caribbean fruit fly, anastrepha suspensa. the caribfly ortholog asprosbeta2 was isolated ...200919523065
influence of methoprene and dietary protein on male anastrepha suspensa (diptera:tephritidae) mating aggregations.the caribbean fruit fly, anastrepha suspensa (loew), like many polyphagous tephritids, exhibits a lek polygyny mating system, and juvenile hormone levels and adult diet are known to have important positive effects on male sexual success. among the potential components of this success are male lek tenure and female response to the sexual signals of lekking males. male a. suspensa where submitted to four different treatments: (m(+)p(+)) application of juvenile hormone analog, methoprene (m) and su ...200919185584
larval feeding substrate and species significantly influence the effect of a juvenile hormone analog on sexual development/performance in four tropical tephritid flies.the juvenile hormone (jh) analog methoprene reduces the amount of time it takes laboratory-reared anastrepha suspensa (caribbean fruit fly) males to reach sexual maturity by almost half. here, we examined if methoprene exerted a similar effect on four other tropical anastrepha species (anastrepha ludens, anastrepha obliqua, anastrepha serpentina and anastrepha striata) reared on natural hosts and exhibiting contrasting life histories. in the case of a. ludens, we worked with two populations that ...200919101560
response of anastrepha suspensa (diptera: tephritidae) to terminal diamines in a food-based synthetic attractant.a current trapping system for anastrepha fruit flies uses a two-component food-based synthetic attractant consisting of ammonium acetate and putrescine (1,4-diaminobutane). development of more effective monitoring programs may be realized through identification of additional attractant chemicals. this study examined response of the caribbean fruit fly, anastrepha suspensa (loew), to putrescine and four homologous terminal diamines, differing only in carbon chain length. using a fixed dose of eac ...200819036190
effective sampling range of food-based attractants for female anastrepha suspensa (diptera: tephritidae).release-recapture studies were conducted with both feral and sterile females of the caribbean fruit fly, anastrepha suspensa (loew) (diptera: tephritidae), to determine sampling range for a liquid protein bait (torula yeast/borax) and for a two-component synthetic lure (ammonium acetate and putrescine). tests were done in a guava, psidium guajava l., grove and involved releasing flies at a central point and recording the numbers captured after 7 h and 1, 2, 3, and 6 d in an array of 25 multilure ...201020429471
enhancing male sexual success in a lekking fly (anastrepha suspensa diptera: tephritidae) through a juvenile hormone analog has no effect on adult mortality.while defending lek-territories, male anastrepha suspensa (loew) produce chemical, acoustic and visual courtship signals. in the laboratory and under semi-natural conditions, topical application of the juvenile hormone analog methoprene doubles pheromone production and subsequently doubles sexual success. however, sexual signals and interactions are likely to be physiologically expensive and so result in higher male mortality. comparison of males kept in isolation for 35 days, but provided daily ...201020470780
consumption of sugars by anastrepha suspensa (diptera: tephritidae).we were interested in determining the feeding response of the caribbean fruit fly, anastrepha suspensa loew (diptera: tephritidae), to various sugar concentrations to develop an improved bait for adults. we compared the consumption of 0.01-1.00 m concentrations of glucose, fructose, raffinose, and sucrose in no-choice tests for 24-h- and 6-d-old male and female flies. sucrose was the most consumed sugar or within the most consumed group of sugars at 0.02-0.20 m concentrations. there were no diff ...200718232414
a light and electron microscopical study of the spermathecae and ventral receptacle of anastrepha suspensa (diptera: tephritidae) and implications in female influence of sperm storage.female insects with multiple sperm storage organs may potentially influence patterns of paternity by differential storage of sperm from competing males. the caribbean fruit fly, anastrepha suspensa, stores sperm differentially with respect to its three spermathecae. to understand the mechanisms and processes responsible for patterns of sperm storage and use in a. suspensa, details of the fine structure of female sperm storage organs were resolved by uv-light microscopy, confocal microscopy, tiss ...200218088963
analysis of host preference and geographical distribution of anastrepha suspensa (diptera: tephritidae) using phylogenetic analyses of mitochondrial cytochrome oxidase i dna sequence data.anastrepha suspensa (loew) is an economically important pest, restricted to the greater antilles and southern florida. it infests a wide variety of hosts and is of quarantine importance in citrus, a multi-million dollar industry in florida. the observed recent increase in citrus infested with a. suspensa in florida has raised questions regarding host-specificity of certain populations and genetic diversity of the pest throughout its geographical distribution. cytochrome oxidase i (coi) dna seque ...200617092357
field comparison of chemical attractants and traps for caribbean fruit fly (diptera: tephritidae) in florida citrus.field studies in citrus were conducted to compare the following as attractants for the caribbean fruit fly, anastrepha suspensa (loew): torula yeast-borax; propylene glycol (10%); a two-component lure consisting of ammonium acetate and putrescine; a two-component lure consisting of ammonium bicarbonate and putrescine; and a three-component lure consisting of ammonium bicarbonate, methylamine hydrochloride, and putrescine. various combinations of these attractants in glass mcphail, plastic mcphai ...200516334334
purification and partial characterization of an entomopoxvirus (dlepv) from a parasitic wasp of tephritid fruit flies.an insect poxvirus [entomopoxvirus (epv)] occurs in the poison gland apparatus of female diachasmimorpha longicaudata, a parasitic wasp of the caribbean fruit fly, anastrepha suspensa and other tephritid fruit flies. the dlepv virion is 250-300 nm in diameter, has a "bumpy" appearance and a unipartite double stranded dna genome of 290-300 kb. dlepv dna restriction fragment profiles differed from those reported for amsacta moorei epv (amepv) and melanoplus sanguinipes epv (msepv), the only two ep ...200215455044
the beta2-tubulin gene from three tephritid fruit fly species and use of its promoter for sperm marking.to isolate testis-specific regulatory dna that could be used in genetically transformed insect pest species to improve their biological control, beta2-tubulin genes and their proximal genomic dna were isolated from three economically important tephritid pest species, anastrepha suspensa, anastrepha ludens, and bactrocera dorsalis. gene isolation was first attempted by degenerate pcr on an a. suspensa adult male testes cdna library, which fortuitously isolated the 2.85 kb beta1-tubulin gene that ...200919520163
forest fragments as barriers to fruit fly dispersal: anastrepha (diptera: tephritidae) populations in orchards and adjacent forest fragments in puerto rico.mcphail-type traps baited with ammonium acetate and putrescine were used to monitor populations of anastrepha obliqua (macquart) and anastrepha suspensa (loew) in two orchards with hosts of these flies (mango, mangifera indica l., and carambola, averrhoa carambola l.), as well as in forest fragments bordering these orchards. contour maps were constructed to measure population distributions in and around orchards. our results indicate that anastrepha populations are focused around host fruit in b ...201323575019
mitochondrial dna restriction map for the caribbean fruit fly, anastrepha suspensa, and occurrence of mitochondrial dna diversity within highly inbred colonies.a restriction map has been constructed for anastrepha suspensa mitochondrial dna. one haeiii site was found to be polymorphic among individuals in highly inbred colonies and a feral population. based on mapping information, the polymorphic site was determined to be in the atpase 6 gene. primers tk-j-3804 and c3-n-5460 amplified this region. the amplicon was cut by haeiii in flies of one haplotype and not cut in flies of the other haplotype. from 30 to 43% of the individual flies studied had this ...200212296630
transformation of the caribbean fruit fly, anastrepha suspensa, with a piggybac vector marked with polyubiquitin-regulated gfp.germ-line transformation was achieved in the caribbean fruit fly, anastrepha suspensa, using a piggybac vector marked with an enhanced green fluorescent protein gene regulated by the drosophila melanogaster polyubiquitin promoter. four transgenic g(0) lines were selected exhibiting unambiguous gfp expression. southern hybridization indicated the presence of one to four integrations in each of the transgenic lines with two integrations verified as piggybac-mediated by sequencing their insertion s ...200111164342
sodium tetraborate effects on mortality and reproduction of anastrepha suspensa (diptera: tephritidae).when flies were treated with 0- 0.5% sodium tetraborate by feeding for 24 h, mortality in treatments was not different from controls. fecundity and fertility were reduced by 0.5% sodium tetraborate. when flies were fed for 48 h, mortality of both males and females increased in the 0.5% sodium tetraborate treatment; oviposition was eliminated for 20 d after treatment. when treatment was extended to 168 h, 0.1% sodium tetraborate caused increased mortality and decreased fecundity and fertility. fe ...200011057722
mating experience and juvenile hormone enhance sexual signaling and mating in male caribbean fruit flies.young mated male caribbean fruit flies [anastrepha suspensa (loew)] have greater sexual prowess than their virgin counterparts. after mating for the first time, 6- to 7-day-old males released twice as much sex pheromone and acquired another mate in less than half the time required by virgin males of the same age. mass spectroscopic analysis of extracts of hemolymph from mated and virgin 7-day-old males resulted in identification of juvenile hormone iii bisepoxide and juvenile hormone iii in a ra ...200010706642
analysis and quantitation of insect juvenile hormones using chemical ionization ion-trap mass spectrometry.a method for identification and quantitation of insect juvenile hormones (jh) has been developed using capillary gas chromatography-chemical ionization (isobutane)-ion-trap mass spectroscopy. the method does not require derivatization of samples or use of selected ion monitoring. analysis over a mass range of 60-350 u allowed for identification of as little as 0.01 pmol of individual jh homologs. quantitative analysis was based on the ion intensities of six diagnostic ions and the summed intensi ...200010625507
isolation and identification of terpenoid sex pheromone components from extracts of hemolymph of males of the caribbean fruit fly.extracts obtained from hemolymph of sexually mature males of the caribbean fruit fly anastrepha suspensa contained four biologically important terpenoid components of the sex pheromone. the four components were identified as farnesene, bisabolene, anastrephin, and epianastrepin based on their relative retention indexes from capillary gas chromatography analysis, using both apolar and polar phase columns and their chemical ionization (isobutane) mass spectra. the ratio of the components in extrac ...199910578112
electroantennogram and behavioral responses of anastrepha suspensa (diptera: tephritidae) to putrescine and ammonium bicarbonate lures.at present, the most effective synthetic lures for pest anastrepha fruit flies are multicomponent blends that include ammonia and the diamine synergist putrescine (1,4-diaminobutane). both chemicals generally have been regarded as protein cues that result in female-biased attraction. using electroantennography (eag) and flight tunnel bioassays, this study evaluated response of the caribbean fruit fly, anastrepha suspensa (loew) to vapors released from commercial lure formulations of ammonium bic ...200919689908
a 24 kda parasitism-specific protein from the caribbean fruit fly, anastrepha suspensa: cdna and deduced amino acid sequence.a 24 kda parasitism-specific protein (psp24) was previously reported from the hemolymph of the caribbean fruit fly, anastrepha suspensa (diptera: tephritidae) after parasitization by the wasp diachasmimorpha longicaudata (hymenoptera: braconidae). this study was designed to sequence the open reading frame of psp24 and to determine whether it is encoded by the wasp, fruit fly host or by the entomopoxvirus d1epv which is normally injected into the host with the wasp's egg. utilizing an existing pa ...199910510497
an embryonic cell line from the caribbean fruit fly, anastrepha suspensa (diptera: tephritidae) 199910475248
the hobo transposable element excises and has related elements in tephritid species.function of the drosophila melanogaster hobo transposon in tephritid species was tested in transient embryonic excision assays. wild-type and mutant strains of anastrepha suspensa, bactrocera dorsalis, b. cucurbitae, ceratitis capitata, and toxotrypana curvicauda all supported hobo excision or deletion both in the presence and absence of co-injected hobo transposase, indicating a permissive state for hobo mobility and the existence of endogenous systems capable of mobilizing hobo. in several str ...19968807305
purification of a 24 kd parasitism-specific hemolymph protein from pharate pupae of the caribbean fruit fly, anastrepha suspensa.we report purification of a 24 kd parasitism-specific protein (24 kd psp) from pharate pupal hemolymph of the caribbean fruit fly, anastrepha suspensa, after parasitization by the braconid wasp, diachasmimorpha (= biosteres) longicaudata. we previously utilized isoelectric focusing (ief) and two-dimensional (2-d) gel electrophoresis to demonstrate that the 24 kd psp consists of two variants with pl 6.7 (more abundant) and pl 6.3. purification of the more abundant 24 kd psp variant was accomplish ...19947803811
patterns of inner chorion structure in anastrepha (diptera: tephritidae) eggs.the inner chorion structure of anastrepha eggs from 16 species of various infrageneric taxonomic groups is described by scanning and transmission electron microscopy. the layers of the chorion, the outer egg membrane, are structurally similar. furthermore, an additional trabecular layer (atl) that exists in some species, together with other characteristics, facilitates the recognition of four patterns of chorion structuring: pattern i, in which the atl layer is absent, is found in anastrepha ami ...201727847335
identifying anastrepha (diptera; tephritidae) species using dna barcodes.molecular identification of fruit flies in the genus anastrepha (diptera; tephritidae) is important to support plant pest exclusion, suppression, and outbreak eradication. morphological methods of identification of this economically important genus are often not sufficient to identify species when detected as immature life stages. dna barcoding a segment of the mitochondrial cytochrome oxidase i gene has been proposed as a method to identify pests in the genus. the identification process for the ...201729202187
use of the electroretinogram to measure the quality of vision of the fruit fly.the electroretinogram (erg) technique was used to measure the visual sensitivity of caribbean fruit flies anastrepha suspensa (loew), reared at 3 laboratories on 5 artificial diets. these ergs were compared with those of flies reared from field-infested fruit. flies reared on artificial diets differed greatly in visual sensitivity, and those reared on fruit were at least 10 times more sensitive than certain strains. since ergs can be obtained 3-4 days before the caribbean fruit fly is sexually ...19751204589
glucosylated suspensosides, water-soluble pheromone conjugates from the oral secretions of male anastrepha suspensa.a diastereomeric mixture of the glycosylated pheromones (6r)-(1a) and (6s)-beta-d-glucopyranosyl 2-(2,6-dimethyl-6-vinylcyclohex-1-enyl)acetate (1b), which we named respectively suspensoside a and suspensoside b, was isolated from the oral secretions of male caribbean fruit flies, anastrepha suspensa. the absolute stereochemical configurations were established using microsample nmr instrumentation, chiral gas chromatography, and chemical synthesis utilizing pure enantiomers of anastrephin, (3as, ...200818798680
Male only progeny in Anastrepha suspensa by RNAi-induced sex reversion of chromosomal females.In Tephritidae sex determination is established by orthologs to the Drosophila melanogaster transformer and transformer-2 genes, though the primary signals for sex determination differ. The presence of the Y chromosome in the tephritid species is critical for male differentiation, while the ratio of X chromosomes to autosome ploidy is critical in drosophilids. Here the isolation, expression and function of tra and tra-2 orthologs are described for the agriculturally important tephritid, Anastrep ...201222079281
a compound produced by fruigivorous tephritidae (diptera) larvae promotes oviposition behavior by the biological control agent diachasmimorpha longicaudata (hymenoptera: braconidae).tephritid fruit fly parasitoids use fruit-derived chemical cues and the vibrations that result from larval movements to locate hosts sequestered inside fruit. however, compounds produced by the larvae themselves have not been previously described nor their significance to parasitoid foraging determined. we collected the volatiles from four species of tropical and subtropical tephritidae: anastrepha suspensa (loew), bactrocera dorsalis hendel, bactrocera cucurbitae coquillett, and ceratitis capit ...201122251652
long aculeus and behavior of anastrepha ludens render gibberellic acid ineffective as an agent to reduce 'ruby red' grapefruit susceptibility to the attack of this pestiferous fruit fly in commercial groves.treating mexican grapefruit with gibberellic acid (ga3) before color break, significantly delayed peel color change and increased peel puncture resistance, but it did not reduce grapefruit susceptibility to mexican fruit fly, anastrepha ludens (loew) attack under natural conditions. despite ga3 treatments, larval infestation levels increased with higher fruit fly populations, which also increased as the season progressed. late in the season, infestation levels were even higher in ga3-treated fru ...200616937671
transmission of the diachasmimorpha longicaudata rhabdovirus (dlrhv) to wasp offspring: an ultrastructural analysis.during oviposition, the parasitic wasp diachasmimorpha longicaudata introduces an entomopoxvirus (dlepv) and a rhabdovirus (dlrhv) into larvae of its tephritid fruit fly host anastrepha suspensa. dlepv and dlrhv replicate, respectively, in host hemocytes and epidermal cells. both viruses, like many beneficial viruses of parasitic wasps, are retained in all wasp generations but their avenue(s) of transmission are unknown. this study tests the hypothesis that dlrhv is transmitted transovarially or ...200515749107
morphogenesis and cytopathic effects of the diachasmimorpha longicaudata entomopoxvirus in host haemocytes.the diachasmimorpha longicaudata entomopoxvirus (dlepv), the first reported symbiotic entomopoxvirus, occurs in the venom apparatus of d. longicaudata female wasps and is introduced into anastrepha suspensa larvae during parasitism. the dlepv 250-300 kb double stranded dna genome encodes putative proteins having 30 to >60% amino acid identity with poxvirus homologs such as dna helicase, dna-dependent rna polymerase, and the poxvirus-specific rifampicin resistance protein. although the molecular ...200515749106
influence of a juvenile hormone analog and dietary protein on male anastrepha suspensa (diptera: tephritidae) sexual success.juvenile hormone levels and adult diet have important effects on the attractiveness and competitiveness of male anastrepha suspensa (loew) (caribbean fruit fly). because the success of the sterile insect technique requires the release of males that can compete in the wild, these effects are of crucial importance. laboratory and field cage experiments were conducted to compare male sexual performance on a lifetime basis and daily basis when submitted to four different treatments: (m+p+) applicati ...201020214366
a homolog of the vaccinia virus d13l rifampicin resistance gene is in the entomopoxvirus of the parasitic wasp, diachasmimorpha longicaudata.the parasitic wasp, diachasmimorpha longicaudata (ashmead) (hymenoptera: braconidae), introduces an entomopoxvirus (dlepv) into its caribbean fruit fly host, anastrepha suspensa. (loew) (diptera: tephritidae), during oviposition. dlepv has a 250-300 kb unipartite dsdna genome, that replicates in the cytoplasm of the host's hemocytes, and inhibits the host's encapsulation response. the putative proteins encoded by several dlepv genes are highly homologous with those of poxviruses, while others ap ...200820345294
grape juice as a bait for anastrepha suspensa (diptera: tephritidae) and zaprionus indianus (diptera: drosophilidae).in field tests conducted in south florida to test grape juice as a bait for the caribbean fruit fly, anastrepha suspensa loew, high numbers of zaprionus indianus gupta were captured in traps with aqueous grape juice. these experiments included comparisons of grape juice bait with established a. suspensa protein-based baits (ammonium acetate + putrescine lures, or torula yeast) or wine, a bait found previously to be attractive to z. indianus. effects of different preservatives (polypropylene glyc ...201526470354
pro-apoptotic gene regulation and its activation by gamma-irradiation in the caribbean fruit fly, anastrepha suspensa.transcriptional activation of pro-apoptotic genes in response to cytotoxic stimuli is a conserved feature of the cell death pathway in metazoans. however, understanding the extent of this conservation in insects has been limited by the lack of known pro-apoptotic genes in non-drosophilids. recently, we described the pro-apoptotic genes, asrpr and ashid, from the tephritid, anastrepha suspensa, that now allow us to explore the conservation of pro-apoptotic gene regulation between a tephritid and ...201525433919
an est database of the caribbean fruit fly, anastrepha suspensa (diptera: tephritidae).invasive tephritid fruit flies are a great threat to agriculture worldwide and warrant serious pest control measures. molecular strategies that promote embryonic lethality in these agricultural pests are limited by the small amount of nucleotide sequence data available for tephritids. to increase the dataset for sequence mining, we generated an est database by 454 sequencing of the caribfly, anastrepha suspensa, a model tephritid pest. this database yielded 95,803 assembled sequences with 24% id ...201323296060
strategy for enhanced transgenic strain development for embryonic conditional lethality in anastrepha suspensa.here the first reproductive sterility system for the tephritid fruit fly pest, anastrepha suspensa, is presented, based on lethality primarily limited to embryos heterozygous for a conditional lethal transgene combination. this tetracycline (tet)-suppressible system uses a driver construct having the promoter from the newly isolated embryo-specific a. suspensa serendipity α gene linked to the tet-transactivator. this was used to drive expression of a phosphomutated variant of the pro-apoptotic c ...201222647610
short-term anoxic conditioning hormesis boosts antioxidant defenses, lowers oxidative damage following irradiation and enhances male sexual performance in the caribbean fruit fly, anastrepha suspensa.most organisms are repeatedly exposed to oxidative stress from multiple sources throughout their lifetimes, potentially affecting all aspects of organismal performance. here we test whether exposure to a conditioning bout of anoxia early in adulthood induces a hormetic response that confers resistance to oxidative stress and enhances male sexual performance later in life in the caribbean fruit fly, anastrepha suspensa. anoxic conditioning of adults prior to emergence led to an increase in antiox ...201222623204
genetic variation of anastrepha suspensa (diptera: tephritidae) in florida and the caribbean using microsatellite dna markers.anastrepha suspensa (loew) (diptera: tephritidae), the caribbean fruit fly, is indigenous to florida and the greater antilles where it causes economic losses in fruit crops, including citrus. because of the geographic separation of many of its native locations and anecdotal descriptions of regional differences in host preferences, there have been questions about the population structure of a. suspensa. seven dna microsatellite markers were used to characterize the population genetic structure of ...201021309246
a functional comparison of the 3xp3 promoter by recombinase-mediated cassette exchange in drosophila and a tephritid fly, anastrepha suspensa.transposable elements are widely used as vectors for integrating transgenes into the genome of insects. however, the random nature of transposon vector integrations often results in mutations and makes transgene expression subject to variable genomic position effects. this makes reliable quantitative comparisons of different transgenes difficult and development of highly fit transgenic strains laborious. tools for site-specific transgene targeting are essential for functional genomic comparisons ...201323550127
pro-apoptotic cell death genes, hid and reaper, from the tephritid pest species, anastrepha suspensa.pro-apoptotic proteins from the reaper, hid, grim (rhg) family are primary regulators of programmed cell death in drosophila due to their antagonistic effect on inhibitor of apoptosis (iap) proteins, thereby releasing iap-inhibition of caspases that effect apoptosis. using a degenerate pcr approach to conserved domains from the 12 drosophila species, we have identified the first reaper and hid orthologs from a tephritid, the caribfly anastrepha suspensa. as-hid is the first identified non-drosop ...201121630017
a bioassay system for collecting volatiles while simultaneously attracting tephritid fruit flies.a bioassay system was developed that permits the testing of various substrates for biological activity in a flight tunnel, while simultaneously collecting a portion of the volatiles from the attractive source for subsequent chemical identification and quantification. bioassays of the response of virgin female caribbean fruit flies,anastrepha suspensa (loew) (diptera: tephritidae), to volatiles released by calling males were conducted in a greenhouse under natural light cycles and fluctuating env ...199324248585
host vibration - a cue to host location by the parasite, biosteres longicaudatus.biosteres longicaudatus ashmead (hymenoptera: bracon dae) is a solitary endoparasite of anastrepha suspensa larvae (diptera: tephritidae), which live in fruit tissue. larvae make andible noises within macerated fruit or larval medium in which they are reared. parasite females readily located normal, mobile larvae and spent a mean of 16.5±4.7 min/visit to parasitize these hosts. in contrast, females were unable to locate etherized or dead hosts and abandoned them after only 1.9±0.9 and 2.3±0.8 mi ...198128309807
a transgenic embryonic sexing system for anastrepha suspensa (diptera: tephritidae).the sterile insect technique (sit) is a highly successful biologically-based strategy to control pest insect populations that relies on the large-scale release of sterilized males to render females in the field non-reproductive. for medfly, a mutant-based sexing system is available as well as a transgenic system where a tetracycline-suppressible (tet-off) toxic molecule is female-specifically produced. however, the former classical genetic system took many years to refine, and the latter system ...201222858603
hormetic benefits of prior anoxia exposure in buffering anoxia stress in a soil-pupating insect.oxygen is essential for most animals and exposure to a complete lack of oxygen, i.e. anoxia, can result in irreparable damage to cells that can extend up to the organismal level to negatively affect performance. while it is known that brief anoxia exposure may confer cross-tolerance to other stressors, few data exist on the biochemical and organismal consequences of repeated intermittent bouts of anoxia exposure. in nature, the caribbean fruit fly anastrepha suspensa (loew)(diptera: tephritidae) ...201829367272
early life hormetic treatments decrease irradiation-induced oxidative damage, increase longevity, and enhance sexual performance during old age in the caribbean fruit fly.early life events can have dramatic consequences on performance later in life. exposure to stressors at a young age affects development, the rate of aging, risk of disease, and overall lifespan. in spite of this, mild stress exposure early in life can have beneficial effects on performance later in life. these positive effects of mild stress are referred to as physiological conditioning hormesis. in our current study we used anoxia conditioning hormesis as a pretreatment to reduce oxidative stre ...201424498251
tetracycline-suppressible female lethality and sterility in the mexican fruit fly, anastrepha ludens.the sterile insect technique (sit) involves the mass release of sterile males to suppress insect pest populations. sit has been improved for larval pests by the development of strains for female-specific tetracycline-suppressible (tet-off) embryonic lethal systems for male-only populations. here we describe the extension of this approach to the mexican fruit fly, anastrepha ludens, using a tet-off driver construct with the tet-transactivator (tta) under embryo-specific anastrepha suspensa serend ...201627135433
y-linked markers for improved population control of the tephritid fruit fly pest, anastrepha suspensa.insect pest control programs incorporating the sterile insect technique (sit) rely on the mass production and release of sterilized insects to reduce the wild-type population through infertile matings. most effective programs release only males to avoid any crop damage caused by female fruit flies or transmission of disease by female mosquitoes. therefore, the females have to be eliminated, preferably in an early developmental stage, during mass rearing. different systems and techniques have bee ...201323719710
quantitative analysis of pheromone production in irradiated caribbean fruit fly males,anastrepha suspensa (loew).pheromonal volatiles emitted by irradiated and control 5- to 11-day-old caribbean fruit flies,anastrepha suspensa (loew), were collected on tenax adsorbent filters and quantified by gas chromatography (gc). the components released were identified by comparison of retention times on gc and by mass spectrometry (ms) with authentic synthetic standards. pharate adults were irradiated with gamma rays from a(60)co source at a dose rate of 10.3 gray (gy)/min. the total dosages given were 30, 50, 70, an ...199324248794
biological control potential of entomopathogenic nematodes for management of caribbean fruit fly, anastrepha suspensa loew (tephritidae).caribbean fruit fly (caribfly) is a serious economic insect pest because of development of larvae that hatch from eggs oviposited into fruits by female adults. this study assessed the virulence of twelve entomopathogenic nematode (epn) isolates to caribfly in laboratory bioassays as a starting point toward evaluation of management strategies for the fruit-to-soil-dwelling stages of a. suspensa in fields infested by caribfly.201627717178
caribbean fruit fly,anastrepha suspensa (loew), attraction to host fruit and host kairomones.extracts of 22 fruits were tested for their attractancy toanastrepha suspensa (loew), the caribbean fruit fly. box-orange, calamondin, carambola, cattley guava, loquat, and surinam-cherry were about equal in attractiveness to males and females. nine synthetic chemicals, including four found in box-orange ripe seed, were attractive to females. five synthetic chemicals, including two in box-orange ripe seed, were attractive to males. farnesol,α-phellandrene, and 3-carene were highest in attractive ...199424242123
biology of pheromone release by male caribbean fruit flies,anastrepha suspensa (diptera: tephritidae).males of the caribbean fruit fly,anastrepha suspensa (loew), typically form leks and attract females by releasing a multicomponent volatile pheromone. previous reports have identified two nine-carbon alcohols, three lactones, a sesquiterpene, and a monoterpene in the volatiles. the present report is a study of the physiology of male pheromone release and of ecological and social interactions that influence pheromone release by laboratoryreared flies. volatiles released by males were trapped on t ...199024263511
intraspecific competition among first instars of the parasitic wasp biosteres longicaudatus.females of the solitary endoparasitic wasp biosteres longicaudatus sometimes deposit >1 egg (superparasitism) in each larva of the caribbean fruit fly host, anastrepha suspensa. as host density increases, there is an inverse relationship between the level of superparasitism and the number of progeny produced/female. larval parasitoid competition in superparasitized hosts causes an abrupt decline from >1 to 1 or <1 parasitoid/host 24-36 h before the surviving parasitoid larva molts to the 2nd ins ...198828311769
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